pycom06g12550

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0000526
Physical Location & Seq
Forward (+)
17681927 .. 17692764
10838 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g12550.3

Sequence Viewer

Length: 5124 bp
ATGGCGACGCCGAGAGAGCACATAGAAGAAATACGCAAGGACTTCTCCATAGGAGAAGAAAAACACCGTTTGACTAAGGGTTTTCACCAGGCCGTAAAGTATCTCTCTGCTGAACTCTACGCTAAGGATGTTCACTTCCTCATGGAGCTCCTCCAGAATGCAGAAGATAATGATTACCCAGAAGGGGTTGATCGATCACTTGAGTTTGTCATAACTTCGCGGGATATAACAGCCACGGGGGCTCCTGCTACTTTAATTGTTTTCAACAATGAGAAGGGGTTTTCACCAGACAACATCGAGGCCATTTGCAATGTTGGACTTTCCACCAAGAAAGGCAACAGGAAGCGCGGCTACATTGGGGAGAAAGGTATTGGGTTCAAAAGCGTGTTTCTCATTACTGCGCAACCTTACATTTTCAGTAACAGATATCAAATAAGGTTCAGTGAAGAGCCCTGTGTGCACTGCAATGTTGGCTATATAGTTCCGGAATGGGTTGATGCAAACCCTACTCTTTCAGACATAAAACAGATGTATGGTTCTGGTTCTGCCCTTCCAAACACAACCCTGATCTTACCTTTGAAGGCGGACAAGGTTGAAGCTGTGAAGCAACAGCTATCTAGCATGCAACCGGAAATTCTCTTGTTCTTATCAAAGATTAAGCGGCTCTCGGTTAGGGAAGACAATGAGGATCCAAGGCACAACACTGTAAGCGCGATTGCAATATCAAGTGATACTAATTCTGACACCAGGAAGGACATGGAATCCTACACACTCCACCTTTCTGCGAAAGAAAATGGCTGCAAGTTTGATAATGAATGCGGCTATTATATGTGGAAGCAGAAGTTTCCTGTCAAGCCGGAGTGCAGAGTGGATGGGAGAAATGATGTAGATGAATGGGTGATCACGCTTGCATTTCCAATTGGAGAGCGCCTTCGTAGAGGAACAAACACATCTCCTGGAGTCTATGCATTCCTTCCCACCGAGATGGTGACAAACTTTCCCTTTATAATTCAAGCCGATTTTCTTCTTGCCTCATCGAGGGAAACAATTCTTCTGGACAGCAAATGGAATAAGGGGATTCTCGATTGCGTGCCAACTGCATTTGTGAATGCATTTATCTCGCTTGTCCGATCTAAAGAAGATGCTCCAGTGTCTAGCTTGACTAGTATGTTCAAATTTCTGCCTGTTCAGAGCTCTTCTTATGAAGGATTGACTGTTGTCAGGGAGTCTATAAAGGCAAAGATTGTTGAAGAAAGCATTGTCCCTAGTGAGCCTCACGAGGAGCGGAAGGTATTTTATAAACCCTGTGAAGTCGGTAGGTTAATGCCTGCCTTTTGGAATATTTTGCATAAAGCGAAGAAGCTAGGGGTAAGCTTGGTTAATCTCTCGTCTCATGGAAAACCAGTTCTGGCATATGCCCTTGATGATGTCGAGTATAACGAGATACTGAATTTCCTGGGAGTTGAAGCCGTGAACAATGAATGGTATGCAACATGCATCTGCGGTACATCTAATCTTATAACTGGGGTGCTGGAAGATGTCTATTTGGAGCTTCTACTTTTCATTTCTGAATTTTGGGGGTCCAAATTTTCAAGCACCAGCATCAAGAATATACCGCTCATAAAATATGTGGGTTATCGAAACAAATCTCTGTGCAGCATAAGCGCAATCCAGAAAGGCGAAAGCAAAGTTTGCTTATCAAGTGATTCTTGTCATGTTTCATGGCTGATTGACTGGAACATGGAGTTTGCATCTGTTTCCAATGTTCTCTTTATGCCAGAGATCACACAAGAAGCTCTCGGATTGTGTTCTAGGAAGGAGACGTTGAAGAAGTGGCTTTTGGACCAAGTCAAGGTTGGTTCTGTAAGTATATGTGACTATGCAGTCAATCTCTTTGATCAGTCACTTTGTGAAAGGAAGGTTGCTATTGCATTCGCCCACTTTTTATACCAGTCTTTGAGCAAGGGATATATCTTATCTTGGGAAGCTGTTAATTTGTGTGCACTGATGCCGTTGGTGGATAAGTATGGCAACATCACCAGCACTAGGAAGGGAGTTCTTGTTCCAGCTAATGGAAGCAAATGGGCAGGATTGACCGACTCTAATCTGTGGAGAAGAGAAGGTTATGTTGAGTTGAACGAAGACTATTTGGATTCCGGTCATTTTGCTGGTAGCTTTACACAACAGAAGGAGCTCCTTGGGTTTCTAAAAGATCATGCTAAAGCCTCGGATATCCCAAATGTTTGTGCTCCCAGCGATGGTATATCATCTTTATCTGCACCACTCAATAAGCAAAATGTATTCTTGCTTTTGGACTGGATTCGCCAGCTCATGCATAAAGCTAGCATCCCTCAGAAGTTCTTGATGTGTGTAAAAGAAGGTAGCTGGCTGAAAGTTACTTTGAATGGTTCTCCTGGTTTCAGGCCACCATCGCAGTCATTTCTTCTCAAGTCATCATCGGGAAACCTTTTGCAGAATAGCTCTGTTTTTGTTGATATACCGTTGATTGATCAAAGTCATTATGATGGAAAGATTAACGAATACAAGGAGGAGTTGAAAAAAATTGGAGTCAGGTTTGAGTTTGGAGAAGCGTGTGAATATATGGGGAAGCATCTCATGTCTCTTGCTGCTGCTTCCTCTTTAACCCGTGGCAATGTATTTTCTATACTACGTTTCATCAGATTTTTGAGAGAGAAACTTCTTCCACCAGCCGATTTTATCAGCAGTATTAAAGATGGTCAGTGGCTTAAAACTTCCCTTGGCTTCAGGTCTCCGGATGGATCTGTCTTGTCGGATGATGAGTGGAAAGTTGCTTCGCAAATTAGCGACATTCCCTTCATAGATGAAGCATTCTATGGTAAAGAAGAAATCTGTCAGTTCAAAACAGAACTTGAGTTGCTTGGCGTGGCAGTCTCATTCAGCAAATGCTACCAACTGATAATTGACAACCTGAAGTCACATTCTTGCTTGACTTCTTGGACACCTGAGGTTCTTCTATTGATGCTTAAATGCATGTGTCTTTCGAGCTCATCTGAGAAACTTGTCAGTGCTTTGAAAGGATCGAATTGCCTAAAGACAAAAACTGGTTACAAGCGTCCAGAGGAATGTCTCCTGTTCGACAAGGATTGGGGATGCATTCTTCAGGTCTTCAGTGGTTTCCCATTGATTGATCACGATTTCTATGGAGACAGAATATTTTCCTTCAGAAATGAGTTGAAGAAAACTGGGGTGGTGGTTGGTTTTGAGGATTCCGCAAACGTATTTGCCCATTATTTCAAGCAGCATGCATCTTCGACTTCCATTTCAAAAGAAAATGTTGCATCAATTCTGTTGTGTTACAGAACACTGAATGTAACTCCATTTAAATTTCCTGCAGATCTTACGAGCTGCATTTGCGAGATGAAGTGGCTGCGTACTCGCCTTGGTGATCATAGATCTCCCAAACAGTGCATTCTCTCTGGTCCCGAGTGGGATTCCATCTCTTCCATATGTCTTCTTCCATTCATTGATGATTCTGAAAATCATTATGGAAAAAACATTCATGAATTTAAGAACGAACTGAAGATCTTGGGGGTGGTTGTGGAATTCAAGGACGGAGTCAAGTTTGTGGAATCTTGTCTGTATCTTCCTCAGGATCCGACCTGCATTTCTCGGGAAAATGCATTAGCATTGCTTGAATGTATACAGATCTTATTGCAGGAGAAAGCCTACTCCCTTCCGGAGGCTTTCAGGGGAAAACTTTCTCAAGCATGGTTGAAGACTCATGTTGGTTACCGGTCTCCTAACCAGTGCTTGTTGTTTGATTCGGAAACGCATTTAAAGCAGACTGATGGGCCTTTCATCGATGAAGAATTTTATGGTTCTAAAATTACAACCTACAGAAAGGAGCTCGCTGCAATTGGAGTGATTGTCGATGTGAAAAAGGGATGTGCCTTGATTGCTAGGCACCTTGATCTACAGGATGAGTTTTCGACATTTGTTCGAGTATATAGTTACTTGAGTGAGTCTGAGTGGGTTCCACTGACAGATGGAGAGGCTCCCCGAAGGATCTGGGTTCCGAAAGAAAATAAAAATGGTGAGTGGGTTAGTGCAGATGAATGTGTTATGCATGACAAGGATGGTCTGTTTGGTTCGCAGTTAACTGTTTTGGAGAAACACTATGATCAAAAGTTGTTTGGTTTCTTCTCTTCTGCTTTTGGAATAAGATGCCGTCCTTCTGTTGATGATTACTTGAATCTCTGGAAAGTTTGGGAAAGTTCTGAAAGCGAATTGTTGCTTGATCAATGCTGCATGTTCTGGCTTTATGTTTTGAAGCACTGGAATTTAAAAACGGAGAAAAGTCTCGCTGATGCCGTGGTGAAAGTACCGGTTTATTCTGGTTCAGGCAAAATCTTGTTGTGCAACAAGGAGGACGTTTTCATTGCTGATGACCTTCAGCTGCAGTGTCTTTTTGAACAGTCTTCACAGTCAATATTTGTTTGGTACCCTCAGCCAAGCTTGGCCTCCTTGCCTCGAACTAAGCTGCTTGAAATGTACAAGAAAATTGGTGTTCGCACTATTTCAGAATCTGTGCAGAAGGAAGAAGTTTCTCTTGCAAATGATGCTGAGCTACAAATAATCCCAAGGGAAAGATTGTTCGGAAAAGCACTGCTGAGGCTGATTCTTGGATTTCTTGCTGGTCCTCCCATTGAAATGGAGGTGGAGAAGAGGCGGAAAGCTGTCCAAGGTCTTGTAGACGTTGCTGTATTGGAGACACCAGAACCAATCACTGTACGCTATGATCTGCCACTATCTTCTGGGGAAGTTTTGAATGTGAGAGGCACACGAAAGATACGGTGGGACAGGAAGAATTCCAAATGCTTCATCCAGATGGACAAATCTGGCCGGCAGAAGAAGACCGTTGAGTTTGCTACATATTTTGCCGAAGCAATCTCTGAGTGTGTTCTGTGGGAGTTCATTGAACTTATACCTGCACTTTCGGAGCTGATCGATCATCGATCCTGCGGGTTGGCATTCGTGCTAGAATTCAATGAGGAAGATGTTGATTTCTTGATGAAGTCGAAGAATTTGCAGATCTTCGTTGAGGATGTCGAGTTTCTGAATTCTGCCTGCCCATCTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1708

Amino Acids

193.07

Weight (kDa)

5.85

Isoelectric Point (pI)

45.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28020 AT3G48770 AT3G48770
fragaria_vesca FvH4_5g09020 FvH4_5g09020
malus_domestica MD06G1092400.v1.1 MD06G1134600.v1.1 MD06G1134700.v1.1 MD06G1134800.v1.1 MD06G1135200.v1.1 MD06G1135400.v1.1 MD06G1135500.v1.1 MD08G1137600.v1.1 MD15G1395100.v1.1
prunus_persica Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145600_v2.0.a1 Prupe.5G145800_v2.0.a1 Prupe.5G145900_v2.0.a1
pyrus_communis pycom06g12550 pycom06g12570 pycom06g12580 pycom06g12590 pycom06g12610 pycom06g12630 pycom06g12640 pycom06g12650 pycom08g06280
rosa_chinensis RchiOBHm_Chr6g0249321 RchiOBHm_Chr6g0275111 RchiOBHm_Chr7g0189191 RchiOBHm_Chr7g0189261
rosa_laevigata RLG00000004622 RLG00000004623 RLG00000008237 RLG00000008238 RLG00000023303 RLG00000035187
rosa_multiflora Rmu_co8294311.1_g000001 Rmu_co8389833.1_g000001 Rmu_sc0001044.1_g000004 Rmu_sc0001329.1_g000011 Rmu_sc0006012.1_g000010 Rmu_sc0007286.1_g000032 Rmu_sc0009360.1_g000016 Rmu_sc0011817.1_g000001 Rmu_sc0015050.1_g000001
rosa_roxburghii Rroxscaffold_1G00064000 Rroxscaffold_3G00265460 Rroxscaffold_3G00265580 Rroxscaffold_3G00265640 Rroxscaffold_3G00265690 Rroxscaffold_3G00265700 Rroxscaffold_7G00193620 Rroxscaffold_7G00204550 Rroxscaffold_7G00217350
rosa_rugosa Rorug01G0465500 Rorug06G0088500 Rorug06G0496700 Rorug06G0496700
rosa_samantha Rh1AG064800 Rh2BG015100 Rh2BG493500 Rh2DG017300 Rh2DG502500 Rh4BG208500 Rh5BG528500 Rh5CG552900 Rh6CG207800 Rh6DG197900 Rh6DG198000 Rh7AG101300 Rh7AG101400 Rh7AG101600 Rh7BG104100 Rh7BG104200 Rh7CG105600 Rh7CG105700 Rh7DG103200 Rh7DG103400
rosa_wichuraiana Rw1G007300 Rw5G034670 Rw7G008750 Rw7G008780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1007, 1299, 1520
AasI GACNNNNNNGTC 1 cut(s) 1883
Acc16I TGCGCA 1 cut(s) 402
Acc36I ACCTGC 2 cut(s) 3650, 4978
Acc65I GGTACC 1 cut(s) 4482
AccB1I GGYRCC 2 cut(s) 3945, 4482
AccB7I CCANNNNNTGG 1 cut(s) 2072
AccBSI CCGCTC 2 cut(s) 1285, 1618
AccI GTMKAC 2 cut(s) 3682, 4734
AccII CGCG 3 cut(s) 220, 348, 713
AccIII TCCGGA 3 cut(s) 484, 2773, 3718
AclWI GGATC 8 cut(s) 683, 696, 2788, 3067, 3629, 3642, 4055, 4992
AcoI YGGCCR 1 cut(s) 4882
AcuI CTGAAG 7 cut(s) 2749, 2972, 3125, 3133, 3187, 3581, 4418
AcyI GRCGYC 1 cut(s) 8
AdeI CACNNNGTG 1 cut(s) 1910
AfaI GTAC 6 cut(s) 1507, 3415, 4365, 4484, 4535, 4773
AfiI CCNNNNNNNGG 7 cut(s) 184, 1038, 1852, 2046, 2072, 2258, 3721
AgeI ACCGGT 2 cut(s) 3774, 4366
AhlI ACTAGT 1 cut(s) 1163
AjnI CCWGG 5 cut(s) 87, 746, 955, 1455, 2413
AjuI GAANNNNNNNTTGG 2 cut(s) 1823, 1855
AloI GAACNNNNNNTCC 6 cut(s) 1389, 1421, 2046, 2078, 4619, 4651
Alw21I GWGCWC 9 cut(s) 21, 150, 462, 1196, 2005, 2196, 2251, 3029, 3891
Alw44I GTGCAC 2 cut(s) 458, 2001
AlwI GGATC 8 cut(s) 683, 696, 2788, 3067, 3629, 3642, 4055, 4992
AlwNI CAGNNNCTG 1 cut(s) 4568
Ama87I CYCGRG 2 cut(s) 3464, 3651
Aor13HI TCCGGA 3 cut(s) 484, 2773, 3718
AoxI GGCC 6 cut(s) 90, 300, 2423, 3833, 4500, 4882
ApaLI GTGCAC 2 cut(s) 458, 2001
AsiGI ACCGGT 2 cut(s) 3774, 4366
Asp700I GAANNNNTTC 3 cut(s) 1047, 3196, 3739
Asp718I GGTACC 1 cut(s) 4482
AspLEI GCGC 5 cut(s) 348, 403, 713, 930, 1667
AspS9I GGNCC 5 cut(s) 1581, 1843, 3461, 3833, 4679
AsuHPI GGTGA 8 cut(s) 77, 276, 910, 1000, 2029, 3437, 4088, 4369
AsuNHI GCTAGC 1 cut(s) 2342
AvaI CYCGRG 2 cut(s) 3464, 3651
AvaII GGWCC 4 cut(s) 1581, 1843, 3461, 4679
AxyI CCTNAGG 2 cut(s) 2985, 3630
BaeGI GKGCMC 2 cut(s) 462, 2005
BamHI GGATCC 2 cut(s) 688, 3634
BanI GGYRCC 2 cut(s) 3945, 4482
BanII GRGCYC 7 cut(s) 150, 244, 453, 1196, 2196, 3029, 3891
BarI GAAGNNNNNNTAC 2 cut(s) 335, 367
BauI CACGAG 1 cut(s) 1277
BbsI GAAGAC 7 cut(s) 684, 2148, 3139, 3485, 3764, 4452, 4901
Bbv12I GWGCWC 9 cut(s) 21, 150, 462, 1196, 2005, 2196, 2251, 3029, 3891
BbvCI CCTCAGC 2 cut(s) 4488, 4652
BceAI ACGGC 5 cut(s) 77, 1454, 1996, 4194, 4337
BcgI CGANNNNNNTGC 4 cut(s) 1101, 1135, 5050, 5084
BciT130I CCWGG 5 cut(s) 89, 748, 957, 1457, 2415
BclI TGATCA 7 cut(s) 900, 1897, 2509, 3169, 3427, 4162, 4279
BcuI ACTAGT 1 cut(s) 1163
BfmI CTRYAG 4 cut(s) 3372, 3877, 3956, 4439
BfoI RGCGCY 1 cut(s) 931
BfuAI ACCTGC 2 cut(s) 3650, 4978
BglI GCCNNNNNGGC 1 cut(s) 239
BglII AGATCT 5 cut(s) 3376, 3434, 3564, 3687, 5073
BlpI GCTNAGC 1 cut(s) 4605
Bme1390I CCNGG 5 cut(s) 89, 748, 957, 1457, 2415
Bme18I GGWCC 4 cut(s) 1581, 1843, 3461, 4679
BmeT110I CYCGRG 2 cut(s) 3464, 3651
BmgT120I GGNCC 5 cut(s) 1581, 1843, 3461, 3833, 4679
BmrFI CCNGG 5 cut(s) 89, 748, 957, 1457, 2415
BmrI ACTGGG 2 cut(s) 1533, 3234
BmtI GCTAGC 1 cut(s) 2346
BmuI ACTGGG 2 cut(s) 1533, 3234
BoxI GACNNNNGTC 1 cut(s) 1217
BpiI GAAGAC 7 cut(s) 684, 2148, 3139, 3485, 3764, 4452, 4901
BpmI CTGGAG 3 cut(s) 137, 978, 1131
Bpu10I CCTNAGC 3 cut(s) 123, 4488, 4652
Bpu1102I GCTNAGC 1 cut(s) 4605
BpuEI CTTGAG 5 cut(s) 221, 2432, 2912, 3729, 4018
Bsa29I ATCGAT 4 cut(s) 193, 3843, 4989, 4996
BsaHI GRCGYC 1 cut(s) 8
BsaI GGTCTC 2 cut(s) 2775, 3783
BsaWI WCCGGW 7 cut(s) 484, 628, 2156, 2773, 3718, 3774, 4366
BsaXI ACNNNNNCTCC 6 cut(s) 226, 256, 2046, 2076, 3894, 3924
Bsc4I CCNNNNNNNGG 7 cut(s) 184, 1038, 1852, 2046, 2072, 2258, 3721
Bse118I RCCGGY 3 cut(s) 3774, 4366, 4884
Bse21I CCTNAGG 2 cut(s) 2985, 3630
Bse3DI GCAATG 5 cut(s) 316, 472, 2659, 3668, 4419
BseAI TCCGGA 3 cut(s) 484, 2773, 3718
BseBI CCWGG 5 cut(s) 89, 748, 957, 1457, 2415
BseCI ATCGAT 4 cut(s) 193, 3843, 4989, 4996
BseLI CCNNNNNNNGG 7 cut(s) 184, 1038, 1852, 2046, 2072, 2258, 3721
BseMI GCAATG 5 cut(s) 316, 472, 2659, 3668, 4419
BseMII CTCAG 9 cut(s) 2366, 2976, 3024, 3644, 3999, 4502, 4596, 4643, 4926
BseRI GAGGAG 3 cut(s) 140, 1295, 2564
BseSI GKGCMC 2 cut(s) 462, 2005
BseYI CCCAGC 1 cut(s) 2252
BsgI GTGCAG 6 cut(s) 883, 1675, 2262, 4110, 4592, 4956
Bsh1236I CGCG 3 cut(s) 220, 348, 713
BshFI GGCC 6 cut(s) 92, 302, 2425, 3835, 4502, 4884
BshNI GGYRCC 2 cut(s) 3945, 4482
BshTI ACCGGT 2 cut(s) 3774, 4366
BshVI ATCGAT 4 cut(s) 193, 3843, 4989, 4996
BsiHKAI GWGCWC 9 cut(s) 21, 150, 462, 1196, 2005, 2196, 2251, 3029, 3891
BsiHKCI CYCGRG 2 cut(s) 3464, 3651
BsiSI CCGG 9 cut(s) 485, 629, 857, 2157, 2774, 3719, 3775, 4367, 4885
BslFI GGGAC 3 cut(s) 1247, 3447, 4853
BslI CCNNNNNNNGG 7 cut(s) 184, 1038, 1852, 2046, 2072, 2258, 3721
BsmBI CGTCTC 2 cut(s) 1395, 1814
BsmFI GGGAC 3 cut(s) 1247, 3447, 4853
BsmI GAATGC 9 cut(s) 163, 821, 968, 1114, 1931, 2849, 3135, 3450, 5012
BsnI GGCC 6 cut(s) 92, 302, 2425, 3835, 4502, 4884
Bso31I GGTCTC 2 cut(s) 2775, 3783
BsoBI CYCGRG 2 cut(s) 3464, 3651
Bsp13I TCCGGA 3 cut(s) 484, 2773, 3718
Bsp1407I TGTACA 1 cut(s) 4533
Bsp1720I GCTNAGC 1 cut(s) 4605
BspANI GGCC 6 cut(s) 92, 302, 2425, 3835, 4502, 4884
BspCNI CTCAG 9 cut(s) 2365, 2977, 3025, 3643, 4000, 4501, 4597, 4644, 4927
BspDI ATCGAT 4 cut(s) 193, 3843, 4989, 4996
BspEI TCCGGA 3 cut(s) 484, 2773, 3718
BspFNI CGCG 3 cut(s) 220, 348, 713
BspHI TCATGA 1 cut(s) 3541
BspMAI CTGCAG 2 cut(s) 3376, 4443
BspMI ACCTGC 2 cut(s) 3650, 4978
BspOI GCTAGC 1 cut(s) 2346
BspPI GGATC 8 cut(s) 683, 696, 2788, 3067, 3629, 3642, 4055, 4992
BspQI GCTCTTC 2 cut(s) 441, 1201
BspT107I GGYRCC 2 cut(s) 3945, 4482
BspTNI GGTCTC 2 cut(s) 2775, 3783
BsrBI CCGCTC 2 cut(s) 1285, 1618
BsrDI GCAATG 5 cut(s) 316, 472, 2659, 3668, 4419
BsrFI RCCGGY 3 cut(s) 3774, 4366, 4884
BsrGI TGTACA 1 cut(s) 4533
BssAI RCCGGY 3 cut(s) 3774, 4366, 4884
BssNAI GTATAC 1 cut(s) 3683
BssNI GRCGYC 1 cut(s) 8
BssSI CACGAG 1 cut(s) 1277
BssT1I CCWWGG 6 cut(s) 692, 2197, 2758, 3421, 4622, 4723
Bst1107I GTATAC 1 cut(s) 3683
Bst2BI CACGAG 1 cut(s) 1277
Bst2UI CCWGG 5 cut(s) 89, 748, 957, 1457, 2415
Bst6I CTCTTC 6 cut(s) 441, 1201, 2110, 3487, 4192, 4700
BstACI GRCGYC 1 cut(s) 8
BstAPI GCANNNNNTGC 2 cut(s) 1692, 4601
BstAUI TGTACA 1 cut(s) 4533
BstDSI CCRYGG 3 cut(s) 234, 2647, 4353
BstEII GGTNACC 1 cut(s) 3770
BstENI CCTNNNNNAGG 2 cut(s) 1036, 3719
BstFNI CGCG 3 cut(s) 220, 348, 713
BstH2I RGCGCY 1 cut(s) 931
BstHHI GCGC 5 cut(s) 348, 403, 713, 930, 1667
BstNI CCWGG 5 cut(s) 89, 748, 957, 1457, 2415
BstNSI RCATGY 5 cut(s) 625, 1497, 3016, 3287, 4294
BstPAI GACNNNNGTC 1 cut(s) 1217
BstPI GGTNACC 1 cut(s) 3770
BstSCI CCNGG 5 cut(s) 87, 746, 955, 1455, 2413
BstSFI CTRYAG 4 cut(s) 3372, 3877, 3956, 4439
BstSLI GKGCMC 2 cut(s) 462, 2005
BstUI CGCG 3 cut(s) 220, 348, 713
BstV2I GAAGAC 7 cut(s) 684, 2148, 3139, 3485, 3764, 4452, 4901
BstX2I RGATCY 9 cut(s) 688, 2780, 3376, 3434, 3564, 3634, 3687, 4047, 5073
BstXI CCANNNNNNTGG 2 cut(s) 985, 4693
BstYI RGATCY 9 cut(s) 688, 2780, 3376, 3434, 3564, 3634, 3687, 4047, 5073
BstZ17I GTATAC 1 cut(s) 3683
Bsu15I ATCGAT 4 cut(s) 193, 3843, 4989, 4996
Bsu36I CCTNAGG 2 cut(s) 2985, 3630
BsuRI GGCC 6 cut(s) 92, 302, 2425, 3835, 4502, 4884
BsuTUI ATCGAT 4 cut(s) 193, 3843, 4989, 4996
BtgI CCRYGG 3 cut(s) 234, 2647, 4353
BtgZI GCGATG 2 cut(s) 2271, 2415
BtsI GCAGTG 3 cut(s) 460, 4448, 4646
BveI ACCTGC 2 cut(s) 3650, 4978
CaiI CAGNNNCTG 1 cut(s) 4568
CciI TCATGA 1 cut(s) 3541
CfoI GCGC 5 cut(s) 348, 403, 713, 930, 1667
Cfr10I RCCGGY 3 cut(s) 3774, 4366, 4884
Cfr13I GGNCC 5 cut(s) 1581, 1843, 3461, 3833, 4679
ClaI ATCGAT 4 cut(s) 193, 3843, 4989, 4996
CseI GACGC 2 cut(s) 16, 3083
Csp6I GTAC 6 cut(s) 1506, 3414, 4364, 4483, 4534, 4772
CspAI ACCGGT 2 cut(s) 3774, 4366
CviQI GTAC 6 cut(s) 1506, 3414, 4364, 4483, 4534, 4772
DraI TTTAAA 3 cut(s) 3364, 3819, 4326
DraIII CACNNNGTG 1 cut(s) 1910
DrdI GACNNNNNNGTC 1 cut(s) 1883
DseDI GACNNNNNNGTC 1 cut(s) 1883
EaeI YGGCCR 1 cut(s) 4882
Eam1104I CTCTTC 6 cut(s) 441, 1201, 2110, 3487, 4192, 4700
EarI CTCTTC 6 cut(s) 441, 1201, 2110, 3487, 4192, 4700
EciI GGCGGA 2 cut(s) 599, 4726
Ecl136II GAGCTC 5 cut(s) 148, 1194, 2194, 3027, 3889
Eco130I CCWWGG 6 cut(s) 692, 2197, 2758, 3421, 4622, 4723
Eco24I GRGCYC 7 cut(s) 150, 244, 453, 1196, 2196, 3029, 3891
Eco31I GGTCTC 2 cut(s) 2775, 3783
Eco32I GATATC 2 cut(s) 428, 2233
Eco47I GGWCC 4 cut(s) 1581, 1843, 3461, 4679
Eco53kI GAGCTC 5 cut(s) 148, 1194, 2194, 3027, 3889
Eco57I CTGAAG 7 cut(s) 2749, 2972, 3125, 3133, 3187, 3581, 4418
Eco81I CCTNAGG 2 cut(s) 2985, 3630
Eco88I CYCGRG 2 cut(s) 3464, 3651
Eco91I GGTNACC 1 cut(s) 3770
EcoICRI GAGCTC 5 cut(s) 148, 1194, 2194, 3027, 3889
EcoNI CCTNNNNNAGG 2 cut(s) 1036, 3719
EcoO65I GGTNACC 1 cut(s) 3770
EcoRI GAATTC 4 cut(s) 3584, 4849, 5024, 5101
EcoRII CCWGG 5 cut(s) 87, 746, 955, 1455, 2413
EcoRV GATATC 2 cut(s) 428, 2233
EcoT14I CCWWGG 6 cut(s) 692, 2197, 2758, 3421, 4622, 4723
EcoT22I ATGCAT 9 cut(s) 970, 1114, 1499, 2337, 3014, 3137, 3289, 3664, 4110
EcoT38I GRGCYC 7 cut(s) 150, 244, 453, 1196, 2196, 3029, 3891
ErhI CCWWGG 6 cut(s) 692, 2197, 2758, 3421, 4622, 4723
Esp3I CGTCTC 2 cut(s) 1395, 1814
FalI AAGNNNNNCTT 4 cut(s) 1693, 1725, 4575, 4607
FaqI GGGAC 3 cut(s) 1247, 3447, 4853
FauI CCCGC 2 cut(s) 213, 4997
FauNDI CATATG 2 cut(s) 1414, 3488
FbaI TGATCA 7 cut(s) 900, 1897, 2509, 3169, 3427, 4162, 4279
FblI GTMKAC 2 cut(s) 3682, 4734
FriOI GRGCYC 7 cut(s) 150, 244, 453, 1196, 2196, 3029, 3891
FspI TGCGCA 1 cut(s) 402
GlaI GCGC 5 cut(s) 347, 402, 712, 929, 1666
GsaI CCCAGC 1 cut(s) 2256
GsuI CTGGAG 3 cut(s) 137, 978, 1131
HaeII RGCGCY 1 cut(s) 931
HaeIII GGCC 6 cut(s) 92, 302, 2425, 3835, 4502, 4884
HapII CCGG 9 cut(s) 485, 629, 857, 2157, 2774, 3719, 3775, 4367, 4885
HgaI GACGC 2 cut(s) 16, 3083
HhaI GCGC 5 cut(s) 348, 403, 713, 930, 1667
Hin1I GRCGYC 1 cut(s) 8
Hin6I GCGC 5 cut(s) 346, 401, 711, 928, 1665
HinP1I GCGC 5 cut(s) 346, 401, 711, 928, 1665
HincII GTYRAC 1 cut(s) 4140
HindII GTYRAC 1 cut(s) 4140
HindIII AAGCTT 2 cut(s) 1372, 4495
HpaI GTTAAC 1 cut(s) 4140
HpaII CCGG 9 cut(s) 485, 629, 857, 2157, 2774, 3719, 3775, 4367, 4885
HphI GGTGA 8 cut(s) 77, 276, 910, 1000, 2029, 3437, 4088, 4369
Hpy166II GTNNAC 7 cut(s) 133, 460, 1474, 2003, 3683, 4140, 4735
Hpy8I GTNNAC 7 cut(s) 133, 460, 1474, 2003, 3683, 4140, 4735
Hpy99I CGWCG 1 cut(s) 10
HpyCH4IV ACGT 5 cut(s) 1823, 2671, 3258, 4413, 4737
HpySE526I ACGT 5 cut(s) 1823, 2671, 3258, 4413, 4737
Hsp92I GRCGYC 1 cut(s) 8
HspAI GCGC 5 cut(s) 346, 401, 711, 928, 1665
Kpn2I TCCGGA 3 cut(s) 484, 2773, 3718
KpnI GGTACC 1 cut(s) 4486
KroI GCCGGC 1 cut(s) 4884
KroNI GCCGGC 1 cut(s) 4886
Ksp22I TGATCA 7 cut(s) 900, 1897, 2509, 3169, 3427, 4162, 4279
KspAI GTTAAC 1 cut(s) 4140
LguI GCTCTTC 2 cut(s) 441, 1201
MaeII ACGT 5 cut(s) 1823, 2671, 3258, 4413, 4737
MbiI CCGCTC 2 cut(s) 1285, 1618
MfeI CAATTG 2 cut(s) 918, 3897
MflI RGATCY 9 cut(s) 688, 2780, 3376, 3434, 3564, 3634, 3687, 4047, 5073
MlyI GAGTC 7 cut(s) 969, 1235, 2093, 2577, 3606, 3754, 4013
MmeI TCCRAC 3 cut(s) 295, 2772, 3662
Mph1103I ATGCAT 9 cut(s) 970, 1114, 1499, 2337, 3014, 3137, 3289, 3664, 4110
MroI TCCGGA 3 cut(s) 484, 2773, 3718
MroNI GCCGGC 1 cut(s) 4884
MroXI GAANNNNTTC 3 cut(s) 1047, 3196, 3739
MslI CAYNNNNRTG 5 cut(s) 465, 983, 2523, 4691, 4868
MspA1I CMGCKG 1 cut(s) 4438
MspI CCGG 9 cut(s) 485, 629, 857, 2157, 2774, 3719, 3775, 4367, 4885
MspR9I CCNGG 5 cut(s) 89, 748, 957, 1457, 2415
MunI CAATTG 2 cut(s) 918, 3897
Mva1269I GAATGC 9 cut(s) 163, 821, 968, 1114, 1931, 2849, 3135, 3450, 5012
MvaI CCWGG 5 cut(s) 89, 748, 957, 1457, 2415
MvnI CGCG 3 cut(s) 220, 348, 713
NaeI GCCGGC 1 cut(s) 4886
NdeI CATATG 2 cut(s) 1414, 3488
NgoMIV GCCGGC 1 cut(s) 4884
NheI GCTAGC 1 cut(s) 2342
NmeAIII GCCGAG 1 cut(s) 36
NmuCI GTSAC 4 cut(s) 988, 1874, 1902, 2955
NsbI TGCGCA 1 cut(s) 402
NsiI ATGCAT 9 cut(s) 970, 1114, 1499, 2337, 3014, 3137, 3289, 3664, 4110
NspI RCATGY 5 cut(s) 625, 1497, 3016, 3287, 4294
PaeI GCATGC 2 cut(s) 625, 3287
PagI TCATGA 1 cut(s) 3541
PciSI GCTCTTC 2 cut(s) 441, 1201
PcsI WCGNNNNNNNCGW 2 cut(s) 1437, 4350
PctI GAATGC 9 cut(s) 163, 821, 968, 1114, 1931, 2849, 3135, 3450, 5012
PdiI GCCGGC 1 cut(s) 4886
PdmI GAANNNNTTC 3 cut(s) 1047, 3196, 3739
PflFI GACNNNGTC 2 cut(s) 1847, 3596
PflMI CCANNNNNTGG 1 cut(s) 2072
PfoI TCCNGGA 1 cut(s) 955
PinAI ACCGGT 2 cut(s) 3774, 4366
PleI GAGTC 7 cut(s) 968, 1234, 2093, 2576, 3605, 3754, 4012
PpsI GAGTC 7 cut(s) 968, 1234, 2093, 2576, 3605, 3754, 4012
PshAI GACNNNNGTC 1 cut(s) 1217
PsiI TTATAA 3 cut(s) 1007, 1299, 1520
Psp124BI GAGCTC 5 cut(s) 150, 1196, 2196, 3029, 3891
Psp6I CCWGG 5 cut(s) 87, 746, 955, 1455, 2413
PspEI GGTNACC 1 cut(s) 3770
PspFI CCCAGC 1 cut(s) 2252
PspGI CCWGG 5 cut(s) 87, 746, 955, 1455, 2413
PspPI GGNCC 5 cut(s) 1581, 1843, 3461, 3833, 4679
PstI CTGCAG 2 cut(s) 3376, 4443
PstNI CAGNNNCTG 1 cut(s) 4568
PsuI RGATCY 9 cut(s) 688, 2780, 3376, 3434, 3564, 3634, 3687, 4047, 5073
PsyI GACNNNGTC 2 cut(s) 1847, 3596
PvuII CAGCTG 1 cut(s) 4438
RsaI GTAC 6 cut(s) 1507, 3415, 4365, 4484, 4535, 4773
RsaNI GTAC 6 cut(s) 1506, 3414, 4364, 4483, 4534, 4772
RseI CAYNNNNRTG 5 cut(s) 465, 983, 2523, 4691, 4868
SacI GAGCTC 5 cut(s) 150, 1196, 2196, 3029, 3891
SapI GCTCTTC 2 cut(s) 441, 1201
Sau96I GGNCC 5 cut(s) 1581, 1843, 3461, 3833, 4679
SchI GAGTC 7 cut(s) 969, 1235, 2093, 2577, 3606, 3754, 4013
ScrFI CCNGG 5 cut(s) 89, 748, 957, 1457, 2415
SfcI CTRYAG 4 cut(s) 3372, 3877, 3956, 4439
SinI GGWCC 4 cut(s) 1581, 1843, 3461, 4679
SmiI ATTTAAAT 1 cut(s) 3364
SmiMI CAYNNNNRTG 5 cut(s) 465, 983, 2523, 4691, 4868
SmlI CTYRAG 5 cut(s) 200, 2447, 2891, 3744, 3997
SmoI CTYRAG 5 cut(s) 200, 2447, 2891, 3744, 3997
SpeI ACTAGT 1 cut(s) 1163
SphI GCATGC 2 cut(s) 625, 3287
SspI AATATT 3 cut(s) 1342, 3195, 4473
SstI GAGCTC 5 cut(s) 150, 1196, 2196, 3029, 3891
StyD4I CCNGG 5 cut(s) 87, 746, 955, 1455, 2413
StyI CCWWGG 6 cut(s) 692, 2197, 2758, 3421, 4622, 4723
SwaI ATTTAAAT 1 cut(s) 3364
TaiI ACGT 5 cut(s) 1826, 2674, 3261, 4416, 4740
TaqII GACCGA 1 cut(s) 2111
TatI WGTACW 1 cut(s) 4533
TauI GCSGC 3 cut(s) 351, 664, 822
TseFI GTSAC 4 cut(s) 988, 1874, 1902, 2955
Tsp45I GTSAC 4 cut(s) 988, 1874, 1902, 2955
TspGWI ACGGA 2 cut(s) 3609, 4346
Tth111I GACNNNGTC 2 cut(s) 1847, 3596
Van91I CCANNNNNTGG 1 cut(s) 2072
VneI GTGCAC 2 cut(s) 458, 2001
VpaK11BI GGWCC 4 cut(s) 1581, 1843, 3461, 4679
XagI CCTNNNNNAGG 2 cut(s) 1036, 3719
XceI RCATGY 5 cut(s) 625, 1497, 3016, 3287, 4294
XcmI CCANNNNNNNNNTGG 2 cut(s) 754, 1853
XmiI GTMKAC 2 cut(s) 3682, 4734
XmnI GAANNNNTTC 3 cut(s) 1047, 3196, 3739
Zsp2I ATGCAT 9 cut(s) 970, 1114, 1499, 2337, 3014, 3137, 3289, 3664, 4110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.