RLG00000004622

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000526
Physical Location & Seq
Reverse (-)
60871608 .. 60877865
6258 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004622

Sequence Viewer

Length: 5103 bp
ATGGCGACGACGATGACGCCAAGGGAACACATAGAAGATATACGGAAGACCAAATTCTCCATCGGAGAAAAGCTCAACCCTTTAACGGAGGATCTTCACCAGGCCGTCAAGAACCTCTCCGGCGAGCTTTACGCTAAAGACGTCCACTTTTTCATGGAACTCGTTCAGAATGCAGAAGACAATGAGTACTTGGAGGGGGTTGATCCATCCCTTGAGTTTGTGATTACATACCTCGATATAACGGCTACAGGGGCTCCAGCGACATTGCTGGTTTTCAATAATGAGAAGGGGTTTTCTCCCAGAAACATAGACTCCATTTGCAGTGTTGGACGGTCCACCAAGAAAGGCAACAGGAAGCGTGGTTATATTGGGGAGAAAGGAATTGGCTTCAAGAGTGTGTTTCTGATCACTGCTCAGCCTTACATATTTAGTAATGGTTATCAGATAAGGTTTAGTGAAGAGCCTTGTTCACATTGCAATGTTGGCTACATCGTACCTGAATGGGTTGAGCAGAATCCCACTCTATCTGATATAGAAAAGATATATGGTTCTGCTACTGCCCTTCCAACCACAACACTGATCTTACCTCTGAAGCCCGACAAGGTTGAACCTGTGAAGAAACAACTCTCGATCATTCATCCAGAAGTTCTCTTGTTCCTTTCAAAGATTAAGCGGCTCTCTGTTAGGGAAGACAAAGAGGACCCAAGGCAGAACACTGTGAGTGCAATTGAGATAGCAAGCAAGACTGAATTTGTGTCAAGGAAGAACATCGATGCTCAATCCTACACTCTGCATCTTTCAGCTGAAGAAAATGATGGAAATGGAGAATGCAGTTACTACATGTGGAAACAAAAGTTTCCTGTCAGGCAGGAATTCAGAGTTGAGAGGAGAATGGAGGTGGATGAGTGGGTGATCACTCTTGCATTTCCTAATGGAGAACGTCTTCAAAGAGCAAGGAACTCTTCACCTGGTGTTTATGCATTTCTTCCCACAGAGATGTTCACCAACTTTCCATTCATAATACAGGCGGATTTTCTTTTAGCATCTTCAAGAGAGACAATCCTACTTGACAACAAGTGGAATCAAGGGATTCTTGAGTGTGTGCCCACCGCATTTGCCCATGCCTTTAACTCTCTTGTGAAAACTGTGGAAGATGCGCCAGCATCAAGTTTGGTTCCTATGTTCAGATTCTTGCCTGTTCAGGGTTCTCCCTATAAAGAATTAAATACTGTTAGGGAGTCGATAAAAGAGAAGATGGTTGAGGAAGACATTATACCAGTTGAGCCCGAAATGGAGCAAAAGTTCTTCCACAAGCCTCGTGAAGTGGGAAGATTAATGCCTGCCTTTTGGTCTATTTTGAGGAAGGCGAGGGTTCAAGGTGTAAGCTTGATTAAGCTTTCATCACATGGAAGATTTGTTCTGTCTTATTCCTTTGACAGCAAGGAATATGATCCTATACTAAGTTTCCTTGGAGTTGAACCAGTGAATGATGACTGGTATGCTAAATGCATACAGGGTACTTCTAATCTTGTTGGTGGAGTGTCAGAAGATCTGTACTTAGAGCTTCTACAGTTTATCGCTGAAAATTGGGGTTCTAAATTTGGTCACACCAACATGAAGAACATACCATTGATAAAATATGCAGACTATGGAAATGAATCTTTGTGCAGCATAAGCGAAATCCAGAAAGGTGAATGTAGAGTTTGCTTATCGAGCAAATATTCTCGTGTTTCATGGCTGATTGATTGCAACCGGGAATTTAATTCTGTGACTCGTCTTCTCTTTATGCCGAAAGCCACTCAGGAAGCCATCTGGACATGCTCTGGGAAGGATACATTGAAGAAATGGCTAGCTAATCAAGCTAAGGTTGGTTCTGTTGATGTCAATCAATATGCAGATCTCCTTCAGAATTCTCTTAATGAGCGGAAGCTCGTTATTGCATATGTTCATTTCTTGTATCACTCGGTTGATAATGATTTTATCTCAAGTCGGGAAGTTGACCGTTTATGTGGAATTATGCCACTAATGGATGGCTACGGGGACATCGTCAGAAGTAGAAAAGGGGTTATTGTTCCTGCCAATGGAAGCAAATGGGCGGGATTGACTGATTCTAATTTGTGGAGAAAAGAAGGCTATGTTGAATTAGGAGAGCATTATATGAATGCTGGCTATTTTGCTGGTAAGTATACACCGCGGAAAAAGCTTCTTGAGTTCCTTGTAGAACATGCTGCTGCTATGGATGTCCCTCATATATCTGCTCCAAGTGATGGTATCTCGTCTGTATCTGCACCACTAACTAAGCAGAATGCCTTCTTGCTTTTGGATTGGATTTGTCAACTGAATTATAAAAGGGTTTGCATCCCTCAGAAGTTCTTGGCTTGCATAAAGGAAGGTAGTTGGCTGAAAGTTACTTTGAATGGCTCTCCTGGTTATAGGCCACCATCCCAGTCATTTCTGCTTAACTCATCATGGGGAAATACTTTGCAGAATGGATCCGTCTCTGTTGACATTCCATTGATTGATACGAGCTTTTATGGTGACAGCATTAGTGAATACAAGGAGGAGCTAAAGACAATTGGGGTCATGTTTGAATATAGTGAAGCATGTGACTTCATTGGGAAGCATTTCATGTCTCTGGCAGCTTCATCCTCTTTAACTAGAGGAAATGTGCTTTCCATTCTGCGTTTCATCAGGTTTTTAAGGGATAAACTTCTTCCTCCGGCCGATTTTATCTCTGCTATTAAGAAGGGGAACTGGCTGAAGACTTCCGTAGGCTACAGGTCTCCTGTAGGATCTGTTTTGTCAGATAAGGAGTGGAATGTTGCCTCCAAAATTAGCAACATTCCCTTCATAGATCAGGACTTTTACGGTGATGAAATCTGTAATTTCAGAACTGAACTTGAGTTGCTCGGTGTTGTAATCTCCTTCAACAGAAGTTACCAACTTATAATTGACAACTTGAAGTCACCATCTTCCATAACTTCTTGGACACCTCAGGTTGTCATCTTAATGCTCAACTGTATGGCTCTATCCAAGTCATCTGATAAACTTGTCAATGCATTGAAAGGAGCCAAGTGCCTGAAGACAAGTGTTGGTTATAAGTGTCCAGGGGAATGTCTCTTGTTTCATCACGAATGGGGTTGCATTCTTCAGGTCGTCAATGGTCTTCCAGTGATTGATCATGAATTCTATAGGAGCAACATTTTCCGGTACATAAATGAACTGAAAAAAATTGGTGTGGTGGTTGATTTTGAAGGGGCTGCAAAAGTATTTGCTCGATATTTCAGGCACTATGCATCATCAGATTCCATCACCAAGGAAAATGTTGCATCATTGCTTCTGTGTTACAGAGCGCTGAAGGGGACTGCATATAAATTTCCTGCTGATCTTAGGAGCTGCATTCGTGAGGTCAAGTGGTTGCGTAGTCGTTTTGGTGATTTCAGATGTCCAAGGGAGTGCATTCTCTTTAGTCCCGAGTGGGAATCTATATCCCCAATAACTATGCTTCCTTTCATTGATGATTCTGACAGTTACTATGGAAAGAGCATACATGAGTACAGAAAAGAGCTGAAGAGCTTGGGGGTTGTCGTCGAATACAAGGAAGGAGTTGAGTTTGTGGCTTCTGGTCTTTGTTTCCCTGGGGATGCCAGCCGTATTTTTCCTGTAAATGCACTTGCGTTGCTGGAATGCATACGAGTTTTCCAGGAGAAAGGTTACGCCTTTCTTGATAATTCTCATTTCATGAAGAAAATTTCTCAACCATGGATAAAGACTCATGCTGGTTATAGGTCTCCCAAAGAGTCTTTGTTGTTTGATTCCAAGTTTGGCTTATATTTGAAGCGGACTGATGGGCCTTTCATTGATGAAGAATTCTATGGTACTAAGATTGCATCTTACAAAAAAGAGCTGTCTGCATTGGGGGTTATTGTTGAAGCTGGAAAGGGGTGTCCCTTGATTGCCGGCCACCTTTATATTCATAATGAGACTGAAGCTTTTGTTCGATTGTATAGTTACTTGAGTGAGTTCCAGTGGGAGCCAGACACTGATGCTGCTCGAAGGATCTGGATTCCAAAAGGGGAGTGGGTTTGTCCTGATGAATGTGTTATTTATGACAAGGATGGGCTATTTGGTTTGCAGTTGACTGTTTTGGAGAAACATTATGAGCATAAACTTCTGCTTTTCTTCTCCAGTGCTTTTAGAGTCAAATCCAATCCTACAGTTGACGACTACTTGAGGATTTGGAAGGTTTGGGAAAGTTCTGGAAGTGCATTGTCGCATGCTGATTGTTGCAAGTATTGGAGTTATATCTCGAAGCACTGGAATTCAAAGACAGAAAAGACTGTTGCTGATGCCTTGGTGAAAGTACCTGTCAGTTCTGGATCTGAAGGAATCTTGCTGTGCAACAAGTCTGATGTTTTCATTGCTGATGACCTTCAACTGAAGTATCTATTCGAACAGTCTTCTCCCGAGTCTATTTTTGTTTGGTATCCTCAGCCAAGTTTGCCTTCCATTCCAAGATCCAAGTTGCTTGACATCTATCAAAAAATTGGTGTTTGCACTATATCTGAATCTGTACAGAAGGAAGAGGCGTCACTTGCAGATGATGTTGAAGTAGTCTCTTTGCCGAGGGAAAAGCTGATTAAGAAAGCGCTGTTCAGGCTGATTCTTGGTTTTCTTGCTGCTCCTCCCATGGAAATGGAAGCTGAGAAGAGGCTTGAAGCTGTTCAATTCCTTGTGAATGTTACTGTGGTGGTAACAGCTGAACCAATCTCTGTAAGCTATAATCTACCTTTATCTTCTGGGAAAGTTTTGAATGTGAGAGGAAGCCGAAAAATACGGTTTGATAGAGAAAATTCAAAGATCTTCACTCAGAAAGTGGACAAGTCTGGAGGGCAGAAGAGTATCATTGAGTTTGCTACTTTTTTCTCTGAAGCGATATCTGAGAGTGTGTTATGGGAGAGCACTGATCATATAGATTCACTCTCTGAGCTGATCAAGATGGCTTCTTTGCTAGGCTTCAATGAGGAAGCAGTTGATTTCTTGATGAAATCCAAGAATTTGCAAACTTTTATGGAGGATGAGGAGTTTCTCAAGACTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1701

Amino Acids

192.72

Weight (kDa)

6.33

Isoelectric Point (pI)

40.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 30 - 146 2.2e-10 Sacsin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28020 AT3G48770 AT3G48770
fragaria_vesca FvH4_5g09020 FvH4_5g09020
malus_domestica MD06G1092400.v1.1 MD06G1134600.v1.1 MD06G1134700.v1.1 MD06G1134800.v1.1 MD06G1135200.v1.1 MD06G1135400.v1.1 MD06G1135500.v1.1 MD08G1137600.v1.1 MD15G1395100.v1.1
prunus_persica Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145600_v2.0.a1 Prupe.5G145800_v2.0.a1 Prupe.5G145900_v2.0.a1
pyrus_communis pycom06g12550 pycom06g12570 pycom06g12580 pycom06g12590 pycom06g12610 pycom06g12630 pycom06g12640 pycom06g12650 pycom08g06280
rosa_chinensis RchiOBHm_Chr6g0249321 RchiOBHm_Chr6g0275111 RchiOBHm_Chr7g0189191 RchiOBHm_Chr7g0189261
rosa_laevigata RLG00000004622 RLG00000004623 RLG00000008237 RLG00000008238 RLG00000023303 RLG00000035187
rosa_multiflora Rmu_co8294311.1_g000001 Rmu_co8389833.1_g000001 Rmu_sc0001044.1_g000004 Rmu_sc0001329.1_g000011 Rmu_sc0006012.1_g000010 Rmu_sc0007286.1_g000032 Rmu_sc0009360.1_g000016 Rmu_sc0011817.1_g000001 Rmu_sc0015050.1_g000001
rosa_roxburghii Rroxscaffold_1G00064000 Rroxscaffold_3G00265460 Rroxscaffold_3G00265580 Rroxscaffold_3G00265640 Rroxscaffold_3G00265690 Rroxscaffold_3G00265700 Rroxscaffold_7G00193620 Rroxscaffold_7G00204550 Rroxscaffold_7G00217350
rosa_rugosa Rorug01G0465500 Rorug06G0088500 Rorug06G0496700 Rorug06G0496700
rosa_samantha Rh1AG064800 Rh2BG015100 Rh2BG493500 Rh2DG017300 Rh2DG502500 Rh4BG208500 Rh5BG528500 Rh5CG552900 Rh6CG207800 Rh6DG197900 Rh6DG198000 Rh7AG101300 Rh7AG101400 Rh7AG101600 Rh7BG104100 Rh7BG104200 Rh7CG105600 Rh7CG105700 Rh7DG103200 Rh7DG103400
rosa_wichuraiana Rw1G007300 Rw5G034670 Rw7G008750 Rw7G008780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2346, 2948, 3099
AatII GACGTC 1 cut(s) 144
AccB7I CCANNNNNTGG 1 cut(s) 2267
AccBSI CCGCTC 1 cut(s) 1924
AccI GTMKAC 1 cut(s) 2186
AccII CGCG 1 cut(s) 2194
AciI CCGC 8 cut(s) 673, 1028, 1110, 1924, 2096, 2192, 2194, 3841
AclWI GGATC 9 cut(s) 99, 197, 1445, 2487, 2500, 2800, 4067, 4387, 4512
AcoI YGGCCR 2 cut(s) 2721, 3961
AcyI GRCGYC 3 cut(s) 17, 141, 4589
AdeI CACNNNGTG 1 cut(s) 971
AfaI GTAC 9 cut(s) 188, 495, 1519, 1556, 3212, 3557, 3880, 4365, 4575
AfeI AGCGCT 2 cut(s) 3354, 4650
AfiI CCNNNNNNNGG 4 cut(s) 85, 1202, 2081, 2267
AflIII ACRYGT 1 cut(s) 840
AjnI CCWGG 6 cut(s) 99, 967, 2425, 3106, 3637, 3702
AjuI GAANNNNNNNTTGG 4 cut(s) 998, 1030, 4489, 4521
AloI GAACNNNNNNTCC 4 cut(s) 1286, 1318, 1401, 1433
Alw21I GWGCWC 1 cut(s) 4964
Alw26I GTCTC 8 cut(s) 1049, 2503, 2637, 2787, 3122, 3795, 3977, 4621
AlwI GGATC 9 cut(s) 99, 197, 1445, 2487, 2500, 2800, 4067, 4387, 4512
AlwNI CAGNNNCTG 1 cut(s) 4043
Ama87I CYCGRG 2 cut(s) 3473, 4466
Aor51HI AGCGCT 2 cut(s) 3354, 4650
AoxI GGCC 5 cut(s) 102, 2435, 2721, 3851, 3961
ApeKI GCWGC 8 cut(s) 1668, 2228, 2231, 2639, 3260, 3396, 4049, 4679
AseI ATTAAT 1 cut(s) 1334
Asp700I GAANNNNTTC 5 cut(s) 162, 942, 2309, 2624, 4722
AspLEI GCGC 3 cut(s) 1159, 3355, 4651
AspS9I GGNCC 3 cut(s) 333, 700, 3851
AsuC2I CCSGG 1 cut(s) 1754
AsuII TTCGAA 1 cut(s) 4452
AsuNHI GCTAGC 1 cut(s) 1849
AvaI CYCGRG 2 cut(s) 3473, 4466
AvaII GGWCC 2 cut(s) 333, 700
AxyI CCTNAGG 1 cut(s) 2994
BaeGI GKGCMC 1 cut(s) 1107
BaeI ACNNNNGTAYC 2 cut(s) 4427, 4460
BamHI GGATCC 1 cut(s) 2492
BanII GRGCYC 2 cut(s) 256, 1287
BarI GAAGNNNNNNTAC 2 cut(s) 1257, 1289
BauI CACGAG 2 cut(s) 1317, 1725
Bbv12I GWGCWC 1 cut(s) 4964
BbvCI CCTCAGC 1 cut(s) 4491
BbvI GCAGC 8 cut(s) 1680, 2215, 2218, 2651, 3247, 3383, 4036, 4666
BceAI ACGGC 3 cut(s) 89, 258, 3636
BciT130I CCWGG 6 cut(s) 101, 969, 2427, 3108, 3639, 3704
BciVI GTATCC 2 cut(s) 1825, 4497
BclI TGATCA 5 cut(s) 405, 912, 3178, 4966, 4992
BcnI CCSGG 1 cut(s) 1754
BcoDI GTCTC 8 cut(s) 1049, 2503, 2637, 2787, 3122, 3795, 3977, 4621
BfaI CTAG 4 cut(s) 1850, 2658, 5012, 5101
BfmI CTRYAG 6 cut(s) 246, 1568, 2776, 2787, 3190, 4215
BfoI RGCGCY 2 cut(s) 3356, 4652
BfuI GTATCC 2 cut(s) 1825, 4497
BglII AGATCT 3 cut(s) 1549, 1897, 4860
BisI GCNGC 9 cut(s) 674, 1669, 2229, 2232, 2640, 3261, 3397, 4050, 4680
BlpI GCTNAGC 1 cut(s) 414
BlsI GCNGC 9 cut(s) 675, 1670, 2230, 2233, 2641, 3262, 3398, 4051, 4681
BmcAI AGTACT 1 cut(s) 188
Bme1390I CCNGG 7 cut(s) 101, 969, 1754, 2427, 3108, 3639, 3704
Bme18I GGWCC 2 cut(s) 333, 700
BmeT110I CYCGRG 2 cut(s) 3473, 4466
BmgT120I GGNCC 3 cut(s) 333, 700, 3851
BmiI GGNNCC 6 cut(s) 255, 702, 1176, 2494, 3070, 4035
BmrFI CCNGG 7 cut(s) 101, 969, 1754, 2427, 3108, 3639, 3704
BmrI ACTGGG 1 cut(s) 2440
BmtI GCTAGC 1 cut(s) 1853
BmuI ACTGGG 1 cut(s) 2440
BplI GAGNNNNNCTC 2 cut(s) 57, 89
BpmI CTGGAG 3 cut(s) 240, 4171, 4908
Bpu10I CCTNAGC 2 cut(s) 1863, 4491
Bpu1102I GCTNAGC 1 cut(s) 414
Bpu14I TTCGAA 1 cut(s) 4452
BpuEI CTTGAG 8 cut(s) 233, 1115, 1969, 2228, 2921, 4036, 4252, 5075
BpuMI CCSGG 1 cut(s) 1754
Bsa29I ATCGAT 1 cut(s) 771
BsaBI GATNNNNATC 1 cut(s) 1884
BsaHI GRCGYC 3 cut(s) 17, 141, 4589
BsaI GGTCTC 2 cut(s) 2787, 3795
BsaWI WCCGGW 1 cut(s) 3207
BsaXI ACNNNNNCTCC 6 cut(s) 238, 268, 296, 326, 1286, 1316
Bsc4I CCNNNNNNNGG 4 cut(s) 85, 1202, 2081, 2267
Bse118I RCCGGY 1 cut(s) 3959
Bse1I ACTGG 9 cut(s) 1277, 1481, 1499, 2446, 2759, 3170, 4027, 4188, 4322
Bse21I CCTNAGG 1 cut(s) 2994
Bse3DI GCAATG 5 cut(s) 263, 472, 484, 3332, 4419
Bse8I GATNNNNATC 1 cut(s) 1884
BseBI CCWGG 6 cut(s) 101, 969, 2427, 3108, 3639, 3704
BseCI ATCGAT 1 cut(s) 771
BseJI GATNNNNATC 1 cut(s) 1884
BseLI CCNNNNNNNGG 4 cut(s) 85, 1202, 2081, 2267
BseMI GCAATG 5 cut(s) 263, 472, 484, 3332, 4419
BseMII CTCAG 9 cut(s) 428, 1814, 2378, 3008, 4505, 4695, 4883, 4932, 4977
BseNI ACTGG 9 cut(s) 1277, 1481, 1499, 2446, 2759, 3170, 4027, 4188, 4322
BseRI GAGGAG 4 cut(s) 901, 2576, 4674, 5096
BseSI GKGCMC 1 cut(s) 1107
BseX3I CGGCCG 1 cut(s) 2721
BseXI GCAGC 8 cut(s) 1680, 2215, 2218, 2651, 3247, 3383, 4036, 4666
BsgI GTGCAG 2 cut(s) 1687, 2271
Bsh1236I CGCG 1 cut(s) 2194
Bsh1285I CGRYCG 1 cut(s) 2724
BshFI GGCC 5 cut(s) 104, 2437, 2723, 3853, 3963
BshVI ATCGAT 1 cut(s) 771
BsiEI CGRYCG 1 cut(s) 2724
BsiHKAI GWGCWC 1 cut(s) 4964
BsiHKCI CYCGRG 2 cut(s) 3473, 4466
BsiSI CCGG 5 cut(s) 120, 1753, 2720, 3208, 3960
BslFI GGGAC 5 cut(s) 2054, 2228, 3376, 3456, 3933
BslI CCNNNNNNNGG 4 cut(s) 85, 1202, 2081, 2267
BsmAI GTCTC 8 cut(s) 1049, 2503, 2637, 2787, 3122, 3795, 3977, 4621
BsmBI CGTCTC 1 cut(s) 2503
BsmFI GGGAC 5 cut(s) 2054, 2228, 3376, 3456, 3933
BsmI GAATGC 8 cut(s) 175, 833, 2167, 2311, 3144, 3399, 3459, 3692
BsnI GGCC 5 cut(s) 104, 2437, 2723, 3853, 3963
Bso31I GGTCTC 2 cut(s) 2787, 3795
BsoBI CYCGRG 2 cut(s) 3473, 4466
Bsp119I TTCGAA 1 cut(s) 4452
Bsp1286I GDGCHC 4 cut(s) 256, 1107, 1287, 4964
Bsp1407I TGTACA 1 cut(s) 4573
Bsp1720I GCTNAGC 1 cut(s) 414
Bsp19I CCATGG 2 cut(s) 3761, 4689
BspACI CCGC 8 cut(s) 673, 1028, 1110, 1924, 2096, 2192, 2194, 3841
BspANI GGCC 5 cut(s) 104, 2437, 2723, 3853, 3963
BspCNI CTCAG 9 cut(s) 427, 1813, 2377, 3007, 4504, 4696, 4882, 4933, 4978
BspDI ATCGAT 1 cut(s) 771
BspFNI CGCG 1 cut(s) 2194
BspHI TCATGA 2 cut(s) 3181, 3741
BspLI GGNNCC 6 cut(s) 255, 702, 1176, 2494, 3070, 4035
BspOI GCTAGC 1 cut(s) 1853
BspPI GGATC 9 cut(s) 99, 197, 1445, 2487, 2500, 2800, 4067, 4387, 4512
BspQI GCTCTTC 2 cut(s) 453, 3566
BspT104I TTCGAA 1 cut(s) 4452
BspTNI GGTCTC 2 cut(s) 2787, 3795
BsrBI CCGCTC 1 cut(s) 1924
BsrDI GCAATG 5 cut(s) 263, 472, 484, 3332, 4419
BsrFI RCCGGY 1 cut(s) 3959
BsrGI TGTACA 1 cut(s) 4573
BsrI ACTGG 9 cut(s) 1277, 1481, 1499, 2446, 2759, 3170, 4027, 4188, 4322
BssAI RCCGGY 1 cut(s) 3959
BssNAI GTATAC 1 cut(s) 2187
BssNI GRCGYC 3 cut(s) 17, 141, 4589
BssSI CACGAG 2 cut(s) 1317, 1725
BssT1I CCWWGG 8 cut(s) 20, 704, 1468, 3315, 3449, 3761, 4353, 4689
Bst1107I GTATAC 1 cut(s) 2187
Bst2BI CACGAG 2 cut(s) 1317, 1725
Bst2UI CCWGG 6 cut(s) 101, 969, 2427, 3108, 3639, 3704
Bst6I CTCTTC 6 cut(s) 453, 967, 3566, 4578, 4703, 4892
BstACI GRCGYC 3 cut(s) 17, 141, 4589
BstAUI TGTACA 1 cut(s) 4573
BstBI TTCGAA 1 cut(s) 4452
BstDSI CCRYGG 3 cut(s) 2192, 3761, 4689
BstFNI CGCG 1 cut(s) 2194
BstH2I RGCGCY 2 cut(s) 3356, 4652
BstHHI GCGC 3 cut(s) 1159, 3355, 4651
BstMAI GTCTC 8 cut(s) 1049, 2503, 2637, 2787, 3122, 3795, 3977, 4621
BstMCI CGRYCG 1 cut(s) 2724
BstMWI GCNNNNNNNGC 9 cut(s) 251, 483, 1674, 1713, 1859, 2200, 4501, 4595, 4657
BstNI CCWGG 6 cut(s) 101, 969, 2427, 3108, 3639, 3704
BstNSI RCATGY 5 cut(s) 844, 1821, 2228, 2607, 4280
BstSCI CCNGG 7 cut(s) 99, 967, 1752, 2425, 3106, 3637, 3702
BstSFI CTRYAG 6 cut(s) 246, 1568, 2776, 2787, 3190, 4215
BstSLI GKGCMC 1 cut(s) 1107
BstUI CGCG 1 cut(s) 2194
BstV1I GCAGC 8 cut(s) 1680, 2215, 2218, 2651, 3247, 3383, 4036, 4666
BstX2I RGATCY 9 cut(s) 91, 1549, 1897, 2492, 2792, 4059, 4379, 4517, 4860
BstXI CCANNNNNNTGG 1 cut(s) 4696
BstYI RGATCY 9 cut(s) 91, 1549, 1897, 2492, 2792, 4059, 4379, 4517, 4860
BstZ17I GTATAC 1 cut(s) 2187
BstZI CGGCCG 1 cut(s) 2721
Bsu15I ATCGAT 1 cut(s) 771
Bsu36I CCTNAGG 1 cut(s) 2994
BsuI GTATCC 2 cut(s) 1825, 4497
BsuRI GGCC 5 cut(s) 104, 2437, 2723, 3853, 3963
BsuTUI ATCGAT 1 cut(s) 771
BtgI CCRYGG 3 cut(s) 2192, 3761, 4689
BtsI GCAGTG 2 cut(s) 328, 408
CaiI CAGNNNCTG 1 cut(s) 4043
CciI TCATGA 2 cut(s) 3181, 3741
CfoI GCGC 3 cut(s) 1159, 3355, 4651
Cfr10I RCCGGY 1 cut(s) 3959
Cfr13I GGNCC 3 cut(s) 333, 700, 3851
Cfr42I CCGCGG 1 cut(s) 2195
ClaI ATCGAT 1 cut(s) 771
CseI GACGC 2 cut(s) 25, 4578
CsiI ACCWGGT 1 cut(s) 967
Csp6I GTAC 9 cut(s) 187, 494, 1518, 1555, 3211, 3556, 3879, 4364, 4574
CspCI CAANNNNNGTGG 2 cut(s) 1096, 1131
CviQI GTAC 9 cut(s) 187, 494, 1518, 1555, 3211, 3556, 3879, 4364, 4574
DraIII CACNNNGTG 1 cut(s) 971
EaeI YGGCCR 2 cut(s) 2721, 3961
EagI CGGCCG 1 cut(s) 2721
Eam1104I CTCTTC 6 cut(s) 453, 967, 3566, 4578, 4703, 4892
EarI CTCTTC 6 cut(s) 453, 967, 3566, 4578, 4703, 4892
EciI GGCGGA 1 cut(s) 1043
EclXI CGGCCG 1 cut(s) 2721
Eco130I CCWWGG 8 cut(s) 20, 704, 1468, 3315, 3449, 3761, 4353, 4689
Eco24I GRGCYC 2 cut(s) 256, 1287
Eco31I GGTCTC 2 cut(s) 2787, 3795
Eco32I GATATC 1 cut(s) 4938
Eco47I GGWCC 2 cut(s) 333, 700
Eco47III AGCGCT 2 cut(s) 3354, 4650
Eco52I CGGCCG 1 cut(s) 2721
Eco81I CCTNAGG 1 cut(s) 2994
Eco88I CYCGRG 2 cut(s) 3473, 4466
EcoO109I RGGNCCY 1 cut(s) 700
EcoRI GAATTC 5 cut(s) 872, 1909, 3185, 3869, 4321
EcoRII CCWGG 6 cut(s) 99, 967, 2425, 3106, 3637, 3702
EcoRV GATATC 1 cut(s) 4938
EcoT14I CCWWGG 8 cut(s) 20, 704, 1468, 3315, 3449, 3761, 4353, 4689
EcoT22I ATGCAT 5 cut(s) 982, 1511, 3061, 3298, 3692
EcoT38I GRGCYC 2 cut(s) 256, 1287
ErhI CCWWGG 8 cut(s) 20, 704, 1468, 3315, 3449, 3761, 4353, 4689
Esp3I CGTCTC 1 cut(s) 2503
FalI AAGNNNNNCTT 8 cut(s) 946, 978, 1077, 1109, 3812, 3844, 4489, 4521
FaqI GGGAC 5 cut(s) 2054, 2228, 3376, 3456, 3933
FauI CCCGC 1 cut(s) 2089
FauNDI CATATG 1 cut(s) 1942
FbaI TGATCA 5 cut(s) 405, 912, 3178, 4966, 4992
FblI GTMKAC 1 cut(s) 2186
Fnu4HI GCNGC 9 cut(s) 674, 1669, 2229, 2232, 2640, 3261, 3397, 4050, 4680
FriOI GRGCYC 2 cut(s) 256, 1287
Fsp4HI GCNGC 9 cut(s) 674, 1669, 2229, 2232, 2640, 3261, 3397, 4050, 4680
FspBI CTAG 4 cut(s) 1850, 2658, 5012, 5101
GlaI GCGC 3 cut(s) 1158, 3354, 4650
GluI GCNGC 9 cut(s) 674, 1669, 2229, 2232, 2640, 3261, 3397, 4050, 4680
GsuI CTGGAG 3 cut(s) 240, 4171, 4908
HaeII RGCGCY 2 cut(s) 3356, 4652
HaeIII GGCC 5 cut(s) 104, 2437, 2723, 3853, 3963
HapII CCGG 5 cut(s) 120, 1753, 2720, 3208, 3960
HgaI GACGC 2 cut(s) 25, 4578
HhaI GCGC 3 cut(s) 1159, 3355, 4651
Hin1I GRCGYC 3 cut(s) 17, 141, 4589
Hin6I GCGC 3 cut(s) 1157, 3353, 4649
HinP1I GCGC 3 cut(s) 1157, 3353, 4649
HincII GTYRAC 5 cut(s) 1999, 2336, 2506, 4140, 4222
HindII GTYRAC 5 cut(s) 1999, 2336, 2506, 4140, 4222
HindIII AAGCTT 4 cut(s) 1384, 1394, 2201, 3990
HpaII CCGG 5 cut(s) 120, 1753, 2720, 3208, 3960
Hpy99I CGWCG 3 cut(s) 10, 13, 3593
HpyCH4IV ACGT 2 cut(s) 141, 940
HpyF10VI GCNNNNNNNGC 9 cut(s) 251, 483, 1674, 1713, 1859, 2200, 4501, 4595, 4657
HpySE526I ACGT 2 cut(s) 141, 940
Hsp92I GRCGYC 3 cut(s) 17, 141, 4589
HspAI GCGC 3 cut(s) 1157, 3353, 4649
KroI GCCGGC 1 cut(s) 3959
KroNI GCCGGC 1 cut(s) 3961
Ksp22I TGATCA 5 cut(s) 405, 912, 3178, 4966, 4992
KspI CCGCGG 1 cut(s) 2195
LguI GCTCTTC 2 cut(s) 453, 3566
LmnI GCTCC 9 cut(s) 259, 1294, 2263, 2563, 3068, 3195, 3393, 4033, 4687
Lsp1109I GCAGC 8 cut(s) 1680, 2215, 2218, 2651, 3247, 3383, 4036, 4666
MabI ACCWGGT 1 cut(s) 967
MaeI CTAG 4 cut(s) 1850, 2658, 5012, 5101
MaeII ACGT 2 cut(s) 141, 940
MbiI CCGCTC 1 cut(s) 1924
MfeI CAATTG 2 cut(s) 726, 2574
MflI RGATCY 9 cut(s) 91, 1549, 1897, 2492, 2792, 4059, 4379, 4517, 4860
MhlI GDGCHC 4 cut(s) 256, 1107, 1287, 4964
MlyI GAGTC 7 cut(s) 305, 1247, 1765, 3766, 3809, 4209, 4478
MmeI TCCRAC 2 cut(s) 307, 590
Mph1103I ATGCAT 5 cut(s) 982, 1511, 3061, 3298, 3692
MroNI GCCGGC 1 cut(s) 3959
MroXI GAANNNNTTC 5 cut(s) 162, 942, 2309, 2624, 4722
MslI CAYNNNNRTG 5 cut(s) 477, 995, 1613, 3008, 4694
MspA1I CMGCKG 3 cut(s) 803, 2194, 4760
MspI CCGG 5 cut(s) 120, 1753, 2720, 3208, 3960
MspR9I CCNGG 7 cut(s) 101, 969, 1754, 2427, 3108, 3639, 3704
MunI CAATTG 2 cut(s) 726, 2574
Mva1269I GAATGC 8 cut(s) 175, 833, 2167, 2311, 3144, 3399, 3459, 3692
MvaI CCWGG 6 cut(s) 101, 969, 2427, 3108, 3639, 3704
MvnI CGCG 1 cut(s) 2194
MwoI GCNNNNNNNGC 9 cut(s) 251, 483, 1674, 1713, 1859, 2200, 4501, 4595, 4657
NaeI GCCGGC 1 cut(s) 3961
NciI CCSGG 1 cut(s) 1754
NcoI CCATGG 2 cut(s) 3761, 4689
NdeI CATATG 1 cut(s) 1942
NgoMIV GCCGGC 1 cut(s) 3959
NheI GCTAGC 1 cut(s) 1849
NlaIV GGNNCC 6 cut(s) 255, 702, 1176, 2494, 3070, 4035
NmeAIII GCCGAG 1 cut(s) 4650
NmuCI GTSAC 6 cut(s) 1604, 1768, 2537, 2606, 2964, 4590
NsiI ATGCAT 5 cut(s) 982, 1511, 3061, 3298, 3692
NspI RCATGY 5 cut(s) 844, 1821, 2228, 2607, 4280
NspV TTCGAA 1 cut(s) 4452
PaeI GCATGC 1 cut(s) 4280
PagI TCATGA 2 cut(s) 3181, 3741
PasI CCCWGGG 1 cut(s) 3638
PciI ACATGT 1 cut(s) 840
PciSI GCTCTTC 2 cut(s) 453, 3566
PcsI WCGNNNNNNNCGW 2 cut(s) 138, 2043
PctI GAATGC 8 cut(s) 175, 833, 2167, 2311, 3144, 3399, 3459, 3692
PdiI GCCGGC 1 cut(s) 3961
PdmI GAANNNNTTC 5 cut(s) 162, 942, 2309, 2624, 4722
PflFI GACNNNGTC 1 cut(s) 2045
PflMI CCANNNNNTGG 1 cut(s) 2267
PfoI TCCNGGA 1 cut(s) 3702
PkrI GCNGC 9 cut(s) 675, 1670, 2230, 2233, 2641, 3262, 3398, 4051, 4681
PleI GAGTC 7 cut(s) 305, 1246, 1765, 3766, 3808, 4208, 4477
PpsI GAGTC 7 cut(s) 305, 1246, 1765, 3766, 3808, 4208, 4477
PpuMI RGGWCCY 1 cut(s) 700
PscI ACATGT 1 cut(s) 840
PshBI ATTAAT 1 cut(s) 1334
PsiI TTATAA 3 cut(s) 2346, 2948, 3099
Psp5II RGGWCCY 1 cut(s) 700
Psp6I CCWGG 6 cut(s) 99, 967, 2425, 3106, 3637, 3702
PspGI CCWGG 6 cut(s) 99, 967, 2425, 3106, 3637, 3702
PspN4I GGNNCC 6 cut(s) 255, 702, 1176, 2494, 3070, 4035
PspPI GGNCC 3 cut(s) 333, 700, 3851
PspPPI RGGWCCY 1 cut(s) 700
PstNI CAGNNNCTG 1 cut(s) 4043
PsuI RGATCY 9 cut(s) 91, 1549, 1897, 2492, 2792, 4059, 4379, 4517, 4860
PsyI GACNNNGTC 1 cut(s) 2045
PvuII CAGCTG 2 cut(s) 803, 4760
RsaI GTAC 9 cut(s) 188, 495, 1519, 1556, 3212, 3557, 3880, 4365, 4575
RsaNI GTAC 9 cut(s) 187, 494, 1518, 1555, 3211, 3556, 3879, 4364, 4574
RseI CAYNNNNRTG 5 cut(s) 477, 995, 1613, 3008, 4694
SacII CCGCGG 1 cut(s) 2195
SapI GCTCTTC 2 cut(s) 453, 3566
SatI GCNGC 9 cut(s) 674, 1669, 2229, 2232, 2640, 3261, 3397, 4050, 4680
Sau96I GGNCC 3 cut(s) 333, 700, 3851
ScaI AGTACT 1 cut(s) 188
SchI GAGTC 7 cut(s) 305, 1247, 1765, 3766, 3809, 4209, 4478
ScrFI CCNGG 7 cut(s) 101, 969, 1754, 2427, 3108, 3639, 3704
SduI GDGCHC 4 cut(s) 256, 1107, 1287, 4964
SexAI ACCWGGT 1 cut(s) 967
SfcI CTRYAG 6 cut(s) 246, 1568, 2776, 2787, 3190, 4215
Sfr303I CCGCGG 1 cut(s) 2195
SfuI TTCGAA 1 cut(s) 4452
SgrBI CCGCGG 1 cut(s) 2195
SinI GGWCC 2 cut(s) 333, 700
SmiMI CAYNNNNRTG 5 cut(s) 477, 995, 1613, 3008, 4694
SmlI CTYRAG 8 cut(s) 212, 1094, 1984, 2207, 2900, 4015, 4231, 5090
SmoI CTYRAG 8 cut(s) 212, 1094, 1984, 2207, 2900, 4015, 4231, 5090
SphI GCATGC 1 cut(s) 4280
SsiI CCGC 8 cut(s) 673, 1028, 1110, 1924, 2096, 2192, 2194, 3841
SspI AATATT 1 cut(s) 1721
SspMI CTAG 4 cut(s) 1850, 2658, 5012, 5101
StyD4I CCNGG 7 cut(s) 99, 967, 1752, 2425, 3106, 3637, 3702
StyI CCWWGG 8 cut(s) 20, 704, 1468, 3315, 3449, 3761, 4353, 4689
TaiI ACGT 2 cut(s) 144, 943
TatI WGTACW 4 cut(s) 186, 1554, 3555, 4573
TauI GCSGC 1 cut(s) 676
TseFI GTSAC 6 cut(s) 1604, 1768, 2537, 2606, 2964, 4590
TseI GCWGC 8 cut(s) 1668, 2228, 2231, 2639, 3260, 3396, 4049, 4679
Tsp45I GTSAC 6 cut(s) 1604, 1768, 2537, 2606, 2964, 4590
TspGWI ACGGA 4 cut(s) 58, 101, 2485, 2758
Tth111I GACNNNGTC 1 cut(s) 2045
Van91I CCANNNNNTGG 1 cut(s) 2267
VpaK11BI GGWCC 2 cut(s) 333, 700
VspI ATTAAT 1 cut(s) 1334
XceI RCATGY 5 cut(s) 844, 1821, 2228, 2607, 4280
XcmI CCANNNNNNNNNTGG 1 cut(s) 4077
XmiI GTMKAC 1 cut(s) 2186
XmnI GAANNNNTTC 5 cut(s) 162, 942, 2309, 2624, 4722
XspI CTAG 4 cut(s) 1850, 2658, 5012, 5101
ZraI GACGTC 1 cut(s) 142
ZrmI AGTACT 1 cut(s) 188
Zsp2I ATGCAT 5 cut(s) 982, 1511, 3061, 3298, 3692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.