Prupe.5G145400_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000526
Physical Location & Seq
Forward (+)
13368420 .. 13375260
6841 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G145400.1

Sequence Viewer

Length: 5169 bp
ATGGCGACACCGAGAGAGCACATAGAAGAAATACGCATGAAAAAGTTCTCCATTGGAGGGGAACTCAACCCTTTATCTGAAGATCTTCACCACGCTGTCGAGCATCTCTCTGCTGAGCTCTATTCTAAAGATGTCCACTTTCTCATGGAACTCATCCAGAATGCAGAAGATAATCAGTACTCAGAGGGGGTTGATCCATCACTTGAGTTTGTCTTAACGTCCCGGGATATAACCGGCACAGGGGCTCCTGCCACATTGCTTGTTTTCAATAATGAGAAGGGATTTTCTCCTACAAACATAGAGTCTATTTGCGGTATTGGACGGTCGACAAAGAAAGGCAACCGGAATCAGGGCTATATTGGGGAGAAAGGAATTGGTTTCAAGAGCGTGTTTCTGATCACAGCTCAGCCTTACATTTTTAGTAATGGTTATCAAATAAGGTTCAATGAAGAGCCTTGTATGCATTGCGATCTTGGGTACATAGTTCCAGAATGGGTTGAGGAAAGCCCGACTCTATCTGACATAACAGAGATATATGGTTCTGGTTCTGCCCTTCCAACCACAACACTGATCTTGCCTCTAAAGCCTGACAAGGTCAAACCTGTGAAGCAGCAGCTCTCAAGCATGCATCCTGAAGTTCTCTTGTTTCTTGCAAAGATTAAGCGGCTCTCGGTTAGGGAAGATAATGAGGATCCAAGGCTCAACACTGTAACCGCGATATCAATATCAAGTGAGACTGATTTTGTGACGCGGAAGAACATTGATGCTGATTCCTACACGCTCCATCTCTCTGCAGAAGAAAATGGTAATGAGTTGGAAACTGAATGCAGCTATTATATGTGGAAGCAGAAGTTTCCTGTCAAGCAAGAATGCAGAGATGAGAAGAGAATGGAGGTAGATGAGTGGGTGATCACGCTAGCATTTCCATATGGAGAACGCCTTAATAGAGGAACAAGCTCACCCGGGGTCTATGCATTCCTTCCCACTGAAATGTTTACAAATTTGCCCTTCATAATTCAGGCAGATTTTCTTTTAGCATCATCAAGAGAAAATATTCTATTGGACAAGAAGTGGAATCAAGGAATTCTGAACTGTGTGCCCTCTGCATTTATTAATGCATTTCTCTCTCTCGTGAAAACTATAGAAGATGCACCAGTATCTAGTTTGCCTCCTTTTTTCAGGTTCTTGCCTGTCCAGGACTCTAGATATGATGAATTGAATGTTGTGAGGGAGTCAATCAAAGCACAGCTGGTTGAGAAAGACATTGTACCATGTGAGCCGCACAAGGAGCAGAAGTTCTTCCACAAACCTTGTGAAGTGGGTAGGTTATTGCCTGATTTTTGGAATATTTTGATAGAGGCCAGGGAAGTAGGGGTAAGCTTGCCGAATCTCTCTTCCCATGGAAAATATGTTTTGTGTCATTCATTTGACCAAGAGGAGTATGATCACATTCTGAGTTTCCTTGGAGTTGAACCAGTGGATGATGAATGGTATGCTAAATGCATTCAGAGTTCCAATCTGGTAGTTGGAGTGTCAGAAGATGTCTACTTGGAACTTCTACTGTTTATTGCTGATAATTGGTGGCCCAAATTTCTCTGCACCAACATCAAGAACATACCACTGATAAAATATGTGGATCTTGATGAGGATGTATCTTTGTGCAGCTTGAGCTCAATGCAGACTGGTAAAAAGAACGTTTGCCTATCCCGTCATTCTTGTCATGTCTCATGGCTGATTGACTGGAACAGGGAATTTATATCTGCGGCAAGTCTTCTTTTTATGCCGAAAAGAACCCAGGAAGCTATCCAGTTATGTTCTAACAAGGACAAGTTGGTGAAATGGCTTGCGGAAGAAATGAAGGTTGCAGCTGTAAATGTACATGAATATGCAGTTTGCCTCTATAAGTCTAATGGTATTGAACGGAAACCTGCTATTGCATATGCCCACTTTCTGTATCATTCATTCTGCAAGAGCTATATCTCTGATCTTGAAATTGTTGATTTATGTGGTAAGATGCCACTCGTGGATAACTACGGGGACGTCATCAGACAAAGGAGAGGAGTTATTGTCCCTGCCAACGAAAGCAAATGGGCGGGGTTTACTGATTCGAATCTGTGGAAAGAAGACGGCTTTGTTCAGTTAGGAGAAGATTACATGAATCCAGGCCGCTTTGCAGGTCAAATTACAGAACAGAAGCAGCTCCTTGAGTTCCTCAAAGATCATGCTGGAGCTTCGGATGTCCCTTATATTTCTGCTCCCAATGCCTGTCTTCCAGCTCTATCTGCAACACTTAGTATAGAAAAGGTATTCTTGCTTTTGGACTGGATTCGCCATCTGAGATATCAAAGGGTTCACATCCCGGAGAAGTTCTTGAAATGTATAAAGGAAGGTAGTTGGTTGAAAGTTACTTTGAATGGATTTTCTGTGTGCAGGCCACCTTCGGAGTCATTTGTACTTACCCCATCACGGGGAAACATTTTGCAGAATGGATCCGTTTTTGTTGACATCCCATTGGTTGATAAGAATTATTATGGTGAGAGAATTGATGGTTACAAGGAGGAGCTGAAAACAATTGGAGTGATGTTTGAATTTGGAGAAGCATGTGAGTTTATTGGGAAGCATCTTATGTCTCTGGCAGCTTCGTCCACTTTACCTAGAGGGAACGTACTTTCGATACTACATTTTATCAAGCTTTTGAGGGACAAATGTCTTCCCCCAGACGATTTCATCCGCAGTATCAGAAAAGGACAATGGCTTAAAACAGAATCTCATGGTTACAGGTCTCCAGATGGATCTGTCTTGTTTGACCAAGAGTGGATACTTGCATCCAAAATTAGTGACATTCCCTTCATTGATCGGGAGGTATACGGTGAAGAAATCCTTGATTTCAAAACAGAACTTGAGTTGCTTGGTGTGGTTGTCTCGTTCAACAAAAATTACCAGCTTGTTGCCGACCACCTAAAGTCACCGTCATGCTTAACTTCTCTAGCACCTGAGGCTGTTCTACTAATGCTGCAAATTATGCACATTTCAAATTCATCCAATAAGATTGTCGAAGCACTGAGAGGAAAAAAATGCCTGAAGACAAATAATGGTTACAAGTCTCCAAGTGAATGTCTCTTGTTTCACCCTGAATGGGGTTGCCTTCTTCAGGTCTTTAGTGGTGTCCCATTAATTGATCATAATTTGTATGGAGACATAATCTTTTCCTTCAGAGATGAGTTGAGAAAGATAGGGGTGGTCGTTGATTTTGAAGAGGCTGCAAAAGTATTTGCTCACCATTTCCGGCAGGCTTCCATCACCAAAGAAAATGTAGAAGCATTTCTGTCCTGTTACAGAAAGCTGGAGGGAACTCCTTTTAAATTTCCTGCAGATCTTAAGAGCTGCATTTGCAAAGAAAAGTGGCTGCGTACTCGCCTTGGTGATTATAGATCTCCAAGAGAGTGCATTCTGTTTTGTTCTGATTGGGAATCTCTCTCTCCCATTTGCCGTCTCCCTTTTATTGACGATTCTGACACTTGTTATGGCAAGAACATTCATGAATATAAGCAAGAGCTGAAGAGCTTGGGGGTCGTTGTGGAATTCAAGGACGGGGTCAAGTTTGTGCCTTCTTGTCTATATCTTCCCCAGAATCCGAGGAGCATTTCTCGAGAAAATGCACTTGCTTTGCTGGACTGCATACATATTTTATTGGAGGAGAAAGATTACTCCTTTCCTGATGTGTTCACGAAGAAAGTTTCTCAGCCATGGCTGAAGGCTCATGATGGTTACGAGCCTCCAAGCAAGTGCTTGTTGTTTGATTCTGAGTTTGATAAGTATCTAAAGCAGACTGATGGGCCTTTCATTGATGAAGAGTTTTATGGTTCTAAAATTACAACATACAGAAAAGAGCTCTCTGAAATAGGGGTGATTGTTGAAGTGGACAAAGGATGTCCTTTGCTTGCCAGCCAGCTTGCTCTTCATGATGAATTGTCCACTTTTGTTCGAGTATACAGTTACTTGAGTGAGTTTAAATGGGAGCCAAATAGCAAGGCTGACAAAAGGATCTGGATTCAAAAGGGAAATCAGAATGGACAGTGGGTTAATCCAGAAGAGTGTGTTTTGTATGACAAGGATGAGCTGTTTGGCTTGCAGTTGACTGTTCTGGAGAATTACTTTGATCATAACCTACTCGGGTTCTTCTCCAGTGCTTACAAGGTAAAGCCCCGTCCATCAATTGATGACTACTGCAAGCTCTGGAAGGTGTGGGAAAGTTCTGAAACTGGATTGTCTCATGATCAATGTTGCAAGTTCTGGGGGTATGTTTCAAAGAGCCGGAGTTTAAAAACAGAGAAAGCTCTTCCTGAGGCCTCGGTCAAAGTACCTGAAAATAAATCAAAAACAGAGAAAGCTCTTTCTGAGGCCTTGGTGAAAGTACCTGTTACTTCAGGCTCAGATGAAATCTTGTTGTTAAACAAGTGCGACGTTTTCCTTCCTGATGACCTTCAACTTAAGGATCTTTTTGAAAAATCTTCTACTCACCCCTTATTTGTTTGGTATCCTCAGCCAAGCTTGCCTGACTTGCCACGGACTACATTGCTTGAAATGTATAGGAAAATTGGTGTTCGAGCTATTTCTGAATCTGTACAGAAGGAAGAACTGTCCGTAGAAAATGGTGTTGATGAACAGGTAATTCCAACGGAGAAATTGATCGGGAAAGAGCTGCTCAAGCTGATTCTTGGTTTTCTAGCTTGTCCTCCCAATGAAATGGAAGCAGGGAAGAGGCAAAAAGCTGTCCAAGGTCTTCTCAATCTCGCTGTCGTAGAGACAACCGAACCGATCACTGTAAACTATAACTTACCATTGTCTTCTGGGGAGACTTTGAATGTGAGAGCAAGCCGCAAGATAAGATGGGACAGAGAGATGTCCAAATTTTTCACTCAGAAAATTGACAGGTCTGGAGGACATAAAAGTATCATTGAGTTTGCCACATATTTCTCTCAAGTAATATCTGAAGGTGTCTTGTGGGAGCACACTGATCATATCCCTGCACTTTCTGAGCTGATCAAGTTGGCCTTTGTGCTAGAATTTAATGAGGAAGCAGTTGATTTTTTGATGAAGTCCAAGAATTTGCAAATCTTCATTGAGGATGAAGAGTTTCTCAATTCTACCTTCCCTTCTCCCACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1723

Amino Acids

196.28

Weight (kDa)

5.43

Isoelectric Point (pI)

47.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28020 AT3G48770 AT3G48770
fragaria_vesca FvH4_5g09020 FvH4_5g09020
malus_domestica MD06G1092400.v1.1 MD06G1134600.v1.1 MD06G1134700.v1.1 MD06G1134800.v1.1 MD06G1135200.v1.1 MD06G1135400.v1.1 MD06G1135500.v1.1 MD08G1137600.v1.1 MD15G1395100.v1.1
prunus_persica Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145600_v2.0.a1 Prupe.5G145800_v2.0.a1 Prupe.5G145900_v2.0.a1
pyrus_communis pycom06g12550 pycom06g12570 pycom06g12580 pycom06g12590 pycom06g12610 pycom06g12630 pycom06g12640 pycom06g12650 pycom08g06280
rosa_chinensis RchiOBHm_Chr6g0249321 RchiOBHm_Chr6g0275111 RchiOBHm_Chr7g0189191 RchiOBHm_Chr7g0189261
rosa_laevigata RLG00000004622 RLG00000004623 RLG00000008237 RLG00000008238 RLG00000023303 RLG00000035187
rosa_multiflora Rmu_co8294311.1_g000001 Rmu_co8389833.1_g000001 Rmu_sc0001044.1_g000004 Rmu_sc0001329.1_g000011 Rmu_sc0006012.1_g000010 Rmu_sc0007286.1_g000032 Rmu_sc0009360.1_g000016 Rmu_sc0011817.1_g000001 Rmu_sc0015050.1_g000001
rosa_roxburghii Rroxscaffold_1G00064000 Rroxscaffold_3G00265460 Rroxscaffold_3G00265580 Rroxscaffold_3G00265640 Rroxscaffold_3G00265690 Rroxscaffold_3G00265700 Rroxscaffold_7G00193620 Rroxscaffold_7G00204550 Rroxscaffold_7G00217350
rosa_rugosa Rorug01G0465500 Rorug06G0088500 Rorug06G0496700 Rorug06G0496700
rosa_samantha Rh1AG064800 Rh2BG015100 Rh2BG493500 Rh2DG017300 Rh2DG502500 Rh4BG208500 Rh5BG528500 Rh5CG552900 Rh6CG207800 Rh6DG197900 Rh6DG198000 Rh7AG101300 Rh7AG101400 Rh7AG101600 Rh7BG104100 Rh7BG104200 Rh7CG105600 Rh7CG105700 Rh7DG103200 Rh7DG103400
rosa_wichuraiana Rw1G007300 Rw5G034670 Rw7G008750 Rw7G008780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2043
Acc36I ACCTGC 2 cut(s) 1936, 2165
AccI GTMKAC 4 cut(s) 326, 1545, 2865, 3990
AccII CGCG 2 cut(s) 716, 751
AclI AACGTT 1 cut(s) 1695
AclWI GGATC 9 cut(s) 188, 686, 699, 1644, 2484, 2497, 2800, 4052, 4503
AcuI CTGAAG 9 cut(s) 99, 654, 3101, 3134, 3196, 3578, 3773, 4410, 5013
AcyI GRCGYC 1 cut(s) 2040
AfiI CCNNNNNNNGG 9 cut(s) 57, 240, 349, 2466, 2467, 2468, 3136, 3137, 3151
AflII CTTAAG 2 cut(s) 3377, 4490
AjnI CCWGG 4 cut(s) 1194, 1361, 1794, 2161
AloI GAACNNNNNNTCC 2 cut(s) 1280, 1312
Alw21I GWGCWC 5 cut(s) 21, 120, 1673, 3894, 5013
AlwI GGATC 9 cut(s) 188, 686, 699, 1644, 2484, 2497, 2800, 4052, 4503
Ama87I CYCGRG 4 cut(s) 222, 962, 3648, 4172
AoxI GGCC 8 cut(s) 1359, 1583, 2164, 2432, 3836, 4347, 4401, 5052
ArsI GACNNNNNNTTYG 2 cut(s) 287, 319
AseI ATTAAT 2 cut(s) 1113, 3173
Asp700I GAANNNNTTC 6 cut(s) 44, 84, 1053, 1298, 3321, 5136
AspS9I GGNCC 2 cut(s) 1584, 3836
AsuC2I CCSGG 5 cut(s) 223, 224, 963, 964, 2360
AsuII TTCGAA 1 cut(s) 2108
AsuNHI GCTAGC 1 cut(s) 916
AvaI CYCGRG 4 cut(s) 222, 962, 3648, 4172
AxyI CCTNAGG 2 cut(s) 2994, 4344
BaeGI GKGCMC 1 cut(s) 1101
BamHI GGATCC 2 cut(s) 691, 2489
BanII GRGCYC 4 cut(s) 120, 247, 1673, 3894
BauI CACGAG 2 cut(s) 1130, 2021
BbsI GAAGAC 7 cut(s) 1763, 2130, 2261, 2702, 3089, 4775, 4839
Bbv12I GWGCWC 5 cut(s) 21, 120, 1673, 3894, 5013
BbvCI CCTCAGC 1 cut(s) 4542
BceAI ACGGC 2 cut(s) 2143, 3474
BciT130I CCWGG 4 cut(s) 1196, 1363, 1796, 2163
BciVI GTATCC 2 cut(s) 2811, 4548
BclI TGATCA 8 cut(s) 396, 909, 1444, 3178, 4159, 4276, 5017, 5043
BcnI CCSGG 5 cut(s) 223, 224, 963, 964, 2360
BfaI CTAG 8 cut(s) 917, 1161, 1203, 2655, 2987, 4727, 5063, 5167
BfmI CTRYAG 3 cut(s) 792, 1140, 3369
BfrI CTTAAG 2 cut(s) 3377, 4490
BfuAI ACCTGC 2 cut(s) 1936, 2165
BfuI GTATCC 2 cut(s) 2811, 4548
BglII AGATCT 3 cut(s) 82, 3373, 3431
BlpI GCTNAGC 2 cut(s) 114, 405
BmcAI AGTACT 1 cut(s) 179
Bme1390I CCNGG 9 cut(s) 223, 224, 963, 964, 1196, 1363, 1796, 2163, 2360
BmeT110I CYCGRG 4 cut(s) 222, 962, 3648, 4172
BmgT120I GGNCC 2 cut(s) 1584, 3836
BmiI GGNNCC 4 cut(s) 246, 693, 2491, 4020
BmrFI CCNGG 9 cut(s) 223, 224, 963, 964, 1196, 1363, 1796, 2163, 2360
BmsI GCATC 8 cut(s) 112, 637, 754, 1046, 1138, 2004, 2629, 2834
BmtI GCTAGC 1 cut(s) 920
BoxI GACNNNNGTC 1 cut(s) 2706
BpiI GAAGAC 7 cut(s) 1763, 2130, 2261, 2702, 3089, 4775, 4839
BplI GAGNNNNNCTC 6 cut(s) 48, 80, 92, 124, 3631, 3663
BpmI CTGGAG 6 cut(s) 2247, 2769, 3365, 4166, 4168, 4959
Bpu10I CCTNAGC 1 cut(s) 4542
Bpu1102I GCTNAGC 2 cut(s) 114, 405
Bpu14I TTCGAA 1 cut(s) 2108
BpuEI CTTGAG 8 cut(s) 224, 604, 1687, 2225, 2921, 4021, 4691, 4965
BpuMI CCSGG 5 cut(s) 223, 224, 963, 964, 2360
BsaBI GATNNNNATC 2 cut(s) 2109, 3816
BsaHI GRCGYC 1 cut(s) 2040
BsaI GGTCTC 1 cut(s) 2787
BsaWI WCCGGW 1 cut(s) 342
BsaXI ACNNNNNCTCC 6 cut(s) 229, 259, 1280, 1310, 2052, 2082
Bsc4I CCNNNNNNNGG 9 cut(s) 57, 240, 349, 2466, 2467, 2468, 3136, 3137, 3151
Bse118I RCCGGY 1 cut(s) 233
Bse1I ACTGG 8 cut(s) 1154, 1475, 1687, 1745, 1807, 2327, 4185, 4267
Bse21I CCTNAGG 2 cut(s) 2994, 4344
Bse3DI GCAATG 3 cut(s) 254, 463, 4574
Bse8I GATNNNNATC 2 cut(s) 2109, 3816
BseBI CCWGG 4 cut(s) 1196, 1363, 1796, 2163
BseJI GATNNNNATC 2 cut(s) 2109, 3816
BseLI CCNNNNNNNGG 9 cut(s) 57, 240, 349, 2466, 2467, 2468, 3136, 3137, 3151
BseMI GCAATG 3 cut(s) 254, 463, 4574
BseNI ACTGG 8 cut(s) 1154, 1475, 1687, 1745, 1807, 2327, 4185, 4267
BseRI GAGGAG 5 cut(s) 1451, 2073, 2573, 3652, 3710
BseSI GKGCMC 1 cut(s) 1101
BsgI GTGCAG 4 cut(s) 1582, 1681, 2449, 5013
Bsh1236I CGCG 2 cut(s) 716, 751
Bsh1285I CGRYCG 1 cut(s) 326
BshFI GGCC 8 cut(s) 1361, 1585, 2166, 2434, 3838, 4349, 4403, 5054
BsiEI CGRYCG 1 cut(s) 326
BsiHKAI GWGCWC 5 cut(s) 21, 120, 1673, 3894, 5013
BsiHKCI CYCGRG 4 cut(s) 222, 962, 3648, 4172
BsiSI CCGG 7 cut(s) 223, 234, 343, 963, 2360, 3286, 4315
BslFI GGGAC 7 cut(s) 205, 2051, 2054, 2225, 2714, 3152, 4907
BslI CCNNNNNNNGG 9 cut(s) 57, 240, 349, 2466, 2467, 2468, 3136, 3137, 3151
BsmBI CGTCTC 1 cut(s) 3497
BsmFI GGGAC 7 cut(s) 205, 2051, 2054, 2225, 2714, 3152, 4907
BsmI GAATGC 6 cut(s) 166, 830, 875, 974, 1503, 3447
BsnI GGCC 8 cut(s) 1361, 1585, 2166, 2434, 3838, 4349, 4403, 5054
Bso31I GGTCTC 1 cut(s) 2787
BsoBI CYCGRG 4 cut(s) 222, 962, 3648, 4172
Bsp119I TTCGAA 1 cut(s) 2108
Bsp1286I GDGCHC 7 cut(s) 21, 120, 247, 1101, 1673, 3894, 5013
Bsp1407I TGTACA 2 cut(s) 1876, 4624
Bsp1720I GCTNAGC 2 cut(s) 114, 405
Bsp19I CCATGG 2 cut(s) 1399, 3746
BspANI GGCC 8 cut(s) 1361, 1585, 2166, 2434, 3838, 4349, 4403, 5054
BspFNI CGCG 2 cut(s) 716, 751
BspHI TCATGA 4 cut(s) 3538, 3760, 3961, 4273
BspLI GGNNCC 4 cut(s) 246, 693, 2491, 4020
BspMAI CTGCAG 2 cut(s) 796, 3373
BspMI ACCTGC 2 cut(s) 1936, 2165
BspOI GCTAGC 1 cut(s) 920
BspPI GGATC 9 cut(s) 188, 686, 699, 1644, 2484, 2497, 2800, 4052, 4503
BspQI GCTCTTC 4 cut(s) 444, 3554, 3963, 4344
BspT104I TTCGAA 1 cut(s) 2108
BspTI CTTAAG 2 cut(s) 3377, 4490
BspTNI GGTCTC 1 cut(s) 2787
BsrDI GCAATG 3 cut(s) 254, 463, 4574
BsrFI RCCGGY 1 cut(s) 233
BsrGI TGTACA 2 cut(s) 1876, 4624
BsrI ACTGG 8 cut(s) 1154, 1475, 1687, 1745, 1807, 2327, 4185, 4267
BssAI RCCGGY 1 cut(s) 233
BssNAI GTATAC 2 cut(s) 2866, 3991
BssNI GRCGYC 1 cut(s) 2040
BssSI CACGAG 2 cut(s) 1130, 2021
BssT1I CCWWGG 7 cut(s) 695, 1399, 1462, 3418, 3746, 4404, 4777
Bst1107I GTATAC 2 cut(s) 2866, 3991
Bst2BI CACGAG 2 cut(s) 1130, 2021
Bst2UI CCWGG 4 cut(s) 1196, 1363, 1796, 2163
BstACI GRCGYC 1 cut(s) 2040
BstAFI CTTAAG 2 cut(s) 3377, 4490
BstAPI GCANNNNNTGC 1 cut(s) 3022
BstAUI TGTACA 2 cut(s) 1876, 4624
BstBI TTCGAA 1 cut(s) 2108
BstDSI CCRYGG 3 cut(s) 1399, 3746, 4565
BstENI CCTNNNNNAGG 1 cut(s) 3149
BstFNI CGCG 2 cut(s) 716, 751
BstMCI CGRYCG 1 cut(s) 326
BstNI CCWGG 4 cut(s) 1196, 1363, 1796, 2163
BstNSI RCATGY 2 cut(s) 628, 2604
BstPAI GACNNNNGTC 1 cut(s) 2706
BstSCI CCNGG 9 cut(s) 221, 222, 961, 962, 1194, 1361, 1794, 2161, 2358
BstSFI CTRYAG 3 cut(s) 792, 1140, 3369
BstSLI GKGCMC 1 cut(s) 1101
BstUI CGCG 2 cut(s) 716, 751
BstV2I GAAGAC 7 cut(s) 1763, 2130, 2261, 2702, 3089, 4775, 4839
BstX2I RGATCY 9 cut(s) 82, 691, 1636, 2489, 2792, 3373, 3431, 4044, 4495
BstXI CCANNNNNNTGG 1 cut(s) 4747
BstYI RGATCY 9 cut(s) 82, 691, 1636, 2489, 2792, 3373, 3431, 4044, 4495
BstZ17I GTATAC 2 cut(s) 2866, 3991
Bsu36I CCTNAGG 2 cut(s) 2994, 4344
BsuI GTATCC 2 cut(s) 2811, 4548
BsuRI GGCC 8 cut(s) 1361, 1585, 2166, 2434, 3838, 4349, 4403, 5054
BtgI CCRYGG 3 cut(s) 1399, 3746, 4565
BveI ACCTGC 2 cut(s) 1936, 2165
CciI TCATGA 4 cut(s) 3538, 3760, 3961, 4273
Cfr10I RCCGGY 1 cut(s) 233
Cfr13I GGNCC 2 cut(s) 1584, 3836
Cfr9I CCCGGG 2 cut(s) 222, 962
CseI GACGC 1 cut(s) 757
CspCI CAANNNNNGTGG 4 cut(s) 241, 276, 1292, 1327
DraI TTTAAA 3 cut(s) 3361, 4012, 4323
Ecl136II GAGCTC 3 cut(s) 118, 1671, 3892
Eco130I CCWWGG 7 cut(s) 695, 1399, 1462, 3418, 3746, 4404, 4777
Eco147I AGGCCT 2 cut(s) 4349, 4403
Eco24I GRGCYC 4 cut(s) 120, 247, 1673, 3894
Eco31I GGTCTC 1 cut(s) 2787
Eco32I GATATC 2 cut(s) 720, 2342
Eco53kI GAGCTC 3 cut(s) 118, 1671, 3892
Eco57I CTGAAG 9 cut(s) 99, 654, 3101, 3134, 3196, 3578, 3773, 4410, 5013
Eco81I CCTNAGG 2 cut(s) 2994, 4344
Eco88I CYCGRG 4 cut(s) 222, 962, 3648, 4172
EcoICRI GAGCTC 3 cut(s) 118, 1671, 3892
EcoNI CCTNNNNNAGG 1 cut(s) 3149
EcoRI GAATTC 2 cut(s) 1083, 3581
EcoRII CCWGG 4 cut(s) 1194, 1361, 1794, 2161
EcoRV GATATC 2 cut(s) 720, 2342
EcoT14I CCWWGG 7 cut(s) 695, 1399, 1462, 3418, 3746, 4404, 4777
EcoT22I ATGCAT 5 cut(s) 465, 630, 976, 1120, 1505
EcoT38I GRGCYC 4 cut(s) 120, 247, 1673, 3894
ErhI CCWWGG 7 cut(s) 695, 1399, 1462, 3418, 3746, 4404, 4777
Esp3I CGTCTC 1 cut(s) 3497
FalI AAGNNNNNCTT 6 cut(s) 2294, 2326, 2865, 2897, 5039, 5071
FaqI GGGAC 7 cut(s) 205, 2051, 2054, 2225, 2714, 3152, 4907
FauI CCCGC 1 cut(s) 2086
FauNDI CATATG 2 cut(s) 928, 1939
FbaI TGATCA 8 cut(s) 396, 909, 1444, 3178, 4159, 4276, 5017, 5043
FblI GTMKAC 4 cut(s) 326, 1545, 2865, 3990
FriOI GRGCYC 4 cut(s) 120, 247, 1673, 3894
FspBI CTAG 8 cut(s) 917, 1161, 1203, 2655, 2987, 4727, 5063, 5167
GsuI CTGGAG 6 cut(s) 2247, 2769, 3365, 4166, 4168, 4959
HaeIII GGCC 8 cut(s) 1361, 1585, 2166, 2434, 3838, 4349, 4403, 5054
HapII CCGG 7 cut(s) 223, 234, 343, 963, 2360, 3286, 4315
HgaI GACGC 1 cut(s) 757
Hin1I GRCGYC 1 cut(s) 2040
HincII GTYRAC 3 cut(s) 327, 2503, 4137
HindII GTYRAC 3 cut(s) 327, 2503, 4137
HindIII AAGCTT 3 cut(s) 1378, 2690, 4549
HpaII CCGG 7 cut(s) 223, 234, 343, 963, 2360, 3286, 4315
Hpy99I CGWCG 1 cut(s) 4466
HpyCH4IV ACGT 5 cut(s) 218, 1695, 2040, 2664, 4464
HpySE526I ACGT 5 cut(s) 218, 1695, 2040, 2664, 4464
Hsp92I GRCGYC 1 cut(s) 2040
Ksp22I TGATCA 8 cut(s) 396, 909, 1444, 3178, 4159, 4276, 5017, 5043
LguI GCTCTTC 4 cut(s) 444, 3554, 3963, 4344
LweI GCATC 8 cut(s) 112, 637, 754, 1046, 1138, 2004, 2629, 2834
MaeI CTAG 8 cut(s) 917, 1161, 1203, 2655, 2987, 4727, 5063, 5167
MaeII ACGT 5 cut(s) 218, 1695, 2040, 2664, 4464
MfeI CAATTG 2 cut(s) 2571, 4215
MflI RGATCY 9 cut(s) 82, 691, 1636, 2489, 2792, 3373, 3431, 4044, 4495
MhlI GDGCHC 7 cut(s) 21, 120, 247, 1101, 1673, 3894, 5013
MlyI GAGTC 5 cut(s) 311, 505, 1193, 1241, 2453
MmeI TCCRAC 4 cut(s) 581, 795, 1507, 4700
Mph1103I ATGCAT 5 cut(s) 465, 630, 976, 1120, 1505
MroXI GAANNNNTTC 6 cut(s) 44, 84, 1053, 1298, 3321, 5136
MslI CAYNNNNRTG 3 cut(s) 989, 1884, 2971
MspA1I CMGCKG 2 cut(s) 1249, 1868
MspCI CTTAAG 2 cut(s) 3377, 4490
MspI CCGG 7 cut(s) 223, 234, 343, 963, 2360, 3286, 4315
MspR9I CCNGG 9 cut(s) 223, 224, 963, 964, 1196, 1363, 1796, 2163, 2360
MunI CAATTG 2 cut(s) 2571, 4215
Mva1269I GAATGC 6 cut(s) 166, 830, 875, 974, 1503, 3447
MvaI CCWGG 4 cut(s) 1196, 1363, 1796, 2163
MvnI CGCG 2 cut(s) 716, 751
NciI CCSGG 5 cut(s) 223, 224, 963, 964, 2360
NcoI CCATGG 2 cut(s) 1399, 3746
NdeI CATATG 2 cut(s) 928, 1939
NheI GCTAGC 1 cut(s) 916
NlaIV GGNNCC 4 cut(s) 246, 693, 2491, 4020
NmuCI GTSAC 3 cut(s) 745, 2837, 2964
NsiI ATGCAT 5 cut(s) 465, 630, 976, 1120, 1505
NspI RCATGY 2 cut(s) 628, 2604
NspV TTCGAA 1 cut(s) 2108
PaeI GCATGC 1 cut(s) 628
PaeR7I CTCGAG 1 cut(s) 3648
PagI TCATGA 4 cut(s) 3538, 3760, 3961, 4273
PceI AGGCCT 2 cut(s) 4349, 4403
PciSI GCTCTTC 4 cut(s) 444, 3554, 3963, 4344
PctI GAATGC 6 cut(s) 166, 830, 875, 974, 1503, 3447
PdmI GAANNNNTTC 6 cut(s) 44, 84, 1053, 1298, 3321, 5136
PflFI GACNNNGTC 2 cut(s) 593, 3593
PfoI TCCNGGA 2 cut(s) 1194, 2358
PleI GAGTC 5 cut(s) 310, 505, 1193, 1240, 2452
PpsI GAGTC 5 cut(s) 310, 505, 1193, 1240, 2452
PshAI GACNNNNGTC 1 cut(s) 2706
PshBI ATTAAT 2 cut(s) 1113, 3173
Psp124BI GAGCTC 3 cut(s) 120, 1673, 3894
Psp1406I AACGTT 1 cut(s) 1695
Psp6I CCWGG 4 cut(s) 1194, 1361, 1794, 2161
PspGI CCWGG 4 cut(s) 1194, 1361, 1794, 2161
PspN4I GGNNCC 4 cut(s) 246, 693, 2491, 4020
PspPI GGNCC 2 cut(s) 1584, 3836
PstI CTGCAG 2 cut(s) 796, 3373
PsuI RGATCY 9 cut(s) 82, 691, 1636, 2489, 2792, 3373, 3431, 4044, 4495
PsyI GACNNNGTC 2 cut(s) 593, 3593
PvuII CAGCTG 2 cut(s) 1249, 1868
RseI CAYNNNNRTG 3 cut(s) 989, 1884, 2971
SacI GAGCTC 3 cut(s) 120, 1673, 3894
SalI GTCGAC 1 cut(s) 325
SapI GCTCTTC 4 cut(s) 444, 3554, 3963, 4344
Sau96I GGNCC 2 cut(s) 1584, 3836
ScaI AGTACT 1 cut(s) 179
SchI GAGTC 5 cut(s) 311, 505, 1193, 1241, 2453
ScrFI CCNGG 9 cut(s) 223, 224, 963, 964, 1196, 1363, 1796, 2163, 2360
SduI GDGCHC 7 cut(s) 21, 120, 247, 1101, 1673, 3894, 5013
SfaNI GCATC 8 cut(s) 112, 637, 754, 1046, 1138, 2004, 2629, 2834
SfcI CTRYAG 3 cut(s) 792, 1140, 3369
Sfr274I CTCGAG 1 cut(s) 3648
SfuI TTCGAA 1 cut(s) 2108
SlaI CTCGAG 1 cut(s) 3648
SmaI CCCGGG 2 cut(s) 224, 964
SmiMI CAYNNNNRTG 3 cut(s) 989, 1884, 2971
SphI GCATGC 1 cut(s) 628
SseBI AGGCCT 2 cut(s) 4349, 4403
SspI AATATT 2 cut(s) 1054, 1348
SspMI CTAG 8 cut(s) 917, 1161, 1203, 2655, 2987, 4727, 5063, 5167
SstI GAGCTC 3 cut(s) 120, 1673, 3894
StuI AGGCCT 2 cut(s) 4349, 4403
StyD4I CCNGG 9 cut(s) 221, 222, 961, 962, 1194, 1361, 1794, 2161, 2358
StyI CCWWGG 7 cut(s) 695, 1399, 1462, 3418, 3746, 4404, 4777
TaiI ACGT 5 cut(s) 221, 1698, 2043, 2667, 4467
TaqI TCGA 8 cut(s) 99, 326, 2108, 2672, 3054, 3649, 3985, 4606
TaqII GACCGA 1 cut(s) 4342
TatI WGTACW 4 cut(s) 177, 1876, 2452, 4624
TauI GCSGC 5 cut(s) 667, 1282, 1766, 2169, 4881
TseFI GTSAC 3 cut(s) 745, 2837, 2964
Tsp45I GTSAC 3 cut(s) 745, 2837, 2964
TspGWI ACGGA 5 cut(s) 1936, 2482, 4582, 4633, 4694
TspMI CCCGGG 2 cut(s) 222, 962
Tth111I GACNNNGTC 2 cut(s) 593, 3593
Vha464I CTTAAG 2 cut(s) 3377, 4490
VspI ATTAAT 2 cut(s) 1113, 3173
XagI CCTNNNNNAGG 1 cut(s) 3149
XbaI TCTAGA 1 cut(s) 1202
XceI RCATGY 2 cut(s) 628, 2604
XhoI CTCGAG 1 cut(s) 3648
XmaI CCCGGG 2 cut(s) 222, 962
XmiI GTMKAC 4 cut(s) 326, 1545, 2865, 3990
XmnI GAANNNNTTC 6 cut(s) 44, 84, 1053, 1298, 3321, 5136
XspI CTAG 8 cut(s) 917, 1161, 1203, 2655, 2987, 4727, 5063, 5167
ZraI GACGTC 1 cut(s) 2041
ZrmI AGTACT 1 cut(s) 179
Zsp2I ATGCAT 5 cut(s) 465, 630, 976, 1120, 1505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.