Prupe.5G145600_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000526
Physical Location & Seq
Forward (+)
13378962 .. 13386044
7083 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G145600.1

Sequence Viewer

Length: 5121 bp
ATGGCAACACGGAGAGAGCACATAGAAGAAATACGCATGACAAAGTTCTCCATTGGAGGTGAACTCAACCCTTTAACTGAAGATCTTCACCAGGCCGTCAAGAATCTCTCTGCTGAGCTCTATGCTAAAGATGTTCACTTTCTCATGGAACTCATCCAGAATGCAGAAGATAATGAGTACTCAGAGGGGGTTGATCCATCACTTGAGTTTGTCATAACATCCCGGGATATAACCGGCACAGGGGCTCCTGCCACATTGCTCGTTTTCAATAATGAGAAGGGATTTTCTCCTAAAAACATAGAGTCTATTTGCAGTATTGGACGGTCCACCAAGAAAGGCAACAGGAAGCGCGGCTATATTGGGGAGAAAGGAATTGGCTTCAAGAGCGTGTTTCTGATCACAGCTCAGCCTTACATTTTTAGTAATGGTTATCAAATAAGGTTCAGTGAAGAGCCTTGTATGCATTGCAATCTTGGGTACATAGTTCCTGAATGGGTTGAGGAAAACCCAACTCTGTCTGACATAAGACAGATATATGGTTCTGGTTCTGCCCTTCCAACTACAACACTGATCTTACCTCTAAAGCCTGACAAGGTCAAACCTGTGAAGCAGCAGCTCTCTAAAATGCATCCTGAAGTTCTCTTATTCCTTGCAAAGGTTAAGCGGCTCTCGGTTAGGGAAGTTAATGAGGATCCAAGGCTCAACACTGTAACCGCGATAGCAATATCAAGTGAGACTGATTTTGAGACGCGGAAGAACATTGATGCTGATTCCTACACGCTCCATCTCTCTGCAGAAGAAAATGGTAATGAGTTGGAAACTGAATGCAGCTATTATATGTGGAAGCAGAAGTTTCCTGTCAAGCAAGACTGCAGAGATGAGAAGAGAATGGAGGTAGACGAGTGGGTGATCACGCTAGCATTTCCATATGGAGAACGCCTTAATAGAGGAACAAGCTCACCCGGGATCTATGCATTCCTTCCCACCGAAATGATTACAAATTTGCCTTTCATAATTCAGGCAGATTTTCTTTTAGCATCATCAAGAGAAAATATTCTATTGGACAAGAAGTGGAATCAAGGAATTCTGAACTGTGTGCCCTCTGCATTTATTAATGCATTTCTCTCGCTCGTGAGAACTGTAGAAGATGCGCCGGTATCTAGTTTGCCTCCCTTTTTCAGGTTCTTACCTGTCCAGAGCTCTCACTATTATGAATTGAATGTTGTGAGGGAGTCGATCAAAGCACGGTTGGTGGAGGAAGATATTGTACCATGTGAGCCGCACAAGGGGCAGAAGTTCTTCCATAAACCTCGTGAAGTGGGTAGGTTATTGCCTGCTTTTTGGAATATTTTGAGAAAGGCAAGGGAAGTAGGGGTAAGCTTGCCGAATCTCTCTTCCCATGGAAAATATATTTTGTGTCATTCATTTGACAAAAAGGAGTATGATCACATTCTGAGTTTCCTTGGAGTTGAACCAGTGGATGATGAATGGTATGCTAAATGCATTCAGAGTTCCAATCTGGTAGTTGGGGTTCCAGAAGATGTCTACTTGGAACTTCTACTGTTTATTGCTGATAATTGGGGGCCCAAATTTCATTGCACCACCATCAAGTACATACCACTAATAAAATTTGTGGATCTTTATGAGCGTGCTTCTTTGTGCAGCTTGAGCTCAATGCGGACTGGTGAAAAGAAGGTTCGCCTATCCCATCATTTTTGGGAGGTTTCATGGCTGATTGACTGGAACCGTGAATTTATATCTGTGGCAAGTCTTCTTTTTATGCCAAAAAGAACCCAAGAAGCTATCCAGTCATGTCCTAACAAGGACAAGTTGGTGAAATGGCTTGCGGAAGAAATGAAGGTTGATACTTTAAATGTACACGAATATGCAGTTTGCCTCTATAATTCTCTTGGTAATGAACGGAAACCTGCTGTTGCATACGCCCACTTTTTGTATCATTCATTCCACAAGGGCCATATATCTTATCTCGAAGTTGTTGATTTATGTGGTAAGATGCCACTCGTGAATAACTACGGGTATGTCATCAGACAAAAGACAGGGGTTATTGTCCCTGCCAATGAAAGCAAGTGGGCTGGGTTGACTGATTCAAATCTATGGACAGAAGAAGGCTATGTTGAGTTAGGAGAAGAGTACATGAATCCAGGCTGCTTTGCAGGTAAAGTTACTGAACCGAAGCAGCTCCTTGAGTTCCTCAAAGTTCCTACTGGAGCTTCGGATGTCCCTTATATTTCTGCTCCCAATGCCTGTATTCCAACTGTATCTGCAACACTCACTAAGCAAAATGCATTCTTGCTTTTGGAATGGATTCGTCACCTGAGATACCAAAGGGTTCACATCCCGGAGAAGTTCTTGAAATGTATAAAGGAAGGTAGCTGGTTGAAAGTTACTTTGAATGGTTTTTCTGCGTCCAGGCCACCTTCGCAGTCATTTGTACTTACACCATCATGGGGAAACATTTTGCAGAACGGATCCGCTTTTGTTGATATCCCGTTGGTTGACCAGAGTTATTATGGTGAGAGAATTGATGGTTACAAGGAAGAGCTGAAAACAATTGGAGTGATGTTTGAATTTGGAGAAGCATGTGAGTTTATTGGGAAGCATCTTATGTCTCTGGCAGCGTCGTCCACTTTAACTAGAGGCAACGTACTTTCAATACTACAATTTATCAAGCTCTTGAGGGACAAATGTCTTCCCCCAGACGATTTCATCCGCAGTATCAGAAAAGGACAATGGCTTAAAACAAAATCTCATGGTTATAGGTCTCCTGTTGGATCTGTCTTGTTTGACCAAGAGTGGAGAATTGCGTCCAAAATAAGTGACATTCCCTTCATTGATCAGGAGTTATATGGTGAAGAAATCTTCCGTTTCAAAACAGAACTTGAGTTGCTTGGTGTGGTTGTCTCCTTCAAAAGAAATTACCATCTTATCATCGACCACCTTAAGTCACCTGCACGCTTAACTGCTCTGCCACCTGAGGCTGTTCTACTAATGCTTCAAATTATGCTTATTTCAAATTCATCTGATAAGATTGTCGAAGCATTGAAAGGAGCGAAATGCCTGAAGACAAATAATGGTTACAAGTCTCCACGTGAATGTCTCTTGTTTCACCCTGAATGGGGTTGCCTTCTTCAGGTCCTTAGTGGCCTCCCATTGATTGATCATAATTTTTACGGAAGCAGAATCTTTAACTACAGAGACGAGTTGAGAAAGATAGGGGCAGTTGTTGACTTTGAAGAGGCTGCAAAAGTATTTGCTCGCCATTTCCGGCAGGCTTCCATCATCACCAAAGAAAATGTTTCATCATTTTTGTCCTGTTACAGAAAGCTGAAGGGCACTGAGTTTAGATTTCCTGCGGATCTTAAGAGTTGCATTCGCGAAGAAAAGTGGCTGCGTACTCGCCCTGGTGTTTATAGATCTCCAAGACAGTGCATTCTCTATAGTCCTAATTGGGATTCTATCTCTCCAATATGTCCTCTTCTCCCTTTTATTGACGACTCTAATAATTGGTATGGCAAGAACATTCATGAATATAAGGAAGAGTTGAAGAGCTTGGGGGTTGTTGTGGAATTCAAGGACGGGGTCCAGTTTGTGCCTTCTGGTCTTCAGCTTCCCAAGAATCTGAGCTGCATTTCTCGAGGAAACGCACTTGCTTTGCTGGAATGCATAAGGATTCTATTGCAGGAGAAAGATTACTCCTTTCCTGATGCGTTCATGAAGGAAGTTTCTCAGGCATGGTTAAAGACTGGTGCTGGTTATAGGCTTCCCACCCAGTGCTTGTTGTTTGATTCCAAGTTTGGTGAGTATCTAAAGCAGACTGATGGGCCTTTCATTGATGTAGAATTTTATGGTTGTAAAATTGCAACATACAGACAAGAGCTGTCTGCAATAGGAGTGATTGTTGAAGCGGCAGAAGGATGTCCTTTGATTGCCAGCCAGCTTTATCTTCATGATGAGTTTTCCACTTTCGTTCGAGTATATAATTACTTGAGTGAGTTTAAGTGGGAGCCAGATAGTGAGGCTGACAGATGGATCTGGATTCCGAAGGGAGATCAGAATGGAGATTGGGTTAATCCAGATGACTGTGTTGTATATGACAAGGATGATCTATTTGGTTCGCAGTTGACTGTTCTGAAGAACTACTTTGAGCATAACCTACTCGTGTTCTTCTCCAGGGCTTACAGGGTAAAGTCCCGTCCTTCAATTGATGACTACTGCGAGCTCTGGAAGGCGTGGGAAACTTCTGAAACTGGATTGTCGCAGGATCAATGTTGCAAGTTCTGGCGGTATGTTTCAAAGAACTGGAATGCAAAAACTGAGAAAGCTCTCCCTGAGGCCTTGTTGAAAATACCTGTTAATTCAGGCTCAGATGAAATCGTTTTGTTAAACAAGTGCGATGTTTTCCTTCCAGATGACCTTCAACTTAAGGATCTTTTTGAACAGTCTTCTCCTGACCCCGTCTTTGTTTGGTATCCTCAGCCAAGCTTGCCTGACTTGCCTCGGACTACATTGCTTGAAATGTATAGGAAAATTGGTGTTCGTACTATTTCTGAATCTGTACAGAAGGAAGAACTGTCCCTCGAAAATAGTGTTGATCAACAGGTAATTCCAACAGAGAAATTGATCGGGAAAGTGCTACTCCGGCTGATTCTTGGTTTTCTAGCTTGCCCTCCAATTGAAATGGAAGCAGGGACGAGGCGAAAAGCTGTCCAAGGTCTTCTCAGTCTCACTGTTGTAGAGACAACTGAACCGATCACTGTAAACTATAACTTACCATTGTCTTCTGGGGAGACTTTGAATGTCAGAGCAAGCCGCAAGATAAGATGGGACAGAGAGATGTCCAAATTTTTCACTCAGAAAATTGACAGGTCTGGGGGACATAAAAGTATCGTTGAGTTTGCCACATATTTCTCTGAAGTAATATCTGATGGTGTCTTGTGGGAGCACACTGATCATATCCCTGCACTTTCTGAGCTGATCAAGTTGGCCTTTGTGCTAGAATTTAATGAGGAAGCAGTTGATTTTTTGATGAAGTCCAAGAATTTGCAAATCTTCATTGAGGATGAAGAGTTTCTCAATTCTGCCTTCCCTTCTTCCACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1707

Amino Acids

195.05

Weight (kDa)

5.96

Isoelectric Point (pI)

44.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28020 AT3G48770 AT3G48770
fragaria_vesca FvH4_5g09020 FvH4_5g09020
malus_domestica MD06G1092400.v1.1 MD06G1134600.v1.1 MD06G1134700.v1.1 MD06G1134800.v1.1 MD06G1135200.v1.1 MD06G1135400.v1.1 MD06G1135500.v1.1 MD08G1137600.v1.1 MD15G1395100.v1.1
prunus_persica Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145600_v2.0.a1 Prupe.5G145800_v2.0.a1 Prupe.5G145900_v2.0.a1
pyrus_communis pycom06g12550 pycom06g12570 pycom06g12580 pycom06g12590 pycom06g12610 pycom06g12630 pycom06g12640 pycom06g12650 pycom08g06280
rosa_chinensis RchiOBHm_Chr6g0249321 RchiOBHm_Chr6g0275111 RchiOBHm_Chr7g0189191 RchiOBHm_Chr7g0189261
rosa_laevigata RLG00000004622 RLG00000004623 RLG00000008237 RLG00000008238 RLG00000023303 RLG00000035187
rosa_multiflora Rmu_co8294311.1_g000001 Rmu_co8389833.1_g000001 Rmu_sc0001044.1_g000004 Rmu_sc0001329.1_g000011 Rmu_sc0006012.1_g000010 Rmu_sc0007286.1_g000032 Rmu_sc0009360.1_g000016 Rmu_sc0011817.1_g000001 Rmu_sc0015050.1_g000001
rosa_roxburghii Rroxscaffold_1G00064000 Rroxscaffold_3G00265460 Rroxscaffold_3G00265580 Rroxscaffold_3G00265640 Rroxscaffold_3G00265690 Rroxscaffold_3G00265700 Rroxscaffold_7G00193620 Rroxscaffold_7G00204550 Rroxscaffold_7G00217350
rosa_rugosa Rorug01G0465500 Rorug06G0088500 Rorug06G0496700 Rorug06G0496700
rosa_samantha Rh1AG064800 Rh2BG015100 Rh2BG493500 Rh2DG017300 Rh2DG502500 Rh4BG208500 Rh5BG528500 Rh5CG552900 Rh6CG207800 Rh6DG197900 Rh6DG198000 Rh7AG101300 Rh7AG101400 Rh7AG101600 Rh7BG104100 Rh7BG104200 Rh7CG105600 Rh7CG105700 Rh7DG103200 Rh7DG103400
rosa_wichuraiana Rw1G007300 Rw5G034670 Rw7G008750 Rw7G008780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2977
Acc36I ACCTGC 3 cut(s) 1936, 2165, 2977
AccI GTMKAC 2 cut(s) 897, 1545
AccII CGCG 4 cut(s) 351, 716, 751, 3396
AcuI CTGAAG 8 cut(s) 99, 654, 3101, 3134, 3368, 3608, 4172, 4953
AcvI CACGTG 1 cut(s) 3110
AdeI CACNNNGTG 1 cut(s) 3792
AfiI CCNNNNNNNGG 8 cut(s) 240, 655, 1179, 1286, 2468, 3136, 3137, 3151
AflII CTTAAG 3 cut(s) 2960, 3380, 4442
AjnI CCWGG 5 cut(s) 90, 2161, 2429, 3421, 4190
Alw21I GWGCWC 6 cut(s) 21, 120, 1202, 1673, 4242, 4965
Ama87I CYCGRG 3 cut(s) 222, 962, 3654
AoxI GGCC 8 cut(s) 93, 1583, 1972, 2432, 3163, 3842, 4353, 5004
ApaI GGGCCC 1 cut(s) 1587
ArsI GACNNNNNNTTYG 2 cut(s) 4463, 4495
AseI ATTAAT 1 cut(s) 1113
Asp700I GAANNNNTTC 5 cut(s) 84, 1053, 1298, 2325, 5088
AspLEI GCGC 2 cut(s) 351, 1153
AspS9I GGNCC 7 cut(s) 324, 1583, 1584, 1972, 3154, 3601, 3842
AsuC2I CCSGG 5 cut(s) 223, 224, 963, 964, 2360
AsuNHI GCTAGC 1 cut(s) 916
AvaI CYCGRG 3 cut(s) 222, 962, 3654
AvaII GGWCC 3 cut(s) 324, 3154, 3601
AxyI CCTNAGG 2 cut(s) 2994, 4350
BaeGI GKGCMC 3 cut(s) 1101, 1587, 3356
BamHI GGATCC 2 cut(s) 691, 2489
BanII GRGCYC 6 cut(s) 120, 247, 1202, 1587, 1673, 4242
BarI GAAGNNNNNNTAC 2 cut(s) 1251, 1283
BauI CACGAG 4 cut(s) 1130, 1311, 2021, 4178
BbrPI CACGTG 1 cut(s) 3110
BbsI GAAGAC 7 cut(s) 1763, 2702, 3089, 3614, 4455, 4727, 4791
Bbv12I GWGCWC 6 cut(s) 21, 120, 1202, 1673, 4242, 4965
BbvCI CCTCAGC 1 cut(s) 4494
BceAI ACGGC 1 cut(s) 80
BcgI CGANNNNNNTGC 2 cut(s) 1107, 1141
BciT130I CCWGG 5 cut(s) 92, 2163, 2431, 3423, 4192
BciVI GTATCC 1 cut(s) 4500
BclI TGATCA 8 cut(s) 396, 909, 1444, 2854, 3178, 4612, 4969, 4995
BcnI CCSGG 5 cut(s) 223, 224, 963, 964, 2360
BfaI CTAG 6 cut(s) 917, 1161, 2655, 4679, 5015, 5119
BfmI CTRYAG 5 cut(s) 792, 871, 1140, 3211, 3457
BfrI CTTAAG 3 cut(s) 2960, 3380, 4442
BfuAI ACCTGC 3 cut(s) 1936, 2165, 2977
BfuI GTATCC 1 cut(s) 4500
BglII AGATCT 2 cut(s) 82, 3434
BlpI GCTNAGC 2 cut(s) 114, 405
BmcAI AGTACT 1 cut(s) 179
Bme18I GGWCC 3 cut(s) 324, 3154, 3601
BmeT110I CYCGRG 3 cut(s) 222, 962, 3654
BmgT120I GGNCC 7 cut(s) 324, 1583, 1584, 1972, 3154, 3601, 3842
BmiI GGNNCC 9 cut(s) 246, 693, 1533, 1584, 1585, 1745, 2491, 3602, 4026
BmrI ACTGGG 1 cut(s) 3784
BmsI GCATC 7 cut(s) 637, 754, 1046, 1138, 2004, 2629, 3715
BmtI GCTAGC 1 cut(s) 920
BmuI ACTGGG 1 cut(s) 3784
BoxI GACNNNNGTC 1 cut(s) 2706
BpiI GAAGAC 7 cut(s) 1763, 2702, 3089, 3614, 4455, 4727, 4791
BplI GAGNNNNNCTC 2 cut(s) 48, 80
BpmI CTGGAG 2 cut(s) 2247, 4174
Bpu10I CCTNAGC 1 cut(s) 4494
Bpu1102I GCTNAGC 2 cut(s) 114, 405
BpuEI CTTGAG 6 cut(s) 224, 1687, 2225, 2716, 2921, 4027
BpuMI CCSGG 5 cut(s) 223, 224, 963, 964, 2360
BsaAI YACGTR 1 cut(s) 3110
BsaBI GATNNNNATC 1 cut(s) 2109
BsaI GGTCTC 1 cut(s) 2787
BsaJI CCNNGG 9 cut(s) 222, 695, 962, 1399, 1462, 3421, 4191, 4517, 4729
BsaXI ACNNNNNCTCC 6 cut(s) 229, 259, 3903, 3933, 4071, 4101
Bsc4I CCNNNNNNNGG 8 cut(s) 240, 655, 1179, 1286, 2468, 3136, 3137, 3151
Bse118I RCCGGY 2 cut(s) 233, 1153
Bse21I CCTNAGG 2 cut(s) 2994, 4350
Bse3DI GCAATG 4 cut(s) 254, 463, 1594, 4526
Bse8I GATNNNNATC 1 cut(s) 2109
BseBI CCWGG 5 cut(s) 92, 2163, 2431, 3423, 4192
BseDI CCNNGG 9 cut(s) 222, 695, 962, 1399, 1462, 3421, 4191, 4517, 4729
BseJI GATNNNNATC 1 cut(s) 2109
BseLI CCNNNNNNNGG 8 cut(s) 240, 655, 1179, 1286, 2468, 3136, 3137, 3151
BseMI GCAATG 4 cut(s) 254, 463, 1594, 4526
BseSI GKGCMC 3 cut(s) 1101, 1587, 3356
BseYI CCCAGC 1 cut(s) 2093
BsgI GTGCAG 3 cut(s) 1681, 2955, 4965
Bsh1236I CGCG 4 cut(s) 351, 716, 751, 3396
BshFI GGCC 8 cut(s) 95, 1585, 1974, 2434, 3165, 3844, 4355, 5006
BsiHKAI GWGCWC 6 cut(s) 21, 120, 1202, 1673, 4242, 4965
BsiHKCI CYCGRG 3 cut(s) 222, 962, 3654
BsiSI CCGG 7 cut(s) 223, 234, 963, 1154, 2360, 3286, 4660
BslFI GGGAC 8 cut(s) 2054, 2225, 2714, 4195, 4579, 4723, 4859, 4908
BslI CCNNNNNNNGG 8 cut(s) 240, 655, 1179, 1286, 2468, 3136, 3137, 3151
BsmBI CGTCTC 2 cut(s) 740, 3210
BsmFI GGGAC 8 cut(s) 2054, 2225, 2714, 4195, 4579, 4723, 4859, 4908
BsmI GAATGC 9 cut(s) 166, 830, 974, 1503, 2306, 3390, 3450, 3686, 4330
BsnI GGCC 8 cut(s) 95, 1585, 1974, 2434, 3165, 3844, 4355, 5006
Bso31I GGTCTC 1 cut(s) 2787
BsoBI CYCGRG 3 cut(s) 222, 962, 3654
Bsp120I GGGCCC 1 cut(s) 1583
Bsp1407I TGTACA 2 cut(s) 1876, 4576
Bsp1720I GCTNAGC 2 cut(s) 114, 405
Bsp19I CCATGG 1 cut(s) 1399
Bsp68I TCGCGA 1 cut(s) 3396
BspANI GGCC 8 cut(s) 95, 1585, 1974, 2434, 3165, 3844, 4355, 5006
BspFNI CGCG 4 cut(s) 351, 716, 751, 3396
BspHI TCATGA 3 cut(s) 3544, 3732, 3967
BspLI GGNNCC 9 cut(s) 246, 693, 1533, 1584, 1585, 1745, 2491, 3602, 4026
BspMAI CTGCAG 2 cut(s) 796, 875
BspMI ACCTGC 3 cut(s) 1936, 2165, 2977
BspOI GCTAGC 1 cut(s) 920
BspQI GCTCTTC 3 cut(s) 444, 2553, 3560
BspTI CTTAAG 3 cut(s) 2960, 3380, 4442
BspTNI GGTCTC 1 cut(s) 2787
BsrDI GCAATG 4 cut(s) 254, 463, 1594, 4526
BsrFI RCCGGY 2 cut(s) 233, 1153
BsrGI TGTACA 2 cut(s) 1876, 4576
BssAI RCCGGY 2 cut(s) 233, 1153
BssECI CCNNGG 9 cut(s) 222, 695, 962, 1399, 1462, 3421, 4191, 4517, 4729
BssSI CACGAG 4 cut(s) 1130, 1311, 2021, 4178
BssT1I CCWWGG 4 cut(s) 695, 1399, 1462, 4729
Bst2BI CACGAG 4 cut(s) 1130, 1311, 2021, 4178
Bst2UI CCWGG 5 cut(s) 92, 2163, 2431, 3423, 4192
BstAFI CTTAAG 3 cut(s) 2960, 3380, 4442
BstAUI TGTACA 2 cut(s) 1876, 4576
BstBAI YACGTR 1 cut(s) 3110
BstDSI CCRYGG 1 cut(s) 1399
BstENI CCTNNNNNAGG 3 cut(s) 653, 1177, 3149
BstFNI CGCG 4 cut(s) 351, 716, 751, 3396
BstHHI GCGC 2 cut(s) 351, 1153
BstMWI GCNNNNNNNGC 9 cut(s) 384, 460, 1288, 1668, 2261, 2440, 4504, 4513, 4660
BstNI CCWGG 5 cut(s) 92, 2163, 2431, 3423, 4192
BstNSI RCATGY 1 cut(s) 2604
BstPAI GACNNNNGTC 1 cut(s) 2706
BstSFI CTRYAG 5 cut(s) 792, 871, 1140, 3211, 3457
BstSLI GKGCMC 3 cut(s) 1101, 1587, 3356
BstUI CGCG 4 cut(s) 351, 716, 751, 3396
BstV2I GAAGAC 7 cut(s) 1763, 2702, 3089, 3614, 4455, 4727, 4791
Bsu36I CCTNAGG 2 cut(s) 2994, 4350
BsuI GTATCC 1 cut(s) 4500
BsuRI GGCC 8 cut(s) 95, 1585, 1974, 2434, 3165, 3844, 4355, 5006
BtgI CCRYGG 1 cut(s) 1399
BtgZI GCGATG 1 cut(s) 4428
BtuMI TCGCGA 1 cut(s) 3396
BveI ACCTGC 3 cut(s) 1936, 2165, 2977
CciI TCATGA 3 cut(s) 3544, 3732, 3967
CfoI GCGC 2 cut(s) 351, 1153
Cfr10I RCCGGY 2 cut(s) 233, 1153
Cfr13I GGNCC 7 cut(s) 324, 1583, 1584, 1972, 3154, 3601, 3842
Cfr9I CCCGGG 2 cut(s) 222, 962
CseI GACGC 4 cut(s) 757, 2415, 2628, 2814
DraI TTTAAA 1 cut(s) 1872
DraIII CACNNNGTG 1 cut(s) 3792
Ecl136II GAGCTC 4 cut(s) 118, 1200, 1671, 4240
Eco130I CCWWGG 4 cut(s) 695, 1399, 1462, 4729
Eco147I AGGCCT 1 cut(s) 4355
Eco24I GRGCYC 6 cut(s) 120, 247, 1202, 1587, 1673, 4242
Eco31I GGTCTC 1 cut(s) 2787
Eco32I GATATC 1 cut(s) 2506
Eco47I GGWCC 3 cut(s) 324, 3154, 3601
Eco53kI GAGCTC 4 cut(s) 118, 1200, 1671, 4240
Eco57I CTGAAG 8 cut(s) 99, 654, 3101, 3134, 3368, 3608, 4172, 4953
Eco72I CACGTG 1 cut(s) 3110
Eco81I CCTNAGG 2 cut(s) 2994, 4350
Eco88I CYCGRG 3 cut(s) 222, 962, 3654
EcoICRI GAGCTC 4 cut(s) 118, 1200, 1671, 4240
EcoNI CCTNNNNNAGG 3 cut(s) 653, 1177, 3149
EcoO109I RGGNCCY 2 cut(s) 1583, 3154
EcoRI GAATTC 2 cut(s) 1083, 3587
EcoRII CCWGG 5 cut(s) 90, 2161, 2429, 3421, 4190
EcoRV GATATC 1 cut(s) 2506
EcoT14I CCWWGG 4 cut(s) 695, 1399, 1462, 4729
EcoT22I ATGCAT 7 cut(s) 465, 630, 976, 1120, 1505, 2308, 3686
EcoT38I GRGCYC 6 cut(s) 120, 247, 1202, 1587, 1673, 4242
ErhI CCWWGG 4 cut(s) 695, 1399, 1462, 4729
Esp3I CGTCTC 2 cut(s) 740, 3210
FalI AAGNNNNNCTT 4 cut(s) 4145, 4177, 4991, 5023
FaqI GGGAC 8 cut(s) 2054, 2225, 2714, 4195, 4579, 4723, 4859, 4908
FauNDI CATATG 1 cut(s) 928
FbaI TGATCA 8 cut(s) 396, 909, 1444, 2854, 3178, 4612, 4969, 4995
FblI GTMKAC 2 cut(s) 897, 1545
FriOI GRGCYC 6 cut(s) 120, 247, 1202, 1587, 1673, 4242
FspBI CTAG 6 cut(s) 917, 1161, 2655, 4679, 5015, 5119
GlaI GCGC 2 cut(s) 350, 1152
GsaI CCCAGC 1 cut(s) 2097
GsuI CTGGAG 2 cut(s) 2247, 4174
HaeIII GGCC 8 cut(s) 95, 1585, 1974, 2434, 3165, 3844, 4355, 5006
HapII CCGG 7 cut(s) 223, 234, 963, 1154, 2360, 3286, 4660
HgaI GACGC 4 cut(s) 757, 2415, 2628, 2814
HhaI GCGC 2 cut(s) 351, 1153
Hin6I GCGC 2 cut(s) 349, 1151
HinP1I GCGC 2 cut(s) 349, 1151
HincII GTYRAC 4 cut(s) 2100, 2518, 3247, 4143
HindII GTYRAC 4 cut(s) 2100, 2518, 3247, 4143
HindIII AAGCTT 2 cut(s) 1378, 4501
HpaII CCGG 7 cut(s) 223, 234, 963, 1154, 2360, 3286, 4660
Hpy99I CGWCG 1 cut(s) 2644
HpyCH4IV ACGT 2 cut(s) 2664, 3109
HpyF10VI GCNNNNNNNGC 9 cut(s) 384, 460, 1288, 1668, 2261, 2440, 4504, 4513, 4660
HpySE526I ACGT 2 cut(s) 2664, 3109
HspAI GCGC 2 cut(s) 349, 1151
Ksp22I TGATCA 8 cut(s) 396, 909, 1444, 2854, 3178, 4612, 4969, 4995
LguI GCTCTTC 3 cut(s) 444, 2553, 3560
LmnI GCTCC 8 cut(s) 250, 786, 2205, 2228, 2260, 3068, 4024, 4960
LweI GCATC 7 cut(s) 637, 754, 1046, 1138, 2004, 2629, 3715
MaeI CTAG 6 cut(s) 917, 1161, 2655, 4679, 5015, 5119
MaeII ACGT 2 cut(s) 2664, 3109
MaeIII GTNAC 9 cut(s) 709, 2182, 2330, 2404, 2549, 2837, 2964, 3095, 3335
MfeI CAATTG 3 cut(s) 2571, 4221, 4692
MlyI GAGTC 3 cut(s) 311, 1241, 3509
MmeI TCCRAC 5 cut(s) 581, 795, 2297, 2770, 4652
Mph1103I ATGCAT 7 cut(s) 465, 630, 976, 1120, 1505, 2308, 3686
MroXI GAANNNNTTC 5 cut(s) 84, 1053, 1298, 2325, 5088
MslI CAYNNNNRTG 5 cut(s) 989, 1209, 1884, 2464, 3112
MspCI CTTAAG 3 cut(s) 2960, 3380, 4442
MspI CCGG 7 cut(s) 223, 234, 963, 1154, 2360, 3286, 4660
MunI CAATTG 3 cut(s) 2571, 4221, 4692
Mva1269I GAATGC 9 cut(s) 166, 830, 974, 1503, 2306, 3390, 3450, 3686, 4330
MvaI CCWGG 5 cut(s) 92, 2163, 2431, 3423, 4192
MvnI CGCG 4 cut(s) 351, 716, 751, 3396
MwoI GCNNNNNNNGC 9 cut(s) 384, 460, 1288, 1668, 2261, 2440, 4504, 4513, 4660
NciI CCSGG 5 cut(s) 223, 224, 963, 964, 2360
NcoI CCATGG 1 cut(s) 1399
NdeI CATATG 1 cut(s) 928
NheI GCTAGC 1 cut(s) 916
NlaIV GGNNCC 9 cut(s) 246, 693, 1533, 1584, 1585, 1745, 2491, 3602, 4026
NmuCI GTSAC 3 cut(s) 2330, 2837, 2964
NruI TCGCGA 1 cut(s) 3396
NsiI ATGCAT 7 cut(s) 465, 630, 976, 1120, 1505, 2308, 3686
NspI RCATGY 1 cut(s) 2604
PaeR7I CTCGAG 1 cut(s) 3654
PagI TCATGA 3 cut(s) 3544, 3732, 3967
PaqCI CACCTGC 1 cut(s) 2977
PceI AGGCCT 1 cut(s) 4355
PciSI GCTCTTC 3 cut(s) 444, 2553, 3560
PctI GAATGC 9 cut(s) 166, 830, 974, 1503, 2306, 3390, 3450, 3686, 4330
PdmI GAANNNNTTC 5 cut(s) 84, 1053, 1298, 2325, 5088
PflFI GACNNNGTC 3 cut(s) 593, 3599, 4475
PfoI TCCNGGA 1 cut(s) 2358
PleI GAGTC 3 cut(s) 310, 1240, 3509
PmaCI CACGTG 1 cut(s) 3110
PmlI CACGTG 1 cut(s) 3110
PpsI GAGTC 3 cut(s) 310, 1240, 3509
Ppu21I YACGTR 1 cut(s) 3110
PpuMI RGGWCCY 1 cut(s) 3154
PshAI GACNNNNGTC 1 cut(s) 2706
PshBI ATTAAT 1 cut(s) 1113
Psp124BI GAGCTC 4 cut(s) 120, 1202, 1673, 4242
Psp5II RGGWCCY 1 cut(s) 3154
Psp6I CCWGG 5 cut(s) 90, 2161, 2429, 3421, 4190
PspCI CACGTG 1 cut(s) 3110
PspFI CCCAGC 1 cut(s) 2093
PspGI CCWGG 5 cut(s) 90, 2161, 2429, 3421, 4190
PspN4I GGNNCC 9 cut(s) 246, 693, 1533, 1584, 1585, 1745, 2491, 3602, 4026
PspOMI GGGCCC 1 cut(s) 1583
PspPI GGNCC 7 cut(s) 324, 1583, 1584, 1972, 3154, 3601, 3842
PspPPI RGGWCCY 1 cut(s) 3154
PsrI GAACNNNNNNTAC 2 cut(s) 1515, 1547
PstI CTGCAG 2 cut(s) 796, 875
PsyI GACNNNGTC 3 cut(s) 593, 3599, 4475
RruI TCGCGA 1 cut(s) 3396
RseI CAYNNNNRTG 5 cut(s) 989, 1209, 1884, 2464, 3112
SacI GAGCTC 4 cut(s) 120, 1202, 1673, 4242
SapI GCTCTTC 3 cut(s) 444, 2553, 3560
Sau96I GGNCC 7 cut(s) 324, 1583, 1584, 1972, 3154, 3601, 3842
ScaI AGTACT 1 cut(s) 179
SchI GAGTC 3 cut(s) 311, 1241, 3509
SfaNI GCATC 7 cut(s) 637, 754, 1046, 1138, 2004, 2629, 3715
SfcI CTRYAG 5 cut(s) 792, 871, 1140, 3211, 3457
Sfr274I CTCGAG 1 cut(s) 3654
SinI GGWCC 3 cut(s) 324, 3154, 3601
SlaI CTCGAG 1 cut(s) 3654
SmaI CCCGGG 2 cut(s) 224, 964
SmiMI CAYNNNNRTG 5 cut(s) 989, 1209, 1884, 2464, 3112
SseBI AGGCCT 1 cut(s) 4355
SspI AATATT 2 cut(s) 1054, 1348
SspMI CTAG 6 cut(s) 917, 1161, 2655, 4679, 5015, 5119
SstI GAGCTC 4 cut(s) 120, 1202, 1673, 4242
StuI AGGCCT 1 cut(s) 4355
StyI CCWWGG 4 cut(s) 695, 1399, 1462, 4729
TaiI ACGT 2 cut(s) 2667, 3112
TaqI TCGA 7 cut(s) 1235, 1989, 2952, 3054, 3655, 3991, 4599
TatI WGTACW 6 cut(s) 177, 1611, 1876, 2151, 2452, 4576
TauI GCSGC 5 cut(s) 354, 667, 1282, 3929, 4833
TseFI GTSAC 3 cut(s) 2330, 2837, 2964
Tsp45I GTSAC 3 cut(s) 2330, 2837, 2964
TspGWI ACGGA 5 cut(s) 25, 1936, 2502, 2873, 3207
TspMI CCCGGG 2 cut(s) 222, 962
Tth111I GACNNNGTC 3 cut(s) 593, 3599, 4475
Vha464I CTTAAG 3 cut(s) 2960, 3380, 4442
VpaK11BI GGWCC 3 cut(s) 324, 3154, 3601
VspI ATTAAT 1 cut(s) 1113
XagI CCTNNNNNAGG 3 cut(s) 653, 1177, 3149
XceI RCATGY 1 cut(s) 2604
XcmI CCANNNNNNNNNTGG 1 cut(s) 2528
XhoI CTCGAG 1 cut(s) 3654
XmaI CCCGGG 2 cut(s) 222, 962
XmiI GTMKAC 2 cut(s) 897, 1545
XmnI GAANNNNTTC 5 cut(s) 84, 1053, 1298, 2325, 5088
XspI CTAG 6 cut(s) 917, 1161, 2655, 4679, 5015, 5119
ZrmI AGTACT 1 cut(s) 179
Zsp2I ATGCAT 7 cut(s) 465, 630, 976, 1120, 1505, 2308, 3686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.