Rh7AG101400

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000526
Physical Location & Seq
Forward (+)
7835380 .. 7840013
4634 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG101400.1

Sequence Viewer

Length: 4269 bp
ATGTGGAAACAAAAGTTTCCTGTCAGACAGGAATTCAGAGTTGAGAGGAGAATGGAGGTGGATGAGTGGGTGATCACTCTTGCATTTCCAAATGGAGAACGTCTTCGAAGAGCAAGGAACTCATCGCCTGGTGTTTATGCATTTCTTCCCACAGAGATGGTCACCAACTTTCCCTTCATAATACAGGCTGATTTTCTTTTAGCATCTTCAAGAGAGACAATCCTACTTGACAACAAGTGGAATCAAGGGATTCTTGAGTGTGTGCCCACCGCATTTTCACATGCTTTTAACTCTCTTGTGAAAACAGTGGAAGGTGCGCCAACATCAAGTTTGCCTCCTATGTTCAGATTCTTGCCTGTTCAGGTTTCTTCCTATAAAGAATTAAATGCTGTAAGGGAGTCAATAAAAGCAAAGATGGTTGACGAAGACATTATACCAGTTGAGCCTGAAATGGAGCAGAGGTTCTTCCACAAGCCTCGTGAAGTGGGAAGATTAATGCCTGCCTTTTGGACTATTTTGAGGAAGGCGAGGGTTCAGGGTGTAAGCTTGATTAAGCTTTCATCACATGGAAGATTTGTTCTGTCTTATTCCTTTGACAGCAAGGAATATGATCCTATACTAAGTTTCCTTGGAGTTGAACTAGTGAATGATGCCTGGTATGTTAAATGCATACAGGGTACTTCTGATGTTGTTGATCGAGTGTCAGAAGATGTGTACTTAGAGCTTCTACTGTTTATCGCTGACAATTGGGCTTCTAAATTTGGTCACACCAACATGAAGAACATACCATTGATAAAATATGCAGACTGTAGAAATGAATCTTTATGCAGCATAAGCGAAATCCAGAAAGGTGAATGTAGAGTTTTCTTATCGAGCAAAAGTTCTCATGTTTCATGGCTGATTGATTGCAACCGGGAATTTAATTCTGTGACTCGTCTTCTCTTTATGCCAAAAGCCACTCAGGAAGCTATCTGGGCATGCTCTGGGAAGGATACATTGAAGAAATGGCTAGCTGATCAAGCTAAGGTTGGTTCTGTTGATGTCAATAAATATGCAGTTGATCTACTTCAGAATTCTCTTCATGAGCGGAAGCTTGTTATTGCATATGTTCATTTCTTATATCACTCGGTTGATAATAATTTTATCTCAAGTCGGGAAGTTGACCGTTTATGTGGAATTATGCCACTAGTGGATGGCTATGGGAACATCATCAGAAGTAGAAAAGGCGTTGTTGTTGTTCCTGCCAATGGAAGCAAATGGGCGGGATTGACTGATTCTAATTTGTGGAGAAAAGAAGGCCATGTTGAATTAGGGGAGCATTATATGTATTCTGGCTACTTTTCTGGTAAGTATACACCGGAGAAGAAGCTTCTTGAGTTCCTTATAAAACATGTTGCTGCTTTGGATGTCCCTCATATTTCTGCCCCAAGTGATGGTATCACGTCTGTATCTGCACCACTAACTAAGCAGAATGCCTTCTTGCTTTTGGATTGGATTCGTCAACTGAATTATAAAAGGGTTGGCATCCCTCACAAATTCTTGGCTTGCATAAACGAAGGTAGTTGGCTGAAAGTTACTTTGAATGGCTCTCCTGGTTATAGGCCGCCATCCCAGTCATTTCTGCTCAACTCATCATGGGGAAATACTTTGCAGAATGGATCCGTCTCTGTTGACATTCCATTGATTGATACGAGCTTTTATGGTGAGAGAATTAGTGGATACAAGGAGGAGCTAAAGACAATTGGGGTCATGTTTGAATATAGCGAAGCATGTGACTTCATTGGGAAGCATTTCATGTCTCTGGCAGCTTCATCCTCTTTAACTAGAGGAAATGTGCATTCCATTCTGCGTTTCATCAGGTTTTTAAGGGACAAACTTCTTTCTCCGGCCGATTTTATCTCTGCTATTAAGAAAGGGAACTGGCTGAAGACTTCCGTTGGCTACAGGTCTCCTGTAGGATCTGTTTTGTCAGATAAGGAGTGGAATGTTGCCTCCAAAATTAGCAACATTCCCTTCATTGATCAGGACTTTTACGGAGATGAAATCTGTAATTTCAGAACTGAACTTGGGCTGCTTGGTGTTATAATCTCCTTCAACAGAAGTTACCAACTTATAATTGACAACCTGAAGCCACCATCTTGCATAACTTCTTGGACACCTGAGGTTGTTACCTTAATGCTCAACTGTATGGCTCTATCCAAATCATCTGACAAACTTGTCAATGCACTGAAAGGAGTCAAGTGCCTGAAGACAATTATTGGTTACAAGTGTCCAGGGGAATGTCTCTTATTTCATCACGAATGGGGTTGCATTCTTCAGGTCGTCAATGGTCTTCCCGTGATTGATCATGAATTCTATGGGAGCAACATTTTCCGGTACATAAATGAACTGAAAAAAATTGGTGTGGTGGTTGATTTTGAAGGGGCTGCAAAAGTACTTGCTCGATATTTCAGGCACTATGCAAAATCAGCTTCCATCACCAAGGAAAATGTTGCATCATTGCTTCTGTGTTACAGAGAGCTGAATGGGACTCCATATAAATTTCCTGCTGATATTAGGCGCTGCTTCGCTAAGGTCAAGTGGTTGCGTAGTCGTTTTGGTGATTACAGATGTCCAAGGGAGTGCATTCTCTTTAGTCCCGAGTGGGAATCTATATCCCCAATAACTCTGCTTCCTTTCATTGATGATTCTGACAGTTACTATGGAAAGGGCATACATGAGTACAGAAAAGAGTTGAAGAGCTTGGGGGTTGTCGTCGAATACACGGAAGGAGTTAAGTTTGTGGCTCGTGGTCTTTATCTCCCTAGGGATGCCAGCAGTATTTTTCCTGTAAATGCACTTGCGTTGCTGGAATGCATACGTGTTTTCCAGAAGAAAGGTTACCCCTTTCTTGATAATTCTGATTTCATGAAGAAAGTTTCTCAACCATGGATAAAGACTCATGCTGGTTATTGGTCTCCCAAAGAGTCTTTGTTCTTTGATTCCAAGTTTGGTTTATATTTGAAGCGGACTGATGGGCCTTTCATTGATGAAGAATTCTATGGTACTAAGATTGCATCTTACAAAAAAGAGCTGTCTGCATTGGGGGTTATTGTTGAAGCTGAAAAGGGATGTCCCTTGATTGCCGGCCACCTTGATATTCATGATGAGACTGAAGCTTTTGTTCGGTTGTATAATTACTTGAGTGAGTTCCAGTGGGAGCCAGACACTGACGCTGCTCGAAGGATCTGGATTCCAAAAGGGGAGTGGGTTCGTGCTGATGAATGTGTTATTTATGACAAGGATGGGCTATTTGGTTTGCAGTTGACTGTTTTGGAGAAACATTATGAGCATAAACTTCTGCTTTTCTTCTCCAGTGCGTTTGGAGTTAAATCTCATCCTTCAGTTGATGATTACTTGAAGGTTTGGAAGGTCTGGGAAAGTTCTGGAAGTGCATTGTCGCATGCTGATTGTTGCAAGTTTTGGAGTTATATCTCGAAGCACTGGAATTCAAAGACAGAAAAGACTGTTGCTGATGCCTTGGTGAAAGTACCTGTCAGTTCTGGATCTGAAGGAATCTTGCTGTGCTACAAGTCCGATGTTTTCATTGCTGATGACCTTCATCTGAAGTATCTATTTGAACAGTCTTCTCCCGAGTCTATTTTTGTTTGGTACCCTCAACCAAGTTTGCCTTCCATTCCAAGATCCAAGTTGCTTGACATTTATCGAAGAATTGGTGTTCGCACTATATCTGAATCTGTACAAAAGGAAGAGGCGTCACTTGCAGATGATGTTGAAGCAGTCTTTTTGCCGAGGGAAAAGCTGATTAAGAAAGCGCTGTTCAGGCTGATTCTTGGTTTTCTTGCTGCTCCTCCCATGGAAATGGAAGCTGAGAAGAGGCTTGAAGCTGTTCAATTCCTTGTGAATGTTACTGTGGTGGTTACAGCTGAACCAATCTCTGTAAGCTATAGTCTACCTTTATCTTCTTGGAAAGTTTTGAATGTGAGAGGAAGCCAAAAGATACGTTTTGATAGAGACAATTCAAAGATCTTCACTCAGAAAATGGACAAGTCTGGAGGGCAGAAGAGTATGTTTGAGTTTGCTACTTCTTTCTCTCAAGCAATATCTGAGAGTGTGGTATGGGATAGTACGGATCATATAGATTCACTCTCTGAGCTGATCAAGGTGGCTGCTTTGCTAAACTTCAAAGAGGAAGCAGTTGATTCCTTGATGAAGTCAAAGAATTTGCAAATCTTCATGGAGGATGAGGAGTTTCTCAGGACTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1422

Amino Acids

161.83

Weight (kDa)

8.13

Isoelectric Point (pI)

37.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28020 AT3G48770 AT3G48770
fragaria_vesca FvH4_5g09020 FvH4_5g09020
malus_domestica MD06G1092400.v1.1 MD06G1134600.v1.1 MD06G1134700.v1.1 MD06G1134800.v1.1 MD06G1135200.v1.1 MD06G1135400.v1.1 MD06G1135500.v1.1 MD08G1137600.v1.1 MD15G1395100.v1.1
prunus_persica Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145600_v2.0.a1 Prupe.5G145800_v2.0.a1 Prupe.5G145900_v2.0.a1
pyrus_communis pycom06g12550 pycom06g12570 pycom06g12580 pycom06g12590 pycom06g12610 pycom06g12630 pycom06g12640 pycom06g12650 pycom08g06280
rosa_chinensis RchiOBHm_Chr6g0249321 RchiOBHm_Chr6g0275111 RchiOBHm_Chr7g0189191 RchiOBHm_Chr7g0189261
rosa_laevigata RLG00000004622 RLG00000004623 RLG00000008237 RLG00000008238 RLG00000023303 RLG00000035187
rosa_multiflora Rmu_co8294311.1_g000001 Rmu_co8389833.1_g000001 Rmu_sc0001044.1_g000004 Rmu_sc0001329.1_g000011 Rmu_sc0006012.1_g000010 Rmu_sc0007286.1_g000032 Rmu_sc0009360.1_g000016 Rmu_sc0011817.1_g000001 Rmu_sc0015050.1_g000001
rosa_roxburghii Rroxscaffold_1G00064000 Rroxscaffold_3G00265460 Rroxscaffold_3G00265580 Rroxscaffold_3G00265640 Rroxscaffold_3G00265690 Rroxscaffold_3G00265700 Rroxscaffold_7G00193620 Rroxscaffold_7G00204550 Rroxscaffold_7G00217350
rosa_rugosa Rorug01G0465500 Rorug06G0088500 Rorug06G0496700 Rorug06G0496700
rosa_samantha Rh1AG064800 Rh2BG015100 Rh2BG493500 Rh2DG017300 Rh2DG502500 Rh4BG208500 Rh5BG528500 Rh5CG552900 Rh6CG207800 Rh6DG197900 Rh6DG198000 Rh7AG101300 Rh7AG101400 Rh7AG101600 Rh7BG104100 Rh7BG104200 Rh7CG105600 Rh7CG105700 Rh7DG103200 Rh7DG103400
rosa_wichuraiana Rw1G007300 Rw5G034670 Rw7G008750 Rw7G008780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 1385, 1512, 2084, 2114
AasI GACNNNNNNGTC 1 cut(s) 2216
Acc65I GGTACC 1 cut(s) 3651
AccB1I GGYRCC 1 cut(s) 3651
AccB7I CCANNNNNTGG 1 cut(s) 1433
AccBSI CCGCTC 1 cut(s) 1087
AccI GTMKAC 2 cut(s) 1352, 3952
AciI CCGC 5 cut(s) 270, 1087, 1262, 1604, 3007
AclWI GGATC 8 cut(s) 605, 1653, 1666, 1966, 3233, 3553, 3678, 4140
AcoI YGGCCR 2 cut(s) 1887, 3127
AcuI CTGAAG 9 cut(s) 1052, 1946, 2147, 2267, 2300, 3174, 3366, 3570, 3626
AcyI GRCGYC 1 cut(s) 3755
AfeI AGCGCT 1 cut(s) 3816
AfiI CCNNNNNNNGG 2 cut(s) 1247, 1433
AflIII ACRYGT 2 cut(s) 1390, 2860
AhlI ACTAGT 2 cut(s) 640, 1186
AjiI CACGTC 1 cut(s) 1443
AjnI CCWGG 4 cut(s) 127, 653, 1591, 2272
AjuI GAANNNNNNNTTGG 4 cut(s) 158, 190, 3655, 3687
AloI GAACNNNNNNTCC 4 cut(s) 446, 478, 561, 593
Alw26I GTCTC 8 cut(s) 209, 1669, 1803, 1953, 2288, 2961, 3143, 4008
AlwI GGATC 8 cut(s) 605, 1653, 1666, 1966, 3233, 3553, 3678, 4140
AlwNI CAGNNNCTG 1 cut(s) 3209
Ama87I CYCGRG 2 cut(s) 2639, 3632
Aor51HI AGCGCT 1 cut(s) 3816
AoxI GGCC 5 cut(s) 1297, 1601, 1887, 3017, 3127
ApeKI GCWGC 9 cut(s) 828, 1397, 1805, 2071, 2426, 2562, 3215, 3845, 4169
AseI ATTAAT 1 cut(s) 494
Asp700I GAANNNNTTC 4 cut(s) 102, 1475, 1790, 3888
Asp718I GGTACC 1 cut(s) 3651
AspA2I CCTAGG 1 cut(s) 2804
AspLEI GCGC 3 cut(s) 319, 2562, 3817
AspS9I GGNCC 1 cut(s) 3017
AsuC2I CCSGG 1 cut(s) 914
AsuHPI GGTGA 7 cut(s) 82, 154, 863, 1715, 2470, 2612, 3535
AsuII TTCGAA 1 cut(s) 106
AsuNHI GCTAGC 1 cut(s) 1009
AvaI CYCGRG 2 cut(s) 2639, 3632
AvrII CCTAGG 1 cut(s) 2804
AxyI CCTNAGG 1 cut(s) 2160
BaeGI GKGCMC 1 cut(s) 267
BaeI ACNNNNGTAYC 2 cut(s) 1431, 1464
BamHI GGATCC 1 cut(s) 1658
BanI GGYRCC 1 cut(s) 3651
BarI GAAGNNNNNNTAC 4 cut(s) 417, 449, 2864, 2896
BauI CACGAG 2 cut(s) 477, 2787
BbsI GAAGAC 7 cut(s) 95, 432, 929, 1934, 2255, 2324, 3618
BbvI GCAGC 9 cut(s) 840, 1384, 1817, 2058, 2413, 2549, 3202, 3832, 4156
BccI CCATC 9 cut(s) 151, 409, 1187, 1427, 1615, 2143, 2483, 3008, 3278
BciT130I CCWGG 4 cut(s) 129, 655, 1593, 2274
BciVI GTATCC 2 cut(s) 985, 1712
BclI TGATCA 5 cut(s) 72, 1015, 2020, 2344, 4158
BcnI CCSGG 1 cut(s) 914
BcoDI GTCTC 8 cut(s) 209, 1669, 1803, 1953, 2288, 2961, 3143, 4008
BcuI ACTAGT 2 cut(s) 640, 1186
BfaI CTAG 6 cut(s) 641, 1010, 1187, 1824, 2805, 4267
BfmI CTRYAG 4 cut(s) 808, 1942, 1953, 3946
BfoI RGCGCY 2 cut(s) 2563, 3818
BfuI GTATCC 2 cut(s) 985, 1712
BglII AGATCT 1 cut(s) 4026
BlnI CCTAGG 1 cut(s) 2804
BmcAI AGTACT 1 cut(s) 2436
Bme1390I CCNGG 5 cut(s) 129, 655, 914, 1593, 2274
BmeT110I CYCGRG 2 cut(s) 2639, 3632
BmgBI CACGTC 1 cut(s) 1443
BmgT120I GGNCC 1 cut(s) 3017
BmiI GGNNCC 3 cut(s) 1660, 3201, 3653
BmrFI CCNGG 5 cut(s) 129, 655, 914, 1593, 2274
BmrI ACTGGG 1 cut(s) 1606
BmsI GCATC 7 cut(s) 212, 640, 1533, 2504, 2800, 3065, 3505
BmtI GCTAGC 1 cut(s) 1013
BmuI ACTGGG 1 cut(s) 1606
BpiI GAAGAC 7 cut(s) 95, 432, 929, 1934, 2255, 2324, 3618
BpmI CTGGAG 2 cut(s) 3337, 4074
Bpu10I CCTNAGC 2 cut(s) 1023, 2571
Bpu14I TTCGAA 1 cut(s) 106
BpuEI CTTGAG 5 cut(s) 275, 1132, 1394, 3202, 4080
BpuMI CCSGG 1 cut(s) 914
BsaAI YACGTR 1 cut(s) 2861
BsaHI GRCGYC 1 cut(s) 3755
BsaI GGTCTC 2 cut(s) 1953, 2961
BsaJI CCNNGG 9 cut(s) 628, 2273, 2481, 2615, 2804, 2927, 3519, 3791, 3855
BsaWI WCCGGW 2 cut(s) 1357, 2373
BsaXI ACNNNNNCTCC 2 cut(s) 446, 476
Bsc4I CCNNNNNNNGG 2 cut(s) 1247, 1433
Bse118I RCCGGY 1 cut(s) 3125
Bse1I ACTGG 6 cut(s) 437, 1612, 1925, 3193, 3354, 3488
Bse21I CCTNAGG 1 cut(s) 2160
Bse3DI GCAATG 2 cut(s) 2498, 3585
BseBI CCWGG 4 cut(s) 129, 655, 1593, 2274
BseDI CCNNGG 9 cut(s) 628, 2273, 2481, 2615, 2804, 2927, 3519, 3791, 3855
BseLI CCNNNNNNNGG 2 cut(s) 1247, 1433
BseMI GCAATG 2 cut(s) 2498, 3585
BseMII CTCAG 6 cut(s) 974, 2151, 3861, 4049, 4098, 4143
BseNI ACTGG 6 cut(s) 437, 1612, 1925, 3193, 3354, 3488
BseRI GAGGAG 4 cut(s) 61, 1742, 3840, 4262
BseSI GKGCMC 1 cut(s) 267
BseX3I CGGCCG 1 cut(s) 1887
BseXI GCAGC 9 cut(s) 840, 1384, 1817, 2058, 2413, 2549, 3202, 3832, 4156
BsgI GTGCAG 1 cut(s) 1437
Bsh1285I CGRYCG 1 cut(s) 1890
BshFI GGCC 5 cut(s) 1299, 1603, 1889, 3019, 3129
BshNI GGYRCC 1 cut(s) 3651
BsiEI CGRYCG 1 cut(s) 1890
BsiHKCI CYCGRG 2 cut(s) 2639, 3632
BsiSI CCGG 5 cut(s) 913, 1358, 1886, 2374, 3126
BslFI GGGAC 5 cut(s) 1394, 1883, 2542, 2622, 3099
BslI CCNNNNNNNGG 2 cut(s) 1247, 1433
BsmAI GTCTC 8 cut(s) 209, 1669, 1803, 1953, 2288, 2961, 3143, 4008
BsmBI CGTCTC 1 cut(s) 1669
BsmFI GGGAC 5 cut(s) 1394, 1883, 2542, 2622, 3099
BsmI GAATGC 5 cut(s) 1477, 1837, 2310, 2625, 2858
BsnI GGCC 5 cut(s) 1299, 1603, 1889, 3019, 3129
Bso31I GGTCTC 2 cut(s) 1953, 2961
BsoBI CYCGRG 2 cut(s) 2639, 3632
Bsp119I TTCGAA 1 cut(s) 106
Bsp1286I GDGCHC 1 cut(s) 267
Bsp1407I TGTACA 1 cut(s) 3739
Bsp19I CCATGG 2 cut(s) 2927, 3855
BspACI CCGC 5 cut(s) 270, 1087, 1262, 1604, 3007
BspANI GGCC 5 cut(s) 1299, 1603, 1889, 3019, 3129
BspCNI CTCAG 7 cut(s) 973, 2152, 3862, 4048, 4099, 4144, 4269
BspHI TCATGA 4 cut(s) 1081, 2347, 2907, 3142
BspLI GGNNCC 3 cut(s) 1660, 3201, 3653
BspOI GCTAGC 1 cut(s) 1013
BspPI GGATC 8 cut(s) 605, 1653, 1666, 1966, 3233, 3553, 3678, 4140
BspQI GCTCTTC 2 cut(s) 103, 2732
BspT104I TTCGAA 1 cut(s) 106
BspT107I GGYRCC 1 cut(s) 3651
BspTNI GGTCTC 2 cut(s) 1953, 2961
BsrBI CCGCTC 1 cut(s) 1087
BsrDI GCAATG 2 cut(s) 2498, 3585
BsrFI RCCGGY 1 cut(s) 3125
BsrGI TGTACA 1 cut(s) 3739
BsrI ACTGG 6 cut(s) 437, 1612, 1925, 3193, 3354, 3488
BssAI RCCGGY 1 cut(s) 3125
BssECI CCNNGG 9 cut(s) 628, 2273, 2481, 2615, 2804, 2927, 3519, 3791, 3855
BssNAI GTATAC 1 cut(s) 1353
BssNI GRCGYC 1 cut(s) 3755
BssSI CACGAG 2 cut(s) 477, 2787
BssT1I CCWWGG 7 cut(s) 628, 2481, 2615, 2804, 2927, 3519, 3855
Bst1107I GTATAC 1 cut(s) 1353
Bst2BI CACGAG 2 cut(s) 477, 2787
Bst2UI CCWGG 4 cut(s) 129, 655, 1593, 2274
Bst6I CTCTTC 6 cut(s) 103, 1083, 2732, 3744, 3869, 4058
BstACI GRCGYC 1 cut(s) 3755
BstAUI TGTACA 1 cut(s) 3739
BstBAI YACGTR 1 cut(s) 2861
BstBI TTCGAA 1 cut(s) 106
BstC8I GCNNGC 7 cut(s) 501, 979, 1011, 1546, 2815, 3127, 3444
BstDSI CCRYGG 2 cut(s) 2927, 3855
BstEII GGTNACC 2 cut(s) 160, 2879
BstH2I RGCGCY 2 cut(s) 2563, 3818
BstHHI GCGC 3 cut(s) 319, 2562, 3817
BstMAI GTCTC 8 cut(s) 209, 1669, 1803, 1953, 2288, 2961, 3143, 4008
BstMCI CGRYCG 1 cut(s) 1890
BstMWI GCNNNNNNNGC 6 cut(s) 834, 974, 1019, 2468, 3761, 3823
BstNI CCWGG 4 cut(s) 129, 655, 1593, 2274
BstNSI RCATGY 5 cut(s) 284, 981, 1394, 1773, 3446
BstPI GGTNACC 2 cut(s) 160, 2879
BstSCI CCNGG 5 cut(s) 127, 653, 912, 1591, 2272
BstSFI CTRYAG 4 cut(s) 808, 1942, 1953, 3946
BstSLI GKGCMC 1 cut(s) 267
BstV1I GCAGC 9 cut(s) 840, 1384, 1817, 2058, 2413, 2549, 3202, 3832, 4156
BstV2I GAAGAC 7 cut(s) 95, 432, 929, 1934, 2255, 2324, 3618
BstX2I RGATCY 6 cut(s) 1658, 1958, 3225, 3545, 3683, 4026
BstXI CCANNNNNNTGG 2 cut(s) 157, 3862
BstYI RGATCY 6 cut(s) 1658, 1958, 3225, 3545, 3683, 4026
BstZ17I GTATAC 1 cut(s) 1353
BstZI CGGCCG 1 cut(s) 1887
Bsu36I CCTNAGG 1 cut(s) 2160
BsuI GTATCC 2 cut(s) 985, 1712
BsuRI GGCC 5 cut(s) 1299, 1603, 1889, 3019, 3129
BtgI CCRYGG 2 cut(s) 2927, 3855
BtgZI GCGATG 1 cut(s) 108
BtrI CACGTC 1 cut(s) 1443
BtsIMutI CAGTG 6 cut(s) 312, 2225, 3200, 3207, 3361, 3481
Cac8I GCNNGC 7 cut(s) 501, 979, 1011, 1546, 2815, 3127, 3444
CaiI CAGNNNCTG 1 cut(s) 3209
CciI TCATGA 4 cut(s) 1081, 2347, 2907, 3142
CfoI GCGC 3 cut(s) 319, 2562, 3817
Cfr10I RCCGGY 1 cut(s) 3125
Cfr13I GGNCC 1 cut(s) 3017
CseI GACGC 2 cut(s) 3221, 3744
DrdI GACNNNNNNGTC 1 cut(s) 2216
DseDI GACNNNNNNGTC 1 cut(s) 2216
EaeI YGGCCR 2 cut(s) 1887, 3127
EagI CGGCCG 1 cut(s) 1887
Eam1104I CTCTTC 6 cut(s) 103, 1083, 2732, 3744, 3869, 4058
EarI CTCTTC 6 cut(s) 103, 1083, 2732, 3744, 3869, 4058
EclXI CGGCCG 1 cut(s) 1887
Eco130I CCWWGG 7 cut(s) 628, 2481, 2615, 2804, 2927, 3519, 3855
Eco31I GGTCTC 2 cut(s) 1953, 2961
Eco47III AGCGCT 1 cut(s) 3816
Eco52I CGGCCG 1 cut(s) 1887
Eco57I CTGAAG 9 cut(s) 1052, 1946, 2147, 2267, 2300, 3174, 3366, 3570, 3626
Eco81I CCTNAGG 1 cut(s) 2160
Eco88I CYCGRG 2 cut(s) 2639, 3632
Eco91I GGTNACC 2 cut(s) 160, 2879
EcoO65I GGTNACC 2 cut(s) 160, 2879
EcoRI GAATTC 5 cut(s) 32, 1072, 2351, 3035, 3487
EcoRII CCWGG 4 cut(s) 127, 653, 1591, 2272
EcoT14I CCWWGG 7 cut(s) 628, 2481, 2615, 2804, 2927, 3519, 3855
EcoT22I ATGCAT 3 cut(s) 142, 671, 2858
ErhI CCWWGG 7 cut(s) 628, 2481, 2615, 2804, 2927, 3519, 3855
Esp3I CGTCTC 1 cut(s) 1669
FalI AAGNNNNNCTT 4 cut(s) 237, 269, 3655, 3687
FaqI GGGAC 5 cut(s) 1394, 1883, 2542, 2622, 3099
FauI CCCGC 1 cut(s) 1255
FauNDI CATATG 1 cut(s) 1105
FbaI TGATCA 5 cut(s) 72, 1015, 2020, 2344, 4158
FblI GTMKAC 2 cut(s) 1352, 3952
FspBI CTAG 6 cut(s) 641, 1010, 1187, 1824, 2805, 4267
GlaI GCGC 3 cut(s) 318, 2561, 3816
GsuI CTGGAG 2 cut(s) 3337, 4074
HaeII RGCGCY 2 cut(s) 2563, 3818
HaeIII GGCC 5 cut(s) 1299, 1603, 1889, 3019, 3129
HapII CCGG 5 cut(s) 913, 1358, 1886, 2374, 3126
HgaI GACGC 2 cut(s) 3221, 3744
HhaI GCGC 3 cut(s) 319, 2562, 3817
Hin1I GRCGYC 1 cut(s) 3755
Hin6I GCGC 3 cut(s) 317, 2560, 3815
HinP1I GCGC 3 cut(s) 317, 2560, 3815
HincII GTYRAC 5 cut(s) 421, 1162, 1502, 1672, 3306
HindII GTYRAC 5 cut(s) 421, 1162, 1502, 1672, 3306
HindIII AAGCTT 5 cut(s) 544, 554, 1091, 1367, 3156
HpaII CCGG 5 cut(s) 913, 1358, 1886, 2374, 3126
HphI GGTGA 7 cut(s) 82, 154, 863, 1715, 2470, 2612, 3535
Hpy166II GTNNAC 8 cut(s) 421, 715, 1162, 1353, 1502, 1672, 3306, 3953
Hpy8I GTNNAC 8 cut(s) 421, 715, 1162, 1353, 1502, 1672, 3306, 3953
Hpy99I CGWCG 1 cut(s) 2759
HpyCH4IV ACGT 4 cut(s) 100, 1442, 2860, 4003
HpyF10VI GCNNNNNNNGC 6 cut(s) 834, 974, 1019, 2468, 3761, 3823
HpySE526I ACGT 4 cut(s) 100, 1442, 2860, 4003
Hsp92I GRCGYC 1 cut(s) 3755
HspAI GCGC 3 cut(s) 317, 2560, 3815
KpnI GGTACC 1 cut(s) 3655
KroI GCCGGC 1 cut(s) 3125
KroNI GCCGGC 1 cut(s) 3127
Ksp22I TGATCA 5 cut(s) 72, 1015, 2020, 2344, 4158
LguI GCTCTTC 2 cut(s) 103, 2732
LmnI GCTCC 6 cut(s) 454, 1315, 1729, 2361, 3199, 3853
Lsp1109I GCAGC 9 cut(s) 840, 1384, 1817, 2058, 2413, 2549, 3202, 3832, 4156
LweI GCATC 7 cut(s) 212, 640, 1533, 2504, 2800, 3065, 3505
MaeI CTAG 6 cut(s) 641, 1010, 1187, 1824, 2805, 4267
MaeII ACGT 4 cut(s) 100, 1442, 2860, 4003
MbiI CCGCTC 1 cut(s) 1087
MfeI CAATTG 2 cut(s) 745, 1740
MflI RGATCY 6 cut(s) 1658, 1958, 3225, 3545, 3683, 4026
MhlI GDGCHC 1 cut(s) 267
MlyI GAGTC 7 cut(s) 407, 925, 2244, 2524, 2932, 2975, 3644
Mph1103I ATGCAT 3 cut(s) 142, 671, 2858
MroNI GCCGGC 1 cut(s) 3125
MroXI GAANNNNTTC 4 cut(s) 102, 1475, 1790, 3888
MslI CAYNNNNRTG 3 cut(s) 155, 773, 3860
MspA1I CMGCKG 1 cut(s) 3926
MspI CCGG 5 cut(s) 913, 1358, 1886, 2374, 3126
MspR9I CCNGG 5 cut(s) 129, 655, 914, 1593, 2274
MunI CAATTG 2 cut(s) 745, 1740
Mva1269I GAATGC 5 cut(s) 1477, 1837, 2310, 2625, 2858
MvaI CCWGG 4 cut(s) 129, 655, 1593, 2274
MwoI GCNNNNNNNGC 6 cut(s) 834, 974, 1019, 2468, 3761, 3823
NaeI GCCGGC 1 cut(s) 3127
NciI CCSGG 1 cut(s) 914
NcoI CCATGG 2 cut(s) 2927, 3855
NdeI CATATG 1 cut(s) 1105
NgoMIV GCCGGC 1 cut(s) 3125
NheI GCTAGC 1 cut(s) 1009
NlaIV GGNNCC 3 cut(s) 1660, 3201, 3653
NmeAIII GCCGAG 1 cut(s) 3816
NmuCI GTSAC 5 cut(s) 160, 764, 928, 1772, 3756
NsiI ATGCAT 3 cut(s) 142, 671, 2858
NspI RCATGY 5 cut(s) 284, 981, 1394, 1773, 3446
NspV TTCGAA 1 cut(s) 106
PaeI GCATGC 2 cut(s) 981, 3446
PagI TCATGA 4 cut(s) 1081, 2347, 2907, 3142
PciI ACATGT 1 cut(s) 1390
PciSI GCTCTTC 2 cut(s) 103, 2732
PctI GAATGC 5 cut(s) 1477, 1837, 2310, 2625, 2858
PdiI GCCGGC 1 cut(s) 3127
PdmI GAANNNNTTC 4 cut(s) 102, 1475, 1790, 3888
PflMI CCANNNNNTGG 1 cut(s) 1433
PleI GAGTC 7 cut(s) 406, 925, 2243, 2524, 2932, 2974, 3643
PpsI GAGTC 7 cut(s) 406, 925, 2243, 2524, 2932, 2974, 3643
Ppu21I YACGTR 1 cut(s) 2861
PscI ACATGT 1 cut(s) 1390
PshBI ATTAAT 1 cut(s) 494
PsiI TTATAA 4 cut(s) 1385, 1512, 2084, 2114
Psp6I CCWGG 4 cut(s) 127, 653, 1591, 2272
PspEI GGTNACC 2 cut(s) 160, 2879
PspGI CCWGG 4 cut(s) 127, 653, 1591, 2272
PspN4I GGNNCC 3 cut(s) 1660, 3201, 3653
PspPI GGNCC 1 cut(s) 3017
PstNI CAGNNNCTG 1 cut(s) 3209
PsuI RGATCY 6 cut(s) 1658, 1958, 3225, 3545, 3683, 4026
PvuII CAGCTG 1 cut(s) 3926
RseI CAYNNNNRTG 3 cut(s) 155, 773, 3860
SapI GCTCTTC 2 cut(s) 103, 2732
Sau96I GGNCC 1 cut(s) 3017
ScaI AGTACT 1 cut(s) 2436
SchI GAGTC 7 cut(s) 407, 925, 2244, 2524, 2932, 2975, 3644
ScrFI CCNGG 5 cut(s) 129, 655, 914, 1593, 2274
SduI GDGCHC 1 cut(s) 267
SfaNI GCATC 7 cut(s) 212, 640, 1533, 2504, 2800, 3065, 3505
SfcI CTRYAG 4 cut(s) 808, 1942, 1953, 3946
SfuI TTCGAA 1 cut(s) 106
SmiMI CAYNNNNRTG 3 cut(s) 155, 773, 3860
SmlI CTYRAG 5 cut(s) 254, 1147, 1373, 3181, 4095
SmoI CTYRAG 5 cut(s) 254, 1147, 1373, 3181, 4095
SpeI ACTAGT 2 cut(s) 640, 1186
SphI GCATGC 2 cut(s) 981, 3446
SsiI CCGC 5 cut(s) 270, 1087, 1262, 1604, 3007
SspMI CTAG 6 cut(s) 641, 1010, 1187, 1824, 2805, 4267
StyD4I CCNGG 5 cut(s) 127, 653, 912, 1591, 2272
StyI CCWWGG 7 cut(s) 628, 2481, 2615, 2804, 2927, 3519, 3855
TaiI ACGT 4 cut(s) 103, 1445, 2863, 4006
TaqI TCGA 8 cut(s) 106, 697, 872, 2443, 2757, 3220, 3476, 3706
TatI WGTACW 4 cut(s) 714, 2434, 2721, 3739
TauI GCSGC 1 cut(s) 1606
TscAI CASTG 6 cut(s) 312, 2232, 3200, 3214, 3361, 3488
TseFI GTSAC 5 cut(s) 160, 764, 928, 1772, 3756
TseI GCWGC 9 cut(s) 828, 1397, 1805, 2071, 2426, 2562, 3215, 3845, 4169
Tsp45I GTSAC 5 cut(s) 160, 764, 928, 1772, 3756
TspGWI ACGGA 5 cut(s) 1651, 1924, 2049, 2780, 4145
TspRI CASTG 6 cut(s) 312, 2232, 3200, 3214, 3361, 3488
Van91I CCANNNNNTGG 1 cut(s) 1433
VspI ATTAAT 1 cut(s) 494
XceI RCATGY 5 cut(s) 284, 981, 1394, 1773, 3446
XcmI CCANNNNNNNNNTGG 1 cut(s) 3243
XmaJI CCTAGG 1 cut(s) 2804
XmiI GTMKAC 2 cut(s) 1352, 3952
XmnI GAANNNNTTC 4 cut(s) 102, 1475, 1790, 3888
XspI CTAG 6 cut(s) 641, 1010, 1187, 1824, 2805, 4267
ZrmI AGTACT 1 cut(s) 2436
Zsp2I ATGCAT 3 cut(s) 142, 671, 2858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.