FvH4_5g09020

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000526
Physical Location & Seq
Reverse (-)
5211371 .. 5218560
7190 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g09020.t1

Sequence Viewer

Length: 5094 bp
ATGGCGACGGCAATGACGCCGAGAGAGCACATAGAAGATATACGGAAGACCAAGTTCTCCATCGGAGAAAAGCTCAACCCTTTAACGGAGGATCTTCACCAGGCCGTCAAGAACCTCTCCGGCGAGCTTTACGCTAAAGACGTCCACTTTTTCATGGAACTCGTTCAGAATGCAGAAGACAATGAGTACTTGGAGGGGGTTGATCCATCCCTTGAGTTTGTGATTACTTCCCGCGATATAACAGCTACAGGGGCTCCAGCCACATTGCTGGTTTTCAATAATGAGAAGGGGTTTTCTCCCAAAAACATAGACTCCATTTGCAGTGTTGGACGGTCCACCAAGAAAGGCAACAGGAAGTGTGGTTATATCGGGGAGAAAGGAATTGGCTTCAAGAGTGTGTTCCTGATCACTGCTCAGCCTTACATATTTAGTAATGGTTATCAGATCAGGTTTAGTGAAGAGCCTTGTTCACAATGCAATGTTGGCTACATAGTACCCGAATGGGTTGAGCAGAATCCCACTCTCTCTGATATACAAAAGATATATGGTTCTGCTACTGCCTTTCCTACCACAACACTGGTCTTACCTCTAAAGCTTGACAAGGTTGAAGCTGTGAAGAAGCAACTCTCAATCATTCATCCGGAAGTTCTCCTGTTCCTATCGAAGATTAAACGGCTCTCTGTGAGGGAAGACAAAGAGGATCCAAGGCTGAACACTGTGAGTGCAATTGAGATAGCAAGCCAGACAGATTGGGTTAGCAGGAAGAACATTAATGCTCAATCCTACACTCTGAGTCTTTCAGCTGAAGAAAATAATGGTAAAGGAGAATGCAGTTACTACATGTGGAAACAAACGTTTCCTGTCAGGCGGGAATTCAGAGTTGAGAGGAGAATGGAGGTGGATGAGTGGGTGATCACTCTTGCATTTCCAAATGGAGAACGTCTTCGAAGAGCAATGAACTCATCGCCTGGTGTTTATGCATTTCTTCCCACAGAGATGATCACCAACTTTCCCTTCATAATACAGGCGGATTTTCTTTTAGCATCTTCAAGAGAGACGATCCTACTTGACAACAAGTGGAATCAAGGGATTCTTGAGTGTGTGCCTACTGCATTTTCCCATGCCTTCAACTCTCTTGTGAAAACTGTGGAAGATGCACCGGCATCAAATTTGCCTCCTATGTTCAGATTCTTGCCTGTTCAGGCTTCTTCCTATAAAGAATTAAATGTTGTAAGAGACTCAATAAGAGGGAAGATGATTGACGAAGACATCATACCAGTTGAGCCTGAAATGGAGCAGAGGTTCTTCCACAAGCCTCGTGAAGTGGGAAGACTAATGCCTGCCTTTTGGACTATTTTGAGGAAGGCGAGGGATCAAGGTGTAAGCCTGATCAAGCTTTCATCACATGAAAGATTTGTTCTGTCTTATTCCTTCGACAGTGAGGAATATGATCCTATACTAAGTTTTCTTGGAGTTGAACCAGTGCATGATGATTGGTATGATAAATGCATCCAGGGTACTTCCAATCTTGTTGATGGAGTGTCAGAACATGTGTACTTGGAGCTTCTACTGTTTATTGCTGACAATTGGGGTTCTAAATTCTGCCGCACCAACATGAAGAACATACCATTGATAAAATGCGCAGACTCTTTGTGCAGCATAAGCAAAATCCAGAAGGGTGAATGCAGAGTTTGCTTATCGAGCCATTCTTCTTATGTTTCATGGCTGATTGATTGCAACCGGGAATTTTCATCTGTGACTCGTCTTTCCTTTATGCCGAAAGCCACACAGGAAGCTATCTGGGCATGCGCTAGGAAGGATACGTTGAAGAAATGGCTGGCTGATCAGGTTAAGGTTGGTTCTGTTGATGTCTACAGATATGCACTTGAACTCCTTGGGAATTCTCTTAATGAGCGGAAGCTTGTTGTTGCATATGTTCACTTCTTATATCAGTCATTCAACAATGATTTTCTCTATAGTGGGGATGTTGAGAAGTTATGTGGATGTATGCCACTAGTGGATAGCTACGGGAATGTCATCAAGACTAGAAGAGGGGTTATTGTCCCTGCCAATGGAAGCAAATGGGCGGGATTGACTGATTCCAATCTGTGGAGAAACGAAGGCTATGTTGAATTAGGAGATCATTACATGAATTCTGGCAATTTTGCTGGCAATTTTACACCACAGAAGAAGCTTCTTGAGTTCCTTATAAAATATACTGCTGCTTTGGACGTCCCTCATATTTCTGCTCCAAGTGATGGTATCTCATCTGTATCTGGACCACTTACTAAGCAGAATGCCTTTTTGCTTTTGGATTGGATTCGTCAGCTTAAGTATAAAAGGGTTAACTTCCCTCAGAAGTTTTTGACCTGCATGAAGGAAGGTAGTTGGCTAAAGGTTACTCTGAATGGTTCTCCTGGTTATAGGCCACCATCCCAGTCATTTCTGCTGACCTCATCATGGGGAGATACTCTGCAGAATGGATCCGTTTCTGTTGACATTCCATTGATTGATAAGAGCTTTTATGGCGAGAGCATTAGTGAATACAAGGAGGAGCTAAAGACAATCGGGGTCATGTTTGAATATAGCGAAGCATGTGAATTCATCGGGAAGCATTTCATGTCTCTGGCAGCTTCATCCTCTTTAACAAGAGAAAAAGTATTCTCCATTCTGCGTTTCATCAAGTTTTTAAGGGACAAATGTCTTTCTCCAGCTGATTTTATCTCTGCTATTAAAAAAGGGAACTGGCTGAAGACTTCCCTTGGCTACAGGTCTCCTGTAGGATCTGTTTTATCAGATAAAGAGTGGAATGTTGCCTCCAAAATTAGCAATATTCCCTTCATTGATCAGGACTTCTACGGTGCTGAAATCTGTAATTTCAGAACTGAACTTGAGTTGCTCGGTGCGGTAATCTCCTTCAACAGCAGTTACCAACTTATAATTGACAACCTGAAGTCACCATCTTGCATAACTTCTTGGACACCTGAGGTTGTTATCTTAATGCTCATGTGTATGATTCTTTCTAAATCATCCGATAAACTTGTCAAGGCACTGAAAGGAGCCAAGTGCCTGAAGACAAGTATCGGTTACAAGTGTCCAGGGGAATGTCTCTTGTTTAATCACGAATGGGGTTGCATTCTTCAAGTCATCAGTGGTCTTCCTGTGATTGATCATGAATTCTATGGGAGCAACTTTTTCTTGTACAGAGATGCACTGAGAAAGATTGGAGTGGCGGTTGATTTTGAAGAGGCTGCAAAAGTATTTGCTCAAAATTTCAGGCAGTATGCATCAAAAGCTTCCATCACCAAGGAAAATGTTGCATCATTGCTTTTGTGTTACAGAAAGCTGAAGGGGACTTCATATAAATTTCCTGCTGATCTTAGCAGCTGTATTGGTGAGGCCAAGTGGTTGCGTACTCGTCTCGGTGATTATAGAACTCCAAGGGAATGCATTCTCTTTAGTCCCGAGTGGGAATCTATATCCCCAATAACTCTGCTTCCTTTCATTGATGATTCTGACAGTCACTATGGAAAGAGCATACACGAGTACAGAAAAGAGCTGAAGAGCATGGGGGTTGTCGTAGATTACAAGGAAGGAGCCAAGTTTGTGGCCTCTAGTCTTTATCTCCCTAGGGATTCCAGCCGTATTTTTCCTGTAAATGCACTTGCGTTGCTGGAGTGCATTAGAGTATTACAGGATAAAGGTCACACCTTTCCTCCCGAGTCTGATTTCATGAAGAAAGTTTCTCAAGCATGGATAAAGACTCATGCTGGTTATAGATCACCCAAAGAGTCCTTGCTGTTTGATTCAAAATTTGGCTTATATTTGAAGCGGACTGATGGGCCTTTCATTGATGAGGAGTTTTATGGTGCTAAGATTGCAACATACAAAAAAGAGCTCTCTGCATTGGGTGTTGTTGTTGAAGCTGGAGAAGGATGTTCCTTGATTGCTGGGCACCTTGATAATCATAATGAGACGGAAGCTTTTGTTCGGCTGTATAGTTACTTGAGTGCGTTCAAGTGGGAGCCAGACACTGATGCTGATCGATGGATCTGGAGTCCGAAAGGGGAGTGGGTTCGTCCTGATGCATGTGTTATTTATGACAAGGACGAGCTGTTTGGTTTGCAGTTGACTGTTTTGGAGAAACATTATGGGCATGAACTTCTAACTTTTTTCTCCAGTGCTTTTGGAGTTAAATCCAATCCCACACTTGATGACTACTTGAAGGTTTGGAAGGTTTGGGAAAGTTCTGAAAGTGGTTTGTCATATGCTGATTGTTGCAAGTTCTGGAGCTACGTTTCAAAGCACTGGAATTCAAAAACCGAAAGAACTCTTGCGGATGCCTTGGTGAAGGTACCTGTCAATTCAGGATCTGTTGAAATCTTGTTGTGCAACAAGCGCGATGTTTTCATCGCTGATGACCTTCAATTGAAGTATCTATTCGAGCAGTCTTCTCGTGAATCAATTTTTGTTTGGTACCCTCAGCCTAGTTTGCCTTCCATTCCAAGGACCAAGTTGCTTGATATGTATCGGAAAATTGGTGTTCGCTCTATCTATGAATCTGTACAGAAGGAAGAGTTATCTCTTGCAAATGACGTTCTAGTGGAGTCTTTTCCAAGGGAAAAGTTGATTAAGAAACCGCTTTTCAAACTGATTCTCGGTTTTCTTGCTGCTCCTGCCATGGAAATGGAATCAGATCAGAGGCGCAAAGCTGTTGACGGCCTTCTGAATGTGACTGTGGTGGAGACAACTGAACCAATCACTGTAAGATATAATCTATCATTAACGTCTGGGAAAGTTTTGACTGTGGAAGGAAGCCGAAAGATGCGGTTTGATAAAGAAAATTCAAAGATCTTCACTCAGAAAATGGACAAGTCTGGAGGACAGAAGAGTTCCATTGAGTTTGCTACTTTTTTCTCTGAAGCAATATCTGAGAGTGTGTTATGGGAGAGCACTGATCATATAGATTCACTCTCTGAGCTGATCAAGGTGGCGGCTTTGCTAGACTTCAATGAGGAAGCAGTTGATTTCTTGATGAAAGTCAAGAATCTGGAAACCTTTATGGAGGATGAGGAGTTTCTCAAGACTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1698

Amino Acids

192.57

Weight (kDa)

6.32

Isoelectric Point (pI)

39.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 30 - 146 5.7e-11 Sacsin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28020 AT3G48770 AT3G48770
fragaria_vesca FvH4_5g09020 FvH4_5g09020
malus_domestica MD06G1092400.v1.1 MD06G1134600.v1.1 MD06G1134700.v1.1 MD06G1134800.v1.1 MD06G1135200.v1.1 MD06G1135400.v1.1 MD06G1135500.v1.1 MD08G1137600.v1.1 MD15G1395100.v1.1
prunus_persica Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145600_v2.0.a1 Prupe.5G145800_v2.0.a1 Prupe.5G145900_v2.0.a1
pyrus_communis pycom06g12550 pycom06g12570 pycom06g12580 pycom06g12590 pycom06g12610 pycom06g12630 pycom06g12640 pycom06g12650 pycom08g06280
rosa_chinensis RchiOBHm_Chr6g0249321 RchiOBHm_Chr6g0275111 RchiOBHm_Chr7g0189191 RchiOBHm_Chr7g0189261
rosa_laevigata RLG00000004622 RLG00000004623 RLG00000008237 RLG00000008238 RLG00000023303 RLG00000035187
rosa_multiflora Rmu_co8294311.1_g000001 Rmu_co8389833.1_g000001 Rmu_sc0001044.1_g000004 Rmu_sc0001329.1_g000011 Rmu_sc0006012.1_g000010 Rmu_sc0007286.1_g000032 Rmu_sc0009360.1_g000016 Rmu_sc0011817.1_g000001 Rmu_sc0015050.1_g000001
rosa_roxburghii Rroxscaffold_1G00064000 Rroxscaffold_3G00265460 Rroxscaffold_3G00265580 Rroxscaffold_3G00265640 Rroxscaffold_3G00265690 Rroxscaffold_3G00265700 Rroxscaffold_7G00193620 Rroxscaffold_7G00204550 Rroxscaffold_7G00217350
rosa_rugosa Rorug01G0465500 Rorug06G0088500 Rorug06G0496700 Rorug06G0496700
rosa_samantha Rh1AG064800 Rh2BG015100 Rh2BG493500 Rh2DG017300 Rh2DG502500 Rh4BG208500 Rh5BG528500 Rh5CG552900 Rh6CG207800 Rh6DG197900 Rh6DG198000 Rh7AG101300 Rh7AG101400 Rh7AG101600 Rh7BG104100 Rh7BG104200 Rh7CG105600 Rh7CG105700 Rh7DG103200 Rh7DG103400
rosa_wichuraiana Rw1G007300 Rw5G034670 Rw7G008750 Rw7G008780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2210, 2939
AatII GACGTC 2 cut(s) 144, 2235
Acc16I TGCGCA 1 cut(s) 1642
Acc36I ACCTGC 1 cut(s) 2378
Acc65I GGTACC 2 cut(s) 4354, 4476
AccB1I GGYRCC 3 cut(s) 3954, 4354, 4476
AccB7I CCANNNNNTGG 2 cut(s) 2109, 2258
AccBSI CCGCTC 1 cut(s) 1915
AccI GTMKAC 1 cut(s) 1872
AccII CGCG 2 cut(s) 234, 4401
AccIII TCCGGA 1 cut(s) 640
AclI AACGTT 1 cut(s) 854
AcuI CTGAAG 7 cut(s) 825, 2771, 2972, 3092, 3368, 3581, 4941
AcyI GRCGYC 3 cut(s) 17, 141, 2232
AfiI CCNNNNNNNGG 6 cut(s) 85, 2072, 2109, 2258, 2460, 4506
AflII CTTAAG 1 cut(s) 2330
AflIII ACRYGT 2 cut(s) 840, 1549
AhlI ACTAGT 1 cut(s) 2014
AjnI CCWGG 5 cut(s) 99, 967, 1512, 2416, 3097
AjuI GAANNNNNNNTTGG 2 cut(s) 998, 1030
AloI GAACNNNNNNTCC 2 cut(s) 1286, 1318
Alw21I GWGCWC 3 cut(s) 30, 3900, 4955
Alw26I GTCTC 8 cut(s) 1049, 1230, 2628, 2778, 3113, 3425, 3968, 4739
AlwNI CAGNNNCTG 2 cut(s) 4034, 4373
Ama87I CYCGRG 2 cut(s) 3464, 3719
Aor13HI TCCGGA 1 cut(s) 640
AoxI GGCC 6 cut(s) 102, 2426, 3399, 3609, 3842, 4720
ApeKI GCWGC 6 cut(s) 1656, 2222, 2630, 3251, 3384, 4670
ArsI GACNNNNNNTTYG 2 cut(s) 4100, 4132
AseI ATTAAT 1 cut(s) 771
Asp700I GAANNNNTTC 4 cut(s) 162, 942, 2615, 3450
Asp718I GGTACC 2 cut(s) 4354, 4476
AspA2I CCTAGG 1 cut(s) 3629
AspLEI GCGC 4 cut(s) 1643, 1811, 4401, 4707
AspS9I GGNCC 4 cut(s) 333, 2279, 3842, 4509
AsuC2I CCSGG 1 cut(s) 1742
AsuII TTCGAA 1 cut(s) 946
AvaI CYCGRG 2 cut(s) 3464, 3719
AvaII GGWCC 3 cut(s) 333, 2279, 4509
AvrII CCTAGG 1 cut(s) 3629
AxyI CCTNAGG 1 cut(s) 2985
BaeGI GKGCMC 1 cut(s) 3957
BamHI GGATCC 2 cut(s) 700, 2483
BanI GGYRCC 3 cut(s) 3954, 4354, 4476
BanII GRGCYC 2 cut(s) 256, 3900
BarI GAAGNNNNNNTAC 2 cut(s) 1257, 1289
BauI CACGAG 3 cut(s) 1317, 3542, 4455
Bbv12I GWGCWC 3 cut(s) 30, 3900, 4955
BbvCI CCTCAGC 1 cut(s) 4482
BbvI GCAGC 6 cut(s) 1668, 2209, 2642, 3238, 3396, 4657
BccI CCATC 9 cut(s) 68, 214, 1529, 2252, 2440, 2968, 3308, 3833, 4041
BceAI ACGGC 5 cut(s) 24, 89, 689, 3627, 4735
BcgI CGANNNNNNTGC 2 cut(s) 4436, 4470
BciT130I CCWGG 5 cut(s) 101, 969, 1514, 2418, 3099
BciVI GTATCC 1 cut(s) 1813
BclI TGATCA 9 cut(s) 405, 912, 999, 1389, 1843, 2845, 3169, 4957, 4983
BcnI CCSGG 1 cut(s) 1742
BcoDI GTCTC 8 cut(s) 1049, 1230, 2628, 2778, 3113, 3425, 3968, 4739
BcuI ACTAGT 1 cut(s) 2014
BfaI CTAG 9 cut(s) 1812, 2015, 2046, 3615, 3630, 4488, 4601, 5003, 5092
BfmI CTRYAG 6 cut(s) 246, 1873, 1975, 2474, 2767, 2778
BfrI CTTAAG 1 cut(s) 2330
BfuAI ACCTGC 1 cut(s) 2378
BfuI GTATCC 1 cut(s) 1813
BglII AGATCT 1 cut(s) 4851
BisI GCNGC 8 cut(s) 1606, 1657, 2223, 2631, 3252, 3385, 4671, 4995
BlnI CCTAGG 1 cut(s) 3629
BlpI GCTNAGC 1 cut(s) 414
BlsI GCNGC 8 cut(s) 1607, 1658, 2224, 2632, 3253, 3386, 4672, 4996
BmcAI AGTACT 1 cut(s) 188
Bme1390I CCNGG 6 cut(s) 101, 969, 1514, 1742, 2418, 3099
Bme18I GGWCC 3 cut(s) 333, 2279, 4509
BmeT110I CYCGRG 2 cut(s) 3464, 3719
BmgT120I GGNCC 4 cut(s) 333, 2279, 3842, 4509
BmiI GGNNCC 9 cut(s) 255, 702, 2485, 3061, 3598, 3956, 4026, 4356, 4478
BmrFI CCNGG 6 cut(s) 101, 969, 1514, 1742, 2418, 3099
BmrI ACTGGG 1 cut(s) 2431
BmuI ACTGGG 1 cut(s) 2431
BoxI GACNNNNGTC 1 cut(s) 2700
BplI GAGNNNNNCTC 2 cut(s) 57, 89
BpmI CTGGAG 8 cut(s) 240, 2694, 3695, 3948, 4075, 4162, 4309, 4899
Bpu10I CCTNAGC 1 cut(s) 4482
Bpu1102I GCTNAGC 1 cut(s) 414
Bpu14I TTCGAA 1 cut(s) 946
BpuEI CTTGAG 7 cut(s) 233, 1115, 2219, 2912, 3732, 4027, 5066
BpuMI CCSGG 1 cut(s) 1742
Bsa29I ATCGAT 1 cut(s) 4045
BsaBI GATNNNNATC 3 cut(s) 2103, 4041, 4527
BsaHI GRCGYC 3 cut(s) 17, 141, 2232
BsaI GGTCTC 1 cut(s) 2778
BsaWI WCCGGW 1 cut(s) 640
Bsc4I CCNNNNNNNGG 6 cut(s) 85, 2072, 2109, 2258, 2460, 4506
Bse118I RCCGGY 1 cut(s) 1159
Bse1I ACTGG 7 cut(s) 582, 1277, 1481, 2437, 2750, 4179, 4313
Bse21I CCTNAGG 1 cut(s) 2985
Bse3DI GCAATG 5 cut(s) 18, 263, 484, 960, 3323
Bse8I GATNNNNATC 3 cut(s) 2103, 4041, 4527
BseAI TCCGGA 1 cut(s) 640
BseBI CCWGG 5 cut(s) 101, 969, 1514, 2418, 3099
BseCI ATCGAT 1 cut(s) 4045
BseJI GATNNNNATC 3 cut(s) 2103, 4041, 4527
BseLI CCNNNNNNNGG 6 cut(s) 85, 2072, 2109, 2258, 2460, 4506
BseMI GCAATG 5 cut(s) 18, 263, 484, 960, 3323
BseMII CTCAG 9 cut(s) 428, 782, 2369, 2976, 3206, 4496, 4874, 4923, 4968
BseNI ACTGG 7 cut(s) 582, 1277, 1481, 2437, 2750, 4179, 4313
BseRI GAGGAG 4 cut(s) 901, 2567, 3872, 5087
BseSI GKGCMC 1 cut(s) 3957
BseXI GCAGC 6 cut(s) 1668, 2209, 2642, 3238, 3396, 4657
BseYI CCCAGC 1 cut(s) 3950
BsgI GTGCAG 1 cut(s) 1675
Bsh1236I CGCG 2 cut(s) 234, 4401
BshFI GGCC 6 cut(s) 104, 2428, 3401, 3611, 3844, 4722
BshNI GGYRCC 3 cut(s) 3954, 4354, 4476
BshVI ATCGAT 1 cut(s) 4045
BsiHKAI GWGCWC 3 cut(s) 30, 3900, 4955
BsiHKCI CYCGRG 2 cut(s) 3464, 3719
BsiSI CCGG 4 cut(s) 120, 641, 1160, 1741
BslFI GGGAC 5 cut(s) 2048, 2219, 2708, 3367, 3447
BslI CCNNNNNNNGG 6 cut(s) 85, 2072, 2109, 2258, 2460, 4506
BsmAI GTCTC 8 cut(s) 1049, 1230, 2628, 2778, 3113, 3425, 3968, 4739
BsmBI CGTCTC 3 cut(s) 1049, 3425, 3968
BsmFI GGGAC 5 cut(s) 2048, 2219, 2708, 3367, 3447
BsmI GAATGC 7 cut(s) 175, 833, 1688, 2302, 3135, 3450, 3452
BsnI GGCC 6 cut(s) 104, 2428, 3401, 3611, 3844, 4722
Bso31I GGTCTC 1 cut(s) 2778
BsoBI CYCGRG 2 cut(s) 3464, 3719
Bsp119I TTCGAA 1 cut(s) 946
Bsp1286I GDGCHC 5 cut(s) 30, 256, 3900, 3957, 4955
Bsp13I TCCGGA 1 cut(s) 640
Bsp1407I TGTACA 2 cut(s) 3201, 4564
Bsp1720I GCTNAGC 1 cut(s) 414
Bsp19I CCATGG 1 cut(s) 4680
BspANI GGCC 6 cut(s) 104, 2428, 3401, 3611, 3844, 4722
BspCNI CTCAG 9 cut(s) 427, 783, 2368, 2977, 3207, 4495, 4873, 4924, 4969
BspDI ATCGAT 1 cut(s) 4045
BspEI TCCGGA 1 cut(s) 640
BspFNI CGCG 2 cut(s) 234, 4401
BspHI TCATGA 2 cut(s) 3172, 3732
BspLI GGNNCC 9 cut(s) 255, 702, 2485, 3061, 3598, 3956, 4026, 4356, 4478
BspMAI CTGCAG 1 cut(s) 2478
BspMI ACCTGC 1 cut(s) 2378
BspQI GCTCTTC 3 cut(s) 453, 943, 3557
BspT104I TTCGAA 1 cut(s) 946
BspT107I GGYRCC 3 cut(s) 3954, 4354, 4476
BspTI CTTAAG 1 cut(s) 2330
BspTNI GGTCTC 1 cut(s) 2778
BsrBI CCGCTC 1 cut(s) 1915
BsrDI GCAATG 5 cut(s) 18, 263, 484, 960, 3323
BsrFI RCCGGY 1 cut(s) 1159
BsrGI TGTACA 2 cut(s) 3201, 4564
BsrI ACTGG 7 cut(s) 582, 1277, 1481, 2437, 2750, 4179, 4313
BssAI RCCGGY 1 cut(s) 1159
BssNI GRCGYC 3 cut(s) 17, 141, 2232
BssSI CACGAG 3 cut(s) 1317, 3542, 4455
Bst2BI CACGAG 3 cut(s) 1317, 3542, 4455
Bst2UI CCWGG 5 cut(s) 101, 969, 1514, 2418, 3099
Bst6I CTCTTC 7 cut(s) 453, 943, 2044, 3240, 3557, 4569, 4883
BstACI GRCGYC 3 cut(s) 17, 141, 2232
BstAFI CTTAAG 1 cut(s) 2330
BstAPI GCANNNNNTGC 1 cut(s) 1692
BstAUI TGTACA 2 cut(s) 3201, 4564
BstBI TTCGAA 1 cut(s) 946
BstC8I GCNNGC 6 cut(s) 125, 739, 1341, 1807, 1839, 2170
BstDSI CCRYGG 1 cut(s) 4680
BstFNI CGCG 2 cut(s) 234, 4401
BstHHI GCGC 4 cut(s) 1643, 1811, 4401, 4707
BstMAI GTCTC 8 cut(s) 1049, 1230, 2628, 2778, 3113, 3425, 3968, 4739
BstNI CCWGG 5 cut(s) 101, 969, 1514, 2418, 3099
BstNSI RCATGY 5 cut(s) 844, 1553, 1809, 2598, 4092
BstPAI GACNNNNGTC 1 cut(s) 2700
BstSCI CCNGG 6 cut(s) 99, 967, 1512, 1740, 2416, 3097
BstSFI CTRYAG 6 cut(s) 246, 1873, 1975, 2474, 2767, 2778
BstSLI GKGCMC 1 cut(s) 3957
BstUI CGCG 2 cut(s) 234, 4401
BstV1I GCAGC 6 cut(s) 1668, 2209, 2642, 3238, 3396, 4657
BstX2I RGATCY 7 cut(s) 91, 700, 2483, 2783, 4050, 4370, 4851
BstXI CCANNNNNNTGG 4 cut(s) 268, 577, 3607, 4687
BstYI RGATCY 7 cut(s) 91, 700, 2483, 2783, 4050, 4370, 4851
Bsu15I ATCGAT 1 cut(s) 4045
Bsu36I CCTNAGG 1 cut(s) 2985
BsuI GTATCC 1 cut(s) 1813
BsuRI GGCC 6 cut(s) 104, 2428, 3401, 3611, 3844, 4722
BsuTUI ATCGAT 1 cut(s) 4045
BtgI CCRYGG 1 cut(s) 4680
BtgZI GCGATG 3 cut(s) 948, 4396, 4416
BtsI GCAGTG 2 cut(s) 328, 408
BveI ACCTGC 1 cut(s) 2378
Cac8I GCNNGC 6 cut(s) 125, 739, 1341, 1807, 1839, 2170
CaiI CAGNNNCTG 2 cut(s) 4034, 4373
CciI TCATGA 2 cut(s) 3172, 3732
CfoI GCGC 4 cut(s) 1643, 1811, 4401, 4707
Cfr10I RCCGGY 1 cut(s) 1159
Cfr13I GGNCC 4 cut(s) 333, 2279, 3842, 4509
ClaI ATCGAT 1 cut(s) 4045
CseI GACGC 1 cut(s) 25
Eam1104I CTCTTC 7 cut(s) 453, 943, 2044, 3240, 3557, 4569, 4883
EarI CTCTTC 7 cut(s) 453, 943, 2044, 3240, 3557, 4569, 4883
EciI GGCGGA 1 cut(s) 1043
Ecl136II GAGCTC 1 cut(s) 3898
Eco24I GRGCYC 2 cut(s) 256, 3900
Eco31I GGTCTC 1 cut(s) 2778
Eco47I GGWCC 3 cut(s) 333, 2279, 4509
Eco53kI GAGCTC 1 cut(s) 3898
Eco57I CTGAAG 7 cut(s) 825, 2771, 2972, 3092, 3368, 3581, 4941
Eco81I CCTNAGG 1 cut(s) 2985
Eco88I CYCGRG 2 cut(s) 3464, 3719
EcoICRI GAGCTC 1 cut(s) 3898
EcoRI GAATTC 6 cut(s) 872, 1900, 2152, 2600, 3176, 4312
EcoRII CCWGG 5 cut(s) 99, 967, 1512, 2416, 3097
EcoT22I ATGCAT 5 cut(s) 982, 1511, 3289, 3452, 4090
EcoT38I GRGCYC 2 cut(s) 256, 3900
Esp3I CGTCTC 3 cut(s) 1049, 3425, 3968
FalI AAGNNNNNCTT 2 cut(s) 1077, 1109
FaqI GGGAC 5 cut(s) 2048, 2219, 2708, 3367, 3447
FauI CCCGC 3 cut(s) 239, 861, 2080
FauNDI CATATG 2 cut(s) 1933, 4267
FbaI TGATCA 9 cut(s) 405, 912, 999, 1389, 1843, 2845, 3169, 4957, 4983
FblI GTMKAC 1 cut(s) 1872
Fnu4HI GCNGC 8 cut(s) 1606, 1657, 2223, 2631, 3252, 3385, 4671, 4995
FriOI GRGCYC 2 cut(s) 256, 3900
Fsp4HI GCNGC 8 cut(s) 1606, 1657, 2223, 2631, 3252, 3385, 4671, 4995
FspBI CTAG 9 cut(s) 1812, 2015, 2046, 3615, 3630, 4488, 4601, 5003, 5092
FspI TGCGCA 1 cut(s) 1642
GlaI GCGC 4 cut(s) 1642, 1810, 4400, 4706
GluI GCNGC 8 cut(s) 1606, 1657, 2223, 2631, 3252, 3385, 4671, 4995
GsaI CCCAGC 1 cut(s) 3954
GsuI CTGGAG 8 cut(s) 240, 2694, 3695, 3948, 4075, 4162, 4309, 4899
HaeIII GGCC 6 cut(s) 104, 2428, 3401, 3611, 3844, 4722
HapII CCGG 4 cut(s) 120, 641, 1160, 1741
HgaI GACGC 1 cut(s) 25
HhaI GCGC 4 cut(s) 1643, 1811, 4401, 4707
Hin1I GRCGYC 3 cut(s) 17, 141, 2232
Hin6I GCGC 4 cut(s) 1641, 1809, 4399, 4705
HinP1I GCGC 4 cut(s) 1641, 1809, 4399, 4705
HincII GTYRAC 4 cut(s) 2347, 2497, 4131, 4717
HindII GTYRAC 4 cut(s) 2347, 2497, 4131, 4717
HindIII AAGCTT 6 cut(s) 593, 1394, 1919, 2192, 3294, 3981
HpaI GTTAAC 1 cut(s) 2347
HpaII CCGG 4 cut(s) 120, 641, 1160, 1741
Hpy99I CGWCG 1 cut(s) 10
HpyCH4IV ACGT 8 cut(s) 141, 854, 940, 1823, 2232, 4296, 4596, 4787
HpySE526I ACGT 8 cut(s) 141, 854, 940, 1823, 2232, 4296, 4596, 4787
Hsp92I GRCGYC 3 cut(s) 17, 141, 2232
HspAI GCGC 4 cut(s) 1641, 1809, 4399, 4705
Kpn2I TCCGGA 1 cut(s) 640
KpnI GGTACC 2 cut(s) 4358, 4480
Ksp22I TGATCA 9 cut(s) 405, 912, 999, 1389, 1843, 2845, 3169, 4957, 4983
KspAI GTTAAC 1 cut(s) 2347
LguI GCTCTTC 3 cut(s) 453, 943, 3557
Lsp1109I GCAGC 6 cut(s) 1668, 2209, 2642, 3238, 3396, 4657
MaeI CTAG 9 cut(s) 1812, 2015, 2046, 3615, 3630, 4488, 4601, 5003, 5092
MaeII ACGT 8 cut(s) 141, 854, 940, 1823, 2232, 4296, 4596, 4787
MbiI CCGCTC 1 cut(s) 1915
MfeI CAATTG 3 cut(s) 726, 1585, 4427
MflI RGATCY 7 cut(s) 91, 700, 2483, 2783, 4050, 4370, 4851
MhlI GDGCHC 5 cut(s) 30, 256, 3900, 3957, 4955
MmeI TCCRAC 1 cut(s) 307
Mph1103I ATGCAT 5 cut(s) 982, 1511, 3289, 3452, 4090
MroI TCCGGA 1 cut(s) 640
MroXI GAANNNNTTC 4 cut(s) 162, 942, 2615, 3450
MslI CAYNNNNRTG 4 cut(s) 995, 1613, 3011, 4685
MspA1I CMGCKG 3 cut(s) 803, 2714, 3387
MspCI CTTAAG 1 cut(s) 2330
MspI CCGG 4 cut(s) 120, 641, 1160, 1741
MspR9I CCNGG 6 cut(s) 101, 969, 1514, 1742, 2418, 3099
MunI CAATTG 3 cut(s) 726, 1585, 4427
Mva1269I GAATGC 7 cut(s) 175, 833, 1688, 2302, 3135, 3450, 3452
MvaI CCWGG 5 cut(s) 101, 969, 1514, 2418, 3099
MvnI CGCG 2 cut(s) 234, 4401
NciI CCSGG 1 cut(s) 1742
NcoI CCATGG 1 cut(s) 4680
NdeI CATATG 2 cut(s) 1933, 4267
NlaIV GGNNCC 9 cut(s) 255, 702, 2485, 3061, 3598, 3956, 4026, 4356, 4478
NmeAIII GCCGAG 1 cut(s) 45
NmuCI GTSAC 5 cut(s) 1756, 2955, 3521, 3704, 4732
NsbI TGCGCA 1 cut(s) 1642
NsiI ATGCAT 5 cut(s) 982, 1511, 3289, 3452, 4090
NspI RCATGY 5 cut(s) 844, 1553, 1809, 2598, 4092
NspV TTCGAA 1 cut(s) 946
PaeI GCATGC 1 cut(s) 1809
PagI TCATGA 2 cut(s) 3172, 3732
PciI ACATGT 2 cut(s) 840, 1549
PciSI GCTCTTC 3 cut(s) 453, 943, 3557
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PctI GAATGC 7 cut(s) 175, 833, 1688, 2302, 3135, 3450, 3452
PdmI GAANNNNTTC 4 cut(s) 162, 942, 2615, 3450
PflMI CCANNNNNTGG 2 cut(s) 2109, 2258
PkrI GCNGC 8 cut(s) 1607, 1658, 2224, 2632, 3253, 3386, 4672, 4996
PscI ACATGT 2 cut(s) 840, 1549
PshAI GACNNNNGTC 1 cut(s) 2700
PshBI ATTAAT 1 cut(s) 771
PsiI TTATAA 2 cut(s) 2210, 2939
Psp124BI GAGCTC 1 cut(s) 3900
Psp1406I AACGTT 1 cut(s) 854
Psp6I CCWGG 5 cut(s) 99, 967, 1512, 2416, 3097
PspFI CCCAGC 1 cut(s) 3950
PspGI CCWGG 5 cut(s) 99, 967, 1512, 2416, 3097
PspN4I GGNNCC 9 cut(s) 255, 702, 2485, 3061, 3598, 3956, 4026, 4356, 4478
PspPI GGNCC 4 cut(s) 333, 2279, 3842, 4509
PstI CTGCAG 1 cut(s) 2478
PstNI CAGNNNCTG 2 cut(s) 4034, 4373
PsuI RGATCY 7 cut(s) 91, 700, 2483, 2783, 4050, 4370, 4851
PvuII CAGCTG 3 cut(s) 803, 2714, 3387
RseI CAYNNNNRTG 4 cut(s) 995, 1613, 3011, 4685
SacI GAGCTC 1 cut(s) 3900
SapI GCTCTTC 3 cut(s) 453, 943, 3557
SatI GCNGC 8 cut(s) 1606, 1657, 2223, 2631, 3252, 3385, 4671, 4995
Sau96I GGNCC 4 cut(s) 333, 2279, 3842, 4509
ScaI AGTACT 1 cut(s) 188
ScrFI CCNGG 6 cut(s) 101, 969, 1514, 1742, 2418, 3099
SduI GDGCHC 5 cut(s) 30, 256, 3900, 3957, 4955
SfcI CTRYAG 6 cut(s) 246, 1873, 1975, 2474, 2767, 2778
SfuI TTCGAA 1 cut(s) 946
SinI GGWCC 3 cut(s) 333, 2279, 4509
SmiMI CAYNNNNRTG 4 cut(s) 995, 1613, 3011, 4685
SmlI CTYRAG 8 cut(s) 212, 1094, 2198, 2330, 2891, 3747, 4006, 5081
SmoI CTYRAG 8 cut(s) 212, 1094, 2198, 2330, 2891, 3747, 4006, 5081
SpeI ACTAGT 1 cut(s) 2014
SphI GCATGC 1 cut(s) 1809
SspI AATATT 1 cut(s) 2833
SspMI CTAG 9 cut(s) 1812, 2015, 2046, 3615, 3630, 4488, 4601, 5003, 5092
SstI GAGCTC 1 cut(s) 3900
StyD4I CCNGG 6 cut(s) 99, 967, 1512, 1740, 2416, 3097
TaiI ACGT 8 cut(s) 144, 857, 943, 1826, 2235, 4299, 4599, 4790
TaqI TCGA 6 cut(s) 662, 946, 1434, 1700, 4045, 4443
TatI WGTACW 5 cut(s) 186, 1554, 3201, 3546, 4564
TauI GCSGC 2 cut(s) 1608, 4997
TseFI GTSAC 5 cut(s) 1756, 2955, 3521, 3704, 4732
TseI GCWGC 6 cut(s) 1656, 2222, 2630, 3251, 3384, 4670
Tsp45I GTSAC 5 cut(s) 1756, 2955, 3521, 3704, 4732
TspGWI ACGGA 4 cut(s) 58, 101, 2476, 3992
Van91I CCANNNNNTGG 2 cut(s) 2109, 2258
Vha464I CTTAAG 1 cut(s) 2330
VpaK11BI GGWCC 3 cut(s) 333, 2279, 4509
VspI ATTAAT 1 cut(s) 771
XceI RCATGY 5 cut(s) 844, 1553, 1809, 2598, 4092
XmaJI CCTAGG 1 cut(s) 3629
XmiI GTMKAC 1 cut(s) 1872
XmnI GAANNNNTTC 4 cut(s) 162, 942, 2615, 3450
XspI CTAG 9 cut(s) 1812, 2015, 2046, 3615, 3630, 4488, 4601, 5003, 5092
ZraI GACGTC 2 cut(s) 142, 2233
ZrmI AGTACT 1 cut(s) 188
Zsp2I ATGCAT 5 cut(s) 982, 1511, 3289, 3452, 4090
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.