RLG00000004623

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000526
Physical Location & Seq
Reverse (-)
60894682 .. 60901022
6341 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004623

Sequence Viewer

Length: 4560 bp
ATGGCGACGACGATGACGCCAAGGGAGCACATAGAAGATATACGGAAGACCAAGTTCTCCATCGGAGAAAAGCTCAACCCTTTAACGGAGGATCTTCACCAGGCCGTCAAGAACCTCTCCGGCGAGCTTTACGCTAAAGACGTCCACTTTTTCATGGAACTCGTTCAGAATGCAGAAGACAATGAATACTTGGAGGGGGTTGATCCATCCCTTGAGTTTGTGATTACATACCTTGATATAACGGCTACAGGGGCTCTAGCCACATTGCTGGTTTTCAATAATGAGAAGGGTGTGTTTCTGATCACTGCTCAGCCTTACATATTTAGTAATGGTTATCAGATAAGGTTCAGTGAAGAGCCTTGTTCACATTGCAATGTTGGCTACATAGTACCTGAATGGGTTGAGCAGAATCCCACACTATCTGATATAAAAAAGATATATGGTTCTGCTACTGCCCTTCCTACCACAACAGTGATCTTACCTCTGAAGCCCGACAAGGTTGAACCTGTGAAGAAACAACTCTCAATCATTCATCCAGAAGTTCTCTTGTTCCTATCAAAGATTAAGCGGCTCTCTGTTAGGGAAGACAAAGAGGAACCAAGGCAGAACACTGTGAGTGCAATTGAGATAGCAAGCAAGACTGAATTTGTGTCAAGGAAGAACATCGATGCTCAATCCTACACTCTGCATCTTTCAGCTGAAGAAAATGATGGAAATGGAGAATGCAGTTACTACATGTGGAAACAAAAGTTTCCAGTCAGGCAGGAATTCAGAGTTGAGAGGAGAAAGGAGGTGGATGAAGCAAGGAACTCTTCGCCTGGTGTTTATGCATTTCTTCCCACAGAGATGGTCACCAACTTTCCATTCATAATACAGGCGGATTTTCTTTTAGCATCTTCAAGAGAGACAATCCTACTTGATAACAAGTGGAATCAAGGGATTCTTGAGTGTGTGCCCACCGCATTTGCCCATGCTTTTAACTCTCTTGTGAAAACTGTGGAAGGTGCGCCAACATCAAGTTTGCCTCCTATGTTCAGATTCTTGCCTGTTCAGGGTTCTTCCTATAAAGAATTAAATGCTGTAAGGGAGTCAATAAAAGCAAAGATGGTTGACGAAGACATTATACCAGTTGAGCCTGAAATGGAGCAGAGGTTCTTCCACAAGCCTCGTGAAGTGGGAAGATTAATGCCTGCCTTTTGGACTATTTTGAGGAAGGCGAGGGTTCAAGGTGTAAGCTTGATTAAGCTTTCATCACATGGAAGATTTGTTCTGTCTTATTCCTTTGACAGCAAGGAATATGATCCTATACTAAGTTTCCTTGGAGTTGAACCAGTGAATGATGCCTGGTATGTTAAATGCATACAGGGTACTTCTGATCTTGTTGATGGAGTGTCAGAAGATGTGTACTTAGAGCTTCTACTGTTTATCGCTTACAATTGGGGTTCTAAATTTGGTCACACCAACATGAAGAACATACCATTGATAAAATATGCAAACTGTAGAAATGAGTCTTTATGCAGCATAAGCGAAATCCAGAAAGGTGAATGTAGAGTTTTCTTATCGAGCAAAAGTTCTCGTGTTTCATGGCTGATTGATTGCAACCGGGAATTTAATTCTGTGACTCGTCTTCTCTTTATGCCAAAAGCCACTCAGGAAGCCATCTGGGCATGCTCTGGGAAGGATTCATTGAAGAAATGGCTAGCTGATCAAGCTAAGGTTGGTTCTGTTGATGTCAATCAATATGCAGTTGATCTCCTTCAGAATTCTCTTAATGAGCGGAAGCTTGTTATTGCATATGTTCTTGGCCGTTTATGTGGAATTATGCCACTAGTGGATGGCTATGGGAACATCATCACAAGTAGAAAAGGCGTTGTTGTTCCTGCCAATGGAAGCAAATGGGCGGGATTGACTGATTCTAATTTGTGGAGAAAAGAAGGGCATGTTGAATTAGGGGAGCATTATATGTATTCTGGCTACTTTGCTGGTAAGTATACACCGGAGAAGAAGCTTCTTGAGTTCCTTATAAAACATGCTGCTGCTTTGGATGTCCCTCATATTTCTGCCCCAAGTGATGGTATCACGTCTCTATCTGCACCACTAACTAAGCAGAATGCCTTCTTGCTTTTGAATTGGATTCGTCAACAGAATTATAAAAGGGTTGGCATCCCTCAGAAGTTCTTGGCTTGCATAAAGGAAGGTAGTTGGCTGAAAGTTACTTTGAATGGCTCTCCTGGTTATAGGCCGCCATCCCAGTCATTTCTGCTTAACTCATCATGGGGAAATACATTGCAGAATGGATCCGTCTCTGTTGACATTCCATTGATTGATACGAGCTTTTATGGTGAGAGCATTAGTGGATACAAGGACGAGCTAAAGACAATTGGGGTCGTGTTTGAATATAGCGAAGCATGTGACTTCATTGGGAAGCATTTCATGTCTCTGGCAGCTTCATCCTCTTTAACTAGAGGAAATGTTGTCATCTTAATGCTCAACTGTATGGCTCTATCCAAGTCATCTGATAAACTTGTCAATGCATTGAAAGGAGCCAAGTGCCTGAAGACAAGTATTGGTTACAAGTTTCATCACGAATGGGGTTGCATTCTTCAGGTCGTCAATGGTCTTCCCGTGATTGATCATGAATTCTATGGGAGCAACATTTTCCGGTACATAAATGAACTGAAAAAAATTGGTGTGGTGGTTGATTTTGAAGGGGCTGCAAAAGTATTTGCTCGATGTTTCAGGCACTATGCATCATCATCTTCCATCACCAAGGAAAATGTTGCATCATTGCTTCTGTGTTACAGAGAGCTGAATGGAACTCCATATAAATTTCCTGCTGATCTTAGGAGCTGCATTGGTAAGGTCAAGTGGTTGCGCAGTCGTTTTGGTGATTACAGATGTCCAAGGGAGTGCATTCTCTTTAGTCCCGAGTGGGAATCTATATCCCCAATAACTCTGCTTCCTTTCATTGATGATTCTGACAGTTACTATGGAAAGGGCATACATGAGTACAGAAAAGAGTTGAAGAGCTTGGGGGTTGTCGTCGAATACAAGGAAGGAGTTAAGTTTGTGGCTTCTGGTCTTTGTCTCCCTAGGGATGCCAGCCGTATTTTTCCTGTAAATGCGCTTGCGTTGCTGGAATGCATACGTATTTTCGAGAAGAAAGGTTACTCCTTTCTTGATAATTCTGATTTCATGAAGAAAGTTTCTCAACCATGGATAAAGACTCATGCTGGTTATTATAGGTCTCCCAAAGAGTCTTTGTTGTTTGATTCCAAGTTTGGCTTATATTTGAAGCGGACTGACGGGCCTTTCATTGATGAAGAATTCTATGGTACTAAGATTGCATCTTACAAAAAAGAGCTGTCTGCATTGGGGGTTATTGTTGCAGCTGAGAAAGGAAGTCCCCTGATTGCCAGCCACCTTGATGTTCATTATGAGATTGAAGCATTTGTTCGGCTGTATAATTACTTGAGTGCATTCAAGTGGGAACCAGACACTGATGCTGACAGATGGATCTGGGTTCCAAAAGGAGAGTGGGTTCGTTCTGATGATTGTGTTATATATGACAAGGATGGGCTCTTTGGTTTACAATTGACTGTTTTGGAGAAACATTATGAACGGAAACCTCTTTTTTTCTTCTCCAGTGCGTTTGGAGTTAAATCTCATCCTTCAGTTGATGATTACTTGAAGGTTTGGAAGGTTTGGGAAGGTTCTCGAAGTGCATTGACGCATGCTGATTGTTGCAAGTTTTGGAGTTATATCTCGAAGCACTGGAATTCAAAGACAGAAAAGACTGTTGCTGATGCCTTGGTGAAAGTACCTGTCAGTTCTGGATCTGAAGGTATCTTGCTGTGCAACAAGTCTGATGTTTTCATTGCTGATGACCTTCAACTGAAGTATCTATTTGAACAGTCTTCTCCTGTGTCTATTTTTGTTTGGTACCCTCAGCCAAGTTTGCCTTCCATTCCAAGATCCAAGTTGCTTGACATTTATCGAAGAATTGGTGTTCGCACTATATCTGAATCTGTACAGAAGGAAGAGGCGTCACTTGCAGATGATGTTGAAGTAGTCTCTTTGCCAAGGGAAAAGCTGATTAAGAAAGCGCTGTTCAGGCTGATTCTTGGTTTTCTTGCTGCTCCTCCCATGGAAATGGAAGCTGAGAAGAGGCTTGAAGCTGTTCAATTCATTGTGAATGTTACTGTGGTGGTAACAGCTGAACCAATCTCTGTAAGCTGTAGTCTACCTTTATCTTCTTGGAAAGTTTTGAATGTGAGAGGAAGCCAAAAGATACGTTTTGATAGGGACAATTCAATGATCTTCACTCAGAAAATGGACAAGTCTGGAGGGCAGAAGAGTATGTTTGAGTTTGCTACTTCTTTCTCTCAAGCAATATCTGAGAGTGTGGTATGGGATAGTACGGATCATATAGATTCACTCTCTGAGCTGATCAAGGTGGCTGCTTTGCTAAACTTCAAAGAGGAAGCAGTTGATTCCTTGATGAAGTCCAAGAATTTGCAAATCTTCATGGAGGATGAGGAGTTTCTCAGGACTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1520

Amino Acids

171.84

Weight (kDa)

6.98

Isoelectric Point (pI)

39.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28020 AT3G48770 AT3G48770
fragaria_vesca FvH4_5g09020 FvH4_5g09020
malus_domestica MD06G1092400.v1.1 MD06G1134600.v1.1 MD06G1134700.v1.1 MD06G1134800.v1.1 MD06G1135200.v1.1 MD06G1135400.v1.1 MD06G1135500.v1.1 MD08G1137600.v1.1 MD15G1395100.v1.1
prunus_persica Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145600_v2.0.a1 Prupe.5G145800_v2.0.a1 Prupe.5G145900_v2.0.a1
pyrus_communis pycom06g12550 pycom06g12570 pycom06g12580 pycom06g12590 pycom06g12610 pycom06g12630 pycom06g12640 pycom06g12650 pycom08g06280
rosa_chinensis RchiOBHm_Chr6g0249321 RchiOBHm_Chr6g0275111 RchiOBHm_Chr7g0189191 RchiOBHm_Chr7g0189261
rosa_laevigata RLG00000004622 RLG00000004623 RLG00000008237 RLG00000008238 RLG00000023303 RLG00000035187
rosa_multiflora Rmu_co8294311.1_g000001 Rmu_co8389833.1_g000001 Rmu_sc0001044.1_g000004 Rmu_sc0001329.1_g000011 Rmu_sc0006012.1_g000010 Rmu_sc0007286.1_g000032 Rmu_sc0009360.1_g000016 Rmu_sc0011817.1_g000001 Rmu_sc0015050.1_g000001
rosa_roxburghii Rroxscaffold_1G00064000 Rroxscaffold_3G00265460 Rroxscaffold_3G00265580 Rroxscaffold_3G00265640 Rroxscaffold_3G00265690 Rroxscaffold_3G00265700 Rroxscaffold_7G00193620 Rroxscaffold_7G00204550 Rroxscaffold_7G00217350
rosa_rugosa Rorug01G0465500 Rorug06G0088500 Rorug06G0496700 Rorug06G0496700
rosa_samantha Rh1AG064800 Rh2BG015100 Rh2BG493500 Rh2DG017300 Rh2DG502500 Rh4BG208500 Rh5BG528500 Rh5CG552900 Rh6CG207800 Rh6DG197900 Rh6DG198000 Rh7AG101300 Rh7AG101400 Rh7AG101600 Rh7BG104100 Rh7BG104200 Rh7CG105600 Rh7CG105700 Rh7DG103200 Rh7DG103400
rosa_wichuraiana Rw1G007300 Rw5G034670 Rw7G008750 Rw7G008780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2024, 2151
AatII GACGTC 1 cut(s) 144
Acc16I TGCGCA 1 cut(s) 2876
Acc65I GGTACC 1 cut(s) 3942
AccB1I GGYRCC 1 cut(s) 3942
AccB7I CCANNNNNTGG 1 cut(s) 2072
AccBSI CCGCTC 1 cut(s) 1777
AccI GTMKAC 2 cut(s) 1991, 4243
AciI CCGC 7 cut(s) 568, 878, 960, 1777, 1901, 2243, 3298
AclWI GGATC 9 cut(s) 99, 197, 1295, 2292, 2305, 3524, 3844, 3969, 4431
AcoI YGGCCR 1 cut(s) 1804
AcuI CTGAAG 8 cut(s) 506, 720, 1742, 2576, 2588, 3657, 3861, 3917
AcyI GRCGYC 3 cut(s) 17, 141, 4046
AfeI AGCGCT 1 cut(s) 4107
AfiI CCNNNNNNNGG 4 cut(s) 85, 1052, 1886, 2072
AflIII ACRYGT 1 cut(s) 735
AhlI ACTAGT 1 cut(s) 1828
AjiI CACGTC 1 cut(s) 2082
AjnI CCWGG 4 cut(s) 99, 817, 1343, 2230
AjuI GAANNNNNNNTTGG 4 cut(s) 848, 880, 3946, 3978
AleI CACNNNNGTG 1 cut(s) 470
AloI GAACNNNNNNTCC 6 cut(s) 1136, 1168, 1251, 1283, 3525, 3557
Alw21I GWGCWC 1 cut(s) 30
Alw26I GTCTC 7 cut(s) 899, 2088, 2308, 2442, 3092, 3252, 4078
AlwI GGATC 9 cut(s) 99, 197, 1295, 2292, 2305, 3524, 3844, 3969, 4431
AlwNI CAGNNNCTG 1 cut(s) 3500
Ama87I CYCGRG 1 cut(s) 2927
Aor51HI AGCGCT 1 cut(s) 4107
AoxI GGCC 4 cut(s) 102, 1804, 2240, 3308
ApeKI GCWGC 9 cut(s) 1518, 2033, 2036, 2444, 2714, 2850, 3389, 4136, 4460
AseI ATTAAT 1 cut(s) 1184
Asp700I GAANNNNTTC 4 cut(s) 162, 2114, 2429, 4179
Asp718I GGTACC 1 cut(s) 3942
AspA2I CCTAGG 1 cut(s) 3092
AspLEI GCGC 4 cut(s) 1009, 2877, 3127, 4108
AspS9I GGNCC 1 cut(s) 3308
AsuC2I CCSGG 1 cut(s) 1604
AsuHPI GGTGA 7 cut(s) 89, 844, 1553, 2354, 2758, 2900, 3826
AsuNHI GCTAGC 1 cut(s) 1699
AvaI CYCGRG 1 cut(s) 2927
AvrII CCTAGG 1 cut(s) 3092
BaeGI GKGCMC 1 cut(s) 957
BaeI ACNNNNGTAYC 2 cut(s) 3884, 3917
BamHI GGATCC 1 cut(s) 2297
BanI GGYRCC 1 cut(s) 3942
BanII GRGCYC 2 cut(s) 256, 3582
BarI GAAGNNNNNNTAC 4 cut(s) 1107, 1139, 3152, 3184
BauI CACGAG 2 cut(s) 1167, 1575
BbsI GAAGAC 8 cut(s) 53, 183, 591, 1122, 1619, 2564, 2612, 3909
Bbv12I GWGCWC 1 cut(s) 30
BbvCI CCTCAGC 1 cut(s) 3948
BbvI GCAGC 9 cut(s) 1530, 2020, 2023, 2456, 2701, 2837, 3401, 4123, 4447
BceAI ACGGC 4 cut(s) 89, 258, 1791, 3090
BciT130I CCWGG 4 cut(s) 101, 819, 1345, 2232
BciVI GTATCC 1 cut(s) 2351
BclI TGATCA 4 cut(s) 300, 1705, 2632, 4449
BcnI CCSGG 1 cut(s) 1604
BcoDI GTCTC 7 cut(s) 899, 2088, 2308, 2442, 3092, 3252, 4078
BcuI ACTAGT 1 cut(s) 1828
BfaI CTAG 6 cut(s) 257, 1700, 1829, 2463, 3093, 4558
BfmI CTRYAG 3 cut(s) 246, 1498, 4237
BfoI RGCGCY 1 cut(s) 4109
BfuI GTATCC 1 cut(s) 2351
BglI GCCNNNNNGGC 1 cut(s) 1664
BlnI CCTAGG 1 cut(s) 3092
BlpI GCTNAGC 1 cut(s) 309
Bme1390I CCNGG 5 cut(s) 101, 819, 1345, 1604, 2232
BmeT110I CYCGRG 1 cut(s) 2927
BmgBI CACGTC 1 cut(s) 2082
BmgT120I GGNCC 1 cut(s) 3308
BmiI GGNNCC 6 cut(s) 597, 2299, 2545, 3492, 3525, 3944
BmrFI CCNGG 5 cut(s) 101, 819, 1345, 1604, 2232
BmrI ACTGGG 1 cut(s) 2245
BmtI GCTAGC 1 cut(s) 1703
BmuI ACTGGG 1 cut(s) 2245
BpiI GAAGAC 8 cut(s) 53, 183, 591, 1122, 1619, 2564, 2612, 3909
BplI GAGNNNNNCTC 2 cut(s) 57, 89
BpmI CTGGAG 2 cut(s) 3628, 4365
Bpu10I CCTNAGC 2 cut(s) 1713, 3948
Bpu1102I GCTNAGC 1 cut(s) 309
BpuEI CTTGAG 5 cut(s) 233, 965, 2033, 3493, 4371
BpuMI CCSGG 1 cut(s) 1604
Bsa29I ATCGAT 1 cut(s) 666
BsaAI YACGTR 1 cut(s) 3149
BsaBI GATNNNNATC 1 cut(s) 1734
BsaHI GRCGYC 3 cut(s) 17, 141, 4046
BsaI GGTCTC 1 cut(s) 3252
BsaWI WCCGGW 2 cut(s) 1996, 2661
BsaXI ACNNNNNCTCC 4 cut(s) 1136, 1166, 3525, 3555
Bsc4I CCNNNNNNNGG 4 cut(s) 85, 1052, 1886, 2072
Bse1I ACTGG 6 cut(s) 755, 1127, 1331, 2251, 3645, 3779
Bse3DI GCAATG 6 cut(s) 263, 367, 379, 2285, 2786, 3876
Bse8I GATNNNNATC 1 cut(s) 1734
BseBI CCWGG 4 cut(s) 101, 819, 1345, 2232
BseCI ATCGAT 1 cut(s) 666
BseJI GATNNNNATC 1 cut(s) 1734
BseLI CCNNNNNNNGG 4 cut(s) 85, 1052, 1886, 2072
BseMI GCAATG 6 cut(s) 263, 367, 379, 2285, 2786, 3876
BseMII CTCAG 9 cut(s) 323, 1664, 2183, 3384, 3962, 4152, 4340, 4389, 4434
BseNI ACTGG 6 cut(s) 755, 1127, 1331, 2251, 3645, 3779
BseRI GAGGAG 3 cut(s) 796, 4131, 4553
BseSI GKGCMC 1 cut(s) 957
BseXI GCAGC 9 cut(s) 1530, 2020, 2023, 2456, 2701, 2837, 3401, 4123, 4447
BsgI GTGCAG 1 cut(s) 2076
BshFI GGCC 4 cut(s) 104, 1806, 2242, 3310
BshNI GGYRCC 1 cut(s) 3942
BshVI ATCGAT 1 cut(s) 666
BsiHKAI GWGCWC 1 cut(s) 30
BsiHKCI CYCGRG 1 cut(s) 2927
BsiSI CCGG 4 cut(s) 120, 1603, 1997, 2662
BslFI GGGAC 4 cut(s) 2033, 2910, 3390, 4319
BslI CCNNNNNNNGG 4 cut(s) 85, 1052, 1886, 2072
BsmAI GTCTC 7 cut(s) 899, 2088, 2308, 2442, 3092, 3252, 4078
BsmBI CGTCTC 2 cut(s) 2088, 2308
BsmFI GGGAC 4 cut(s) 2033, 2910, 3390, 4319
BsmI GAATGC 7 cut(s) 175, 728, 2116, 2598, 2913, 3146, 3479
BsnI GGCC 4 cut(s) 104, 1806, 2242, 3310
Bso31I GGTCTC 1 cut(s) 3252
BsoBI CYCGRG 1 cut(s) 2927
Bsp1286I GDGCHC 4 cut(s) 30, 256, 957, 3582
Bsp1407I TGTACA 1 cut(s) 4030
Bsp1720I GCTNAGC 1 cut(s) 309
Bsp19I CCATGG 2 cut(s) 3215, 4146
BspACI CCGC 7 cut(s) 568, 878, 960, 1777, 1901, 2243, 3298
BspANI GGCC 4 cut(s) 104, 1806, 2242, 3310
BspDI ATCGAT 1 cut(s) 666
BspHI TCATGA 2 cut(s) 2635, 3195
BspLI GGNNCC 6 cut(s) 597, 2299, 2545, 3492, 3525, 3944
BspOI GCTAGC 1 cut(s) 1703
BspPI GGATC 9 cut(s) 99, 197, 1295, 2292, 2305, 3524, 3844, 3969, 4431
BspQI GCTCTTC 2 cut(s) 348, 3020
BspT107I GGYRCC 1 cut(s) 3942
BspTNI GGTCTC 1 cut(s) 3252
BsrBI CCGCTC 1 cut(s) 1777
BsrDI GCAATG 6 cut(s) 263, 367, 379, 2285, 2786, 3876
BsrGI TGTACA 1 cut(s) 4030
BsrI ACTGG 6 cut(s) 755, 1127, 1331, 2251, 3645, 3779
BssNAI GTATAC 1 cut(s) 1992
BssNI GRCGYC 3 cut(s) 17, 141, 4046
BssSI CACGAG 2 cut(s) 1167, 1575
Bst1107I GTATAC 1 cut(s) 1992
Bst2BI CACGAG 2 cut(s) 1167, 1575
Bst2UI CCWGG 4 cut(s) 101, 819, 1345, 2232
Bst6I CTCTTC 6 cut(s) 348, 817, 3020, 4035, 4160, 4349
BstACI GRCGYC 3 cut(s) 17, 141, 4046
BstAUI TGTACA 1 cut(s) 4030
BstBAI YACGTR 1 cut(s) 3149
BstDSI CCRYGG 2 cut(s) 3215, 4146
BstEII GGTNACC 1 cut(s) 850
BstH2I RGCGCY 1 cut(s) 4109
BstHHI GCGC 4 cut(s) 1009, 2877, 3127, 4108
BstMAI GTCTC 7 cut(s) 899, 2088, 2308, 2442, 3092, 3252, 4078
BstNI CCWGG 4 cut(s) 101, 819, 1345, 2232
BstNSI RCATGY 6 cut(s) 739, 1671, 1943, 2033, 2412, 3737
BstPI GGTNACC 1 cut(s) 850
BstSCI CCNGG 5 cut(s) 99, 817, 1343, 1602, 2230
BstSFI CTRYAG 3 cut(s) 246, 1498, 4237
BstSLI GKGCMC 1 cut(s) 957
BstSNI TACGTA 1 cut(s) 3149
BstV1I GCAGC 9 cut(s) 1530, 2020, 2023, 2456, 2701, 2837, 3401, 4123, 4447
BstV2I GAAGAC 8 cut(s) 53, 183, 591, 1122, 1619, 2564, 2612, 3909
BstX2I RGATCY 5 cut(s) 91, 2297, 3516, 3836, 3974
BstXI CCANNNNNNTGG 3 cut(s) 268, 847, 4153
BstYI RGATCY 5 cut(s) 91, 2297, 3516, 3836, 3974
BstZ17I GTATAC 1 cut(s) 1992
Bsu15I ATCGAT 1 cut(s) 666
BsuI GTATCC 1 cut(s) 2351
BsuRI GGCC 4 cut(s) 104, 1806, 2242, 3310
BsuTUI ATCGAT 1 cut(s) 666
BtgI CCRYGG 2 cut(s) 3215, 4146
BtrI CACGTC 1 cut(s) 2082
BtsI GCAGTG 1 cut(s) 303
BtsIMutI CAGTG 8 cut(s) 303, 355, 477, 609, 1338, 3498, 3652, 3772
CaiI CAGNNNCTG 1 cut(s) 3500
CciI TCATGA 2 cut(s) 2635, 3195
CfoI GCGC 4 cut(s) 1009, 2877, 3127, 4108
Cfr13I GGNCC 1 cut(s) 3308
ClaI ATCGAT 1 cut(s) 666
CseI GACGC 3 cut(s) 25, 3739, 4035
CspCI CAANNNNNGTGG 2 cut(s) 946, 981
EaeI YGGCCR 1 cut(s) 1804
Eam1104I CTCTTC 6 cut(s) 348, 817, 3020, 4035, 4160, 4349
EarI CTCTTC 6 cut(s) 348, 817, 3020, 4035, 4160, 4349
EciI GGCGGA 1 cut(s) 893
Eco105I TACGTA 1 cut(s) 3149
Eco24I GRGCYC 2 cut(s) 256, 3582
Eco31I GGTCTC 1 cut(s) 3252
Eco47III AGCGCT 1 cut(s) 4107
Eco57I CTGAAG 8 cut(s) 506, 720, 1742, 2576, 2588, 3657, 3861, 3917
Eco88I CYCGRG 1 cut(s) 2927
Eco91I GGTNACC 1 cut(s) 850
EcoO65I GGTNACC 1 cut(s) 850
EcoRI GAATTC 5 cut(s) 767, 1762, 2639, 3326, 3778
EcoRII CCWGG 4 cut(s) 99, 817, 1343, 2230
EcoT22I ATGCAT 5 cut(s) 832, 1361, 2536, 2752, 3146
EcoT38I GRGCYC 2 cut(s) 256, 3582
Esp3I CGTCTC 2 cut(s) 2088, 2308
FalI AAGNNNNNCTT 8 cut(s) 796, 828, 927, 959, 3269, 3301, 3946, 3978
FaqI GGGAC 4 cut(s) 2033, 2910, 3390, 4319
FauI CCCGC 1 cut(s) 1894
FauNDI CATATG 1 cut(s) 1795
FbaI TGATCA 4 cut(s) 300, 1705, 2632, 4449
FblI GTMKAC 2 cut(s) 1991, 4243
FriOI GRGCYC 2 cut(s) 256, 3582
FspBI CTAG 6 cut(s) 257, 1700, 1829, 2463, 3093, 4558
FspI TGCGCA 1 cut(s) 2876
GlaI GCGC 4 cut(s) 1008, 2876, 3126, 4107
GsuI CTGGAG 2 cut(s) 3628, 4365
HaeII RGCGCY 1 cut(s) 4109
HaeIII GGCC 4 cut(s) 104, 1806, 2242, 3310
HapII CCGG 4 cut(s) 120, 1603, 1997, 2662
HgaI GACGC 3 cut(s) 25, 3739, 4035
HhaI GCGC 4 cut(s) 1009, 2877, 3127, 4108
Hin1I GRCGYC 3 cut(s) 17, 141, 4046
Hin6I GCGC 4 cut(s) 1007, 2875, 3125, 4106
HinP1I GCGC 4 cut(s) 1007, 2875, 3125, 4106
HincII GTYRAC 3 cut(s) 1111, 2141, 2311
HindII GTYRAC 3 cut(s) 1111, 2141, 2311
HindIII AAGCTT 4 cut(s) 1234, 1244, 1781, 2006
HpaII CCGG 4 cut(s) 120, 1603, 1997, 2662
HphI GGTGA 7 cut(s) 89, 844, 1553, 2354, 2758, 2900, 3826
Hpy166II GTNNAC 9 cut(s) 145, 365, 1111, 1405, 1992, 2141, 2311, 3590, 4244
Hpy8I GTNNAC 9 cut(s) 145, 365, 1111, 1405, 1992, 2141, 2311, 3590, 4244
Hpy99I CGWCG 3 cut(s) 10, 13, 3047
HpyCH4IV ACGT 4 cut(s) 141, 2081, 3148, 4294
HpySE526I ACGT 4 cut(s) 141, 2081, 3148, 4294
Hsp92I GRCGYC 3 cut(s) 17, 141, 4046
HspAI GCGC 4 cut(s) 1007, 2875, 3125, 4106
KpnI GGTACC 1 cut(s) 3946
Ksp22I TGATCA 4 cut(s) 300, 1705, 2632, 4449
LguI GCTCTTC 2 cut(s) 348, 3020
LmnI GCTCC 7 cut(s) 25, 1144, 1954, 2543, 2649, 2847, 4144
Lsp1109I GCAGC 9 cut(s) 1530, 2020, 2023, 2456, 2701, 2837, 3401, 4123, 4447
MaeI CTAG 6 cut(s) 257, 1700, 1829, 2463, 3093, 4558
MaeII ACGT 4 cut(s) 141, 2081, 3148, 4294
MbiI CCGCTC 1 cut(s) 1777
MfeI CAATTG 4 cut(s) 621, 1435, 2379, 3593
MflI RGATCY 5 cut(s) 91, 2297, 3516, 3836, 3974
MhlI GDGCHC 4 cut(s) 30, 256, 957, 3582
MlyI GAGTC 5 cut(s) 1097, 1517, 1615, 3220, 3266
Mph1103I ATGCAT 5 cut(s) 832, 1361, 2536, 2752, 3146
MroXI GAANNNNTTC 4 cut(s) 162, 2114, 2429, 4179
MslI CAYNNNNRTG 8 cut(s) 372, 470, 845, 1463, 2483, 3426, 3484, 4151
MspA1I CMGCKG 3 cut(s) 698, 3392, 4217
MspI CCGG 4 cut(s) 120, 1603, 1997, 2662
MspR9I CCNGG 5 cut(s) 101, 819, 1345, 1604, 2232
MunI CAATTG 4 cut(s) 621, 1435, 2379, 3593
Mva1269I GAATGC 7 cut(s) 175, 728, 2116, 2598, 2913, 3146, 3479
MvaI CCWGG 4 cut(s) 101, 819, 1345, 2232
NciI CCSGG 1 cut(s) 1604
NcoI CCATGG 2 cut(s) 3215, 4146
NdeI CATATG 1 cut(s) 1795
NheI GCTAGC 1 cut(s) 1699
NlaIV GGNNCC 6 cut(s) 597, 2299, 2545, 3492, 3525, 3944
NmuCI GTSAC 5 cut(s) 850, 1454, 1618, 2411, 4047
NsbI TGCGCA 1 cut(s) 2876
NsiI ATGCAT 5 cut(s) 832, 1361, 2536, 2752, 3146
NspI RCATGY 6 cut(s) 739, 1671, 1943, 2033, 2412, 3737
OliI CACNNNNGTG 1 cut(s) 470
PaeI GCATGC 2 cut(s) 1671, 3737
PagI TCATGA 2 cut(s) 2635, 3195
PciI ACATGT 1 cut(s) 735
PciSI GCTCTTC 2 cut(s) 348, 3020
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PctI GAATGC 7 cut(s) 175, 728, 2116, 2598, 2913, 3146, 3479
PdmI GAANNNNTTC 4 cut(s) 162, 2114, 2429, 4179
PflMI CCANNNNNTGG 1 cut(s) 2072
PleI GAGTC 5 cut(s) 1096, 1516, 1615, 3220, 3265
PpsI GAGTC 5 cut(s) 1096, 1516, 1615, 3220, 3265
Ppu21I YACGTR 1 cut(s) 3149
PscI ACATGT 1 cut(s) 735
PshBI ATTAAT 1 cut(s) 1184
PsiI TTATAA 2 cut(s) 2024, 2151
Psp6I CCWGG 4 cut(s) 99, 817, 1343, 2230
PspEI GGTNACC 1 cut(s) 850
PspGI CCWGG 4 cut(s) 99, 817, 1343, 2230
PspN4I GGNNCC 6 cut(s) 597, 2299, 2545, 3492, 3525, 3944
PspPI GGNCC 1 cut(s) 3308
PstNI CAGNNNCTG 1 cut(s) 3500
PsuI RGATCY 5 cut(s) 91, 2297, 3516, 3836, 3974
PvuII CAGCTG 3 cut(s) 698, 3392, 4217
RseI CAYNNNNRTG 8 cut(s) 372, 470, 845, 1463, 2483, 3426, 3484, 4151
SapI GCTCTTC 2 cut(s) 348, 3020
Sau96I GGNCC 1 cut(s) 3308
SchI GAGTC 5 cut(s) 1097, 1517, 1615, 3220, 3266
ScrFI CCNGG 5 cut(s) 101, 819, 1345, 1604, 2232
SduI GDGCHC 4 cut(s) 30, 256, 957, 3582
SfcI CTRYAG 3 cut(s) 246, 1498, 4237
SmiMI CAYNNNNRTG 8 cut(s) 372, 470, 845, 1463, 2483, 3426, 3484, 4151
SmlI CTYRAG 5 cut(s) 212, 944, 2012, 3472, 4386
SmoI CTYRAG 5 cut(s) 212, 944, 2012, 3472, 4386
SnaBI TACGTA 1 cut(s) 3149
SpeI ACTAGT 1 cut(s) 1828
SphI GCATGC 2 cut(s) 1671, 3737
SsiI CCGC 7 cut(s) 568, 878, 960, 1777, 1901, 2243, 3298
SspMI CTAG 6 cut(s) 257, 1700, 1829, 2463, 3093, 4558
StyD4I CCNGG 5 cut(s) 99, 817, 1343, 1602, 2230
TaiI ACGT 4 cut(s) 144, 2084, 3151, 4297
TaqI TCGA 8 cut(s) 666, 1562, 2731, 3045, 3156, 3718, 3767, 3997
TatI WGTACW 3 cut(s) 1404, 3009, 4030
TauI GCSGC 2 cut(s) 571, 2245
TscAI CASTG 8 cut(s) 310, 355, 477, 616, 1338, 3505, 3652, 3779
TseFI GTSAC 5 cut(s) 850, 1454, 1618, 2411, 4047
TseI GCWGC 9 cut(s) 1518, 2033, 2036, 2444, 2714, 2850, 3389, 4136, 4460
Tsp45I GTSAC 5 cut(s) 850, 1454, 1618, 2411, 4047
TspGWI ACGGA 5 cut(s) 58, 101, 2290, 3637, 4436
TspRI CASTG 8 cut(s) 310, 355, 477, 616, 1338, 3505, 3652, 3779
Van91I CCANNNNNTGG 1 cut(s) 2072
VspI ATTAAT 1 cut(s) 1184
XceI RCATGY 6 cut(s) 739, 1671, 1943, 2033, 2412, 3737
XcmI CCANNNNNNNNNTGG 1 cut(s) 3534
XmaJI CCTAGG 1 cut(s) 3092
XmiI GTMKAC 2 cut(s) 1991, 4243
XmnI GAANNNNTTC 4 cut(s) 162, 2114, 2429, 4179
XspI CTAG 6 cut(s) 257, 1700, 1829, 2463, 3093, 4558
ZraI GACGTC 1 cut(s) 142
Zsp2I ATGCAT 5 cut(s) 832, 1361, 2536, 2752, 3146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.