Rroxscaffold_3G00265640

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000526
Physical Location & Seq
Forward (+)
59059016 .. 59066573
7558 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00265640.1

Sequence Viewer

Length: 5379 bp
ATGGCGGCGACGATGACGCCAAGGGAGCACATAGAAGATATACGGAAGACCAAGTTCTCCATCGGAGAAAAGCTCAACCCTTTAACGGAGGATCTTCACCAGGCCGTCAAGAACCTCTCCGGCGAGCTTTACGCTAAAGACGTCCACTTTTTCATGGAACTCGTTCAGAATGCAGAAGACAATGAGTACTTGGAGGGGTTGATCCATCCCTTGAGAATTGGCTTCAAGAGTGTGTTTCTGATCACTGCTCAGCCTTACATATTTAGTAATGGTTATCAGATAAGGTTTAGTGAAGAGCCTTGTCCACATTGCAATGTTGGCTACATAGTACCTGAATGGATTGAGCAGAATCCCACTCTATCTGATATAAAAAAGATATATGGTTCTGCTACTGCGCTTCCTACTACAACACTGATCTTACCTCTGAAGCCGGACAAGGTTGAAGCTGTGAAGAAACAACTCTCAATCATTCATCCAGAAGTTCTCTTGTTCCTATCAAAGATTAAGCGGCTCTCTGTTAGGGAAGACAAACAGGACCCAAGGCAGAACACTGTGAGTGCAATTGAGATAGCAAGCGAGACTGCACATGCGTCAAGGAAGAACATCGACGCTCAATCCTACACTCTGCATCTTTCAGCTGAAGAAAATGATGGAAATGGAGAATGCAGTTACTACATGTGGAAACAAAAGTTTCCTGTCAGGGAGGAATTCAGAGTTGAGAGGAGAATGGAGGTGGATGAGTGGGTGATCACTCTTGCATTTCCAATTGGAGAACGTCTTCGAAGAGCAAGGAACTCTTCGCCTGGTGTTTATGCATTTCTTCCCACAGAGATGGTCACCAACTTTCCATTCATAATACAGGCGGATTTTCTTTTGGCATCTTCAAGAGAGACAATCCTACTTGACAACAAGTGGAATCAAGGGATTCTTGAGTGTGTGCCCACTGCATTTGCCCATGCCTTTAACTCTCTTGTGAAAACTGTGGAAGATGCGCCAGCATCAAATCTGGCTCCTATGTTCAGATTCTTGCCTGTTCAGGGTTCTTCCTATAAAGAATTAAATGCTGTAAGGGAGTCAATAAAAGCAAAGATGGTTAACGAAGACATTATACCAGTTGAGCCTGAAATGGAGCAGAAGTTCTTCCACAAGCCTCGTGAAGTGGGAAGATTGATGCCTGCCTTTTGGACTATTTTGAGGAAGGCGATGGATCAAGGTGTAAGCTTGATTAAGCTTTCATCACATGGAAGATTTGTTCTGTCTTATTCCTTTGACAGCGAGGAATATGATCCTGTACTAAGTTTCCTTGGAGTTGAACCAGTGGATGATGATTGGTATGTTAAATGCATACAGGGTACTTCTAATCTTGTTGATGGAGTGTCAGAAGATGTGTACTTAGAGCTTCTACTGTTTATCGCTGACAATTGGGGTTCTAAATTTGGTCACACCAACATGAAGAACATACCATTGATAAAATATGAAGACTATGGAAATGAATCTTTGTGCAGCATAAGAGAAATCCAGAAAGGTGAATTTAGAGTTTGCTTATCGAGCAAATATACTCATGTTTCATGGCTGATTGATTGCAACCGGGAATTTAATTCTGTCACTTGTCTTCTCTTTATGCCAAAAGCCACTCAGGAAGCCATCTGGGCAAGCTCTAGGAAGGATACACTGAAGAAATGGCTTGCTGATCAAGCTAAGGTTGGTTCTGTTGATGTCAATCAATATGCAGATGATCTCCTTCAGAATTCTCTTAATGAGCGGAAGCTTGTTATTGCTTATGTTCACTTCTTATATCAGTCATTCAACAATGATTTTCTCTCTAGTGGGAAAGTTGATAATATATGTCGAAGTATGCCACTAGTGGATAGCTATGGAAACATCATCAGGATTCGAAAAGGAGTTGTTGTTCCTGCCAATGGAAGCAAATGGGCGGGATTGACTGATTCTAATTTGTGGAGAAAAGAAGGCTATGTTGAATTAGGAGAGCATTATATGTATTCTGGCAATTTTGCTGGTAAGAATACACCGGAGAAGATGCTTCTTGAGTTCCTTACAGAACATGCTGCTGCTTTGGATGTCCCTCATATTTCTGCTCCAAGTGATGGTATCTCGTCTGTATCTGCACCACTAACTAAGCAGAATGCCTTCTTGCTTTTGGATTGGATTCGTCAACTGAATTATAAAAGGGTTGGCATCCCTCAGAAGTTCTTGGCTTGCATAAAGGAAGGTAGTTGGCTGAAAGTTACTTTGAATGGCTCTCCTGGTTATAGGCCACCATCCCAGTCATTTCTGCTTAACTCATCATGGGGAAATACTTTGCAGAATGGATCCGTCTCTGTTGACATTCCATTGATTGATACGAGCTTTTATGGTGAGAGCATTAGTGAATATAAGTGGGAGCTAAAGACAATTGGGGTCATGTTTGAATATAGCGAGGCATGTGACTTCATTGGGAAGCATTTCATGTCTCTGGCAGCTTCATCCTCTTTAACTAGAGGAAATGTGCATTCCATTCTACGTTTCATCAGATTTTTAAGGGACAAACTTCTTCCTCCGGCCGATTTTATCTCTGCTATTAAGAAAGGGAACTGGCTGAAGACTACCCTTGGCTACAGGTCTCCTGGAGGATCTGTTTTGTCAGATAAGGAGTGGAATGTTGCCTCCAAAATTTGCAACATCCCCTTCATTGATCAGGACTTTTACGGTGATGAAATCTGTAATTTCAGAACTGAACTTGAGTTGCTCGGTGTTGTAACCTCCTTCGACGGAAGTTACCAACTTATAATTGACAACCTGAAGTCACCATCTTCCATAACTTCTTGGACACCTCAGGTTGTCATCTTAATGCTCAACTGTATAGCTCTATCCAAATCATCTGATAAACTTGTCAATGCACTGAAAGGAGCCAAGTGCCTGAAGACAAGTATTGGTTACAAGTGTCCAGGGGAATGTCTCTTGTTTCATGACGAATGGGGTTGCATTCTCCAGGTCGTCAATGGTCTTCCTGTGATTGATCATGAATTCTACAGGAGCAACATTTTTCGGTACAGAAATGAACTGAAAAAAATTGGTGTGGTGGTTGATTTTGAAGAGGCTGTAAAAGTATTTGCTCGATATTTCAGGCGCTATGCATTATCAGCTTCCATCACCAAGGAAAATGCTGCATCATTGCTTCTATGTTACAGAGCGCTGAATGGGACTCCATATAAATTTCCTGCTGATCTTAGGAGCTGCATTGGTGAGGTCAAGTGGTTGCGTAGTCGTTTTGGTGATTACAGATGTCCAAGGGAGTGCATTCTATTTAGTCCTGAGTGGGAATCTATATCCCCAATAACTCTGCTTCCTTTCATTGATGATTCTGACAGTTGCTATGGAAATAGCATACATGAGTACAGAAAAGAGTTGAAGAGCTTGGGGGTTGTCGTCAGATACAAGGAAGGAGTCAAGTTTGTGGCTTCTGGTCTTTATCTCCCTAGGGATTCCAGCCGTATTTTTCCTGAAAATGCACTTGCGTTGCTGGAATGCATACGAGTTTTCCAGGAGAAAGGTTACGCCTTTCTTGATGATTGTGATTTCGTGAAGAAAATTTCTCAACCATGGATAAAGACTCACGCTGGTTATAGGTCACCAAAAGATTCCTTGTTGTTTGATTTCAAGTTTGGCTCATATTTGAAGCCGACTGATGGGCCTTTCATTGATGAAAAATTTTATGGTACTAAGATTGCAACGTACAAAAAAGAGCTCTCTGCATTGGGGGTTATTGTTGAAGCTGAAAAAGGATGTCCCTTGATTGCCAGCCACCTTGATGTACATAATGAGATTGAAGCTTTTGTTCGATTGTATAGTTACTTGAGTGCGTTCAAGTGGGAGCCAGACACTGATGCTGCTCAAAGGATCTGGATTCCGAAAGGGGATTGGGTTTGTCCTGATGAATGTGTTATTTATGACAAGGATGGGCTATTTGGTTTGCAGTTGACTGTTTTGGAGAAACATTATGAGCATGAACTTCTGTCTTTCTTCTCCAGTGCTTTTAGAGTCAAATACAATCCTACAGTTGATGACTACTTGAAGGTTTGGAAGGTTTGGGAAAGTTCTGGAAGTGCATTGTCGCGTGCTGATTGTTGCAAGTTTTGGAGTTATATCTCGAAGCACTGGAATTCAGAGACAGAAAAGACTGTTGCTGATGCCTTGGTGAAAGTACCTGTCAGTTCTGGATCTGAAGGAATCTTACTGTGCAACAAGTCTGATGTTTTCATTGCTGATGACCTTCAACTGAAGTATTTATTCGAACAGTCTTCTCCTGGGTCAATTTTTGTTTGGTACCCTCAGCCAAGTTTGCCTTCCATTCCAAGATCCAAGTTGCTTGACATTTATCGAAGAATTGGTGTTCGCACTATATCTGAATCTGTACAGAAGGCAGAGGCGTCACTTGCAGATGATGTTGAAGTAGTCTCTTTGCCGAGGGAAAAGCTGATTAAGAAAGCGCTGTTCAGGCTGATTCTTGGTTTTCTTGCTGCTCCTCCCATGGAAATGGAAGCTGAGAAGAGGCTTGAAGCTGTTCAATTCCTTGAGAATGTTACTGTGGTGGTAACAGCTGAACCAATCTCTGTAAGCTATAATCTACCTTTATCTTCTGGGAAAGTTTTGAATGTGAGAGGAAGCCGAAAAATACGGTTTGATAGAGAAAATTCAAAGATCTTCACTCAGAAAATGGACAAGTCTGGAGGGCAGATGAGTATCATTGTGTTTGCTACTTTTTTCTCGGAAGCAATATCTGAGAGTGTGTTATGGGAGAGCACTGATCATATAGATTCACTCTCTGAGCTGATCAAGATGGCTTCTTTGCTAGACTTCAATGAGGAAGCAGTTGATTTCTTGATGAAGTCCAAGAATTTGCAAACCTTTATGGAGGATGAGGAGTTTCTCAAGACTGATTGTTTGTTAGAATCCTTTGATTTGTTGTGTGCATATGATTTGTTGCGTTTGATTGAGTTTGCCACTTCTTTCTCTCAAGCAATATCTGAGAGTGTGTTATGGGATAGTACGGATCATATAGATTCACTCTCTGAGCTGATCAAGGTGGCTGCTTTGCTAAACTTCAATGAGGAAGCAGTTGATTTCTTGATGAAGTCCAAGAATTTGCAAATCTTCATGGAGGATGAGGAGTTTCTCAGGACTGTCTACCCTCTTGGGCAGTTATGTGGTTACAATACTATGGATCCTGATGGTCTATCAGTTGATTCTTTGAAGTTTCATTCTGCTCCCACTGTCATTCTTCCTCTCTGTGAAGCTAATCTGTGCATTGATGATTTGTTAGCATTAAGCAGCTCATCAGATGATGAATCAAACAGACCAGATATCCATTCATCTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1792

Amino Acids

203.2

Weight (kDa)

5.48

Isoelectric Point (pI)

40.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28020 AT3G48770 AT3G48770
fragaria_vesca FvH4_5g09020 FvH4_5g09020
malus_domestica MD06G1092400.v1.1 MD06G1134600.v1.1 MD06G1134700.v1.1 MD06G1134800.v1.1 MD06G1135200.v1.1 MD06G1135400.v1.1 MD06G1135500.v1.1 MD08G1137600.v1.1 MD15G1395100.v1.1
prunus_persica Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145600_v2.0.a1 Prupe.5G145800_v2.0.a1 Prupe.5G145900_v2.0.a1
pyrus_communis pycom06g12550 pycom06g12570 pycom06g12580 pycom06g12590 pycom06g12610 pycom06g12630 pycom06g12640 pycom06g12650 pycom08g06280
rosa_chinensis RchiOBHm_Chr6g0249321 RchiOBHm_Chr6g0275111 RchiOBHm_Chr7g0189191 RchiOBHm_Chr7g0189261
rosa_laevigata RLG00000004622 RLG00000004623 RLG00000008237 RLG00000008238 RLG00000023303 RLG00000035187
rosa_multiflora Rmu_co8294311.1_g000001 Rmu_co8389833.1_g000001 Rmu_sc0001044.1_g000004 Rmu_sc0001329.1_g000011 Rmu_sc0006012.1_g000010 Rmu_sc0007286.1_g000032 Rmu_sc0009360.1_g000016 Rmu_sc0011817.1_g000001 Rmu_sc0015050.1_g000001
rosa_roxburghii Rroxscaffold_1G00064000 Rroxscaffold_3G00265460 Rroxscaffold_3G00265580 Rroxscaffold_3G00265640 Rroxscaffold_3G00265690 Rroxscaffold_3G00265700 Rroxscaffold_7G00193620 Rroxscaffold_7G00204550 Rroxscaffold_7G00217350
rosa_rugosa Rorug01G0465500 Rorug06G0088500 Rorug06G0496700 Rorug06G0496700
rosa_samantha Rh1AG064800 Rh2BG015100 Rh2BG493500 Rh2DG017300 Rh2DG502500 Rh4BG208500 Rh5BG528500 Rh5CG552900 Rh6CG207800 Rh6DG197900 Rh6DG198000 Rh7AG101300 Rh7AG101400 Rh7AG101600 Rh7BG104100 Rh7BG104200 Rh7CG105600 Rh7CG105700 Rh7DG103200 Rh7DG103400
rosa_wichuraiana Rw1G007300 Rw5G034670 Rw7G008750 Rw7G008780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2184, 2786
AatII GACGTC 1 cut(s) 144
Acc65I GGTACC 1 cut(s) 4323
AccB1I GGYRCC 1 cut(s) 4323
AccB7I CCANNNNNTGG 1 cut(s) 2105
AccBSI CCGCTC 1 cut(s) 1762
AccI GTMKAC 1 cut(s) 5184
AccII CGCG 1 cut(s) 4114
AciI CCGC 5 cut(s) 5, 508, 863, 1762, 1934
AcoI YGGCCR 1 cut(s) 2559
AcuI CTGAAG 9 cut(s) 446, 660, 1694, 1727, 2618, 2819, 2939, 4242, 4298
AcyI GRCGYC 3 cut(s) 17, 141, 4427
AfeI AGCGCT 2 cut(s) 3192, 4488
AfiI CCNNNNNNNGG 5 cut(s) 85, 1037, 1919, 2105, 3686
AflIII ACRYGT 1 cut(s) 675
AhlI ACTAGT 1 cut(s) 1861
AjnI CCWGG 8 cut(s) 99, 802, 2263, 2623, 2944, 2988, 3540, 4303
AjuI GAANNNNNNNTTGG 4 cut(s) 833, 865, 4327, 4359
AloI GAACNNNNNNTCC 6 cut(s) 1121, 1153, 1236, 1268, 1893, 1925
Alw21I GWGCWC 3 cut(s) 30, 3747, 4802
Alw26I GTCTC 8 cut(s) 572, 884, 2341, 2475, 2625, 2960, 4160, 4459
AlwNI CAGNNNCTG 1 cut(s) 3881
Aor51HI AGCGCT 2 cut(s) 3192, 4488
AoxI GGCC 4 cut(s) 102, 2273, 2559, 3689
ArsI GACNNNNNNTTYG 2 cut(s) 4924, 4956
Asp700I GAANNNNTTC 6 cut(s) 162, 777, 1139, 2147, 2462, 4560
Asp718I GGTACC 1 cut(s) 4323
AspA2I CCTAGG 1 cut(s) 3476
AspLEI GCGC 5 cut(s) 397, 994, 3129, 3193, 4489
AspS9I GGNCC 2 cut(s) 535, 3689
AsuC2I CCSGG 1 cut(s) 1589
AsuII TTCGAA 3 cut(s) 781, 1894, 4290
AvaII GGWCC 1 cut(s) 535
AvrII CCTAGG 1 cut(s) 3476
AxyI CCTNAGG 1 cut(s) 2832
BaeGI GKGCMC 1 cut(s) 942
BamHI GGATCC 2 cut(s) 2330, 5221
BanI GGYRCC 1 cut(s) 4323
BanII GRGCYC 1 cut(s) 3747
BarI GAAGNNNNNNTAC 4 cut(s) 1092, 1124, 4215, 4247
BauI CACGAG 1 cut(s) 1152
Bbv12I GWGCWC 3 cut(s) 30, 3747, 4802
BbvCI CCTCAGC 1 cut(s) 4329
BceAI ACGGC 2 cut(s) 89, 3474
BciT130I CCWGG 8 cut(s) 101, 804, 2265, 2625, 2946, 2990, 3542, 4305
BciVI GTATCC 1 cut(s) 1660
BclI TGATCA 8 cut(s) 240, 747, 1690, 2692, 3016, 4804, 4830, 5076
BcnI CCSGG 1 cut(s) 1589
BcoDI GTCTC 8 cut(s) 572, 884, 2341, 2475, 2625, 2960, 4160, 4459
BcuI ACTAGT 1 cut(s) 1861
BfaI CTAG 6 cut(s) 1659, 1824, 1862, 2496, 3477, 4850
BfmI CTRYAG 3 cut(s) 2614, 3028, 4053
BfoI RGCGCY 3 cut(s) 3130, 3194, 4490
BfuI GTATCC 1 cut(s) 1660
BglI GCCNNNNNGGC 1 cut(s) 1649
BglII AGATCT 1 cut(s) 4698
BlnI CCTAGG 1 cut(s) 3476
BlpI GCTNAGC 1 cut(s) 249
BmcAI AGTACT 1 cut(s) 188
Bme1390I CCNGG 9 cut(s) 101, 804, 1589, 2265, 2625, 2946, 2990, 3542, 4305
Bme18I GGWCC 1 cut(s) 535
BmgT120I GGNCC 2 cut(s) 535, 3689
BmiI GGNNCC 7 cut(s) 537, 1011, 2332, 2908, 3873, 4325, 5223
BmrFI CCNGG 9 cut(s) 101, 804, 1589, 2265, 2625, 2946, 2990, 3542, 4305
BmrI ACTGGG 1 cut(s) 2278
BmuI ACTGGG 1 cut(s) 2278
BplI GAGNNNNNCTC 2 cut(s) 57, 89
BpmI CTGGAG 4 cut(s) 2646, 2972, 4009, 4746
Bpu10I CCTNAGC 2 cut(s) 1698, 4329
Bpu1102I GCTNAGC 1 cut(s) 249
Bpu14I TTCGAA 3 cut(s) 781, 1894, 4290
BpuEI CTTGAG 8 cut(s) 232, 950, 2066, 2759, 3874, 4592, 4913, 4998
BpuMI CCSGG 1 cut(s) 1589
BsaBI GATNNNNATC 2 cut(s) 1719, 4739
BsaHI GRCGYC 3 cut(s) 17, 141, 4427
BsaI GGTCTC 1 cut(s) 2625
BsaWI WCCGGW 1 cut(s) 2029
BsaXI ACNNNNNCTCC 6 cut(s) 1121, 1151, 1893, 1923, 2619, 2649
Bsc4I CCNNNNNNNGG 5 cut(s) 85, 1037, 1919, 2105, 3686
Bse1I ACTGG 6 cut(s) 1112, 1316, 2284, 2597, 4026, 4160
Bse21I CCTNAGG 1 cut(s) 2832
Bse3DI GCAATG 4 cut(s) 307, 319, 3170, 4257
Bse8I GATNNNNATC 2 cut(s) 1719, 4739
BseBI CCWGG 8 cut(s) 101, 804, 2265, 2625, 2946, 2990, 3542, 4305
BseJI GATNNNNATC 2 cut(s) 1719, 4739
BseLI CCNNNNNNNGG 5 cut(s) 85, 1037, 1919, 2105, 3686
BseMI GCAATG 4 cut(s) 307, 319, 3170, 4257
BseNI ACTGG 6 cut(s) 1112, 1316, 2284, 2597, 4026, 4160
BseRI GAGGAG 4 cut(s) 736, 4512, 4934, 5180
BseSI GKGCMC 1 cut(s) 942
BseX3I CGGCCG 1 cut(s) 2559
BsgI GTGCAG 3 cut(s) 567, 1522, 2109
Bsh1236I CGCG 1 cut(s) 4114
Bsh1285I CGRYCG 1 cut(s) 2562
BshFI GGCC 4 cut(s) 104, 2275, 2561, 3691
BshNI GGYRCC 1 cut(s) 4323
BsiEI CGRYCG 1 cut(s) 2562
BsiHKAI GWGCWC 3 cut(s) 30, 3747, 4802
BsiSI CCGG 5 cut(s) 120, 431, 1588, 2030, 2558
BslFI GGGAC 4 cut(s) 2066, 2555, 3214, 3771
BslI CCNNNNNNNGG 5 cut(s) 85, 1037, 1919, 2105, 3686
BsmAI GTCTC 8 cut(s) 572, 884, 2341, 2475, 2625, 2960, 4160, 4459
BsmBI CGTCTC 1 cut(s) 2341
BsmFI GGGAC 4 cut(s) 2066, 2555, 3214, 3771
BsmI GAATGC 7 cut(s) 175, 668, 2149, 2509, 2982, 3297, 3530
BsnI GGCC 4 cut(s) 104, 2275, 2561, 3691
Bso31I GGTCTC 1 cut(s) 2625
Bsp119I TTCGAA 3 cut(s) 781, 1894, 4290
Bsp1286I GDGCHC 4 cut(s) 30, 942, 3747, 4802
Bsp1407I TGTACA 2 cut(s) 3811, 4411
Bsp1720I GCTNAGC 1 cut(s) 249
Bsp19I CCATGG 2 cut(s) 3599, 4527
BspACI CCGC 5 cut(s) 5, 508, 863, 1762, 1934
BspANI GGCC 4 cut(s) 104, 2275, 2561, 3691
BspFNI CGCG 1 cut(s) 4114
BspHI TCATGA 2 cut(s) 2965, 3019
BspLI GGNNCC 7 cut(s) 537, 1011, 2332, 2908, 3873, 4325, 5223
BspQI GCTCTTC 3 cut(s) 288, 778, 3404
BspT104I TTCGAA 3 cut(s) 781, 1894, 4290
BspT107I GGYRCC 1 cut(s) 4323
BspTNI GGTCTC 1 cut(s) 2625
BsrBI CCGCTC 1 cut(s) 1762
BsrDI GCAATG 4 cut(s) 307, 319, 3170, 4257
BsrGI TGTACA 2 cut(s) 3811, 4411
BsrI ACTGG 6 cut(s) 1112, 1316, 2284, 2597, 4026, 4160
BssNI GRCGYC 3 cut(s) 17, 141, 4427
BssSI CACGAG 1 cut(s) 1152
Bst2BI CACGAG 1 cut(s) 1152
Bst2UI CCWGG 8 cut(s) 101, 804, 2265, 2625, 2946, 2990, 3542, 4305
Bst6I CTCTTC 6 cut(s) 288, 778, 802, 3087, 3404, 4541
BstACI GRCGYC 3 cut(s) 17, 141, 4427
BstAUI TGTACA 2 cut(s) 3811, 4411
BstBI TTCGAA 3 cut(s) 781, 1894, 4290
BstC8I GCNNGC 9 cut(s) 125, 574, 996, 1176, 1654, 1686, 2218, 3799, 4116
BstDSI CCRYGG 2 cut(s) 3599, 4527
BstEII GGTNACC 2 cut(s) 835, 3627
BstFNI CGCG 1 cut(s) 4114
BstH2I RGCGCY 3 cut(s) 3130, 3194, 4490
BstHHI GCGC 5 cut(s) 397, 994, 3129, 3193, 4489
BstMAI GTCTC 8 cut(s) 572, 884, 2341, 2475, 2625, 2960, 4160, 4459
BstMCI CGRYCG 1 cut(s) 2562
BstMWI GCNNNNNNNGC 9 cut(s) 25, 318, 1548, 1649, 1694, 3140, 4339, 4433, 4495
BstNI CCWGG 8 cut(s) 101, 804, 2265, 2625, 2946, 2990, 3542, 4305
BstNSI RCATGY 4 cut(s) 590, 679, 2066, 2445
BstPI GGTNACC 2 cut(s) 835, 3627
BstSCI CCNGG 9 cut(s) 99, 802, 1587, 2263, 2623, 2944, 2988, 3540, 4303
BstSFI CTRYAG 3 cut(s) 2614, 3028, 4053
BstSLI GKGCMC 1 cut(s) 942
BstUI CGCG 1 cut(s) 4114
BstX2I RGATCY 8 cut(s) 91, 2330, 2630, 3897, 4217, 4355, 4698, 5221
BstXI CCANNNNNNTGG 2 cut(s) 832, 4534
BstYI RGATCY 8 cut(s) 91, 2330, 2630, 3897, 4217, 4355, 4698, 5221
BstZI CGGCCG 1 cut(s) 2559
Bsu36I CCTNAGG 1 cut(s) 2832
BsuI GTATCC 1 cut(s) 1660
BsuRI GGCC 4 cut(s) 104, 2275, 2561, 3691
BtgI CCRYGG 2 cut(s) 3599, 4527
BtgZI GCGATG 1 cut(s) 1217
BtsI GCAGTG 2 cut(s) 243, 942
Cac8I GCNNGC 9 cut(s) 125, 574, 996, 1176, 1654, 1686, 2218, 3799, 4116
CaiI CAGNNNCTG 1 cut(s) 3881
CciI TCATGA 2 cut(s) 2965, 3019
CfoI GCGC 5 cut(s) 397, 994, 3129, 3193, 4489
Cfr13I GGNCC 2 cut(s) 535, 3689
CseI GACGC 4 cut(s) 25, 579, 617, 4416
CspCI CAANNNNNGTGG 2 cut(s) 931, 966
EaeI YGGCCR 1 cut(s) 2559
EagI CGGCCG 1 cut(s) 2559
Eam1104I CTCTTC 6 cut(s) 288, 778, 802, 3087, 3404, 4541
EarI CTCTTC 6 cut(s) 288, 778, 802, 3087, 3404, 4541
EciI GGCGGA 1 cut(s) 878
Ecl136II GAGCTC 1 cut(s) 3745
EclXI CGGCCG 1 cut(s) 2559
Eco24I GRGCYC 1 cut(s) 3747
Eco31I GGTCTC 1 cut(s) 2625
Eco32I GATATC 1 cut(s) 5362
Eco47I GGWCC 1 cut(s) 535
Eco47III AGCGCT 2 cut(s) 3192, 4488
Eco52I CGGCCG 1 cut(s) 2559
Eco53kI GAGCTC 1 cut(s) 3745
Eco57I CTGAAG 9 cut(s) 446, 660, 1694, 1727, 2618, 2819, 2939, 4242, 4298
Eco81I CCTNAGG 1 cut(s) 2832
Eco91I GGTNACC 2 cut(s) 835, 3627
EcoICRI GAGCTC 1 cut(s) 3745
EcoO109I RGGNCCY 1 cut(s) 535
EcoO65I GGTNACC 2 cut(s) 835, 3627
EcoRI GAATTC 4 cut(s) 707, 1747, 3023, 4159
EcoRII CCWGG 8 cut(s) 99, 802, 2263, 2623, 2944, 2988, 3540, 4303
EcoRV GATATC 1 cut(s) 5362
EcoT22I ATGCAT 4 cut(s) 817, 1346, 3136, 3530
EcoT38I GRGCYC 1 cut(s) 3747
Esp3I CGTCTC 1 cut(s) 2341
FalI AAGNNNNNCTT 6 cut(s) 781, 813, 912, 944, 4327, 4359
FaqI GGGAC 4 cut(s) 2066, 2555, 3214, 3771
FauI CCCGC 1 cut(s) 1927
FauNDI CATATG 1 cut(s) 4972
FbaI TGATCA 8 cut(s) 240, 747, 1690, 2692, 3016, 4804, 4830, 5076
FblI GTMKAC 1 cut(s) 5184
FriOI GRGCYC 1 cut(s) 3747
FspBI CTAG 6 cut(s) 1659, 1824, 1862, 2496, 3477, 4850
GlaI GCGC 5 cut(s) 396, 993, 3128, 3192, 4488
GsuI CTGGAG 4 cut(s) 2646, 2972, 4009, 4746
HaeII RGCGCY 3 cut(s) 3130, 3194, 4490
HaeIII GGCC 4 cut(s) 104, 2275, 2561, 3691
HapII CCGG 5 cut(s) 120, 431, 1588, 2030, 2558
HgaI GACGC 4 cut(s) 25, 579, 617, 4416
HhaI GCGC 5 cut(s) 397, 994, 3129, 3193, 4489
Hin1I GRCGYC 3 cut(s) 17, 141, 4427
Hin6I GCGC 5 cut(s) 395, 992, 3127, 3191, 4487
HinP1I GCGC 5 cut(s) 395, 992, 3127, 3191, 4487
HincII GTYRAC 4 cut(s) 1096, 2174, 2344, 3978
HindII GTYRAC 4 cut(s) 1096, 2174, 2344, 3978
HindIII AAGCTT 4 cut(s) 1219, 1229, 1766, 3828
HpaI GTTAAC 1 cut(s) 1096
HpaII CCGG 5 cut(s) 120, 431, 1588, 2030, 2558
Hpy166II GTNNAC 9 cut(s) 145, 305, 1096, 1390, 1786, 2174, 2344, 3978, 5185
Hpy8I GTNNAC 9 cut(s) 145, 305, 1096, 1390, 1786, 2174, 2344, 3978, 5185
Hpy99I CGWCG 3 cut(s) 13, 611, 2771
HpyCH4IV ACGT 4 cut(s) 141, 775, 2521, 3731
HpyF10VI GCNNNNNNNGC 9 cut(s) 25, 318, 1548, 1649, 1694, 3140, 4339, 4433, 4495
HpySE526I ACGT 4 cut(s) 141, 775, 2521, 3731
Hsp92I GRCGYC 3 cut(s) 17, 141, 4427
HspAI GCGC 5 cut(s) 395, 992, 3127, 3191, 4487
KpnI GGTACC 1 cut(s) 4327
Ksp22I TGATCA 8 cut(s) 240, 747, 1690, 2692, 3016, 4804, 4830, 5076
KspAI GTTAAC 1 cut(s) 1096
LguI GCTCTTC 3 cut(s) 288, 778, 3404
MaeI CTAG 6 cut(s) 1659, 1824, 1862, 2496, 3477, 4850
MaeII ACGT 4 cut(s) 141, 775, 2521, 3731
MbiI CCGCTC 1 cut(s) 1762
MfeI CAATTG 4 cut(s) 561, 765, 1420, 2412
MflI RGATCY 8 cut(s) 91, 2330, 2630, 3897, 4217, 4355, 4698, 5221
MhlI GDGCHC 4 cut(s) 30, 942, 3747, 4802
MlyI GAGTC 5 cut(s) 1082, 3196, 3453, 3604, 4047
Mph1103I ATGCAT 4 cut(s) 817, 1346, 3136, 3530
MroXI GAANNNNTTC 6 cut(s) 162, 777, 1139, 2147, 2462, 4560
MslI CAYNNNNRTG 6 cut(s) 312, 830, 1448, 2846, 3807, 4532
MspA1I CMGCKG 2 cut(s) 638, 4598
MspI CCGG 5 cut(s) 120, 431, 1588, 2030, 2558
MspR9I CCNGG 9 cut(s) 101, 804, 1589, 2265, 2625, 2946, 2990, 3542, 4305
MunI CAATTG 4 cut(s) 561, 765, 1420, 2412
Mva1269I GAATGC 7 cut(s) 175, 668, 2149, 2509, 2982, 3297, 3530
MvaI CCWGG 8 cut(s) 101, 804, 2265, 2625, 2946, 2990, 3542, 4305
MvnI CGCG 1 cut(s) 4114
MwoI GCNNNNNNNGC 9 cut(s) 25, 318, 1548, 1649, 1694, 3140, 4339, 4433, 4495
NciI CCSGG 1 cut(s) 1589
NcoI CCATGG 2 cut(s) 3599, 4527
NdeI CATATG 1 cut(s) 4972
NlaIV GGNNCC 7 cut(s) 537, 1011, 2332, 2908, 3873, 4325, 5223
NmeAIII GCCGAG 1 cut(s) 4488
NmuCI GTSAC 7 cut(s) 835, 1439, 1603, 2444, 2802, 3627, 4428
NsiI ATGCAT 4 cut(s) 817, 1346, 3136, 3530
NspI RCATGY 4 cut(s) 590, 679, 2066, 2445
NspV TTCGAA 3 cut(s) 781, 1894, 4290
PagI TCATGA 2 cut(s) 2965, 3019
PciI ACATGT 1 cut(s) 675
PciSI GCTCTTC 3 cut(s) 288, 778, 3404
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PctI GAATGC 7 cut(s) 175, 668, 2149, 2509, 2982, 3297, 3530
PdmI GAANNNNTTC 6 cut(s) 162, 777, 1139, 2147, 2462, 4560
PflMI CCANNNNNTGG 1 cut(s) 2105
PfoI TCCNGGA 2 cut(s) 2623, 3540
PleI GAGTC 5 cut(s) 1081, 3196, 3452, 3604, 4046
PpsI GAGTC 5 cut(s) 1081, 3196, 3452, 3604, 4046
PpuMI RGGWCCY 1 cut(s) 535
PscI ACATGT 1 cut(s) 675
PsiI TTATAA 2 cut(s) 2184, 2786
Psp124BI GAGCTC 1 cut(s) 3747
Psp5II RGGWCCY 1 cut(s) 535
Psp6I CCWGG 8 cut(s) 99, 802, 2263, 2623, 2944, 2988, 3540, 4303
PspEI GGTNACC 2 cut(s) 835, 3627
PspGI CCWGG 8 cut(s) 99, 802, 2263, 2623, 2944, 2988, 3540, 4303
PspN4I GGNNCC 7 cut(s) 537, 1011, 2332, 2908, 3873, 4325, 5223
PspPI GGNCC 2 cut(s) 535, 3689
PspPPI RGGWCCY 1 cut(s) 535
PstNI CAGNNNCTG 1 cut(s) 3881
PsuI RGATCY 8 cut(s) 91, 2330, 2630, 3897, 4217, 4355, 4698, 5221
PvuII CAGCTG 2 cut(s) 638, 4598
RseI CAYNNNNRTG 6 cut(s) 312, 830, 1448, 2846, 3807, 4532
SacI GAGCTC 1 cut(s) 3747
SapI GCTCTTC 3 cut(s) 288, 778, 3404
Sau96I GGNCC 2 cut(s) 535, 3689
ScaI AGTACT 1 cut(s) 188
SchI GAGTC 5 cut(s) 1082, 3196, 3453, 3604, 4047
ScrFI CCNGG 9 cut(s) 101, 804, 1589, 2265, 2625, 2946, 2990, 3542, 4305
SduI GDGCHC 4 cut(s) 30, 942, 3747, 4802
SfcI CTRYAG 3 cut(s) 2614, 3028, 4053
SfuI TTCGAA 3 cut(s) 781, 1894, 4290
SinI GGWCC 1 cut(s) 535
SmiMI CAYNNNNRTG 6 cut(s) 312, 830, 1448, 2846, 3807, 4532
SmlI CTYRAG 8 cut(s) 211, 929, 2045, 2738, 3853, 4571, 4928, 5013
SmoI CTYRAG 8 cut(s) 211, 929, 2045, 2738, 3853, 4571, 4928, 5013
SpeI ACTAGT 1 cut(s) 1861
SsiI CCGC 5 cut(s) 5, 508, 863, 1762, 1934
SspMI CTAG 6 cut(s) 1659, 1824, 1862, 2496, 3477, 4850
SstI GAGCTC 1 cut(s) 3747
StyD4I CCNGG 9 cut(s) 99, 802, 1587, 2263, 2623, 2944, 2988, 3540, 4303
TaiI ACGT 4 cut(s) 144, 778, 2524, 3734
TatI WGTACW 6 cut(s) 186, 1291, 1389, 3393, 3811, 4411
TauI GCSGC 2 cut(s) 8, 511
TseFI GTSAC 7 cut(s) 835, 1439, 1603, 2444, 2802, 3627, 4428
Tsp45I GTSAC 7 cut(s) 835, 1439, 1603, 2444, 2802, 3627, 4428
TspGWI ACGGA 5 cut(s) 58, 101, 2323, 2784, 5063
Van91I CCANNNNNTGG 1 cut(s) 2105
VpaK11BI GGWCC 1 cut(s) 535
XceI RCATGY 4 cut(s) 590, 679, 2066, 2445
XcmI CCANNNNNNNNNTGG 1 cut(s) 2996
XmaJI CCTAGG 1 cut(s) 3476
XmiI GTMKAC 1 cut(s) 5184
XmnI GAANNNNTTC 6 cut(s) 162, 777, 1139, 2147, 2462, 4560
XspI CTAG 6 cut(s) 1659, 1824, 1862, 2496, 3477, 4850
ZraI GACGTC 1 cut(s) 142
ZrmI AGTACT 1 cut(s) 188
Zsp2I ATGCAT 4 cut(s) 817, 1346, 3136, 3530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.