Rmu_sc0007286.1_g000032

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007286.1
OG0000526
Physical Location & Seq
Reverse (-)
88877 .. 92650
3774 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0007286.1_g000032.1.cds

Sequence Viewer

Length: 3774 bp
atgcctgcctgttggtctattttgaggaaggcgagggttcaaggtgtaagctggattaagctttcatcacatggaagatttgttctgtcttattcctttgacagcaaggaatatgatcctatactaagtttccttggagttgaactagtgaatgatgcatggtatgttaaatgcatacagggtacttctgatgttgttgatcgagtgtcagaagatgtgtacttagagcttctactgtttatcgctgacaattgggcttctaaatttggtcacaccaacatgaagaacataccattgataaaatatgcagactgtagaaatgaatctttatgcagcataagcgaaatccagaaaggtgaatgtagagttttcttatcgagcaaaagttctcatgtttcatggctgattgattgcaaccgggaatttaattctgtgactcgtcttctctttatgccaaaagccactcaggaagccatctggacatgctctgggaaggatacattgaagaaatggctagctgatcaagctaaggttgtagatgttgatgtcaatcaatatgcagttgatctccttcagaattctcttaatgagcggaagcttgttattgcatatgttcatttcttatatcactcggttgataataattttatctcaagtcgggaagttgaccgtttatgtggaattatgccactagtggatggctatgggaacatcatcagaagtagaaaaggcgttgttgttgttcctgccaatggaagcaaatgggcgggattgactgattctaatttgtggagaaaagaaggctatgttgaattaggagagcattatatgaattctggctattttgctggtaagtatacactggagaagaagcttcttgagttccttatagaacatgctgctgctttggatgtccctcatatttctgctccaagtgatcgtatctcgtctgtatctgcaccactaactaagcagaatgccttcttgcttttggattggatttgtcaactgaattataaaagggtttgcatccctcagaagttcttggcttgcataaaggaaggtagttggctgaaagttactttgaatggctctcctggttataggccaccatcccagtcatttctgcttaactcatcatggggaaatactttgcagaatggatccgtctctgttgacattccattgattgatacgagcttttatggtgagagcattagtggatacaaggaggagctaaagacaattggggtcatgtttgaatatagcgaagcatgtgacttcattgggaagcatttcatgtctctggcagcttcatcctctttaactagaggaaatgtgcattccattctgcgtttcatcaggtttttaagggacaaacttctttctccggccgattttatctctgctattaagaaagggaactggctgaagacttccgttggctacaggtctcctgtaggatctgttttgtcagataaggagtggaatgttgcctccaaaattagcaacattcccttcattgatcaggacttttacggagatgaaatctgtaatttcagaactgaacttgggctgcttggtgttataatctccttcaacagaagttaccaacttataattgacaacctgaagccaccatcttgcataacttcttggacacctgaggttgttaccttaatgctcaactgtatggctctatccaaatcatctgacaaacttgtcaatgcactgaaaggagtcaagtgcctgaagacaattattggttacaagtgtccaggggaatgtctcttatttcatcacgaatggggttgcattcttcaggtcgtcaatggtcttcccgtgattgatcatgaattctatgggagcaacattttccggtacataaatgaactgaaaaaaattggtgtggtggttgattttgaaggggctgcaaaagtacttgctcgatatttcaggcactatgcaaaatcagcttccatcaccaaggaaaatgttgcatcattgcttctgtgttacagagagctgaatgggactccatataaatttcctgctgatattaggcgctgcttcgctaaggtcaagtggttgcgtagtcgttttggtgattacagatgtccaagggagtgcattctctttagtcccgagtgggaatctatatccccaataactctgcttcctttcattgatgattctgacagttactatggaaagggcatacatgagtacagaaaagagttgaagagcttgggggttgtcgtcgaatacacggaaggagttaagtttgtggctcgtggtctttatctccctagggatgccagcagtatttttcctgtaaatgcacttgcgttgctggaatgcatacgtgttttccagaagaaaggttacgcctttcttgataattctgatttcatgaagaaagtttctcaaccatggataaagactcatgctggttattggtctcccaaagagtctttgttgtttgattccaagtttggcttatatttgaagcggactgatgggcctttcattgatgaagaattctatggtactaagattgcatcttacaaaaaagagctgtctgcattgggggttattgttgaagctgaaaagggatgtcccttgattgccggccaccttgatattcatgatgagactgaagcttttgttcggttgtataattacttgagtgagttccagtgggagccagacactgacgctgctcgaaggatctggattccaaaaggggagtgggttcgtgctgatgaatgtgttatttatgacaaggatgggctatttggtttgcagttgactgttttggagaaacattatgagcataaacttctgcttttcttctccagtgcgtttggagttaaatctcatccttcagttgatgattacttgaaggtttggaaggtctgggaaagttctggaagtgcattgtcgcatgctgattgttgcaagttttggagttatatctcgaagcactggaattcaaagacagaaaagactgttgctgatgccttggtgaaagtacctgtcagttctggatctgaaggaatcttgctgtgctacaagtccgatgttttcattgctgatgaccttcatctgaagtatctatttgaacagtcttctcccgagtctatttttgtttggtaccctcaaccaagtttgccttccattccaagatccaagttgcttgacatttatcgaagaattggtgttcgcactatatctgaatctgtacaaaaggaagaggcgtcacttgcagatgatgttgaagcagtctctttgccgagggaaaagctgattaagaaagcgctgttcaggccgattcttggttttcttgctgctcctcccatggaaatggaagctgagaagaggcttgaagctgttcaattccttgtgaatgttactgtggtggttacagctgaaccaatctctgtaagctatagtctacctttatcttcttggaaagttttgaatgtgagaggaagccaaaagatacgttttgatagagacaattcaaagatcttcactcagaaaatggacaagtctggagggcagaagagtatgtttgagtttgctacttctttctctcaagcaatatctgagagtgtggtatgggatagtacggatcatatagattcactctctgagctgatcaaggtggctgctttgctaaacttcaaagaggaagcagttgattccttgatgaagtcaaagaatttgcaaatcttcatggaggatgaggagtttctcaggactgtctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1257

Amino Acids

142.58

Weight (kDa)

7.45

Isoelectric Point (pI)

36.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28020 AT3G48770 AT3G48770
fragaria_vesca FvH4_5g09020 FvH4_5g09020
malus_domestica MD06G1092400.v1.1 MD06G1134600.v1.1 MD06G1134700.v1.1 MD06G1134800.v1.1 MD06G1135200.v1.1 MD06G1135400.v1.1 MD06G1135500.v1.1 MD08G1137600.v1.1 MD15G1395100.v1.1
prunus_persica Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145600_v2.0.a1 Prupe.5G145800_v2.0.a1 Prupe.5G145900_v2.0.a1
pyrus_communis pycom06g12550 pycom06g12570 pycom06g12580 pycom06g12590 pycom06g12610 pycom06g12630 pycom06g12640 pycom06g12650 pycom08g06280
rosa_chinensis RchiOBHm_Chr6g0249321 RchiOBHm_Chr6g0275111 RchiOBHm_Chr7g0189191 RchiOBHm_Chr7g0189261
rosa_laevigata RLG00000004622 RLG00000004623 RLG00000008237 RLG00000008238 RLG00000023303 RLG00000035187
rosa_multiflora Rmu_co8294311.1_g000001 Rmu_co8389833.1_g000001 Rmu_sc0001044.1_g000004 Rmu_sc0001329.1_g000011 Rmu_sc0006012.1_g000010 Rmu_sc0007286.1_g000032 Rmu_sc0009360.1_g000016 Rmu_sc0011817.1_g000001 Rmu_sc0015050.1_g000001
rosa_roxburghii Rroxscaffold_1G00064000 Rroxscaffold_3G00265460 Rroxscaffold_3G00265580 Rroxscaffold_3G00265640 Rroxscaffold_3G00265690 Rroxscaffold_3G00265700 Rroxscaffold_7G00193620 Rroxscaffold_7G00204550 Rroxscaffold_7G00217350
rosa_rugosa Rorug01G0465500 Rorug06G0088500 Rorug06G0496700 Rorug06G0496700
rosa_samantha Rh1AG064800 Rh2BG015100 Rh2BG493500 Rh2DG017300 Rh2DG502500 Rh4BG208500 Rh5BG528500 Rh5CG552900 Rh6CG207800 Rh6DG197900 Rh6DG198000 Rh7AG101300 Rh7AG101400 Rh7AG101600 Rh7BG104100 Rh7BG104200 Rh7CG105600 Rh7CG105700 Rh7DG103200 Rh7DG103400
rosa_wichuraiana Rw1G007300 Rw5G034670 Rw7G008750 Rw7G008780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1017, 1589, 1619
AasI GACNNNNNNGTC 1 cut(s) 1721
Acc65I GGTACC 1 cut(s) 3156
AccB1I GGYRCC 1 cut(s) 3156
AccBSI CCGCTC 1 cut(s) 592
AccI GTMKAC 2 cut(s) 857, 3457
AciI CCGC 3 cut(s) 592, 767, 2512
AclWI GGATC 8 cut(s) 110, 1158, 1171, 1471, 2738, 3058, 3183, 3645
AcoI YGGCCR 2 cut(s) 1392, 2632
AcsI RAATTY 9 cut(s) 263, 422, 577, 832, 1856, 2045, 2540, 2992, 3727
AcuI CTGAAG 9 cut(s) 557, 1451, 1652, 1772, 1805, 2679, 2871, 3075, 3131
AcyI GRCGYC 1 cut(s) 3260
AfeI AGCGCT 1 cut(s) 3321
AfiI CCNNNNNNNGG 2 cut(s) 752, 3338
AflIII ACRYGT 1 cut(s) 2365
AhlI ACTAGT 2 cut(s) 145, 691
AjnI CCWGG 2 cut(s) 1096, 1777
AjuI GAANNNNNNNTTGG 2 cut(s) 3160, 3192
AloI GAACNNNNNNTCC 2 cut(s) 66, 98
Alw26I GTCTC 8 cut(s) 1174, 1308, 1458, 1793, 2466, 2648, 3292, 3513
AlwI GGATC 8 cut(s) 110, 1158, 1171, 1471, 2738, 3058, 3183, 3645
AlwNI CAGNNNCTG 1 cut(s) 2714
Ama87I CYCGRG 2 cut(s) 2144, 3137
Aor51HI AGCGCT 1 cut(s) 3321
AoxI GGCC 5 cut(s) 1106, 1392, 2522, 2632, 3329
ApoI RAATTY 9 cut(s) 263, 422, 577, 832, 1856, 2045, 2540, 2992, 3727
Asp700I GAANNNNTTC 3 cut(s) 980, 1295, 3393
Asp718I GGTACC 1 cut(s) 3156
AspA2I CCTAGG 1 cut(s) 2309
AspLEI GCGC 2 cut(s) 2067, 3322
AspS9I GGNCC 1 cut(s) 2522
AsuC2I CCSGG 1 cut(s) 419
AsuHPI GGTGA 5 cut(s) 368, 1220, 1975, 2117, 3040
AsuNHI GCTAGC 1 cut(s) 514
AvaI CYCGRG 2 cut(s) 2144, 3137
AvrII CCTAGG 1 cut(s) 2309
AxyI CCTNAGG 1 cut(s) 1665
BamHI GGATCC 1 cut(s) 1163
BanI GGYRCC 1 cut(s) 3156
BarI GAAGNNNNNNTAC 2 cut(s) 2369, 2401
BauI CACGAG 1 cut(s) 2292
BbsI GAAGAC 5 cut(s) 434, 1439, 1760, 1829, 3123
BccI CCATC 7 cut(s) 482, 692, 1120, 1648, 1988, 2513, 2783
BciT130I CCWGG 2 cut(s) 1098, 1779
BciVI GTATCC 2 cut(s) 490, 1217
BclI TGATCA 4 cut(s) 520, 1525, 1849, 3663
BcnI CCSGG 1 cut(s) 419
BcoDI GTCTC 8 cut(s) 1174, 1308, 1458, 1793, 2466, 2648, 3292, 3513
BcuI ACTAGT 2 cut(s) 145, 691
BfaI CTAG 6 cut(s) 146, 515, 692, 1329, 2310, 3772
BfmI CTRYAG 4 cut(s) 313, 1447, 1458, 3451
BfoI RGCGCY 2 cut(s) 2068, 3323
BfuI GTATCC 2 cut(s) 490, 1217
BglII AGATCT 1 cut(s) 3531
BlnI CCTAGG 1 cut(s) 2309
BmcAI AGTACT 1 cut(s) 1941
Bme1390I CCNGG 3 cut(s) 419, 1098, 1779
BmeT110I CYCGRG 2 cut(s) 2144, 3137
BmgT120I GGNCC 1 cut(s) 2522
BmiI GGNNCC 3 cut(s) 1165, 2706, 3158
BmrFI CCNGG 3 cut(s) 419, 1098, 1779
BmrI ACTGGG 1 cut(s) 1111
BmsI GCATC 6 cut(s) 145, 1038, 2009, 2305, 2570, 3010
BmtI GCTAGC 1 cut(s) 518
BmuI ACTGGG 1 cut(s) 1111
BpiI GAAGAC 5 cut(s) 434, 1439, 1760, 1829, 3123
BpmI CTGGAG 3 cut(s) 884, 2842, 3579
Bpu10I CCTNAGC 2 cut(s) 528, 2076
BpuEI CTTGAG 4 cut(s) 637, 899, 2707, 3585
BpuMI CCSGG 1 cut(s) 419
BsaAI YACGTR 1 cut(s) 2366
BsaBI GATNNNNATC 1 cut(s) 549
BsaHI GRCGYC 1 cut(s) 3260
BsaI GGTCTC 2 cut(s) 1458, 2466
BsaJI CCNNGG 9 cut(s) 133, 1778, 1986, 2120, 2309, 2432, 3024, 3296, 3360
BsaWI WCCGGW 1 cut(s) 1878
Bsc4I CCNNNNNNNGG 2 cut(s) 752, 3338
Bse118I RCCGGY 1 cut(s) 2630
Bse1I ACTGG 6 cut(s) 867, 1117, 1430, 2698, 2859, 2993
Bse21I CCTNAGG 1 cut(s) 1665
Bse3DI GCAATG 2 cut(s) 2003, 3090
Bse8I GATNNNNATC 1 cut(s) 549
BseBI CCWGG 2 cut(s) 1098, 1779
BseDI CCNNGG 9 cut(s) 133, 1778, 1986, 2120, 2309, 2432, 3024, 3296, 3360
BseJI GATNNNNATC 1 cut(s) 549
BseLI CCNNNNNNNGG 2 cut(s) 752, 3338
BseMI GCAATG 2 cut(s) 2003, 3090
BseMII CTCAG 7 cut(s) 479, 1049, 1656, 3366, 3554, 3603, 3648
BseNI ACTGG 6 cut(s) 867, 1117, 1430, 2698, 2859, 2993
BseRI GAGGAG 3 cut(s) 1247, 3345, 3767
BseX3I CGGCCG 1 cut(s) 1392
BsgI GTGCAG 1 cut(s) 942
Bsh1285I CGRYCG 1 cut(s) 1395
BshFI GGCC 5 cut(s) 1108, 1394, 2524, 2634, 3331
BshNI GGYRCC 1 cut(s) 3156
BsiEI CGRYCG 1 cut(s) 1395
BsiHKCI CYCGRG 2 cut(s) 2144, 3137
BsiSI CCGG 4 cut(s) 418, 1391, 1879, 2631
BslFI GGGAC 5 cut(s) 899, 1388, 2047, 2127, 2604
BslI CCNNNNNNNGG 2 cut(s) 752, 3338
BsmAI GTCTC 8 cut(s) 1174, 1308, 1458, 1793, 2466, 2648, 3292, 3513
BsmBI CGTCTC 1 cut(s) 1174
BsmFI GGGAC 5 cut(s) 899, 1388, 2047, 2127, 2604
BsmI GAATGC 5 cut(s) 982, 1342, 1815, 2130, 2363
BsnI GGCC 5 cut(s) 1108, 1394, 2524, 2634, 3331
Bso31I GGTCTC 2 cut(s) 1458, 2466
BsoBI CYCGRG 2 cut(s) 2144, 3137
Bsp1407I TGTACA 1 cut(s) 3244
Bsp19I CCATGG 2 cut(s) 2432, 3360
BspACI CCGC 3 cut(s) 592, 767, 2512
BspANI GGCC 5 cut(s) 1108, 1394, 2524, 2634, 3331
BspCNI CTCAG 8 cut(s) 478, 1048, 1657, 3367, 3553, 3604, 3649, 3774
BspHI TCATGA 3 cut(s) 1852, 2412, 2647
BspLI GGNNCC 3 cut(s) 1165, 2706, 3158
BspOI GCTAGC 1 cut(s) 518
BspPI GGATC 8 cut(s) 110, 1158, 1171, 1471, 2738, 3058, 3183, 3645
BspQI GCTCTTC 1 cut(s) 2237
BspT107I GGYRCC 1 cut(s) 3156
BspTNI GGTCTC 2 cut(s) 1458, 2466
BsrBI CCGCTC 1 cut(s) 592
BsrDI GCAATG 2 cut(s) 2003, 3090
BsrFI RCCGGY 1 cut(s) 2630
BsrGI TGTACA 1 cut(s) 3244
BsrI ACTGG 6 cut(s) 867, 1117, 1430, 2698, 2859, 2993
BssAI RCCGGY 1 cut(s) 2630
BssECI CCNNGG 9 cut(s) 133, 1778, 1986, 2120, 2309, 2432, 3024, 3296, 3360
BssNAI GTATAC 1 cut(s) 858
BssNI GRCGYC 1 cut(s) 3260
BssSI CACGAG 1 cut(s) 2292
BssT1I CCWWGG 7 cut(s) 133, 1986, 2120, 2309, 2432, 3024, 3360
Bst1107I GTATAC 1 cut(s) 858
Bst2BI CACGAG 1 cut(s) 2292
Bst2UI CCWGG 2 cut(s) 1098, 1779
Bst6I CTCTTC 4 cut(s) 2237, 3249, 3374, 3563
BstACI GRCGYC 1 cut(s) 3260
BstAUI TGTACA 1 cut(s) 3244
BstBAI YACGTR 1 cut(s) 2366
BstC8I GCNNGC 6 cut(s) 6, 516, 1051, 2320, 2632, 2949
BstDSI CCRYGG 2 cut(s) 2432, 3360
BstH2I RGCGCY 2 cut(s) 2068, 3323
BstHHI GCGC 2 cut(s) 2067, 3322
BstMAI GTCTC 8 cut(s) 1174, 1308, 1458, 1793, 2466, 2648, 3292, 3513
BstMCI CGRYCG 1 cut(s) 1395
BstMWI GCNNNNNNNGC 5 cut(s) 339, 524, 1973, 3266, 3328
BstNI CCWGG 2 cut(s) 1098, 1779
BstNSI RCATGY 4 cut(s) 486, 899, 1278, 2951
BstSCI CCNGG 3 cut(s) 417, 1096, 1777
BstSFI CTRYAG 4 cut(s) 313, 1447, 1458, 3451
BstV2I GAAGAC 5 cut(s) 434, 1439, 1760, 1829, 3123
BstX2I RGATCY 6 cut(s) 1163, 1463, 2730, 3050, 3188, 3531
BstXI CCANNNNNNTGG 1 cut(s) 3367
BstYI RGATCY 6 cut(s) 1163, 1463, 2730, 3050, 3188, 3531
BstZ17I GTATAC 1 cut(s) 858
BstZI CGGCCG 1 cut(s) 1392
Bsu36I CCTNAGG 1 cut(s) 1665
BsuI GTATCC 2 cut(s) 490, 1217
BsuRI GGCC 5 cut(s) 1108, 1394, 2524, 2634, 3331
BtgI CCRYGG 2 cut(s) 2432, 3360
BtsIMutI CAGTG 6 cut(s) 860, 1730, 2705, 2712, 2866, 2986
Cac8I GCNNGC 6 cut(s) 6, 516, 1051, 2320, 2632, 2949
CaiI CAGNNNCTG 1 cut(s) 2714
CciI TCATGA 3 cut(s) 1852, 2412, 2647
CfoI GCGC 2 cut(s) 2067, 3322
Cfr10I RCCGGY 1 cut(s) 2630
Cfr13I GGNCC 1 cut(s) 2522
CseI GACGC 2 cut(s) 2726, 3249
DrdI GACNNNNNNGTC 1 cut(s) 1721
DseDI GACNNNNNNGTC 1 cut(s) 1721
EaeI YGGCCR 2 cut(s) 1392, 2632
EagI CGGCCG 1 cut(s) 1392
Eam1104I CTCTTC 4 cut(s) 2237, 3249, 3374, 3563
EarI CTCTTC 4 cut(s) 2237, 3249, 3374, 3563
EclXI CGGCCG 1 cut(s) 1392
Eco130I CCWWGG 7 cut(s) 133, 1986, 2120, 2309, 2432, 3024, 3360
Eco31I GGTCTC 2 cut(s) 1458, 2466
Eco47III AGCGCT 1 cut(s) 3321
Eco52I CGGCCG 1 cut(s) 1392
Eco57I CTGAAG 9 cut(s) 557, 1451, 1652, 1772, 1805, 2679, 2871, 3075, 3131
Eco81I CCTNAGG 1 cut(s) 1665
Eco88I CYCGRG 2 cut(s) 2144, 3137
EcoRI GAATTC 5 cut(s) 577, 832, 1856, 2540, 2992
EcoRII CCWGG 2 cut(s) 1096, 1777
EcoT14I CCWWGG 7 cut(s) 133, 1986, 2120, 2309, 2432, 3024, 3360
EcoT22I ATGCAT 3 cut(s) 160, 176, 2363
ErhI CCWWGG 7 cut(s) 133, 1986, 2120, 2309, 2432, 3024, 3360
Esp3I CGTCTC 1 cut(s) 1174
FalI AAGNNNNNCTT 4 cut(s) 2483, 2515, 3160, 3192
FaqI GGGAC 5 cut(s) 899, 1388, 2047, 2127, 2604
FauI CCCGC 1 cut(s) 760
FauNDI CATATG 1 cut(s) 610
FbaI TGATCA 4 cut(s) 520, 1525, 1849, 3663
FblI GTMKAC 2 cut(s) 857, 3457
FspBI CTAG 6 cut(s) 146, 515, 692, 1329, 2310, 3772
GlaI GCGC 2 cut(s) 2066, 3321
GsuI CTGGAG 3 cut(s) 884, 2842, 3579
HaeII RGCGCY 2 cut(s) 2068, 3323
HaeIII GGCC 5 cut(s) 1108, 1394, 2524, 2634, 3331
HapII CCGG 4 cut(s) 418, 1391, 1879, 2631
HgaI GACGC 2 cut(s) 2726, 3249
HhaI GCGC 2 cut(s) 2067, 3322
Hin1I GRCGYC 1 cut(s) 3260
Hin6I GCGC 2 cut(s) 2065, 3320
HinP1I GCGC 2 cut(s) 2065, 3320
HincII GTYRAC 4 cut(s) 667, 1007, 1177, 2811
HindII GTYRAC 4 cut(s) 667, 1007, 1177, 2811
HindIII AAGCTT 4 cut(s) 59, 596, 872, 2661
HpaII CCGG 4 cut(s) 418, 1391, 1879, 2631
HphI GGTGA 5 cut(s) 368, 1220, 1975, 2117, 3040
Hpy166II GTNNAC 7 cut(s) 220, 667, 858, 1007, 1177, 2811, 3458
Hpy8I GTNNAC 7 cut(s) 220, 667, 858, 1007, 1177, 2811, 3458
Hpy99I CGWCG 1 cut(s) 2264
HpyCH4IV ACGT 2 cut(s) 2365, 3508
HpyF10VI GCNNNNNNNGC 5 cut(s) 339, 524, 1973, 3266, 3328
HpySE526I ACGT 2 cut(s) 2365, 3508
Hsp92I GRCGYC 1 cut(s) 3260
HspAI GCGC 2 cut(s) 2065, 3320
KpnI GGTACC 1 cut(s) 3160
KroI GCCGGC 1 cut(s) 2630
KroNI GCCGGC 1 cut(s) 2632
Ksp22I TGATCA 4 cut(s) 520, 1525, 1849, 3663
LguI GCTCTTC 1 cut(s) 2237
LmnI GCTCC 5 cut(s) 934, 1234, 1866, 2704, 3358
LweI GCATC 6 cut(s) 145, 1038, 2009, 2305, 2570, 3010
MaeI CTAG 6 cut(s) 146, 515, 692, 1329, 2310, 3772
MaeII ACGT 2 cut(s) 2365, 3508
MbiI CCGCTC 1 cut(s) 592
MfeI CAATTG 2 cut(s) 250, 1245
MflI RGATCY 6 cut(s) 1163, 1463, 2730, 3050, 3188, 3531
MlyI GAGTC 6 cut(s) 430, 1749, 2029, 2437, 2480, 3149
Mph1103I ATGCAT 3 cut(s) 160, 176, 2363
MroNI GCCGGC 1 cut(s) 2630
MroXI GAANNNNTTC 3 cut(s) 980, 1295, 3393
MslI CAYNNNNRTG 2 cut(s) 278, 3365
MspA1I CMGCKG 1 cut(s) 3431
MspI CCGG 4 cut(s) 418, 1391, 1879, 2631
MspR9I CCNGG 3 cut(s) 419, 1098, 1779
MunI CAATTG 2 cut(s) 250, 1245
Mva1269I GAATGC 5 cut(s) 982, 1342, 1815, 2130, 2363
MvaI CCWGG 2 cut(s) 1098, 1779
MwoI GCNNNNNNNGC 5 cut(s) 339, 524, 1973, 3266, 3328
NaeI GCCGGC 1 cut(s) 2632
NciI CCSGG 1 cut(s) 419
NcoI CCATGG 2 cut(s) 2432, 3360
NdeI CATATG 1 cut(s) 610
NgoMIV GCCGGC 1 cut(s) 2630
NheI GCTAGC 1 cut(s) 514
NlaIV GGNNCC 3 cut(s) 1165, 2706, 3158
NmeAIII GCCGAG 1 cut(s) 3321
NmuCI GTSAC 4 cut(s) 269, 433, 1277, 3261
NsiI ATGCAT 3 cut(s) 160, 176, 2363
NspI RCATGY 4 cut(s) 486, 899, 1278, 2951
PaeI GCATGC 1 cut(s) 2951
PagI TCATGA 3 cut(s) 1852, 2412, 2647
PciSI GCTCTTC 1 cut(s) 2237
PctI GAATGC 5 cut(s) 982, 1342, 1815, 2130, 2363
PdiI GCCGGC 1 cut(s) 2632
PdmI GAANNNNTTC 3 cut(s) 980, 1295, 3393
PleI GAGTC 6 cut(s) 430, 1748, 2029, 2437, 2479, 3148
PpsI GAGTC 6 cut(s) 430, 1748, 2029, 2437, 2479, 3148
Ppu21I YACGTR 1 cut(s) 2366
PsiI TTATAA 3 cut(s) 1017, 1589, 1619
Psp6I CCWGG 2 cut(s) 1096, 1777
PspGI CCWGG 2 cut(s) 1096, 1777
PspN4I GGNNCC 3 cut(s) 1165, 2706, 3158
PspPI GGNCC 1 cut(s) 2522
PstNI CAGNNNCTG 1 cut(s) 2714
PsuI RGATCY 6 cut(s) 1163, 1463, 2730, 3050, 3188, 3531
PvuII CAGCTG 1 cut(s) 3431
RseI CAYNNNNRTG 2 cut(s) 278, 3365
SapI GCTCTTC 1 cut(s) 2237
Sau96I GGNCC 1 cut(s) 2522
ScaI AGTACT 1 cut(s) 1941
SchI GAGTC 6 cut(s) 430, 1749, 2029, 2437, 2480, 3149
ScrFI CCNGG 3 cut(s) 419, 1098, 1779
SfaNI GCATC 6 cut(s) 145, 1038, 2009, 2305, 2570, 3010
SfcI CTRYAG 4 cut(s) 313, 1447, 1458, 3451
SmiMI CAYNNNNRTG 2 cut(s) 278, 3365
SmlI CTYRAG 4 cut(s) 652, 878, 2686, 3600
SmoI CTYRAG 4 cut(s) 652, 878, 2686, 3600
SpeI ACTAGT 2 cut(s) 145, 691
SphI GCATGC 1 cut(s) 2951
SsiI CCGC 3 cut(s) 592, 767, 2512
SspMI CTAG 6 cut(s) 146, 515, 692, 1329, 2310, 3772
StyD4I CCNGG 3 cut(s) 417, 1096, 1777
StyI CCWWGG 7 cut(s) 133, 1986, 2120, 2309, 2432, 3024, 3360
TaiI ACGT 2 cut(s) 2368, 3511
TaqI TCGA 7 cut(s) 202, 377, 1948, 2262, 2725, 2981, 3211
TatI WGTACW 4 cut(s) 219, 1939, 2226, 3244
TscAI CASTG 6 cut(s) 867, 1737, 2705, 2719, 2866, 2993
TseFI GTSAC 4 cut(s) 269, 433, 1277, 3261
Tsp45I GTSAC 4 cut(s) 269, 433, 1277, 3261
TspGWI ACGGA 5 cut(s) 1156, 1429, 1554, 2285, 3650
TspRI CASTG 6 cut(s) 867, 1737, 2705, 2719, 2866, 2993
XapI RAATTY 9 cut(s) 263, 422, 577, 832, 1856, 2045, 2540, 2992, 3727
XceI RCATGY 4 cut(s) 486, 899, 1278, 2951
XcmI CCANNNNNNNNNTGG 1 cut(s) 2748
XmaJI CCTAGG 1 cut(s) 2309
XmiI GTMKAC 2 cut(s) 857, 3457
XmnI GAANNNNTTC 3 cut(s) 980, 1295, 3393
XspI CTAG 6 cut(s) 146, 515, 692, 1329, 2310, 3772
ZrmI AGTACT 1 cut(s) 1941
Zsp2I ATGCAT 3 cut(s) 160, 176, 2363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.