RchiOBHm_Chr7g0189191

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000526
Physical Location & Seq
Forward (+)
8546123 .. 8551483
5361 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16896

Sequence Viewer

Length: 3468 bp
ATGACGCCAAGGGAGCACATAGAAGATATACGGAAGACCAAGTTCTCCATCGGAGAAAAGCTCAACCCTTTAACGGAGGATCTTCACCAGGCCGTCAAGAACCTCTCCGGCGAGCTTTACTCTAAAGACGTCCACTTTTTCATGGAACTCGTTCAGAATGCAGAAGACAATGAGTACTTGGAGGGGGTCGATCCATCCCTTGAGTTTGTGATTACATACCTCGATATAACGGCTACAGGGGCTCCAGCCACATTGCTGGTTTTCAATAATGAGAAGGGGTTTTCTCCCAAAAACATAGACTCCATTTGCAGTGTTGGACGGTCCACCAAGAAAGGCAACAGGAAGCGTGGAATTGGCTTCAAGAGTGTGTTTCTGATCACTGCTCAGCCTTACATATTTAGTAATGGTTATCAGATAAGGTTCAGCGAAGAGCCTTGTTCACATTGCAATGTTGGCTACATAGTACCTGAATGGGTTGAGCAGAATCCCACACTATCTGATATAAAAAAGATATATGGTTCTGCTACTGCCCTTCCTACCACAACACTGATCTTACCTCTGAAGCCCAACAAGGTTGAACCTGTGAAGAAACAACTCTCAATCATTCATCCAGAAGTTCTCTTGTTCCTATCAAAGATTAAGCGGCTCTCTGTTAGGGAAGACAAAGAGGATCCAAGGCAGAACACTGTGAGTGCAATTGAGATAGCAAGCCAGACTGAGTTTGTGTCAAGGAAGAACATCGATGCTCAATCCTACACTCTGCATCTTTCAGCTGAAGAAAATGATGGAAATGGAGAATGCAGTTACTACATGTGGAAACAAAAGTTTCCTGTCAGACAGGAATTCAGAGTTGAGAGGAGAATGGAGGTGGATGAGTGGGTGATCACTCTTGCATTTCCAAATGGAGAACGTCTTCGAAGAGCAAGGAACTCATCGCCTGGTGTTTATGCATTTCTTCCTACAGAGATGGTCACCAACTTTCCCTTCATAATACAGGCTGATTTTCTTTTAGCATCTTCAAGAGAGACAATCCTACTTGACAACAAGTGGAATCAAGGGATTCTTGAGTGTGTGCCCACCGCATTTTCACATGCTTTTAACTCTCTTGTGAAAACTGTGGAAGGTGCGCCAACATCAAGTTTGCCTCCTATGTTCAGATTCTTGCCTGTTCAGGTTTCTTCCTATAAAGAATTAAATGCTGTAAGGGAGTCAATAAAAGCAAAGATGGTTGACGAAGACATTATACCAGTTGAGCCTGAAATGGAGCAGAGGTTCTTCCACAAGCCTCGTGAAGTGGGAAGATTAATGCCTGCCTTTTGGACTATTTTGAGGAAGGCGAGGGTTCAGGGTGTAAGCTTGATTAAGCTTTCATCACATGGAAGATTTGTTCTGTCTTATTCCTTTGACAGCAAGGAATATGATCCTATACTAAGTTTCCTTGGAGTTGAACTAGTGAATGATGCCTGGTATGTTAAATGCATACAGGGTACTTCTGATGTTGTTGATCGAGTGTCAGAAGATGTGTACTTAGAGCTTCTACTGTTTATCGCTGACAATTGGGCTTCTAAATTTGGTCACACCAACATGAAGAACATACCATTGATAAAATATGCAGACTGTAGAAATGAATCTTTATGCAGCATAAGCGAAATCCAGAAAGGTGAATGTAGAGTTTTCTTATCGAGCAAAAGTTCTCATGTTTCATGGCTGATTGATTGCAACCGGGAATTTAATTCTGTGACTCGTCTTCTCTTTATGCCAAAAGCCACTCAGGAAGCTATCTGGGCATGCTCTGGGAAGGATACATTGAAGAAATGGCTAGCTGATCAAGCTAAGGTTGGTTCTGTTGATGTCAATAAATATGCAGTTGATCTACTTCAGAATTCTCTTCATGAGCGGAAGCTTGTTATTGCATATGTTCATTTCTTATATCACTCGGCTGATAATAATTTTATCTCAAGTCGGGAAGTTGACCGTTTATGTGGAATTATGCCACTAGTGGATGGCTATGGGAACATCATCAGAAGTAGAAAAGGCGTTGTTGTTGTTCCTGCCAATGGAAGCAAATGGGCGGGATTGACTGATTCTAATTTGTGGAGAAAAGAAGGCCATGTTGAATTAGGGGAGCATTATATGTATTCTGGCTACTTTTCTGGTAAGTATACACCGGAGAAGAAGCTTCTTGAGTTCCTTATAAAACATGTTGCTGCTTTGGATGTCCCTCATATTTCTGCCCCAAGTGATGGTATCACGTCTGTATCTGCACCACTAACTAAGCAGAATGCCTTCTTGCTTTTGGATTGGATTCGTCAACTGAATTATAAAAGGGTTGGCATCCCTCAGAAGTTCTTGGCTTGCATAAACGAAGGTAGTTGGCTGAAAGTTACTTTGAATGGCTCTCCTGGTTATAGGCCGCCATCCCAGTCATTTCTGCTTAACTCATCATGGGGAAATACTTTGCAGAATGGATCCGTCTCTGTTGACATTCCATTGATTGATACGAGCTTTTATGGTGAGAGCATTAGTGGATACAAGGAGGAGCTAAAGACAATTGGGGTCATGTTTGAATATAGCGAAGCATGTGACTTCATTGGGAAGCATTTCATGTCTCTGGCAGCTTCATCCTCTTTAACTAGAGGAAATGTGCATTCCATTCTGCGTTTCATCAGGTTTTTAAGGGACAAACTTCTTTCTCCGGCCGATTTTATCTCTGCTATTAAGAAAGGGAACTGGCTGAAGACTTCCGTTGGCTACAGGTCTCCTGTAGGATCTGTTTTGTCAGATAAGGAGTGGAATGTTGCCTCCAAAATTAGCAACATTCCCTTCATTGATCAGGACTTTTACGGAGATGAAATCTGTAATTTCAGAACTGAACTTGGGCTGCTTGGTGTTATAATCTCCTTCAACAGAAGTTACCAACTTATAATTGACAACCTGAAGCCACCATCTTGCATAACTTCTTGGACACCTGAGGTTGTTACCTTAATGCTCAACTGTATGGCTCTATCCAAATCATCTGACAAACTTGTCAATGCACTGAAAGGAGTCAAGTGCCTGAAGACAATTATTGGTTACAAGTGTCCAGGGGAATGTCTCTTGTTTCATCACGAATGGGGTTGCATTCTTCAGGTCGTCAATGGTCTTCCCGTGATTGATCATGAATTCTATGGGAGCAACATTTTCCAGTACATAAATGAACTGAAAAAAATTGGTGTGGTGGTTGATTTTGAAGGGGCTGCAAAAGTACTTGCTCGATATTTCAGGCACTATGCAAAATCAGCTTCCATCACCAAGGAAAATGTTGCATCATTGCTTCTGTGTTACAGAGAGTTGAATGGGACTCCATATAAATTTCCTGCTGATATTAGGAGCTGCTTCGCTAAGGTCAAGTGGTTGCGTAGTCGTTTTGGTGATTACAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1155

Amino Acids

131.22

Weight (kDa)

8.49

Isoelectric Point (pI)

37.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 26 - 136 3.8e-08 Sacsin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28020 AT3G48770 AT3G48770
fragaria_vesca FvH4_5g09020 FvH4_5g09020
malus_domestica MD06G1092400.v1.1 MD06G1134600.v1.1 MD06G1134700.v1.1 MD06G1134800.v1.1 MD06G1135200.v1.1 MD06G1135400.v1.1 MD06G1135500.v1.1 MD08G1137600.v1.1 MD15G1395100.v1.1
prunus_persica Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145600_v2.0.a1 Prupe.5G145800_v2.0.a1 Prupe.5G145900_v2.0.a1
pyrus_communis pycom06g12550 pycom06g12570 pycom06g12580 pycom06g12590 pycom06g12610 pycom06g12630 pycom06g12640 pycom06g12650 pycom08g06280
rosa_chinensis RchiOBHm_Chr6g0249321 RchiOBHm_Chr6g0275111 RchiOBHm_Chr7g0189191 RchiOBHm_Chr7g0189261
rosa_laevigata RLG00000004622 RLG00000004623 RLG00000008237 RLG00000008238 RLG00000023303 RLG00000035187
rosa_multiflora Rmu_co8294311.1_g000001 Rmu_co8389833.1_g000001 Rmu_sc0001044.1_g000004 Rmu_sc0001329.1_g000011 Rmu_sc0006012.1_g000010 Rmu_sc0007286.1_g000032 Rmu_sc0009360.1_g000016 Rmu_sc0011817.1_g000001 Rmu_sc0015050.1_g000001
rosa_roxburghii Rroxscaffold_1G00064000 Rroxscaffold_3G00265460 Rroxscaffold_3G00265580 Rroxscaffold_3G00265640 Rroxscaffold_3G00265690 Rroxscaffold_3G00265700 Rroxscaffold_7G00193620 Rroxscaffold_7G00204550 Rroxscaffold_7G00217350
rosa_rugosa Rorug01G0465500 Rorug06G0088500 Rorug06G0496700 Rorug06G0496700
rosa_samantha Rh1AG064800 Rh2BG015100 Rh2BG493500 Rh2DG017300 Rh2DG502500 Rh4BG208500 Rh5BG528500 Rh5CG552900 Rh6CG207800 Rh6DG197900 Rh6DG198000 Rh7AG101300 Rh7AG101400 Rh7AG101600 Rh7BG104100 Rh7BG104200 Rh7CG105600 Rh7CG105700 Rh7DG103200 Rh7DG103400
rosa_wichuraiana Rw1G007300 Rw5G034670 Rw7G008750 Rw7G008780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 2195, 2322, 2894, 2924
AasI GACNNNNNNGTC 1 cut(s) 3026
AatII GACGTC 1 cut(s) 132
AccB7I CCANNNNNTGG 1 cut(s) 2243
AccBSI CCGCTC 1 cut(s) 1897
AccI GTMKAC 1 cut(s) 2162
AciI CCGC 5 cut(s) 643, 1080, 1897, 2072, 2414
AclWI GGATC 8 cut(s) 87, 185, 665, 678, 1415, 2463, 2476, 2776
AcoI YGGCCR 1 cut(s) 2697
AcsI RAATTY 6 cut(s) 842, 1568, 1727, 1882, 3161, 3350
AcuI CTGAAG 7 cut(s) 581, 795, 1862, 2756, 2957, 3077, 3110
AcyI GRCGYC 2 cut(s) 5, 129
AfaI GTAC 6 cut(s) 176, 465, 1489, 1526, 3188, 3246
AfiI CCNNNNNNNGG 3 cut(s) 73, 2057, 2243
AflIII ACRYGT 2 cut(s) 810, 2200
AhlI ACTAGT 2 cut(s) 1450, 1996
AjiI CACGTC 1 cut(s) 2253
AjnI CCWGG 5 cut(s) 87, 937, 1463, 2401, 3082
AjuI GAANNNNNNNTTGG 2 cut(s) 968, 1000
AloI GAACNNNNNNTCC 4 cut(s) 1256, 1288, 1371, 1403
Alw21I GWGCWC 1 cut(s) 18
Alw26I GTCTC 5 cut(s) 1019, 2479, 2613, 2763, 3098
AlwI GGATC 8 cut(s) 87, 185, 665, 678, 1415, 2463, 2476, 2776
AoxI GGCC 4 cut(s) 90, 2107, 2411, 2697
ApeKI GCWGC 6 cut(s) 1638, 2207, 2615, 2881, 3236, 3372
ApoI RAATTY 6 cut(s) 842, 1568, 1727, 1882, 3161, 3350
AseI ATTAAT 1 cut(s) 1304
Asp700I GAANNNNTTC 4 cut(s) 150, 912, 2285, 2600
AspLEI GCGC 1 cut(s) 1129
AspS9I GGNCC 1 cut(s) 321
AsuC2I CCSGG 1 cut(s) 1724
AsuHPI GGTGA 7 cut(s) 77, 892, 964, 1673, 2525, 3280, 3422
AsuII TTCGAA 1 cut(s) 916
AsuNHI GCTAGC 1 cut(s) 1819
AvaII GGWCC 1 cut(s) 321
AxyI CCTNAGG 1 cut(s) 2970
BaeGI GKGCMC 1 cut(s) 1077
BaeI ACNNNNGTAYC 2 cut(s) 2241, 2274
BamHI GGATCC 2 cut(s) 670, 2468
BanII GRGCYC 1 cut(s) 244
BarI GAAGNNNNNNTAC 2 cut(s) 1227, 1259
BauI CACGAG 1 cut(s) 1287
BbsI GAAGAC 9 cut(s) 41, 171, 666, 905, 1242, 1739, 2744, 3065, 3134
Bbv12I GWGCWC 1 cut(s) 18
BbvI GCAGC 6 cut(s) 1650, 2194, 2627, 2868, 3223, 3359
BceAI ACGGC 2 cut(s) 77, 246
BciT130I CCWGG 5 cut(s) 89, 939, 1465, 2403, 3084
BciVI GTATCC 2 cut(s) 1795, 2522
BclI TGATCA 5 cut(s) 375, 882, 1825, 2830, 3154
BcnI CCSGG 1 cut(s) 1724
BcoDI GTCTC 5 cut(s) 1019, 2479, 2613, 2763, 3098
BcuI ACTAGT 2 cut(s) 1450, 1996
BfaI CTAG 4 cut(s) 1451, 1820, 1997, 2634
BfmI CTRYAG 5 cut(s) 234, 960, 1618, 2752, 2763
BfuI GTATCC 2 cut(s) 1795, 2522
BisI GCNGC 8 cut(s) 644, 1639, 2208, 2414, 2616, 2882, 3237, 3373
BlpI GCTNAGC 1 cut(s) 384
BlsI GCNGC 8 cut(s) 645, 1640, 2209, 2415, 2617, 2883, 3238, 3374
BmcAI AGTACT 2 cut(s) 176, 3246
Bme1390I CCNGG 6 cut(s) 89, 939, 1465, 1724, 2403, 3084
Bme18I GGWCC 1 cut(s) 321
BmgBI CACGTC 1 cut(s) 2253
BmgT120I GGNCC 1 cut(s) 321
BmiI GGNNCC 3 cut(s) 243, 672, 2470
BmrFI CCNGG 6 cut(s) 89, 939, 1465, 1724, 2403, 3084
BmrI ACTGGG 1 cut(s) 2416
BmsI GCATC 6 cut(s) 733, 772, 1022, 1450, 2343, 3314
BmtI GCTAGC 1 cut(s) 1823
BmuI ACTGGG 1 cut(s) 2416
BpiI GAAGAC 9 cut(s) 41, 171, 666, 905, 1242, 1739, 2744, 3065, 3134
BplI GAGNNNNNCTC 4 cut(s) 45, 77, 104, 136
BpmI CTGGAG 1 cut(s) 228
Bpu10I CCTNAGC 2 cut(s) 1833, 3381
Bpu1102I GCTNAGC 1 cut(s) 384
Bpu14I TTCGAA 1 cut(s) 916
BpuEI CTTGAG 4 cut(s) 221, 1085, 1942, 2204
BpuMI CCSGG 1 cut(s) 1724
Bsa29I ATCGAT 1 cut(s) 741
BsaHI GRCGYC 2 cut(s) 5, 129
BsaI GGTCTC 1 cut(s) 2763
BsaJI CCNNGG 5 cut(s) 8, 674, 1438, 3083, 3291
BsaWI WCCGGW 1 cut(s) 2167
BsaXI ACNNNNNCTCC 6 cut(s) 226, 256, 284, 314, 1256, 1286
Bsc4I CCNNNNNNNGG 3 cut(s) 73, 2057, 2243
Bse1I ACTGG 4 cut(s) 1247, 2422, 2735, 3184
Bse21I CCTNAGG 1 cut(s) 2970
Bse3DI GCAATG 4 cut(s) 251, 442, 454, 3308
BseBI CCWGG 5 cut(s) 89, 939, 1465, 2403, 3084
BseCI ATCGAT 1 cut(s) 741
BseDI CCNNGG 5 cut(s) 8, 674, 1438, 3083, 3291
BseGI GGATG 8 cut(s) 194, 607, 877, 2008, 2221, 2334, 2417, 2621
BseLI CCNNNNNNNGG 3 cut(s) 73, 2057, 2243
BseMI GCAATG 4 cut(s) 251, 442, 454, 3308
BseMII CTCAG 5 cut(s) 398, 708, 1784, 2354, 2961
BseNI ACTGG 4 cut(s) 1247, 2422, 2735, 3184
BseRI GAGGAG 2 cut(s) 871, 2552
BseSI GKGCMC 1 cut(s) 1077
BseX3I CGGCCG 1 cut(s) 2697
BseXI GCAGC 6 cut(s) 1650, 2194, 2627, 2868, 3223, 3359
BsgI GTGCAG 1 cut(s) 2247
Bsh1285I CGRYCG 1 cut(s) 2700
BshFI GGCC 4 cut(s) 92, 2109, 2413, 2699
BshVI ATCGAT 1 cut(s) 741
BsiEI CGRYCG 1 cut(s) 2700
BsiHKAI GWGCWC 1 cut(s) 18
BsiSI CCGG 4 cut(s) 108, 1723, 2168, 2696
BslFI GGGAC 3 cut(s) 2204, 2693, 3352
BslI CCNNNNNNNGG 3 cut(s) 73, 2057, 2243
BsmAI GTCTC 5 cut(s) 1019, 2479, 2613, 2763, 3098
BsmBI CGTCTC 1 cut(s) 2479
BsmFI GGGAC 3 cut(s) 2204, 2693, 3352
BsmI GAATGC 5 cut(s) 163, 803, 2287, 2647, 3120
BsnI GGCC 4 cut(s) 92, 2109, 2413, 2699
Bso31I GGTCTC 1 cut(s) 2763
Bsp119I TTCGAA 1 cut(s) 916
Bsp1286I GDGCHC 3 cut(s) 18, 244, 1077
Bsp1720I GCTNAGC 1 cut(s) 384
BspACI CCGC 5 cut(s) 643, 1080, 1897, 2072, 2414
BspANI GGCC 4 cut(s) 92, 2109, 2413, 2699
BspCNI CTCAG 5 cut(s) 397, 709, 1783, 2353, 2962
BspDI ATCGAT 1 cut(s) 741
BspHI TCATGA 2 cut(s) 1891, 3157
BspLI GGNNCC 3 cut(s) 243, 672, 2470
BspOI GCTAGC 1 cut(s) 1823
BspPI GGATC 8 cut(s) 87, 185, 665, 678, 1415, 2463, 2476, 2776
BspQI GCTCTTC 2 cut(s) 423, 913
BspT104I TTCGAA 1 cut(s) 916
BspTNI GGTCTC 1 cut(s) 2763
BsrBI CCGCTC 1 cut(s) 1897
BsrDI GCAATG 4 cut(s) 251, 442, 454, 3308
BsrI ACTGG 4 cut(s) 1247, 2422, 2735, 3184
BssECI CCNNGG 5 cut(s) 8, 674, 1438, 3083, 3291
BssNAI GTATAC 1 cut(s) 2163
BssNI GRCGYC 2 cut(s) 5, 129
BssSI CACGAG 1 cut(s) 1287
BssT1I CCWWGG 4 cut(s) 8, 674, 1438, 3291
Bst1107I GTATAC 1 cut(s) 2163
Bst2BI CACGAG 1 cut(s) 1287
Bst2UI CCWGG 5 cut(s) 89, 939, 1465, 2403, 3084
Bst4CI ACNGT 7 cut(s) 321, 688, 1117, 1542, 1619, 1976, 2996
Bst6I CTCTTC 3 cut(s) 423, 913, 1893
BstACI GRCGYC 2 cut(s) 5, 129
BstBI TTCGAA 1 cut(s) 916
BstC8I GCNNGC 6 cut(s) 113, 709, 1311, 1789, 1821, 2356
BstEII GGTNACC 1 cut(s) 970
BstF5I GGATG 8 cut(s) 194, 607, 877, 2008, 2221, 2334, 2417, 2621
BstHHI GCGC 1 cut(s) 1129
BstMAI GTCTC 5 cut(s) 1019, 2479, 2613, 2763, 3098
BstMCI CGRYCG 1 cut(s) 2700
BstMWI GCNNNNNNNGC 7 cut(s) 13, 239, 453, 1644, 1784, 1829, 3278
BstNI CCWGG 5 cut(s) 89, 939, 1465, 2403, 3084
BstNSI RCATGY 5 cut(s) 814, 1094, 1791, 2204, 2583
BstPI GGTNACC 1 cut(s) 970
BstSCI CCNGG 6 cut(s) 87, 937, 1463, 1722, 2401, 3082
BstSFI CTRYAG 5 cut(s) 234, 960, 1618, 2752, 2763
BstSLI GKGCMC 1 cut(s) 1077
BstV1I GCAGC 6 cut(s) 1650, 2194, 2627, 2868, 3223, 3359
BstV2I GAAGAC 9 cut(s) 41, 171, 666, 905, 1242, 1739, 2744, 3065, 3134
BstX2I RGATCY 4 cut(s) 79, 670, 2468, 2768
BstXI CCANNNNNNTGG 1 cut(s) 256
BstYI RGATCY 4 cut(s) 79, 670, 2468, 2768
BstZ17I GTATAC 1 cut(s) 2163
BstZI CGGCCG 1 cut(s) 2697
Bsu15I ATCGAT 1 cut(s) 741
Bsu36I CCTNAGG 1 cut(s) 2970
BsuI GTATCC 2 cut(s) 1795, 2522
BsuRI GGCC 4 cut(s) 92, 2109, 2413, 2699
BsuTUI ATCGAT 1 cut(s) 741
BtgZI GCGATG 1 cut(s) 918
BtrI CACGTC 1 cut(s) 2253
BtsCI GGATG 8 cut(s) 194, 607, 877, 2008, 2221, 2334, 2417, 2621
BtsI GCAGTG 2 cut(s) 316, 378
BtsIMutI CAGTG 5 cut(s) 316, 378, 545, 684, 3035
Cac8I GCNNGC 6 cut(s) 113, 709, 1311, 1789, 1821, 2356
CciI TCATGA 2 cut(s) 1891, 3157
CfoI GCGC 1 cut(s) 1129
Cfr13I GGNCC 1 cut(s) 321
ClaI ATCGAT 1 cut(s) 741
CseI GACGC 1 cut(s) 13
Csp6I GTAC 6 cut(s) 175, 464, 1488, 1525, 3187, 3245
CviQI GTAC 6 cut(s) 175, 464, 1488, 1525, 3187, 3245
DrdI GACNNNNNNGTC 1 cut(s) 3026
DseDI GACNNNNNNGTC 1 cut(s) 3026
EaeI YGGCCR 1 cut(s) 2697
EagI CGGCCG 1 cut(s) 2697
Eam1104I CTCTTC 3 cut(s) 423, 913, 1893
EarI CTCTTC 3 cut(s) 423, 913, 1893
EclXI CGGCCG 1 cut(s) 2697
Eco130I CCWWGG 4 cut(s) 8, 674, 1438, 3291
Eco24I GRGCYC 1 cut(s) 244
Eco31I GGTCTC 1 cut(s) 2763
Eco47I GGWCC 1 cut(s) 321
Eco52I CGGCCG 1 cut(s) 2697
Eco57I CTGAAG 7 cut(s) 581, 795, 1862, 2756, 2957, 3077, 3110
Eco81I CCTNAGG 1 cut(s) 2970
Eco91I GGTNACC 1 cut(s) 970
EcoO65I GGTNACC 1 cut(s) 970
EcoRI GAATTC 3 cut(s) 842, 1882, 3161
EcoRII CCWGG 5 cut(s) 87, 937, 1463, 2401, 3082
EcoT14I CCWWGG 4 cut(s) 8, 674, 1438, 3291
EcoT22I ATGCAT 2 cut(s) 952, 1481
EcoT38I GRGCYC 1 cut(s) 244
ErhI CCWWGG 4 cut(s) 8, 674, 1438, 3291
Esp3I CGTCTC 1 cut(s) 2479
FalI AAGNNNNNCTT 2 cut(s) 1047, 1079
FaqI GGGAC 3 cut(s) 2204, 2693, 3352
FauI CCCGC 1 cut(s) 2065
FauNDI CATATG 1 cut(s) 1915
FbaI TGATCA 5 cut(s) 375, 882, 1825, 2830, 3154
FblI GTMKAC 1 cut(s) 2162
Fnu4HI GCNGC 8 cut(s) 644, 1639, 2208, 2414, 2616, 2882, 3237, 3373
FokI GGATG 8 cut(s) 181, 594, 884, 2015, 2228, 2321, 2404, 2608
FriOI GRGCYC 1 cut(s) 244
Fsp4HI GCNGC 8 cut(s) 644, 1639, 2208, 2414, 2616, 2882, 3237, 3373
FspBI CTAG 4 cut(s) 1451, 1820, 1997, 2634
GlaI GCGC 1 cut(s) 1128
GluI GCNGC 8 cut(s) 644, 1639, 2208, 2414, 2616, 2882, 3237, 3373
GsuI CTGGAG 1 cut(s) 228
HaeIII GGCC 4 cut(s) 92, 2109, 2413, 2699
HapII CCGG 4 cut(s) 108, 1723, 2168, 2696
HgaI GACGC 1 cut(s) 13
HhaI GCGC 1 cut(s) 1129
Hin1I GRCGYC 2 cut(s) 5, 129
Hin6I GCGC 1 cut(s) 1127
HinP1I GCGC 1 cut(s) 1127
HincII GTYRAC 4 cut(s) 1231, 1972, 2312, 2482
HindII GTYRAC 4 cut(s) 1231, 1972, 2312, 2482
HindIII AAGCTT 4 cut(s) 1354, 1364, 1901, 2177
HpaII CCGG 4 cut(s) 108, 1723, 2168, 2696
HphI GGTGA 7 cut(s) 77, 892, 964, 1673, 2525, 3280, 3422
Hpy166II GTNNAC 9 cut(s) 133, 324, 440, 1231, 1525, 1972, 2163, 2312, 2482
Hpy8I GTNNAC 9 cut(s) 133, 324, 440, 1231, 1525, 1972, 2163, 2312, 2482
HpyCH4III ACNGT 7 cut(s) 321, 688, 1117, 1542, 1619, 1976, 2996
HpyCH4IV ACGT 3 cut(s) 129, 910, 2252
HpyF10VI GCNNNNNNNGC 7 cut(s) 13, 239, 453, 1644, 1784, 1829, 3278
HpySE526I ACGT 3 cut(s) 129, 910, 2252
Hsp92I GRCGYC 2 cut(s) 5, 129
HspAI GCGC 1 cut(s) 1127
Ksp22I TGATCA 5 cut(s) 375, 882, 1825, 2830, 3154
LguI GCTCTTC 2 cut(s) 423, 913
LmnI GCTCC 7 cut(s) 13, 247, 1264, 2125, 2539, 3171, 3369
Lsp1109I GCAGC 6 cut(s) 1650, 2194, 2627, 2868, 3223, 3359
LweI GCATC 6 cut(s) 733, 772, 1022, 1450, 2343, 3314
MaeI CTAG 4 cut(s) 1451, 1820, 1997, 2634
MaeII ACGT 3 cut(s) 129, 910, 2252
MbiI CCGCTC 1 cut(s) 1897
MfeI CAATTG 3 cut(s) 696, 1555, 2550
MflI RGATCY 4 cut(s) 79, 670, 2468, 2768
MhlI GDGCHC 3 cut(s) 18, 244, 1077
MlyI GAGTC 5 cut(s) 293, 1217, 1735, 3054, 3334
MmeI TCCRAC 1 cut(s) 295
Mph1103I ATGCAT 2 cut(s) 952, 1481
MroXI GAANNNNTTC 4 cut(s) 150, 912, 2285, 2600
MslI CAYNNNNRTG 2 cut(s) 447, 1583
MspA1I CMGCKG 1 cut(s) 773
MspI CCGG 4 cut(s) 108, 1723, 2168, 2696
MspR9I CCNGG 6 cut(s) 89, 939, 1465, 1724, 2403, 3084
MunI CAATTG 3 cut(s) 696, 1555, 2550
Mva1269I GAATGC 5 cut(s) 163, 803, 2287, 2647, 3120
MvaI CCWGG 5 cut(s) 89, 939, 1465, 2403, 3084
MwoI GCNNNNNNNGC 7 cut(s) 13, 239, 453, 1644, 1784, 1829, 3278
NciI CCSGG 1 cut(s) 1724
NdeI CATATG 1 cut(s) 1915
NheI GCTAGC 1 cut(s) 1819
NlaIV GGNNCC 3 cut(s) 243, 672, 2470
NmeAIII GCCGAG 1 cut(s) 1916
NmuCI GTSAC 4 cut(s) 970, 1574, 1738, 2582
NsiI ATGCAT 2 cut(s) 952, 1481
NspI RCATGY 5 cut(s) 814, 1094, 1791, 2204, 2583
NspV TTCGAA 1 cut(s) 916
PaeI GCATGC 1 cut(s) 1791
PagI TCATGA 2 cut(s) 1891, 3157
PciI ACATGT 2 cut(s) 810, 2200
PciSI GCTCTTC 2 cut(s) 423, 913
PctI GAATGC 5 cut(s) 163, 803, 2287, 2647, 3120
PdmI GAANNNNTTC 4 cut(s) 150, 912, 2285, 2600
PfeI GAWTC 7 cut(s) 484, 1051, 1060, 1158, 1628, 2084, 2305
PflMI CCANNNNNTGG 1 cut(s) 2243
PkrI GCNGC 8 cut(s) 645, 1640, 2209, 2415, 2617, 2883, 3238, 3374
PleI GAGTC 5 cut(s) 293, 1216, 1735, 3053, 3334
PpsI GAGTC 5 cut(s) 293, 1216, 1735, 3053, 3334
PscI ACATGT 2 cut(s) 810, 2200
PshBI ATTAAT 1 cut(s) 1304
PsiI TTATAA 4 cut(s) 2195, 2322, 2894, 2924
Psp6I CCWGG 5 cut(s) 87, 937, 1463, 2401, 3082
PspEI GGTNACC 1 cut(s) 970
PspGI CCWGG 5 cut(s) 87, 937, 1463, 2401, 3082
PspN4I GGNNCC 3 cut(s) 243, 672, 2470
PspPI GGNCC 1 cut(s) 321
PsuI RGATCY 4 cut(s) 79, 670, 2468, 2768
PvuII CAGCTG 1 cut(s) 773
RsaI GTAC 6 cut(s) 176, 465, 1489, 1526, 3188, 3246
RsaNI GTAC 6 cut(s) 175, 464, 1488, 1525, 3187, 3245
RseI CAYNNNNRTG 2 cut(s) 447, 1583
SapI GCTCTTC 2 cut(s) 423, 913
SatI GCNGC 8 cut(s) 644, 1639, 2208, 2414, 2616, 2882, 3237, 3373
Sau96I GGNCC 1 cut(s) 321
ScaI AGTACT 2 cut(s) 176, 3246
SchI GAGTC 5 cut(s) 293, 1217, 1735, 3054, 3334
ScrFI CCNGG 6 cut(s) 89, 939, 1465, 1724, 2403, 3084
SduI GDGCHC 3 cut(s) 18, 244, 1077
SfaNI GCATC 6 cut(s) 733, 772, 1022, 1450, 2343, 3314
SfcI CTRYAG 5 cut(s) 234, 960, 1618, 2752, 2763
SfuI TTCGAA 1 cut(s) 916
SinI GGWCC 1 cut(s) 321
SmiMI CAYNNNNRTG 2 cut(s) 447, 1583
SmlI CTYRAG 4 cut(s) 200, 1064, 1957, 2183
SmoI CTYRAG 4 cut(s) 200, 1064, 1957, 2183
SpeI ACTAGT 2 cut(s) 1450, 1996
SphI GCATGC 1 cut(s) 1791
SsiI CCGC 5 cut(s) 643, 1080, 1897, 2072, 2414
SspMI CTAG 4 cut(s) 1451, 1820, 1997, 2634
StyD4I CCNGG 6 cut(s) 87, 937, 1463, 1722, 2401, 3082
StyI CCWWGG 4 cut(s) 8, 674, 1438, 3291
TaaI ACNGT 7 cut(s) 321, 688, 1117, 1542, 1619, 1976, 2996
TaiI ACGT 3 cut(s) 132, 913, 2255
TaqI TCGA 7 cut(s) 189, 222, 741, 916, 1507, 1682, 3253
TatI WGTACW 4 cut(s) 174, 1524, 3186, 3244
TauI GCSGC 2 cut(s) 646, 2416
TfiI GAWTC 7 cut(s) 484, 1051, 1060, 1158, 1628, 2084, 2305
TscAI CASTG 5 cut(s) 316, 385, 552, 691, 3042
TseFI GTSAC 4 cut(s) 970, 1574, 1738, 2582
TseI GCWGC 6 cut(s) 1638, 2207, 2615, 2881, 3236, 3372
Tsp45I GTSAC 4 cut(s) 970, 1574, 1738, 2582
TspGWI ACGGA 5 cut(s) 46, 89, 2461, 2734, 2859
TspRI CASTG 5 cut(s) 316, 385, 552, 691, 3042
Van91I CCANNNNNTGG 1 cut(s) 2243
VpaK11BI GGWCC 1 cut(s) 321
VspI ATTAAT 1 cut(s) 1304
XapI RAATTY 6 cut(s) 842, 1568, 1727, 1882, 3161, 3350
XceI RCATGY 5 cut(s) 814, 1094, 1791, 2204, 2583
XmiI GTMKAC 1 cut(s) 2162
XmnI GAANNNNTTC 4 cut(s) 150, 912, 2285, 2600
XspI CTAG 4 cut(s) 1451, 1820, 1997, 2634
ZraI GACGTC 1 cut(s) 130
ZrmI AGTACT 2 cut(s) 176, 3246
Zsp2I ATGCAT 2 cut(s) 952, 1481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.