Rh7AG101600

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000526
Physical Location & Seq
Forward (+)
7907983 .. 7914721
6739 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG101600.1

Sequence Viewer

Length: 5100 bp
ATGGCGACGACGATGACGCCAAGGGAACACATAGAAGATATACGGAAGACCAAGTTCTCCATCGGAGAAAAGCTCAACCCTTTAACGGAGGATCTTCACCAGGCCGTCAAGAACCTCTCCGGCGAGCTTTACGCTAAAGACGTCCACTTTTTCATGGAACTCGTTCAGAATGCAGAAGACAATGAGTACTCGGAGGGGGTTGATCCATCCCTTGAGTTTGTAATTACATACCGCGATATAACGGCTACAGGGGCTCCAGCCACATTGCTGGTTTTCAATAATGAAAAGGGGTTTTCTCCCAAAAATATTGACTCCATTTGCAGTGTTGGACGGTCCACCAAGAAAGGCAACAGGAAGCGTGGTTATATTGGGGAGAAAGGAATTGGCTTCAAGAGTGTGTTTCTGATCACTGCTCAGCCTTACATATTTAGTAATGGTTATCAGATAAGGTTCAGCGAAGAACCTTGTTCACATTGCAATGTTGGCTACATCGTACCTGAATGGGTTGAGCAGAATCCCACTCTATCTGATATAGAAAAGATATATGGTTCTGCTACTGCCCTTCCAACCACAACACTGATCTTACCTCTGAAGCCCGACAAGGTTGAACCTGTGAAGAAACAACTCTCGATCATTCATCCAGAAGTTCTCTTGTTCCTATCAAAGATTAAGCGGCTCTCTGTTAGGGAAGACAAAGAGGACCCAAGGCAGAACACTGTGACTGCAATTGAGATAGCACGCGAGACTGAACATGCGTCAAGGAAGAACATTGATGCTCAATCCTACACTCTGCATCTTTCAGCGGAAGAAAATGGTGGAAATGGAGAATGCAGTTACTACATGTGGAAACAAAAGTTTCCTGTCAGGCAGGAATTCAGAGTTGAGAGGAGAATGGAGGTGGATGAGTGGGTGATCACTCTTGCATTTCCAATTGGAGAACGTCTTCAAAGAGCAAGGAACTCTTCGCCTGGTGTTTATGCATTTCTTCCCACAGAAATGGTCACCAACTTTCCCTTCATAATACAGGCGGATTTTCTTTTAGCATCTTCAAGAGAGACAATCCTACTTGACAACAAGTGGAATCAAGGGATTCTTGAGTGTGTGCCCACCGCATTTTCCCATGCTTTTAACTCTCTTGTGAAAACTGTGGAAGGTGCGCCAACATCAAGTTTGCCTCCTATGTTCAGATTCTTGCCTGTTCAGGTTTCTTCCTATAAAGAATTAAATGCTGTAAGGGAGTCAATAAAAGCAAAGATGGTTGAGGAAGAAATTATACCAGTTGAGCCCGAAATGGAGCAAAAGTTCTTCCACAAGCCTCGTGAAGTGGGAAGATTAATGCCTGCCTTTTGGTCTATTTTGAGGAAGGCGAGGGTTCAAGGTGTAAGCTGGATTAAGCTTTCATCACATGGAAGATTTGTTCTGTCTTATTCCTTTGACAGCGAGGAATATGATCCTATACTAAGTTTCCTTGGAGTAGAACCAGTGAATGATGATTGGTATGTTAAATGCATACAGGGTACTTCTAATCTTGTTGATGGAGTGTTAGAAGATGTGTACTTAGAGCTTCTACTCTTTATCGCTCACAATTGGGGTTTGAAATTTGGTCACACCAACATGAAGAACATACCATTGATAAAATATGCAGACTCTGGAAATGAATCTTTGTGCAGCATAAGCCAAATCCAGAAAGGGGAATGTAGAGTTTGCTTATCGAGCAAATATCCTCGTGTTTCATGGCTGATTGATTGCAACCGGGAATTTAATTCTGTGACTCGTCTTCTCTTTATGCCAAAAGCCACTCAGGAAGCCATCTGGACATGCTCTGGGAAGGATACATTGAAGAAATGGCTAGCTGATCAAGCTAAGGTTGTAGATGTTGATGTCAATCAATATGCAGTTGATCTCCTTCAGAATTCTCTTAATGAGCGGAAGCTTGTTATTGCATATGTTCATTTCTTATATCACTCGGTTGATAATGATTTTATCTCAAGTCGGGAAGTTCACCGTTTATGTGGAATTATGCCACTAGTGGATGGCTATGGGGACATCATCAGGGGTAGAAAAGGGGTTATTGTTCCTGCCAATGGAAGCAAATGGGCGGGATTGACTGATTCTAATTTGTGGAGAAAAGAAGGCTATGTTGAATTAGGAGAGCATTATATGAATTCTGGCTATTTTGCTGGTAAGTATACACTGGAGAAGAAGCTTCTTGAGTTCCTTATAGAACATGCTGCTGCTTTGGATGTCCCTCATATTTCTGCTCCAAGTGATCGTATCTCGTCTGTATCTGCACCACTAACTAAGCAGAATGCCTTTTTGCTTTTGGATTGGATTCGTCAACTGAATTATAAAAGGGTTGTCATCCCTCAGAAGTTCTTGGCTTGCATAAAGGAAGGTAGTTGGCTGAAAGTTACTTTGAATGGCTCTCCTGGTTATAGGCCACCATCCCAGTCATTTCTGCTTAACTCATCATGGGGAAATACTTTGCAGAATGGATCCGTCTCTGTTGACATTCCATTGATTGATACGAGCTTTTATGGTGAGTGCATTAGTGGATACAAGGAGGAGCTAAAGACAATTGGGGTCATGTTTGAATATTGCGAAGCATGTGACTTCATTGGGAAGCATTTCATGTCTCTGGCAGCTTCATCCTCTTTAACTAGAGGAAATGTGCATTCCATTCTGTGTTTCATCAGGTTTTTAAGGGACAAACTTCTTCCTCCGGCCGATTTTATCTCTGCTATTAAGAAAGGGAACTGGCTGAAGACTGCCGTTGGTTACAGGTCTCCTGTAGGATCTGTTTTGTCAGATAAGGAGTGGAATGTTGCCTCCAAAATTAGCAACATTCCCTTCATTGATCAGGACTTTTATGGTGATGAAATCTGTAATTTCAGAACTGAACTTGAGTTGCTCGGTGTTGTAATCTCCTTCAACCACCAACTTATAATTGACAACCTGAAGTCACCATCTTCCATAACTTATTGGACACCTCAGGTTGTCATCTTAATGCTCAACTGTATGGCTCTATCCAAGTCATCTGATAAACTTGTCAATGCATTGAAAGGAGCCAAGTGCCTGAAGACAAGTATTGGTTACAAGTGTCCAGGGGAATGTCTCTTGTTTCATCACGAATGGGGTTGCATTCTTCAGGTCGTCGATGGTCTTCCCTTGATTGATCATGAATTCTATGGGAGCAACATTTTCCTGTACATAAATGAACTGAAAAAAATTGGTGTGGTGGTTGATTTTGAAGGGGCTGCAAAAGTATTTGCTCGATATTTCAGGCACTATGCATCATCAGCTTCCATCACCAAGGAAAATGTTGCATCATTGCTTCTGTGTTACAGAGCGCTGAAGGGGACTCCATATAAATTTCCTACTGATCTTAGGAGCTGCATTGGTGAGGTCAAGTGGTTGCGTAGTCGTTTTGGTGATTTCAGATGTCCAAGGGAGTGCATTCTCTTTAGTACCGAGTGGGAATCTATATCCCCAATAACTATGCTTCCTTTCATTGATGATTCTGACAGTTACTATGGAAAGAGCATACATGAGTACAGAAAAGAGCTGAAGAGCTTGGGGGTTGTCGTCGAATACAAGGAAGGAGTTAAGTTTGTGGCTTCTGGTCTTTATCTCCCTAGGGATGCCAGCCGTATTTTTCCTGTAAATGCACTTGCGTTGCTGGAATACATACGAGTTTTCCAGGAGAAAGGTTACGCCTTTCTTGATAATTCTGATTTCATGAAGAAAGTTTCTCAACCATGGATAAAGACTCATGCTGGTTATAGGTCTCCCAAAGAGTCTTTGTTGTTTGATTCCAAGTTTGGCTTATATTTGAAGCGGACTGATGGGCCTTTCATTGATGAAGAATTCTATGGTACTAAGATTGCATCTTACAAAAAAGAGCTGTCTGCATTGGGGGTTATTGTTGAATTTGAGAAAGGATGTCCCCTGATTGCCAGCCACCTTGATGTTCATTATGAGATTGAAGCATTTGTTCGGCTGTATAATTACTTGAGTGCATTCAAGTGGGAACCAGACACTGACGCTGCTCGAAGGATCTGGATTCCAAAAGGAGAGTGGGTTCGTCCTGATGATTGTGTTATATATGACAAGGATGGGCTCTTTGGTTTACAATTGACTGTTTTGGAGAAACATTATGAACAGAAACTTCTATTTTTCTTCTCCAGTGCGTTTAGAGTTAAATCTCATCCTTCAGTTGATGATTACTTGAAGGTTTGGAAGGTTTGGGAAAGTTCTGGAAGTGCATTGTCGCATGCTGATTGTTGCAAGTTTTGGAGTTATATCTCGAAGCACTGGAATTCAAAGACAGAAAAGACTGTTGCTGATGCCTTGGTGAAAGTACCTGTCAGTTCTGGATCTGAAGGAATCTTGCTGTGCAACAAGTCTGATGTTTTCATTGCTGATGACCTTCAACTGAAGTATCTATTTGAACAGTCTTCTCCTGTGTCTATTTTTGTTTGGTACCCTCAGCCAAGTTTGCCTTCCATTCCAAGATCCAAGTTGCTTGACATTTATCGAAGAATTGGTGTTCGCACTATATCTGAATCTGTACAGAAGGAAGAGGCGTCACTTGCAGATGATGTTGAAGTAGTCTCTTTGCCGAGGGAAAAGCTGATTAAGAAAGCGCTGTTCAGGCTGATTCTTGGTTTTCTTGCTGCTCCTCCCATGGAAATGGAAGCTGAGAAGAGGCTTGAAGTTGTTCAATTCCTTGTGAATGTTACTGTGGTGGTAACAGCTGAACCAATCTCTGTAAGCTATAATCTACCTTTATCTTCTGGGAAAGTTTTGAATGTGAGAGGAAGCCGAAAAATACGGTTTGATAGAGAAAATTCAAAGATCTTCACTCAGAAAATGGACAAGTCTGGAGGGCAGAAGAGTATCATTGAGTTTGCTACTTTTTTCTCCGAAGCGATATCTGAGAGTGTGTTATGGGAGAGCGCTGATCATATAGATTCACTCTCTGAGCTGATCAAGATGGCTTATTTGCTAGACTTCAATGAGGAAGCAGTTGATTTCTTGATGAAGTCCAAGAATTTGCAAACTTTTATGGAGGATGAGGAGTTTCTCAAGACTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1699

Amino Acids

193.1

Weight (kDa)

6.49

Isoelectric Point (pI)

38.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 30 - 146 2.9e-11 Sacsin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28020 AT3G48770 AT3G48770
fragaria_vesca FvH4_5g09020 FvH4_5g09020
malus_domestica MD06G1092400.v1.1 MD06G1134600.v1.1 MD06G1134700.v1.1 MD06G1134800.v1.1 MD06G1135200.v1.1 MD06G1135400.v1.1 MD06G1135500.v1.1 MD08G1137600.v1.1 MD15G1395100.v1.1
prunus_persica Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145600_v2.0.a1 Prupe.5G145800_v2.0.a1 Prupe.5G145900_v2.0.a1
pyrus_communis pycom06g12550 pycom06g12570 pycom06g12580 pycom06g12590 pycom06g12610 pycom06g12630 pycom06g12640 pycom06g12650 pycom08g06280
rosa_chinensis RchiOBHm_Chr6g0249321 RchiOBHm_Chr6g0275111 RchiOBHm_Chr7g0189191 RchiOBHm_Chr7g0189261
rosa_laevigata RLG00000004622 RLG00000004623 RLG00000008237 RLG00000008238 RLG00000023303 RLG00000035187
rosa_multiflora Rmu_co8294311.1_g000001 Rmu_co8389833.1_g000001 Rmu_sc0001044.1_g000004 Rmu_sc0001329.1_g000011 Rmu_sc0006012.1_g000010 Rmu_sc0007286.1_g000032 Rmu_sc0009360.1_g000016 Rmu_sc0011817.1_g000001 Rmu_sc0015050.1_g000001
rosa_roxburghii Rroxscaffold_1G00064000 Rroxscaffold_3G00265460 Rroxscaffold_3G00265580 Rroxscaffold_3G00265640 Rroxscaffold_3G00265690 Rroxscaffold_3G00265700 Rroxscaffold_7G00193620 Rroxscaffold_7G00204550 Rroxscaffold_7G00217350
rosa_rugosa Rorug01G0465500 Rorug06G0088500 Rorug06G0496700 Rorug06G0496700
rosa_samantha Rh1AG064800 Rh2BG015100 Rh2BG493500 Rh2DG017300 Rh2DG502500 Rh4BG208500 Rh5BG528500 Rh5CG552900 Rh6CG207800 Rh6DG197900 Rh6DG198000 Rh7AG101300 Rh7AG101400 Rh7AG101600 Rh7BG104100 Rh7BG104200 Rh7CG105600 Rh7CG105700 Rh7DG103200 Rh7DG103400
rosa_wichuraiana Rw1G007300 Rw5G034670 Rw7G008750 Rw7G008780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2349, 2945
AatII GACGTC 1 cut(s) 144
Acc65I GGTACC 1 cut(s) 4482
AccB1I GGYRCC 1 cut(s) 4482
AccBSI CCGCTC 1 cut(s) 1927
AccI GTMKAC 1 cut(s) 2189
AccII CGCG 2 cut(s) 234, 741
AciI CCGC 8 cut(s) 232, 673, 803, 1028, 1110, 1927, 2099, 3838
AclWI GGATC 9 cut(s) 99, 197, 1445, 2490, 2503, 2803, 4064, 4384, 4509
AcoI YGGCCR 1 cut(s) 2724
AcyI GRCGYC 3 cut(s) 17, 141, 4586
AfeI AGCGCT 3 cut(s) 3351, 4647, 4960
AfiI CCNNNNNNNGG 3 cut(s) 85, 1690, 2084
AflIII ACRYGT 1 cut(s) 840
AhlI ACTAGT 1 cut(s) 2026
AjnI CCWGG 5 cut(s) 99, 967, 2428, 3103, 3699
AjuI GAANNNNNNNTTGG 4 cut(s) 998, 1030, 4486, 4518
AloI GAACNNNNNNTCC 6 cut(s) 1286, 1318, 1401, 1433, 4065, 4097
Alw26I GTCTC 8 cut(s) 737, 1049, 2506, 2640, 2790, 3119, 3792, 4618
AlwI GGATC 9 cut(s) 99, 197, 1445, 2490, 2503, 2803, 4064, 4384, 4509
AlwNI CAGNNNCTG 2 cut(s) 1649, 4040
Aor51HI AGCGCT 3 cut(s) 3351, 4647, 4960
AoxI GGCC 4 cut(s) 102, 2438, 2724, 3848
ApeKI GCWGC 8 cut(s) 1668, 2231, 2234, 2642, 3257, 3393, 4046, 4676
AseI ATTAAT 1 cut(s) 1334
Asp700I GAANNNNTTC 4 cut(s) 162, 942, 2627, 4719
Asp718I GGTACC 1 cut(s) 4482
AspA2I CCTAGG 1 cut(s) 3635
AspLEI GCGC 4 cut(s) 1159, 3352, 4648, 4961
AspS9I GGNCC 3 cut(s) 333, 700, 3848
AsuC2I CCSGG 1 cut(s) 1754
AsuNHI GCTAGC 1 cut(s) 1849
AvaII GGWCC 2 cut(s) 333, 700
AvrII CCTAGG 1 cut(s) 3635
AxyI CCTNAGG 1 cut(s) 2991
BaeGI GKGCMC 1 cut(s) 1107
BaeI ACNNNNGTAYC 2 cut(s) 4424, 4457
BamHI GGATCC 1 cut(s) 2495
BanI GGYRCC 1 cut(s) 4482
BanII GRGCYC 3 cut(s) 256, 1287, 4122
BarI GAAGNNNNNNTAC 2 cut(s) 1257, 1289
BauI CACGAG 2 cut(s) 1317, 1725
BbsI GAAGAC 9 cut(s) 53, 183, 696, 935, 1769, 2771, 3086, 3155, 4449
BbvCI CCTCAGC 1 cut(s) 4488
BbvI GCAGC 8 cut(s) 1680, 2218, 2221, 2654, 3244, 3380, 4033, 4663
BceAI ACGGC 4 cut(s) 89, 258, 2756, 3633
BciT130I CCWGG 5 cut(s) 101, 969, 2430, 3105, 3701
BciVI GTATCC 2 cut(s) 1825, 2549
BclI TGATCA 7 cut(s) 405, 912, 1855, 2857, 3175, 4963, 4989
BcnI CCSGG 1 cut(s) 1754
BcoDI GTCTC 8 cut(s) 737, 1049, 2506, 2640, 2790, 3119, 3792, 4618
BcuI ACTAGT 1 cut(s) 2026
BfaI CTAG 6 cut(s) 1850, 2027, 2661, 3636, 5009, 5098
BfmI CTRYAG 2 cut(s) 246, 2790
BfoI RGCGCY 3 cut(s) 3353, 4649, 4962
BfuI GTATCC 2 cut(s) 1825, 2549
BglII AGATCT 1 cut(s) 4857
BisI GCNGC 9 cut(s) 674, 1669, 2232, 2235, 2643, 3258, 3394, 4047, 4677
BlnI CCTAGG 1 cut(s) 3635
BlpI GCTNAGC 1 cut(s) 414
BlsI GCNGC 9 cut(s) 675, 1670, 2233, 2236, 2644, 3259, 3395, 4048, 4678
BmcAI AGTACT 1 cut(s) 188
Bme1390I CCNGG 6 cut(s) 101, 969, 1754, 2430, 3105, 3701
Bme18I GGWCC 2 cut(s) 333, 700
BmgT120I GGNCC 3 cut(s) 333, 700, 3848
BmiI GGNNCC 6 cut(s) 255, 702, 2497, 3067, 4032, 4484
BmrFI CCNGG 6 cut(s) 101, 969, 1754, 2430, 3105, 3701
BmrI ACTGGG 1 cut(s) 2443
BmsI GCATC 8 cut(s) 763, 802, 1052, 3302, 3335, 3631, 3896, 4336
BmtI GCTAGC 1 cut(s) 1853
BmuI ACTGGG 1 cut(s) 2443
BpiI GAAGAC 9 cut(s) 53, 183, 696, 935, 1769, 2771, 3086, 3155, 4449
BplI GAGNNNNNCTC 2 cut(s) 57, 89
BpmI CTGGAG 4 cut(s) 240, 2216, 4168, 4905
Bpu10I CCTNAGC 2 cut(s) 1863, 4488
Bpu1102I GCTNAGC 1 cut(s) 414
BpuEI CTTGAG 7 cut(s) 233, 1115, 1972, 2231, 2924, 4033, 5072
BpuMI CCSGG 1 cut(s) 1754
BsaBI GATNNNNATC 1 cut(s) 1884
BsaHI GRCGYC 3 cut(s) 17, 141, 4586
BsaI GGTCTC 2 cut(s) 2790, 3792
BsaXI ACNNNNNCTCC 6 cut(s) 238, 268, 1286, 1316, 4065, 4095
Bsc4I CCNNNNNNNGG 3 cut(s) 85, 1690, 2084
Bse1I ACTGG 7 cut(s) 1277, 1481, 2199, 2449, 2762, 4185, 4319
Bse21I CCTNAGG 1 cut(s) 2991
Bse3DI GCAATG 5 cut(s) 263, 472, 484, 3329, 4416
Bse8I GATNNNNATC 1 cut(s) 1884
BseBI CCWGG 5 cut(s) 101, 969, 2430, 3105, 3701
BseJI GATNNNNATC 1 cut(s) 1884
BseLI CCNNNNNNNGG 3 cut(s) 85, 1690, 2084
BseMI GCAATG 5 cut(s) 263, 472, 484, 3329, 4416
BseMII CTCAG 9 cut(s) 428, 1814, 2381, 3005, 4502, 4692, 4880, 4929, 4974
BseNI ACTGG 7 cut(s) 1277, 1481, 2199, 2449, 2762, 4185, 4319
BseRI GAGGAG 4 cut(s) 901, 2579, 4671, 5093
BseSI GKGCMC 1 cut(s) 1107
BseX3I CGGCCG 1 cut(s) 2724
BseXI GCAGC 8 cut(s) 1680, 2218, 2221, 2654, 3244, 3380, 4033, 4663
BsgI GTGCAG 2 cut(s) 1687, 2274
Bsh1236I CGCG 2 cut(s) 234, 741
Bsh1285I CGRYCG 1 cut(s) 2727
BshFI GGCC 4 cut(s) 104, 2440, 2726, 3850
BshNI GGYRCC 1 cut(s) 4482
BsiEI CGRYCG 1 cut(s) 2727
BsiSI CCGG 3 cut(s) 120, 1753, 2723
BslFI GGGAC 5 cut(s) 2057, 2231, 2720, 3373, 3930
BslI CCNNNNNNNGG 3 cut(s) 85, 1690, 2084
BsmAI GTCTC 8 cut(s) 737, 1049, 2506, 2640, 2790, 3119, 3792, 4618
BsmBI CGTCTC 1 cut(s) 2506
BsmFI GGGAC 5 cut(s) 2057, 2231, 2720, 3373, 3930
BsmI GAATGC 7 cut(s) 175, 833, 2314, 2674, 3141, 3456, 4019
BsnI GGCC 4 cut(s) 104, 2440, 2726, 3850
Bso31I GGTCTC 2 cut(s) 2790, 3792
Bsp1286I GDGCHC 4 cut(s) 256, 1107, 1287, 4122
Bsp1407I TGTACA 2 cut(s) 3207, 4570
Bsp1720I GCTNAGC 1 cut(s) 414
Bsp19I CCATGG 2 cut(s) 3758, 4686
BspACI CCGC 8 cut(s) 232, 673, 803, 1028, 1110, 1927, 2099, 3838
BspANI GGCC 4 cut(s) 104, 2440, 2726, 3850
BspCNI CTCAG 9 cut(s) 427, 1813, 2380, 3004, 4501, 4693, 4879, 4930, 4975
BspFNI CGCG 2 cut(s) 234, 741
BspHI TCATGA 2 cut(s) 3178, 3738
BspLI GGNNCC 6 cut(s) 255, 702, 2497, 3067, 4032, 4484
BspOI GCTAGC 1 cut(s) 1853
BspPI GGATC 9 cut(s) 99, 197, 1445, 2490, 2503, 2803, 4064, 4384, 4509
BspQI GCTCTTC 1 cut(s) 3563
BspT107I GGYRCC 1 cut(s) 4482
BspTNI GGTCTC 2 cut(s) 2790, 3792
BsrBI CCGCTC 1 cut(s) 1927
BsrDI GCAATG 5 cut(s) 263, 472, 484, 3329, 4416
BsrGI TGTACA 2 cut(s) 3207, 4570
BsrI ACTGG 7 cut(s) 1277, 1481, 2199, 2449, 2762, 4185, 4319
BssNAI GTATAC 1 cut(s) 2190
BssNI GRCGYC 3 cut(s) 17, 141, 4586
BssSI CACGAG 2 cut(s) 1317, 1725
BssT1I CCWWGG 9 cut(s) 20, 704, 1468, 3312, 3446, 3635, 3758, 4350, 4686
Bst1107I GTATAC 1 cut(s) 2190
Bst2BI CACGAG 2 cut(s) 1317, 1725
Bst2UI CCWGG 5 cut(s) 101, 969, 2430, 3105, 3701
Bst6I CTCTTC 5 cut(s) 967, 3563, 4575, 4700, 4889
BstACI GRCGYC 3 cut(s) 17, 141, 4586
BstAUI TGTACA 2 cut(s) 3207, 4570
BstC8I GCNNGC 8 cut(s) 125, 739, 1341, 1851, 2383, 3646, 3958, 4275
BstDSI CCRYGG 2 cut(s) 3758, 4686
BstEII GGTNACC 1 cut(s) 1000
BstFNI CGCG 2 cut(s) 234, 741
BstH2I RGCGCY 3 cut(s) 3353, 4649, 4962
BstHHI GCGC 4 cut(s) 1159, 3352, 4648, 4961
BstMAI GTCTC 8 cut(s) 737, 1049, 2506, 2640, 2790, 3119, 3792, 4618
BstMCI CGRYCG 1 cut(s) 2727
BstMWI GCNNNNNNNGC 9 cut(s) 251, 483, 1674, 1713, 1859, 3299, 4498, 4592, 4654
BstNI CCWGG 5 cut(s) 101, 969, 2430, 3105, 3701
BstNSI RCATGY 6 cut(s) 755, 844, 1821, 2231, 2610, 4277
BstPI GGTNACC 1 cut(s) 1000
BstSCI CCNGG 6 cut(s) 99, 967, 1752, 2428, 3103, 3699
BstSFI CTRYAG 2 cut(s) 246, 2790
BstSLI GKGCMC 1 cut(s) 1107
BstUI CGCG 2 cut(s) 234, 741
BstV1I GCAGC 8 cut(s) 1680, 2218, 2221, 2654, 3244, 3380, 4033, 4663
BstV2I GAAGAC 9 cut(s) 53, 183, 696, 935, 1769, 2771, 3086, 3155, 4449
BstX2I RGATCY 7 cut(s) 91, 2495, 2795, 4056, 4376, 4514, 4857
BstXI CCANNNNNNTGG 3 cut(s) 268, 997, 4693
BstYI RGATCY 7 cut(s) 91, 2495, 2795, 4056, 4376, 4514, 4857
BstZ17I GTATAC 1 cut(s) 2190
BstZI CGGCCG 1 cut(s) 2724
Bsu36I CCTNAGG 1 cut(s) 2991
BsuI GTATCC 2 cut(s) 1825, 2549
BsuRI GGCC 4 cut(s) 104, 2440, 2726, 3850
BtgI CCRYGG 2 cut(s) 3758, 4686
BtsI GCAGTG 2 cut(s) 328, 408
BtsIMutI CAGTG 9 cut(s) 328, 408, 575, 714, 1488, 2192, 4038, 4192, 4312
Cac8I GCNNGC 8 cut(s) 125, 739, 1341, 1851, 2383, 3646, 3958, 4275
CaiI CAGNNNCTG 2 cut(s) 1649, 4040
CciI TCATGA 2 cut(s) 3178, 3738
CfoI GCGC 4 cut(s) 1159, 3352, 4648, 4961
Cfr13I GGNCC 3 cut(s) 333, 700, 3848
CseI GACGC 4 cut(s) 25, 744, 4052, 4575
EaeI YGGCCR 1 cut(s) 2724
EagI CGGCCG 1 cut(s) 2724
Eam1104I CTCTTC 5 cut(s) 967, 3563, 4575, 4700, 4889
EarI CTCTTC 5 cut(s) 967, 3563, 4575, 4700, 4889
EciI GGCGGA 1 cut(s) 1043
EclXI CGGCCG 1 cut(s) 2724
Eco130I CCWWGG 9 cut(s) 20, 704, 1468, 3312, 3446, 3635, 3758, 4350, 4686
Eco24I GRGCYC 3 cut(s) 256, 1287, 4122
Eco31I GGTCTC 2 cut(s) 2790, 3792
Eco32I GATATC 1 cut(s) 4935
Eco47I GGWCC 2 cut(s) 333, 700
Eco47III AGCGCT 3 cut(s) 3351, 4647, 4960
Eco52I CGGCCG 1 cut(s) 2724
Eco81I CCTNAGG 1 cut(s) 2991
Eco91I GGTNACC 1 cut(s) 1000
EcoO109I RGGNCCY 1 cut(s) 700
EcoO65I GGTNACC 1 cut(s) 1000
EcoRI GAATTC 6 cut(s) 872, 1912, 2164, 3182, 3866, 4318
EcoRII CCWGG 5 cut(s) 99, 967, 2428, 3103, 3699
EcoRV GATATC 1 cut(s) 4935
EcoT14I CCWWGG 9 cut(s) 20, 704, 1468, 3312, 3446, 3635, 3758, 4350, 4686
EcoT22I ATGCAT 4 cut(s) 982, 1511, 3058, 3295
EcoT38I GRGCYC 3 cut(s) 256, 1287, 4122
ErhI CCWWGG 9 cut(s) 20, 704, 1468, 3312, 3446, 3635, 3758, 4350, 4686
Esp3I CGTCTC 1 cut(s) 2506
FalI AAGNNNNNCTT 8 cut(s) 946, 978, 1077, 1109, 3809, 3841, 4486, 4518
FaqI GGGAC 5 cut(s) 2057, 2231, 2720, 3373, 3930
FauI CCCGC 1 cut(s) 2092
FauNDI CATATG 1 cut(s) 1945
FbaI TGATCA 7 cut(s) 405, 912, 1855, 2857, 3175, 4963, 4989
FblI GTMKAC 1 cut(s) 2189
Fnu4HI GCNGC 9 cut(s) 674, 1669, 2232, 2235, 2643, 3258, 3394, 4047, 4677
FriOI GRGCYC 3 cut(s) 256, 1287, 4122
Fsp4HI GCNGC 9 cut(s) 674, 1669, 2232, 2235, 2643, 3258, 3394, 4047, 4677
FspBI CTAG 6 cut(s) 1850, 2027, 2661, 3636, 5009, 5098
GlaI GCGC 4 cut(s) 1158, 3351, 4647, 4960
GluI GCNGC 9 cut(s) 674, 1669, 2232, 2235, 2643, 3258, 3394, 4047, 4677
GsuI CTGGAG 4 cut(s) 240, 2216, 4168, 4905
HaeII RGCGCY 3 cut(s) 3353, 4649, 4962
HaeIII GGCC 4 cut(s) 104, 2440, 2726, 3850
HapII CCGG 3 cut(s) 120, 1753, 2723
HgaI GACGC 4 cut(s) 25, 744, 4052, 4575
HhaI GCGC 4 cut(s) 1159, 3352, 4648, 4961
Hin1I GRCGYC 3 cut(s) 17, 141, 4586
Hin6I GCGC 4 cut(s) 1157, 3350, 4646, 4959
HinP1I GCGC 4 cut(s) 1157, 3350, 4646, 4959
HincII GTYRAC 2 cut(s) 2339, 2509
HindII GTYRAC 2 cut(s) 2339, 2509
HindIII AAGCTT 3 cut(s) 1394, 1931, 2204
HpaII CCGG 3 cut(s) 120, 1753, 2723
Hpy166II GTNNAC 9 cut(s) 145, 336, 470, 1555, 2002, 2190, 2339, 2509, 4130
Hpy8I GTNNAC 9 cut(s) 145, 336, 470, 1555, 2002, 2190, 2339, 2509, 4130
Hpy99I CGWCG 4 cut(s) 10, 13, 3158, 3590
HpyCH4IV ACGT 2 cut(s) 141, 940
HpyF10VI GCNNNNNNNGC 9 cut(s) 251, 483, 1674, 1713, 1859, 3299, 4498, 4592, 4654
HpySE526I ACGT 2 cut(s) 141, 940
Hsp92I GRCGYC 3 cut(s) 17, 141, 4586
HspAI GCGC 4 cut(s) 1157, 3350, 4646, 4959
KpnI GGTACC 1 cut(s) 4486
Ksp22I TGATCA 7 cut(s) 405, 912, 1855, 2857, 3175, 4963, 4989
LguI GCTCTTC 1 cut(s) 3563
LmnI GCTCC 8 cut(s) 259, 1294, 2266, 2566, 3065, 3192, 3390, 4684
Lsp1109I GCAGC 8 cut(s) 1680, 2218, 2221, 2654, 3244, 3380, 4033, 4663
LweI GCATC 8 cut(s) 763, 802, 1052, 3302, 3335, 3631, 3896, 4336
MaeI CTAG 6 cut(s) 1850, 2027, 2661, 3636, 5009, 5098
MaeII ACGT 2 cut(s) 141, 940
MbiI CCGCTC 1 cut(s) 1927
MfeI CAATTG 5 cut(s) 726, 930, 1585, 2577, 4133
MflI RGATCY 7 cut(s) 91, 2495, 2795, 4056, 4376, 4514, 4857
MhlI GDGCHC 4 cut(s) 256, 1107, 1287, 4122
MlyI GAGTC 7 cut(s) 305, 1247, 1640, 1765, 3355, 3763, 3806
MmeI TCCRAC 2 cut(s) 307, 590
Mph1103I ATGCAT 4 cut(s) 982, 1511, 3058, 3295
MroXI GAANNNNTTC 4 cut(s) 162, 942, 2627, 4719
MslI CAYNNNNRTG 7 cut(s) 477, 995, 1613, 3005, 3966, 4024, 4691
MspA1I CMGCKG 2 cut(s) 803, 4757
MspI CCGG 3 cut(s) 120, 1753, 2723
MspR9I CCNGG 6 cut(s) 101, 969, 1754, 2430, 3105, 3701
MunI CAATTG 5 cut(s) 726, 930, 1585, 2577, 4133
Mva1269I GAATGC 7 cut(s) 175, 833, 2314, 2674, 3141, 3456, 4019
MvaI CCWGG 5 cut(s) 101, 969, 2430, 3105, 3701
MvnI CGCG 2 cut(s) 234, 741
MwoI GCNNNNNNNGC 9 cut(s) 251, 483, 1674, 1713, 1859, 3299, 4498, 4592, 4654
NciI CCSGG 1 cut(s) 1754
NcoI CCATGG 2 cut(s) 3758, 4686
NdeI CATATG 1 cut(s) 1945
NheI GCTAGC 1 cut(s) 1849
NlaIV GGNNCC 6 cut(s) 255, 702, 2497, 3067, 4032, 4484
NmeAIII GCCGAG 1 cut(s) 4647
NmuCI GTSAC 7 cut(s) 718, 1000, 1604, 1768, 2609, 2961, 4587
NsiI ATGCAT 4 cut(s) 982, 1511, 3058, 3295
NspI RCATGY 6 cut(s) 755, 844, 1821, 2231, 2610, 4277
PaeI GCATGC 1 cut(s) 4277
PagI TCATGA 2 cut(s) 3178, 3738
PciI ACATGT 1 cut(s) 840
PciSI GCTCTTC 1 cut(s) 3563
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PctI GAATGC 7 cut(s) 175, 833, 2314, 2674, 3141, 3456, 4019
PdmI GAANNNNTTC 4 cut(s) 162, 942, 2627, 4719
PfoI TCCNGGA 1 cut(s) 3699
PkrI GCNGC 9 cut(s) 675, 1670, 2233, 2236, 2644, 3259, 3395, 4048, 4678
PleI GAGTC 7 cut(s) 305, 1246, 1640, 1765, 3355, 3763, 3805
PpsI GAGTC 7 cut(s) 305, 1246, 1640, 1765, 3355, 3763, 3805
PpuMI RGGWCCY 1 cut(s) 700
PscI ACATGT 1 cut(s) 840
PshBI ATTAAT 1 cut(s) 1334
PsiI TTATAA 2 cut(s) 2349, 2945
Psp5II RGGWCCY 1 cut(s) 700
Psp6I CCWGG 5 cut(s) 99, 967, 2428, 3103, 3699
PspEI GGTNACC 1 cut(s) 1000
PspGI CCWGG 5 cut(s) 99, 967, 2428, 3103, 3699
PspN4I GGNNCC 6 cut(s) 255, 702, 2497, 3067, 4032, 4484
PspPI GGNCC 3 cut(s) 333, 700, 3848
PspPPI RGGWCCY 1 cut(s) 700
PstNI CAGNNNCTG 2 cut(s) 1649, 4040
PsuI RGATCY 7 cut(s) 91, 2495, 2795, 4056, 4376, 4514, 4857
PvuII CAGCTG 1 cut(s) 4757
RseI CAYNNNNRTG 7 cut(s) 477, 995, 1613, 3005, 3966, 4024, 4691
SapI GCTCTTC 1 cut(s) 3563
SatI GCNGC 9 cut(s) 674, 1669, 2232, 2235, 2643, 3258, 3394, 4047, 4677
Sau96I GGNCC 3 cut(s) 333, 700, 3848
ScaI AGTACT 1 cut(s) 188
SchI GAGTC 7 cut(s) 305, 1247, 1640, 1765, 3355, 3763, 3806
ScrFI CCNGG 6 cut(s) 101, 969, 1754, 2430, 3105, 3701
SduI GDGCHC 4 cut(s) 256, 1107, 1287, 4122
SfaNI GCATC 8 cut(s) 763, 802, 1052, 3302, 3335, 3631, 3896, 4336
SfcI CTRYAG 2 cut(s) 246, 2790
SinI GGWCC 2 cut(s) 333, 700
SmiMI CAYNNNNRTG 7 cut(s) 477, 995, 1613, 3005, 3966, 4024, 4691
SmlI CTYRAG 7 cut(s) 212, 1094, 1987, 2210, 2903, 4012, 5087
SmoI CTYRAG 7 cut(s) 212, 1094, 1987, 2210, 2903, 4012, 5087
SpeI ACTAGT 1 cut(s) 2026
SphI GCATGC 1 cut(s) 4277
SsiI CCGC 8 cut(s) 232, 673, 803, 1028, 1110, 1927, 2099, 3838
SspI AATATT 2 cut(s) 307, 2597
SspMI CTAG 6 cut(s) 1850, 2027, 2661, 3636, 5009, 5098
StyD4I CCNGG 6 cut(s) 99, 967, 1752, 2428, 3103, 3699
StyI CCWWGG 9 cut(s) 20, 704, 1468, 3312, 3446, 3635, 3758, 4350, 4686
TaiI ACGT 2 cut(s) 144, 943
TaqI TCGA 8 cut(s) 629, 1712, 3156, 3274, 3588, 4051, 4307, 4537
TatI WGTACW 5 cut(s) 186, 1554, 3207, 3552, 4570
TauI GCSGC 1 cut(s) 676
TscAI CASTG 9 cut(s) 328, 415, 582, 721, 1488, 2199, 4045, 4192, 4319
TseFI GTSAC 7 cut(s) 718, 1000, 1604, 1768, 2609, 2961, 4587
TseI GCWGC 8 cut(s) 1668, 2231, 2234, 2642, 3257, 3393, 4046, 4676
Tsp45I GTSAC 7 cut(s) 718, 1000, 1604, 1768, 2609, 2961, 4587
TspGWI ACGGA 3 cut(s) 58, 101, 2488
TspRI CASTG 9 cut(s) 328, 415, 582, 721, 1488, 2199, 4045, 4192, 4319
VpaK11BI GGWCC 2 cut(s) 333, 700
VspI ATTAAT 1 cut(s) 1334
XceI RCATGY 6 cut(s) 755, 844, 1821, 2231, 2610, 4277
XcmI CCANNNNNNNNNTGG 1 cut(s) 4074
XmaJI CCTAGG 1 cut(s) 3635
XmiI GTMKAC 1 cut(s) 2189
XmnI GAANNNNTTC 4 cut(s) 162, 942, 2627, 4719
XspI CTAG 6 cut(s) 1850, 2027, 2661, 3636, 5009, 5098
ZraI GACGTC 1 cut(s) 142
ZrmI AGTACT 1 cut(s) 188
Zsp2I ATGCAT 4 cut(s) 982, 1511, 3058, 3295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.