Rroxscaffold_7G00217350

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000526
Physical Location & Seq
Forward (+)
67808327 .. 67814900
6574 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00217350.1

Sequence Viewer

Length: 5121 bp
ATGGCGACGACGATGACGCCAAGGGAGCACATAGAAGATATACGGAAGATCAAGTTCTCCATCGGAGAAAAGCTCAACCCTTTAACGGAGGATCTTCACCAGGCCGTCAAGAACCTCTCCGGCGAGCTTTACGCTAAAGACGTCCACTTTTTCATGGAACTCGTTCAGAATGCAGAAGACAACGAGTACTCAGAGGGGGTTGATCCATCCCTTGAGTTTTTGATTACGTCCCGGGATATAACGGCTACAGGTGCTCCAGCCACATTGCTGGTTTTCAATAATGAGAAGGGGTTTTCTTCCAAAAACATAGACTCCATTTGCAGTGTTGGACGGTCCACCAAGAAAGGCAACAGGAAGCGTGGTTATATTGGGGAGAAAGGAATTGGCTTCAAGAGTGTGTTTCTGATCACTGCTCAGCCTTACATTTTTAGTAATGGTTATCAGATAAGGTTTAGCGAAGAGCCTTGTTCACATTGCAATGTTGGCTACATAGTACCTGAATGGGTTGAGCAGAATCCCACACTGTCTGATATAGAAAAGATATACGGTTCTGCTACTGCCCTTCCTACCACAACACTGATCTTACCTCTGAAGCCCGACAAGGTTGAACCTGTGAAGAAACAACTCTCAATCATTCATCCAGAAGTTCTCTTGTTCCTATCAAAGATTAAGCGGCTCTCTGTTAGGCAAGACAAAGAGGATCCAAGCCAGAACACTGTGAGTGCAATTGAGATAGCAAGCCAGACTGATTTTGTGGAAAGGAAGAACATCGATGCTCAATCCTACACTCAGCGTCTTTCAGCTGAAGAAAATGATGGAAATGGAGAATGCAATTATTACATGTGGAAACAAAAGTTTCCTGTCAGGCAGGAATTCAGAGTTGAGAGGAGAATGGAGGTGGATGAGTGGGTGATCACTCTTGCATTTCCAAATGGAGAACGTCTTCGAAGAGCAAGGAACTCTTCGCCTGGTGTTTATGCATTTCTTCCCACTGAGATGGTCACCAACTTTCCATTCATAATACAGGCGGATTTTCTTTTAGCATCTTCAAGAGAGACAATCCTACTTGACAACAAGTGGAATCAAGGGATTCTTGAGTGTGTGCCCACTGCATTTGCCCATGCCTTTAACTCTTTTGTGAAAACTGTGGTAGGTGCGCCAGCATCAAGTTTGGCTCGTATGTTCAGATTCTTGCCTGTTCAGGGCTCTTCCTATAAAGAATTAAATGCTGTAAGGGAGTCGATAAAAGCGAAGATGGTTGAGGAAGACATTATACCAGTTGAGCCTGAAATGGAGCAGAGGTTCTTCCACAAGCCTGGTGAAGTGGGAAGATTAATGCCTGCCTTTTGGTCTATTTTAAGGAAGGCGAGGGTTCAAGGTGTAAGCTGGATTAAGCTTTCATCACATGGAAGATTCGTTCTGTCTTATTCCTTTGACAGCGAGGAATATGATCCTATACTAAGTTTCCTTGGAGTTGAACCAGTGAATGATGATTGGTATGTTAAATGCATACAGGGTACTTCTAATCTTGTTGATGGAGTGTCAGAAGATGTGTACTTAGAGCTTCTACTCTTTATCGCTAACAATTGGGGTTCTAAATTTGGTCACACCAACATGAAGAACATACCATTGATAAAATATGCAGACTATGGAAATGAATCTTTGTGCAGCATAAGCGAAATCCAGAAAGGTGAATGTAGAGTTTGCTTATCGAGCAAATATTCTCATGTTTCATGGCTGATTGAATGCAACCGGGAATTTAATTCTGTGACTCGTCTTCTCTTTATGCCAAAAGCCACTCAGGAAGCCATCTGGTGGGCATGCTCTGGGAAGGATACATTGAAGAAATGGCTAGCTGATCAAGCTAAGGTTGATTCTGTCGACGTCTATAAATATGCAGATGATCTCCTTCAGAATTCTCTTAATGACCTGAAGCTTGTTATTGCATATGTTCATTTCTTATATCACTCGGCTGATAATGATTTTATCTCAAGTCGGGAAGTAGACCGTTTATGTGGAATTATGCCACTAGTGGATGGCAATGGGAAAATCAGCAGAAGTAGAAAAGGGGTTGTTATTGTTCCTGCCAATGGAAGCAAATGGTCTGGATTGACTGATTCTTATTTGTGGAGAAAAGAAGGCCATGTTGAATTAGGAGAGCGTTATATGTATTCTGGCTATTTTGCTGGTAAGCATACACCGGGGAAGAAGCTTCTTGAGTTCCTTATAAAACATGCTGCTGCTTTGGATGTCCCTCATATTTCTGCTCCAAGTGATGGTATCTCGTCTGTATCTGCACCACTAACTAAGCAGAATGCCTTCTTGCTTTTGGATTGGATTCGTCAACTGAATTATAAAAGGGTTGGCATCCCTCAGAAGTTCTTGGCTTGCATAAAGGAAGGTAGTTGGCTGAAAGTTACTTTGAATGGCTCTCCTGGTTATAGGCCACCATCCCAGTCATTTCTGCTTAACTCATCATGGGGAAATACTTTGCAGAATGGATCCGTCTCTGTTGACATTCCATTGATTGATACGAGCTTTTATGGTGAGAGCATTAGTGGATACAAGGAGGAGCTAAAGACAATTGGGGTCATGTTTGAATATAGCGAAGCATGTGACTTCATTGGGAAGCATTTCATGTCTCTGGCAGCTTCATCCTCTTTAACTAGAGGAAATGTGCATTCCATTCTGCGTTTCATCAGGTTTTTAAGGGACAAACTTCTTCCTCCGGCCGATTTTATCTCTGCTATTAAAAAAGGGAACTGGCTGAAGACTTCCGTTGGCTACAGGTCTCCTGTAGGATCTGTTTTGTCAGACAAGGAGTGGAATGTTGCCTCCAAAATTAGCAACATTCCCTTCATTGATCAGGACTTTTACGGTGATGAAATCTGTAATTTCAGAACTGAACTTGAGTTGCTCGGTGTTGTAATCTCCTTCAACAGAAGTTACCAACTTATAATTGACAACCTGAAGTCACCATCTTCCATAACTTCTTGGACACCTCAGGTTGTCATCTTAATGCTCAACTGTATGGCTCTATCCAAGTCATCTGATAAACTTGTCAATGCATTGAAAGGAGCCAAGTGCCTGAAGACAAGTGTTGGTTACAAGTGTCCAGGGGACTGTCTCTTGTTTCATCACGAATGGGGTTGCATTCTTCAGGTCGTCAATGGTCTTCCCGTGATTGATCATGAATTCTATGGGAGCAACATTTTCCTGTACATAAATGAACTGAAAAAAATTGGTGTGGTGGTTGATTTTGAAGAGGCATCAAAAGTATTTGCTCGATATTTCAGGCACTATGCATCATCAGCTTCCATCACCAAGGAAAATGTTGCATCATTGCTTCTGTGTTACAGAGCGCTGAAGGGGACTCCATATAAATTTCCTGCTGATCTTAGGAGCTGCATCGGTGAGGTCAAGTGGTTGCGTAGTCGTTTTGGTGATTTCAGATGTCCAAGGGAGTGCATTCTCTTTAGTCCCGAGTGGGAATCTATATCCCCAATAACTCTACTTCCTTTCATTGATGATTCTGACAGTTACTATGGAAAGAGCATACATGAGTACAGAAAAGAGCTGAAGAGCTTGGGGGTTGTCGTCGAATACAAGGAAGGAGTTAAGTTTGTGGCTTATGGTCTTTATCTCCCTAGGGATGCCGGCCGTATTTTTCCTGTAAATGCACTTGCGTTGCTGGAATGCATACGAGTTTTCCAGGAGAAAGGTCACGCCTTTCTTGATAATTCTGATTTCATGAAGAAAATTTCTCAACCATGGATAAAGACTCATGCTGGTTATAGGTCTCCCAAAGAGTCTTTGTTGTTTGATTCCATGTTTGGCTTATATTTGAAGCGGACTGATGGGCCTTTCATTGATGAAGAATTCTATGGTACTAAGATTGCATCTTACAAAAAAGAGCTGTCTGCATTGGGGGTTATTGTTGAAGCTGAGAAAGGATGTCCCCTGATTGCCAGCCACCTTGATGTTCATTATGAGATGGAAGCATTTGTTCGGCTGTATAATTACTTGAGTGCATTCAAGTGGGAACCAGACACTGATGCTGACAGATGGATCTGGGTTCCAAAAGGAGAGTGGGTTCGTCCTGATGATTGTGTTATATATGACAAGGATGGGCTCTTTGGTTTACAATTGACTGTTTTGGAGAAACATTATGAGCAGAAACTTCTATTTTTCTTCTCCAGTGCGTTTAGAGTTAAATCTCATCCTTCAGTTGATGATTACTTGAAGGTTTGGAAGGTTTGGGAAAGTTCTGGAAGTGCATTGTCGCATGCTGATTGTTGCAAGTTTTGGAGTTATATCTCGAAGCACTGGAATTCTAAGACAGAAAAGAGTCAAAAGACTGTTGCTGATGCCTTGGAGAAAGTACCTGTCAGTTCTGGATCTGAAGGAATCTTGCTGTGCAACAAGTCTGATGTTTTCATTGCTGATGACCTTCAACTGAAGTATCTATTCGAACAGTCTTCTCCTGAGTCTATTTTTGTATGGTACCCTCAGCCAAGTTTGCCTTCCATTCCAAGATCCAAGTTGCTTGACATTTATCGAAAAATTGGTGTTCGCACTATATCTGAATCTGTACAGAAGGAAGAGGCGTCACTTGCAGATGATGTTGAAGTAGTCTCTTTGCCGAGGGAAAAGCTGATTAAGAAAGCGCTGTTCAGGCTGATTCTTGGTTTTCTTGCTGCTCCTCCCATGGAAATGGAAGCTGAGATGAGGCTTGAAGCTGTTCAATTCCTTGTGAATGTTACTGTGGTGGTAACAGCTGAACCAATCTCTGTAAGCTATAATCTACCTTTATCTTCTGGTAAAGTTTTGAATGTGAGAGGAAGCCGAAAAATACGGTTTGACAGAGAAAATTCAACGATCTTCACTCAGAAAATGGACAAGTCTGGAGGGCAGAAGAGTATCATTGAGTTTGCTACTTTTTTCTCCGAAGCGATATCTGAGAGTGTGTTATGGGAGAGCACTGATCATATAGATTCACTCTCTGAGCTGATCAAGGTGGCTTATTTGCTAGACTTCAATAAGGAAGCAGTTGATTTCTTGATGAAGTCCAAGAATTTGCAAACTTTTATGGAGGATGAGGAGTTTCTCAAGACTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1706

Amino Acids

193.5

Weight (kDa)

6.47

Isoelectric Point (pI)

39.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 30 - 146 1.9e-11 Sacsin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28020 AT3G48770 AT3G48770
fragaria_vesca FvH4_5g09020 FvH4_5g09020
malus_domestica MD06G1092400.v1.1 MD06G1134600.v1.1 MD06G1134700.v1.1 MD06G1134800.v1.1 MD06G1135200.v1.1 MD06G1135400.v1.1 MD06G1135500.v1.1 MD08G1137600.v1.1 MD15G1395100.v1.1
prunus_persica Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145600_v2.0.a1 Prupe.5G145800_v2.0.a1 Prupe.5G145900_v2.0.a1
pyrus_communis pycom06g12550 pycom06g12570 pycom06g12580 pycom06g12590 pycom06g12610 pycom06g12630 pycom06g12640 pycom06g12650 pycom08g06280
rosa_chinensis RchiOBHm_Chr6g0249321 RchiOBHm_Chr6g0275111 RchiOBHm_Chr7g0189191 RchiOBHm_Chr7g0189261
rosa_laevigata RLG00000004622 RLG00000004623 RLG00000008237 RLG00000008238 RLG00000023303 RLG00000035187
rosa_multiflora Rmu_co8294311.1_g000001 Rmu_co8389833.1_g000001 Rmu_sc0001044.1_g000004 Rmu_sc0001329.1_g000011 Rmu_sc0006012.1_g000010 Rmu_sc0007286.1_g000032 Rmu_sc0009360.1_g000016 Rmu_sc0011817.1_g000001 Rmu_sc0015050.1_g000001
rosa_roxburghii Rroxscaffold_1G00064000 Rroxscaffold_3G00265460 Rroxscaffold_3G00265580 Rroxscaffold_3G00265640 Rroxscaffold_3G00265690 Rroxscaffold_3G00265700 Rroxscaffold_7G00193620 Rroxscaffold_7G00204550 Rroxscaffold_7G00217350
rosa_rugosa Rorug01G0465500 Rorug06G0088500 Rorug06G0496700 Rorug06G0496700
rosa_samantha Rh1AG064800 Rh2BG015100 Rh2BG493500 Rh2DG017300 Rh2DG502500 Rh4BG208500 Rh5BG528500 Rh5CG552900 Rh6CG207800 Rh6DG197900 Rh6DG198000 Rh7AG101300 Rh7AG101400 Rh7AG101600 Rh7BG104100 Rh7BG104200 Rh7CG105600 Rh7CG105700 Rh7DG103200 Rh7DG103400
rosa_wichuraiana Rw1G007300 Rw5G034670 Rw7G008750 Rw7G008780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2228, 2355, 2957
AatII GACGTC 2 cut(s) 144, 1887
Acc65I GGTACC 1 cut(s) 4503
AccB1I GGYRCC 1 cut(s) 4503
AccB7I CCANNNNNTGG 2 cut(s) 1815, 2276
AccI GTMKAC 2 cut(s) 1881, 2004
AciI CCGC 3 cut(s) 673, 1028, 3850
AcoI YGGCCR 2 cut(s) 2730, 3658
AcyI GRCGYC 4 cut(s) 17, 141, 1884, 4607
AfeI AGCGCT 2 cut(s) 3363, 4668
AfiI CCNNNNNNNGG 5 cut(s) 85, 1202, 1815, 2090, 2276
AflIII ACRYGT 1 cut(s) 840
AhlI ACTAGT 1 cut(s) 2029
AjnI CCWGG 6 cut(s) 99, 967, 1315, 2434, 3115, 3711
AjuI GAANNNNNNNTTGG 4 cut(s) 998, 1030, 4507, 4539
AloI GAACNNNNNNTCC 6 cut(s) 1286, 1318, 1401, 1433, 4077, 4109
Alw21I GWGCWC 3 cut(s) 30, 256, 4982
Alw26I GTCTC 7 cut(s) 1049, 2512, 2646, 2796, 3131, 3804, 4639
AlwNI CAGNNNCTG 1 cut(s) 4052
Ama87I CYCGRG 2 cut(s) 231, 3482
Aor51HI AGCGCT 2 cut(s) 3363, 4668
AoxI GGCC 6 cut(s) 102, 2140, 2444, 2730, 3658, 3860
ApeKI GCWGC 6 cut(s) 1668, 2237, 2240, 2648, 3405, 4697
AseI ATTAAT 1 cut(s) 1334
Asp700I GAANNNNTTC 5 cut(s) 162, 942, 2318, 2633, 4740
Asp718I GGTACC 1 cut(s) 4503
AspA2I CCTAGG 1 cut(s) 3647
AspLEI GCGC 3 cut(s) 1159, 3364, 4669
AspS9I GGNCC 2 cut(s) 333, 3860
AsuC2I CCSGG 4 cut(s) 232, 233, 1754, 2202
AsuII TTCGAA 2 cut(s) 946, 4470
AsuNHI GCTAGC 1 cut(s) 1852
AvaI CYCGRG 2 cut(s) 231, 3482
AvaII GGWCC 1 cut(s) 333
AvrII CCTAGG 1 cut(s) 3647
AxyI CCTNAGG 1 cut(s) 3003
BaeGI GKGCMC 1 cut(s) 1107
BaeI ACNNNNGTAYC 2 cut(s) 4445, 4478
BamHI GGATCC 2 cut(s) 700, 2501
BanI GGYRCC 1 cut(s) 4503
BanII GRGCYC 2 cut(s) 1208, 4134
BarI GAAGNNNNNNTAC 2 cut(s) 1257, 1289
BbsI GAAGAC 8 cut(s) 183, 935, 1272, 1769, 2777, 3098, 3167, 4470
Bbv12I GWGCWC 3 cut(s) 30, 256, 4982
BbvCI CCTCAGC 1 cut(s) 4509
BbvI GCAGC 6 cut(s) 1680, 2224, 2227, 2660, 3392, 4684
BceAI ACGGC 3 cut(s) 89, 258, 3645
BciT130I CCWGG 6 cut(s) 101, 969, 1317, 2436, 3117, 3713
BciVI GTATCC 2 cut(s) 1828, 2555
BclI TGATCA 7 cut(s) 405, 912, 1858, 2863, 3187, 4984, 5010
BcnI CCSGG 4 cut(s) 232, 233, 1754, 2202
BcoDI GTCTC 7 cut(s) 1049, 2512, 2646, 2796, 3131, 3804, 4639
BcuI ACTAGT 1 cut(s) 2029
BfaI CTAG 6 cut(s) 1853, 2030, 2667, 3648, 5030, 5119
BfmI CTRYAG 3 cut(s) 246, 2785, 2796
BfoI RGCGCY 2 cut(s) 3365, 4670
BfuI GTATCC 2 cut(s) 1828, 2555
BisI GCNGC 7 cut(s) 674, 1669, 2238, 2241, 2649, 3406, 4698
BlnI CCTAGG 1 cut(s) 3647
BlpI GCTNAGC 1 cut(s) 414
BlsI GCNGC 7 cut(s) 675, 1670, 2239, 2242, 2650, 3407, 4699
BmcAI AGTACT 1 cut(s) 188
Bme18I GGWCC 1 cut(s) 333
BmeT110I CYCGRG 2 cut(s) 231, 3482
BmgT120I GGNCC 2 cut(s) 333, 3860
BmiI GGNNCC 6 cut(s) 702, 2503, 3079, 4044, 4077, 4505
BmrI ACTGGG 1 cut(s) 2449
BmtI GCTAGC 1 cut(s) 1856
BmuI ACTGGG 1 cut(s) 2449
BpiI GAAGAC 8 cut(s) 183, 935, 1272, 1769, 2777, 3098, 3167, 4470
BplI GAGNNNNNCTC 2 cut(s) 57, 89
BpmI CTGGAG 3 cut(s) 240, 4180, 4926
Bpu10I CCTNAGC 2 cut(s) 1866, 4509
Bpu1102I GCTNAGC 1 cut(s) 414
Bpu14I TTCGAA 2 cut(s) 946, 4470
BpuEI CTTGAG 7 cut(s) 233, 1115, 1975, 2237, 2930, 4045, 5093
BpuMI CCSGG 4 cut(s) 232, 233, 1754, 2202
Bsa29I ATCGAT 1 cut(s) 771
BsaHI GRCGYC 4 cut(s) 17, 141, 1884, 4607
BsaI GGTCTC 2 cut(s) 2796, 3804
BsaXI ACNNNNNCTCC 8 cut(s) 238, 268, 296, 326, 1286, 1316, 4077, 4107
Bsc4I CCNNNNNNNGG 5 cut(s) 85, 1202, 1815, 2090, 2276
Bse118I RCCGGY 1 cut(s) 3656
Bse1I ACTGG 6 cut(s) 1277, 1481, 2455, 2768, 4197, 4331
Bse21I CCTNAGG 1 cut(s) 3003
Bse3DI GCAATG 6 cut(s) 263, 472, 484, 2047, 3341, 4437
BseBI CCWGG 6 cut(s) 101, 969, 1317, 2436, 3117, 3713
BseCI ATCGAT 1 cut(s) 771
BseLI CCNNNNNNNGG 5 cut(s) 85, 1202, 1815, 2090, 2276
BseMI GCAATG 6 cut(s) 263, 472, 484, 2047, 3341, 4437
BseNI ACTGG 6 cut(s) 1277, 1481, 2455, 2768, 4197, 4331
BseRI GAGGAG 4 cut(s) 901, 2585, 4692, 5114
BseSI GKGCMC 1 cut(s) 1107
BseX3I CGGCCG 2 cut(s) 2730, 3658
BseXI GCAGC 6 cut(s) 1680, 2224, 2227, 2660, 3392, 4684
BsgI GTGCAG 2 cut(s) 1687, 2280
Bsh1285I CGRYCG 2 cut(s) 2733, 3661
BshFI GGCC 6 cut(s) 104, 2142, 2446, 2732, 3660, 3862
BshNI GGYRCC 1 cut(s) 4503
BshVI ATCGAT 1 cut(s) 771
BsiEI CGRYCG 2 cut(s) 2733, 3661
BsiHKAI GWGCWC 3 cut(s) 30, 256, 4982
BsiHKCI CYCGRG 2 cut(s) 231, 3482
BsiSI CCGG 6 cut(s) 120, 232, 1753, 2201, 2729, 3657
BslFI GGGAC 7 cut(s) 214, 2237, 2726, 3134, 3385, 3465, 3942
BslI CCNNNNNNNGG 5 cut(s) 85, 1202, 1815, 2090, 2276
BsmAI GTCTC 7 cut(s) 1049, 2512, 2646, 2796, 3131, 3804, 4639
BsmBI CGTCTC 1 cut(s) 2512
BsmFI GGGAC 7 cut(s) 214, 2237, 2726, 3134, 3385, 3465, 3942
BsmI GAATGC 9 cut(s) 175, 833, 1751, 2320, 2680, 3153, 3468, 3701, 4031
BsnI GGCC 6 cut(s) 104, 2142, 2446, 2732, 3660, 3862
Bso31I GGTCTC 2 cut(s) 2796, 3804
BsoBI CYCGRG 2 cut(s) 231, 3482
Bsp119I TTCGAA 2 cut(s) 946, 4470
Bsp1286I GDGCHC 6 cut(s) 30, 256, 1107, 1208, 4134, 4982
Bsp1407I TGTACA 2 cut(s) 3219, 4591
Bsp1720I GCTNAGC 1 cut(s) 414
Bsp19I CCATGG 2 cut(s) 3770, 4707
BspACI CCGC 3 cut(s) 673, 1028, 3850
BspANI GGCC 6 cut(s) 104, 2142, 2446, 2732, 3660, 3862
BspDI ATCGAT 1 cut(s) 771
BspHI TCATGA 2 cut(s) 3190, 3750
BspLI GGNNCC 6 cut(s) 702, 2503, 3079, 4044, 4077, 4505
BspOI GCTAGC 1 cut(s) 1856
BspQI GCTCTTC 4 cut(s) 453, 943, 1213, 3575
BspT104I TTCGAA 2 cut(s) 946, 4470
BspT107I GGYRCC 1 cut(s) 4503
BspTNI GGTCTC 2 cut(s) 2796, 3804
BsrDI GCAATG 6 cut(s) 263, 472, 484, 2047, 3341, 4437
BsrFI RCCGGY 1 cut(s) 3656
BsrGI TGTACA 2 cut(s) 3219, 4591
BsrI ACTGG 6 cut(s) 1277, 1481, 2455, 2768, 4197, 4331
BssAI RCCGGY 1 cut(s) 3656
BssNI GRCGYC 4 cut(s) 17, 141, 1884, 4607
BssT1I CCWWGG 8 cut(s) 20, 1468, 3324, 3458, 3647, 3770, 4371, 4707
Bst2UI CCWGG 6 cut(s) 101, 969, 1317, 2436, 3117, 3713
Bst6I CTCTTC 8 cut(s) 453, 943, 967, 1213, 3258, 3575, 4596, 4910
BstACI GRCGYC 4 cut(s) 17, 141, 1884, 4607
BstAUI TGTACA 2 cut(s) 3219, 4591
BstBI TTCGAA 2 cut(s) 946, 4470
BstDSI CCRYGG 2 cut(s) 3770, 4707
BstEII GGTNACC 1 cut(s) 1000
BstH2I RGCGCY 2 cut(s) 3365, 4670
BstHHI GCGC 3 cut(s) 1159, 3364, 4669
BstMAI GTCTC 7 cut(s) 1049, 2512, 2646, 2796, 3131, 3804, 4639
BstMCI CGRYCG 2 cut(s) 2733, 3661
BstNI CCWGG 6 cut(s) 101, 969, 1317, 2436, 3117, 3713
BstNSI RCATGY 5 cut(s) 844, 1824, 2237, 2616, 4289
BstPI GGTNACC 1 cut(s) 1000
BstSFI CTRYAG 3 cut(s) 246, 2785, 2796
BstSLI GKGCMC 1 cut(s) 1107
BstV1I GCAGC 6 cut(s) 1680, 2224, 2227, 2660, 3392, 4684
BstV2I GAAGAC 8 cut(s) 183, 935, 1272, 1769, 2777, 3098, 3167, 4470
BstX2I RGATCY 7 cut(s) 91, 700, 2501, 2801, 4068, 4397, 4535
BstXI CCANNNNNNTGG 4 cut(s) 268, 997, 1316, 4714
BstYI RGATCY 7 cut(s) 91, 700, 2501, 2801, 4068, 4397, 4535
BstZI CGGCCG 2 cut(s) 2730, 3658
Bsu15I ATCGAT 1 cut(s) 771
Bsu36I CCTNAGG 1 cut(s) 3003
BsuI GTATCC 2 cut(s) 1828, 2555
BsuRI GGCC 6 cut(s) 104, 2142, 2446, 2732, 3660, 3862
BsuTUI ATCGAT 1 cut(s) 771
BtgI CCRYGG 2 cut(s) 3770, 4707
BtsI GCAGTG 3 cut(s) 328, 408, 1107
CaiI CAGNNNCTG 1 cut(s) 4052
CciI TCATGA 2 cut(s) 3190, 3750
CfoI GCGC 3 cut(s) 1159, 3364, 4669
Cfr10I RCCGGY 1 cut(s) 3656
Cfr13I GGNCC 2 cut(s) 333, 3860
Cfr9I CCCGGG 1 cut(s) 231
ClaI ATCGAT 1 cut(s) 771
CseI GACGC 3 cut(s) 25, 782, 4596
CspCI CAANNNNNGTGG 2 cut(s) 1096, 1131
EaeI YGGCCR 2 cut(s) 2730, 3658
EagI CGGCCG 2 cut(s) 2730, 3658
Eam1104I CTCTTC 8 cut(s) 453, 943, 967, 1213, 3258, 3575, 4596, 4910
EarI CTCTTC 8 cut(s) 453, 943, 967, 1213, 3258, 3575, 4596, 4910
EciI GGCGGA 1 cut(s) 1043
EclXI CGGCCG 2 cut(s) 2730, 3658
Eco130I CCWWGG 8 cut(s) 20, 1468, 3324, 3458, 3647, 3770, 4371, 4707
Eco24I GRGCYC 2 cut(s) 1208, 4134
Eco31I GGTCTC 2 cut(s) 2796, 3804
Eco32I GATATC 1 cut(s) 4956
Eco47I GGWCC 1 cut(s) 333
Eco47III AGCGCT 2 cut(s) 3363, 4668
Eco52I CGGCCG 2 cut(s) 2730, 3658
Eco81I CCTNAGG 1 cut(s) 3003
Eco88I CYCGRG 2 cut(s) 231, 3482
Eco91I GGTNACC 1 cut(s) 1000
EcoO65I GGTNACC 1 cut(s) 1000
EcoRI GAATTC 5 cut(s) 872, 1915, 3194, 3878, 4330
EcoRII CCWGG 6 cut(s) 99, 967, 1315, 2434, 3115, 3711
EcoRV GATATC 1 cut(s) 4956
EcoT14I CCWWGG 8 cut(s) 20, 1468, 3324, 3458, 3647, 3770, 4371, 4707
EcoT22I ATGCAT 5 cut(s) 982, 1511, 3070, 3307, 3701
EcoT38I GRGCYC 2 cut(s) 1208, 4134
ErhI CCWWGG 8 cut(s) 20, 1468, 3324, 3458, 3647, 3770, 4371, 4707
Esp3I CGTCTC 1 cut(s) 2512
FalI AAGNNNNNCTT 6 cut(s) 946, 978, 1077, 1109, 4507, 4539
FaqI GGGAC 7 cut(s) 214, 2237, 2726, 3134, 3385, 3465, 3942
FauNDI CATATG 1 cut(s) 1948
FbaI TGATCA 7 cut(s) 405, 912, 1858, 2863, 3187, 4984, 5010
FblI GTMKAC 2 cut(s) 1881, 2004
Fnu4HI GCNGC 7 cut(s) 674, 1669, 2238, 2241, 2649, 3406, 4698
FriOI GRGCYC 2 cut(s) 1208, 4134
Fsp4HI GCNGC 7 cut(s) 674, 1669, 2238, 2241, 2649, 3406, 4698
FspBI CTAG 6 cut(s) 1853, 2030, 2667, 3648, 5030, 5119
GlaI GCGC 3 cut(s) 1158, 3363, 4668
GluI GCNGC 7 cut(s) 674, 1669, 2238, 2241, 2649, 3406, 4698
GsuI CTGGAG 3 cut(s) 240, 4180, 4926
HaeII RGCGCY 2 cut(s) 3365, 4670
HaeIII GGCC 6 cut(s) 104, 2142, 2446, 2732, 3660, 3862
HapII CCGG 6 cut(s) 120, 232, 1753, 2201, 2729, 3657
HgaI GACGC 3 cut(s) 25, 782, 4596
HhaI GCGC 3 cut(s) 1159, 3364, 4669
Hin1I GRCGYC 4 cut(s) 17, 141, 1884, 4607
Hin6I GCGC 3 cut(s) 1157, 3362, 4667
HinP1I GCGC 3 cut(s) 1157, 3362, 4667
HincII GTYRAC 3 cut(s) 1882, 2345, 2515
HindII GTYRAC 3 cut(s) 1882, 2345, 2515
HindIII AAGCTT 3 cut(s) 1394, 1934, 2210
HpaII CCGG 6 cut(s) 120, 232, 1753, 2201, 2729, 3657
Hpy166II GTNNAC 9 cut(s) 145, 336, 470, 1555, 1882, 2005, 2345, 2515, 4142
Hpy8I GTNNAC 9 cut(s) 145, 336, 470, 1555, 1882, 2005, 2345, 2515, 4142
Hpy99I CGWCG 4 cut(s) 10, 13, 1886, 3602
HpyCH4IV ACGT 4 cut(s) 141, 227, 940, 1884
HpySE526I ACGT 4 cut(s) 141, 227, 940, 1884
Hsp92I GRCGYC 4 cut(s) 17, 141, 1884, 4607
HspAI GCGC 3 cut(s) 1157, 3362, 4667
KpnI GGTACC 1 cut(s) 4507
KroI GCCGGC 1 cut(s) 3656
KroNI GCCGGC 1 cut(s) 3658
Ksp22I TGATCA 7 cut(s) 405, 912, 1858, 2863, 3187, 4984, 5010
LguI GCTCTTC 4 cut(s) 453, 943, 1213, 3575
LmnI GCTCC 9 cut(s) 25, 259, 1294, 2272, 2572, 3077, 3204, 3402, 4705
Lsp1109I GCAGC 6 cut(s) 1680, 2224, 2227, 2660, 3392, 4684
MaeI CTAG 6 cut(s) 1853, 2030, 2667, 3648, 5030, 5119
MaeII ACGT 4 cut(s) 141, 227, 940, 1884
MfeI CAATTG 4 cut(s) 726, 1585, 2583, 4145
MflI RGATCY 7 cut(s) 91, 700, 2501, 2801, 4068, 4397, 4535
MhlI GDGCHC 6 cut(s) 30, 256, 1107, 1208, 4134, 4982
MlyI GAGTC 8 cut(s) 305, 1247, 1765, 3367, 3775, 3818, 4357, 4496
MmeI TCCRAC 1 cut(s) 307
Mph1103I ATGCAT 5 cut(s) 982, 1511, 3070, 3307, 3701
MroNI GCCGGC 1 cut(s) 3656
MroXI GAANNNNTTC 5 cut(s) 162, 942, 2318, 2633, 4740
MslI CAYNNNNRTG 7 cut(s) 477, 995, 1613, 3017, 3978, 4036, 4712
MspA1I CMGCKG 2 cut(s) 803, 4778
MspI CCGG 6 cut(s) 120, 232, 1753, 2201, 2729, 3657
MunI CAATTG 4 cut(s) 726, 1585, 2583, 4145
Mva1269I GAATGC 9 cut(s) 175, 833, 1751, 2320, 2680, 3153, 3468, 3701, 4031
MvaI CCWGG 6 cut(s) 101, 969, 1317, 2436, 3117, 3713
NaeI GCCGGC 1 cut(s) 3658
NciI CCSGG 4 cut(s) 232, 233, 1754, 2202
NcoI CCATGG 2 cut(s) 3770, 4707
NdeI CATATG 1 cut(s) 1948
NgoMIV GCCGGC 1 cut(s) 3656
NheI GCTAGC 1 cut(s) 1852
NlaIV GGNNCC 6 cut(s) 702, 2503, 3079, 4044, 4077, 4505
NmeAIII GCCGAG 2 cut(s) 1949, 4668
NmuCI GTSAC 7 cut(s) 1000, 1604, 1768, 2615, 2973, 3722, 4608
NsiI ATGCAT 5 cut(s) 982, 1511, 3070, 3307, 3701
NspI RCATGY 5 cut(s) 844, 1824, 2237, 2616, 4289
NspV TTCGAA 2 cut(s) 946, 4470
PaeI GCATGC 2 cut(s) 1824, 4289
PagI TCATGA 2 cut(s) 3190, 3750
PciI ACATGT 1 cut(s) 840
PciSI GCTCTTC 4 cut(s) 453, 943, 1213, 3575
PcsI WCGNNNNNNNCGW 2 cut(s) 138, 1247
PctI GAATGC 9 cut(s) 175, 833, 1751, 2320, 2680, 3153, 3468, 3701, 4031
PdiI GCCGGC 1 cut(s) 3658
PdmI GAANNNNTTC 5 cut(s) 162, 942, 2318, 2633, 4740
PflMI CCANNNNNTGG 2 cut(s) 1815, 2276
PfoI TCCNGGA 1 cut(s) 3711
PkrI GCNGC 7 cut(s) 675, 1670, 2239, 2242, 2650, 3407, 4699
PleI GAGTC 8 cut(s) 305, 1246, 1765, 3367, 3775, 3817, 4356, 4495
PpsI GAGTC 8 cut(s) 305, 1246, 1765, 3367, 3775, 3817, 4356, 4495
PscI ACATGT 1 cut(s) 840
PshBI ATTAAT 1 cut(s) 1334
PsiI TTATAA 3 cut(s) 2228, 2355, 2957
Psp6I CCWGG 6 cut(s) 99, 967, 1315, 2434, 3115, 3711
PspEI GGTNACC 1 cut(s) 1000
PspGI CCWGG 6 cut(s) 99, 967, 1315, 2434, 3115, 3711
PspN4I GGNNCC 6 cut(s) 702, 2503, 3079, 4044, 4077, 4505
PspPI GGNCC 2 cut(s) 333, 3860
PstNI CAGNNNCTG 1 cut(s) 4052
PsuI RGATCY 7 cut(s) 91, 700, 2501, 2801, 4068, 4397, 4535
PvuII CAGCTG 2 cut(s) 803, 4778
RseI CAYNNNNRTG 7 cut(s) 477, 995, 1613, 3017, 3978, 4036, 4712
SalI GTCGAC 1 cut(s) 1880
SapI GCTCTTC 4 cut(s) 453, 943, 1213, 3575
SatI GCNGC 7 cut(s) 674, 1669, 2238, 2241, 2649, 3406, 4698
Sau96I GGNCC 2 cut(s) 333, 3860
ScaI AGTACT 1 cut(s) 188
SchI GAGTC 8 cut(s) 305, 1247, 1765, 3367, 3775, 3818, 4357, 4496
SduI GDGCHC 6 cut(s) 30, 256, 1107, 1208, 4134, 4982
SfcI CTRYAG 3 cut(s) 246, 2785, 2796
SfuI TTCGAA 2 cut(s) 946, 4470
SinI GGWCC 1 cut(s) 333
SmaI CCCGGG 1 cut(s) 233
SmiMI CAYNNNNRTG 7 cut(s) 477, 995, 1613, 3017, 3978, 4036, 4712
SmlI CTYRAG 7 cut(s) 212, 1094, 1990, 2216, 2909, 4024, 5108
SmoI CTYRAG 7 cut(s) 212, 1094, 1990, 2216, 2909, 4024, 5108
SpeI ACTAGT 1 cut(s) 2029
SphI GCATGC 2 cut(s) 1824, 4289
SsiI CCGC 3 cut(s) 673, 1028, 3850
SspI AATATT 1 cut(s) 1721
SspMI CTAG 6 cut(s) 1853, 2030, 2667, 3648, 5030, 5119
StyI CCWWGG 8 cut(s) 20, 1468, 3324, 3458, 3647, 3770, 4371, 4707
TaiI ACGT 4 cut(s) 144, 230, 943, 1887
TatI WGTACW 5 cut(s) 186, 1554, 3219, 3564, 4591
TauI GCSGC 1 cut(s) 676
TseFI GTSAC 7 cut(s) 1000, 1604, 1768, 2615, 2973, 3722, 4608
TseI GCWGC 6 cut(s) 1668, 2237, 2240, 2648, 3405, 4697
Tsp45I GTSAC 7 cut(s) 1000, 1604, 1768, 2615, 2973, 3722, 4608
TspGWI ACGGA 4 cut(s) 58, 101, 2494, 2767
TspMI CCCGGG 1 cut(s) 231
Van91I CCANNNNNTGG 2 cut(s) 1815, 2276
VpaK11BI GGWCC 1 cut(s) 333
VspI ATTAAT 1 cut(s) 1334
XceI RCATGY 5 cut(s) 844, 1824, 2237, 2616, 4289
XcmI CCANNNNNNNNNTGG 1 cut(s) 4086
XmaI CCCGGG 1 cut(s) 231
XmaJI CCTAGG 1 cut(s) 3647
XmiI GTMKAC 2 cut(s) 1881, 2004
XmnI GAANNNNTTC 5 cut(s) 162, 942, 2318, 2633, 4740
XspI CTAG 6 cut(s) 1853, 2030, 2667, 3648, 5030, 5119
ZraI GACGTC 2 cut(s) 142, 1885
ZrmI AGTACT 1 cut(s) 188
Zsp2I ATGCAT 5 cut(s) 982, 1511, 3070, 3307, 3701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.