pycom08g06280

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000526
Physical Location & Seq
Forward (+)
4860727 .. 4862458
1732 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g06280.1

Sequence Viewer

Length: 693 bp
ATGGGAAGCCACACAAATGAAGAAGCTATGGCTTCTGTTAAACGTCACATAAAAGAAATTAGGACCAGAAAGTACTCGATAGGAAAGAGAGAAGCAAACCCTTTAACCCTTGACCTTCACCATGCAGTCACCAGCCTATCTGCCGAGCTTTACCAGAAGGACATCCATTTTCTCATGGAGCTCATACAGAATGCGGAAGACAATGAATACGAAGAAGGGGTTGAACCAACGCCGGAATTTGTGATGACAAAGGACATAACTGGGAGTCGAGCTCCGGCTACTCTGCTGGTTTTTAATAAAGAGGTTGGATTTTCCAGGAAGAATATGGACTCCATCTGCAGTGTAGGACGTTCTACAAAGAAGGGGAAGAGACAGCAGGGCTTTATAGGAGAAAAAGGAATTGGATTTAAGAGCGTGTTTCTTGTGAGTTCACAGCCATGGATATTTAGCAACGGGTATCGTGTCAGATTCACGGAAGAACCAAACCAATATTGTGGTATTCGGTATGTTGTTCCTGAATTTGTTACTCGAAGACCATATTTGTCAAGAATATGCAATGCGTATGGGTCAAACAAGATCTTGCCCACAACTATATTTGTCCTTCCTCTAAAGCCTGACAAGGTTGAAGCTGTGAGAGCTCAACTGTCTGATCTGCACCCAGAGATTCTCCTGTTCTTCTCCAAGATAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

231

Amino Acids

26.22

Weight (kDa)

9.26

Isoelectric Point (pI)

36.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 51 - 154 1.1e-10 Sacsin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28020 AT3G48770 AT3G48770
fragaria_vesca FvH4_5g09020 FvH4_5g09020
malus_domestica MD06G1092400.v1.1 MD06G1134600.v1.1 MD06G1134700.v1.1 MD06G1134800.v1.1 MD06G1135200.v1.1 MD06G1135400.v1.1 MD06G1135500.v1.1 MD08G1137600.v1.1 MD15G1395100.v1.1
prunus_persica Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145600_v2.0.a1 Prupe.5G145800_v2.0.a1 Prupe.5G145900_v2.0.a1
pyrus_communis pycom06g12550 pycom06g12570 pycom06g12580 pycom06g12590 pycom06g12610 pycom06g12630 pycom06g12640 pycom06g12650 pycom08g06280
rosa_chinensis RchiOBHm_Chr6g0249321 RchiOBHm_Chr6g0275111 RchiOBHm_Chr7g0189191 RchiOBHm_Chr7g0189261
rosa_laevigata RLG00000004622 RLG00000004623 RLG00000008237 RLG00000008238 RLG00000023303 RLG00000035187
rosa_multiflora Rmu_co8294311.1_g000001 Rmu_co8389833.1_g000001 Rmu_sc0001044.1_g000004 Rmu_sc0001329.1_g000011 Rmu_sc0006012.1_g000010 Rmu_sc0007286.1_g000032 Rmu_sc0009360.1_g000016 Rmu_sc0011817.1_g000001 Rmu_sc0015050.1_g000001
rosa_roxburghii Rroxscaffold_1G00064000 Rroxscaffold_3G00265460 Rroxscaffold_3G00265580 Rroxscaffold_3G00265640 Rroxscaffold_3G00265690 Rroxscaffold_3G00265700 Rroxscaffold_7G00193620 Rroxscaffold_7G00204550 Rroxscaffold_7G00217350
rosa_rugosa Rorug01G0465500 Rorug06G0088500 Rorug06G0496700 Rorug06G0496700
rosa_samantha Rh1AG064800 Rh2BG015100 Rh2BG493500 Rh2DG017300 Rh2DG502500 Rh4BG208500 Rh5BG528500 Rh5CG552900 Rh6CG207800 Rh6DG197900 Rh6DG198000 Rh7AG101300 Rh7AG101400 Rh7AG101600 Rh7BG104100 Rh7BG104200 Rh7CG105600 Rh7CG105700 Rh7DG103200 Rh7DG103400
rosa_wichuraiana Rw1G007300 Rw5G034670 Rw7G008750 Rw7G008780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 194
AcsI RAATTY 2 cut(s) 236, 518
AfaI GTAC 1 cut(s) 74
AgsI TTSAA 2 cut(s) 224, 626
AjnI CCWGG 1 cut(s) 314
AjuI GAANNNNNNNTTGG 2 cut(s) 384, 416
AluBI AGCT 6 cut(s) 26, 148, 181, 272, 629, 638
AluI AGCT 6 cut(s) 26, 148, 181, 272, 629, 638
Alw21I GWGCWC 3 cut(s) 183, 274, 640
Alw26I GTCTC 1 cut(s) 364
ApoI RAATTY 2 cut(s) 236, 518
AspS9I GGNCC 1 cut(s) 63
AsuHPI GGTGA 2 cut(s) 110, 121
AvaII GGWCC 1 cut(s) 63
BanII GRGCYC 3 cut(s) 183, 274, 640
BbsI GAAGAC 2 cut(s) 204, 538
Bbv12I GWGCWC 3 cut(s) 183, 274, 640
BccI CCATC 1 cut(s) 341
BciT130I CCWGG 1 cut(s) 316
BcoDI GTCTC 1 cut(s) 364
BfmI CTRYAG 1 cut(s) 337
BglII AGATCT 1 cut(s) 576
BmcAI AGTACT 1 cut(s) 74
Bme1390I CCNGG 1 cut(s) 316
Bme18I GGWCC 1 cut(s) 63
BmgT120I GGNCC 1 cut(s) 63
BmrFI CCNGG 1 cut(s) 316
BmrI ACTGGG 1 cut(s) 270
BmuI ACTGGG 1 cut(s) 270
BpiI GAAGAC 2 cut(s) 204, 538
BplI GAGNNNNNCTC 2 cut(s) 256, 288
BsaJI CCNNGG 1 cut(s) 437
Bse1I ACTGG 1 cut(s) 265
Bse3DI GCAATG 1 cut(s) 562
BseBI CCWGG 1 cut(s) 316
BseDI CCNNGG 1 cut(s) 437
BseGI GGATG 1 cut(s) 162
BseMI GCAATG 1 cut(s) 562
BseNI ACTGG 1 cut(s) 265
BsgI GTGCAG 1 cut(s) 638
BsiHKAI GWGCWC 3 cut(s) 183, 274, 640
BsiSI CCGG 2 cut(s) 233, 275
BsmAI GTCTC 1 cut(s) 364
BsmI GAATGC 1 cut(s) 196
Bsp1286I GDGCHC 3 cut(s) 183, 274, 640
Bsp143I GATC 2 cut(s) 576, 649
Bsp19I CCATGG 1 cut(s) 437
BspACI CCGC 1 cut(s) 194
BspMAI CTGCAG 1 cut(s) 341
BsrDI GCAATG 1 cut(s) 562
BsrI ACTGG 1 cut(s) 265
BssECI CCNNGG 1 cut(s) 437
BssMI GATC 2 cut(s) 576, 649
BssT1I CCWWGG 1 cut(s) 437
Bst2UI CCWGG 1 cut(s) 316
Bst4CI ACNGT 1 cut(s) 645
Bst6I CTCTTC 1 cut(s) 362
BstDSI CCRYGG 1 cut(s) 437
BstF5I GGATG 1 cut(s) 162
BstKTI GATC 2 cut(s) 579, 652
BstMAI GTCTC 1 cut(s) 364
BstMBI GATC 2 cut(s) 576, 649
BstMWI GCNNNNNNNGC 1 cut(s) 635
BstNI CCWGG 1 cut(s) 316
BstSCI CCNGG 1 cut(s) 314
BstSFI CTRYAG 1 cut(s) 337
BstV2I GAAGAC 2 cut(s) 204, 538
BstX2I RGATCY 1 cut(s) 576
BstXI CCANNNNNNTGG 1 cut(s) 494
BstYI RGATCY 1 cut(s) 576
BtgI CCRYGG 1 cut(s) 437
BtsCI GGATG 1 cut(s) 162
BtsI GCAGTG 1 cut(s) 346
BtsIMutI CAGTG 1 cut(s) 346
Cfr13I GGNCC 1 cut(s) 63
Csp6I GTAC 1 cut(s) 73
CviAII CATG 3 cut(s) 122, 175, 438
CviQI GTAC 1 cut(s) 73
DpnI GATC 2 cut(s) 578, 651
DpnII GATC 2 cut(s) 576, 649
Eam1104I CTCTTC 1 cut(s) 362
EarI CTCTTC 1 cut(s) 362
Ecl136II GAGCTC 3 cut(s) 181, 272, 638
Eco130I CCWWGG 1 cut(s) 437
Eco24I GRGCYC 3 cut(s) 183, 274, 640
Eco47I GGWCC 1 cut(s) 63
Eco53kI GAGCTC 3 cut(s) 181, 272, 638
EcoICRI GAGCTC 3 cut(s) 181, 272, 638
EcoRII CCWGG 1 cut(s) 314
EcoT14I CCWWGG 1 cut(s) 437
EcoT38I GRGCYC 3 cut(s) 183, 274, 640
ErhI CCWWGG 1 cut(s) 437
FaeI CATG 3 cut(s) 125, 178, 441
FatI CATG 3 cut(s) 121, 174, 437
FokI GGATG 1 cut(s) 149
FriOI GRGCYC 3 cut(s) 183, 274, 640
HapII CCGG 2 cut(s) 233, 275
Hin1II CATG 3 cut(s) 125, 178, 441
HinfI GANTC 4 cut(s) 265, 329, 468, 664
HpaII CCGG 2 cut(s) 233, 275
HphI GGTGA 2 cut(s) 110, 121
Hpy166II GTNNAC 1 cut(s) 431
Hpy188I TCNGA 2 cut(s) 467, 649
Hpy188III TCNNGA 2 cut(s) 515, 546
Hpy8I GTNNAC 1 cut(s) 431
HpyAV CCTTC 5 cut(s) 125, 151, 209, 355, 611
HpyCH4III ACNGT 1 cut(s) 645
HpyCH4IV ACGT 2 cut(s) 43, 349
HpyCH4V TGCA 4 cut(s) 125, 339, 555, 655
HpyF10VI GCNNNNNNNGC 1 cut(s) 635
HpySE526I ACGT 2 cut(s) 43, 349
Hsp92II CATG 3 cut(s) 125, 178, 441
Kzo9I GATC 2 cut(s) 576, 649
LmnI GCTCC 2 cut(s) 178, 277
MaeII ACGT 2 cut(s) 43, 349
MaeIII GTNAC 3 cut(s) 44, 127, 523
MalI GATC 2 cut(s) 578, 651
MboI GATC 2 cut(s) 576, 649
MboII GAAGA 8 cut(s) 32, 209, 224, 331, 379, 488, 543, 667
MflI RGATCY 1 cut(s) 576
MhlI GDGCHC 3 cut(s) 183, 274, 640
MluCI AATT 4 cut(s) 57, 236, 399, 518
MlyI GAGTC 2 cut(s) 274, 323
MmeI TCCRAC 1 cut(s) 286
MnlI CCTC 2 cut(s) 295, 615
MseI TTAA 4 cut(s) 39, 104, 294, 408
MslI CAYNNNNRTG 2 cut(s) 15, 436
MspI CCGG 2 cut(s) 233, 275
MspR9I CCNGG 1 cut(s) 316
Mva1269I GAATGC 1 cut(s) 196
MvaI CCWGG 1 cut(s) 316
MwoI GCNNNNNNNGC 1 cut(s) 635
NcoI CCATGG 1 cut(s) 437
NdeII GATC 2 cut(s) 576, 649
NlaIII CATG 3 cut(s) 125, 178, 441
NmeAIII GCCGAG 1 cut(s) 169
NmuCI GTSAC 2 cut(s) 44, 127
PctI GAATGC 1 cut(s) 196
PfeI GAWTC 2 cut(s) 468, 664
PfoI TCCNGGA 1 cut(s) 314
PleI GAGTC 2 cut(s) 273, 323
PpsI GAGTC 2 cut(s) 273, 323
Psp124BI GAGCTC 3 cut(s) 183, 274, 640
Psp6I CCWGG 1 cut(s) 314
PspGI CCWGG 1 cut(s) 314
PspPI GGNCC 1 cut(s) 63
PstI CTGCAG 1 cut(s) 341
PsuI RGATCY 1 cut(s) 576
RsaI GTAC 1 cut(s) 74
RsaNI GTAC 1 cut(s) 73
RseI CAYNNNNRTG 2 cut(s) 15, 436
SacI GAGCTC 3 cut(s) 183, 274, 640
SaqAI TTAA 4 cut(s) 39, 104, 294, 408
Sau3AI GATC 2 cut(s) 576, 649
Sau96I GGNCC 1 cut(s) 63
ScaI AGTACT 1 cut(s) 74
SchI GAGTC 2 cut(s) 274, 323
ScrFI CCNGG 1 cut(s) 316
SduI GDGCHC 3 cut(s) 183, 274, 640
SfcI CTRYAG 1 cut(s) 337
SinI GGWCC 1 cut(s) 63
SmiMI CAYNNNNRTG 2 cut(s) 15, 436
Sse9I AATT 4 cut(s) 57, 236, 399, 518
SsiI CCGC 1 cut(s) 194
SspI AATATT 1 cut(s) 491
SstI GAGCTC 3 cut(s) 183, 274, 640
StyD4I CCNGG 1 cut(s) 314
StyI CCWWGG 1 cut(s) 437
TaaI ACNGT 1 cut(s) 645
TaiI ACGT 2 cut(s) 46, 352
TaqI TCGA 3 cut(s) 77, 268, 529
TasI AATT 4 cut(s) 57, 236, 399, 518
TatI WGTACW 1 cut(s) 72
TfiI GAWTC 2 cut(s) 468, 664
Tru1I TTAA 4 cut(s) 39, 104, 294, 408
Tru9I TTAA 4 cut(s) 39, 104, 294, 408
TscAI CASTG 1 cut(s) 346
TseFI GTSAC 2 cut(s) 44, 127
Tsp45I GTSAC 2 cut(s) 44, 127
TspDTI ATGAA 2 cut(s) 33, 219
TspGWI ACGGA 1 cut(s) 488
TspRI CASTG 1 cut(s) 346
VpaK11BI GGWCC 1 cut(s) 63
XapI RAATTY 2 cut(s) 236, 518
XcmI CCANNNNNNNNNTGG 1 cut(s) 322
ZrmI AGTACT 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.