Rh7DG103200

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000526
Physical Location & Seq
Forward (+)
8723003 .. 8730656
7654 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG103200.1

Sequence Viewer

Length: 4974 bp
ATGGCGACGACGATGACGCCAAGGGAGCACATAGAAGATATACGGAAGACCAAGTTCTCCATCGGAGAAAAGCTCAACCCTTTAACGGAGGATCTTCACCAGGCCGTCAAGAACCTCTCCGGCGAGCTTTACTCTAAAGACGTCCACTTTTTCATGGAACTCGTTCAGAATGCAGAAGACAATGAGTACTTGGAGGGGGTCGATCCATCCCTTGAGTTTGTGATTACATACCTCGATATAACGGCTACAGGGGCTCCAGCCACATTGCTGGTTTTCAATAATGAGAAGGGGTTTTCTCCCAAAAACATAGACTCCATTTGCAGTGTTGGACGGTCCACCAAGAAAGGCAACAGGAAGCGTGGTTATATTGGGGAGAAAGGAATTGGCTTCAAGAGTGTGTTTCTGATCACTGCTCAGCCTTACATATTTAGTAATGGTTATCAGATAAGGTTCAGCGAAGAGCCTTGTTCACATTGCAATGTTGGCTACATAGTACCTGAATGGGTTGAGCAGAATCCCACACTATCTGATATAAAAAAGATATATGGTTCTGCTACTGCCCTTCCTACCACAACACTGATCTTACCTCTGAAGCCCAACAAGGTTGAACCTGTGAAGAAACAACTCTCAATCATTCATCCAGAAGTTCTCTTGTTCCTATCAAAGATTAAGCGGCTCTCTGTTAGGGAAGACAAAGAGGATCCAAGGCAGAACACTGTGAGTGCAATTGAGATAGCAAGCCAGACTGAGTTTGTGTCAAGGAAGAACATCGATGCTCAATCCTACACTCTGCATCTTTCAGCTGAAGAAAATGATGGAAATGGAGAATGCAGTTACTACATGTGGAAACAAAAGTTTCCTGTCAGACAGGAATTCAGAGTTGAGAGGAGAATGGAGGTGGATGAGTGGGTGATCACTCTTGCATTTCCAAATGGAGAACGTCTTCGAAGAGCAAGGAACTCATCGCCTGGTGTTTATGCATTTCTTCCTACAGAGATGGTCACCAACTTTCCCTTCATAATACAGGCTGATTTTCTTTTAGCATCTTCAAGAGAGACAATCCTACTTGACAACAAGTGGAATCAAGGGATTCTTGAGTGTGTGCCCACCGCATTTTCACATGCTTTTAACTCTCTTGTGAAAACTGTGGAAGGTGCGCCAACATCAAGTTTGCCTCCTATGTTCAGATTCTTGCCTGTTCAGGTTTCTTCCTATAAAGAATTAAATGCTGTAAGGGAGTCAATAAAAGCAAAGATGGTTGACGAAGACATTATACCAGTTGAGCCTGAAATGGAGCAGAGGTTCTTCCACAAGCCTCGTGAAGTGGGAAGATTAATGCCTGCCTTTTGGACTATTTTGAGGAAGGCGAGGGTTCAGGGTGTAAGCTTGATTAAGCTTTCATCACATGGAAGATTTGTTCTGTCTTATTCCTTTGACAGCAAGGAATATGATCCTATACTAAGTTTCCTTGGAGTTGAACTAGTGAATGATGCCTGGTATGTTAAATGCATACAGGGTACTTCTGATGTTGTTGATCGAGTGTCAGAAGATGTGTACTTAGAGCTTCTACTGTTTATCGCTGACAATTGGGCTTCTAAATTTGGTCACACCAACATGAAGAACATACCATTGATAAAATATGCAGACTGTAGAAATGAATCTTTATGCAGCATAAGCGAAATCCAGAAAGGTGAATGTAGAGTTTTCTTATCGAGCAAAAGTTCTCATGTTTCATGGCTGATTGATTGCAACCGGGAATTTAATTCTGTGACTCGTCTTCTCTTTATGCCAAAAGCCACTCAGGAAGCTATCTGGGCATGCTCTGGGAAGGATACATTGAAGAAATGGCTAGCTGATCAAGCTAAGGTTGGTTCTGTTGATGTCAATAAATATGCAGTTGATCTACTTCAGAATTCTCTTCATGAGCGGAAGCTTGTTATTGCATATGTTCATTTCTTATATCACTCGGCTGATAATAATTTTATCTCAAGTCGGGAAGTTGACCGTTTATGTGGAATTATGCCACTAGTGGATGGCTATGGGAACATCATCAGAAGTAGAAAAGGCGTTGTTGTTGTTCCTGCCAATGGAAGCAAATGGGCGGGATTGACTGATTCTAATTTGTGGAGAAAAGAAGGCCATGTTGAATTAGGGGAGCATTATATGTATTCTGGCTACTTTTCTGGTAAGTATACACCGGAGAAGAAGCTTCTTGAGTTCCTTATAAAACATGTTGCTGCTTTGGATGTCCCTCATATTTCTGCCCCAAGTGATGGTATCACGTCTGTATCTGCACCACTAACTAAGCAGAATGCCTTCTTGCTTTTGGATTGGATTCGTCAACTGAATTATAAAAGGGTTGGCATCCCTCAGAAGTTCTTGGCTTGCATAAACGAAGGTAGTTGGCTGAAAGTTACTTTGAATGGCTCTCCTGGTTATAGGCCGCCATCCCAGTCATTTCTGCTTAACTCATCATGGGGAAATACTTTGCAGAATGGATCCGTCTCTGTTGACATTCCATTGATTGATACGAGCTTTTATGGTGAGAGCATTAGTGGATACAAGGAGGAGCTAAAGACAATTGGGGTCATGTTTGAATATAGCGAAGCATGTGACTTCATTGGGAAGCATTTCATGTCTCTGGCAGCTTCATCCTCTTTAACTAGAGGAAATGTGCATTCCATTCTGCGTTTCATCAGGTTTTTAAGGGACAAACTTCTTTCTCCGGCCGATTTTATCTCTGCTATTAAGAAAGGGAACTGGCTGAAGACTTCCGTTGGCTACAGGTCTCCTGTAGGATCTGTTTTGTCAGATAAGGAGTGGAATGTTGCCTCCAAAATTAGCAACATTCCCTTCATTGATCAGGACTTTTACGGAGATGAAATCTGTAATTTCAGAACTGAACTTGGGCTGCTTGGTGTTATAATCTCCTTCAACAGAAGTTACCAACTTATAATTGACAACCTGAAGCCACCATCTTGCATAACTTCTTGGACACCTGAGGTTGTTACCTTAATGCTCAACTGTATGGCTCTATCCAAATCATCTGACAAACTTGTCAATGCACTGAAAGGAGTCAAGTGCCTGAAGACAATTATTGGTTACAAGTGTCCAGGGGAATGTCTCTTGTTTCATCACGAATGGGGTTGCATTCTTCAGGTCGTCAATGGTCTTCCCGTGATTGATCATGAATTCTATGGGAGCAACATTTTCCAGTACATAAATGAACTGAAAAAAATTGGTGTGGTGGTTGATTTTGAAGGGGCTGCAAAAGTACTTGCTCGATATTTCAGGCACTATGCAAAATCAGCTTCCATCACCAAGGAAAATGTTGCATCATTGCTTCTGTGTTACAGAGAGTTGAATGGGACTCCATATAAATTTCCTGCTGATATTAGGAGCTGCTTCGCTAAGGGCATACATGAGTACAGAAAAGAGTTGAAGAGCTTGGGGGTTGTCGTCGAATACAAGGAAGGAGTTAAGTTTGTGGCTCGTGGTCTTTATCTCCCTAGGGATGCCAGCAGTATTTTTCCTGTAAATGCACTTGCGTTGCTGGAATGCATACGTGTTTTCCAGAAGAAAGGTTACGCCTTTCTTGATAATTCTGATTTCATGAAGAAAGTTTCTCAACCATGGATAAAGACTCATGCTGGTTATTGGTCTCCCAAAGAGTCTTTGTTGTTTGATTCCAAGTTTGGCTTATATTTGAAGCGGACTGATGGGCCTTTCATTGATGAAGAATTCTATGGTACTAAGATTGCATCTTACAAAAAAGAGCTGTCTGCATTGGGGGTTATTGTTGAAGCTGAAAAGGGATGTCCCTTGATTGCCGGCCACCTTGATATTCATGATGAGACTGAAGCTTTTGTTCGGTTGTATAATTACTTGAGTGAGTTCCAGTGGGAGCCAGACACTGACGCTGCTCGAAGGATCTGGATTCCAAAAGGGGAGTGGGTTCGTGCTGATGAATGTGTTATTTATGACAAGGATGGGCTATTTGGTTTGCAGTTGACTGTTTTGGAGAAACATTATGAGCATAAACTTCTGCTTTTCTTCTCCAGTGCGTTTGGAGTTAAATCTCATCCTTCAGTTGATGATTACTTGAAGGTTTGGAAGGTTTGGGAAAGTTCTGGAAGTGCATTGTCGCATGCTGATTGTTGCAAGTTTTGGAGTTATATCTCGAAGCACTGGAATTCAAAGACAGAAAAGACTGTTGCTGATGCCTTGGTGAAAGTACCTGTCAGTTCTGGATCTGAAGGAATCTTGCTGTGCTACAAGTCTGATGTTTTCATTGCTGATGACCTTCATCTGAAGTATCTATTCGAACAGTCTTCTCCCGAGTCTATTTTTGTTTGGTACCCTCAACCAAGTTTGCCTTCCATTCCAAGATCCAAGTTGCTTGACATTTATCGAAGAATTGGTGTTCGCACTATATCTGAATCTGTACAAAAGGAAGAGGCGTCACTTGCAGATGATGTTGAAGCAGTCTCTTTGCCGAGGGAAAAGCTGATTAAGAAAGCGCTGTTCAGGCTGATTCTTGGTTTTCTTGCTGCTCCTCCCATGGAAATGGAAGCTGAGAAGAGGCTTGAAGCTGTTCAATTCCTTGTGAATGTTACTGTGGTAGTTACAGCTGAACCAATCTCTGTAAGCTATAGTCTACCTTTATCTTCTTGGAAAGTTTTGAATGTGAGAGGAAGCCAAAAGATACGTTTTGATAGAGACAATTCAAAGATCTTCACTCAGAAAATGGACAAGTCTGGAGGGCAGAAGAGTATGTTTGAGTTTGCTACTTCTTTCTCTCAAGCAATATCTGAGAGTGTGGTATGGGATAGTACGGATCATATAGATTCACTCTCTGAGCTGATCAAGGTGGCTGCTTTGCTAAACTTCAAAGAGGAAGCAGTTGATTCCTTGATGAAGTCAAAGAATTTGCAAATCTTCATGGAGGATGAGGAGTTTCTCAGGACTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1657

Amino Acids

187.62

Weight (kDa)

7.33

Isoelectric Point (pI)

36.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 30 - 146 9.9e-11 Sacsin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28020 AT3G48770 AT3G48770
fragaria_vesca FvH4_5g09020 FvH4_5g09020
malus_domestica MD06G1092400.v1.1 MD06G1134600.v1.1 MD06G1134700.v1.1 MD06G1134800.v1.1 MD06G1135200.v1.1 MD06G1135400.v1.1 MD06G1135500.v1.1 MD08G1137600.v1.1 MD15G1395100.v1.1
prunus_persica Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145600_v2.0.a1 Prupe.5G145800_v2.0.a1 Prupe.5G145900_v2.0.a1
pyrus_communis pycom06g12550 pycom06g12570 pycom06g12580 pycom06g12590 pycom06g12610 pycom06g12630 pycom06g12640 pycom06g12650 pycom08g06280
rosa_chinensis RchiOBHm_Chr6g0249321 RchiOBHm_Chr6g0275111 RchiOBHm_Chr7g0189191 RchiOBHm_Chr7g0189261
rosa_laevigata RLG00000004622 RLG00000004623 RLG00000008237 RLG00000008238 RLG00000023303 RLG00000035187
rosa_multiflora Rmu_co8294311.1_g000001 Rmu_co8389833.1_g000001 Rmu_sc0001044.1_g000004 Rmu_sc0001329.1_g000011 Rmu_sc0006012.1_g000010 Rmu_sc0007286.1_g000032 Rmu_sc0009360.1_g000016 Rmu_sc0011817.1_g000001 Rmu_sc0015050.1_g000001
rosa_roxburghii Rroxscaffold_1G00064000 Rroxscaffold_3G00265460 Rroxscaffold_3G00265580 Rroxscaffold_3G00265640 Rroxscaffold_3G00265690 Rroxscaffold_3G00265700 Rroxscaffold_7G00193620 Rroxscaffold_7G00204550 Rroxscaffold_7G00217350
rosa_rugosa Rorug01G0465500 Rorug06G0088500 Rorug06G0496700 Rorug06G0496700
rosa_samantha Rh1AG064800 Rh2BG015100 Rh2BG493500 Rh2DG017300 Rh2DG502500 Rh4BG208500 Rh5BG528500 Rh5CG552900 Rh6CG207800 Rh6DG197900 Rh6DG198000 Rh7AG101300 Rh7AG101400 Rh7AG101600 Rh7BG104100 Rh7BG104200 Rh7CG105600 Rh7CG105700 Rh7DG103200 Rh7DG103400
rosa_wichuraiana Rw1G007300 Rw5G034670 Rw7G008750 Rw7G008780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 2225, 2352, 2924, 2954
AasI GACNNNNNNGTC 1 cut(s) 3056
AatII GACGTC 1 cut(s) 144
Acc65I GGTACC 1 cut(s) 4356
AccB1I GGYRCC 1 cut(s) 4356
AccB7I CCANNNNNTGG 1 cut(s) 2273
AccBSI CCGCTC 1 cut(s) 1927
AccI GTMKAC 2 cut(s) 2192, 4657
AciI CCGC 6 cut(s) 673, 1110, 1927, 2102, 2444, 3712
AcoI YGGCCR 2 cut(s) 2727, 3832
AcsI RAATTY 9 cut(s) 872, 1598, 1757, 1912, 3191, 3380, 3740, 4192, 4927
AcyI GRCGYC 3 cut(s) 17, 141, 4460
AfeI AGCGCT 1 cut(s) 4521
AfiI CCNNNNNNNGG 3 cut(s) 85, 2087, 2273
AflIII ACRYGT 3 cut(s) 840, 2230, 3565
AhlI ACTAGT 2 cut(s) 1480, 2026
AjiI CACGTC 1 cut(s) 2283
AjnI CCWGG 5 cut(s) 99, 967, 1493, 2431, 3112
AjuI GAANNNNNNNTTGG 4 cut(s) 998, 1030, 4360, 4392
AloI GAACNNNNNNTCC 4 cut(s) 1286, 1318, 1401, 1433
Alw21I GWGCWC 1 cut(s) 30
Alw26I GTCTC 9 cut(s) 1049, 2509, 2643, 2793, 3128, 3666, 3848, 4492, 4713
AlwNI CAGNNNCTG 1 cut(s) 3914
Ama87I CYCGRG 1 cut(s) 4337
Aor51HI AGCGCT 1 cut(s) 4521
AoxI GGCC 6 cut(s) 102, 2137, 2441, 2727, 3722, 3832
ApeKI GCWGC 9 cut(s) 1668, 2237, 2645, 2911, 3266, 3402, 3920, 4550, 4874
ApoI RAATTY 9 cut(s) 872, 1598, 1757, 1912, 3191, 3380, 3740, 4192, 4927
AseI ATTAAT 1 cut(s) 1334
Asp700I GAANNNNTTC 5 cut(s) 162, 942, 2315, 2630, 4593
Asp718I GGTACC 1 cut(s) 4356
AspA2I CCTAGG 1 cut(s) 3509
AspLEI GCGC 2 cut(s) 1159, 4522
AspS9I GGNCC 2 cut(s) 333, 3722
AsuC2I CCSGG 1 cut(s) 1754
AsuHPI GGTGA 7 cut(s) 89, 922, 994, 1703, 2555, 3310, 4240
AsuII TTCGAA 2 cut(s) 946, 4323
AsuNHI GCTAGC 1 cut(s) 1849
AvaI CYCGRG 1 cut(s) 4337
AvaII GGWCC 1 cut(s) 333
AvrII CCTAGG 1 cut(s) 3509
AxyI CCTNAGG 1 cut(s) 3000
BaeGI GKGCMC 1 cut(s) 1107
BaeI ACNNNNGTAYC 2 cut(s) 2271, 2304
BamHI GGATCC 2 cut(s) 700, 2498
BanI GGYRCC 1 cut(s) 4356
BanII GRGCYC 1 cut(s) 256
BarI GAAGNNNNNNTAC 4 cut(s) 1257, 1289, 3569, 3601
BauI CACGAG 2 cut(s) 1317, 3492
Bbv12I GWGCWC 1 cut(s) 30
BbvI GCAGC 9 cut(s) 1680, 2224, 2657, 2898, 3253, 3389, 3907, 4537, 4861
BceAI ACGGC 2 cut(s) 89, 258
BciT130I CCWGG 5 cut(s) 101, 969, 1495, 2433, 3114
BciVI GTATCC 2 cut(s) 1825, 2552
BclI TGATCA 6 cut(s) 405, 912, 1855, 2860, 3184, 4863
BcnI CCSGG 1 cut(s) 1754
BcoDI GTCTC 9 cut(s) 1049, 2509, 2643, 2793, 3128, 3666, 3848, 4492, 4713
BcuI ACTAGT 2 cut(s) 1480, 2026
BfaI CTAG 6 cut(s) 1481, 1850, 2027, 2664, 3510, 4972
BfmI CTRYAG 6 cut(s) 246, 990, 1648, 2782, 2793, 4651
BfoI RGCGCY 1 cut(s) 4523
BfuI GTATCC 2 cut(s) 1825, 2552
BglII AGATCT 1 cut(s) 4731
BlnI CCTAGG 1 cut(s) 3509
BlpI GCTNAGC 1 cut(s) 414
BmcAI AGTACT 2 cut(s) 188, 3276
Bme1390I CCNGG 6 cut(s) 101, 969, 1495, 1754, 2433, 3114
Bme18I GGWCC 1 cut(s) 333
BmeT110I CYCGRG 1 cut(s) 4337
BmgBI CACGTC 1 cut(s) 2283
BmgT120I GGNCC 2 cut(s) 333, 3722
BmiI GGNNCC 5 cut(s) 255, 702, 2500, 3906, 4358
BmrFI CCNGG 6 cut(s) 101, 969, 1495, 1754, 2433, 3114
BmrI ACTGGG 1 cut(s) 2446
BmsI GCATC 9 cut(s) 763, 802, 1052, 1480, 2373, 3344, 3505, 3770, 4210
BmtI GCTAGC 1 cut(s) 1853
BmuI ACTGGG 1 cut(s) 2446
BplI GAGNNNNNCTC 4 cut(s) 57, 89, 116, 148
BpmI CTGGAG 3 cut(s) 240, 4042, 4779
Bpu10I CCTNAGC 2 cut(s) 1863, 3411
Bpu1102I GCTNAGC 1 cut(s) 414
Bpu14I TTCGAA 2 cut(s) 946, 4323
BpuEI CTTGAG 6 cut(s) 233, 1115, 1972, 2234, 3907, 4785
BpuMI CCSGG 1 cut(s) 1754
Bsa29I ATCGAT 1 cut(s) 771
BsaAI YACGTR 1 cut(s) 3566
BsaHI GRCGYC 3 cut(s) 17, 141, 4460
BsaI GGTCTC 2 cut(s) 2793, 3666
BsaWI WCCGGW 1 cut(s) 2197
BsaXI ACNNNNNCTCC 6 cut(s) 238, 268, 296, 326, 1286, 1316
Bsc4I CCNNNNNNNGG 3 cut(s) 85, 2087, 2273
Bse118I RCCGGY 1 cut(s) 3830
Bse1I ACTGG 7 cut(s) 1277, 2452, 2765, 3214, 3898, 4059, 4193
Bse21I CCTNAGG 1 cut(s) 3000
Bse3DI GCAATG 5 cut(s) 263, 472, 484, 3338, 4290
BseBI CCWGG 5 cut(s) 101, 969, 1495, 2433, 3114
BseCI ATCGAT 1 cut(s) 771
BseLI CCNNNNNNNGG 3 cut(s) 85, 2087, 2273
BseMI GCAATG 5 cut(s) 263, 472, 484, 3338, 4290
BseMII CTCAG 9 cut(s) 428, 738, 1814, 2384, 2991, 4566, 4754, 4803, 4848
BseNI ACTGG 7 cut(s) 1277, 2452, 2765, 3214, 3898, 4059, 4193
BseRI GAGGAG 4 cut(s) 901, 2582, 4545, 4967
BseSI GKGCMC 1 cut(s) 1107
BseX3I CGGCCG 1 cut(s) 2727
BseXI GCAGC 9 cut(s) 1680, 2224, 2657, 2898, 3253, 3389, 3907, 4537, 4861
BsgI GTGCAG 1 cut(s) 2277
Bsh1285I CGRYCG 1 cut(s) 2730
BshFI GGCC 6 cut(s) 104, 2139, 2443, 2729, 3724, 3834
BshNI GGYRCC 1 cut(s) 4356
BshVI ATCGAT 1 cut(s) 771
BsiEI CGRYCG 1 cut(s) 2730
BsiHKAI GWGCWC 1 cut(s) 30
BsiHKCI CYCGRG 1 cut(s) 4337
BsiSI CCGG 5 cut(s) 120, 1753, 2198, 2726, 3831
BslFI GGGAC 4 cut(s) 2234, 2723, 3382, 3804
BslI CCNNNNNNNGG 3 cut(s) 85, 2087, 2273
BsmAI GTCTC 9 cut(s) 1049, 2509, 2643, 2793, 3128, 3666, 3848, 4492, 4713
BsmBI CGTCTC 1 cut(s) 2509
BsmFI GGGAC 4 cut(s) 2234, 2723, 3382, 3804
BsmI GAATGC 6 cut(s) 175, 833, 2317, 2677, 3150, 3563
BsnI GGCC 6 cut(s) 104, 2139, 2443, 2729, 3724, 3834
Bso31I GGTCTC 2 cut(s) 2793, 3666
BsoBI CYCGRG 1 cut(s) 4337
Bsp119I TTCGAA 2 cut(s) 946, 4323
Bsp1286I GDGCHC 3 cut(s) 30, 256, 1107
Bsp1407I TGTACA 1 cut(s) 4444
Bsp1720I GCTNAGC 1 cut(s) 414
Bsp19I CCATGG 2 cut(s) 3632, 4560
BspACI CCGC 6 cut(s) 673, 1110, 1927, 2102, 2444, 3712
BspANI GGCC 6 cut(s) 104, 2139, 2443, 2729, 3724, 3834
BspDI ATCGAT 1 cut(s) 771
BspHI TCATGA 4 cut(s) 1921, 3187, 3612, 3847
BspLI GGNNCC 5 cut(s) 255, 702, 2500, 3906, 4358
BspOI GCTAGC 1 cut(s) 1853
BspQI GCTCTTC 3 cut(s) 453, 943, 3437
BspT104I TTCGAA 2 cut(s) 946, 4323
BspT107I GGYRCC 1 cut(s) 4356
BspTNI GGTCTC 2 cut(s) 2793, 3666
BsrBI CCGCTC 1 cut(s) 1927
BsrDI GCAATG 5 cut(s) 263, 472, 484, 3338, 4290
BsrFI RCCGGY 1 cut(s) 3830
BsrGI TGTACA 1 cut(s) 4444
BsrI ACTGG 7 cut(s) 1277, 2452, 2765, 3214, 3898, 4059, 4193
BssAI RCCGGY 1 cut(s) 3830
BssNAI GTATAC 1 cut(s) 2193
BssNI GRCGYC 3 cut(s) 17, 141, 4460
BssSI CACGAG 2 cut(s) 1317, 3492
BssT1I CCWWGG 8 cut(s) 20, 704, 1468, 3321, 3509, 3632, 4224, 4560
Bst1107I GTATAC 1 cut(s) 2193
Bst2BI CACGAG 2 cut(s) 1317, 3492
Bst2UI CCWGG 5 cut(s) 101, 969, 1495, 2433, 3114
Bst6I CTCTTC 7 cut(s) 453, 943, 1923, 3437, 4449, 4574, 4763
BstACI GRCGYC 3 cut(s) 17, 141, 4460
BstAUI TGTACA 1 cut(s) 4444
BstBAI YACGTR 1 cut(s) 3566
BstBI TTCGAA 2 cut(s) 946, 4323
BstC8I GCNNGC 9 cut(s) 125, 739, 1341, 1819, 1851, 2386, 3520, 3832, 4149
BstDSI CCRYGG 2 cut(s) 3632, 4560
BstEII GGTNACC 1 cut(s) 1000
BstH2I RGCGCY 1 cut(s) 4523
BstHHI GCGC 2 cut(s) 1159, 4522
BstMAI GTCTC 9 cut(s) 1049, 2509, 2643, 2793, 3128, 3666, 3848, 4492, 4713
BstMCI CGRYCG 1 cut(s) 2730
BstMWI GCNNNNNNNGC 9 cut(s) 25, 251, 483, 1674, 1814, 1859, 3308, 4466, 4528
BstNI CCWGG 5 cut(s) 101, 969, 1495, 2433, 3114
BstNSI RCATGY 6 cut(s) 844, 1124, 1821, 2234, 2613, 4151
BstPI GGTNACC 1 cut(s) 1000
BstSCI CCNGG 6 cut(s) 99, 967, 1493, 1752, 2431, 3112
BstSFI CTRYAG 6 cut(s) 246, 990, 1648, 2782, 2793, 4651
BstSLI GKGCMC 1 cut(s) 1107
BstV1I GCAGC 9 cut(s) 1680, 2224, 2657, 2898, 3253, 3389, 3907, 4537, 4861
BstX2I RGATCY 8 cut(s) 91, 700, 2498, 2798, 3930, 4250, 4388, 4731
BstXI CCANNNNNNTGG 2 cut(s) 268, 4567
BstYI RGATCY 8 cut(s) 91, 700, 2498, 2798, 3930, 4250, 4388, 4731
BstZ17I GTATAC 1 cut(s) 2193
BstZI CGGCCG 1 cut(s) 2727
Bsu15I ATCGAT 1 cut(s) 771
Bsu36I CCTNAGG 1 cut(s) 3000
BsuI GTATCC 2 cut(s) 1825, 2552
BsuRI GGCC 6 cut(s) 104, 2139, 2443, 2729, 3724, 3834
BsuTUI ATCGAT 1 cut(s) 771
BtgI CCRYGG 2 cut(s) 3632, 4560
BtgZI GCGATG 1 cut(s) 948
BtrI CACGTC 1 cut(s) 2283
BtsI GCAGTG 2 cut(s) 328, 408
BtsIMutI CAGTG 9 cut(s) 328, 408, 575, 714, 3065, 3905, 3912, 4066, 4186
Cac8I GCNNGC 9 cut(s) 125, 739, 1341, 1819, 1851, 2386, 3520, 3832, 4149
CaiI CAGNNNCTG 1 cut(s) 3914
CciI TCATGA 4 cut(s) 1921, 3187, 3612, 3847
CfoI GCGC 2 cut(s) 1159, 4522
Cfr10I RCCGGY 1 cut(s) 3830
Cfr13I GGNCC 2 cut(s) 333, 3722
ClaI ATCGAT 1 cut(s) 771
CseI GACGC 3 cut(s) 25, 3926, 4449
DrdI GACNNNNNNGTC 1 cut(s) 3056
DseDI GACNNNNNNGTC 1 cut(s) 3056
EaeI YGGCCR 2 cut(s) 2727, 3832
EagI CGGCCG 1 cut(s) 2727
Eam1104I CTCTTC 7 cut(s) 453, 943, 1923, 3437, 4449, 4574, 4763
EarI CTCTTC 7 cut(s) 453, 943, 1923, 3437, 4449, 4574, 4763
EclXI CGGCCG 1 cut(s) 2727
Eco130I CCWWGG 8 cut(s) 20, 704, 1468, 3321, 3509, 3632, 4224, 4560
Eco24I GRGCYC 1 cut(s) 256
Eco31I GGTCTC 2 cut(s) 2793, 3666
Eco47I GGWCC 1 cut(s) 333
Eco47III AGCGCT 1 cut(s) 4521
Eco52I CGGCCG 1 cut(s) 2727
Eco81I CCTNAGG 1 cut(s) 3000
Eco88I CYCGRG 1 cut(s) 4337
Eco91I GGTNACC 1 cut(s) 1000
EcoO65I GGTNACC 1 cut(s) 1000
EcoRI GAATTC 5 cut(s) 872, 1912, 3191, 3740, 4192
EcoRII CCWGG 5 cut(s) 99, 967, 1493, 2431, 3112
EcoT14I CCWWGG 8 cut(s) 20, 704, 1468, 3321, 3509, 3632, 4224, 4560
EcoT22I ATGCAT 3 cut(s) 982, 1511, 3563
EcoT38I GRGCYC 1 cut(s) 256
ErhI CCWWGG 8 cut(s) 20, 704, 1468, 3321, 3509, 3632, 4224, 4560
Esp3I CGTCTC 1 cut(s) 2509
FalI AAGNNNNNCTT 6 cut(s) 1077, 1109, 3683, 3715, 4360, 4392
FaqI GGGAC 4 cut(s) 2234, 2723, 3382, 3804
FauI CCCGC 1 cut(s) 2095
FauNDI CATATG 1 cut(s) 1945
FbaI TGATCA 6 cut(s) 405, 912, 1855, 2860, 3184, 4863
FblI GTMKAC 2 cut(s) 2192, 4657
FriOI GRGCYC 1 cut(s) 256
FspBI CTAG 6 cut(s) 1481, 1850, 2027, 2664, 3510, 4972
GlaI GCGC 2 cut(s) 1158, 4521
GsuI CTGGAG 3 cut(s) 240, 4042, 4779
HaeII RGCGCY 1 cut(s) 4523
HaeIII GGCC 6 cut(s) 104, 2139, 2443, 2729, 3724, 3834
HapII CCGG 5 cut(s) 120, 1753, 2198, 2726, 3831
HgaI GACGC 3 cut(s) 25, 3926, 4449
HhaI GCGC 2 cut(s) 1159, 4522
Hin1I GRCGYC 3 cut(s) 17, 141, 4460
Hin6I GCGC 2 cut(s) 1157, 4520
HinP1I GCGC 2 cut(s) 1157, 4520
HincII GTYRAC 5 cut(s) 1261, 2002, 2342, 2512, 4011
HindII GTYRAC 5 cut(s) 1261, 2002, 2342, 2512, 4011
HindIII AAGCTT 5 cut(s) 1384, 1394, 1931, 2207, 3861
HpaII CCGG 5 cut(s) 120, 1753, 2198, 2726, 3831
HphI GGTGA 7 cut(s) 89, 922, 994, 1703, 2555, 3310, 4240
Hpy99I CGWCG 3 cut(s) 10, 13, 3464
HpyCH4IV ACGT 5 cut(s) 141, 940, 2282, 3565, 4708
HpyF10VI GCNNNNNNNGC 9 cut(s) 25, 251, 483, 1674, 1814, 1859, 3308, 4466, 4528
HpySE526I ACGT 5 cut(s) 141, 940, 2282, 3565, 4708
Hsp92I GRCGYC 3 cut(s) 17, 141, 4460
HspAI GCGC 2 cut(s) 1157, 4520
KpnI GGTACC 1 cut(s) 4360
KroI GCCGGC 1 cut(s) 3830
KroNI GCCGGC 1 cut(s) 3832
Ksp22I TGATCA 6 cut(s) 405, 912, 1855, 2860, 3184, 4863
LguI GCTCTTC 3 cut(s) 453, 943, 3437
LmnI GCTCC 9 cut(s) 25, 259, 1294, 2155, 2569, 3201, 3399, 3904, 4558
Lsp1109I GCAGC 9 cut(s) 1680, 2224, 2657, 2898, 3253, 3389, 3907, 4537, 4861
LweI GCATC 9 cut(s) 763, 802, 1052, 1480, 2373, 3344, 3505, 3770, 4210
MaeI CTAG 6 cut(s) 1481, 1850, 2027, 2664, 3510, 4972
MaeII ACGT 5 cut(s) 141, 940, 2282, 3565, 4708
MbiI CCGCTC 1 cut(s) 1927
MfeI CAATTG 3 cut(s) 726, 1585, 2580
MflI RGATCY 8 cut(s) 91, 700, 2498, 2798, 3930, 4250, 4388, 4731
MhlI GDGCHC 3 cut(s) 30, 256, 1107
MlyI GAGTC 8 cut(s) 305, 1247, 1765, 3084, 3364, 3637, 3680, 4349
MmeI TCCRAC 1 cut(s) 307
Mph1103I ATGCAT 3 cut(s) 982, 1511, 3563
MroNI GCCGGC 1 cut(s) 3830
MroXI GAANNNNTTC 5 cut(s) 162, 942, 2315, 2630, 4593
MslI CAYNNNNRTG 3 cut(s) 477, 1613, 4565
MspA1I CMGCKG 2 cut(s) 803, 4631
MspI CCGG 5 cut(s) 120, 1753, 2198, 2726, 3831
MspR9I CCNGG 6 cut(s) 101, 969, 1495, 1754, 2433, 3114
MunI CAATTG 3 cut(s) 726, 1585, 2580
Mva1269I GAATGC 6 cut(s) 175, 833, 2317, 2677, 3150, 3563
MvaI CCWGG 5 cut(s) 101, 969, 1495, 2433, 3114
MwoI GCNNNNNNNGC 9 cut(s) 25, 251, 483, 1674, 1814, 1859, 3308, 4466, 4528
NaeI GCCGGC 1 cut(s) 3832
NciI CCSGG 1 cut(s) 1754
NcoI CCATGG 2 cut(s) 3632, 4560
NdeI CATATG 1 cut(s) 1945
NgoMIV GCCGGC 1 cut(s) 3830
NheI GCTAGC 1 cut(s) 1849
NlaIV GGNNCC 5 cut(s) 255, 702, 2500, 3906, 4358
NmeAIII GCCGAG 2 cut(s) 1946, 4521
NmuCI GTSAC 5 cut(s) 1000, 1604, 1768, 2612, 4461
NsiI ATGCAT 3 cut(s) 982, 1511, 3563
NspI RCATGY 6 cut(s) 844, 1124, 1821, 2234, 2613, 4151
NspV TTCGAA 2 cut(s) 946, 4323
PaeI GCATGC 2 cut(s) 1821, 4151
PagI TCATGA 4 cut(s) 1921, 3187, 3612, 3847
PciI ACATGT 2 cut(s) 840, 2230
PciSI GCTCTTC 3 cut(s) 453, 943, 3437
PctI GAATGC 6 cut(s) 175, 833, 2317, 2677, 3150, 3563
PdiI GCCGGC 1 cut(s) 3832
PdmI GAANNNNTTC 5 cut(s) 162, 942, 2315, 2630, 4593
PflMI CCANNNNNTGG 1 cut(s) 2273
PleI GAGTC 8 cut(s) 305, 1246, 1765, 3083, 3364, 3637, 3679, 4348
PpsI GAGTC 8 cut(s) 305, 1246, 1765, 3083, 3364, 3637, 3679, 4348
Ppu21I YACGTR 1 cut(s) 3566
PscI ACATGT 2 cut(s) 840, 2230
PshBI ATTAAT 1 cut(s) 1334
PsiI TTATAA 4 cut(s) 2225, 2352, 2924, 2954
Psp6I CCWGG 5 cut(s) 99, 967, 1493, 2431, 3112
PspEI GGTNACC 1 cut(s) 1000
PspGI CCWGG 5 cut(s) 99, 967, 1493, 2431, 3112
PspN4I GGNNCC 5 cut(s) 255, 702, 2500, 3906, 4358
PspPI GGNCC 2 cut(s) 333, 3722
PstNI CAGNNNCTG 1 cut(s) 3914
PsuI RGATCY 8 cut(s) 91, 700, 2498, 2798, 3930, 4250, 4388, 4731
PvuII CAGCTG 2 cut(s) 803, 4631
RseI CAYNNNNRTG 3 cut(s) 477, 1613, 4565
SapI GCTCTTC 3 cut(s) 453, 943, 3437
Sau96I GGNCC 2 cut(s) 333, 3722
ScaI AGTACT 2 cut(s) 188, 3276
SchI GAGTC 8 cut(s) 305, 1247, 1765, 3084, 3364, 3637, 3680, 4349
ScrFI CCNGG 6 cut(s) 101, 969, 1495, 1754, 2433, 3114
SduI GDGCHC 3 cut(s) 30, 256, 1107
SfaNI GCATC 9 cut(s) 763, 802, 1052, 1480, 2373, 3344, 3505, 3770, 4210
SfcI CTRYAG 6 cut(s) 246, 990, 1648, 2782, 2793, 4651
SfuI TTCGAA 2 cut(s) 946, 4323
SinI GGWCC 1 cut(s) 333
SmiMI CAYNNNNRTG 3 cut(s) 477, 1613, 4565
SmlI CTYRAG 6 cut(s) 212, 1094, 1987, 2213, 3886, 4800
SmoI CTYRAG 6 cut(s) 212, 1094, 1987, 2213, 3886, 4800
SpeI ACTAGT 2 cut(s) 1480, 2026
SphI GCATGC 2 cut(s) 1821, 4151
SsiI CCGC 6 cut(s) 673, 1110, 1927, 2102, 2444, 3712
SspMI CTAG 6 cut(s) 1481, 1850, 2027, 2664, 3510, 4972
StyD4I CCNGG 6 cut(s) 99, 967, 1493, 1752, 2431, 3112
StyI CCWWGG 8 cut(s) 20, 704, 1468, 3321, 3509, 3632, 4224, 4560
TaiI ACGT 5 cut(s) 144, 943, 2285, 3568, 4711
TatI WGTACW 6 cut(s) 186, 1554, 3216, 3274, 3426, 4444
TauI GCSGC 2 cut(s) 676, 2446
TscAI CASTG 9 cut(s) 328, 415, 582, 721, 3072, 3905, 3919, 4066, 4193
TseFI GTSAC 5 cut(s) 1000, 1604, 1768, 2612, 4461
TseI GCWGC 9 cut(s) 1668, 2237, 2645, 2911, 3266, 3402, 3920, 4550, 4874
Tsp45I GTSAC 5 cut(s) 1000, 1604, 1768, 2612, 4461
TspGWI ACGGA 6 cut(s) 58, 101, 2491, 2764, 2889, 4850
TspRI CASTG 9 cut(s) 328, 415, 582, 721, 3072, 3905, 3919, 4066, 4193
Van91I CCANNNNNTGG 1 cut(s) 2273
VpaK11BI GGWCC 1 cut(s) 333
VspI ATTAAT 1 cut(s) 1334
XapI RAATTY 9 cut(s) 872, 1598, 1757, 1912, 3191, 3380, 3740, 4192, 4927
XceI RCATGY 6 cut(s) 844, 1124, 1821, 2234, 2613, 4151
XcmI CCANNNNNNNNNTGG 1 cut(s) 3948
XmaJI CCTAGG 1 cut(s) 3509
XmiI GTMKAC 2 cut(s) 2192, 4657
XmnI GAANNNNTTC 5 cut(s) 162, 942, 2315, 2630, 4593
XspI CTAG 6 cut(s) 1481, 1850, 2027, 2664, 3510, 4972
ZraI GACGTC 1 cut(s) 142
ZrmI AGTACT 2 cut(s) 188, 3276
Zsp2I ATGCAT 3 cut(s) 982, 1511, 3563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.