MD06G1135200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000526
Physical Location & Seq
Reverse (-)
28018089 .. 28025039
6951 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1135200.v1.1.491

Sequence Viewer

Length: 5175 bp
ATGGCGACGCCGAGAGAGCACATAGAAGAAATACGCAAAGGAAAGTTCTCCATCGGAGAAGAAAAACTCAACCCTTTGAATGAGGATTTTCACCAGGCTGTTAAGAATCTCTCTGCTGAACTCTACGCTAAGGATGTTCACTTCCTCATGGAACTCATCCAGAATGCAGAAGATAATGAGTACCCAGAAGGGGTTGATCCATCACTTGAGTTTGTCATAACTTCCTGGGATATAACAGCCGCGGGGGCTCCTGCTACTTTAATTGTTTTCAACAATGAGAAGGGGTTTTCACCAAAGAACATTGAGTCCATTTGCAGTGTTGGACGTTCCACCAAGAAAGGCAACAGGAAGCGCGGTTACATTGGGGAGAAAGGTATAGGGTTTAAAAGTGTGTTTCTCATCACTGCACACCCCTACATTTTCAGTAATGGTTATCAAATAAGGTTCAGTGAAGAGCCCTGTGTACACTGCAATGTTGGCTACATAGTTCCGGAATGGGTTGATGCAAATCCTACTCTTTTAGACATAAAAAAGATTTATGGTTCCGGTTCTGCCCTTCCAACCACAACACTGATCTTACCTTTGAAGGCAGACAAGTTCCAAGCTGTGAAGCAGCAGCTCTCTAGCATTCACCCTGAGGTTCTATTGTTTCTTTCAAAGATAAAGCGGCTCTCAATCCGCGAAGACAATGAGGATCCAAGGAAAAACAATGTAAGTGCAATTAAGATTGCAAGTGAGACTGAATTTGTGACGAGGAGGAACATTGATGCCCAATCCTACACCCTCCATTTGTCTGCAGAAGAAAATTGTGATGAGGTTGAAAAAGAGTGCAGCTATTATATGTGGAAGCAAAAGTTTCCTGTCAAGCAGGAATACCGAGTTGAACAGAGAAATGAGGTAGAAGAGTGGGTGATCACGCTTGCATTTCCAATTGGGAAACGCCTTCGTAGAGGAATGAACACATGTCCAGGTGTCTACGCCTTCCTACCAACGGAGATGGTGACAAACTTTCCCTTCATAATTCAAGCTGATTTTCTTCTTTCATCATCAAGGGAAACAATACTCCTCGACAACAAATGGAATATGGGGATTCTTGACTGTGTATCAACTACATTTGTCAATGCATTTATCGCGCTTGTTAGATCGATAGACGATGCTCCAGTGTCTAGTTTGCCTTATATGTTCCAGTTCCTGCCTGTCCATAGCTCTGCCTTTGAAGAACTGAATGTTGTTAGGGTGTCAATTAAAGCAAAACTTGTTGAAGAAAACATTGTACCTAGCGAGCCCCACAAGGAGCAGAAATTCTTTCACAAGCCTCGTGAAGTGGGTAGGTTAAGGCCGGATTTTTGGAATATTTTGAGGATGGCGAGGGATCAAGGGATAAGCTTGATTAATCTCTCTTCTCATGGAAAATATGTTCTGTCTTATGCATTTGACCAGGTGGAGTATGATCACATACTGAGTTTCCTTGGTGTTGAACCTGTAAACAGCGAATGGTATGCTAAATGCATCAAGGGGACTTTCGGTCTTGTAACTAGGGTGTCCGAAGACGTCTATTTGGAGCTTCTACTATTTATTTCTGATAACTGGCTGAATAAAATTTTCCAGACCAGCATCAAGAAAGTTCCACTAATAAAATGTTCAGGTTATGGAACCGAATCTTTGTGCCGCATAAGTGCAATCCAGAATGGTGAAAGTAAGGTTTGCGTATCCAGTGATTCTGATCATGTCTCATGGCTGATTGATTGGAATAAGGAGTTTCTACCTGCCGGAAGGCTTCTCTTTATGCCAAAAACCACACAGGAAGCTATCCAGTCTTGCTCTAGGAAAGGGACATTGGAGAAATGGTTTTTAGATCAAGTTAAGGTTCGTTTCGTTAGTGTAAATGAATATGCAAAGGATCTCCTTGACAATTCACTTAATGAGCGGAAGCTTGTGATTGCATTTGCCCACTTCATGTATTTTTCCTGGTGCAGACATTATATCTCGACGAAGAAAGTTAATGAATTATGTGGAGATATGCCGCTAGTGGATAAGTATGGGAAAATCATCAGAACTAGGAAGAAGGTGATTGTGGATAAGTATGGGAACATCATTGAAAACAGGAAGGGGGTTATTGTGCCTGCCAATGGAAGCAAATGGGCAACACTGACTGATTCTAATTTGTGGAGGAAAGAAGGCTACGTTGAGTTAGGAGAAGATTATATGGCTGCAGACTGTTTTGCTGGTAACTGTACAATACAGGAGAACCTCCTAGGGTTCCTTAAAGTCCGTACTGCAGCTACGGACATTCCTTATATACCTGCTCCCAGTGATAGTATATCATCTTTATCTGCACCACTTACTAGGGAAAATGCATTCTTGCTTTTGGACTGGATTCGTCGTCTGGCGCATAAATATGTTTTCGTCTCACAGAATTTTGCGAAGTGCATAGAAGAAGGTGGTTGGCTGAAGGTTACTTTGAATGGCTCTCCTGGTTTTAGGCCACCTTCGCAGTCATTCCTACTTGAGTCATCATCAAGAAACATTTTGCAGAATGTCTCCGTGTTTGTTGATATCCCATTGGTTGATCAGAATTATTATGGTGAGAGGATTAATGAGTACAAGAAGGAGCTAAAAAGAATTGGAGTCAGATTTGAGTTTGGAGAAGCATGCGAGTATCTGGGGAAGCATTTGATGTCTCTTGCTGCTTCATCCACTTTAACCTGTGGCAATGTACTTTCTATACTCCGTTTCATCAAATATTTGAGGGACAACTGTTTTTCTTCTACTGATTTTATCTGTAGTATTAAAGAAGGTCAATGGCTTAAAACGTCCCTTGGCTTCAGGTCTCCGGTGGGTTCTGTTTTGTCGGACAAAGAGTGGATAGTTGCATCACAGATTAGTAACATTCCTTTCATTGATGGAAGATTTTATGGTGAAGAAATTTTTCAGTTCGAAAAAGAACTTGAGTTGCTTGGTGCGGTAGTCAAGTTCGGTGAAAGTTACCAGCTAATCATTGACAACCTAAAGTCACCTTCTTGCTTGACTTCTTGGACACCCGAGATTGTTCTATTCATGCTTGAATGCATGCATATTTCCAGTTCATCTGAGAAACTTGTCAGAGCGTTGAAAGAATCGAATTGCCTGAAGACAAACATTGGTTACAAGCGTCCAGAAGAATGTCTCCTGTTTGACCAGAATTCCGACTGGGGCTGCATTCTTCAGGTCTTTAGTGGTCTTCCATTGATTGATCACGTTTTCTATGGAGACAGGATATTTTCCTACAGAAATGAGTTGAAGAAAACTGGGGTGGTGGTTGATTTTGAGGACGCCACTAAAGTATTTGCTCAATATTTCAAGCGGTATGCATCATCGACTTCCATTACCAATGAAAATGTTGCTTCACTTCTGTTGTGTTACAGAAAGCTGAAGGGAACTCCCTTTAAATTTCCTAAAGATCTTGAGAGCTGCATTCGCCAGGAAAAATGGTTGCGCACTCGCCTTGGTGATTATAGATCTCCCAAAGATTGCATTCTCTTTGGTTCCAGTTGGGAATCTATCTCTCCGATGTGTCTTCTCCCATTCATTGATGATTCTGACAGTTGCTATGGCAAGAACATTCATGATTTTAAGGAAGAACTGAAGAGATTTGGGGTGGTTGTGGAATTCAAGGATGGAGTCAAGTTTGTGGAATCTTGTTTTTATATTCCCTTGAATCTGAGCCACATTTCTCCAGAAAATGCGCTAGCATTGCTGGAATGCTTGCAAATTTTATTGCAGGAGAAAGATTATTCCTTTCCTGAAGTGTTCTTAAAGAAAGTTTCTCAACCATGGTTGAAGACTTATGCTGGCTATAGGCCTCCCAACATGTGCTTGTTGTTTGATTCCAAGTTTGGCTCGTACGTAAAGCAGACTGATGGACCCTTCATTGATGAAGAATTTTATGGATCTAAAATTTTAATGTACAGGAACGAGCTTGCTGAAATAGGGGTGACTATTGAAGTGGAACAAGGGTGTTCTGTTATTGCTAACCATCTTGATCTTCATGATGAATTTTCGACCTTTGTTCGAGTATATAATTACTTGAGCCAGTTTAAGTGGGAGCCAGATAGTGAGGCTGGCCGAAAGATCTGGATTCCGAAAGGAAATAAAAATGGAGAGTGGGTTGGTGCAGATGAATGTGTTATAGACGACAAGGATGAGCTGTTTGGTTTGCAGTTAACTGTTTTGAAGAAACACTATGAGCGAAAATTGCTTAATTTCTTCTGTTGTGCATTTAGAGTACGATCCCAACCTTTGGTTGATGATTACTTGAAGCTTTGGAAAGTTTGGGAAAGTTCTAAAAGAGGATTGTCACATGATCAGTGCTGTAAGTTCTGGCTTTATGTTTCGAAACACTGGAATTTAAAGACGGAGAAAAGTCTTGCTGATGCCCTTTTGAAAGTACCAGTTTATTCAGGCTCCGGTGAAATCTTGTTGTCCAACAAGGAAGATGTTTTCATTGCCGATGACCTTCAGTTACAGTGTCTTTTCGAACAGTCTTCTCACTTGATATTTGCTTGGTACCCTCAGCCAAGCTTGGCTTCCTTGCCTCGAACTAAGCTGCTTGAAATGTACAAGAAAATTGGCGTTCGTGTTATTTCTGAATCTGTGCACAAGGAAGAACTTTCTCTTGCAAACGATGCTGAACTACAAATAATCCCAAGGGAAAGAGTGTTCGCAAAAGCACTTCTGAGGCTGATTCTTGGATTTCTTGCTGGTCCTCCCATTGAAATGGAAGTGGAGAAGAGACAAAAAGCTGTCCAAGGTCTTTTCAATGTTGCTGTATTGGAGACGCATGAACCAATCATTGTAAGTTATGATCTGCCTTTATCTTCTGGGAGAATTTTGAATGTTAGAGGCACACGTAAGATACGGTGGGACAGGAAGAATTCCAAATTCTTCATCCAGAAGATGGACAAATCAGGCGGACAAAAGAGTACCATTGAGTTTGCTACATATTTCGCCGAAGCAGTATCCGATTGTGTACTGTGGGAGTGCACTGAACATATACCTGCACTTTTGGAGCTGATCAAGTGGGCGTTTGTGCTAGAATTCAATGAGGAAGCTGTTGATTTCTTGATGAAGTCGAAGAATCTGCAGATCTTCGTCGAGGATGCAGAGTTTCTGAATTCTGCCTACCCTTCTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1725

Amino Acids

197.52

Weight (kDa)

6.29

Isoelectric Point (pI)

46.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 28 - 144 2.7e-11 Sacsin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28020 AT3G48770 AT3G48770
fragaria_vesca FvH4_5g09020 FvH4_5g09020
malus_domestica MD06G1092400.v1.1 MD06G1134600.v1.1 MD06G1134700.v1.1 MD06G1134800.v1.1 MD06G1135200.v1.1 MD06G1135400.v1.1 MD06G1135500.v1.1 MD08G1137600.v1.1 MD15G1395100.v1.1
prunus_persica Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145600_v2.0.a1 Prupe.5G145800_v2.0.a1 Prupe.5G145900_v2.0.a1
pyrus_communis pycom06g12550 pycom06g12570 pycom06g12580 pycom06g12590 pycom06g12610 pycom06g12630 pycom06g12640 pycom06g12650 pycom08g06280
rosa_chinensis RchiOBHm_Chr6g0249321 RchiOBHm_Chr6g0275111 RchiOBHm_Chr7g0189191 RchiOBHm_Chr7g0189261
rosa_laevigata RLG00000004622 RLG00000004623 RLG00000008237 RLG00000008238 RLG00000023303 RLG00000035187
rosa_multiflora Rmu_co8294311.1_g000001 Rmu_co8389833.1_g000001 Rmu_sc0001044.1_g000004 Rmu_sc0001329.1_g000011 Rmu_sc0006012.1_g000010 Rmu_sc0007286.1_g000032 Rmu_sc0009360.1_g000016 Rmu_sc0011817.1_g000001 Rmu_sc0015050.1_g000001
rosa_roxburghii Rroxscaffold_1G00064000 Rroxscaffold_3G00265460 Rroxscaffold_3G00265580 Rroxscaffold_3G00265640 Rroxscaffold_3G00265690 Rroxscaffold_3G00265700 Rroxscaffold_7G00193620 Rroxscaffold_7G00204550 Rroxscaffold_7G00217350
rosa_rugosa Rorug01G0465500 Rorug06G0088500 Rorug06G0496700 Rorug06G0496700
rosa_samantha Rh1AG064800 Rh2BG015100 Rh2BG493500 Rh2DG017300 Rh2DG502500 Rh4BG208500 Rh5BG528500 Rh5CG552900 Rh6CG207800 Rh6DG197900 Rh6DG198000 Rh7AG101300 Rh7AG101400 Rh7AG101600 Rh7BG104100 Rh7BG104200 Rh7CG105600 Rh7CG105700 Rh7DG103200 Rh7DG103400
rosa_wichuraiana Rw1G007300 Rw5G034670 Rw7G008750 Rw7G008780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1554
Acc16I TGCGCA 1 cut(s) 3476
Acc36I ACCTGC 3 cut(s) 1774, 2311, 5044
Acc65I GGTACC 1 cut(s) 4545
AccB1I GGYRCC 1 cut(s) 4545
AccB7I CCANNNNNTGG 2 cut(s) 4279, 4936
AccBSI CCGCTC 1 cut(s) 1927
AccI GTMKAC 1 cut(s) 975
AccII CGCG 4 cut(s) 242, 354, 681, 1133
AccIII TCCGGA 1 cut(s) 490
AclWI GGATC 7 cut(s) 191, 689, 702, 1380, 1908, 3937, 4263
AcoI YGGCCR 1 cut(s) 4102
AcuI CTGAAG 8 cut(s) 2471, 2809, 3149, 3188, 3431, 3644, 3804, 4481
AcyI GRCGYC 3 cut(s) 8, 1551, 3312
AfiI CCNNNNNNNGG 5 cut(s) 190, 991, 2129, 4279, 4936
AflIII ACRYGT 3 cut(s) 962, 3849, 4886
AhdI GACNNNNNGTC 1 cut(s) 1524
AjnI CCWGG 7 cut(s) 93, 224, 967, 1437, 1967, 2473, 3459
AjuI GAANNNNNNNTTGG 4 cut(s) 1820, 1852, 4549, 4581
AloI GAACNNNNNNTCC 6 cut(s) 290, 322, 1401, 1433, 4682, 4714
Alw21I GWGCWC 3 cut(s) 21, 4638, 5024
Alw44I GTGCAC 2 cut(s) 4634, 5020
AlwI GGATC 7 cut(s) 191, 689, 702, 1380, 1908, 3937, 4263
AlwNI CAGNNNCTG 1 cut(s) 1192
Ama87I CYCGRG 1 cut(s) 3041
Aor13HI TCCGGA 1 cut(s) 490
AoxI GGCC 4 cut(s) 1337, 2483, 3839, 4102
ApaLI GTGCAC 2 cut(s) 4634, 5020
ApeKI GCWGC 9 cut(s) 613, 616, 831, 2210, 2279, 2687, 3195, 3450, 4585
AseI ATTAAT 2 cut(s) 1392, 2595
Asp700I GAANNNNTTC 1 cut(s) 2928
Asp718I GGTACC 1 cut(s) 4545
AspA2I CCTAGG 1 cut(s) 2254
AspLEI GCGC 5 cut(s) 354, 1135, 2392, 3477, 3727
AspS9I GGNCC 2 cut(s) 3902, 4742
AsuII TTCGAA 3 cut(s) 2937, 4373, 4515
AsuNHI GCTAGC 1 cut(s) 3727
AvaI CYCGRG 1 cut(s) 3041
AvaII GGWCC 2 cut(s) 3902, 4742
AvrII CCTAGG 1 cut(s) 2254
AxyI CCTNAGG 1 cut(s) 636
BaeGI GKGCMC 2 cut(s) 4638, 5024
BamHI GGATCC 1 cut(s) 694
BanI GGYRCC 1 cut(s) 4545
BanII GRGCYC 3 cut(s) 250, 459, 1287
BarI GAAGNNNNNNTAC 2 cut(s) 341, 373
BauI CACGAG 1 cut(s) 1317
BbsI GAAGAC 7 cut(s) 690, 1554, 3137, 3212, 3548, 3827, 4515
Bbv12I GWGCWC 3 cut(s) 21, 4638, 5024
BbvCI CCTCAGC 1 cut(s) 4551
BbvI GCAGC 9 cut(s) 625, 628, 843, 2197, 2291, 2674, 3182, 3437, 4572
BccI CCATC 9 cut(s) 59, 208, 991, 1357, 2897, 3650, 3893, 4023, 4930
BcgI CGANNNNNNTGC 4 cut(s) 1481, 1515, 5101, 5135
BciT130I CCWGG 7 cut(s) 95, 226, 969, 1439, 1969, 2475, 3461
BciVI GTATCC 2 cut(s) 1720, 5008
BclI TGATCA 7 cut(s) 912, 1450, 1723, 2569, 3232, 4342, 5052
BfmI CTRYAG 7 cut(s) 795, 2211, 2277, 2782, 3265, 3835, 5120
BfuAI ACCTGC 3 cut(s) 1774, 2311, 5044
BfuI GTATCC 2 cut(s) 1720, 5008
BglI GCCNNNNNGGC 1 cut(s) 245
BglII AGATCT 4 cut(s) 3439, 3497, 4110, 5124
BlnI CCTAGG 1 cut(s) 2254
Bme1390I CCNGG 7 cut(s) 95, 226, 969, 1439, 1969, 2475, 3461
Bme18I GGWCC 2 cut(s) 3902, 4742
BmeRI GACNNNNNGTC 1 cut(s) 1524
BmeT110I CYCGRG 1 cut(s) 3041
BmgT120I GGNCC 2 cut(s) 3902, 4742
BmrFI CCNGG 7 cut(s) 95, 226, 969, 1439, 1969, 2475, 3461
BmrI ACTGGG 3 cut(s) 2304, 3199, 3297
BmtI GCTAGC 1 cut(s) 3731
BmuI ACTGGG 3 cut(s) 2304, 3199, 3297
BpiI GAAGAC 7 cut(s) 690, 1554, 3137, 3212, 3548, 3827, 4515
BpmI CTGGAG 2 cut(s) 1143, 3699
Bpu10I CCTNAGC 2 cut(s) 129, 4551
Bpu14I TTCGAA 3 cut(s) 2937, 4373, 4515
BpuEI CTTGAG 5 cut(s) 227, 2528, 2969, 3464, 4087
Bsa29I ATCGAT 1 cut(s) 1145
BsaAI YACGTR 2 cut(s) 3886, 4889
BsaBI GATNNNNATC 2 cut(s) 507, 1722
BsaHI GRCGYC 3 cut(s) 8, 1551, 3312
BsaI GGTCTC 1 cut(s) 2835
BsaWI WCCGGW 4 cut(s) 490, 545, 2833, 4445
BsaXI ACNNNNNCTCC 4 cut(s) 1832, 1862, 4131, 4161
Bsc4I CCNNNNNNNGG 5 cut(s) 190, 991, 2129, 4279, 4936
Bse21I CCTNAGG 1 cut(s) 636
Bse3DI GCAATG 4 cut(s) 478, 2719, 3731, 4482
Bse8I GATNNNNATC 2 cut(s) 507, 1722
BseAI TCCGGA 1 cut(s) 490
BseBI CCWGG 7 cut(s) 95, 226, 969, 1439, 1969, 2475, 3461
BseCI ATCGAT 1 cut(s) 1145
BseGI GGATG 8 cut(s) 139, 156, 1368, 2693, 3661, 4186, 4926, 5143
BseJI GATNNNNATC 2 cut(s) 507, 1722
BseLI CCNNNNNNNGG 5 cut(s) 190, 991, 2129, 4279, 4936
BseMI GCAATG 4 cut(s) 478, 2719, 3731, 4482
BseMII CTCAG 6 cut(s) 627, 1451, 3081, 3692, 4565, 4706
BseRI GAGGAG 2 cut(s) 769, 1055
BseSI GKGCMC 2 cut(s) 4638, 5024
BseXI GCAGC 9 cut(s) 625, 628, 843, 2197, 2291, 2674, 3182, 3437, 4572
BsgI GTGCAG 6 cut(s) 390, 850, 1993, 2319, 4173, 5022
Bsh1236I CGCG 4 cut(s) 242, 354, 681, 1133
BshFI GGCC 4 cut(s) 1339, 2485, 3841, 4104
BshNI GGYRCC 1 cut(s) 4545
BshVI ATCGAT 1 cut(s) 1145
BsiHKAI GWGCWC 3 cut(s) 21, 4638, 5024
BsiHKCI CYCGRG 1 cut(s) 3041
BsiSI CCGG 6 cut(s) 491, 546, 1340, 1770, 2834, 4446
BsiWI CGTACG 1 cut(s) 3882
BslFI GGGAC 5 cut(s) 1531, 1846, 2765, 2800, 4916
BslI CCNNNNNNNGG 5 cut(s) 190, 991, 2129, 4279, 4936
BsmBI CGTCTC 2 cut(s) 2413, 4808
BsmFI GGGAC 5 cut(s) 1531, 1846, 2765, 2800, 4916
BsmI GAATGC 8 cut(s) 169, 627, 2357, 3071, 3198, 3453, 3513, 3746
BsnI GGCC 4 cut(s) 1339, 2485, 3841, 4104
Bso31I GGTCTC 1 cut(s) 2835
BsoBI CYCGRG 1 cut(s) 3041
Bsp119I TTCGAA 3 cut(s) 2937, 4373, 4515
Bsp1286I GDGCHC 6 cut(s) 21, 250, 459, 1287, 4638, 5024
Bsp13I TCCGGA 1 cut(s) 490
Bsp1407I TGTACA 4 cut(s) 463, 2234, 3945, 4596
Bsp19I CCATGG 1 cut(s) 3812
BspANI GGCC 4 cut(s) 1339, 2485, 3841, 4104
BspCNI CTCAG 6 cut(s) 628, 1452, 3082, 3693, 4564, 4707
BspDI ATCGAT 1 cut(s) 1145
BspEI TCCGGA 1 cut(s) 490
BspFNI CGCG 4 cut(s) 242, 354, 681, 1133
BspHI TCATGA 2 cut(s) 3604, 4027
BspMAI CTGCAG 4 cut(s) 799, 2215, 2281, 5124
BspMI ACCTGC 3 cut(s) 1774, 2311, 5044
BspOI GCTAGC 1 cut(s) 3731
BspPI GGATC 7 cut(s) 191, 689, 702, 1380, 1908, 3937, 4263
BspQI GCTCTTC 1 cut(s) 447
BspT104I TTCGAA 3 cut(s) 2937, 4373, 4515
BspT107I GGYRCC 1 cut(s) 4545
BspTNI GGTCTC 1 cut(s) 2835
BsrBI CCGCTC 1 cut(s) 1927
BsrDI GCAATG 4 cut(s) 478, 2719, 3731, 4482
BsrGI TGTACA 4 cut(s) 463, 2234, 3945, 4596
BssNI GRCGYC 3 cut(s) 8, 1551, 3312
BssSI CACGAG 1 cut(s) 1317
BssT1I CCWWGG 8 cut(s) 698, 1468, 2254, 2818, 3484, 3812, 4685, 4786
Bst2BI CACGAG 1 cut(s) 1317
Bst2UI CCWGG 7 cut(s) 95, 226, 969, 1439, 1969, 2475, 3461
Bst6I CTCTTC 5 cut(s) 447, 897, 1405, 3620, 4763
BstACI GRCGYC 3 cut(s) 8, 1551, 3312
BstAPI GCANNNNNTGC 1 cut(s) 4664
BstAUI TGTACA 4 cut(s) 463, 2234, 3945, 4596
BstBAI YACGTR 2 cut(s) 3886, 4889
BstBI TTCGAA 3 cut(s) 2937, 4373, 4515
BstDEI CTNAG 8 cut(s) 129, 636, 1460, 3090, 3701, 4551, 4581, 4715
BstDSI CCRYGG 2 cut(s) 240, 3812
BstF5I GGATG 8 cut(s) 139, 156, 1368, 2693, 3661, 4186, 4926, 5143
BstFNI CGCG 4 cut(s) 242, 354, 681, 1133
BstHHI GCGC 5 cut(s) 354, 1135, 2392, 3477, 3727
BstMWI GCNNNNNNNGC 9 cut(s) 16, 245, 477, 1130, 2491, 3456, 3733, 4234, 4664
BstNI CCWGG 7 cut(s) 95, 226, 969, 1439, 1969, 2475, 3461
BstNSI RCATGY 4 cut(s) 966, 2655, 3073, 3853
BstSCI CCNGG 7 cut(s) 93, 224, 967, 1437, 1967, 2473, 3459
BstSFI CTRYAG 7 cut(s) 795, 2211, 2277, 2782, 3265, 3835, 5120
BstSLI GKGCMC 2 cut(s) 4638, 5024
BstSNI TACGTA 1 cut(s) 3886
BstUI CGCG 4 cut(s) 242, 354, 681, 1133
BstV1I GCAGC 9 cut(s) 625, 628, 843, 2197, 2291, 2674, 3182, 3437, 4572
BstV2I GAAGAC 7 cut(s) 690, 1554, 3137, 3212, 3548, 3827, 4515
BstX2I RGATCY 7 cut(s) 694, 1900, 3439, 3497, 3929, 4110, 5124
BstXI CCANNNNNNTGG 1 cut(s) 4756
BstYI RGATCY 7 cut(s) 694, 1900, 3439, 3497, 3929, 4110, 5124
Bsu15I ATCGAT 1 cut(s) 1145
Bsu36I CCTNAGG 1 cut(s) 636
BsuI GTATCC 2 cut(s) 1720, 5008
BsuRI GGCC 4 cut(s) 1339, 2485, 3841, 4104
BsuTUI ATCGAT 1 cut(s) 1145
BtgI CCRYGG 2 cut(s) 240, 3812
BtsCI GGATG 8 cut(s) 139, 156, 1368, 2693, 3661, 4186, 4926, 5143
BtsI GCAGTG 3 cut(s) 322, 402, 466
BveI ACCTGC 3 cut(s) 1774, 2311, 5044
CaiI CAGNNNCTG 1 cut(s) 1192
CciI TCATGA 2 cut(s) 3604, 4027
CfoI GCGC 5 cut(s) 354, 1135, 2392, 3477, 3727
Cfr13I GGNCC 2 cut(s) 3902, 4742
Cfr42I CCGCGG 1 cut(s) 243
ClaI ATCGAT 1 cut(s) 1145
CseI GACGC 4 cut(s) 16, 3140, 3320, 4825
CsiI ACCWGGT 1 cut(s) 1437
DdeI CTNAG 8 cut(s) 129, 636, 1460, 3090, 3701, 4551, 4581, 4715
DraI TTTAAA 3 cut(s) 385, 3427, 4389
DriI GACNNNNNGTC 1 cut(s) 1524
EaeI YGGCCR 1 cut(s) 4102
Eam1104I CTCTTC 5 cut(s) 447, 897, 1405, 3620, 4763
Eam1105I GACNNNNNGTC 1 cut(s) 1524
EarI CTCTTC 5 cut(s) 447, 897, 1405, 3620, 4763
EciI GGCGGA 1 cut(s) 4965
Eco105I TACGTA 1 cut(s) 3886
Eco130I CCWWGG 8 cut(s) 698, 1468, 2254, 2818, 3484, 3812, 4685, 4786
Eco147I AGGCCT 1 cut(s) 3841
Eco24I GRGCYC 3 cut(s) 250, 459, 1287
Eco31I GGTCTC 1 cut(s) 2835
Eco32I GATATC 1 cut(s) 2557
Eco47I GGWCC 2 cut(s) 3902, 4742
Eco57I CTGAAG 8 cut(s) 2471, 2809, 3149, 3188, 3431, 3644, 3804, 4481
Eco81I CCTNAGG 1 cut(s) 636
Eco88I CYCGRG 1 cut(s) 3041
EcoRI GAATTC 5 cut(s) 3181, 3647, 4912, 5075, 5152
EcoRII CCWGG 7 cut(s) 93, 224, 967, 1437, 1967, 2473, 3459
EcoRV GATATC 1 cut(s) 2557
EcoT14I CCWWGG 8 cut(s) 698, 1468, 2254, 2818, 3484, 3812, 4685, 4786
EcoT22I ATGCAT 7 cut(s) 1126, 1432, 1511, 2359, 3071, 3075, 3352
EcoT38I GRGCYC 3 cut(s) 250, 459, 1287
ErhI CCWWGG 8 cut(s) 698, 1468, 2254, 2818, 3484, 3812, 4685, 4786
Esp3I CGTCTC 2 cut(s) 2413, 4808
FalI AAGNNNNNCTT 4 cut(s) 1239, 1271, 4549, 4581
FaqI GGGAC 5 cut(s) 1531, 1846, 2765, 2800, 4916
FauI CCCGC 1 cut(s) 235
FbaI TGATCA 7 cut(s) 912, 1450, 1723, 2569, 3232, 4342, 5052
FblI GTMKAC 1 cut(s) 975
FokI GGATG 8 cut(s) 143, 146, 1375, 2680, 3668, 4193, 4913, 5150
FriOI GRGCYC 3 cut(s) 250, 459, 1287
FspI TGCGCA 1 cut(s) 3476
GlaI GCGC 5 cut(s) 353, 1134, 2391, 3476, 3726
GsuI CTGGAG 2 cut(s) 1143, 3699
HaeIII GGCC 4 cut(s) 1339, 2485, 3841, 4104
HapII CCGG 6 cut(s) 491, 546, 1340, 1770, 2834, 4446
HgaI GACGC 4 cut(s) 16, 3140, 3320, 4825
HhaI GCGC 5 cut(s) 354, 1135, 2392, 3477, 3727
Hin1I GRCGYC 3 cut(s) 8, 1551, 3312
Hin6I GCGC 5 cut(s) 352, 1133, 2390, 3475, 3725
HinP1I GCGC 5 cut(s) 352, 1133, 2390, 3475, 3725
HincII GTYRAC 1 cut(s) 4203
HindII GTYRAC 1 cut(s) 4203
HindIII AAGCTT 4 cut(s) 1384, 1931, 4298, 4558
HpaI GTTAAC 1 cut(s) 4203
HpaII CCGG 6 cut(s) 491, 546, 1340, 1770, 2834, 4446
Hpy166II GTNNAC 9 cut(s) 139, 464, 466, 976, 1486, 4203, 4636, 5009, 5022
Hpy8I GTNNAC 9 cut(s) 139, 464, 466, 976, 1486, 4203, 4636, 5009, 5022
Hpy99I CGWCG 4 cut(s) 10, 1993, 2385, 5135
HpyCH4IV ACGT 7 cut(s) 325, 1551, 2184, 2813, 3237, 3885, 4888
HpyF10VI GCNNNNNNNGC 9 cut(s) 16, 245, 477, 1130, 2491, 3456, 3733, 4234, 4664
HpyF3I CTNAG 8 cut(s) 129, 636, 1460, 3090, 3701, 4551, 4581, 4715
HpySE526I ACGT 7 cut(s) 325, 1551, 2184, 2813, 3237, 3885, 4888
Hsp92I GRCGYC 3 cut(s) 8, 1551, 3312
HspAI GCGC 5 cut(s) 352, 1133, 2390, 3475, 3725
Kpn2I TCCGGA 1 cut(s) 490
KpnI GGTACC 1 cut(s) 4549
Ksp22I TGATCA 7 cut(s) 912, 1450, 1723, 2569, 3232, 4342, 5052
KspAI GTTAAC 1 cut(s) 4203
KspI CCGCGG 1 cut(s) 243
LguI GCTCTTC 1 cut(s) 447
LmnI GCTCC 9 cut(s) 253, 1162, 1294, 1561, 2311, 2611, 4084, 4448, 5047
Lsp1109I GCAGC 9 cut(s) 625, 628, 843, 2197, 2291, 2674, 3182, 3437, 4572
MabI ACCWGGT 1 cut(s) 1437
MaeII ACGT 7 cut(s) 325, 1551, 2184, 2813, 3237, 3885, 4888
MbiI CCGCTC 1 cut(s) 1927
MfeI CAATTG 1 cut(s) 930
MflI RGATCY 7 cut(s) 694, 1900, 3439, 3497, 3929, 4110, 5124
MhlI GDGCHC 6 cut(s) 21, 250, 459, 1287, 4638, 5024
MlyI GAGTC 4 cut(s) 314, 2519, 2637, 3669
MmeI TCCRAC 5 cut(s) 301, 584, 2832, 3210, 4488
Mph1103I ATGCAT 7 cut(s) 1126, 1432, 1511, 2359, 3071, 3075, 3352
MroI TCCGGA 1 cut(s) 490
MroXI GAANNNNTTC 1 cut(s) 2928
MslI CAYNNNNRTG 3 cut(s) 471, 2397, 4754
MspA1I CMGCKG 1 cut(s) 242
MspI CCGG 6 cut(s) 491, 546, 1340, 1770, 2834, 4446
MspR9I CCNGG 7 cut(s) 95, 226, 969, 1439, 1969, 2475, 3461
MunI CAATTG 1 cut(s) 930
Mva1269I GAATGC 8 cut(s) 169, 627, 2357, 3071, 3198, 3453, 3513, 3746
MvaI CCWGG 7 cut(s) 95, 226, 969, 1439, 1969, 2475, 3461
MvnI CGCG 4 cut(s) 242, 354, 681, 1133
MwoI GCNNNNNNNGC 9 cut(s) 16, 245, 477, 1130, 2491, 3456, 3733, 4234, 4664
NcoI CCATGG 1 cut(s) 3812
NheI GCTAGC 1 cut(s) 3727
NmeAIII GCCGAG 1 cut(s) 36
NmuCI GTSAC 5 cut(s) 748, 1000, 3012, 3973, 4335
NsbI TGCGCA 1 cut(s) 3476
NsiI ATGCAT 7 cut(s) 1126, 1432, 1511, 2359, 3071, 3075, 3352
NspI RCATGY 4 cut(s) 966, 2655, 3073, 3853
NspV TTCGAA 3 cut(s) 2937, 4373, 4515
PaeI GCATGC 2 cut(s) 2655, 3073
PagI TCATGA 2 cut(s) 3604, 4027
PceI AGGCCT 1 cut(s) 3841
PciI ACATGT 2 cut(s) 962, 3849
PciSI GCTCTTC 1 cut(s) 447
PctI GAATGC 8 cut(s) 169, 627, 2357, 3071, 3198, 3453, 3513, 3746
PdmI GAANNNNTTC 1 cut(s) 2928
Pfl23II CGTACG 1 cut(s) 3882
PflMI CCANNNNNTGG 2 cut(s) 4279, 4936
PleI GAGTC 4 cut(s) 313, 2518, 2636, 3668
PpsI GAGTC 4 cut(s) 313, 2518, 2636, 3668
Ppu21I YACGTR 2 cut(s) 3886, 4889
PscI ACATGT 2 cut(s) 962, 3849
PshBI ATTAAT 2 cut(s) 1392, 2595
Psp6I CCWGG 7 cut(s) 93, 224, 967, 1437, 1967, 2473, 3459
PspGI CCWGG 7 cut(s) 93, 224, 967, 1437, 1967, 2473, 3459
PspLI CGTACG 1 cut(s) 3882
PspPI GGNCC 2 cut(s) 3902, 4742
PstI CTGCAG 4 cut(s) 799, 2215, 2281, 5124
PstNI CAGNNNCTG 1 cut(s) 1192
PsuI RGATCY 7 cut(s) 694, 1900, 3439, 3497, 3929, 4110, 5124
RseI CAYNNNNRTG 3 cut(s) 471, 2397, 4754
SacII CCGCGG 1 cut(s) 243
SapI GCTCTTC 1 cut(s) 447
Sau96I GGNCC 2 cut(s) 3902, 4742
SchI GAGTC 4 cut(s) 314, 2519, 2637, 3669
ScrFI CCNGG 7 cut(s) 95, 226, 969, 1439, 1969, 2475, 3461
SduI GDGCHC 6 cut(s) 21, 250, 459, 1287, 4638, 5024
SexAI ACCWGGT 1 cut(s) 1437
SfcI CTRYAG 7 cut(s) 795, 2211, 2277, 2782, 3265, 3835, 5120
Sfr303I CCGCGG 1 cut(s) 243
SfuI TTCGAA 3 cut(s) 2937, 4373, 4515
SgrBI CCGCGG 1 cut(s) 243
SinI GGWCC 2 cut(s) 3902, 4742
SmiMI CAYNNNNRTG 3 cut(s) 471, 2397, 4754
SmlI CTYRAG 5 cut(s) 206, 2507, 2948, 3443, 4066
SmoI CTYRAG 5 cut(s) 206, 2507, 2948, 3443, 4066
SnaBI TACGTA 1 cut(s) 3886
SphI GCATGC 2 cut(s) 2655, 3073
SseBI AGGCCT 1 cut(s) 3841
SspI AATATT 3 cut(s) 1354, 2744, 3335
StuI AGGCCT 1 cut(s) 3841
StyD4I CCNGG 7 cut(s) 93, 224, 967, 1437, 1967, 2473, 3459
StyI CCWWGG 8 cut(s) 698, 1468, 2254, 2818, 3484, 3812, 4685, 4786
TaiI ACGT 7 cut(s) 328, 1554, 2187, 2816, 3240, 3888, 4891
TaqII GACCGA 1 cut(s) 1514
TatI WGTACW 7 cut(s) 463, 2234, 2601, 2716, 3945, 4596, 5008
TauI GCSGC 4 cut(s) 242, 670, 1671, 2026
TseFI GTSAC 5 cut(s) 748, 1000, 3012, 3973, 4335
TseI GCWGC 9 cut(s) 613, 616, 831, 2210, 2279, 2687, 3195, 3450, 4585
Tsp45I GTSAC 5 cut(s) 748, 1000, 3012, 3973, 4335
TspGWI ACGGA 6 cut(s) 1007, 2261, 2300, 2533, 2720, 4409
Van91I CCANNNNNTGG 2 cut(s) 4279, 4936
VneI GTGCAC 2 cut(s) 4634, 5020
VpaK11BI GGWCC 2 cut(s) 3902, 4742
VspI ATTAAT 2 cut(s) 1392, 2595
XceI RCATGY 4 cut(s) 966, 2655, 3073, 3853
XmaJI CCTAGG 1 cut(s) 2254
XmiI GTMKAC 1 cut(s) 975
XmnI GAANNNNTTC 1 cut(s) 2928
ZraI GACGTC 1 cut(s) 1552
Zsp2I ATGCAT 7 cut(s) 1126, 1432, 1511, 2359, 3071, 3075, 3352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.