Rorug06G0088500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000526
Physical Location & Seq
Reverse (-)
11810201 .. 11822047
11847 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0088500.1

Sequence Viewer

Length: 4527 bp
ATGTTGCCCCTGCAGCCAGTTGAAACCAATCTTAAAGCAACTTTAGCTGGGATTTCTGTTGTATTTCCATTCCAAGATGAAAACCAGAATCATTTGTGTGATACAAAGGGTGATTTAGGTTCTAGTTCAGATGTTCTATATCTATGTGTAGAATGCCGAGATATCCTTCTTGCTATGCAGGTGTCTTCTCGGCATATGAGGTTTGAAGGAACAATGGACTATATTGAGGTTGCCAATTACGCAAGTCATAAAGACGGTACCTTGGAGTTGGGTTTGCAAGGGTGTAACAACAAGGTTAAAAGCCAAACTTCTTCAATTCAACATCTTCAAGCCGATGTTCAAAGTGTTCTTCCTTTGCATGCCTCATCTTATTATTCAGCTGAATCAAATGGTTTAGCTGCTGAAGGTTTTCCATTTGGATATAGGGATGATTTAGTCAGAATTACATTGCTTAAAACTTCAGGTGTCACTTATTGTCAATGTACTGTCAGTTCTAGTTCATCTGATGGGAGTTCAACTGGGCCTACATCATTTTCACTGAAACTGCCACACTTTGTTTTCTGGGTGGACTTCTCTTTGCTAAATATTCTGTTAGAACTGTTGAAGGAAATTGGAAAGACTGTTGAAGTGAATGACCAGGCCGAGTTTTCTTCGGAGGCCTCTAATAAGATCCATGGATCATCTCATAGGGACCTTAGAAGAGCTTCTAGTTGTGTTACAACCTTGTCTTCAACAGATAGTGTGCGAGGTGATATATTTATCCCTAATGCACGGATAATTCTTTGTTTACGTTCCAATGGTGGTGAAACTGTTAGAAGCTTCTCCTCCTGGGATCAATTTGTTGCTCTGGAATTTACTTCACCTTCGACTTGTGACAAAGGCATGATTCAAGACCATGGTCCTACCTTTGATGCAAGTTCAGAGAAAAGGTATTCTTCAACTGTCACACGTTCTTTACAGTTGAATGTTGGTGATCTTGATGTTTTCCTGGTCAATCCTCGAAGTAAAGATAATGCAGAAATCAGATCTGGCAAGATGCAGAGGCTAACACTTACTGCACAAAAAGTAATGTCTGTCAGCAACAGAAAAGGCAGTCTTTCTGTCATTAGTATGCTTTGGCAAGAGGGTTATGTGACTGGTCCTTGGATAGCAAAGAAAGCTAAGTGCCTCGCTACTTTTGAGGAATCAAAGAGCATTTCCAAATTTGTGGGAAAAGATCACGAGTTTGCTTCTGTATCTACTGTGAAAGATCTTAAGGATTTAAGCTCCCAGACACGACAAGAGATTATTTTGAGCTCTGCATTTTTCCTACATGTTTGTCTACCTGCTGTTACAATAAAGTTAGACAATCCTCAATATAAAGAGTTGTGTCATCTTTTGGATGAAGTGATGAATGACATATCTGGTGTGGATCTCGATTCTATCAATGATAAGGCAGAATCTTCCATGCCTCAGACATCTATCCTTGTGGACTGTGATTCTGTTGAAATATTGATTAACTTGGATGTGAAGGAGACTGTCCCAGGCTCAATGCAGAGTGAACTTCCTGGGACGTGGAATCGTCTAAGACTCAAAGTTCAGAAGCTTGAAATGCTATCTGTTTCGAATATTGGAGGTATTCCAGGTGCCACTTTTTTCTGGCTGGCACACGGTGAAGGCAAATTGTGGGGTTCTATCACTAGTATTCCTGATCAGGAGTTTCTTCTGATCACATGTAGCAACTCGACAATGAAACGTGGCGATGGAGGAGGTTCAAATGCATTATCATCGATATTTGCTGGTTCTGATATTGTACATCTGTGGGATCCTACAGGTTTTCATGGTTCTACATCTATAACAGTCAGATGTGCCACAATTGTTGCTGTTGGGGGTCGCTTGGATTGGCCGGATGCACTATGCTCCTTTTTCATTATTCCTTCTGAAATTGAACAAGCAGAGGACAAGTGTAATCAAAAGGACGATGCACCTCGTGGATCTTCTTTTGTGCTTAACTTGGTTGATATTGGATTAAGCTATGAACCCTACCAAAAGAATATGGTGGTCAGGAGTGAAGACTCAGGGTCCAGTTGTTCGTCTGTCAAAGAGACATGTGAAGAATATGTTTCTTGTCTTTTGGCTGCATCTTCCTTGAATCTTTCAACTTCAACCGTGGGAGATTCCACAGAAATGAATTACAAAATTAGAGTGCGAGATCTTGGGCTTCTTCTCCGTGTATTGTTAAAGCCTGAGGATATTGGTGGCACTTACAGTGCAGAGCATCTTCACAAGATTGGGTATGTTAAAGTTGCAAGGGAGGCACTCGTTGAAGCAAATTTGAGAACTAATTGTAAAAATGGTCTTCTCTGGGAGGTAGAATGTTCAAAGTCCCACATTTTTGTGGAAACATGCCATGACACAATGTCTAGTCTGATTCGTTTGGCTGCTCAAATCCAACAGCTATTTGCTCCTGACCTGGAGGAATCAATTGTGCATTTGCAGACAAGGTGGAATAAATTTCAGCAGGAACAAGAGAGGAGAGGTTTGGCTAATGAAATCAGGATATTCGATAGTGAGTCTCCAACTGCTCAATTACACACGTCTGGTCTAGTTACAGAGAGAGAGCCTCAGTTGGTTGGGTTAATGGATGAGATATCAGAAGATGCATTTCGAGATAATAATCACACCTACCAATATGACTCTTCTGAGTCACAAATTGGTATATCATCTGATGAGGACCTTGGAGAGGCGTGTTACTCACGTATTGGAACTCCTGATATTTTCTTGCCTGGTACATCTTATGATGGGTCAGTGCCATCAGTTGAATCAGAAAGTAGTCAAACATCATTCCTACAAGAAGGTAATGTTTTGGAGTTAATAGAAGGGTATTGTTTATCTGAGTTACGACGACCTCTCTCGGAATTGTCTGTTGGCAGGCAATCATCTCAGGAGATTGTGAAATCCCAGTCCAAGAATGCCAGAATTGGAGATGGGTCAAGAGAAAATCATGGATGGTATGGCACCTCCGTAAAAATTTTAGAAAATCATATTCCAGAAACGAGTGAAAGTGGTAAGGAACAATTTGTCGAAGACAAGCTTCCTTCCACTGGCATTACAAATTGCAATGATCTAGGGAAAGCAATTGGACGTGTACTCCTTAAGAACATTGATGTTAGATGGAGAATGCTTGCTGGCTCTGACTGGCATGATTCTAGAGCAACTGGTCAGCGGTCGGGGGATTTTACTGGAAGGGATGCTACCGTATGCCTGGAGTTCTCACTATGTGGGATGGAATTTCAATATGACGTTTTCCCAGTTGGTGGAATATGTGTATCCAAGCTTTCTCTTTCGGTTCAAGACTTTTATCTATATGATAAGAGCAAAGATGCTCCTTGGAAACTGGTGCTAGGACATTATCATTCAAAGGATCATCCTCGGAAATCATCTTCAAAAGGATTCAAGCTGGACTTAGAAGCGGTCAGACCAGATCCTCTGACACCTCTCGAAGAATATAGGTTGCGAGTCGCATTCCTTCCCATGTTGTTACATCTTCACCAGTGCCAGCTTGATTTTCTCATTGGCTTCTTTGGCCCAAAGAGCTCTTCAGTTGATCAGTCTTCAGGTTGTTATCAAGATCCAGATGGTTCAAAAGTGTTGCCAACAAAGAGTAATAATCTTGCAGGGCATGACATTGCAGAGGAGGCATTTCTTCCTTACTTTCAGAAGTTCGATATATGGCCTATTCTTGTTAGGGTGGACTACAGTCCCAGTCGTGTTGATCTGGCAGCATTAAGAGGCGGGAAGTATGTAGAACTTGTAAATATTGTCCCATGGAAGGGGGTCGAGCTACAGCTTAAACGTGTTCATGCCGTTGGTATTTATGGCTGGGGTAGTGTATGTGAAACAATTATAGGGGAGTGGTTAGAAGACATCTCTCAAAATCAGATTCATAAAATATTGCAAGGTCTTCCTACCATTCGATCATTGGTTGCTGTTGGTGCCGGTGCAGCAAAGCTGGTTTCTTTGCCAGTTGAGCACTATAGGAAGGACAAGAGGGTAGTCAAGGGAATGCAAAGAGGTACAATTGCATTTCTTAGAAGTATTTCTCTTGAAGCTGTTGGGCTTGGAGTACATTTAGCAGCTGGGGCTCATGATATTTTGCTCCAAGCTGAATATTTGCTTACAAGCATTCCTCCTTCTGTACCCTGGTCAGCACCACATAGAGTAAAATCCAGTGCTAGATCCAATCAACCTAAAGATGCCCAACAAGGAATTCATCAGGCCTATGAAAGTCTCAGTGATGGCTTGGGGAAATCTGCTTCTGCCTTGGTTCGGACGCCCCTGAAAAAATATCAGCGTGGAGCTGGTGCTGGGTCAGCTCTGGCATCTGCTGTTCGGGCAGTCCCTGCAGCTGCTATAGCTCCAGCCTCTGCATGTGCAAGTGCTGTACATTGTGCTCTTCTTGGCTTCAGAAATAGCCTTGATCCTGAGCGTAAGAAAGAGTCTATGGAGAAATATCTAGGCCCCCCTCAGCCATGGGAACAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1508

Amino Acids

165.73

Weight (kDa)

5.63

Isoelectric Point (pI)

46.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG2_CAD PF13329 79 - 1066 7.2e-25 Autophagy-related protein 2
ATG2_CAD PF13329 1076 - 1498 1.4e-128 Autophagy-related protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28020 AT3G48770 AT3G48770
fragaria_vesca FvH4_5g09020 FvH4_5g09020
malus_domestica MD06G1092400.v1.1 MD06G1134600.v1.1 MD06G1134700.v1.1 MD06G1134800.v1.1 MD06G1135200.v1.1 MD06G1135400.v1.1 MD06G1135500.v1.1 MD08G1137600.v1.1 MD15G1395100.v1.1
prunus_persica Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145600_v2.0.a1 Prupe.5G145800_v2.0.a1 Prupe.5G145900_v2.0.a1
pyrus_communis pycom06g12550 pycom06g12570 pycom06g12580 pycom06g12590 pycom06g12610 pycom06g12630 pycom06g12640 pycom06g12650 pycom08g06280
rosa_chinensis RchiOBHm_Chr6g0249321 RchiOBHm_Chr6g0275111 RchiOBHm_Chr7g0189191 RchiOBHm_Chr7g0189261
rosa_laevigata RLG00000004622 RLG00000004623 RLG00000008237 RLG00000008238 RLG00000023303 RLG00000035187
rosa_multiflora Rmu_co8294311.1_g000001 Rmu_co8389833.1_g000001 Rmu_sc0001044.1_g000004 Rmu_sc0001329.1_g000011 Rmu_sc0006012.1_g000010 Rmu_sc0007286.1_g000032 Rmu_sc0009360.1_g000016 Rmu_sc0011817.1_g000001 Rmu_sc0015050.1_g000001
rosa_roxburghii Rroxscaffold_1G00064000 Rroxscaffold_3G00265460 Rroxscaffold_3G00265580 Rroxscaffold_3G00265640 Rroxscaffold_3G00265690 Rroxscaffold_3G00265700 Rroxscaffold_7G00193620 Rroxscaffold_7G00204550 Rroxscaffold_7G00217350
rosa_rugosa Rorug01G0465500 Rorug06G0088500 Rorug06G0496700 Rorug06G0496700
rosa_samantha Rh1AG064800 Rh2BG015100 Rh2BG493500 Rh2DG017300 Rh2DG502500 Rh4BG208500 Rh5BG528500 Rh5CG552900 Rh6CG207800 Rh6DG197900 Rh6DG198000 Rh7AG101300 Rh7AG101400 Rh7AG101600 Rh7BG104100 Rh7BG104200 Rh7CG105600 Rh7CG105700 Rh7DG103200 Rh7DG103400
rosa_wichuraiana Rw1G007300 Rw5G034670 Rw7G008750 Rw7G008780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 169
Acc36I ACCTGC 2 cut(s) 169, 1333
Acc65I GGTACC 1 cut(s) 257
AccB1I GGYRCC 4 cut(s) 257, 1625, 2996, 3974
AccB7I CCANNNNNTGG 1 cut(s) 3296
AccI GTMKAC 1 cut(s) 1321
AciI CCGC 3 cut(s) 3205, 3452, 3774
AcoI YGGCCR 1 cut(s) 1883
AcsI RAATTY 7 cut(s) 851, 1202, 2311, 2492, 3009, 3269, 4248
AcuI CTGAAG 5 cut(s) 423, 444, 3564, 3579, 4429
AcyI GRCGYC 1 cut(s) 4313
AdeI CACNNNGTG 3 cut(s) 1652, 1970, 3260
AfaI GTAC 9 cut(s) 259, 484, 1794, 2770, 3129, 4057, 4107, 4179, 4425
AfiI CCNNNNNNNGG 6 cut(s) 2722, 3083, 3296, 3811, 3812, 4308
AflII CTTAAG 2 cut(s) 1253, 3134
AflIII ACRYGT 7 cut(s) 947, 1312, 1712, 2087, 2574, 3124, 3835
AhdI GACNNNNNGTC 2 cut(s) 2059, 2398
AhlI ACTAGT 1 cut(s) 1679
AjiI CACGTC 3 cut(s) 1554, 2577, 3125
AjuI GAANNNNNNNTTGG 6 cut(s) 2889, 2921, 3562, 3594, 4233, 4265
Alw21I GWGCWC 4 cut(s) 1298, 3578, 4014, 4435
Alw26I GTCTC 4 cut(s) 1508, 2078, 2559, 4274
AlwNI CAGNNNCTG 2 cut(s) 4382, 4406
AoxI GGCC 8 cut(s) 521, 639, 657, 1883, 3565, 3713, 4257, 4498
ApeKI GCWGC 9 cut(s) 13, 398, 2117, 2420, 3761, 3983, 4115, 4385, 4388
ApoI RAATTY 7 cut(s) 851, 1202, 2311, 2492, 3009, 3269, 4248
Asp700I GAANNNNTTC 3 cut(s) 408, 703, 4078
Asp718I GGTACC 1 cut(s) 257
AspS9I GGNCC 8 cut(s) 521, 691, 899, 1139, 2061, 2713, 3566, 4499
AsuHPI GGTGA 7 cut(s) 122, 761, 815, 852, 983, 1664, 3521
AsuII TTCGAA 1 cut(s) 1604
AvaII GGWCC 5 cut(s) 691, 899, 1139, 2061, 2713
AxyI CCTNAGG 1 cut(s) 2226
BamHI GGATCC 1 cut(s) 1804
BanI GGYRCC 4 cut(s) 257, 1625, 2996, 3974
BanII GRGCYC 3 cut(s) 1298, 3578, 4126
BarI GAAGNNNNNNTAC 2 cut(s) 3217, 3249
BauI CACGAG 2 cut(s) 1220, 1968
BbsI GAAGAC 8 cut(s) 177, 720, 2058, 2330, 3072, 3585, 3909, 3935
Bbv12I GWGCWC 4 cut(s) 1298, 3578, 4014, 4435
BbvCI CCTCAGC 1 cut(s) 4506
BbvI GCAGC 9 cut(s) 25, 385, 2104, 2407, 3773, 3995, 4127, 4375, 4397
BceAI ACGGC 1 cut(s) 3830
BcgI CGANNNNNNTGC 2 cut(s) 1749, 1783
BciVI GTATCC 1 cut(s) 3319
BclI TGATCA 3 cut(s) 1690, 1707, 3586
BcoDI GTCTC 4 cut(s) 1508, 2078, 2559, 4274
BcuI ACTAGT 1 cut(s) 1679
BfmI CTRYAG 7 cut(s) 11, 1809, 3736, 3824, 4015, 4383, 4392
BfrI CTTAAG 2 cut(s) 1253, 3134
BfuAI ACCTGC 2 cut(s) 169, 1333
BfuI GTATCC 1 cut(s) 3319
BglII AGATCT 3 cut(s) 1025, 1249, 2191
BisI GCNGC 9 cut(s) 14, 399, 2118, 2421, 3762, 3984, 4116, 4386, 4389
BlsI GCNGC 9 cut(s) 15, 400, 2119, 2422, 3763, 3985, 4117, 4387, 4390
Bme18I GGWCC 5 cut(s) 691, 899, 1139, 2061, 2713
BmeRI GACNNNNNGTC 2 cut(s) 2059, 2398
BmgBI CACGTC 3 cut(s) 1554, 2577, 3125
BmgT120I GGNCC 8 cut(s) 521, 691, 899, 1139, 2061, 2713, 3566, 4499
BmiI GGNNCC 8 cut(s) 259, 692, 1627, 1806, 2062, 2998, 3976, 4501
BmrI ACTGGG 4 cut(s) 528, 2935, 3284, 3738
BmuI ACTGGG 4 cut(s) 528, 2935, 3284, 3738
BoxI GACNNNNGTC 2 cut(s) 897, 3738
BpiI GAAGAC 8 cut(s) 177, 720, 2058, 2330, 3072, 3585, 3909, 3935
BplI GAGNNNNNCTC 2 cut(s) 2587, 2619
BpmI CTGGAG 3 cut(s) 2474, 3266, 4383
Bpu10I CCTNAGC 2 cut(s) 4464, 4506
Bpu14I TTCGAA 1 cut(s) 1604
Bsa29I ATCGAT 1 cut(s) 1769
BsaAI YACGTR 1 cut(s) 2738
BsaBI GATNNNNATC 1 cut(s) 2655
BsaHI GRCGYC 1 cut(s) 4313
BsaXI ACNNNNNCTCC 2 cut(s) 3114, 3144
Bsc4I CCNNNNNNNGG 6 cut(s) 2722, 3083, 3296, 3811, 3812, 4308
Bse118I RCCGGY 1 cut(s) 3977
Bse21I CCTNAGG 1 cut(s) 2226
Bse3DI GCAATG 3 cut(s) 446, 3106, 3666
Bse8I GATNNNNATC 1 cut(s) 2655
BseCI ATCGAT 1 cut(s) 1769
BseGI GGATG 9 cut(s) 433, 1387, 1510, 1894, 2629, 2993, 3235, 3270, 3406
BseJI GATNNNNATC 1 cut(s) 2655
BseLI CCNNNNNNNGG 6 cut(s) 2722, 3083, 3296, 3811, 3812, 4308
BseMI GCAATG 3 cut(s) 446, 3106, 3666
BseRI GAGGAG 4 cut(s) 814, 1761, 2527, 3689
BseXI GCAGC 9 cut(s) 25, 385, 2104, 2407, 3773, 3995, 4127, 4375, 4397
BseYI CCCAGC 4 cut(s) 47, 3861, 4118, 4346
BsgI GTGCAG 3 cut(s) 1041, 2271, 4002
Bsh1285I CGRYCG 1 cut(s) 3209
BshFI GGCC 8 cut(s) 523, 641, 659, 1885, 3567, 3715, 4259, 4500
BshNI GGYRCC 4 cut(s) 257, 1625, 2996, 3974
BshVI ATCGAT 1 cut(s) 1769
BsiEI CGRYCG 1 cut(s) 3209
BsiHKAI GWGCWC 4 cut(s) 1298, 3578, 4014, 4435
BsiSI CCGG 2 cut(s) 1886, 3978
BslFI GGGAC 7 cut(s) 704, 1505, 1564, 2350, 3726, 3788, 4364
BslI CCNNNNNNNGG 6 cut(s) 2722, 3083, 3296, 3811, 3812, 4308
BsmAI GTCTC 4 cut(s) 1508, 2078, 2559, 4274
BsmFI GGGAC 7 cut(s) 704, 1505, 1564, 2350, 3726, 3788, 4364
BsmI GAATGC 6 cut(s) 158, 2956, 3165, 3503, 4050, 4164
BsnI GGCC 8 cut(s) 523, 641, 659, 1885, 3567, 3715, 4259, 4500
Bsp119I TTCGAA 1 cut(s) 1604
Bsp1286I GDGCHC 5 cut(s) 1298, 3578, 4014, 4126, 4435
Bsp1407I TGTACA 2 cut(s) 1792, 4423
Bsp19I CCATGG 4 cut(s) 673, 895, 3806, 4511
BspACI CCGC 3 cut(s) 3205, 3452, 3774
BspANI GGCC 8 cut(s) 523, 641, 659, 1885, 3567, 3715, 4259, 4500
BspDI ATCGAT 1 cut(s) 1769
BspHI TCATGA 1 cut(s) 4126
BspLI GGNNCC 8 cut(s) 259, 692, 1627, 1806, 2062, 2998, 3976, 4501
BspMAI CTGCAG 2 cut(s) 15, 4387
BspMI ACCTGC 2 cut(s) 169, 1333
BspQI GCTCTTC 3 cut(s) 694, 3583, 4440
BspT104I TTCGAA 1 cut(s) 1604
BspT107I GGYRCC 4 cut(s) 257, 1625, 2996, 3974
BspTI CTTAAG 2 cut(s) 1253, 3134
BsrDI GCAATG 3 cut(s) 446, 3106, 3666
BsrFI RCCGGY 1 cut(s) 3977
BsrGI TGTACA 2 cut(s) 1792, 4423
BssAI RCCGGY 1 cut(s) 3977
BssNI GRCGYC 1 cut(s) 4313
BssSI CACGAG 2 cut(s) 1220, 1968
BssT1I CCWWGG 9 cut(s) 261, 673, 895, 1142, 2716, 3368, 3806, 4302, 4511
Bst2BI CACGAG 2 cut(s) 1220, 1968
Bst6I CTCTTC 4 cut(s) 694, 2683, 3583, 4440
BstACI GRCGYC 1 cut(s) 4313
BstAFI CTTAAG 2 cut(s) 1253, 3134
BstAPI GCANNNNNTGC 1 cut(s) 4382
BstAUI TGTACA 2 cut(s) 1792, 4423
BstBAI YACGTR 1 cut(s) 2738
BstBI TTCGAA 1 cut(s) 1604
BstC8I GCNNGC 6 cut(s) 360, 1644, 2912, 3165, 3169, 3539
BstDSI CCRYGG 5 cut(s) 673, 895, 2148, 3806, 4511
BstENI CCTNNNNNAGG 1 cut(s) 2720
BstF5I GGATG 9 cut(s) 433, 1387, 1510, 1894, 2629, 2993, 3235, 3270, 3406
BstMAI GTCTC 4 cut(s) 1508, 2078, 2559, 4274
BstMCI CGRYCG 1 cut(s) 3209
BstNSI RCATGY 6 cut(s) 362, 1316, 1716, 2091, 2388, 4413
BstPAI GACNNNNGTC 2 cut(s) 897, 3738
BstSFI CTRYAG 7 cut(s) 11, 1809, 3736, 3824, 4015, 4383, 4392
BstV1I GCAGC 9 cut(s) 25, 385, 2104, 2407, 3773, 3995, 4127, 4375, 4397
BstV2I GAAGAC 8 cut(s) 177, 720, 2058, 2330, 3072, 3585, 3909, 3935
BstXI CCANNNNNNTGG 1 cut(s) 1207
Bsu15I ATCGAT 1 cut(s) 1769
Bsu36I CCTNAGG 1 cut(s) 2226
BsuI GTATCC 1 cut(s) 3319
BsuRI GGCC 8 cut(s) 523, 641, 659, 1885, 3567, 3715, 4259, 4500
BsuTUI ATCGAT 1 cut(s) 1769
BtgI CCRYGG 5 cut(s) 673, 895, 2148, 3806, 4511
BtgZI GCGATG 1 cut(s) 1755
BtrI CACGTC 3 cut(s) 1554, 2577, 3125
BtsCI GGATG 9 cut(s) 433, 1387, 1510, 1894, 2629, 2993, 3235, 3270, 3406
BtsIMutI CAGTG 7 cut(s) 536, 2254, 2793, 3081, 3539, 4216, 4279
BveI ACCTGC 2 cut(s) 169, 1333
Cac8I GCNNGC 6 cut(s) 360, 1644, 2912, 3165, 3169, 3539
CaiI CAGNNNCTG 2 cut(s) 4382, 4406
CciI TCATGA 1 cut(s) 4126
Cfr10I RCCGGY 1 cut(s) 3977
Cfr13I GGNCC 8 cut(s) 521, 691, 899, 1139, 2061, 2713, 3566, 4499
ClaI ATCGAT 1 cut(s) 1769
CseI GACGC 1 cut(s) 4321
Csp6I GTAC 9 cut(s) 258, 483, 1793, 2769, 3128, 4056, 4106, 4178, 4424
CspCI CAANNNNNGTGG 2 cut(s) 1840, 1875
CviQI GTAC 9 cut(s) 258, 483, 1793, 2769, 3128, 4056, 4106, 4178, 4424
DraIII CACNNNGTG 3 cut(s) 1652, 1970, 3260
DriI GACNNNNNGTC 2 cut(s) 2059, 2398
EaeI YGGCCR 1 cut(s) 1883
Eam1104I CTCTTC 4 cut(s) 694, 2683, 3583, 4440
Eam1105I GACNNNNNGTC 2 cut(s) 2059, 2398
EarI CTCTTC 4 cut(s) 694, 2683, 3583, 4440
Ecl136II GAGCTC 2 cut(s) 1296, 3576
Eco130I CCWWGG 9 cut(s) 261, 673, 895, 1142, 2716, 3368, 3806, 4302, 4511
Eco147I AGGCCT 2 cut(s) 659, 4259
Eco24I GRGCYC 3 cut(s) 1298, 3578, 4126
Eco32I GATATC 2 cut(s) 163, 2631
Eco47I GGWCC 5 cut(s) 691, 899, 1139, 2061, 2713
Eco53kI GAGCTC 2 cut(s) 1296, 3576
Eco57I CTGAAG 5 cut(s) 423, 444, 3564, 3579, 4429
Eco81I CCTNAGG 1 cut(s) 2226
EcoICRI GAGCTC 2 cut(s) 1296, 3576
EcoNI CCTNNNNNAGG 1 cut(s) 2720
EcoO109I RGGNCCY 3 cut(s) 691, 2713, 4499
EcoRI GAATTC 1 cut(s) 4248
EcoRV GATATC 2 cut(s) 163, 2631
EcoT14I CCWWGG 9 cut(s) 261, 673, 895, 1142, 2716, 3368, 3806, 4302, 4511
EcoT22I ATGCAT 2 cut(s) 1762, 2644
EcoT38I GRGCYC 3 cut(s) 1298, 3578, 4126
ErhI CCWWGG 9 cut(s) 261, 673, 895, 1142, 2716, 3368, 3806, 4302, 4511
FaqI GGGAC 7 cut(s) 704, 1505, 1564, 2350, 3726, 3788, 4364
FauI CCCGC 1 cut(s) 3767
FauNDI CATATG 1 cut(s) 195
FbaI TGATCA 3 cut(s) 1690, 1707, 3586
FblI GTMKAC 1 cut(s) 1321
Fnu4HI GCNGC 9 cut(s) 14, 399, 2118, 2421, 3762, 3984, 4116, 4386, 4389
FokI GGATG 9 cut(s) 440, 1394, 1517, 1901, 2636, 3000, 3242, 3277, 3393
FriOI GRGCYC 3 cut(s) 1298, 3578, 4126
Fsp4HI GCNGC 9 cut(s) 14, 399, 2118, 2421, 3762, 3984, 4116, 4386, 4389
GluI GCNGC 9 cut(s) 14, 399, 2118, 2421, 3762, 3984, 4116, 4386, 4389
GsaI CCCAGC 4 cut(s) 51, 3865, 4122, 4350
GsuI CTGGAG 3 cut(s) 2474, 3266, 4383
HaeIII GGCC 8 cut(s) 523, 641, 659, 1885, 3567, 3715, 4259, 4500
HapII CCGG 2 cut(s) 1886, 3978
HgaI GACGC 1 cut(s) 4321
Hin1I GRCGYC 1 cut(s) 4313
HindIII AAGCTT 4 cut(s) 817, 1583, 3071, 3314
HpaII CCGG 2 cut(s) 1886, 3978
HphI GGTGA 7 cut(s) 122, 761, 815, 852, 983, 1664, 3521
Hpy166II GTNNAC 7 cut(s) 568, 788, 1322, 1471, 1541, 3128, 3733
Hpy8I GTNNAC 7 cut(s) 568, 788, 1322, 1471, 1541, 3128, 3733
Hpy99I CGWCG 1 cut(s) 2886
HpyCH4IV ACGT 9 cut(s) 790, 949, 1553, 1735, 2576, 2737, 3124, 3282, 3835
HpySE526I ACGT 9 cut(s) 790, 949, 1553, 1735, 2576, 2737, 3124, 3282, 3835
Hsp92I GRCGYC 1 cut(s) 4313
KpnI GGTACC 1 cut(s) 261
Ksp22I TGATCA 3 cut(s) 1690, 1707, 3586
LguI GCTCTTC 3 cut(s) 694, 3583, 4440
LmnI GCTCC 7 cut(s) 1271, 1904, 2449, 3370, 4143, 4337, 4402
Lsp1109I GCAGC 9 cut(s) 25, 385, 2104, 2407, 3773, 3995, 4127, 4375, 4397
MaeII ACGT 9 cut(s) 790, 949, 1553, 1735, 2576, 2737, 3124, 3282, 3835
MfeI CAATTG 4 cut(s) 1854, 2463, 3117, 4059
MhlI GDGCHC 5 cut(s) 1298, 3578, 4014, 4126, 4435
MlyI GAGTC 7 cut(s) 1563, 2048, 2561, 2669, 2693, 3507, 4487
MmeI TCCRAC 2 cut(s) 2455, 2582
Mph1103I ATGCAT 2 cut(s) 1762, 2644
MroXI GAANNNNTTC 3 cut(s) 408, 703, 4078
MslI CAYNNNNRTG 4 cut(s) 96, 1109, 2165, 2375
MspA1I CMGCKG 4 cut(s) 380, 3205, 4118, 4388
MspCI CTTAAG 2 cut(s) 1253, 3134
MspI CCGG 2 cut(s) 1886, 3978
MunI CAATTG 4 cut(s) 1854, 2463, 3117, 4059
Mva1269I GAATGC 6 cut(s) 158, 2956, 3165, 3503, 4050, 4164
NcoI CCATGG 4 cut(s) 673, 895, 3806, 4511
NdeI CATATG 1 cut(s) 195
NlaIV GGNNCC 8 cut(s) 259, 692, 1627, 1806, 2062, 2998, 3976, 4501
NmeAIII GCCGAG 3 cut(s) 169, 182, 667
NmuCI GTSAC 5 cut(s) 466, 872, 943, 1132, 2685
NsiI ATGCAT 2 cut(s) 1762, 2644
NspI RCATGY 6 cut(s) 362, 1316, 1716, 2091, 2388, 4413
NspV TTCGAA 1 cut(s) 1604
PaeI GCATGC 1 cut(s) 362
PagI TCATGA 1 cut(s) 4126
PaqCI CACCTGC 1 cut(s) 169
PceI AGGCCT 2 cut(s) 659, 4259
PciI ACATGT 3 cut(s) 1312, 1712, 2087
PciSI GCTCTTC 3 cut(s) 694, 3583, 4440
PctI GAATGC 6 cut(s) 158, 2956, 3165, 3503, 4050, 4164
PdmI GAANNNNTTC 3 cut(s) 408, 703, 4078
PflMI CCANNNNNTGG 1 cut(s) 3296
PkrI GCNGC 9 cut(s) 15, 400, 2119, 2422, 3763, 3985, 4117, 4387, 4390
PleI GAGTC 7 cut(s) 1563, 2048, 2560, 2669, 2692, 3506, 4486
PpsI GAGTC 7 cut(s) 1563, 2048, 2560, 2669, 2692, 3506, 4486
Ppu21I YACGTR 1 cut(s) 2738
PpuMI RGGWCCY 2 cut(s) 691, 2713
PscI ACATGT 3 cut(s) 1312, 1712, 2087
PshAI GACNNNNGTC 2 cut(s) 897, 3738
Psp124BI GAGCTC 2 cut(s) 1298, 3578
Psp5II RGGWCCY 2 cut(s) 691, 2713
PspFI CCCAGC 4 cut(s) 47, 3861, 4118, 4346
PspN4I GGNNCC 8 cut(s) 259, 692, 1627, 1806, 2062, 2998, 3976, 4501
PspPI GGNCC 8 cut(s) 521, 691, 899, 1139, 2061, 2713, 3566, 4499
PspPPI RGGWCCY 2 cut(s) 691, 2713
PsrI GAACNNNNNNTAC 2 cut(s) 475, 507
PstI CTGCAG 2 cut(s) 15, 4387
PstNI CAGNNNCTG 2 cut(s) 4382, 4406
PvuII CAGCTG 3 cut(s) 380, 4118, 4388
RsaI GTAC 9 cut(s) 259, 484, 1794, 2770, 3129, 4057, 4107, 4179, 4425
RsaNI GTAC 9 cut(s) 258, 483, 1793, 2769, 3128, 4056, 4106, 4178, 4424
RseI CAYNNNNRTG 4 cut(s) 96, 1109, 2165, 2375
SacI GAGCTC 2 cut(s) 1298, 3578
SapI GCTCTTC 3 cut(s) 694, 3583, 4440
SatI GCNGC 9 cut(s) 14, 399, 2118, 2421, 3762, 3984, 4116, 4386, 4389
Sau96I GGNCC 8 cut(s) 521, 691, 899, 1139, 2061, 2713, 3566, 4499
SchI GAGTC 7 cut(s) 1563, 2048, 2561, 2669, 2693, 3507, 4487
SduI GDGCHC 5 cut(s) 1298, 3578, 4014, 4126, 4435
SfcI CTRYAG 7 cut(s) 11, 1809, 3736, 3824, 4015, 4383, 4392
SfuI TTCGAA 1 cut(s) 1604
SinI GGWCC 5 cut(s) 691, 899, 1139, 2061, 2713
SmiMI CAYNNNNRTG 4 cut(s) 96, 1109, 2165, 2375
SmlI CTYRAG 2 cut(s) 1253, 3134
SmoI CTYRAG 2 cut(s) 1253, 3134
SpeI ACTAGT 1 cut(s) 1679
SphI GCATGC 1 cut(s) 362
SseBI AGGCCT 2 cut(s) 659, 4259
SsiI CCGC 3 cut(s) 3205, 3452, 3774
SspI AATATT 6 cut(s) 586, 1491, 1609, 3799, 3933, 4151
SstI GAGCTC 2 cut(s) 1298, 3578
StuI AGGCCT 2 cut(s) 659, 4259
StyI CCWWGG 9 cut(s) 261, 673, 895, 1142, 2716, 3368, 3806, 4302, 4511
TaiI ACGT 9 cut(s) 793, 952, 1556, 1738, 2579, 2740, 3127, 3285, 3838
TatI WGTACW 5 cut(s) 482, 1792, 3127, 4105, 4423
TscAI CASTG 7 cut(s) 543, 2254, 2793, 3088, 3539, 4216, 4279
TseFI GTSAC 5 cut(s) 466, 872, 943, 1132, 2685
TseI GCWGC 9 cut(s) 13, 398, 2117, 2420, 3761, 3983, 4115, 4385, 4388
Tsp45I GTSAC 5 cut(s) 466, 872, 943, 1132, 2685
TspGWI ACGGA 3 cut(s) 787, 2198, 2992
TspRI CASTG 7 cut(s) 543, 2254, 2793, 3088, 3539, 4216, 4279
Van91I CCANNNNNTGG 1 cut(s) 3296
Vha464I CTTAAG 2 cut(s) 1253, 3134
VpaK11BI GGWCC 5 cut(s) 691, 899, 1139, 2061, 2713
XagI CCTNNNNNAGG 1 cut(s) 2720
XapI RAATTY 7 cut(s) 851, 1202, 2311, 2492, 3009, 3269, 4248
XbaI TCTAGA 1 cut(s) 3188
XceI RCATGY 6 cut(s) 362, 1316, 1716, 2091, 2388, 4413
XcmI CCANNNNNNNNNTGG 1 cut(s) 3958
XmiI GTMKAC 1 cut(s) 1321
XmnI GAANNNNTTC 3 cut(s) 408, 703, 4078
Zsp2I ATGCAT 2 cut(s) 1762, 2644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.