Rh5BG528500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000526
Physical Location & Seq
Forward (+)
83220449 .. 83222138
1690 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG528500.1

Sequence Viewer

Length: 312 bp
ATGGAGATTAGGAACGCTGAAGAGAATGAATACGAAGAAGGTGTGGTGCCAGAGATGAAATTTGTGTTGACCAAGGAAGACGTAACAGAGACTGGAGCACCAGCTACTCTGTTGGTTTTCAATAATGAAGTTGGCTTTTCCAGGGAGAACATGGATTCCATTTGCGGCATATTCCATTCTACAAAGATGGAAAAGAGACAGCAGAGGTTAATTGAAGAGAATGGTACCGGCCTCTACAGCATTGCTATTTCTGGTCTCAATGATGCCTTGTGGGAAAGAATTCATTGCCTGTTCACTCAAGAGAGTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

103

Amino Acids

11.79

Weight (kDa)

4.63

Isoelectric Point (pI)

53.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28020 AT3G48770 AT3G48770
fragaria_vesca FvH4_5g09020 FvH4_5g09020
malus_domestica MD06G1092400.v1.1 MD06G1134600.v1.1 MD06G1134700.v1.1 MD06G1134800.v1.1 MD06G1135200.v1.1 MD06G1135400.v1.1 MD06G1135500.v1.1 MD08G1137600.v1.1 MD15G1395100.v1.1
prunus_persica Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145600_v2.0.a1 Prupe.5G145800_v2.0.a1 Prupe.5G145900_v2.0.a1
pyrus_communis pycom06g12550 pycom06g12570 pycom06g12580 pycom06g12590 pycom06g12610 pycom06g12630 pycom06g12640 pycom06g12650 pycom08g06280
rosa_chinensis RchiOBHm_Chr6g0249321 RchiOBHm_Chr6g0275111 RchiOBHm_Chr7g0189191 RchiOBHm_Chr7g0189261
rosa_laevigata RLG00000004622 RLG00000004623 RLG00000008237 RLG00000008238 RLG00000023303 RLG00000035187
rosa_multiflora Rmu_co8294311.1_g000001 Rmu_co8389833.1_g000001 Rmu_sc0001044.1_g000004 Rmu_sc0001329.1_g000011 Rmu_sc0006012.1_g000010 Rmu_sc0007286.1_g000032 Rmu_sc0009360.1_g000016 Rmu_sc0011817.1_g000001 Rmu_sc0015050.1_g000001
rosa_roxburghii Rroxscaffold_1G00064000 Rroxscaffold_3G00265460 Rroxscaffold_3G00265580 Rroxscaffold_3G00265640 Rroxscaffold_3G00265690 Rroxscaffold_3G00265700 Rroxscaffold_7G00193620 Rroxscaffold_7G00204550 Rroxscaffold_7G00217350
rosa_rugosa Rorug01G0465500 Rorug06G0088500 Rorug06G0496700 Rorug06G0496700
rosa_samantha Rh1AG064800 Rh2BG015100 Rh2BG493500 Rh2DG017300 Rh2DG502500 Rh4BG208500 Rh5BG528500 Rh5CG552900 Rh6CG207800 Rh6DG197900 Rh6DG198000 Rh7AG101300 Rh7AG101400 Rh7AG101600 Rh7BG104100 Rh7BG104200 Rh7CG105600 Rh7CG105700 Rh7DG103200 Rh7DG103400
rosa_wichuraiana Rw1G007300 Rw5G034670 Rw7G008750 Rw7G008780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 224
AccB1I GGYRCC 2 cut(s) 46, 224
AciI CCGC 1 cut(s) 165
AcsI RAATTY 2 cut(s) 59, 279
AcuI CTGAAG 1 cut(s) 39
AfaI GTAC 1 cut(s) 226
AgsI TTSAA 2 cut(s) 121, 215
AjnI CCWGG 1 cut(s) 140
AloI GAACNNNNNNTCC 2 cut(s) 140, 172
AluBI AGCT 1 cut(s) 104
AluI AGCT 1 cut(s) 104
Alw21I GWGCWC 1 cut(s) 100
Alw26I GTCTC 3 cut(s) 83, 190, 260
AlwNI CAGNNNCTG 1 cut(s) 92
AoxI GGCC 1 cut(s) 229
ApoI RAATTY 2 cut(s) 59, 279
Asp700I GAANNNNTTC 1 cut(s) 279
Asp718I GGTACC 1 cut(s) 224
BanI GGYRCC 2 cut(s) 46, 224
BbsI GAAGAC 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 100
BccI CCATC 1 cut(s) 181
BciT130I CCWGG 1 cut(s) 142
BcoDI GTCTC 3 cut(s) 83, 190, 260
BfmI CTRYAG 1 cut(s) 235
BisI GCNGC 1 cut(s) 166
BlsI GCNGC 1 cut(s) 167
Bme1390I CCNGG 1 cut(s) 142
BmiI GGNNCC 2 cut(s) 48, 226
BmrFI CCNGG 1 cut(s) 142
BmsI GCATC 1 cut(s) 253
BpiI GAAGAC 1 cut(s) 84
BpmI CTGGAG 1 cut(s) 114
BpuEI CTTGAG 1 cut(s) 282
BsaI GGTCTC 1 cut(s) 260
BsaJI CCNNGG 2 cut(s) 72, 141
Bse118I RCCGGY 1 cut(s) 227
Bse1I ACTGG 1 cut(s) 97
Bse3DI GCAATG 2 cut(s) 240, 283
BseBI CCWGG 1 cut(s) 142
BseDI CCNNGG 2 cut(s) 72, 141
BseMI GCAATG 2 cut(s) 240, 283
BseNI ACTGG 1 cut(s) 97
BshFI GGCC 1 cut(s) 231
BshNI GGYRCC 2 cut(s) 46, 224
BsiHKAI GWGCWC 1 cut(s) 100
BsiSI CCGG 1 cut(s) 228
BsmAI GTCTC 3 cut(s) 83, 190, 260
BsnI GGCC 1 cut(s) 231
Bso31I GGTCTC 1 cut(s) 260
Bsp1286I GDGCHC 1 cut(s) 100
BspACI CCGC 1 cut(s) 165
BspANI GGCC 1 cut(s) 231
BspLI GGNNCC 2 cut(s) 48, 226
BspT107I GGYRCC 2 cut(s) 46, 224
BspTNI GGTCTC 1 cut(s) 260
BsrDI GCAATG 2 cut(s) 240, 283
BsrFI RCCGGY 1 cut(s) 227
BsrI ACTGG 1 cut(s) 97
BssAI RCCGGY 1 cut(s) 227
BssECI CCNNGG 2 cut(s) 72, 141
BssT1I CCWWGG 1 cut(s) 72
Bst2UI CCWGG 1 cut(s) 142
Bst6I CTCTTC 2 cut(s) 15, 210
BstMAI GTCTC 3 cut(s) 83, 190, 260
BstMWI GCNNNNNNNGC 1 cut(s) 237
BstNI CCWGG 1 cut(s) 142
BstSCI CCNGG 1 cut(s) 140
BstSFI CTRYAG 1 cut(s) 235
BstV2I GAAGAC 1 cut(s) 84
BsuRI GGCC 1 cut(s) 231
CaiI CAGNNNCTG 1 cut(s) 92
Cfr10I RCCGGY 1 cut(s) 227
Csp6I GTAC 1 cut(s) 225
CviAII CATG 1 cut(s) 151
CviJI RGCY 3 cut(s) 104, 135, 231
CviKI_1 RGCY 3 cut(s) 104, 135, 231
CviQI GTAC 1 cut(s) 225
Eam1104I CTCTTC 2 cut(s) 15, 210
EarI CTCTTC 2 cut(s) 15, 210
Eco130I CCWWGG 1 cut(s) 72
Eco31I GGTCTC 1 cut(s) 260
Eco57I CTGAAG 1 cut(s) 39
EcoRI GAATTC 1 cut(s) 279
EcoRII CCWGG 1 cut(s) 140
EcoT14I CCWWGG 1 cut(s) 72
ErhI CCWWGG 1 cut(s) 72
FaeI CATG 1 cut(s) 154
FaiI YATR 2 cut(s) 152, 170
FatI CATG 1 cut(s) 150
Fnu4HI GCNGC 1 cut(s) 166
Fsp4HI GCNGC 1 cut(s) 166
GluI GCNGC 1 cut(s) 166
GsuI CTGGAG 1 cut(s) 114
HaeIII GGCC 1 cut(s) 231
HapII CCGG 1 cut(s) 228
Hin1II CATG 1 cut(s) 154
HincII GTYRAC 1 cut(s) 69
HindII GTYRAC 1 cut(s) 69
HinfI GANTC 1 cut(s) 155
HpaII CCGG 1 cut(s) 228
Hpy166II GTNNAC 2 cut(s) 69, 294
Hpy188III TCNNGA 1 cut(s) 299
Hpy8I GTNNAC 2 cut(s) 69, 294
HpyAV CCTTC 1 cut(s) 32
HpyCH4IV ACGT 1 cut(s) 81
HpyF10VI GCNNNNNNNGC 1 cut(s) 237
HpySE526I ACGT 1 cut(s) 81
Hsp92II CATG 1 cut(s) 154
KpnI GGTACC 1 cut(s) 228
LmnI GCTCC 1 cut(s) 95
LpnPI CCDG 8 cut(s) 63, 78, 114, 127, 154, 237, 241, 302
LweI GCATC 1 cut(s) 253
MaeII ACGT 1 cut(s) 81
MaeIII GTNAC 1 cut(s) 82
MboII GAAGA 4 cut(s) 32, 47, 89, 227
MhlI GDGCHC 1 cut(s) 100
MluCI AATT 3 cut(s) 59, 210, 279
MnlI CCTC 2 cut(s) 198, 242
MroXI GAANNNNTTC 1 cut(s) 279
MseI TTAA 1 cut(s) 209
MspI CCGG 1 cut(s) 228
MspR9I CCNGG 1 cut(s) 142
MvaI CCWGG 1 cut(s) 142
MwoI GCNNNNNNNGC 1 cut(s) 237
NlaIII CATG 1 cut(s) 154
NlaIV GGNNCC 2 cut(s) 48, 226
PdmI GAANNNNTTC 1 cut(s) 279
PfeI GAWTC 1 cut(s) 155
PkrI GCNGC 1 cut(s) 167
Psp6I CCWGG 1 cut(s) 140
PspGI CCWGG 1 cut(s) 140
PspN4I GGNNCC 2 cut(s) 48, 226
PstNI CAGNNNCTG 1 cut(s) 92
RsaI GTAC 1 cut(s) 226
RsaNI GTAC 1 cut(s) 225
SaqAI TTAA 1 cut(s) 209
SatI GCNGC 1 cut(s) 166
ScrFI CCNGG 1 cut(s) 142
SduI GDGCHC 1 cut(s) 100
SetI ASST 4 cut(s) 43, 84, 106, 209
SfaNI GCATC 1 cut(s) 253
SfcI CTRYAG 1 cut(s) 235
SmlI CTYRAG 1 cut(s) 297
SmoI CTYRAG 1 cut(s) 297
Sse9I AATT 3 cut(s) 59, 210, 279
SsiI CCGC 1 cut(s) 165
StyD4I CCNGG 1 cut(s) 140
StyI CCWWGG 1 cut(s) 72
TaiI ACGT 1 cut(s) 84
TasI AATT 3 cut(s) 59, 210, 279
TauI GCSGC 1 cut(s) 168
TfiI GAWTC 1 cut(s) 155
Tru1I TTAA 1 cut(s) 209
Tru9I TTAA 1 cut(s) 209
TspDTI ATGAA 4 cut(s) 42, 71, 141, 272
XapI RAATTY 2 cut(s) 59, 279
XcmI CCANNNNNNNNNTGG 1 cut(s) 148
XmnI GAANNNNTTC 1 cut(s) 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.