pycom06g12650

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0000526
Physical Location & Seq
Forward (+)
17766940 .. 17770074
3135 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g12650.1

Sequence Viewer

Length: 3135 bp
ATGTATACAAATTTGCCTTTCATAATTCAGGCAGATTTTCTGTTACAATCATCGAGAGAAGGTATTGTCTTGGACAGCAAGTGGAATCAAGGAATTCTTAGTTGTGTGCCTGTTGCATTCCTCAAAGCATTTATCTCGCTCGTGAAAACTATAGGAGACGCACCAGATTCTAGTTTGCCTCCTTACTTTAGGTTCTTACCTGTCCAGAACACCTCCTATGATGAATTGAATGTTGTGAGGGAGTCAATCAAAGCACTGCTTATTGAGGAAGACATTGTACCAACCAAGTCACACAAGCAGAGGTTCTTCCATAAACCTCGTGAAGTGGGTAGGTTGTTACCTGCTTTCTGGAATATTTTGAGAAAGGCGAGCCATGAAAGAGTTAGCTTGGGGAACATCTCTTCCCATGGAAGATATGTTTTGTGTGATTCATTTGACAAAGAGGAGTATGATCACATTCTGAATTTCCTTGAAGTTAAACCAGTTGACGATGAGTGGTATGCTAAATGCATTCAGAGTTCCGATATTGTAGCCGGAGTGTCTGAAGATGTCTATTGGGAGCTTCTGTTGTTTGTTTCCGATAACTGGAGGCGGACATTCCACTGCACCAACATCAAGAACATTCCACTGATAAAATATGTGGATTTTGACGGAAGTGTAGCATTCTGCAGCTTAAGCGCAATGCGGATTCGTGAAAAGAAGATTTGCCTATCCCGTCTTCCTAGTCATGTCTCATGGTTGATTGATTGGAACAAGGAATTTATATCCACAGCAAGTCTTCTTTTTATTCCAGAAATAACCCAGCAAGCTCTCCAGTCATGTTCTAAGAAGGAGGAGTTGCTGAAATGGTTCGCGGAAGAAATGAAGGTTGCTGCTGTAAGTGTTTACGAGTATGCAGTTTGCCTCGATCATTCTCTTGGCGATGAGCGGAAACCTGTTGTTGCATATGCCCACTTTTTGTATCATTCATTGTGCAATAAGTATATCTCTGCTTACGAAGTTGATGATTTATGTCGTAAAATGCCACTTGTGAATAACTACGGCTACGTTAATAGACAAAGGAGAGGGGTTATTGTCCCTGCCAATGAAAGCAAATGGGCTGATTTGACTGATACAAATCTGTGGACGGAAGAAGGCTACATCGAGTTAGGAGAAGATTACATAAAACCAGGCCGCTTTGCAGGTAAAGTTACAAAAGAGAAGCAGCTCCTTGAGTTCCTCAAAGATCACGCATTTGCAGCTTTGGATGTCCCTTATATTTCTGCTCCCAATGCATGTATTCCAACTGTGTCTTCAACTCTTACGAAGCAAAATGCATTCTTGCTTTTGGACTGGATTCATCACTTGAAATACGAGAGGGTTCACATTCCAGAGAAGTTTTTGAAAAGCATAGCGAATGGTTACTGGCTGAAAGTTTATTTGAATGGATATTCGGGTGCCAGCAGGCCACCTTCGCAGTCATTCATGCTCAAGTCATCATGTGGAAACATTCTGCAGAGTGGATCTAATTTTGTTGATATCCCATTGGTTGACATGAGTTATTATGGCGAGAAAATTAATGATTACAAGGAGGAGCTAAAAGCAATTGGAGTCATGTTTGAGTATGCAGAAGCATGCAAGTTTATTGGGAACCGTCTTATGTCTCTCGCAGATTCATCCACTTTAACTATAGACAGTGTACTTTCAATCCTGAAATTTATCAAGTTTTTGAGGGACAACTATTTTTCAGCCGAAGAATTTGTATCCAGTATTAAAAGGGGGAGATGGCTTAAAACGTCCCATGGCTACAGGTCTCCAGTTGGATCTGTCTTGTTCAACCAAGAGTGGCGAATTGCATCCAGGATTAGTGACATTCCCTTCATTGAGCAGGAGTTTTACGGTGAAGAAATTCTTCATTTCAAAACAGAACTCGAGTTGCTTGGTGTGGTTGGTTCCTTGAACGGAAATTACCAGCTCATCATCGACCACCTAAAGTCATTGTCATACTTAACTCATCTAACATCGGATGCTTTTCTGCTGTTGCTGCAAATTATGAAGACTTCAAATTCATCTGATAAGATTGTCCAAGCATTGAGTGGAGCAAGATGCCTGAAAACAAACAATGGTTACAAGTTTCCACATGAATGTCTCTTGTTTCACGAGGAATGGGGTTGCCTTCTTCAGGTGTTTAGTGGTCTCCCATTGGTTGATCACAATTTCTATGGAGACAACATTTTTTCCTACAGGAATGAGTTGAAGAAAATAGGGGTGGTTGTTGATTATGAGGAGGCTGCAAAAGAATTTGCTGGTTATTTCAAGCAGCATGCATCTTGGGCTTCCATCACCAAGGAAAATGTTGCATCATTTCTGTTGTGCTGTAGAAAGCTGAAGGGAACTCCTTTTAAATTTCCCACAGATCTGAAGAGCTGCATTCGTGAAGAAAAGTGGTTGCGAACTAGTCGTGGTGATTATAGATCTCCAAGAGAGTGCATTCTCTTTAGTCCTGATTGGGAATCTATCTCTCCAATATCTCGTCTCCCTTTTATCGACGATTCTGAAAATTATTATGGCAAGAATATTCATGAATATAAGAAGGAGCTCAAGAGCATGGGGGTCGCTGTGGAATTCAAGGATGGTGTCAAGTTTGTTCCTCCTAATGTTTGTCTTCCCCAGAATCAAAGCAGCATTTCCCCAGAAAATGCGCTAGCATTGCTGGAATGCATGCATATTTTGTTGGAGGTGAAAGATTACTCCTTTTCAGATGCATTTATAAAGAGAGTTTCTCAACCATGGTTGAAGACTTATGCTGGTTATAGGCCTCCGAGCGAGTGCTTGTTGTTCGACTCCAAATTTGATTTGTTTCTGAAAAAGACTGACGGGCCTTTCATTGACGAAGAATTTTATGGTTCTAAAATTACAACCTACAGAAAAGAGCTCTCAGAAATAGGGGTGATCGTTGAAGTGGAACAAGGATGTCCATTGATTGCCAGCCACCTTGATTTTCATGATGAACGTTCTACTTTCGTTCGAGTATATGAGTACTTGAATGAGTTTAAGTGGAAGCCGGATTGCGAGGCTGACAAAAGGATCGATCATCAACATGGATTCCGAATGGAAATCAGAATGGAGCGTGGGCTAGTCCTGATCAATGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1045

Amino Acids

120.25

Weight (kDa)

6.21

Isoelectric Point (pI)

47.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28020 AT3G48770 AT3G48770
fragaria_vesca FvH4_5g09020 FvH4_5g09020
malus_domestica MD06G1092400.v1.1 MD06G1134600.v1.1 MD06G1134700.v1.1 MD06G1134800.v1.1 MD06G1135200.v1.1 MD06G1135400.v1.1 MD06G1135500.v1.1 MD08G1137600.v1.1 MD15G1395100.v1.1
prunus_persica Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145600_v2.0.a1 Prupe.5G145800_v2.0.a1 Prupe.5G145900_v2.0.a1
pyrus_communis pycom06g12550 pycom06g12570 pycom06g12580 pycom06g12590 pycom06g12610 pycom06g12630 pycom06g12640 pycom06g12650 pycom08g06280
rosa_chinensis RchiOBHm_Chr6g0249321 RchiOBHm_Chr6g0275111 RchiOBHm_Chr7g0189191 RchiOBHm_Chr7g0189261
rosa_laevigata RLG00000004622 RLG00000004623 RLG00000008237 RLG00000008238 RLG00000023303 RLG00000035187
rosa_multiflora Rmu_co8294311.1_g000001 Rmu_co8389833.1_g000001 Rmu_sc0001044.1_g000004 Rmu_sc0001329.1_g000011 Rmu_sc0006012.1_g000010 Rmu_sc0007286.1_g000032 Rmu_sc0009360.1_g000016 Rmu_sc0011817.1_g000001 Rmu_sc0015050.1_g000001
rosa_roxburghii Rroxscaffold_1G00064000 Rroxscaffold_3G00265460 Rroxscaffold_3G00265580 Rroxscaffold_3G00265640 Rroxscaffold_3G00265690 Rroxscaffold_3G00265700 Rroxscaffold_7G00193620 Rroxscaffold_7G00204550 Rroxscaffold_7G00217350
rosa_rugosa Rorug01G0465500 Rorug06G0088500 Rorug06G0496700 Rorug06G0496700
rosa_samantha Rh1AG064800 Rh2BG015100 Rh2BG493500 Rh2DG017300 Rh2DG502500 Rh4BG208500 Rh5BG528500 Rh5CG552900 Rh6CG207800 Rh6DG197900 Rh6DG198000 Rh7AG101300 Rh7AG101400 Rh7AG101600 Rh7BG104100 Rh7BG104200 Rh7CG105600 Rh7CG105700 Rh7DG103200 Rh7DG103400
rosa_wichuraiana Rw1G007300 Rw5G034670 Rw7G008750 Rw7G008780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2750
Acc36I ACCTGC 2 cut(s) 349, 1172
AccB1I GGYRCC 1 cut(s) 1436
AccBSI CCGCTC 1 cut(s) 928
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 854
AciI CCGC 5 cut(s) 592, 685, 854, 928, 1174
AclI AACGTT 1 cut(s) 2992
AclWI GGATC 3 cut(s) 1510, 1810, 3074
AcuI CTGAAG 4 cut(s) 564, 2144, 2387, 2420
AfaI GTAC 3 cut(s) 279, 1680, 3020
AfiI CCNNNNNNNGG 2 cut(s) 585, 2161
AflII CTTAAG 1 cut(s) 673
AhlI ACTAGT 1 cut(s) 2435
AjnI CCWGG 2 cut(s) 1168, 1838
AjuI GAANNNNNNNTTGG 2 cut(s) 1276, 1308
AloI GAACNNNNNNTCC 2 cut(s) 386, 418
Alw21I GWGCWC 2 cut(s) 2580, 2916
Alw26I GTCTC 8 cut(s) 150, 736, 1647, 1797, 2132, 2180, 2199, 2519
AlwI GGATC 3 cut(s) 1510, 1810, 3074
Ama87I CYCGRG 1 cut(s) 1910
AoxI GGCC 4 cut(s) 1171, 1445, 2795, 2858
ApeKI GCWGC 9 cut(s) 669, 872, 1204, 1238, 2023, 2270, 2299, 2406, 2661
ArsI GACNNNNNNTTYG 2 cut(s) 2813, 2845
AseI ATTAAT 1 cut(s) 1557
Asp700I GAANNNNTTC 3 cut(s) 848, 1887, 1890
AspLEI GCGC 2 cut(s) 680, 2683
AspS9I GGNCC 1 cut(s) 2858
AsuHPI GGTGA 5 cut(s) 1892, 2314, 2456, 2731, 2941
AsuNHI GCTAGC 1 cut(s) 2683
AvaI CYCGRG 1 cut(s) 1910
BanI GGYRCC 1 cut(s) 1436
BanII GRGCYC 2 cut(s) 2580, 2916
BarI GAAGNNNNNNTAC 2 cut(s) 261, 293
BauI CACGAG 3 cut(s) 140, 318, 2138
BbsI GAAGAC 7 cut(s) 276, 710, 770, 1284, 2042, 2636, 2783
Bbv12I GWGCWC 2 cut(s) 2580, 2916
BbvI GCAGC 9 cut(s) 681, 859, 1216, 1250, 2010, 2257, 2311, 2393, 2673
BccI CCATC 3 cut(s) 1758, 2327, 2606
BceAI ACGGC 1 cut(s) 1057
BcgI CGANNNNNNTGC 4 cut(s) 117, 151, 2575, 2609
BciT130I CCWGG 2 cut(s) 1170, 1840
BciVI GTATCC 1 cut(s) 1753
BclI TGATCA 3 cut(s) 451, 2188, 3123
BcoDI GTCTC 8 cut(s) 150, 736, 1647, 1797, 2132, 2180, 2199, 2519
BcuI ACTAGT 1 cut(s) 2435
BfaI CTAG 5 cut(s) 171, 723, 2436, 2684, 3116
BfmI CTRYAG 8 cut(s) 150, 667, 1493, 1668, 1786, 2221, 2356, 2902
BfrI CTTAAG 1 cut(s) 673
BfuAI ACCTGC 2 cut(s) 349, 1172
BfuI GTATCC 1 cut(s) 1753
BglII AGATCT 2 cut(s) 2395, 2453
BmcAI AGTACT 1 cut(s) 3020
Bme1390I CCNGG 2 cut(s) 1170, 1840
BmeT110I CYCGRG 1 cut(s) 1910
BmgT120I GGNCC 1 cut(s) 2858
BmiI GGNNCC 3 cut(s) 1438, 1631, 1933
BmrFI CCNGG 2 cut(s) 1170, 1840
BmsI GCATC 6 cut(s) 1844, 1996, 2075, 2315, 2348, 2731
BmtI GCTAGC 1 cut(s) 2687
BpiI GAAGAC 7 cut(s) 276, 710, 770, 1284, 2042, 2636, 2783
BplI GAGNNNNNCTC 2 cut(s) 2746, 2778
BpmI CTGGAG 3 cut(s) 607, 797, 1779
BpuEI CTTGAG 3 cut(s) 1232, 1454, 2564
Bsa29I ATCGAT 1 cut(s) 3069
BsaBI GATNNNNATC 1 cut(s) 1116
BsaI GGTCTC 2 cut(s) 1797, 2180
BsaJI CCNNGG 4 cut(s) 406, 1780, 2325, 2768
BsaXI ACNNNNNCTCC 2 cut(s) 551, 581
Bsc4I CCNNNNNNNGG 2 cut(s) 585, 2161
Bse1I ACTGG 7 cut(s) 482, 590, 814, 1337, 1409, 1746, 1796
Bse3DI GCAATG 2 cut(s) 687, 2687
Bse8I GATNNNNATC 1 cut(s) 1116
BseBI CCWGG 2 cut(s) 1170, 1840
BseCI ATCGAT 1 cut(s) 3069
BseDI CCNNGG 4 cut(s) 406, 1780, 2325, 2768
BseGI GGATG 6 cut(s) 1252, 1655, 1835, 2011, 2617, 2957
BseJI GATNNNNATC 1 cut(s) 1116
BseLI CCNNNNNNNGG 2 cut(s) 585, 2161
BseMI GCAATG 2 cut(s) 687, 2687
BseMII CTCAG 1 cut(s) 2931
BseNI ACTGG 7 cut(s) 482, 590, 814, 1337, 1409, 1746, 1796
BseRI GAGGAG 4 cut(s) 458, 848, 1586, 2279
BseXI GCAGC 9 cut(s) 681, 859, 1216, 1250, 2010, 2257, 2311, 2393, 2673
BseYI CCCAGC 1 cut(s) 801
BsgI GTGCAG 1 cut(s) 589
Bsh1236I CGCG 1 cut(s) 854
BshFI GGCC 4 cut(s) 1173, 1447, 2797, 2860
BshNI GGYRCC 1 cut(s) 1436
BshVI ATCGAT 1 cut(s) 3069
BsiHKAI GWGCWC 2 cut(s) 2580, 2916
BsiHKCI CYCGRG 1 cut(s) 1910
BsiSI CCGG 2 cut(s) 534, 3044
BslFI GGGAC 4 cut(s) 1061, 1235, 1727, 1762
BslI CCNNNNNNNGG 2 cut(s) 585, 2161
BsmAI GTCTC 8 cut(s) 150, 736, 1647, 1797, 2132, 2180, 2199, 2519
BsmBI CGTCTC 2 cut(s) 150, 2519
BsmFI GGGAC 4 cut(s) 1061, 1235, 1727, 1762
BsmI GAATGC 7 cut(s) 116, 510, 662, 1316, 2409, 2469, 2702
BsnI GGCC 4 cut(s) 1173, 1447, 2797, 2860
Bso31I GGTCTC 2 cut(s) 1797, 2180
BsoBI CYCGRG 1 cut(s) 1910
Bsp1286I GDGCHC 2 cut(s) 2580, 2916
Bsp19I CCATGG 3 cut(s) 406, 1780, 2768
BspACI CCGC 5 cut(s) 592, 685, 854, 928, 1174
BspANI GGCC 4 cut(s) 1173, 1447, 2797, 2860
BspCNI CTCAG 1 cut(s) 2930
BspDI ATCGAT 1 cut(s) 3069
BspFNI CGCG 1 cut(s) 854
BspHI TCATGA 2 cut(s) 2560, 2983
BspLI GGNNCC 3 cut(s) 1438, 1631, 1933
BspMAI CTGCAG 2 cut(s) 671, 1497
BspMI ACCTGC 2 cut(s) 349, 1172
BspOI GCTAGC 1 cut(s) 2687
BspPI GGATC 3 cut(s) 1510, 1810, 3074
BspQI GCTCTTC 1 cut(s) 2396
BspT107I GGYRCC 1 cut(s) 1436
BspTI CTTAAG 1 cut(s) 673
BspTNI GGTCTC 2 cut(s) 1797, 2180
BsrBI CCGCTC 1 cut(s) 928
BsrDI GCAATG 2 cut(s) 687, 2687
BsrI ACTGG 7 cut(s) 482, 590, 814, 1337, 1409, 1746, 1796
BssECI CCNNGG 4 cut(s) 406, 1780, 2325, 2768
BssNAI GTATAC 1 cut(s) 6
BssSI CACGAG 3 cut(s) 140, 318, 2138
BssT1I CCWWGG 4 cut(s) 406, 1780, 2325, 2768
Bst1107I GTATAC 1 cut(s) 6
Bst2BI CACGAG 3 cut(s) 140, 318, 2138
Bst2UI CCWGG 2 cut(s) 1170, 1840
Bst4CI ACNGT 4 cut(s) 1288, 1634, 1676, 1880
Bst6I CTCTTC 2 cut(s) 406, 2396
BstAFI CTTAAG 1 cut(s) 673
BstC8I GCNNGC 9 cut(s) 370, 807, 1441, 1445, 1615, 2304, 2685, 2702, 2968
BstDEI CTNAG 3 cut(s) 98, 825, 2917
BstDSI CCRYGG 3 cut(s) 406, 1780, 2768
BstENI CCTNNNNNAGG 1 cut(s) 2159
BstF5I GGATG 6 cut(s) 1252, 1655, 1835, 2011, 2617, 2957
BstFNI CGCG 1 cut(s) 854
BstHHI GCGC 2 cut(s) 680, 2683
BstMAI GTCTC 8 cut(s) 150, 736, 1647, 1797, 2132, 2180, 2199, 2519
BstMWI GCNNNNNNNGC 7 cut(s) 675, 1238, 1271, 1453, 2023, 2312, 2689
BstNI CCWGG 2 cut(s) 1170, 1840
BstNSI RCATGY 4 cut(s) 1278, 1617, 2306, 2704
BstSCI CCNGG 2 cut(s) 1168, 1838
BstSFI CTRYAG 8 cut(s) 150, 667, 1493, 1668, 1786, 2221, 2356, 2902
BstUI CGCG 1 cut(s) 854
BstV1I GCAGC 9 cut(s) 681, 859, 1216, 1250, 2010, 2257, 2311, 2393, 2673
BstV2I GAAGAC 7 cut(s) 276, 710, 770, 1284, 2042, 2636, 2783
BstX2I RGATCY 4 cut(s) 1502, 1802, 2395, 2453
BstYI RGATCY 4 cut(s) 1502, 1802, 2395, 2453
BstZ17I GTATAC 1 cut(s) 6
Bsu15I ATCGAT 1 cut(s) 3069
BsuI GTATCC 1 cut(s) 1753
BsuRI GGCC 4 cut(s) 1173, 1447, 2797, 2860
BsuTUI ATCGAT 1 cut(s) 3069
BtgI CCRYGG 3 cut(s) 406, 1780, 2768
BtgZI GCGATG 1 cut(s) 936
BtsCI GGATG 6 cut(s) 1252, 1655, 1835, 2011, 2617, 2957
BtsI GCAGTG 2 cut(s) 254, 601
BtsIMutI CAGTG 4 cut(s) 254, 601, 626, 1681
BveI ACCTGC 2 cut(s) 349, 1172
Cac8I GCNNGC 9 cut(s) 370, 807, 1441, 1445, 1615, 2304, 2685, 2702, 2968
CciI TCATGA 2 cut(s) 2560, 2983
CfoI GCGC 2 cut(s) 680, 2683
Cfr13I GGNCC 1 cut(s) 2858
ClaI ATCGAT 1 cut(s) 3069
CseI GACGC 1 cut(s) 167
Csp6I GTAC 3 cut(s) 278, 1679, 3019
CviQI GTAC 3 cut(s) 278, 1679, 3019
DdeI CTNAG 3 cut(s) 98, 825, 2917
DraI TTTAAA 1 cut(s) 2383
Eam1104I CTCTTC 2 cut(s) 406, 2396
EarI CTCTTC 2 cut(s) 406, 2396
EciI GGCGGA 1 cut(s) 607
Ecl136II GAGCTC 2 cut(s) 2578, 2914
Eco130I CCWWGG 4 cut(s) 406, 1780, 2325, 2768
Eco147I AGGCCT 1 cut(s) 2797
Eco24I GRGCYC 2 cut(s) 2580, 2916
Eco31I GGTCTC 2 cut(s) 1797, 2180
Eco32I GATATC 1 cut(s) 1519
Eco53kI GAGCTC 2 cut(s) 2578, 2914
Eco57I CTGAAG 4 cut(s) 564, 2144, 2387, 2420
Eco88I CYCGRG 1 cut(s) 1910
EcoICRI GAGCTC 2 cut(s) 2578, 2914
EcoNI CCTNNNNNAGG 1 cut(s) 2159
EcoRI GAATTC 2 cut(s) 93, 2603
EcoRII CCWGG 2 cut(s) 1168, 1838
EcoRV GATATC 1 cut(s) 1519
EcoT14I CCWWGG 4 cut(s) 406, 1780, 2325, 2768
EcoT22I ATGCAT 7 cut(s) 512, 1276, 1318, 2308, 2702, 2706, 2746
EcoT38I GRGCYC 2 cut(s) 2580, 2916
ErhI CCWWGG 4 cut(s) 406, 1780, 2325, 2768
Esp3I CGTCTC 2 cut(s) 150, 2519
FalI AAGNNNNNCTT 6 cut(s) 81, 113, 243, 275, 1875, 1907
FaqI GGGAC 4 cut(s) 1061, 1235, 1727, 1762
FauNDI CATATG 1 cut(s) 946
FbaI TGATCA 3 cut(s) 451, 2188, 3123
FblI GTMKAC 1 cut(s) 5
FokI GGATG 6 cut(s) 1259, 1642, 1822, 2018, 2624, 2964
FriOI GRGCYC 2 cut(s) 2580, 2916
FspBI CTAG 5 cut(s) 171, 723, 2436, 2684, 3116
GlaI GCGC 2 cut(s) 679, 2682
GsaI CCCAGC 1 cut(s) 805
GsuI CTGGAG 3 cut(s) 607, 797, 1779
HaeIII GGCC 4 cut(s) 1173, 1447, 2797, 2860
HapII CCGG 2 cut(s) 534, 3044
HgaI GACGC 1 cut(s) 167
HhaI GCGC 2 cut(s) 680, 2683
Hin6I GCGC 2 cut(s) 678, 2681
HinP1I GCGC 2 cut(s) 678, 2681
HincII GTYRAC 2 cut(s) 487, 1531
HindII GTYRAC 2 cut(s) 487, 1531
HpaII CCGG 2 cut(s) 534, 3044
HphI GGTGA 5 cut(s) 1892, 2314, 2456, 2731, 2941
Hpy166II GTNNAC 7 cut(s) 6, 487, 886, 1125, 1363, 1531, 1679
Hpy8I GTNNAC 7 cut(s) 6, 487, 886, 1125, 1363, 1531, 1679
Hpy99I CGWCG 1 cut(s) 2531
HpyAV CCTTC 9 cut(s) 53, 823, 859, 1127, 1461, 1867, 2165, 2362, 2566
HpyCH4III ACNGT 4 cut(s) 1288, 1634, 1676, 1880
HpyCH4IV ACGT 3 cut(s) 1047, 1775, 2992
HpyF10VI GCNNNNNNNGC 7 cut(s) 675, 1238, 1271, 1453, 2023, 2312, 2689
HpyF3I CTNAG 3 cut(s) 98, 825, 2917
HpySE526I ACGT 3 cut(s) 1047, 1775, 2992
HspAI GCGC 2 cut(s) 678, 2681
Ksp22I TGATCA 3 cut(s) 451, 2188, 3123
LguI GCTCTTC 1 cut(s) 2396
LmnI GCTCC 7 cut(s) 559, 1212, 1270, 1573, 2078, 2575, 3106
Lsp1109I GCAGC 9 cut(s) 681, 859, 1216, 1250, 2010, 2257, 2311, 2393, 2673
LweI GCATC 6 cut(s) 1844, 1996, 2075, 2315, 2348, 2731
MaeI CTAG 5 cut(s) 171, 723, 2436, 2684, 3116
MaeII ACGT 3 cut(s) 1047, 1775, 2992
MaeIII GTNAC 7 cut(s) 42, 288, 336, 1189, 1400, 1847, 2105
MbiI CCGCTC 1 cut(s) 928
MfeI CAATTG 1 cut(s) 1584
MflI RGATCY 4 cut(s) 1502, 1802, 2395, 2453
MhlI GDGCHC 2 cut(s) 2580, 2916
MlyI GAGTC 3 cut(s) 251, 1599, 2816
MmeI TCCRAC 3 cut(s) 1307, 1780, 2694
Mph1103I ATGCAT 7 cut(s) 512, 1276, 1318, 2308, 2702, 2706, 2746
MroXI GAANNNNTTC 3 cut(s) 848, 1887, 1890
MslI CAYNNNNRTG 2 cut(s) 2122, 3078
MspCI CTTAAG 1 cut(s) 673
MspI CCGG 2 cut(s) 534, 3044
MspR9I CCNGG 2 cut(s) 1170, 1840
MunI CAATTG 1 cut(s) 1584
Mva1269I GAATGC 7 cut(s) 116, 510, 662, 1316, 2409, 2469, 2702
MvaI CCWGG 2 cut(s) 1170, 1840
MvnI CGCG 1 cut(s) 854
MwoI GCNNNNNNNGC 7 cut(s) 675, 1238, 1271, 1453, 2023, 2312, 2689
NcoI CCATGG 3 cut(s) 406, 1780, 2768
NdeI CATATG 1 cut(s) 946
NheI GCTAGC 1 cut(s) 2683
NlaIV GGNNCC 3 cut(s) 1438, 1631, 1933
NmuCI GTSAC 2 cut(s) 288, 1847
NsiI ATGCAT 7 cut(s) 512, 1276, 1318, 2308, 2702, 2706, 2746
NspI RCATGY 4 cut(s) 1278, 1617, 2306, 2704
PaeI GCATGC 3 cut(s) 1617, 2306, 2704
PaeR7I CTCGAG 1 cut(s) 1910
PagI TCATGA 2 cut(s) 2560, 2983
PceI AGGCCT 1 cut(s) 2797
PciSI GCTCTTC 1 cut(s) 2396
PctI GAATGC 7 cut(s) 116, 510, 662, 1316, 2409, 2469, 2702
PdmI GAANNNNTTC 3 cut(s) 848, 1887, 1890
PfoI TCCNGGA 1 cut(s) 1838
PleI GAGTC 3 cut(s) 250, 1598, 2816
PpsI GAGTC 3 cut(s) 250, 1598, 2816
PshBI ATTAAT 1 cut(s) 1557
PsiI TTATAA 1 cut(s) 2750
Psp124BI GAGCTC 2 cut(s) 2580, 2916
Psp1406I AACGTT 1 cut(s) 2992
Psp6I CCWGG 2 cut(s) 1168, 1838
PspFI CCCAGC 1 cut(s) 801
PspGI CCWGG 2 cut(s) 1168, 1838
PspN4I GGNNCC 3 cut(s) 1438, 1631, 1933
PspPI GGNCC 1 cut(s) 2858
PspXI VCTCGAGB 1 cut(s) 1910
PsrI GAACNNNNNNTAC 2 cut(s) 1931, 1963
PstI CTGCAG 2 cut(s) 671, 1497
PsuI RGATCY 4 cut(s) 1502, 1802, 2395, 2453
RsaI GTAC 3 cut(s) 279, 1680, 3020
RsaNI GTAC 3 cut(s) 278, 1679, 3019
RseI CAYNNNNRTG 2 cut(s) 2122, 3078
SacI GAGCTC 2 cut(s) 2580, 2916
SapI GCTCTTC 1 cut(s) 2396
Sau96I GGNCC 1 cut(s) 2858
ScaI AGTACT 1 cut(s) 3020
SchI GAGTC 3 cut(s) 251, 1599, 2816
ScrFI CCNGG 2 cut(s) 1170, 1840
SduI GDGCHC 2 cut(s) 2580, 2916
SfaNI GCATC 6 cut(s) 1844, 1996, 2075, 2315, 2348, 2731
SfcI CTRYAG 8 cut(s) 150, 667, 1493, 1668, 1786, 2221, 2356, 2902
Sfr274I CTCGAG 1 cut(s) 1910
SlaI CTCGAG 1 cut(s) 1910
SmiMI CAYNNNNRTG 2 cut(s) 2122, 3078
SmlI CTYRAG 5 cut(s) 673, 1211, 1469, 1910, 2579
SmoI CTYRAG 5 cut(s) 673, 1211, 1469, 1910, 2579
SpeI ACTAGT 1 cut(s) 2435
SphI GCATGC 3 cut(s) 1617, 2306, 2704
SseBI AGGCCT 1 cut(s) 2797
SsiI CCGC 5 cut(s) 592, 685, 854, 928, 1174
SspI AATATT 2 cut(s) 355, 2557
SspMI CTAG 5 cut(s) 171, 723, 2436, 2684, 3116
SstI GAGCTC 2 cut(s) 2580, 2916
StuI AGGCCT 1 cut(s) 2797
StyD4I CCNGG 2 cut(s) 1168, 1838
StyI CCWWGG 4 cut(s) 406, 1780, 2325, 2768
TaaI ACNGT 4 cut(s) 1288, 1634, 1676, 1880
TaiI ACGT 3 cut(s) 1050, 1778, 2995
TaqI TCGA 9 cut(s) 53, 906, 1143, 1911, 1962, 2526, 2820, 3007, 3069
TatI WGTACW 2 cut(s) 1678, 3018
TauI GCSGC 1 cut(s) 1176
TscAI CASTG 4 cut(s) 261, 608, 633, 1681
TseFI GTSAC 2 cut(s) 288, 1847
TseI GCWGC 9 cut(s) 669, 872, 1204, 1238, 2023, 2270, 2299, 2406, 2661
Tsp45I GTSAC 2 cut(s) 288, 1847
TspGWI ACGGA 3 cut(s) 666, 1142, 1956
TspRI CASTG 4 cut(s) 261, 608, 633, 1681
Vha464I CTTAAG 1 cut(s) 673
VspI ATTAAT 1 cut(s) 1557
XagI CCTNNNNNAGG 1 cut(s) 2159
XceI RCATGY 4 cut(s) 1278, 1617, 2306, 2704
XcmI CCANNNNNNNNNTGG 1 cut(s) 2072
XhoI CTCGAG 1 cut(s) 1910
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 3 cut(s) 848, 1887, 1890
XspI CTAG 5 cut(s) 171, 723, 2436, 2684, 3116
ZrmI AGTACT 1 cut(s) 3020
Zsp2I ATGCAT 7 cut(s) 512, 1276, 1318, 2308, 2702, 2706, 2746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.