MD06G1134600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000526
Physical Location & Seq
Forward (+)
27894872 .. 27901983
7112 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1134600.v1.1.491

Sequence Viewer

Length: 5127 bp
ATGGCTACTCCGAAAGCGCACGTGGAAGAGATAAGGAAGACAAAGTTCTCCATTGGAAGGGAAGAGGCAAACCCTTTGACCGAGGATCTTCACCAGGCTGTTAAGAATCTCTCTGCTGAACTCTACGCTAAGGATGTTCACTTCCTCATGGAACTCATCCAGAATGCAGAAGATAATGATTACCCAGAGGGGGTTGATCCATCACTTGAGTTTGTCATAACTTCCCGGGATATAACAGCCGCGGGGGCTCCTGCTACTTTAATTGTTTTCAACAATGAGAAGGGGTTTTCACCAAAGAACATCGAGTCCATTTGCAGTGTTGGGCGTTCCACCAAGAAAGGCAACAGGAAGCGCGGCTACATTGGGGAGAAAGGTATTGGGTTCAAAAGCGTGTTTCTCATTACTGCACGACCTTACATTTTCAGTAACGGATATCAAATAAGGTTCAGTGAAGAGCCCTGTGTGCACTGCAATGTTGGCTACATAGTCCCAGAATGGGTTGATGCAAACCCTACTCTTTCAGAAATAAAACAGATATATGGTTCTGGTTCTGGTTCTGCCCTTCCAACCACAACACTGATCTTACCTTTGAAGGCGGACAAGGTTGAAGCCGTTAAGCAACAACTCTCTAGCATGCATCCGGAAATTCTCTTGTTCTTATCAAAGATTAAGCGACTCTCGGTTAGGGAAGACAATGTGAATCCAAGGCACAACACTGTAAGTGCGATAGCAATATCAAGCGAGACTGATTTTGTCACCAGGAAGAACATAGATGCTGAATCCTACACACTCCACCTTTCTGCAAAAGAAAATGGCTACAAATTTGATACAGAATGCGGCTATTCTATGTGGAAGCAGAAGTTTCCTGTCAGCCGGGAGTGCAGAGTGGATAAGAGAAATGATGTAGATGAATGGGTGATCACACTTGCATTTCCAATTGGAGAGCGCCTTTGTAGAGGAACAAACACATCTCCTGGAGTCTATGCATTCCTTCCCACTGAGATGGTGACAAACTTTCCCTTTATAATTCAAGCTGATTTTCTTCTTGCCTCATCAAGGGAAACAATTCTTCTGGACAGCCAATGGAATAAGGGGATTCTCGATTGCGTTCCAACTGTATTTGTGAATGCATTTATCTCACTTGTCAGATCTGAAGAAGATGCTCCAGTTTCTACCTTGACTCGTATGTTCAAGTTTCTGCCCGTTCAGAGCTCTCCTTATGAAGGATTGAATGTTGTCAGGGAGTCTATAAAGGCAAAACTTGTTGAAGAAAGCATTGTACCTAGTGAGCCTCACAAGGAGCAGAAGTTCTTTTATAAACCCCGTGAGGTTGGTAGGTTAATGCCTGCCTTTTGGAATATTTTGAAGAAAGCGAAGAAGCAAGGGGTAAGCTTGGTTAATCTGTCGTCTCATGGAAAATATGTTCTGGCATATGCCTTTGACGATGTGGAGTATAACGAGATACTGAATTTCTTGGGAGTTGAAGCTGTGAACAATGAATGGTATGCAACATGCATCCGGGGTACATCTAATCTTATAACTGGGGTGCCAGAAGATGTCTATTTGGAGCTTCTACTTTTTATTTCTGAATTTTGGGGTTCTAAATTTTCAAGCACCAGCATCAAGAATATACCGCTCATAAAATGTGGGGGTTATCGAAACAAATCTCTGTGCAGCATAAGTGCAATCCAGAAAGGCGAAAGCAAAGTTTGCGTATCAAGTGATTCTTCTCATATTTCATGGCTGATTGACTGGAACATGGAGTTTGCATCTGTTTCCAATGTTCTCTTTATGCCAGGGACCACACAGGAAGCTCTCAGATTGTGTTCTAGGAAGGAGACGTTGAAGAAGTGGCTTTTGGACCAAGTCAAGGTTGGTTCTGTAAGTATATATGACTATGCAGTCAATCTCTTTGATAAGTCACTTTGTGAGCGGAAGGTTGCTATTGCATTCGCCCACTTTTTACACCAGTCTTTGCGCAAGGGATATATCTTATCTTGGGAAGCTGTTAATTTGTGTGCACTAATGCCATTGGTGGATAAGTATGGCAACATCACCAGCACTAGGAAGGGAGTTCTTGTTCCAGCTAAAGGAAGCAAATGGGCAGGACTGACCGACTCTAATCTGTGGAGAAGAGAAGGCTATGTTGAGTTGAACGAAGACTATTTGGATTCCGGTCATTTTGCTGGTAGCTTTACACAACAGAAGGAGCTCCTTGGGTTTCTGAAAGATCATGCTAAAGCCTCAGATATCCCAAATGTTTGTGCTCCCAGCGATGGTATATCATCTTTATCTGCACCACTCAATAAGCAAAATGTATTCTTGCTTTTGGACTGGATTCGCCAGCTCATGCGTGAAGCTAGCATCCCTCAGAAGTTCTTGATGTGTGTGAAAGAAGGTAGCTGGCTGAAAGTTACTTTGAATGGTTCTCCTGGTTTCAGGCCACCATCGCAGTCATTTCTTCTCAAGTCATCATCGGGAAACCTTTTGCAGAATAGCTCTGTTTTTGTTGATATACCGTTGATTGATCAAAGTCATTATGATGGAAAGATTAACGATTACAAGGAGGAGCTGAAAAAAATTGGAGTCAGGTTTGAGTTTGGAGAAGCGTGTGAATATATGGGGAAGCATCTCATGTCTCTTGCTGCTGCTTCCTCTTTAACCCGTGGCAATGTATTTTCTGTACTACGTTTCATCAGATTTTTGAGAGAGAAACTTCTTCCACCAGACGATTTTATCAGCAGTATTAAAGATGGTCAGTGGCTTAAAACTTCCCTTGGCTTCAGGTCTCCGGTTGGATCTGTCCTGTCGGATGATGAGTGGACAGTTGCTTCGCAAATTAGTGACATTCCCTTCATAGATGAGGCATTCTATGGTAAAGAAGAAATCTGTCAGTTCAAAACAGAACTTGAGTTGCTTGGGGTGGCGGTCTCATTCAGCAAATGCTACCAACTGATAATTGACAACCTGAAGTCACATTCTTGCTTGACTTCCTGGACACCTGAGGTTCTTCTATTGATGCTTAAATGCATGTGTCTTTCGAGCTCATCTGAAAAACTTGTCAGTGCTTTGAAAGGATCGAATTGCCTAAAGACAAAAGCTGGTTACAAACGTCCAGAGGAATGCCTCCTGTTTGACAAGGATTGGGGCTGCATTCTTCAGGTCTTCAGTGATCTCCCATTGATTGATCACGATTTCTATGGAGACAGAATATTTTCCTTCAGAAATGAGTTGAAGAAAACTGGGGTGGTGGTTGATTTTGAGGATGCAGCAAAAGTATTTGCCCGTTATTTCAAGCAGCATGCATCTTCGACTTCCATTTCAAAAGAAAACGTTGCATCAATTCTCTTGTGTTACAGAACACTGAATGTAACTCCATTTAAATTTCCTGCAGATCTTAAGAGCTGCATTCGCGAGGTGAAGTGGCTGCGTACTCGCCTTGGTGATTATAGATCTCCCAAACAGTGCATTCTCTCTGGTCCCGAGTGGGATTCCATCTCTTCCATATGTCTTCTCCCATTCATTGATGATTCTGAAAATCATTATGGAAAGAACATTCATGCATTTAAGAACGAACTGAAGAGCTTGGGGGTGGTTGTGGAATTCAAGGACGGAGTCAAGTTTGTGGAATCTTGTCTGTATCTTCCCCAGGATCCGACCTGCATTTCTCGAGAAAATGCATTAGCATTGCTTGAATGTATACAGATCTTATTGCAGGAGAAAGCCTACTCCCTTCCGGAGGCTTTCAGGGGAAAACTTTCTCAAGCATGGTTGAAGACTCATGCTGGTTACCGGTCTCCTAACCAGTGCTTGTTGTTTGATTCGGAAACACATTTAAAGCATAATGATGGGCCTTTCATCGATGAAGAATTTTATGGCTCTAAAATTACAACCTACAGAAAGGAGCTCGCTGCAATTGGAGTGATTGTCGAAGTGAAAAAGGGATGTGCCTTGATTGCTAGGCACCTTGATCTACAGGATGAGTTTTCGACATTTGTTCGAGTATATAGTTACTTGAGTGAGTCTAAGTGGGTTCCACTGACGGATGGAGAGGCTCCCCAAAGGATCTGGGTTCCGAAAGAAAATAAAAATGGTGAGTGGGTTAGTGCAGATGAATGTGTTATGCATGACAAGGATGGTTTGTTTGGTTCGCAGTTAACTGTTTTGGAGAAACACTATGATCAAAAGTTGTTTGGTTTCTTCTCTTCTGCTTTTGGAATAAGATCCCATCCTTCTGTTGATGATTACTTGAATCTCTGGAAAGTTTGGGAAAGTTCTGAAAGCGAATTGTTGCATGATCAATGCTGCATGTTCTGGCTTTATGTTTCGAAGCACTGGAATTTAAAAACGGAGAAAAGTCTTGCTGATGCCGTGGTGAAGGTACCGGTTTATTCTCGCTCAGGTAAAATCTTGTTGTGCAACAAGGAGTACGTTTTCATTGCTGATGACCTTCAGCTGCAGTGTCTTTTTGAACAGTCATCACAGTCAATATTTGTTTGGTACCCTCAGCCAGGCTTGGCCTCCTTGCCTCGAACTAAGCTGCTTGAAATGTACAACAAAATTGGTGTTCGCACTATTTCAGAATCTGTGCAGAAGGAAGAAGTATCTCTTGCAAATGATGCTGAATTACAAATAATCCCAAGGGAAAGATTGTTCGGAAAAGCACTGCTGAGGCTGATTCTTGGATTTCTTGCTGGTCCTCCCATTGAAATGGAAGTGGAGAAGAGGCGGAAAGCTGTCCGAGGTCTTGTAGACGTTGCTGTATTGGAGACACCAGAACCAATCGCTGTACGCTATGATCTGCCACTATCTTCTGGTGAAGTTTTGAATGTGAGAGGCACACGAAAGATACGGTGGGACAGGAAGAATTCCAAATGCTTCATCCAGATGGACAAATCTGGCCGGCAGAAGAAGACCGTTGAGTTTGCTACATATTTCGCCGAAGCAATATCTGAGTGTGTTCTGTGGGAGTTCACTGAACTTATACCTGCACTTTCGGAGCTGATCAGGTTGGCATTCGTGCTAGAATTCAATGAGGAAGATGTTGATTTCTTGATGAAGTCGAAGAATTTGCAGATCTTCGTTGAGGATGTCGAGTTTCTGAATTCTGCCTGCCCTTCTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1709

Amino Acids

193.25

Weight (kDa)

6.38

Isoelectric Point (pI)

44.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 28 - 144 1.6e-11 Sacsin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28020 AT3G48770 AT3G48770
fragaria_vesca FvH4_5g09020 FvH4_5g09020
malus_domestica MD06G1092400.v1.1 MD06G1134600.v1.1 MD06G1134700.v1.1 MD06G1134800.v1.1 MD06G1135200.v1.1 MD06G1135400.v1.1 MD06G1135500.v1.1 MD08G1137600.v1.1 MD15G1395100.v1.1
prunus_persica Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145600_v2.0.a1 Prupe.5G145800_v2.0.a1 Prupe.5G145900_v2.0.a1
pyrus_communis pycom06g12550 pycom06g12570 pycom06g12580 pycom06g12590 pycom06g12610 pycom06g12630 pycom06g12640 pycom06g12650 pycom08g06280
rosa_chinensis RchiOBHm_Chr6g0249321 RchiOBHm_Chr6g0275111 RchiOBHm_Chr7g0189191 RchiOBHm_Chr7g0189261
rosa_laevigata RLG00000004622 RLG00000004623 RLG00000008237 RLG00000008238 RLG00000023303 RLG00000035187
rosa_multiflora Rmu_co8294311.1_g000001 Rmu_co8389833.1_g000001 Rmu_sc0001044.1_g000004 Rmu_sc0001329.1_g000011 Rmu_sc0006012.1_g000010 Rmu_sc0007286.1_g000032 Rmu_sc0009360.1_g000016 Rmu_sc0011817.1_g000001 Rmu_sc0015050.1_g000001
rosa_roxburghii Rroxscaffold_1G00064000 Rroxscaffold_3G00265460 Rroxscaffold_3G00265580 Rroxscaffold_3G00265640 Rroxscaffold_3G00265690 Rroxscaffold_3G00265700 Rroxscaffold_7G00193620 Rroxscaffold_7G00204550 Rroxscaffold_7G00217350
rosa_rugosa Rorug01G0465500 Rorug06G0088500 Rorug06G0496700 Rorug06G0496700
rosa_samantha Rh1AG064800 Rh2BG015100 Rh2BG493500 Rh2DG017300 Rh2DG502500 Rh4BG208500 Rh5BG528500 Rh5CG552900 Rh6CG207800 Rh6DG197900 Rh6DG198000 Rh7AG101300 Rh7AG101400 Rh7AG101600 Rh7BG104100 Rh7BG104200 Rh7CG105600 Rh7CG105700 Rh7DG103200 Rh7DG103400
rosa_wichuraiana Rw1G007300 Rw5G034670 Rw7G008750 Rw7G008780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1025, 1317, 1538
AasI GACNNNNNNGTC 1 cut(s) 1901
Acc16I TGCGCA 1 cut(s) 1979
Acc36I ACCTGC 2 cut(s) 3668, 4996
Acc65I GGTACC 2 cut(s) 4381, 4500
AccB1I GGYRCC 4 cut(s) 1546, 3963, 4381, 4500
AccBSI CCGCTC 2 cut(s) 1636, 1933
AccI GTMKAC 2 cut(s) 3700, 4752
AccII CGCG 3 cut(s) 242, 354, 3414
AccIII TCCGGA 2 cut(s) 640, 3736
AciI CCGC 9 cut(s) 240, 242, 354, 596, 837, 1634, 1933, 2927, 4729
AclI AACGTT 1 cut(s) 3333
AclWI GGATC 8 cut(s) 93, 191, 2806, 3085, 3647, 3660, 4073, 4218
AcoI YGGCCR 1 cut(s) 4900
AcuI CTGAAG 8 cut(s) 1173, 2767, 2990, 3143, 3151, 3205, 3599, 4436
AcvI CACGTG 1 cut(s) 22
AdeI CACNNNGTG 1 cut(s) 1928
AfaI GTAC 9 cut(s) 1281, 1525, 2685, 3433, 4383, 4430, 4502, 4553, 4791
AflII CTTAAG 1 cut(s) 3398
AgeI ACCGGT 2 cut(s) 3792, 4384
AjnI CCWGG 8 cut(s) 93, 758, 973, 1795, 2431, 2993, 3648, 4510
AjuI GAANNNNNNNTTGG 2 cut(s) 1841, 1873
Alw21I GWGCWC 7 cut(s) 468, 1214, 2023, 2214, 2269, 3047, 3909
Alw26I GTCTC 9 cut(s) 737, 1413, 1832, 2643, 2793, 2935, 3198, 3801, 4763
Alw44I GTGCAC 2 cut(s) 464, 2019
AlwI GGATC 8 cut(s) 93, 191, 2806, 3085, 3647, 3660, 4073, 4218
AlwNI CAGNNNCTG 1 cut(s) 4586
Ama87I CYCGRG 3 cut(s) 225, 3482, 3669
Aor13HI TCCGGA 2 cut(s) 640, 3736
AoxI GGCC 4 cut(s) 2441, 3851, 4518, 4900
ApaLI GTGCAC 2 cut(s) 464, 2019
ArsI GACNNNNNNTTYG 2 cut(s) 2815, 2847
AsiGI ACCGGT 2 cut(s) 3792, 4384
Asp700I GAANNNNTTC 3 cut(s) 1065, 3214, 3757
Asp718I GGTACC 2 cut(s) 4381, 4500
AspLEI GCGC 4 cut(s) 19, 354, 948, 1980
AspS9I GGNCC 5 cut(s) 1800, 1861, 3479, 3851, 4697
AsuC2I CCSGG 4 cut(s) 226, 227, 875, 1520
AsuII TTCGAA 1 cut(s) 4328
AsuNHI GCTAGC 1 cut(s) 2360
AvaI CYCGRG 3 cut(s) 225, 3482, 3669
AvaII GGWCC 4 cut(s) 1800, 1861, 3479, 4697
AxyI CCTNAGG 1 cut(s) 3003
BaeGI GKGCMC 2 cut(s) 468, 2023
BamHI GGATCC 1 cut(s) 3652
BanI GGYRCC 4 cut(s) 1546, 3963, 4381, 4500
BanII GRGCYC 6 cut(s) 250, 459, 1214, 2214, 3047, 3909
BarI GAAGNNNNNNTAC 2 cut(s) 341, 373
BbrPI CACGTG 1 cut(s) 22
BbsI GAAGAC 7 cut(s) 44, 696, 2166, 3157, 3503, 3782, 4919
Bbv12I GWGCWC 7 cut(s) 468, 1214, 2023, 2214, 2269, 3047, 3909
BbvCI CCTCAGC 2 cut(s) 4506, 4670
BceAI ACGGC 2 cut(s) 596, 4355
BcgI CGANNNNNNTGC 2 cut(s) 5053, 5087
BciT130I CCWGG 8 cut(s) 95, 760, 975, 1797, 2433, 2995, 3650, 4512
BclI TGATCA 6 cut(s) 918, 2527, 3187, 4180, 4297, 5004
BcnI CCSGG 4 cut(s) 226, 227, 875, 1520
BcoDI GTCTC 9 cut(s) 737, 1413, 1832, 2643, 2793, 2935, 3198, 3801, 4763
BfaI CTAG 8 cut(s) 630, 1284, 1830, 2064, 2361, 3960, 5024, 5125
BfmI CTRYAG 4 cut(s) 3390, 3895, 3974, 4457
BfoI RGCGCY 1 cut(s) 949
BfrI CTTAAG 1 cut(s) 3398
BfuAI ACCTGC 2 cut(s) 3668, 4996
BglI GCCNNNNNGGC 1 cut(s) 245
BglII AGATCT 5 cut(s) 1148, 3394, 3452, 3705, 5076
Bme18I GGWCC 4 cut(s) 1800, 1861, 3479, 4697
BmeT110I CYCGRG 3 cut(s) 225, 3482, 3669
BmgT120I GGNCC 5 cut(s) 1800, 1861, 3479, 3851, 4697
BmrI ACTGGG 2 cut(s) 1551, 3252
BmtI GCTAGC 1 cut(s) 2364
BmuI ACTGGG 2 cut(s) 1551, 3252
BpiI GAAGAC 7 cut(s) 44, 696, 2166, 3157, 3503, 3782, 4919
BpmI CTGGAG 2 cut(s) 996, 1149
Bpu10I CCTNAGC 4 cut(s) 129, 4399, 4506, 4670
Bpu14I TTCGAA 1 cut(s) 4328
BpuEI CTTGAG 5 cut(s) 227, 2450, 2930, 3747, 4036
BpuMI CCSGG 4 cut(s) 226, 227, 875, 1520
Bsa29I ATCGAT 1 cut(s) 3861
BsaAI YACGTR 1 cut(s) 22
BsaI GGTCTC 3 cut(s) 2793, 2935, 3801
BsaWI WCCGGW 6 cut(s) 640, 2174, 2791, 3736, 3792, 4384
BsaXI ACNNNNNCTCC 6 cut(s) 1292, 1322, 2064, 2094, 3912, 3942
Bse118I RCCGGY 3 cut(s) 3792, 4384, 4902
Bse1I ACTGG 8 cut(s) 1166, 1546, 1757, 1969, 2339, 3247, 3805, 4340
Bse21I CCTNAGG 1 cut(s) 3003
Bse3DI GCAATG 4 cut(s) 478, 2677, 3686, 4437
BseAI TCCGGA 2 cut(s) 640, 3736
BseBI CCWGG 8 cut(s) 95, 760, 975, 1797, 2433, 2995, 3650, 4512
BseCI ATCGAT 1 cut(s) 3861
BseMI GCAATG 4 cut(s) 478, 2677, 3686, 4437
BseMII CTCAG 9 cut(s) 990, 1831, 2259, 2384, 2994, 4413, 4520, 4661, 4944
BseNI ACTGG 8 cut(s) 1166, 1546, 1757, 1969, 2339, 3247, 3805, 4340
BseRI GAGGAG 1 cut(s) 2582
BseSI GKGCMC 2 cut(s) 468, 2023
BseYI CCCAGC 1 cut(s) 2270
BsgI GTGCAG 7 cut(s) 390, 901, 1693, 2280, 4128, 4610, 4974
Bsh1236I CGCG 3 cut(s) 242, 354, 3414
BshFI GGCC 4 cut(s) 2443, 3853, 4520, 4902
BshNI GGYRCC 4 cut(s) 1546, 3963, 4381, 4500
BshTI ACCGGT 2 cut(s) 3792, 4384
BshVI ATCGAT 1 cut(s) 3861
BsiHKAI GWGCWC 7 cut(s) 468, 1214, 2023, 2214, 2269, 3047, 3909
BsiHKCI CYCGRG 3 cut(s) 225, 3482, 3669
BslFI GGGAC 4 cut(s) 473, 1813, 3465, 4871
BsmAI GTCTC 9 cut(s) 737, 1413, 1832, 2643, 2793, 2935, 3198, 3801, 4763
BsmBI CGTCTC 2 cut(s) 1413, 1832
BsmFI GGGAC 4 cut(s) 473, 1813, 3465, 4871
BsnI GGCC 4 cut(s) 2443, 3853, 4520, 4902
Bso31I GGTCTC 3 cut(s) 2793, 2935, 3801
BsoBI CYCGRG 3 cut(s) 225, 3482, 3669
Bsp119I TTCGAA 1 cut(s) 4328
Bsp1286I GDGCHC 9 cut(s) 250, 459, 468, 1214, 2023, 2214, 2269, 3047, 3909
Bsp13I TCCGGA 2 cut(s) 640, 3736
Bsp1407I TGTACA 1 cut(s) 4551
Bsp68I TCGCGA 1 cut(s) 3414
BspACI CCGC 9 cut(s) 240, 242, 354, 596, 837, 1634, 1933, 2927, 4729
BspANI GGCC 4 cut(s) 2443, 3853, 4520, 4902
BspCNI CTCAG 9 cut(s) 991, 1830, 2258, 2383, 2995, 4412, 4519, 4662, 4945
BspDI ATCGAT 1 cut(s) 3861
BspEI TCCGGA 2 cut(s) 640, 3736
BspFNI CGCG 3 cut(s) 242, 354, 3414
BspMAI CTGCAG 2 cut(s) 3394, 4461
BspMI ACCTGC 2 cut(s) 3668, 4996
BspOI GCTAGC 1 cut(s) 2364
BspPI GGATC 8 cut(s) 93, 191, 2806, 3085, 3647, 3660, 4073, 4218
BspQI GCTCTTC 2 cut(s) 447, 3575
BspT104I TTCGAA 1 cut(s) 4328
BspT107I GGYRCC 4 cut(s) 1546, 3963, 4381, 4500
BspTI CTTAAG 1 cut(s) 3398
BspTNI GGTCTC 3 cut(s) 2793, 2935, 3801
BsrBI CCGCTC 2 cut(s) 1636, 1933
BsrDI GCAATG 4 cut(s) 478, 2677, 3686, 4437
BsrFI RCCGGY 3 cut(s) 3792, 4384, 4902
BsrGI TGTACA 1 cut(s) 4551
BsrI ACTGG 8 cut(s) 1166, 1546, 1757, 1969, 2339, 3247, 3805, 4340
BssAI RCCGGY 3 cut(s) 3792, 4384, 4902
BssNAI GTATAC 1 cut(s) 3701
BssT1I CCWWGG 5 cut(s) 704, 2215, 2776, 3439, 4640
Bst1107I GTATAC 1 cut(s) 3701
Bst2UI CCWGG 8 cut(s) 95, 760, 975, 1797, 2433, 2995, 3650, 4512
Bst6I CTCTTC 8 cut(s) 21, 57, 447, 2128, 3505, 3575, 4210, 4718
BstAFI CTTAAG 1 cut(s) 3398
BstAPI GCANNNNNTGC 2 cut(s) 1710, 4619
BstAUI TGTACA 1 cut(s) 4551
BstBAI YACGTR 1 cut(s) 22
BstBI TTCGAA 1 cut(s) 4328
BstC8I GCNNGC 9 cut(s) 635, 1347, 2345, 2362, 2405, 3303, 3909, 4904, 5113
BstDSI CCRYGG 3 cut(s) 240, 2665, 4371
BstEII GGTNACC 1 cut(s) 3788
BstENI CCTNNNNNAGG 4 cut(s) 1054, 1221, 3737, 4509
BstFNI CGCG 3 cut(s) 242, 354, 3414
BstH2I RGCGCY 1 cut(s) 949
BstHHI GCGC 4 cut(s) 19, 354, 948, 1980
BstMAI GTCTC 9 cut(s) 737, 1413, 1832, 2643, 2793, 2935, 3198, 3801, 4763
BstMWI GCNNNNNNNGC 9 cut(s) 245, 463, 477, 879, 1710, 2449, 3411, 3956, 4619
BstNI CCWGG 8 cut(s) 95, 760, 975, 1797, 2433, 2995, 3650, 4512
BstNSI RCATGY 5 cut(s) 637, 1515, 3034, 3305, 4312
BstPI GGTNACC 1 cut(s) 3788
BstSFI CTRYAG 4 cut(s) 3390, 3895, 3974, 4457
BstSLI GKGCMC 2 cut(s) 468, 2023
BstUI CGCG 3 cut(s) 242, 354, 3414
BstV2I GAAGAC 7 cut(s) 44, 696, 2166, 3157, 3503, 3782, 4919
BstXI CCANNNNNNTGG 2 cut(s) 1003, 4711
BstZ17I GTATAC 1 cut(s) 3701
Bsu15I ATCGAT 1 cut(s) 3861
Bsu36I CCTNAGG 1 cut(s) 3003
BsuRI GGCC 4 cut(s) 2443, 3853, 4520, 4902
BsuTUI ATCGAT 1 cut(s) 3861
BtgI CCRYGG 3 cut(s) 240, 2665, 4371
BtgZI GCGATG 2 cut(s) 2289, 2433
BtsI GCAGTG 4 cut(s) 322, 466, 4466, 4664
BtuMI TCGCGA 1 cut(s) 3414
BveI ACCTGC 2 cut(s) 3668, 4996
Cac8I GCNNGC 9 cut(s) 635, 1347, 2345, 2362, 2405, 3303, 3909, 4904, 5113
CaiI CAGNNNCTG 1 cut(s) 4586
CfoI GCGC 4 cut(s) 19, 354, 948, 1980
Cfr10I RCCGGY 3 cut(s) 3792, 4384, 4902
Cfr13I GGNCC 5 cut(s) 1800, 1861, 3479, 3851, 4697
Cfr42I CCGCGG 1 cut(s) 243
Cfr9I CCCGGG 1 cut(s) 225
ClaI ATCGAT 1 cut(s) 3861
Csp6I GTAC 9 cut(s) 1280, 1524, 2684, 3432, 4382, 4429, 4501, 4552, 4790
CspAI ACCGGT 2 cut(s) 3792, 4384
CviQI GTAC 9 cut(s) 1280, 1524, 2684, 3432, 4382, 4429, 4501, 4552, 4790
DraI TTTAAA 3 cut(s) 3382, 3837, 4344
DraIII CACNNNGTG 1 cut(s) 1928
DrdI GACNNNNNNGTC 1 cut(s) 1901
DseDI GACNNNNNNGTC 1 cut(s) 1901
EaeI YGGCCR 1 cut(s) 4900
Eam1104I CTCTTC 8 cut(s) 21, 57, 447, 2128, 3505, 3575, 4210, 4718
EarI CTCTTC 8 cut(s) 21, 57, 447, 2128, 3505, 3575, 4210, 4718
EciI GGCGGA 2 cut(s) 611, 4744
Ecl136II GAGCTC 4 cut(s) 1212, 2212, 3045, 3907
Eco130I CCWWGG 5 cut(s) 704, 2215, 2776, 3439, 4640
Eco24I GRGCYC 6 cut(s) 250, 459, 1214, 2214, 3047, 3909
Eco31I GGTCTC 3 cut(s) 2793, 2935, 3801
Eco32I GATATC 2 cut(s) 434, 2251
Eco47I GGWCC 4 cut(s) 1800, 1861, 3479, 4697
Eco53kI GAGCTC 4 cut(s) 1212, 2212, 3045, 3907
Eco57I CTGAAG 8 cut(s) 1173, 2767, 2990, 3143, 3151, 3205, 3599, 4436
Eco72I CACGTG 1 cut(s) 22
Eco81I CCTNAGG 1 cut(s) 3003
Eco88I CYCGRG 3 cut(s) 225, 3482, 3669
Eco91I GGTNACC 1 cut(s) 3788
EcoICRI GAGCTC 4 cut(s) 1212, 2212, 3045, 3907
EcoNI CCTNNNNNAGG 4 cut(s) 1054, 1221, 3737, 4509
EcoO65I GGTNACC 1 cut(s) 3788
EcoRI GAATTC 4 cut(s) 3602, 4867, 5027, 5104
EcoRII CCWGG 8 cut(s) 93, 758, 973, 1795, 2431, 2993, 3648, 4510
EcoRV GATATC 2 cut(s) 434, 2251
EcoT14I CCWWGG 5 cut(s) 704, 2215, 2776, 3439, 4640
EcoT22I ATGCAT 9 cut(s) 639, 988, 1132, 1517, 3032, 3307, 3565, 3682, 4128
EcoT38I GRGCYC 6 cut(s) 250, 459, 1214, 2214, 3047, 3909
ErhI CCWWGG 5 cut(s) 704, 2215, 2776, 3439, 4640
Esp3I CGTCTC 2 cut(s) 1413, 1832
FalI AAGNNNNNCTT 4 cut(s) 1711, 1743, 4593, 4625
FaqI GGGAC 4 cut(s) 473, 1813, 3465, 4871
FauI CCCGC 1 cut(s) 235
FauNDI CATATG 2 cut(s) 1432, 3506
FbaI TGATCA 6 cut(s) 918, 2527, 3187, 4180, 4297, 5004
FblI GTMKAC 2 cut(s) 3700, 4752
FriOI GRGCYC 6 cut(s) 250, 459, 1214, 2214, 3047, 3909
FspBI CTAG 8 cut(s) 630, 1284, 1830, 2064, 2361, 3960, 5024, 5125
FspI TGCGCA 1 cut(s) 1979
GlaI GCGC 4 cut(s) 18, 353, 947, 1979
GsaI CCCAGC 1 cut(s) 2274
GsuI CTGGAG 2 cut(s) 996, 1149
HaeII RGCGCY 1 cut(s) 949
HaeIII GGCC 4 cut(s) 2443, 3853, 4520, 4902
HhaI GCGC 4 cut(s) 19, 354, 948, 1980
Hin6I GCGC 4 cut(s) 17, 352, 946, 1978
HinP1I GCGC 4 cut(s) 17, 352, 946, 1978
HincII GTYRAC 1 cut(s) 4158
HindII GTYRAC 1 cut(s) 4158
HindIII AAGCTT 1 cut(s) 1390
HpaI GTTAAC 1 cut(s) 4158
Hpy166II GTNNAC 9 cut(s) 139, 466, 1492, 2021, 2823, 3701, 4158, 4753, 4974
Hpy8I GTNNAC 9 cut(s) 139, 466, 1492, 2021, 2823, 3701, 4158, 4753, 4974
HpyCH4IV ACGT 7 cut(s) 21, 1841, 2689, 3112, 3333, 4431, 4755
HpyF10VI GCNNNNNNNGC 9 cut(s) 245, 463, 477, 879, 1710, 2449, 3411, 3956, 4619
HpySE526I ACGT 7 cut(s) 21, 1841, 2689, 3112, 3333, 4431, 4755
HspAI GCGC 4 cut(s) 17, 352, 946, 1978
Kpn2I TCCGGA 2 cut(s) 640, 3736
KpnI GGTACC 2 cut(s) 4385, 4504
KroI GCCGGC 1 cut(s) 4902
KroNI GCCGGC 1 cut(s) 4904
Ksp22I TGATCA 6 cut(s) 918, 2527, 3187, 4180, 4297, 5004
KspAI GTTAAC 1 cut(s) 4158
KspI CCGCGG 1 cut(s) 243
LguI GCTCTTC 2 cut(s) 447, 3575
MaeI CTAG 8 cut(s) 630, 1284, 1830, 2064, 2361, 3960, 5024, 5125
MaeII ACGT 7 cut(s) 21, 1841, 2689, 3112, 3333, 4431, 4755
MbiI CCGCTC 2 cut(s) 1636, 1933
MfeI CAATTG 2 cut(s) 936, 3915
MhlI GDGCHC 9 cut(s) 250, 459, 468, 1214, 2023, 2214, 2269, 3047, 3909
MmeI TCCRAC 5 cut(s) 590, 1136, 2776, 2790, 3680
Mph1103I ATGCAT 9 cut(s) 639, 988, 1132, 1517, 3032, 3307, 3565, 3682, 4128
MroI TCCGGA 2 cut(s) 640, 3736
MroNI GCCGGC 1 cut(s) 4902
MroXI GAANNNNTTC 3 cut(s) 1065, 3214, 3757
MslI CAYNNNNRTG 6 cut(s) 471, 1001, 2541, 3846, 4709, 4886
MspA1I CMGCKG 2 cut(s) 242, 4456
MspCI CTTAAG 1 cut(s) 3398
MunI CAATTG 2 cut(s) 936, 3915
MvaI CCWGG 8 cut(s) 95, 760, 975, 1797, 2433, 2995, 3650, 4512
MvnI CGCG 3 cut(s) 242, 354, 3414
MwoI GCNNNNNNNGC 9 cut(s) 245, 463, 477, 879, 1710, 2449, 3411, 3956, 4619
NaeI GCCGGC 1 cut(s) 4904
NciI CCSGG 4 cut(s) 226, 227, 875, 1520
NdeI CATATG 2 cut(s) 1432, 3506
NgoMIV GCCGGC 1 cut(s) 4902
NheI GCTAGC 1 cut(s) 2360
NmuCI GTSAC 5 cut(s) 754, 1006, 1920, 2843, 2973
NruI TCGCGA 1 cut(s) 3414
NsbI TGCGCA 1 cut(s) 1979
NsiI ATGCAT 9 cut(s) 639, 988, 1132, 1517, 3032, 3307, 3565, 3682, 4128
NspI RCATGY 5 cut(s) 637, 1515, 3034, 3305, 4312
NspV TTCGAA 1 cut(s) 4328
PaeI GCATGC 2 cut(s) 637, 3305
PaeR7I CTCGAG 1 cut(s) 3669
PciSI GCTCTTC 2 cut(s) 447, 3575
PdiI GCCGGC 1 cut(s) 4904
PdmI GAANNNNTTC 3 cut(s) 1065, 3214, 3757
PflFI GACNNNGTC 2 cut(s) 1865, 3614
PfoI TCCNGGA 2 cut(s) 973, 2993
PinAI ACCGGT 2 cut(s) 3792, 4384
PmaCI CACGTG 1 cut(s) 22
PmlI CACGTG 1 cut(s) 22
Ppu21I YACGTR 1 cut(s) 22
PsiI TTATAA 3 cut(s) 1025, 1317, 1538
Psp124BI GAGCTC 4 cut(s) 1214, 2214, 3047, 3909
Psp1406I AACGTT 1 cut(s) 3333
Psp6I CCWGG 8 cut(s) 93, 758, 973, 1795, 2431, 2993, 3648, 4510
PspCI CACGTG 1 cut(s) 22
PspEI GGTNACC 1 cut(s) 3788
PspFI CCCAGC 1 cut(s) 2270
PspGI CCWGG 8 cut(s) 93, 758, 973, 1795, 2431, 2993, 3648, 4510
PspPI GGNCC 5 cut(s) 1800, 1861, 3479, 3851, 4697
PstI CTGCAG 2 cut(s) 3394, 4461
PstNI CAGNNNCTG 1 cut(s) 4586
PsyI GACNNNGTC 2 cut(s) 1865, 3614
PvuII CAGCTG 1 cut(s) 4456
RruI TCGCGA 1 cut(s) 3414
RsaI GTAC 9 cut(s) 1281, 1525, 2685, 3433, 4383, 4430, 4502, 4553, 4791
RsaNI GTAC 9 cut(s) 1280, 1524, 2684, 3432, 4382, 4429, 4501, 4552, 4790
RseI CAYNNNNRTG 6 cut(s) 471, 1001, 2541, 3846, 4709, 4886
SacI GAGCTC 4 cut(s) 1214, 2214, 3047, 3909
SacII CCGCGG 1 cut(s) 243
SapI GCTCTTC 2 cut(s) 447, 3575
Sau96I GGNCC 5 cut(s) 1800, 1861, 3479, 3851, 4697
SduI GDGCHC 9 cut(s) 250, 459, 468, 1214, 2023, 2214, 2269, 3047, 3909
SfcI CTRYAG 4 cut(s) 3390, 3895, 3974, 4457
Sfr274I CTCGAG 1 cut(s) 3669
Sfr303I CCGCGG 1 cut(s) 243
SfuI TTCGAA 1 cut(s) 4328
SgrBI CCGCGG 1 cut(s) 243
SinI GGWCC 4 cut(s) 1800, 1861, 3479, 4697
SlaI CTCGAG 1 cut(s) 3669
SmaI CCCGGG 1 cut(s) 227
SmiI ATTTAAAT 1 cut(s) 3382
SmiMI CAYNNNNRTG 6 cut(s) 471, 1001, 2541, 3846, 4709, 4886
SmlI CTYRAG 7 cut(s) 206, 2465, 2909, 3398, 3669, 3762, 4015
SmoI CTYRAG 7 cut(s) 206, 2465, 2909, 3398, 3669, 3762, 4015
SphI GCATGC 2 cut(s) 637, 3305
SsiI CCGC 9 cut(s) 240, 242, 354, 596, 837, 1634, 1933, 2927, 4729
SspI AATATT 3 cut(s) 1360, 3213, 4491
SspMI CTAG 8 cut(s) 630, 1284, 1830, 2064, 2361, 3960, 5024, 5125
SstI GAGCTC 4 cut(s) 1214, 2214, 3047, 3909
StyI CCWWGG 5 cut(s) 704, 2215, 2776, 3439, 4640
SwaI ATTTAAAT 1 cut(s) 3382
TaiI ACGT 7 cut(s) 24, 1844, 2692, 3115, 3336, 4434, 4758
TaqII GACCGA 2 cut(s) 95, 2129
TatI WGTACW 2 cut(s) 2683, 4551
TauI GCSGC 3 cut(s) 242, 357, 840
TseFI GTSAC 5 cut(s) 754, 1006, 1920, 2843, 2973
Tsp45I GTSAC 5 cut(s) 754, 1006, 1920, 2843, 2973
TspGWI ACGGA 4 cut(s) 444, 3627, 4058, 4364
TspMI CCCGGG 1 cut(s) 225
Tth111I GACNNNGTC 2 cut(s) 1865, 3614
Vha464I CTTAAG 1 cut(s) 3398
VneI GTGCAC 2 cut(s) 464, 2019
VpaK11BI GGWCC 4 cut(s) 1800, 1861, 3479, 4697
XagI CCTNNNNNAGG 4 cut(s) 1054, 1221, 3737, 4509
XceI RCATGY 5 cut(s) 637, 1515, 3034, 3305, 4312
XcmI CCANNNNNNNNNTGG 1 cut(s) 1871
XhoI CTCGAG 1 cut(s) 3669
XmaI CCCGGG 1 cut(s) 225
XmiI GTMKAC 2 cut(s) 3700, 4752
XmnI GAANNNNTTC 3 cut(s) 1065, 3214, 3757
XspI CTAG 8 cut(s) 630, 1284, 1830, 2064, 2361, 3960, 5024, 5125
Zsp2I ATGCAT 9 cut(s) 639, 988, 1132, 1517, 3032, 3307, 3565, 3682, 4128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.