MD06G1135500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000526
Physical Location & Seq
Forward (+)
28050981 .. 28054845
3865 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1135500.v1.1.491

Sequence Viewer

Length: 2880 bp
ATGGTTCACAGTGTACTACTTATTTTGCAGAATGCAGAAGATAATGAGTACCCAGAAGGGGTTAATCCATCACTTGAGTTTGTCATAACTTCCCGGGATATAACAGCCGTGGGGGCGTTCCACCATTTGCAGTGTTGGGCGTTCCCCCAAGAAAGGCAACAGGAACTGCGGCTACATTGGGGAGAAAGGTATGTGTATATACACGAGATGCTTCTATATATTCTGCATGCCAGTCCTTTTTATTACGAGTTTATGACCACTAAGTATCAATATTTCCATCTTGAAATGTCATTTGTTTCAAAGAATTATGTACTCTTATCGGAGTCATGTAATAGTAATACTGCTTTGATAATGGCGGACAAGGTTGAAGCCGTTAAGCAACAACTCTCTAGCATGCATCCGGAAATTCTCTTGTTCTTATCAAGGATTAAGCGACTCTCGGTTAGGGAAGACAATGTGGATCCAAGGCACAACACTGTAAGTGCGATAGCAATATCAAGTGAGACTAATTTTGTCACCAGGAAGAACATAGATGCTGAATCCTACACACTCCACCTTTCTGCGAAAGAAAATGGCTACAAATTTGATGCAGAATGCGGCTATTATATGTGGAAGCAGAAGTTTCCTGTCAGGCGGGAGTGCAGAGTAGATAAGATAAATGATGTAGATGAATGGATGATCACACTTGCATTTCCAATTGGAGAGCACTTTTGTAGAGGAACAAACACATCTCCTGGAGTCTATGCATTCCTTCCCACCGAGATGGTGACAAACTTTCCCTTTATAATTGAAGCCGATTTTCTTCTTGCCTCATCAAGGGAAACAATTCTTCTGGACAGCAAATGGAATAAGGGGATTCTCGATTGCATTCCAACTGCATTTTTGAATGCATTTATCTCGCTTGTCAGATCTAAAGAAGATGCTCCAGTTTCTAGCTTGACTCGTATGGTCAAGTTTCTGCCCGTTCAGAGCTCTTATGAAGGATTGAATGTTGTCAGCGATGGTAGCTGGCAGAAAGTTACTTTGAATGGTTCTCCTGGTTTCAGACCACCATCGCAGTCATTTCTTCTCAAGTCATCATCGGGAAACCTTTTGCAGAATAGCTCTGTTTTTGTTGATATACCGTTGATTGATCAAAGTCATTATGATGGAAAGATTAACGATTACAAGGAGGAGCTGAAAAAAATTGGAGTCAGGTTTGAGTTTGGAGAAGCGTGTGAATATATGGGGAAGCATCTCATGTCTCTTGCTGCTGCTTCCTCTTTAACCCGTGGCAATGTATTTTCTGTACTACGTTTCATCAGATTTTTGAGAGAGAAACTTCTTCCACCAGACGATTTTATCAGCAGTATTAAAGATGGTCAGTGGCTTAAAACTTCCCTTGGCTTCAGGTCTCTGGTTGGATCTGTCCTGTCGGATGATGAGTGGACAGTTGCTTCGCAAATTAGTGACATTCCCTTCATAGATGAGGCATTCTATGGTAAAGAAGAAATCTGTCAGTTCAAAACAGAACTTGAGTTGCTTGGGGTGGCGGTCTCATTCAGCAAATGCTACCAACTGATAATTGACAACCTCAAGTCACATTCTTGCTTGACTTCCTGGACACCTGAGGTTCTTCTATTGATGCTTAAATGCATGTGCCTTTCGAGCTCATTATTTCAAGCAGCATGCATGCATCTTCGACTTCCATTTCAAAAGAAAATGTTGCATCAATTCTCGTGTTACAGAACACTGAATGTAACTCCATTTAAATTTCCTGCAGATTTTAAGAGCTGCATTCGCGAGGTGAAGTGGCTGCGTTCTCGCCTTGGTGATTATAGATCTCCCAAACAGTGCATTCTCTCTGGTCCCGAGGGAAAACTTTCTCGAGCATGGTTGAAGACTCATGTTGGTTACCATTCTCCTGACCAGTGCTTGTTGTTTGATTCGAAATCGCGTTTAAAGCAAACTGATGGGCCTTTCATTGATGAAGAATTCTATGGTTCTAAAATTACAACCTACAGAAAGGAGCTCGCTGCAATTGGGGTGATTGTCGAAGTGAAAAAGGGAGGTGCCTTGATTGCAAGGCACCTTGATCTACATGATGAGTTTTCGACATTTGTTCGAGTATATAGTTACTTGAGTGACTCTAAGTGGGTGCCACTGGCAGATGGAGAGGCTCCTGGAAGGATCTGGGTTCTGAAAGAAAATAAAAGTTGTTTGGTGGAATTTTGCTGTGCAACAAGGAGGACGTTTTCATTGCAGATGACCTTCATCTGCAGTATCTTTACAAACAGGTACCCTCAGCCAAACTTGGCTTCCTTGCCTCGGACTAGGGTGCTTGAAATCTACAAGAAAATTGGTGTTCGCACTATTTCAGAATCTGTGCAGAAGGTAGAATCATCCCTCGCAGATGATGTTGAACTACTGATGATTCCCACGAAGAAATTGATGGGGAAAACGCTCTTGAGGCTGATTCTTGGGTTTCTAGCTGGTCCTCCCATTGAGATGGAAGTAGAGAAGAGGCAGAAAGCTGTCCAAGGTCTTGTAGACATTGCTGTTGTGGAGACACCTGAACCAATCATTGTGAGCTATGATCTGCCACTATCTTCTGGTGAAGTTTTGAATGTGAAAGGCACACGAAAGATACGGTGGGACAGGGAGAATTCCAAATTCTTCATCCAGAAGATGGACAAATCTGGCGGACAGAAGAGTACCATTGAGTTTGCTACCTATTTTGCTGAAGCAATATCTGAGTGTGTGCTGTGGGAGTGCACTGAACATATACCTGCACTTTCTGAACTCATCAAGTTGGCATTTGTGCTAGAATTCAATGAGGAAGCTGTTGATTTCTTGATGAAGTTGAAGAATCTGCAGATCTTCGTTGAGGATGGGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

960

Amino Acids

109.49

Weight (kDa)

6.44

Isoelectric Point (pI)

47.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28020 AT3G48770 AT3G48770
fragaria_vesca FvH4_5g09020 FvH4_5g09020
malus_domestica MD06G1092400.v1.1 MD06G1134600.v1.1 MD06G1134700.v1.1 MD06G1134800.v1.1 MD06G1135200.v1.1 MD06G1135400.v1.1 MD06G1135500.v1.1 MD08G1137600.v1.1 MD15G1395100.v1.1
prunus_persica Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145600_v2.0.a1 Prupe.5G145800_v2.0.a1 Prupe.5G145900_v2.0.a1
pyrus_communis pycom06g12550 pycom06g12570 pycom06g12580 pycom06g12590 pycom06g12610 pycom06g12630 pycom06g12640 pycom06g12650 pycom08g06280
rosa_chinensis RchiOBHm_Chr6g0249321 RchiOBHm_Chr6g0275111 RchiOBHm_Chr7g0189191 RchiOBHm_Chr7g0189261
rosa_laevigata RLG00000004622 RLG00000004623 RLG00000008237 RLG00000008238 RLG00000023303 RLG00000035187
rosa_multiflora Rmu_co8294311.1_g000001 Rmu_co8389833.1_g000001 Rmu_sc0001044.1_g000004 Rmu_sc0001329.1_g000011 Rmu_sc0006012.1_g000010 Rmu_sc0007286.1_g000032 Rmu_sc0009360.1_g000016 Rmu_sc0011817.1_g000001 Rmu_sc0015050.1_g000001
rosa_roxburghii Rroxscaffold_1G00064000 Rroxscaffold_3G00265460 Rroxscaffold_3G00265580 Rroxscaffold_3G00265640 Rroxscaffold_3G00265690 Rroxscaffold_3G00265700 Rroxscaffold_7G00193620 Rroxscaffold_7G00204550 Rroxscaffold_7G00217350
rosa_rugosa Rorug01G0465500 Rorug06G0088500 Rorug06G0496700 Rorug06G0496700
rosa_samantha Rh1AG064800 Rh2BG015100 Rh2BG493500 Rh2DG017300 Rh2DG502500 Rh4BG208500 Rh5BG528500 Rh5CG552900 Rh6CG207800 Rh6DG197900 Rh6DG198000 Rh7AG101300 Rh7AG101400 Rh7AG101600 Rh7BG104100 Rh7BG104200 Rh7CG105600 Rh7CG105700 Rh7DG103200 Rh7DG103400
rosa_wichuraiana Rw1G007300 Rw5G034670 Rw7G008750 Rw7G008780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 785
Acc36I ACCTGC 1 cut(s) 2776
Acc65I GGTACC 1 cut(s) 2277
AccB1I GGYRCC 4 cut(s) 2051, 2067, 2137, 2277
AccB7I CCANNNNNTGG 1 cut(s) 2668
AccI GTMKAC 1 cut(s) 2529
AccII CGCG 2 cut(s) 1782, 1936
AccIII TCCGGA 1 cut(s) 400
AciI CCGC 6 cut(s) 169, 356, 597, 634, 1532, 2682
AclWI GGATC 4 cut(s) 455, 468, 1411, 2177
AcsI RAATTY 8 cut(s) 405, 581, 1751, 1973, 2207, 2644, 2651, 2807
AcuI CTGAAG 2 cut(s) 1372, 2742
AfaI GTAC 6 cut(s) 15, 50, 312, 1290, 2279, 2695
AfiI CCNNNNNNNGG 5 cut(s) 58, 153, 816, 2307, 2668
AjnI CCWGG 5 cut(s) 518, 733, 1036, 1598, 2161
AjuI GAANNNNNNNTTGG 2 cut(s) 2281, 2313
Alw21I GWGCWC 5 cut(s) 708, 974, 1652, 2013, 2756
Alw26I GTCTC 5 cut(s) 497, 1248, 1398, 1540, 2540
Alw44I GTGCAC 1 cut(s) 2752
AlwI GGATC 4 cut(s) 455, 468, 1411, 2177
AlwNI CAGNNNCTG 2 cut(s) 166, 2363
Ama87I CYCGRG 3 cut(s) 93, 1850, 1866
Aor13HI TCCGGA 1 cut(s) 400
AoxI GGCC 1 cut(s) 1955
ApaLI GTGCAC 1 cut(s) 2752
ApeKI GCWGC 6 cut(s) 1250, 1253, 1664, 1773, 1795, 2015
ApoI RAATTY 8 cut(s) 405, 581, 1751, 1973, 2207, 2644, 2651, 2807
ArsI GACNNNNNNTTYG 2 cut(s) 1420, 1452
Asp700I GAANNNNTTC 2 cut(s) 825, 1861
Asp718I GGTACC 1 cut(s) 2277
AspS9I GGNCC 3 cut(s) 1847, 1955, 2474
AsuC2I CCSGG 2 cut(s) 94, 95
AsuHPI GGTGA 6 cut(s) 508, 778, 1798, 1823, 2038, 2606
AsuII TTCGAA 1 cut(s) 1928
AvaI CYCGRG 3 cut(s) 93, 1850, 1866
AvaII GGWCC 2 cut(s) 1847, 2474
AxyI CCTNAGG 1 cut(s) 1608
BaeGI GKGCMC 1 cut(s) 2756
BamHI GGATCC 1 cut(s) 460
BanI GGYRCC 4 cut(s) 2051, 2067, 2137, 2277
BanII GRGCYC 3 cut(s) 974, 1652, 2013
BauI CACGAG 2 cut(s) 203, 1717
BbsI GAAGAC 2 cut(s) 456, 1886
Bbv12I GWGCWC 5 cut(s) 708, 974, 1652, 2013, 2756
BbvCI CCTCAGC 1 cut(s) 2283
BbvI GCAGC 6 cut(s) 1237, 1240, 1676, 1760, 1782, 2002
BceAI ACGGC 2 cut(s) 92, 356
BcgI CGANNNNNNTGC 2 cut(s) 879, 913
BciT130I CCWGG 5 cut(s) 520, 735, 1038, 1600, 2163
BclI TGATCA 2 cut(s) 678, 1132
BcnI CCSGG 2 cut(s) 94, 95
BcoDI GTCTC 5 cut(s) 497, 1248, 1398, 1540, 2540
BfaI CTAG 5 cut(s) 390, 933, 2313, 2468, 2804
BfmI CTRYAG 4 cut(s) 1758, 1999, 2257, 2852
BfuAI ACCTGC 1 cut(s) 2776
BglI GCCNNNNNGGC 1 cut(s) 113
BglII AGATCT 3 cut(s) 908, 1820, 2856
BisI GCNGC 8 cut(s) 170, 598, 1251, 1254, 1665, 1774, 1796, 2016
BlsI GCNGC 8 cut(s) 171, 599, 1252, 1255, 1666, 1775, 1797, 2017
Bme1390I CCNGG 7 cut(s) 94, 95, 520, 735, 1038, 1600, 2163
Bme18I GGWCC 2 cut(s) 1847, 2474
BmeT110I CYCGRG 3 cut(s) 93, 1850, 1866
BmgT120I GGNCC 3 cut(s) 1847, 1955, 2474
BmiI GGNNCC 7 cut(s) 462, 1849, 2053, 2069, 2139, 2160, 2279
BmrFI CCNGG 7 cut(s) 94, 95, 520, 735, 1038, 1600, 2163
BmsI GCATC 9 cut(s) 198, 406, 523, 577, 910, 1243, 1614, 1684, 1717
BpiI GAAGAC 2 cut(s) 456, 1886
BpmI CTGGAG 2 cut(s) 756, 909
Bpu10I CCTNAGC 1 cut(s) 2283
Bpu14I TTCGAA 1 cut(s) 1928
BpuEI CTTGAG 6 cut(s) 95, 1055, 1535, 1559, 2140, 2467
BpuMI CCSGG 2 cut(s) 94, 95
BsaI GGTCTC 2 cut(s) 1398, 1540
BsaJI CCNNGG 9 cut(s) 93, 108, 464, 1270, 1381, 1807, 1851, 2306, 2518
BsaWI WCCGGW 1 cut(s) 400
Bsc4I CCNNNNNNNGG 5 cut(s) 58, 153, 816, 2307, 2668
Bse1I ACTGG 4 cut(s) 231, 926, 1909, 2148
Bse21I CCTNAGG 1 cut(s) 1608
Bse3DI GCAATG 3 cut(s) 1282, 2237, 2532
BseAI TCCGGA 1 cut(s) 400
BseBI CCWGG 5 cut(s) 520, 735, 1038, 1600, 2163
BseDI CCNNGG 9 cut(s) 93, 108, 464, 1270, 1381, 1807, 1851, 2306, 2518
BseGI GGATG 6 cut(s) 397, 681, 1423, 2381, 2658, 2875
BseLI CCNNNNNNNGG 5 cut(s) 58, 153, 816, 2307, 2668
BseMI GCAATG 3 cut(s) 1282, 2237, 2532
BseMII CTCAG 3 cut(s) 1599, 2297, 2724
BseNI ACTGG 4 cut(s) 231, 926, 1909, 2148
BseRI GAGGAG 1 cut(s) 1187
BseSI GKGCMC 1 cut(s) 2756
BseXI GCAGC 6 cut(s) 1237, 1240, 1676, 1760, 1782, 2002
BsgI GTGCAG 3 cut(s) 661, 2387, 2754
Bsh1236I CGCG 2 cut(s) 1782, 1936
BshFI GGCC 1 cut(s) 1957
BshNI GGYRCC 4 cut(s) 2051, 2067, 2137, 2277
BsiHKAI GWGCWC 5 cut(s) 708, 974, 1652, 2013, 2756
BsiHKCI CYCGRG 3 cut(s) 93, 1850, 1866
BsiSI CCGG 2 cut(s) 94, 401
BslFI GGGAC 2 cut(s) 1833, 2648
BslI CCNNNNNNNGG 5 cut(s) 58, 153, 816, 2307, 2668
BsmAI GTCTC 5 cut(s) 497, 1248, 1398, 1540, 2540
BsmFI GGGAC 2 cut(s) 1833, 2648
BsmI GAATGC 8 cut(s) 37, 599, 746, 867, 892, 1472, 1776, 1836
BsnI GGCC 1 cut(s) 1957
Bso31I GGTCTC 2 cut(s) 1398, 1540
BsoBI CYCGRG 3 cut(s) 93, 1850, 1866
Bsp119I TTCGAA 1 cut(s) 1928
Bsp1286I GDGCHC 5 cut(s) 708, 974, 1652, 2013, 2756
Bsp13I TCCGGA 1 cut(s) 400
Bsp68I TCGCGA 1 cut(s) 1782
BspACI CCGC 6 cut(s) 169, 356, 597, 634, 1532, 2682
BspANI GGCC 1 cut(s) 1957
BspCNI CTCAG 3 cut(s) 1600, 2296, 2725
BspEI TCCGGA 1 cut(s) 400
BspFNI CGCG 2 cut(s) 1782, 1936
BspLI GGNNCC 7 cut(s) 462, 1849, 2053, 2069, 2139, 2160, 2279
BspMAI CTGCAG 3 cut(s) 1762, 2261, 2856
BspMI ACCTGC 1 cut(s) 2776
BspPI GGATC 4 cut(s) 455, 468, 1411, 2177
BspT104I TTCGAA 1 cut(s) 1928
BspT107I GGYRCC 4 cut(s) 2051, 2067, 2137, 2277
BspTNI GGTCTC 2 cut(s) 1398, 1540
BsrDI GCAATG 3 cut(s) 1282, 2237, 2532
BsrI ACTGG 4 cut(s) 231, 926, 1909, 2148
BssECI CCNNGG 9 cut(s) 93, 108, 464, 1270, 1381, 1807, 1851, 2306, 2518
BssSI CACGAG 2 cut(s) 203, 1717
BssT1I CCWWGG 4 cut(s) 464, 1381, 1807, 2518
Bst2BI CACGAG 2 cut(s) 203, 1717
Bst2UI CCWGG 5 cut(s) 520, 735, 1038, 1600, 2163
Bst4CI ACNGT 6 cut(s) 11, 478, 1125, 1432, 1833, 2631
Bst6I CTCTTC 2 cut(s) 2495, 2684
BstBI TTCGAA 1 cut(s) 1928
BstC8I GCNNGC 6 cut(s) 228, 395, 1010, 1669, 1673, 2013
BstDEI CTNAG 5 cut(s) 261, 1608, 2130, 2283, 2733
BstDSI CCRYGG 2 cut(s) 108, 1270
BstEII GGTNACC 1 cut(s) 1892
BstENI CCTNNNNNAGG 1 cut(s) 814
BstF5I GGATG 6 cut(s) 397, 681, 1423, 2381, 2658, 2875
BstFNI CGCG 2 cut(s) 1782, 1936
BstMAI GTCTC 5 cut(s) 497, 1248, 1398, 1540, 2540
BstMWI GCNNNNNNNGC 7 cut(s) 113, 1005, 1647, 1779, 1942, 2060, 2449
BstNI CCWGG 5 cut(s) 520, 735, 1038, 1600, 2163
BstNSI RCATGY 5 cut(s) 230, 397, 1639, 1671, 1675
BstPI GGTNACC 1 cut(s) 1892
BstSCI CCNGG 7 cut(s) 92, 93, 518, 733, 1036, 1598, 2161
BstSFI CTRYAG 4 cut(s) 1758, 1999, 2257, 2852
BstSLI GKGCMC 1 cut(s) 2756
BstUI CGCG 2 cut(s) 1782, 1936
BstV1I GCAGC 6 cut(s) 1237, 1240, 1676, 1760, 1782, 2002
BstV2I GAAGAC 2 cut(s) 456, 1886
BstX2I RGATCY 6 cut(s) 460, 908, 1403, 1820, 2169, 2856
BstXI CCANNNNNNTGG 2 cut(s) 763, 2488
BstYI RGATCY 6 cut(s) 460, 908, 1403, 1820, 2169, 2856
Bsu36I CCTNAGG 1 cut(s) 1608
BsuRI GGCC 1 cut(s) 1957
BtgI CCRYGG 2 cut(s) 108, 1270
BtgZI GCGATG 2 cut(s) 1014, 1038
BtsCI GGATG 6 cut(s) 397, 681, 1423, 2381, 2658, 2875
BtsI GCAGTG 1 cut(s) 137
BtsIMutI CAGTG 9 cut(s) 16, 137, 474, 1370, 1730, 1838, 1916, 2141, 2754
BtuMI TCGCGA 1 cut(s) 1782
BveI ACCTGC 1 cut(s) 2776
Cac8I GCNNGC 6 cut(s) 228, 395, 1010, 1669, 1673, 2013
CaiI CAGNNNCTG 2 cut(s) 166, 2363
Cfr13I GGNCC 3 cut(s) 1847, 1955, 2474
Cfr9I CCCGGG 1 cut(s) 93
Csp6I GTAC 6 cut(s) 14, 49, 311, 1289, 2278, 2694
CviQI GTAC 6 cut(s) 14, 49, 311, 1289, 2278, 2694
DdeI CTNAG 5 cut(s) 261, 1608, 2130, 2283, 2733
DraI TTTAAA 2 cut(s) 1750, 1941
Eam1104I CTCTTC 2 cut(s) 2495, 2684
EarI CTCTTC 2 cut(s) 2495, 2684
EciI GGCGGA 2 cut(s) 371, 2697
Ecl136II GAGCTC 3 cut(s) 972, 1650, 2011
Eco130I CCWWGG 4 cut(s) 464, 1381, 1807, 2518
Eco24I GRGCYC 3 cut(s) 974, 1652, 2013
Eco31I GGTCTC 2 cut(s) 1398, 1540
Eco47I GGWCC 2 cut(s) 1847, 2474
Eco53kI GAGCTC 3 cut(s) 972, 1650, 2011
Eco57I CTGAAG 2 cut(s) 1372, 2742
Eco81I CCTNAGG 1 cut(s) 1608
Eco88I CYCGRG 3 cut(s) 93, 1850, 1866
Eco91I GGTNACC 1 cut(s) 1892
EcoICRI GAGCTC 3 cut(s) 972, 1650, 2011
EcoNI CCTNNNNNAGG 1 cut(s) 814
EcoO65I GGTNACC 1 cut(s) 1892
EcoRI GAATTC 3 cut(s) 1973, 2644, 2807
EcoRII CCWGG 5 cut(s) 518, 733, 1036, 1598, 2161
EcoT14I CCWWGG 4 cut(s) 464, 1381, 1807, 2518
EcoT22I ATGCAT 6 cut(s) 399, 748, 892, 1637, 1673, 1677
EcoT38I GRGCYC 3 cut(s) 974, 1652, 2013
ErhI CCWWGG 4 cut(s) 464, 1381, 1807, 2518
FaqI GGGAC 2 cut(s) 1833, 2648
FauI CCCGC 1 cut(s) 627
FbaI TGATCA 2 cut(s) 678, 1132
FblI GTMKAC 1 cut(s) 2529
Fnu4HI GCNGC 8 cut(s) 170, 598, 1251, 1254, 1665, 1774, 1796, 2016
FokI GGATG 5 cut(s) 384, 688, 1430, 2368, 2645
FriOI GRGCYC 3 cut(s) 974, 1652, 2013
Fsp4HI GCNGC 8 cut(s) 170, 598, 1251, 1254, 1665, 1774, 1796, 2016
FspBI CTAG 5 cut(s) 390, 933, 2313, 2468, 2804
GluI GCNGC 8 cut(s) 170, 598, 1251, 1254, 1665, 1774, 1796, 2016
GsuI CTGGAG 2 cut(s) 756, 909
HaeIII GGCC 1 cut(s) 1957
HapII CCGG 2 cut(s) 94, 401
HpaII CCGG 2 cut(s) 94, 401
HphI GGTGA 6 cut(s) 508, 778, 1798, 1823, 2038, 2606
Hpy166II GTNNAC 5 cut(s) 7, 14, 1428, 2530, 2754
Hpy8I GTNNAC 5 cut(s) 7, 14, 1428, 2530, 2754
HpyAV CCTTC 7 cut(s) 50, 761, 974, 1468, 2160, 2260, 2365
HpyCH4III ACNGT 6 cut(s) 11, 478, 1125, 1432, 1833, 2631
HpyCH4IV ACGT 2 cut(s) 1294, 2231
HpyF10VI GCNNNNNNNGC 7 cut(s) 113, 1005, 1647, 1779, 1942, 2060, 2449
HpyF3I CTNAG 5 cut(s) 261, 1608, 2130, 2283, 2733
HpySE526I ACGT 2 cut(s) 1294, 2231
Kpn2I TCCGGA 1 cut(s) 400
KpnI GGTACC 1 cut(s) 2281
Ksp22I TGATCA 2 cut(s) 678, 1132
LmnI GCTCC 4 cut(s) 928, 1174, 2008, 2164
Lsp1109I GCAGC 6 cut(s) 1237, 1240, 1676, 1760, 1782, 2002
LweI GCATC 9 cut(s) 198, 406, 523, 577, 910, 1243, 1614, 1684, 1717
MaeI CTAG 5 cut(s) 390, 933, 2313, 2468, 2804
MaeII ACGT 2 cut(s) 1294, 2231
MfeI CAATTG 2 cut(s) 696, 2019
MflI RGATCY 6 cut(s) 460, 908, 1403, 1820, 2169, 2856
MhlI GDGCHC 5 cut(s) 708, 974, 1652, 2013, 2756
MlyI GAGTC 7 cut(s) 332, 429, 747, 934, 1200, 1876, 2120
MmeI TCCRAC 3 cut(s) 896, 1381, 1395
Mph1103I ATGCAT 6 cut(s) 399, 748, 892, 1637, 1673, 1677
MroI TCCGGA 1 cut(s) 400
MroXI GAANNNNTTC 2 cut(s) 825, 1861
MslI CAYNNNNRTG 3 cut(s) 761, 1146, 2486
MspI CCGG 2 cut(s) 94, 401
MspR9I CCNGG 7 cut(s) 94, 95, 520, 735, 1038, 1600, 2163
MunI CAATTG 2 cut(s) 696, 2019
Mva1269I GAATGC 8 cut(s) 37, 599, 746, 867, 892, 1472, 1776, 1836
MvaI CCWGG 5 cut(s) 520, 735, 1038, 1600, 2163
MvnI CGCG 2 cut(s) 1782, 1936
MwoI GCNNNNNNNGC 7 cut(s) 113, 1005, 1647, 1779, 1942, 2060, 2449
NciI CCSGG 2 cut(s) 94, 95
NlaIV GGNNCC 7 cut(s) 462, 1849, 2053, 2069, 2139, 2160, 2279
NmuCI GTSAC 5 cut(s) 514, 766, 1448, 1578, 2123
NruI TCGCGA 1 cut(s) 1782
NsiI ATGCAT 6 cut(s) 399, 748, 892, 1637, 1673, 1677
NspI RCATGY 5 cut(s) 230, 397, 1639, 1671, 1675
NspV TTCGAA 1 cut(s) 1928
PaeI GCATGC 4 cut(s) 230, 397, 1671, 1675
PaeR7I CTCGAG 1 cut(s) 1866
PctI GAATGC 8 cut(s) 37, 599, 746, 867, 892, 1472, 1776, 1836
PdmI GAANNNNTTC 2 cut(s) 825, 1861
PfeI GAWTC 8 cut(s) 539, 856, 1925, 2360, 2378, 2413, 2455, 2848
PflMI CCANNNNNTGG 1 cut(s) 2668
PfoI TCCNGGA 3 cut(s) 733, 1598, 2161
PkrI GCNGC 8 cut(s) 171, 599, 1252, 1255, 1666, 1775, 1797, 2017
PleI GAGTC 7 cut(s) 331, 429, 746, 934, 1199, 1876, 2120
PpsI GAGTC 7 cut(s) 331, 429, 746, 934, 1199, 1876, 2120
PsiI TTATAA 1 cut(s) 785
Psp124BI GAGCTC 3 cut(s) 974, 1652, 2013
Psp6I CCWGG 5 cut(s) 518, 733, 1036, 1598, 2161
PspEI GGTNACC 1 cut(s) 1892
PspGI CCWGG 5 cut(s) 518, 733, 1036, 1598, 2161
PspN4I GGNNCC 7 cut(s) 462, 1849, 2053, 2069, 2139, 2160, 2279
PspPI GGNCC 3 cut(s) 1847, 1955, 2474
PsrI GAACNNNNNNTAC 2 cut(s) 156, 188
PstI CTGCAG 3 cut(s) 1762, 2261, 2856
PstNI CAGNNNCTG 2 cut(s) 166, 2363
PsuI RGATCY 6 cut(s) 460, 908, 1403, 1820, 2169, 2856
RruI TCGCGA 1 cut(s) 1782
RsaI GTAC 6 cut(s) 15, 50, 312, 1290, 2279, 2695
RsaNI GTAC 6 cut(s) 14, 49, 311, 1289, 2278, 2694
RseI CAYNNNNRTG 3 cut(s) 761, 1146, 2486
SacI GAGCTC 3 cut(s) 974, 1652, 2013
SatI GCNGC 8 cut(s) 170, 598, 1251, 1254, 1665, 1774, 1796, 2016
Sau96I GGNCC 3 cut(s) 1847, 1955, 2474
SchI GAGTC 7 cut(s) 332, 429, 747, 934, 1200, 1876, 2120
ScrFI CCNGG 7 cut(s) 94, 95, 520, 735, 1038, 1600, 2163
SduI GDGCHC 5 cut(s) 708, 974, 1652, 2013, 2756
SfaNI GCATC 9 cut(s) 198, 406, 523, 577, 910, 1243, 1614, 1684, 1717
SfcI CTRYAG 4 cut(s) 1758, 1999, 2257, 2852
Sfr274I CTCGAG 1 cut(s) 1866
SfuI TTCGAA 1 cut(s) 1928
SinI GGWCC 2 cut(s) 1847, 2474
SlaI CTCGAG 1 cut(s) 1866
SmaI CCCGGG 1 cut(s) 95
SmiI ATTTAAAT 1 cut(s) 1750
SmiMI CAYNNNNRTG 3 cut(s) 761, 1146, 2486
SmlI CTYRAG 7 cut(s) 74, 1070, 1514, 1574, 1866, 2119, 2446
SmoI CTYRAG 7 cut(s) 74, 1070, 1514, 1574, 1866, 2119, 2446
SphI GCATGC 4 cut(s) 230, 397, 1671, 1675
SsiI CCGC 6 cut(s) 169, 356, 597, 634, 1532, 2682
SspI AATATT 1 cut(s) 272
SspMI CTAG 5 cut(s) 390, 933, 2313, 2468, 2804
SstI GAGCTC 3 cut(s) 974, 1652, 2013
StyD4I CCNGG 7 cut(s) 92, 93, 518, 733, 1036, 1598, 2161
StyI CCWWGG 4 cut(s) 464, 1381, 1807, 2518
SwaI ATTTAAAT 1 cut(s) 1750
TaaI ACNGT 6 cut(s) 11, 478, 1125, 1432, 1833, 2631
TaiI ACGT 2 cut(s) 1297, 2234
TaqI TCGA 8 cut(s) 861, 1646, 1681, 1867, 1928, 2034, 2093, 2104
TatI WGTACW 3 cut(s) 13, 310, 1288
TauI GCSGC 2 cut(s) 172, 600
TfiI GAWTC 8 cut(s) 539, 856, 1925, 2360, 2378, 2413, 2455, 2848
TscAI CASTG 9 cut(s) 16, 137, 481, 1370, 1737, 1838, 1916, 2148, 2761
TseFI GTSAC 5 cut(s) 514, 766, 1448, 1578, 2123
TseI GCWGC 6 cut(s) 1250, 1253, 1664, 1773, 1795, 2015
Tsp45I GTSAC 5 cut(s) 514, 766, 1448, 1578, 2123
TspMI CCCGGG 1 cut(s) 93
TspRI CASTG 9 cut(s) 16, 137, 481, 1370, 1737, 1838, 1916, 2148, 2761
Van91I CCANNNNNTGG 1 cut(s) 2668
VneI GTGCAC 1 cut(s) 2752
VpaK11BI GGWCC 2 cut(s) 1847, 2474
XagI CCTNNNNNAGG 1 cut(s) 814
XapI RAATTY 8 cut(s) 405, 581, 1751, 1973, 2207, 2644, 2651, 2807
XceI RCATGY 5 cut(s) 230, 397, 1639, 1671, 1675
XhoI CTCGAG 1 cut(s) 1866
XmaI CCCGGG 1 cut(s) 93
XmiI GTMKAC 1 cut(s) 2529
XmnI GAANNNNTTC 2 cut(s) 825, 1861
XspI CTAG 5 cut(s) 390, 933, 2313, 2468, 2804
Zsp2I ATGCAT 6 cut(s) 399, 748, 892, 1637, 1673, 1677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.