Serine/Threonine protein kinases, catalytic domain
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| SACS | PF25794 | 30 - 146 | 2.9e-11 | Sacsin domain |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 2349, 2951 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 3053 |
| AatII | GACGTC | 1 cut(s) | 144 |
| Acc65I | GGTACC | 1 cut(s) | 4488 |
| AccB1I | GGYRCC | 1 cut(s) | 4488 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 2270 |
| AccBSI | CCGCTC | 1 cut(s) | 1927 |
| AccI | GTMKAC | 1 cut(s) | 2189 |
| AccII | CGCG | 2 cut(s) | 234, 2197 |
| AccIII | TCCGGA | 1 cut(s) | 1812 |
| AclWI | GGATC | 9 cut(s) | 99, 197, 1445, 2490, 2503, 2803, 4070, 4390, 4515 |
| AcoI | YGGCCR | 1 cut(s) | 2724 |
| AcyI | GRCGYC | 3 cut(s) | 17, 141, 4592 |
| AfeI | AGCGCT | 2 cut(s) | 3357, 4653 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 85, 2084, 2270 |
| AflIII | ACRYGT | 1 cut(s) | 840 |
| AhlI | ACTAGT | 1 cut(s) | 2026 |
| AjnI | CCWGG | 5 cut(s) | 99, 967, 2428, 3109, 3705 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 998, 1030, 4492, 4524 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 1286, 1318, 1401, 1433 |
| Alw21I | GWGCWC | 2 cut(s) | 3912, 4967 |
| Alw26I | GTCTC | 8 cut(s) | 737, 1049, 2506, 2640, 2790, 3125, 3798, 4624 |
| AlwI | GGATC | 9 cut(s) | 99, 197, 1445, 2490, 2503, 2803, 4070, 4390, 4515 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 4046 |
| Ama87I | CYCGRG | 1 cut(s) | 3476 |
| Aor13HI | TCCGGA | 1 cut(s) | 1812 |
| Aor51HI | AGCGCT | 2 cut(s) | 3357, 4653 |
| AoxI | GGCC | 5 cut(s) | 102, 2438, 2724, 3411, 3854 |
| AseI | ATTAAT | 1 cut(s) | 1334 |
| Asp700I | GAANNNNTTC | 5 cut(s) | 162, 942, 2312, 2627, 4725 |
| Asp718I | GGTACC | 1 cut(s) | 4488 |
| AspA2I | CCTAGG | 1 cut(s) | 3641 |
| AspLEI | GCGC | 3 cut(s) | 1159, 3358, 4654 |
| AspS9I | GGNCC | 3 cut(s) | 333, 700, 3854 |
| AsuC2I | CCSGG | 1 cut(s) | 1754 |
| AsuII | TTCGAA | 1 cut(s) | 4455 |
| AsuNHI | GCTAGC | 1 cut(s) | 1849 |
| AvaI | CYCGRG | 1 cut(s) | 3476 |
| AvaII | GGWCC | 2 cut(s) | 333, 700 |
| AvrII | CCTAGG | 1 cut(s) | 3641 |
| AxyI | CCTNAGG | 1 cut(s) | 2997 |
| BaeGI | GKGCMC | 1 cut(s) | 1107 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 4430, 4463 |
| BamHI | GGATCC | 1 cut(s) | 2495 |
| BanI | GGYRCC | 1 cut(s) | 4488 |
| BanII | GRGCYC | 3 cut(s) | 256, 1287, 3912 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1257, 1289 |
| BauI | CACGAG | 1 cut(s) | 1317 |
| Bbv12I | GWGCWC | 2 cut(s) | 3912, 4967 |
| BbvCI | CCTCAGC | 1 cut(s) | 4494 |
| BceAI | ACGGC | 3 cut(s) | 89, 258, 3639 |
| BciT130I | CCWGG | 5 cut(s) | 101, 969, 2430, 3111, 3707 |
| BciVI | GTATCC | 2 cut(s) | 1825, 2549 |
| BclI | TGATCA | 6 cut(s) | 405, 1855, 2857, 3181, 4969, 4995 |
| BcnI | CCSGG | 1 cut(s) | 1754 |
| BcoDI | GTCTC | 8 cut(s) | 737, 1049, 2506, 2640, 2790, 3125, 3798, 4624 |
| BcuI | ACTAGT | 1 cut(s) | 2026 |
| BfaI | CTAG | 6 cut(s) | 1850, 2027, 2661, 3642, 5015, 5104 |
| BfmI | CTRYAG | 4 cut(s) | 246, 1568, 2779, 2790 |
| BfoI | RGCGCY | 2 cut(s) | 3359, 4655 |
| BfuI | GTATCC | 2 cut(s) | 1825, 2549 |
| BglII | AGATCT | 2 cut(s) | 1549, 4863 |
| BlnI | CCTAGG | 1 cut(s) | 3641 |
| BlpI | GCTNAGC | 1 cut(s) | 414 |
| BmcAI | AGTACT | 1 cut(s) | 188 |
| Bme1390I | CCNGG | 6 cut(s) | 101, 969, 1754, 2430, 3111, 3707 |
| Bme18I | GGWCC | 2 cut(s) | 333, 700 |
| BmeT110I | CYCGRG | 1 cut(s) | 3476 |
| BmgT120I | GGNCC | 3 cut(s) | 333, 700, 3854 |
| BmiI | GGNNCC | 5 cut(s) | 255, 702, 2497, 4038, 4490 |
| BmrFI | CCNGG | 6 cut(s) | 101, 969, 1754, 2430, 3111, 3707 |
| BmrI | ACTGGG | 1 cut(s) | 2443 |
| BmtI | GCTAGC | 1 cut(s) | 1853 |
| BmuI | ACTGGG | 1 cut(s) | 2443 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 57, 89 |
| BpmI | CTGGAG | 3 cut(s) | 240, 4174, 4911 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1863, 4494 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 414 |
| Bpu14I | TTCGAA | 1 cut(s) | 4455 |
| BpuEI | CTTGAG | 6 cut(s) | 233, 1115, 1972, 2231, 4039, 5078 |
| BpuMI | CCSGG | 1 cut(s) | 1754 |
| Bsa29I | ATCGAT | 1 cut(s) | 771 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1884 |
| BsaHI | GRCGYC | 3 cut(s) | 17, 141, 4592 |
| BsaI | GGTCTC | 2 cut(s) | 2790, 3798 |
| BsaWI | WCCGGW | 1 cut(s) | 1812 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 238, 268, 1286, 1316 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 85, 2084, 2270 |
| Bse1I | ACTGG | 7 cut(s) | 1277, 1481, 1499, 2449, 2762, 4191, 4325 |
| Bse21I | CCTNAGG | 1 cut(s) | 2997 |
| Bse3DI | GCAATG | 5 cut(s) | 263, 472, 484, 3335, 4422 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1884 |
| BseAI | TCCGGA | 1 cut(s) | 1812 |
| BseBI | CCWGG | 5 cut(s) | 101, 969, 2430, 3111, 3707 |
| BseCI | ATCGAT | 1 cut(s) | 771 |
| BseJI | GATNNNNATC | 1 cut(s) | 1884 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 85, 2084, 2270 |
| BseMI | GCAATG | 5 cut(s) | 263, 472, 484, 3335, 4422 |
| BseNI | ACTGG | 7 cut(s) | 1277, 1481, 1499, 2449, 2762, 4191, 4325 |
| BseRI | GAGGAG | 4 cut(s) | 901, 2579, 4677, 5099 |
| BseSI | GKGCMC | 1 cut(s) | 1107 |
| BseX3I | CGGCCG | 1 cut(s) | 2724 |
| BsgI | GTGCAG | 2 cut(s) | 1687, 2274 |
| Bsh1236I | CGCG | 2 cut(s) | 234, 2197 |
| Bsh1285I | CGRYCG | 1 cut(s) | 2727 |
| BshFI | GGCC | 5 cut(s) | 104, 2440, 2726, 3413, 3856 |
| BshNI | GGYRCC | 1 cut(s) | 4488 |
| BshVI | ATCGAT | 1 cut(s) | 771 |
| BsiEI | CGRYCG | 1 cut(s) | 2727 |
| BsiHKAI | GWGCWC | 2 cut(s) | 3912, 4967 |
| BsiHKCI | CYCGRG | 1 cut(s) | 3476 |
| BsiSI | CCGG | 4 cut(s) | 120, 1753, 1813, 2723 |
| BslFI | GGGAC | 7 cut(s) | 2057, 2115, 2231, 2720, 3379, 3459, 3936 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 85, 2084, 2270 |
| BsmAI | GTCTC | 8 cut(s) | 737, 1049, 2506, 2640, 2790, 3125, 3798, 4624 |
| BsmBI | CGTCTC | 1 cut(s) | 2506 |
| BsmFI | GGGAC | 7 cut(s) | 2057, 2115, 2231, 2720, 3379, 3459, 3936 |
| BsmI | GAATGC | 7 cut(s) | 175, 833, 2314, 2674, 3147, 3462, 3695 |
| BsnI | GGCC | 5 cut(s) | 104, 2440, 2726, 3413, 3856 |
| Bso31I | GGTCTC | 2 cut(s) | 2790, 3798 |
| BsoBI | CYCGRG | 1 cut(s) | 3476 |
| Bsp119I | TTCGAA | 1 cut(s) | 4455 |
| Bsp1286I | GDGCHC | 5 cut(s) | 256, 1107, 1287, 3912, 4967 |
| Bsp13I | TCCGGA | 1 cut(s) | 1812 |
| Bsp1407I | TGTACA | 2 cut(s) | 3976, 4576 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 414 |
| Bsp19I | CCATGG | 2 cut(s) | 3764, 4692 |
| BspANI | GGCC | 5 cut(s) | 104, 2440, 2726, 3413, 3856 |
| BspDI | ATCGAT | 1 cut(s) | 771 |
| BspEI | TCCGGA | 1 cut(s) | 1812 |
| BspFNI | CGCG | 2 cut(s) | 234, 2197 |
| BspHI | TCATGA | 2 cut(s) | 3184, 3744 |
| BspLI | GGNNCC | 5 cut(s) | 255, 702, 2497, 4038, 4490 |
| BspOI | GCTAGC | 1 cut(s) | 1853 |
| BspPI | GGATC | 9 cut(s) | 99, 197, 1445, 2490, 2503, 2803, 4070, 4390, 4515 |
| BspQI | GCTCTTC | 2 cut(s) | 453, 3569 |
| BspT104I | TTCGAA | 1 cut(s) | 4455 |
| BspT107I | GGYRCC | 1 cut(s) | 4488 |
| BspTNI | GGTCTC | 2 cut(s) | 2790, 3798 |
| BsrBI | CCGCTC | 1 cut(s) | 1927 |
| BsrDI | GCAATG | 5 cut(s) | 263, 472, 484, 3335, 4422 |
| BsrGI | TGTACA | 2 cut(s) | 3976, 4576 |
| BsrI | ACTGG | 7 cut(s) | 1277, 1481, 1499, 2449, 2762, 4191, 4325 |
| BssNAI | GTATAC | 1 cut(s) | 2190 |
| BssNI | GRCGYC | 3 cut(s) | 17, 141, 4592 |
| BssSI | CACGAG | 1 cut(s) | 1317 |
| BssT1I | CCWWGG | 9 cut(s) | 20, 704, 1468, 3318, 3452, 3641, 3764, 4356, 4692 |
| Bst1107I | GTATAC | 1 cut(s) | 2190 |
| Bst2BI | CACGAG | 1 cut(s) | 1317 |
| Bst2UI | CCWGG | 5 cut(s) | 101, 969, 2430, 3111, 3707 |
| Bst6I | CTCTTC | 7 cut(s) | 453, 967, 3252, 3569, 4581, 4706, 4895 |
| BstACI | GRCGYC | 3 cut(s) | 17, 141, 4592 |
| BstAUI | TGTACA | 2 cut(s) | 3976, 4576 |
| BstBI | TTCGAA | 1 cut(s) | 4455 |
| BstC8I | GCNNGC | 8 cut(s) | 125, 739, 1341, 1851, 2383, 3652, 3964, 4281 |
| BstDSI | CCRYGG | 3 cut(s) | 2195, 3764, 4692 |
| BstEII | GGTNACC | 1 cut(s) | 1000 |
| BstFNI | CGCG | 2 cut(s) | 234, 2197 |
| BstH2I | RGCGCY | 2 cut(s) | 3359, 4655 |
| BstHHI | GCGC | 3 cut(s) | 1159, 3358, 4654 |
| BstMAI | GTCTC | 8 cut(s) | 737, 1049, 2506, 2640, 2790, 3125, 3798, 4624 |
| BstMCI | CGRYCG | 1 cut(s) | 2727 |
| BstNI | CCWGG | 5 cut(s) | 101, 969, 2430, 3111, 3707 |
| BstNSI | RCATGY | 6 cut(s) | 755, 844, 1821, 2231, 2610, 4283 |
| BstPI | GGTNACC | 1 cut(s) | 1000 |
| BstSCI | CCNGG | 6 cut(s) | 99, 967, 1752, 2428, 3109, 3705 |
| BstSFI | CTRYAG | 4 cut(s) | 246, 1568, 2779, 2790 |
| BstSLI | GKGCMC | 1 cut(s) | 1107 |
| BstUI | CGCG | 2 cut(s) | 234, 2197 |
| BstX2I | RGATCY | 8 cut(s) | 91, 1549, 2495, 2795, 4062, 4382, 4520, 4863 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 268, 997, 4699 |
| BstYI | RGATCY | 8 cut(s) | 91, 1549, 2495, 2795, 4062, 4382, 4520, 4863 |
| BstZ17I | GTATAC | 1 cut(s) | 2190 |
| BstZI | CGGCCG | 1 cut(s) | 2724 |
| Bsu15I | ATCGAT | 1 cut(s) | 771 |
| Bsu36I | CCTNAGG | 1 cut(s) | 2997 |
| BsuI | GTATCC | 2 cut(s) | 1825, 2549 |
| BsuRI | GGCC | 5 cut(s) | 104, 2440, 2726, 3413, 3856 |
| BsuTUI | ATCGAT | 1 cut(s) | 771 |
| BtgI | CCRYGG | 3 cut(s) | 2195, 3764, 4692 |
| BtsI | GCAGTG | 2 cut(s) | 328, 408 |
| Cac8I | GCNNGC | 8 cut(s) | 125, 739, 1341, 1851, 2383, 3652, 3964, 4281 |
| CaiI | CAGNNNCTG | 1 cut(s) | 4046 |
| CciI | TCATGA | 2 cut(s) | 3184, 3744 |
| CfoI | GCGC | 3 cut(s) | 1159, 3358, 4654 |
| Cfr13I | GGNCC | 3 cut(s) | 333, 700, 3854 |
| Cfr42I | CCGCGG | 1 cut(s) | 2198 |
| ClaI | ATCGAT | 1 cut(s) | 771 |
| CseI | GACGC | 3 cut(s) | 25, 744, 4581 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 3053 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 3053 |
| EaeI | YGGCCR | 1 cut(s) | 2724 |
| EagI | CGGCCG | 1 cut(s) | 2724 |
| Eam1104I | CTCTTC | 7 cut(s) | 453, 967, 3252, 3569, 4581, 4706, 4895 |
| EarI | CTCTTC | 7 cut(s) | 453, 967, 3252, 3569, 4581, 4706, 4895 |
| EciI | GGCGGA | 1 cut(s) | 1043 |
| Ecl136II | GAGCTC | 1 cut(s) | 3910 |
| EclXI | CGGCCG | 1 cut(s) | 2724 |
| Eco130I | CCWWGG | 9 cut(s) | 20, 704, 1468, 3318, 3452, 3641, 3764, 4356, 4692 |
| Eco24I | GRGCYC | 3 cut(s) | 256, 1287, 3912 |
| Eco31I | GGTCTC | 2 cut(s) | 2790, 3798 |
| Eco32I | GATATC | 1 cut(s) | 4941 |
| Eco47I | GGWCC | 2 cut(s) | 333, 700 |
| Eco47III | AGCGCT | 2 cut(s) | 3357, 4653 |
| Eco52I | CGGCCG | 1 cut(s) | 2724 |
| Eco53kI | GAGCTC | 1 cut(s) | 3910 |
| Eco81I | CCTNAGG | 1 cut(s) | 2997 |
| Eco88I | CYCGRG | 1 cut(s) | 3476 |
| Eco91I | GGTNACC | 1 cut(s) | 1000 |
| EcoICRI | GAGCTC | 1 cut(s) | 3910 |
| EcoO109I | RGGNCCY | 1 cut(s) | 700 |
| EcoO65I | GGTNACC | 1 cut(s) | 1000 |
| EcoRI | GAATTC | 6 cut(s) | 872, 1912, 2164, 3188, 3872, 4324 |
| EcoRII | CCWGG | 5 cut(s) | 99, 967, 2428, 3109, 3705 |
| EcoRV | GATATC | 1 cut(s) | 4941 |
| EcoT14I | CCWWGG | 9 cut(s) | 20, 704, 1468, 3318, 3452, 3641, 3764, 4356, 4692 |
| EcoT22I | ATGCAT | 4 cut(s) | 982, 1511, 3301, 3695 |
| EcoT38I | GRGCYC | 3 cut(s) | 256, 1287, 3912 |
| ErhI | CCWWGG | 9 cut(s) | 20, 704, 1468, 3318, 3452, 3641, 3764, 4356, 4692 |
| Esp3I | CGTCTC | 1 cut(s) | 2506 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 946, 978, 1077, 1109, 3815, 3847, 4492, 4524 |
| FaqI | GGGAC | 7 cut(s) | 2057, 2115, 2231, 2720, 3379, 3459, 3936 |
| FauI | CCCGC | 1 cut(s) | 2092 |
| FauNDI | CATATG | 1 cut(s) | 1945 |
| FbaI | TGATCA | 6 cut(s) | 405, 1855, 2857, 3181, 4969, 4995 |
| FblI | GTMKAC | 1 cut(s) | 2189 |
| FriOI | GRGCYC | 3 cut(s) | 256, 1287, 3912 |
| FspBI | CTAG | 6 cut(s) | 1850, 2027, 2661, 3642, 5015, 5104 |
| GlaI | GCGC | 3 cut(s) | 1158, 3357, 4653 |
| GsuI | CTGGAG | 3 cut(s) | 240, 4174, 4911 |
| HaeII | RGCGCY | 2 cut(s) | 3359, 4655 |
| HaeIII | GGCC | 5 cut(s) | 104, 2440, 2726, 3413, 3856 |
| HapII | CCGG | 4 cut(s) | 120, 1753, 1813, 2723 |
| HgaI | GACGC | 3 cut(s) | 25, 744, 4581 |
| HhaI | GCGC | 3 cut(s) | 1159, 3358, 4654 |
| Hin1I | GRCGYC | 3 cut(s) | 17, 141, 4592 |
| Hin6I | GCGC | 3 cut(s) | 1157, 3356, 4652 |
| HinP1I | GCGC | 3 cut(s) | 1157, 3356, 4652 |
| HincII | GTYRAC | 4 cut(s) | 2002, 2339, 2509, 4143 |
| HindII | GTYRAC | 4 cut(s) | 2002, 2339, 2509, 4143 |
| HindIII | AAGCTT | 5 cut(s) | 1384, 1394, 1931, 2204, 3993 |
| HpaII | CCGG | 4 cut(s) | 120, 1753, 1813, 2723 |
| Hpy166II | GTNNAC | 9 cut(s) | 145, 336, 470, 2002, 2190, 2339, 2509, 4143, 4558 |
| Hpy8I | GTNNAC | 9 cut(s) | 145, 336, 470, 2002, 2190, 2339, 2509, 4143, 4558 |
| Hpy99I | CGWCG | 3 cut(s) | 10, 13, 3596 |
| HpyCH4IV | ACGT | 2 cut(s) | 141, 940 |
| HpySE526I | ACGT | 2 cut(s) | 141, 940 |
| Hsp92I | GRCGYC | 3 cut(s) | 17, 141, 4592 |
| HspAI | GCGC | 3 cut(s) | 1157, 3356, 4652 |
| Kpn2I | TCCGGA | 1 cut(s) | 1812 |
| KpnI | GGTACC | 1 cut(s) | 4492 |
| Ksp22I | TGATCA | 6 cut(s) | 405, 1855, 2857, 3181, 4969, 4995 |
| KspI | CCGCGG | 1 cut(s) | 2198 |
| LguI | GCTCTTC | 2 cut(s) | 453, 3569 |
| LmnI | GCTCC | 8 cut(s) | 259, 1294, 2266, 2566, 3198, 3396, 4036, 4690 |
| MaeI | CTAG | 6 cut(s) | 1850, 2027, 2661, 3642, 5015, 5104 |
| MaeII | ACGT | 2 cut(s) | 141, 940 |
| MbiI | CCGCTC | 1 cut(s) | 1927 |
| MfeI | CAATTG | 3 cut(s) | 726, 930, 2577 |
| MflI | RGATCY | 8 cut(s) | 91, 1549, 2495, 2795, 4062, 4382, 4520, 4863 |
| MhlI | GDGCHC | 5 cut(s) | 256, 1107, 1287, 3912, 4967 |
| MlyI | GAGTC | 8 cut(s) | 305, 1247, 1765, 3081, 3361, 3769, 3812, 4481 |
| MmeI | TCCRAC | 2 cut(s) | 307, 590 |
| Mph1103I | ATGCAT | 4 cut(s) | 982, 1511, 3301, 3695 |
| MroI | TCCGGA | 1 cut(s) | 1812 |
| MroXI | GAANNNNTTC | 5 cut(s) | 162, 942, 2312, 2627, 4725 |
| MslI | CAYNNNNRTG | 5 cut(s) | 477, 995, 1613, 3972, 4697 |
| MspA1I | CMGCKG | 3 cut(s) | 803, 2197, 4763 |
| MspI | CCGG | 4 cut(s) | 120, 1753, 1813, 2723 |
| MspR9I | CCNGG | 6 cut(s) | 101, 969, 1754, 2430, 3111, 3707 |
| MunI | CAATTG | 3 cut(s) | 726, 930, 2577 |
| Mva1269I | GAATGC | 7 cut(s) | 175, 833, 2314, 2674, 3147, 3462, 3695 |
| MvaI | CCWGG | 5 cut(s) | 101, 969, 2430, 3111, 3707 |
| MvnI | CGCG | 2 cut(s) | 234, 2197 |
| NciI | CCSGG | 1 cut(s) | 1754 |
| NcoI | CCATGG | 2 cut(s) | 3764, 4692 |
| NdeI | CATATG | 1 cut(s) | 1945 |
| NheI | GCTAGC | 1 cut(s) | 1849 |
| NlaIV | GGNNCC | 5 cut(s) | 255, 702, 2497, 4038, 4490 |
| NmeAIII | GCCGAG | 1 cut(s) | 4653 |
| NmuCI | GTSAC | 5 cut(s) | 1000, 1604, 1768, 2609, 4593 |
| NsiI | ATGCAT | 4 cut(s) | 982, 1511, 3301, 3695 |
| NspI | RCATGY | 6 cut(s) | 755, 844, 1821, 2231, 2610, 4283 |
| NspV | TTCGAA | 1 cut(s) | 4455 |
| PaeI | GCATGC | 1 cut(s) | 4283 |
| PagI | TCATGA | 2 cut(s) | 3184, 3744 |
| PciI | ACATGT | 1 cut(s) | 840 |
| PciSI | GCTCTTC | 2 cut(s) | 453, 3569 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 138, 2046 |
| PctI | GAATGC | 7 cut(s) | 175, 833, 2314, 2674, 3147, 3462, 3695 |
| PdmI | GAANNNNTTC | 5 cut(s) | 162, 942, 2312, 2627, 4725 |
| PflFI | GACNNNGTC | 1 cut(s) | 2048 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 2270 |
| PfoI | TCCNGGA | 1 cut(s) | 3705 |
| PleI | GAGTC | 8 cut(s) | 305, 1246, 1765, 3080, 3361, 3769, 3811, 4480 |
| PpsI | GAGTC | 8 cut(s) | 305, 1246, 1765, 3080, 3361, 3769, 3811, 4480 |
| PpuMI | RGGWCCY | 1 cut(s) | 700 |
| PscI | ACATGT | 1 cut(s) | 840 |
| PshBI | ATTAAT | 1 cut(s) | 1334 |
| PsiI | TTATAA | 2 cut(s) | 2349, 2951 |
| Psp124BI | GAGCTC | 1 cut(s) | 3912 |
| Psp5II | RGGWCCY | 1 cut(s) | 700 |
| Psp6I | CCWGG | 5 cut(s) | 99, 967, 2428, 3109, 3705 |
| PspEI | GGTNACC | 1 cut(s) | 1000 |
| PspGI | CCWGG | 5 cut(s) | 99, 967, 2428, 3109, 3705 |
| PspN4I | GGNNCC | 5 cut(s) | 255, 702, 2497, 4038, 4490 |
| PspPI | GGNCC | 3 cut(s) | 333, 700, 3854 |
| PspPPI | RGGWCCY | 1 cut(s) | 700 |
| PstNI | CAGNNNCTG | 1 cut(s) | 4046 |
| PsuI | RGATCY | 8 cut(s) | 91, 1549, 2495, 2795, 4062, 4382, 4520, 4863 |
| PsyI | GACNNNGTC | 1 cut(s) | 2048 |
| PvuII | CAGCTG | 1 cut(s) | 4763 |
| RseI | CAYNNNNRTG | 5 cut(s) | 477, 995, 1613, 3972, 4697 |
| SacI | GAGCTC | 1 cut(s) | 3912 |
| SacII | CCGCGG | 1 cut(s) | 2198 |
| SapI | GCTCTTC | 2 cut(s) | 453, 3569 |
| Sau96I | GGNCC | 3 cut(s) | 333, 700, 3854 |
| ScaI | AGTACT | 1 cut(s) | 188 |
| SchI | GAGTC | 8 cut(s) | 305, 1247, 1765, 3081, 3361, 3769, 3812, 4481 |
| ScrFI | CCNGG | 6 cut(s) | 101, 969, 1754, 2430, 3111, 3707 |
| SduI | GDGCHC | 5 cut(s) | 256, 1107, 1287, 3912, 4967 |
| SfcI | CTRYAG | 4 cut(s) | 246, 1568, 2779, 2790 |
| Sfr303I | CCGCGG | 1 cut(s) | 2198 |
| SfuI | TTCGAA | 1 cut(s) | 4455 |
| SgrBI | CCGCGG | 1 cut(s) | 2198 |
| SinI | GGWCC | 2 cut(s) | 333, 700 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 477, 995, 1613, 3972, 4697 |
| SmlI | CTYRAG | 6 cut(s) | 212, 1094, 1987, 2210, 4018, 5093 |
| SmoI | CTYRAG | 6 cut(s) | 212, 1094, 1987, 2210, 4018, 5093 |
| SpeI | ACTAGT | 1 cut(s) | 2026 |
| SphI | GCATGC | 1 cut(s) | 4283 |
| SspI | AATATT | 2 cut(s) | 307, 1721 |
| SspMI | CTAG | 6 cut(s) | 1850, 2027, 2661, 3642, 5015, 5104 |
| SstI | GAGCTC | 1 cut(s) | 3912 |
| StyD4I | CCNGG | 6 cut(s) | 99, 967, 1752, 2428, 3109, 3705 |
| StyI | CCWWGG | 9 cut(s) | 20, 704, 1468, 3318, 3452, 3641, 3764, 4356, 4692 |
| TaiI | ACGT | 2 cut(s) | 144, 943 |
| TatI | WGTACW | 5 cut(s) | 186, 1554, 3558, 3976, 4576 |
| TauI | GCSGC | 1 cut(s) | 676 |
| TseFI | GTSAC | 5 cut(s) | 1000, 1604, 1768, 2609, 4593 |
| Tsp45I | GTSAC | 5 cut(s) | 1000, 1604, 1768, 2609, 4593 |
| TspGWI | ACGGA | 4 cut(s) | 58, 101, 2488, 2761 |
| Tth111I | GACNNNGTC | 1 cut(s) | 2048 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 2270 |
| VpaK11BI | GGWCC | 2 cut(s) | 333, 700 |
| VspI | ATTAAT | 1 cut(s) | 1334 |
| XceI | RCATGY | 6 cut(s) | 755, 844, 1821, 2231, 2610, 4283 |
| XmaJI | CCTAGG | 1 cut(s) | 3641 |
| XmiI | GTMKAC | 1 cut(s) | 2189 |
| XmnI | GAANNNNTTC | 5 cut(s) | 162, 942, 2312, 2627, 4725 |
| XspI | CTAG | 6 cut(s) | 1850, 2027, 2661, 3642, 5015, 5104 |
| ZraI | GACGTC | 1 cut(s) | 142 |
| ZrmI | AGTACT | 1 cut(s) | 188 |
| Zsp2I | ATGCAT | 4 cut(s) | 982, 1511, 3301, 3695 |