Rroxscaffold_3G00265580

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000526
Physical Location & Seq
Reverse (-)
58971855 .. 58978345
6491 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00265580.1

Sequence Viewer

Length: 5106 bp
ATGGCGGCGACGATGACGCCAAGGGAGCACATAGAAGATATACGGAAGACCAAGTTCTCCATCGGAGAAAAGCTCAACCCTTTAACGGAGGATCTTCACCAGGCCGTCAAGAACCTCTCCGGCGAGCTTTACGCTAAAGACGTCCACTTTTTCATGGAACTCGTTCAGAATGCAGAAGACAATGAGTACTCAGAGGGGGTTGATCCATCCCTTGAGTTTTTGATTACGTCCCGGGATATAACGGCTACAGGTGCTCCAGCCACATTGCTGGTTTTCAATAATGAGAAGGGGTTTTCTTCCAAAAACATAGACTCCATTTGCAGTGTTGGACGGTCCACCAAGAAAGGCAACAGGAAGCGTGGTTATATTGGGGAGAAAGGAATTGGCTTCAAGAGTGTGTTTCTGATCACTGCTCAGCCTTACATTTTTAGTAATGGTTATCAGATAAGGTTTAGCGAAGAGCCTTGTTCACATTGCAATGTTGGCTACATAGTACCTGAATGGGTTGAGCAGAATCCCACACTGTCTGATATAAAAAAGATATATGGTTCTGCTACTGCCCTTCCTACCACAACACTGATCTTACCTCTGAAGCCCGACAAGGTCGAACCTGTGAAGAACCAACTCTCAATCATTCATCCAGAAGTTCTCTTGTTCCTATCAAAGATTAAGCGGCTCTCTGTTAGGCAAGACAAAGAGGATCCAAGGAAGAACACTGTGAGTGCAATTGAGATAGCAAGCCAGACTGATTTTGTGGAAAGGAAGAACATCGATGCTCAATCCTACACTCTGCGTCTTTCAGCTGAAGAAAATGATGAAAATGGAGAATGCAATTATTACATGTGGAAACAAAAGTTTCCTGTCAGGCAGGAATTCAGAGTTGAGAGGAGAATGGAGGTGGATGAGTGGGTGATCACTCTTGCATTTCCAAATGGAGAACGTCTTCGAAGAGCAAGGAACTCTTCGCCTGGTGTTTATGCATTTCTTCCCACAGAGATGGTCACCAACTTTCCATTCATAATACAGGCGGATTTTCTCTTAGCATCTTCAAGAGAGACAATCCTACTTGACAACAAGTGGAATCAAGGGATTCTTGAGTGTGTGCCCACCGCATTTGCCCATGCCTTTAACTCCTTTGTGAAAACTGTGGTAGGTGCGCCAGCATCAAGCTTGGCTTCTAATTTCAGATTCTTGCCTGTTCAGGGTTCTTCCTATAAAGAATTAAATGCTGTAAGGGAGTCCATACAAGCGAAGATGGTTGAGGAAGACATTATACCAGTTGAGCCTGAAATGGAGCAGAGGTTCTTCCACAAGCCTCGTGAAGTGGGAAGATTAATGCCTGCCTTTTGGTCTATTTTGAGGAAGGCGAGGGTTCAAGGTGTAAGCTGGATTAAGCTTTCATCACATGGAAGATTTATTCTGTCTTATTCCTTTGACAGTGAGGAATATGATCCTATACTAAGTTTCCTTGGAGTTGAACCAGTTAAAGATGATTGGTATGTTAAATGCATACAGGGTACTTCTAATCTTGTTGATGGAGTGTCAGAAGATGTGTACTTAGAGCTTCTACTGTTTATCGCTGACAATTGGGGTTCTAAATTTGGTCACACCAACATGAAGAACATACCATTGATAAAATATGCGGACTATGGAAATGAATCTTTATGCAGCATAAGCGAAATCCAGAAAGGTGAATGTAGAGTTTGCTTATCGAGCAAATATTCTCGTGTTTCATGGCTGATTGATTGCAACCGGGAATTTAATTCTGTGACTCGTCTTCTCTTTATGCCGAAAGCCACTCAGGAAGCCATCTGGACATGCTCCGGGAAGGATACATTGAAGAAATGGCTAGCTGATCAAGCTAAGGTTGGTTCTGTTGATGTCAATCTATATGCAGTTGATCTCCTCCTATATTCCCTTAATGAGCGGAAGCTTGTTATTGCTTATGTTCACTTCTTATATCAGTCATTCAACAATGATTTTCTCTCTAGTGGGGAAGTTGATAATATATGTAAAAGTATGCCACTAGTGGATGGCTACGGGGACATCGTCAGAAGTAGAAACGGGGTTATTGTTCCTGCCAATGGAAGCAAATGGGCGGGATTGACTGATTCTAATTTGTGGAGAAAAGACGGCTATGTTGAATTAGGAGAGCATTATATGAATTCTGGCTATTTTGCTGGTAAGTATACACCGCGGAAAAAGCTTCTTGAGTTCCTTGTAGAACATGCTGCTGCTATGGATGTCCCTCATATTTCTGCTCCAAGTGATGGAATCTCGTCTGTATCTGCACCACTAACTAAGCAGAATGCCTTCTTGCTTTTGGATTGGATTCGTCAACTGAATTATAAAAGGGTTGGCATCCCTCAGAAGTTCTTGGCTTGCATAAAGGAAGGTAGTTGGCTGAAAGTTACTTTGAATGGCTCTCCTGGTTATAGGCCACCATCCCAGTCATTTCTGCTTAACTCATCATGGGGAAATACTTTGCAGAATGGATCCGTCTCTGTTGACATTCCATTGATTGATACGAGCTTTTATGGTGAGACCATTAGTGAATACAAGGAGGAGCTAAAGACAATTGGGGTCATGTTTGAATATAGCGAAGCATGTGACTTCATTGGGAAGCATTTCATGTCTCTGGCAGCTTCATCCTCTTTAACTAGAGGAAATGTGCATTCCATTCTGCGTTTCATCAGGTTTTTAAGGGACAAACTTCTTTCTCCGGCCGATTTTATCACTGCTATTAAAAAAGGGAACTGGCTGAAGACTTCCGTTGGCTACAGGTCTCCTGTAGGATCTGTTTTGTCAGGTAAGGAGTGGAATGTTGCCTCCAAAATTAGCAACATTCCCTTCATTGATCAGGACTTTTACGGTGATGAAATCTGTAATTTCAGAACTGAACTTGAGCTGCTTGGTGTTGTAATCTCCTTCAACAGAAGTTACCAACTTATAATTGACAACCTGAAGTTACCATCTTCCATAACTTCTTGGACACCTCAGGTTGTCATCTTAATGCTCAACTGTATGGCTCTATCCAAGTCCTCTGATAAACTTGTCAATGCATTGAAAGGAGCCAAGTGCCTGAAGACAAGTGTTGGTTACAAGTATCCAGGGGAATGTCTCTTGTTTCATCACGAATGGGGTTGCATTCTTCGGGTCGTCAATGGTCTTCCCGTGATTGATCATGAATTCTATGGGAGCAACATTTTCCGGTACATAAATGAACTGAAAAAAATTGGTGTGGTGGTTGATTTTGAAGAGGCTGCAAAAGTATTTGCTCGATATTTCAGGCACTATGCATCATCAGCTTCCATCACCAAGGAAAATGTTGCATCATTGCTTCTGTGTTACAGAGCGCTGAAGGGGACTCAATATAAATTTCCTGCTGATCTTAGGAGCTGCATTGGTGAGGTCAAGTGGTTGCGTAGTCGTTTTGGTGATTACAGACGTCCAAGGGAGTGCATTCTCTTTAGTCCCGAGTGGGAATCTATATCCCCAATAACTATGCTTCCTTTCATTGATGATTCTGACAGTTACTATGGAAAGGGCATACATGAGTACAGAAAAGAGCTGAAGAGCTTGGGGGTTGTCGTCGAATACAAGGAAGGAGTTAATTTTGTGGCTTCTGGTCTTTATCTCCCTAGGGATGCCAGCCGTATTTTTCCTGTAAATGCACTTGCGTTGCTGGAATGCATACGAGTTTCCCATGAGAAAGGTTACGCCTTGCTTGATAATTCTGATTTCATGAAGAAAGTTTCTCAACCATGGATAAAGACTCATGCTGGTTATAGGTCTCCCAAAGAGTCTTTGTTGTTTGATTCCAAGTTTGGCTTATATTTGAAGCGGACTGATGGGCCTTTCATTGATGAAGAATTCTATGGTACTAAGATTGCATCTTACAAAAAAGAGCTGTCTGCATTGGAGGTTGTTGTTGAAGCTGAGAAAGGATGTCCCCTGGTTGCCGGCCACCTTGCTGTTCATTATGGGATTAAAGCATTTGCTCGGCTGTATAATTACTTGAGTGCATTCAAGTGGGAACCAGACACTGATGCTGACAGATGGATCTGGGTTCCAAAAGGAGAGTGGGTTCGTCCTGATGATTGTGTTATATATGACAAGGATGGGCTCTTTGGTTTACAATTGACTGTTTTGGAGAAACATTATGAGCAGAAACTTCTATTTTTCTTCTCCAGTGCGTTTAGAGTTAAATCTCATCCTTCAGTTGATGATTACTTGAAGGTTTGGAATGTTTGGGAAAGTTCTGGAAGTGCATTGTCGCATGCTGATTGTTGCAAGTTTTGGAGTTATATCTCGAAGCACTGGAATTCAAAGACAGAAAAGACTGTTGCTGATGCCTTGGTGAAAGTACCTGTCAGTTCTGGATCTGAAGGAATCTTGCTGTGCAACAAGTCTGATGTTTTCATTGCTGATGACCTTCAACTGAAGTATCTATTCGAACAGTCTTCTCATGAGTCTATTTTTGTCTGGTACCCTCAGCCAAGTTTGCCTTCCATTCCAAGATCCAAGTTGCTTGACATTTATCGAAGAATTGGTGTTCGCACTATATCTGAATCTGTACAGAAGGAAGAGGCGTCACTTGCAGATGATGTTGAAGTAGTCTCTTTGCCGAGGGAAAAGCTGATTAAGAAAGCGCTGTTCAGGCTGATTCTTGGTTTTCTTGCTGCTCCTCCCATGGAAATGGAAGCTGAGAAGAGGCTTGAAGCTGTTCAATTCCTTGTGAATGTTACTGTGGTGGTAACAGCTGAACCAATCTCTGTAAGCTATAAGCTACCTTTATCTTCTGGGAAAGTTTTGAATGTGAGAGGAAGCCGAAAAATACGGTTTGATAGAGAAAATTCAAAGATGTTCACTCAGAAAATGGACAAGTCTGGAGGGCAGAAGAGTATGATTGAGTTTGCTACTTTTTTCTCTGAAGTGATATCTGAGAGTGTGTTATGGGAGAGCACTGATCATATAGATTCACTCTCTGAGCTGATCAAGATGGCTTCTTTGCTAGAGTTCAATGAGGAAGCAGTTGATTTCCTGATGAATTCCAAGAATTTGCAAACTTTTATGGAGGATGAGGAGTTTCTCAAGACTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1701

Amino Acids

193.05

Weight (kDa)

6.71

Isoelectric Point (pI)

39.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 30 - 146 1.9e-11 Sacsin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28020 AT3G48770 AT3G48770
fragaria_vesca FvH4_5g09020 FvH4_5g09020
malus_domestica MD06G1092400.v1.1 MD06G1134600.v1.1 MD06G1134700.v1.1 MD06G1134800.v1.1 MD06G1135200.v1.1 MD06G1135400.v1.1 MD06G1135500.v1.1 MD08G1137600.v1.1 MD15G1395100.v1.1
prunus_persica Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145600_v2.0.a1 Prupe.5G145800_v2.0.a1 Prupe.5G145900_v2.0.a1
pyrus_communis pycom06g12550 pycom06g12570 pycom06g12580 pycom06g12590 pycom06g12610 pycom06g12630 pycom06g12640 pycom06g12650 pycom08g06280
rosa_chinensis RchiOBHm_Chr6g0249321 RchiOBHm_Chr6g0275111 RchiOBHm_Chr7g0189191 RchiOBHm_Chr7g0189261
rosa_laevigata RLG00000004622 RLG00000004623 RLG00000008237 RLG00000008238 RLG00000023303 RLG00000035187
rosa_multiflora Rmu_co8294311.1_g000001 Rmu_co8389833.1_g000001 Rmu_sc0001044.1_g000004 Rmu_sc0001329.1_g000011 Rmu_sc0006012.1_g000010 Rmu_sc0007286.1_g000032 Rmu_sc0009360.1_g000016 Rmu_sc0011817.1_g000001 Rmu_sc0015050.1_g000001
rosa_roxburghii Rroxscaffold_1G00064000 Rroxscaffold_3G00265460 Rroxscaffold_3G00265580 Rroxscaffold_3G00265640 Rroxscaffold_3G00265690 Rroxscaffold_3G00265700 Rroxscaffold_7G00193620 Rroxscaffold_7G00204550 Rroxscaffold_7G00217350
rosa_rugosa Rorug01G0465500 Rorug06G0088500 Rorug06G0496700 Rorug06G0496700
rosa_samantha Rh1AG064800 Rh2BG015100 Rh2BG493500 Rh2DG017300 Rh2DG502500 Rh4BG208500 Rh5BG528500 Rh5CG552900 Rh6CG207800 Rh6DG197900 Rh6DG198000 Rh7AG101300 Rh7AG101400 Rh7AG101600 Rh7BG104100 Rh7BG104200 Rh7CG105600 Rh7CG105700 Rh7DG103200 Rh7DG103400
rosa_wichuraiana Rw1G007300 Rw5G034670 Rw7G008750 Rw7G008780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2349, 2951
AatII GACGTC 2 cut(s) 144, 3451
Acc65I GGTACC 1 cut(s) 4488
AccB1I GGYRCC 1 cut(s) 4488
AccB7I CCANNNNNTGG 1 cut(s) 2270
AccBSI CCGCTC 1 cut(s) 1927
AccI GTMKAC 1 cut(s) 2189
AccII CGCG 1 cut(s) 2197
AcoI YGGCCR 2 cut(s) 2724, 3964
AcyI GRCGYC 4 cut(s) 17, 141, 3448, 4592
AfeI AGCGCT 2 cut(s) 3357, 4653
AfiI CCNNNNNNNGG 4 cut(s) 85, 1202, 2084, 2270
AflIII ACRYGT 1 cut(s) 840
AhlI ACTAGT 1 cut(s) 2026
AjnI CCWGG 5 cut(s) 99, 967, 2428, 3109, 3954
AjuI GAANNNNNNNTTGG 4 cut(s) 998, 1030, 4492, 4524
AloI GAACNNNNNNTCC 4 cut(s) 1286, 1318, 4071, 4103
Alw21I GWGCWC 3 cut(s) 30, 256, 4967
Alw26I GTCTC 8 cut(s) 1049, 2506, 2537, 2640, 2790, 3125, 3798, 4624
AlwNI CAGNNNCTG 1 cut(s) 4046
Ama87I CYCGRG 2 cut(s) 231, 3476
Aor51HI AGCGCT 2 cut(s) 3357, 4653
AoxI GGCC 5 cut(s) 102, 2438, 2724, 3854, 3964
ApeKI GCWGC 8 cut(s) 1668, 2231, 2234, 2642, 2908, 3263, 3399, 4682
AseI ATTAAT 1 cut(s) 1334
Asp700I GAANNNNTTC 5 cut(s) 162, 942, 2312, 2627, 4725
Asp718I GGTACC 1 cut(s) 4488
AspA2I CCTAGG 1 cut(s) 3641
AspLEI GCGC 3 cut(s) 1159, 3358, 4654
AspS9I GGNCC 2 cut(s) 333, 3854
AsuC2I CCSGG 4 cut(s) 232, 233, 1754, 1825
AsuII TTCGAA 2 cut(s) 946, 4455
AsuNHI GCTAGC 1 cut(s) 1849
AvaI CYCGRG 2 cut(s) 231, 3476
AvaII GGWCC 1 cut(s) 333
AvrII CCTAGG 1 cut(s) 3641
AxyI CCTNAGG 1 cut(s) 2997
BaeGI GKGCMC 1 cut(s) 1107
BaeI ACNNNNGTAYC 2 cut(s) 4430, 4463
BamHI GGATCC 2 cut(s) 700, 2495
BanI GGYRCC 1 cut(s) 4488
BanII GRGCYC 1 cut(s) 4128
BarI GAAGNNNNNNTAC 2 cut(s) 1257, 1289
BauI CACGAG 2 cut(s) 1317, 1725
BbsI GAAGAC 9 cut(s) 53, 183, 935, 1272, 1769, 2771, 3092, 3161, 4455
Bbv12I GWGCWC 3 cut(s) 30, 256, 4967
BbvCI CCTCAGC 1 cut(s) 4494
BbvI GCAGC 8 cut(s) 1680, 2218, 2221, 2654, 2895, 3250, 3386, 4669
BceAI ACGGC 4 cut(s) 89, 258, 2149, 3639
BciT130I CCWGG 5 cut(s) 101, 969, 2430, 3111, 3956
BciVI GTATCC 2 cut(s) 1825, 3117
BclI TGATCA 7 cut(s) 405, 912, 1855, 2857, 3181, 4969, 4995
BcnI CCSGG 4 cut(s) 232, 233, 1754, 1825
BcoDI GTCTC 8 cut(s) 1049, 2506, 2537, 2640, 2790, 3125, 3798, 4624
BcuI ACTAGT 1 cut(s) 2026
BfaI CTAG 7 cut(s) 1850, 1989, 2027, 2661, 3642, 5015, 5104
BfmI CTRYAG 3 cut(s) 246, 2779, 2790
BfoI RGCGCY 2 cut(s) 3359, 4655
BfuI GTATCC 2 cut(s) 1825, 3117
BlnI CCTAGG 1 cut(s) 3641
BlpI GCTNAGC 1 cut(s) 414
BmcAI AGTACT 1 cut(s) 188
Bme1390I CCNGG 9 cut(s) 101, 232, 233, 969, 1754, 1825, 2430, 3111, 3956
Bme18I GGWCC 1 cut(s) 333
BmeT110I CYCGRG 2 cut(s) 231, 3476
BmgT120I GGNCC 2 cut(s) 333, 3854
BmiI GGNNCC 6 cut(s) 702, 2497, 3073, 4038, 4071, 4490
BmrFI CCNGG 9 cut(s) 101, 232, 233, 969, 1754, 1825, 2430, 3111, 3956
BmrI ACTGGG 1 cut(s) 2443
BmtI GCTAGC 1 cut(s) 1853
BmuI ACTGGG 1 cut(s) 2443
BpiI GAAGAC 9 cut(s) 53, 183, 935, 1272, 1769, 2771, 3092, 3161, 4455
BplI GAGNNNNNCTC 2 cut(s) 57, 89
BpmI CTGGAG 3 cut(s) 240, 4174, 4911
Bpu10I CCTNAGC 2 cut(s) 1863, 4494
Bpu1102I GCTNAGC 1 cut(s) 414
Bpu14I TTCGAA 2 cut(s) 946, 4455
BpuEI CTTGAG 6 cut(s) 233, 1115, 2231, 2924, 4039, 5078
BpuMI CCSGG 4 cut(s) 232, 233, 1754, 1825
Bsa29I ATCGAT 1 cut(s) 771
BsaBI GATNNNNATC 1 cut(s) 1884
BsaHI GRCGYC 4 cut(s) 17, 141, 3448, 4592
BsaI GGTCTC 3 cut(s) 2537, 2790, 3798
BsaWI WCCGGW 1 cut(s) 3210
BsaXI ACNNNNNCTCC 8 cut(s) 238, 268, 296, 326, 1286, 1316, 4071, 4101
Bsc4I CCNNNNNNNGG 4 cut(s) 85, 1202, 2084, 2270
Bse118I RCCGGY 1 cut(s) 3962
Bse1I ACTGG 6 cut(s) 1277, 1481, 2449, 2762, 4191, 4325
Bse21I CCTNAGG 1 cut(s) 2997
Bse3DI GCAATG 5 cut(s) 263, 472, 484, 3335, 4422
Bse8I GATNNNNATC 1 cut(s) 1884
BseBI CCWGG 5 cut(s) 101, 969, 2430, 3111, 3956
BseCI ATCGAT 1 cut(s) 771
BseJI GATNNNNATC 1 cut(s) 1884
BseLI CCNNNNNNNGG 4 cut(s) 85, 1202, 2084, 2270
BseMI GCAATG 5 cut(s) 263, 472, 484, 3335, 4422
BseNI ACTGG 6 cut(s) 1277, 1481, 2449, 2762, 4191, 4325
BseRI GAGGAG 5 cut(s) 901, 1895, 2579, 4677, 5099
BseSI GKGCMC 1 cut(s) 1107
BseX3I CGGCCG 1 cut(s) 2724
BseXI GCAGC 8 cut(s) 1680, 2218, 2221, 2654, 2895, 3250, 3386, 4669
BsgI GTGCAG 1 cut(s) 2274
Bsh1236I CGCG 1 cut(s) 2197
Bsh1285I CGRYCG 1 cut(s) 2727
BshFI GGCC 5 cut(s) 104, 2440, 2726, 3856, 3966
BshNI GGYRCC 1 cut(s) 4488
BshVI ATCGAT 1 cut(s) 771
BsiEI CGRYCG 1 cut(s) 2727
BsiHKAI GWGCWC 3 cut(s) 30, 256, 4967
BsiHKCI CYCGRG 2 cut(s) 231, 3476
BsiSI CCGG 7 cut(s) 120, 232, 1753, 1824, 2723, 3211, 3963
BslFI GGGAC 7 cut(s) 214, 2057, 2231, 2720, 3379, 3459, 3936
BslI CCNNNNNNNGG 4 cut(s) 85, 1202, 2084, 2270
BsmAI GTCTC 8 cut(s) 1049, 2506, 2537, 2640, 2790, 3125, 3798, 4624
BsmBI CGTCTC 1 cut(s) 2506
BsmFI GGGAC 7 cut(s) 214, 2057, 2231, 2720, 3379, 3459, 3936
BsmI GAATGC 8 cut(s) 175, 833, 2314, 2674, 3147, 3462, 3695, 4025
BsnI GGCC 5 cut(s) 104, 2440, 2726, 3856, 3966
Bso31I GGTCTC 3 cut(s) 2537, 2790, 3798
BsoBI CYCGRG 2 cut(s) 231, 3476
Bsp119I TTCGAA 2 cut(s) 946, 4455
Bsp1286I GDGCHC 5 cut(s) 30, 256, 1107, 4128, 4967
Bsp1407I TGTACA 1 cut(s) 4576
Bsp1720I GCTNAGC 1 cut(s) 414
Bsp19I CCATGG 2 cut(s) 3764, 4692
BspANI GGCC 5 cut(s) 104, 2440, 2726, 3856, 3966
BspDI ATCGAT 1 cut(s) 771
BspFNI CGCG 1 cut(s) 2197
BspHI TCATGA 3 cut(s) 3184, 3744, 4468
BspLI GGNNCC 6 cut(s) 702, 2497, 3073, 4038, 4071, 4490
BspOI GCTAGC 1 cut(s) 1853
BspQI GCTCTTC 3 cut(s) 453, 943, 3569
BspT104I TTCGAA 2 cut(s) 946, 4455
BspT107I GGYRCC 1 cut(s) 4488
BspTNI GGTCTC 3 cut(s) 2537, 2790, 3798
BsrBI CCGCTC 1 cut(s) 1927
BsrDI GCAATG 5 cut(s) 263, 472, 484, 3335, 4422
BsrFI RCCGGY 1 cut(s) 3962
BsrGI TGTACA 1 cut(s) 4576
BsrI ACTGG 6 cut(s) 1277, 1481, 2449, 2762, 4191, 4325
BssAI RCCGGY 1 cut(s) 3962
BssNAI GTATAC 1 cut(s) 2190
BssNI GRCGYC 4 cut(s) 17, 141, 3448, 4592
BssSI CACGAG 2 cut(s) 1317, 1725
BssT1I CCWWGG 9 cut(s) 20, 704, 1468, 3318, 3452, 3641, 3764, 4356, 4692
Bst1107I GTATAC 1 cut(s) 2190
Bst2BI CACGAG 2 cut(s) 1317, 1725
Bst2UI CCWGG 5 cut(s) 101, 969, 2430, 3111, 3956
Bst6I CTCTTC 8 cut(s) 453, 943, 967, 3252, 3569, 4581, 4706, 4895
BstACI GRCGYC 4 cut(s) 17, 141, 3448, 4592
BstAUI TGTACA 1 cut(s) 4576
BstBI TTCGAA 2 cut(s) 946, 4455
BstC8I GCNNGC 9 cut(s) 125, 739, 1161, 1341, 1851, 2383, 3652, 3964, 4281
BstDSI CCRYGG 3 cut(s) 2195, 3764, 4692
BstEII GGTNACC 1 cut(s) 1000
BstFNI CGCG 1 cut(s) 2197
BstH2I RGCGCY 2 cut(s) 3359, 4655
BstHHI GCGC 3 cut(s) 1159, 3358, 4654
BstMAI GTCTC 8 cut(s) 1049, 2506, 2537, 2640, 2790, 3125, 3798, 4624
BstMCI CGRYCG 1 cut(s) 2727
BstNI CCWGG 5 cut(s) 101, 969, 2430, 3111, 3956
BstNSI RCATGY 5 cut(s) 844, 1821, 2231, 2610, 4283
BstPI GGTNACC 1 cut(s) 1000
BstSCI CCNGG 9 cut(s) 99, 230, 231, 967, 1752, 1823, 2428, 3109, 3954
BstSFI CTRYAG 3 cut(s) 246, 2779, 2790
BstSLI GKGCMC 1 cut(s) 1107
BstUI CGCG 1 cut(s) 2197
BstV1I GCAGC 8 cut(s) 1680, 2218, 2221, 2654, 2895, 3250, 3386, 4669
BstV2I GAAGAC 9 cut(s) 53, 183, 935, 1272, 1769, 2771, 3092, 3161, 4455
BstX2I RGATCY 7 cut(s) 91, 700, 2495, 2795, 4062, 4382, 4520
BstXI CCANNNNNNTGG 3 cut(s) 268, 997, 4699
BstYI RGATCY 7 cut(s) 91, 700, 2495, 2795, 4062, 4382, 4520
BstZ17I GTATAC 1 cut(s) 2190
BstZI CGGCCG 1 cut(s) 2724
Bsu15I ATCGAT 1 cut(s) 771
Bsu36I CCTNAGG 1 cut(s) 2997
BsuI GTATCC 2 cut(s) 1825, 3117
BsuRI GGCC 5 cut(s) 104, 2440, 2726, 3856, 3966
BsuTUI ATCGAT 1 cut(s) 771
BtgI CCRYGG 3 cut(s) 2195, 3764, 4692
BtsI GCAGTG 3 cut(s) 328, 408, 2736
Cac8I GCNNGC 9 cut(s) 125, 739, 1161, 1341, 1851, 2383, 3652, 3964, 4281
CaiI CAGNNNCTG 1 cut(s) 4046
CciI TCATGA 3 cut(s) 3184, 3744, 4468
CfoI GCGC 3 cut(s) 1159, 3358, 4654
Cfr10I RCCGGY 1 cut(s) 3962
Cfr13I GGNCC 2 cut(s) 333, 3854
Cfr42I CCGCGG 1 cut(s) 2198
Cfr9I CCCGGG 1 cut(s) 231
ClaI ATCGAT 1 cut(s) 771
CseI GACGC 3 cut(s) 25, 782, 4581
CspCI CAANNNNNGTGG 2 cut(s) 1096, 1131
EaeI YGGCCR 2 cut(s) 2724, 3964
EagI CGGCCG 1 cut(s) 2724
Eam1104I CTCTTC 8 cut(s) 453, 943, 967, 3252, 3569, 4581, 4706, 4895
EarI CTCTTC 8 cut(s) 453, 943, 967, 3252, 3569, 4581, 4706, 4895
EciI GGCGGA 1 cut(s) 1043
EclXI CGGCCG 1 cut(s) 2724
Eco130I CCWWGG 9 cut(s) 20, 704, 1468, 3318, 3452, 3641, 3764, 4356, 4692
Eco24I GRGCYC 1 cut(s) 4128
Eco31I GGTCTC 3 cut(s) 2537, 2790, 3798
Eco32I GATATC 1 cut(s) 4941
Eco47I GGWCC 1 cut(s) 333
Eco47III AGCGCT 2 cut(s) 3357, 4653
Eco52I CGGCCG 1 cut(s) 2724
Eco81I CCTNAGG 1 cut(s) 2997
Eco88I CYCGRG 2 cut(s) 231, 3476
Eco91I GGTNACC 1 cut(s) 1000
EcoO65I GGTNACC 1 cut(s) 1000
EcoRI GAATTC 6 cut(s) 872, 2164, 3188, 3872, 4324, 5050
EcoRII CCWGG 5 cut(s) 99, 967, 2428, 3109, 3954
EcoRV GATATC 1 cut(s) 4941
EcoT14I CCWWGG 9 cut(s) 20, 704, 1468, 3318, 3452, 3641, 3764, 4356, 4692
EcoT22I ATGCAT 5 cut(s) 982, 1511, 3064, 3301, 3695
EcoT38I GRGCYC 1 cut(s) 4128
ErhI CCWWGG 9 cut(s) 20, 704, 1468, 3318, 3452, 3641, 3764, 4356, 4692
Esp3I CGTCTC 1 cut(s) 2506
FaqI GGGAC 7 cut(s) 214, 2057, 2231, 2720, 3379, 3459, 3936
FauI CCCGC 1 cut(s) 2092
FbaI TGATCA 7 cut(s) 405, 912, 1855, 2857, 3181, 4969, 4995
FblI GTMKAC 1 cut(s) 2189
FriOI GRGCYC 1 cut(s) 4128
FspBI CTAG 7 cut(s) 1850, 1989, 2027, 2661, 3642, 5015, 5104
GlaI GCGC 3 cut(s) 1158, 3357, 4653
GsuI CTGGAG 3 cut(s) 240, 4174, 4911
HaeII RGCGCY 2 cut(s) 3359, 4655
HaeIII GGCC 5 cut(s) 104, 2440, 2726, 3856, 3966
HapII CCGG 7 cut(s) 120, 232, 1753, 1824, 2723, 3211, 3963
HgaI GACGC 3 cut(s) 25, 782, 4581
HhaI GCGC 3 cut(s) 1159, 3358, 4654
Hin1I GRCGYC 4 cut(s) 17, 141, 3448, 4592
Hin6I GCGC 3 cut(s) 1157, 3356, 4652
HinP1I GCGC 3 cut(s) 1157, 3356, 4652
HincII GTYRAC 2 cut(s) 2339, 2509
HindII GTYRAC 2 cut(s) 2339, 2509
HindIII AAGCTT 4 cut(s) 1168, 1394, 1931, 2204
HpaII CCGG 7 cut(s) 120, 232, 1753, 1824, 2723, 3211, 3963
Hpy99I CGWCG 2 cut(s) 13, 3596
HpyCH4IV ACGT 4 cut(s) 141, 227, 940, 3448
HpySE526I ACGT 4 cut(s) 141, 227, 940, 3448
Hsp92I GRCGYC 4 cut(s) 17, 141, 3448, 4592
HspAI GCGC 3 cut(s) 1157, 3356, 4652
KpnI GGTACC 1 cut(s) 4492
KroI GCCGGC 1 cut(s) 3962
KroNI GCCGGC 1 cut(s) 3964
Ksp22I TGATCA 7 cut(s) 405, 912, 1855, 2857, 3181, 4969, 4995
KspI CCGCGG 1 cut(s) 2198
LguI GCTCTTC 3 cut(s) 453, 943, 3569
Lsp1109I GCAGC 8 cut(s) 1680, 2218, 2221, 2654, 2895, 3250, 3386, 4669
MaeI CTAG 7 cut(s) 1850, 1989, 2027, 2661, 3642, 5015, 5104
MaeII ACGT 4 cut(s) 141, 227, 940, 3448
MbiI CCGCTC 1 cut(s) 1927
MfeI CAATTG 4 cut(s) 726, 1585, 2577, 4139
MflI RGATCY 7 cut(s) 91, 700, 2495, 2795, 4062, 4382, 4520
MhlI GDGCHC 5 cut(s) 30, 256, 1107, 4128, 4967
MlyI GAGTC 7 cut(s) 305, 1247, 1765, 3361, 3769, 3812, 4481
MmeI TCCRAC 1 cut(s) 307
Mph1103I ATGCAT 5 cut(s) 982, 1511, 3064, 3301, 3695
MroNI GCCGGC 1 cut(s) 3962
MroXI GAANNNNTTC 5 cut(s) 162, 942, 2312, 2627, 4725
MslI CAYNNNNRTG 6 cut(s) 477, 995, 1613, 3011, 4030, 4697
MspA1I CMGCKG 3 cut(s) 803, 2197, 4763
MspI CCGG 7 cut(s) 120, 232, 1753, 1824, 2723, 3211, 3963
MspR9I CCNGG 9 cut(s) 101, 232, 233, 969, 1754, 1825, 2430, 3111, 3956
MunI CAATTG 4 cut(s) 726, 1585, 2577, 4139
Mva1269I GAATGC 8 cut(s) 175, 833, 2314, 2674, 3147, 3462, 3695, 4025
MvaI CCWGG 5 cut(s) 101, 969, 2430, 3111, 3956
MvnI CGCG 1 cut(s) 2197
NaeI GCCGGC 1 cut(s) 3964
NciI CCSGG 4 cut(s) 232, 233, 1754, 1825
NcoI CCATGG 2 cut(s) 3764, 4692
NgoMIV GCCGGC 1 cut(s) 3962
NheI GCTAGC 1 cut(s) 1849
NlaIV GGNNCC 6 cut(s) 702, 2497, 3073, 4038, 4071, 4490
NmeAIII GCCGAG 2 cut(s) 3982, 4653
NmuCI GTSAC 5 cut(s) 1000, 1604, 1768, 2609, 4593
NsiI ATGCAT 5 cut(s) 982, 1511, 3064, 3301, 3695
NspI RCATGY 5 cut(s) 844, 1821, 2231, 2610, 4283
NspV TTCGAA 2 cut(s) 946, 4455
PaeI GCATGC 1 cut(s) 4283
PagI TCATGA 3 cut(s) 3184, 3744, 4468
PciI ACATGT 1 cut(s) 840
PciSI GCTCTTC 3 cut(s) 453, 943, 3569
PcsI WCGNNNNNNNCGW 2 cut(s) 138, 2046
PctI GAATGC 8 cut(s) 175, 833, 2314, 2674, 3147, 3462, 3695, 4025
PdiI GCCGGC 1 cut(s) 3964
PdmI GAANNNNTTC 5 cut(s) 162, 942, 2312, 2627, 4725
PflFI GACNNNGTC 2 cut(s) 602, 2048
PflMI CCANNNNNTGG 1 cut(s) 2270
PfoI TCCNGGA 1 cut(s) 1823
PleI GAGTC 7 cut(s) 305, 1246, 1765, 3361, 3769, 3811, 4480
PpsI GAGTC 7 cut(s) 305, 1246, 1765, 3361, 3769, 3811, 4480
PscI ACATGT 1 cut(s) 840
PshBI ATTAAT 1 cut(s) 1334
PsiI TTATAA 2 cut(s) 2349, 2951
Psp6I CCWGG 5 cut(s) 99, 967, 2428, 3109, 3954
PspEI GGTNACC 1 cut(s) 1000
PspGI CCWGG 5 cut(s) 99, 967, 2428, 3109, 3954
PspN4I GGNNCC 6 cut(s) 702, 2497, 3073, 4038, 4071, 4490
PspPI GGNCC 2 cut(s) 333, 3854
PstNI CAGNNNCTG 1 cut(s) 4046
PsuI RGATCY 7 cut(s) 91, 700, 2495, 2795, 4062, 4382, 4520
PsyI GACNNNGTC 2 cut(s) 602, 2048
PvuII CAGCTG 2 cut(s) 803, 4763
RseI CAYNNNNRTG 6 cut(s) 477, 995, 1613, 3011, 4030, 4697
SacII CCGCGG 1 cut(s) 2198
SapI GCTCTTC 3 cut(s) 453, 943, 3569
Sau96I GGNCC 2 cut(s) 333, 3854
ScaI AGTACT 1 cut(s) 188
SchI GAGTC 7 cut(s) 305, 1247, 1765, 3361, 3769, 3812, 4481
ScrFI CCNGG 9 cut(s) 101, 232, 233, 969, 1754, 1825, 2430, 3111, 3956
SduI GDGCHC 5 cut(s) 30, 256, 1107, 4128, 4967
SfcI CTRYAG 3 cut(s) 246, 2779, 2790
Sfr303I CCGCGG 1 cut(s) 2198
SfuI TTCGAA 2 cut(s) 946, 4455
SgrBI CCGCGG 1 cut(s) 2198
SinI GGWCC 1 cut(s) 333
SmaI CCCGGG 1 cut(s) 233
SmiMI CAYNNNNRTG 6 cut(s) 477, 995, 1613, 3011, 4030, 4697
SmlI CTYRAG 6 cut(s) 212, 1094, 2210, 2903, 4018, 5093
SmoI CTYRAG 6 cut(s) 212, 1094, 2210, 2903, 4018, 5093
SpeI ACTAGT 1 cut(s) 2026
SphI GCATGC 1 cut(s) 4283
SspI AATATT 1 cut(s) 1721
SspMI CTAG 7 cut(s) 1850, 1989, 2027, 2661, 3642, 5015, 5104
StyD4I CCNGG 9 cut(s) 99, 230, 231, 967, 1752, 1823, 2428, 3109, 3954
StyI CCWWGG 9 cut(s) 20, 704, 1468, 3318, 3452, 3641, 3764, 4356, 4692
TaiI ACGT 4 cut(s) 144, 230, 943, 3451
TaqI TCGA 9 cut(s) 606, 771, 946, 1712, 3280, 3594, 4313, 4455, 4543
TatI WGTACW 4 cut(s) 186, 1554, 3558, 4576
TauI GCSGC 2 cut(s) 8, 676
TseFI GTSAC 5 cut(s) 1000, 1604, 1768, 2609, 4593
TseI GCWGC 8 cut(s) 1668, 2231, 2234, 2642, 2908, 3263, 3399, 4682
Tsp45I GTSAC 5 cut(s) 1000, 1604, 1768, 2609, 4593
TspGWI ACGGA 4 cut(s) 58, 101, 2488, 2761
TspMI CCCGGG 1 cut(s) 231
Tth111I GACNNNGTC 2 cut(s) 602, 2048
Van91I CCANNNNNTGG 1 cut(s) 2270
VpaK11BI GGWCC 1 cut(s) 333
VspI ATTAAT 1 cut(s) 1334
XceI RCATGY 5 cut(s) 844, 1821, 2231, 2610, 4283
XcmI CCANNNNNNNNNTGG 1 cut(s) 4080
XmaI CCCGGG 1 cut(s) 231
XmaJI CCTAGG 1 cut(s) 3641
XmiI GTMKAC 1 cut(s) 2189
XmnI GAANNNNTTC 5 cut(s) 162, 942, 2312, 2627, 4725
XspI CTAG 7 cut(s) 1850, 1989, 2027, 2661, 3642, 5015, 5104
ZraI GACGTC 2 cut(s) 142, 3449
ZrmI AGTACT 1 cut(s) 188
Zsp2I ATGCAT 5 cut(s) 982, 1511, 3064, 3301, 3695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.