Rh7DG103400

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000526
Physical Location & Seq
Forward (+)
8785190 .. 8791938
6749 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG103400.1

Sequence Viewer

Length: 5103 bp
ATGGCGACGACGATGACGCCAAGGGAACACATAGAAGATATACGGAAGACCAAGTTCTCCATCGGAGAAAAGCTCAACCCTTTAACGGAGGATCTTCACCAGGCCGTCAAGAACCTCTCCGGCGAGCTTTACGCTAAAGACGTCCACTTTTTCATGGAACTCGTTCAGAATGCAGAAGACAATGAGTACTCGGAGGGGGTTGATCCATCCCTTGAGTTTGTGATTACATACCGCGATATAACGGCTACAGGGGCTCCAGCCACATTGCTGGTTTTCAATAATGAAAGGGGGTTTTCTCCCAAAAATATTGACTCCATTTGCAGTGTTGGACGGTCCACCAAGAAAGGCAACAGGAAGCGTGGTTATATTGGGGAGAAAGGAATTGGCTTCAAGAGTGTGTTTCTGATCACTGCTCAGCCTTACATATTTAGTAATGGTTATCAGATAAGGTTCAGCGAAGAACCTTGTTCACATTGCAATGTTGGCTACATCGTACCTGAATGGGTTGAGCAGAATCCCACTCTATCTGATATAGAAAAGATATATGGTTCTGCTACTGCCCTTCCTACCACAACACTGATCTTACCTCTGAAGCCCGACAAGGTTGAACCTGTGAAGAAACAACTCTCGATCATTCATCCAGAAGTTCTCTTGTTCCTATCAAAGATTAGGCGGCTCTCTGTTAGGGAAGACAAAGAGGACCCAAGGCAGAACACTGTGAGTGCAATTGAGATAGCACGTGAGACTGAACATGCGCAAAGGAAGAACATCGATGCTCAATCCTACACTCTGCATCTTTCAGCGGAAGAAAATGGTGGAAATGGAGAATGCAGCTACTACATGTGGAAACAAAAGTTTCCTGTCAGGCAGGAATTCAGAGTTGAGAGGAGAATGGAGGTGGATGAGTGGGTGATCTCTCTTGCATTTCCAATTGGAGAACGTCTTCAAAGAGCAAGGAACTCTTCGCCTGGTGTTTATGCATTTCTTCCCACAGAAATGGTCACCAACTTTCCCTTCATAATACAGGCGGATTTTCTTTTAGCATCTTCAAGAGAGACAATCCTACTTGACAACAAGTGGAATCAAGGGATTCTTGAGTGTGTGCCCACCGCATTTTCCCATGCTTTTAACTCTCTTGTGAAAACTGTGGAAGGTGCGCCAACATCAAGTTTGCCTCCTATGTTCAGATTCTTGCCTGTTCAGGTTTCTTCCTGTAAAGAATTAAATGCTGTAAGGGATTCGATAAAAGCAAAGATGGTTGAGGAAGACATTATACCAGTTGAGCCCGAAATGGAGCAAAAGTTCTTCCACAAGCCTCGTGAAGTGGGAAGATTAATGCCTGCCTTTTGGTCTATTTTGAGGAAGGCGAGGGTTCAAGGTACTGTAAGCTTGATTAAGCTCTCATCACATGGAAGATTTGTTCTGTCTTATTCCTTTGACAGCAAGGAATATGATCCTATACTAAGTTTCCTTGGAGTTGAACCAGTGAATGATGACTGGTATGTTAAATGCATACAGGGTACTTCTAATCTTGTTGGTGGAGTGTCAGAAGATCTGTACTTAGAGCTTCTACAGTTTATCGCTGAAAATTGGGGTTCTAAATTTGGTCACACCAACATGAAGAACATACCATTGATAAAATATGCAGACTCTGGAAATGAATCTTTGTGCAGCATAAGCCAAATCCAGAAAGGGGAATGTAGAGTTTGCTTATCGAGCAAATATTCTCGTGTTTCATGGCTGATTGATTGCAACCGGGAATTTAATTCTGTGACTCGTCTTCTCTTTATGCCAAAAGCCACTCAGGAAGCCATCTGGACATGCTCTGGGAAGGATACATTGAAGAAATGGCTAGCTGATCAAGCTAAGGTTGTAGATGTTGATGTCAATCAATATGCAGTTGATCTCCTTCAGAATTCTCTTAATGAGCGGAAGCTTGTTATTGCATATGTTCATTTCTTATATCACTCGGTTGATAATGATTTTATCTCAAGTCGGGAAGTTCACCGTTTATGTGGAATTATGCCACTAGTGGATGGCTATGGGGACATCATCAGGGGTAGAAAAGGGGTTATTGTTCCTGCCAATGGAAGCAAATGGGCGGGATTGACTGATTCTAATTTGTGGAGAAAAGAAGGCTATGTTGAATTAGGAGAGCATTATATGAATTCTGGCTATTTTGCTGGTAAGTATACACTGGAGAAGAAGCTTCTTGAGTTCCTTATAGAACATGCTGCTGCTTTGGATGTCCCTCATATTTCTGCTCCAAGTGATCGTATCTCGTCTGTATCTGCACCACTAACTAAGCAGAATGCCTTTTTGCTTTTGGATTGGATTCGTCAACTGAATTATAAAAGGGTTGTCATCCCTCAGAAGTTCTTGGCTTGCATAAAGGAAGGTAGTTGGCTGAAAGTTACTTTGAATGGCTCTCCTGGTTATAGGCCACCATCCCAGTCATTTCTGCTTAACTCATCATGGGGAAATACTTTGCAGAATGGATCCGTCTCTGTTGACATTCCATTGATTGATACGAGCTTTTATGGTGAGTGCATTAGTGGATACAAGGAGGAGCTAAAGACAATTGGGGTCATGTTTGAATATAGCGAAGCATGTGACTTCATTGGGAAGCATTTCATGTCTCTGGCAGCTTCATCCTCTTTAACTAGAGGAAATGTGCATTCCATTCTGCGTTTCATCAGGTTTTTAAGGGACAAACTTCTTCCTCCGGCCGATTTTATCTCTGCTATTAAGAAAGGGAACTGGCTGAAGACTGCCGTTGGTTACAGGTCTCCTGTAGGATCTGTTTTGTCAGATAAGGAGTGGAATGTTGCCTCCAAAATTAGCAACATTCCCTTCATTGATCAGGACTTTTATGGTGATGAAATCTGTAATTTCAGAACTGAACTTGAGTTGCTCGGTGTTGTAATCTCCTTCAACCACCAACTTATAATTGACAACCTGAAGTCACCATCTTCCATAACTTATTGGTCACCTCAGGTTGTCATCTTAATGCTCAACTGTATGGCTCTATCCAAGTCATCTGATAAACTTGTCAATGCATTGAAAGGAGCCAAGTGCCTGAAGACAAGTATTGGTTACAAGTGTCCAGGGGAATGTCTCTTGTTTCATCACGAATGGGGTTGCATTCTTCAGGTCGTCGATGGTCTTCCCTTGATTGATCATGAATTCTATGGGAGCAACATTTTCCTGTACATAAATGAACTGAAAAAAATTGGTGTGGTGGTTGATTTTGAAGGGGCTGCAAAAGTATTTGCTCGATATTTCAGGCACTATGCATCATCAGCTTCCATCACCAAGGAAAATGTTGCATCATTGCTTCTGTGTTACAGAGCGCTGAAGGGGACTCCATATAAATTTCCTACTGATCTTAGGAGCTGCATTGGTGAGGTCAAGTGGTTGCGTAGTCGTTTTGGTGATTTCAGATGTCCAAGGGAGTGCATTCTCTTTAGTACCGAGTGGGAATCTATATCCCCAATAACTATGCTTCCTTTCATTGATGATTCTGACAGTTACTATGGAAAGAGCATACATGAGTACAGAAAAGAGCTGAAGAGCTTGGGGGTTGTCGTCGAATACAAGGAAGGAGTTAAGTTTGTGGCTTCTGGTCTTTATCTCCCTAGGGATGTCAGCCGTATTTTTCCTGTAAATGCACTTGCGTTGCTGGAATACATACGAGTTTTCCAGGAGAAAGGTTACGCCTTTCTTGATAATTCTGATTTCATGAAGAAAGTTTCTCAACCATGGATAAAGACTCATGCTGGTTATAGGTCTCCCAAAGAGTCTTTGTTGTTTGATTCCAAGTTTGGCTTATATTTGAAGCGGACTGATGGGCCTTTCATTGATGAAGAATTCTATGGTACTAAGATTGCATCTTACAAAAAAGAGCTGTCTGCATTGGGGGTTATTGTTGAATTTGAGAAAGGATGTCCCCTGATTGCCAGCCACCTTGATGTTCATTATGAGATTGAAGCATTTGTTCGGCTGTATAATTACTTGAGTGCATTCAAGTGGGAACCAGACACTGACGCTGCTCGAAGGATCTGGATTCCAAAAGGAGAGTGGGTTCGTCCTGATGATTGTGTTATATATGACAAGGATGGGCTCTTTGGTTTACAATTGACTGTTTTGGAGAAACATTATGAACAGAAACTTCTATTTTTCTTCTCCAGTGCGTTTAGAGTTAAATCTCATCCTTCAGTTGATGATTACTTGAAGGTTTGGAAGGTTTGGGAAAGTTCTGGAAGTGCATTGTCGCATGCTGATTGTTGCAAGTTTTGGAGTTATATCTCGAAGCACTGGAATTCAAAGACAGAAAAGACTGTTGCTGATGCCTTGGTGAAAGTACCTGTCAGTTCTGGATCTGAAGGAATCTTGCTGTGCAACAAGTCTGATGTTTTCATTGCTGATGACCTTCAACTGAAGTATCTATTTGAACAGTCTTCTCCTGTGTCTATTTTTGTTTGGTACCCTCAGCCAAGTTTGCCTTCCATTCCAAGATCCAAGTTGCTTGACATTTATCGAAGAATTGGTGTTCGCACTATATCTGAATCTGTACAGAAGGAAGAGGCGTCACTTGCAGATGATGTTGAAGTAGTCTCTTTGCCGAGGGAAAAGCTGATTAAGAAAGCGCTGTTCAGGCTGATTCTTGGTTTTCTTGCTGCTCCTCCCATGGAAATGGAAGCTGAGAAGAGGCTTGAAGTTGTTCAATTCCTTGTGAATGTTACTGTGGTGGTTACAGCTGAACCAATCTCTGTAAGCTATAATCTACCTTTATCTTCTGGGAAAGTTTTGAATGTGAGAGGAAGCCGAAAAATACGGTTTGATAGAGAAAATTCAAAGATCTTCACTCAGAAAATGGACAAGTCTGGAGGGCAGAAGAGTATCATTGAGTTTGCTACTTTTTTCTCCGAAGCGATATCTGAGAGTGTGTTATGGGAGAGCACTGATCATATAGATTCACTCTCTGAGCTGATCAAGATGGCTTATTTGCTAGACTTCAATGAGGAAGCAGTTGATTTCTTGATGAAGTCCAAGAATTTGCAAACTTTTATGGAGGATGAGGAGTTTCTCAAGACTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1700

Amino Acids

193.09

Weight (kDa)

6.67

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 30 - 146 3.8e-11 Sacsin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28020 AT3G48770 AT3G48770
fragaria_vesca FvH4_5g09020 FvH4_5g09020
malus_domestica MD06G1092400.v1.1 MD06G1134600.v1.1 MD06G1134700.v1.1 MD06G1134800.v1.1 MD06G1135200.v1.1 MD06G1135400.v1.1 MD06G1135500.v1.1 MD08G1137600.v1.1 MD15G1395100.v1.1
prunus_persica Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145400_v2.0.a1 Prupe.5G145600_v2.0.a1 Prupe.5G145800_v2.0.a1 Prupe.5G145900_v2.0.a1
pyrus_communis pycom06g12550 pycom06g12570 pycom06g12580 pycom06g12590 pycom06g12610 pycom06g12630 pycom06g12640 pycom06g12650 pycom08g06280
rosa_chinensis RchiOBHm_Chr6g0249321 RchiOBHm_Chr6g0275111 RchiOBHm_Chr7g0189191 RchiOBHm_Chr7g0189261
rosa_laevigata RLG00000004622 RLG00000004623 RLG00000008237 RLG00000008238 RLG00000023303 RLG00000035187
rosa_multiflora Rmu_co8294311.1_g000001 Rmu_co8389833.1_g000001 Rmu_sc0001044.1_g000004 Rmu_sc0001329.1_g000011 Rmu_sc0006012.1_g000010 Rmu_sc0007286.1_g000032 Rmu_sc0009360.1_g000016 Rmu_sc0011817.1_g000001 Rmu_sc0015050.1_g000001
rosa_roxburghii Rroxscaffold_1G00064000 Rroxscaffold_3G00265460 Rroxscaffold_3G00265580 Rroxscaffold_3G00265640 Rroxscaffold_3G00265690 Rroxscaffold_3G00265700 Rroxscaffold_7G00193620 Rroxscaffold_7G00204550 Rroxscaffold_7G00217350
rosa_rugosa Rorug01G0465500 Rorug06G0088500 Rorug06G0496700 Rorug06G0496700
rosa_samantha Rh1AG064800 Rh2BG015100 Rh2BG493500 Rh2DG017300 Rh2DG502500 Rh4BG208500 Rh5BG528500 Rh5CG552900 Rh6CG207800 Rh6DG197900 Rh6DG198000 Rh7AG101300 Rh7AG101400 Rh7AG101600 Rh7BG104100 Rh7BG104200 Rh7CG105600 Rh7CG105700 Rh7DG103200 Rh7DG103400
rosa_wichuraiana Rw1G007300 Rw5G034670 Rw7G008750 Rw7G008780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2352, 2948
AatII GACGTC 1 cut(s) 144
Acc16I TGCGCA 1 cut(s) 756
Acc65I GGTACC 1 cut(s) 4485
AccB1I GGYRCC 1 cut(s) 4485
AccBSI CCGCTC 1 cut(s) 1930
AccI GTMKAC 1 cut(s) 2192
AccII CGCG 1 cut(s) 234
AciI CCGC 8 cut(s) 232, 673, 803, 1028, 1110, 1930, 2102, 3841
AclWI GGATC 9 cut(s) 99, 197, 1448, 2493, 2506, 2806, 4067, 4387, 4512
AcoI YGGCCR 1 cut(s) 2727
AcvI CACGTG 1 cut(s) 740
AcyI GRCGYC 3 cut(s) 17, 141, 4589
AfeI AGCGCT 2 cut(s) 3354, 4650
AfiI CCNNNNNNNGG 3 cut(s) 85, 1693, 2087
AflIII ACRYGT 1 cut(s) 840
AhlI ACTAGT 1 cut(s) 2029
AjnI CCWGG 5 cut(s) 99, 967, 2431, 3106, 3702
AjuI GAANNNNNNNTTGG 4 cut(s) 998, 1030, 4489, 4521
AloI GAACNNNNNNTCC 6 cut(s) 1286, 1318, 1404, 1436, 4068, 4100
Alw21I GWGCWC 1 cut(s) 4964
Alw26I GTCTC 8 cut(s) 737, 1049, 2509, 2643, 2793, 3122, 3795, 4621
AlwI GGATC 9 cut(s) 99, 197, 1448, 2493, 2506, 2806, 4067, 4387, 4512
AlwNI CAGNNNCTG 2 cut(s) 1652, 4043
Aor51HI AGCGCT 2 cut(s) 3354, 4650
AoxI GGCC 4 cut(s) 102, 2441, 2727, 3851
ApeKI GCWGC 9 cut(s) 831, 1671, 2234, 2237, 2645, 3260, 3396, 4049, 4679
AseI ATTAAT 1 cut(s) 1334
Asp700I GAANNNNTTC 4 cut(s) 162, 942, 2630, 4722
Asp718I GGTACC 1 cut(s) 4485
AspA2I CCTAGG 1 cut(s) 3638
AspLEI GCGC 4 cut(s) 757, 1159, 3355, 4651
AspS9I GGNCC 3 cut(s) 333, 700, 3851
AsuC2I CCSGG 1 cut(s) 1757
AsuNHI GCTAGC 1 cut(s) 1852
AvaII GGWCC 2 cut(s) 333, 700
AvrII CCTAGG 1 cut(s) 3638
AxyI CCTNAGG 1 cut(s) 2994
BaeGI GKGCMC 1 cut(s) 1107
BaeI ACNNNNGTAYC 2 cut(s) 4427, 4460
BamHI GGATCC 1 cut(s) 2498
BanI GGYRCC 1 cut(s) 4485
BanII GRGCYC 3 cut(s) 256, 1287, 4125
BarI GAAGNNNNNNTAC 2 cut(s) 1257, 1289
BauI CACGAG 2 cut(s) 1317, 1728
BbrPI CACGTG 1 cut(s) 740
Bbv12I GWGCWC 1 cut(s) 4964
BbvCI CCTCAGC 1 cut(s) 4491
BbvI GCAGC 9 cut(s) 843, 1683, 2221, 2224, 2657, 3247, 3383, 4036, 4666
BceAI ACGGC 4 cut(s) 89, 258, 2759, 3636
BciT130I CCWGG 5 cut(s) 101, 969, 2433, 3108, 3704
BciVI GTATCC 2 cut(s) 1828, 2552
BclI TGATCA 6 cut(s) 405, 1858, 2860, 3178, 4966, 4992
BcnI CCSGG 1 cut(s) 1757
BcoDI GTCTC 8 cut(s) 737, 1049, 2509, 2643, 2793, 3122, 3795, 4621
BcuI ACTAGT 1 cut(s) 2029
BfaI CTAG 6 cut(s) 1853, 2030, 2664, 3639, 5012, 5101
BfmI CTRYAG 3 cut(s) 246, 1571, 2793
BfoI RGCGCY 2 cut(s) 3356, 4652
BfuI GTATCC 2 cut(s) 1828, 2552
BglII AGATCT 2 cut(s) 1552, 4860
BlnI CCTAGG 1 cut(s) 3638
BlpI GCTNAGC 1 cut(s) 414
BmcAI AGTACT 1 cut(s) 188
Bme1390I CCNGG 6 cut(s) 101, 969, 1757, 2433, 3108, 3704
Bme18I GGWCC 2 cut(s) 333, 700
BmgT120I GGNCC 3 cut(s) 333, 700, 3851
BmiI GGNNCC 6 cut(s) 255, 702, 2500, 3070, 4035, 4487
BmrFI CCNGG 6 cut(s) 101, 969, 1757, 2433, 3108, 3704
BmrI ACTGGG 1 cut(s) 2446
BmsI GCATC 7 cut(s) 763, 802, 1052, 3305, 3338, 3899, 4339
BmtI GCTAGC 1 cut(s) 1856
BmuI ACTGGG 1 cut(s) 2446
BplI GAGNNNNNCTC 2 cut(s) 57, 89
BpmI CTGGAG 4 cut(s) 240, 2219, 4171, 4908
Bpu10I CCTNAGC 2 cut(s) 1866, 4491
Bpu1102I GCTNAGC 1 cut(s) 414
BpuEI CTTGAG 7 cut(s) 233, 1115, 1975, 2234, 2927, 4036, 5075
BpuMI CCSGG 1 cut(s) 1757
Bsa29I ATCGAT 1 cut(s) 771
BsaAI YACGTR 1 cut(s) 740
BsaBI GATNNNNATC 1 cut(s) 1887
BsaHI GRCGYC 3 cut(s) 17, 141, 4589
BsaI GGTCTC 2 cut(s) 2793, 3795
BsaXI ACNNNNNCTCC 6 cut(s) 238, 268, 1286, 1316, 4068, 4098
Bsc4I CCNNNNNNNGG 3 cut(s) 85, 1693, 2087
Bse1I ACTGG 8 cut(s) 1277, 1484, 1502, 2202, 2452, 2765, 4188, 4322
Bse21I CCTNAGG 1 cut(s) 2994
Bse3DI GCAATG 5 cut(s) 263, 472, 484, 3332, 4419
Bse8I GATNNNNATC 1 cut(s) 1887
BseBI CCWGG 5 cut(s) 101, 969, 2433, 3108, 3704
BseCI ATCGAT 1 cut(s) 771
BseJI GATNNNNATC 1 cut(s) 1887
BseLI CCNNNNNNNGG 3 cut(s) 85, 1693, 2087
BseMI GCAATG 5 cut(s) 263, 472, 484, 3332, 4419
BseMII CTCAG 9 cut(s) 428, 1817, 2384, 3008, 4505, 4695, 4883, 4932, 4977
BseNI ACTGG 8 cut(s) 1277, 1484, 1502, 2202, 2452, 2765, 4188, 4322
BseRI GAGGAG 4 cut(s) 901, 2582, 4674, 5096
BseSI GKGCMC 1 cut(s) 1107
BseX3I CGGCCG 1 cut(s) 2727
BseXI GCAGC 9 cut(s) 843, 1683, 2221, 2224, 2657, 3247, 3383, 4036, 4666
BsgI GTGCAG 2 cut(s) 1690, 2277
Bsh1236I CGCG 1 cut(s) 234
Bsh1285I CGRYCG 1 cut(s) 2730
BshFI GGCC 4 cut(s) 104, 2443, 2729, 3853
BshNI GGYRCC 1 cut(s) 4485
BshVI ATCGAT 1 cut(s) 771
BsiEI CGRYCG 1 cut(s) 2730
BsiHKAI GWGCWC 1 cut(s) 4964
BsiSI CCGG 3 cut(s) 120, 1756, 2726
BslFI GGGAC 5 cut(s) 2060, 2234, 2723, 3376, 3933
BslI CCNNNNNNNGG 3 cut(s) 85, 1693, 2087
BsmAI GTCTC 8 cut(s) 737, 1049, 2509, 2643, 2793, 3122, 3795, 4621
BsmBI CGTCTC 1 cut(s) 2509
BsmFI GGGAC 5 cut(s) 2060, 2234, 2723, 3376, 3933
BsmI GAATGC 7 cut(s) 175, 833, 2317, 2677, 3144, 3459, 4022
BsnI GGCC 4 cut(s) 104, 2443, 2729, 3853
Bso31I GGTCTC 2 cut(s) 2793, 3795
Bsp1286I GDGCHC 5 cut(s) 256, 1107, 1287, 4125, 4964
Bsp1407I TGTACA 2 cut(s) 3210, 4573
Bsp1720I GCTNAGC 1 cut(s) 414
Bsp19I CCATGG 2 cut(s) 3761, 4689
BspACI CCGC 8 cut(s) 232, 673, 803, 1028, 1110, 1930, 2102, 3841
BspANI GGCC 4 cut(s) 104, 2443, 2729, 3853
BspCNI CTCAG 9 cut(s) 427, 1816, 2383, 3007, 4504, 4696, 4882, 4933, 4978
BspDI ATCGAT 1 cut(s) 771
BspFNI CGCG 1 cut(s) 234
BspHI TCATGA 2 cut(s) 3181, 3741
BspLI GGNNCC 6 cut(s) 255, 702, 2500, 3070, 4035, 4487
BspOI GCTAGC 1 cut(s) 1856
BspPI GGATC 9 cut(s) 99, 197, 1448, 2493, 2506, 2806, 4067, 4387, 4512
BspQI GCTCTTC 1 cut(s) 3566
BspT107I GGYRCC 1 cut(s) 4485
BspTNI GGTCTC 2 cut(s) 2793, 3795
BsrBI CCGCTC 1 cut(s) 1930
BsrDI GCAATG 5 cut(s) 263, 472, 484, 3332, 4419
BsrGI TGTACA 2 cut(s) 3210, 4573
BsrI ACTGG 8 cut(s) 1277, 1484, 1502, 2202, 2452, 2765, 4188, 4322
BssNAI GTATAC 1 cut(s) 2193
BssNI GRCGYC 3 cut(s) 17, 141, 4589
BssSI CACGAG 2 cut(s) 1317, 1728
BssT1I CCWWGG 9 cut(s) 20, 704, 1471, 3315, 3449, 3638, 3761, 4353, 4689
Bst1107I GTATAC 1 cut(s) 2193
Bst2BI CACGAG 2 cut(s) 1317, 1728
Bst2UI CCWGG 5 cut(s) 101, 969, 2433, 3108, 3704
Bst6I CTCTTC 5 cut(s) 967, 3566, 4578, 4703, 4892
BstACI GRCGYC 3 cut(s) 17, 141, 4589
BstAUI TGTACA 2 cut(s) 3210, 4573
BstBAI YACGTR 1 cut(s) 740
BstC8I GCNNGC 6 cut(s) 125, 1341, 1854, 2386, 3961, 4278
BstDSI CCRYGG 2 cut(s) 3761, 4689
BstEII GGTNACC 2 cut(s) 1000, 2988
BstFNI CGCG 1 cut(s) 234
BstH2I RGCGCY 2 cut(s) 3356, 4652
BstHHI GCGC 4 cut(s) 757, 1159, 3355, 4651
BstMAI GTCTC 8 cut(s) 737, 1049, 2509, 2643, 2793, 3122, 3795, 4621
BstMCI CGRYCG 1 cut(s) 2730
BstMWI GCNNNNNNNGC 9 cut(s) 251, 483, 1677, 1716, 1862, 3302, 4501, 4595, 4657
BstNI CCWGG 5 cut(s) 101, 969, 2433, 3108, 3704
BstNSI RCATGY 6 cut(s) 755, 844, 1824, 2234, 2613, 4280
BstPI GGTNACC 2 cut(s) 1000, 2988
BstSCI CCNGG 6 cut(s) 99, 967, 1755, 2431, 3106, 3702
BstSFI CTRYAG 3 cut(s) 246, 1571, 2793
BstSLI GKGCMC 1 cut(s) 1107
BstUI CGCG 1 cut(s) 234
BstV1I GCAGC 9 cut(s) 843, 1683, 2221, 2224, 2657, 3247, 3383, 4036, 4666
BstX2I RGATCY 8 cut(s) 91, 1552, 2498, 2798, 4059, 4379, 4517, 4860
BstXI CCANNNNNNTGG 3 cut(s) 268, 997, 4696
BstYI RGATCY 8 cut(s) 91, 1552, 2498, 2798, 4059, 4379, 4517, 4860
BstZ17I GTATAC 1 cut(s) 2193
BstZI CGGCCG 1 cut(s) 2727
Bsu15I ATCGAT 1 cut(s) 771
Bsu36I CCTNAGG 1 cut(s) 2994
BsuI GTATCC 2 cut(s) 1828, 2552
BsuRI GGCC 4 cut(s) 104, 2443, 2729, 3853
BsuTUI ATCGAT 1 cut(s) 771
BtgI CCRYGG 2 cut(s) 3761, 4689
BtsI GCAGTG 2 cut(s) 328, 408
Cac8I GCNNGC 6 cut(s) 125, 1341, 1854, 2386, 3961, 4278
CaiI CAGNNNCTG 2 cut(s) 1652, 4043
CciI TCATGA 2 cut(s) 3181, 3741
CfoI GCGC 4 cut(s) 757, 1159, 3355, 4651
Cfr13I GGNCC 3 cut(s) 333, 700, 3851
ClaI ATCGAT 1 cut(s) 771
CseI GACGC 3 cut(s) 25, 4055, 4578
EaeI YGGCCR 1 cut(s) 2727
EagI CGGCCG 1 cut(s) 2727
Eam1104I CTCTTC 5 cut(s) 967, 3566, 4578, 4703, 4892
EarI CTCTTC 5 cut(s) 967, 3566, 4578, 4703, 4892
EciI GGCGGA 1 cut(s) 1043
EclXI CGGCCG 1 cut(s) 2727
Eco130I CCWWGG 9 cut(s) 20, 704, 1471, 3315, 3449, 3638, 3761, 4353, 4689
Eco24I GRGCYC 3 cut(s) 256, 1287, 4125
Eco31I GGTCTC 2 cut(s) 2793, 3795
Eco32I GATATC 1 cut(s) 4938
Eco47I GGWCC 2 cut(s) 333, 700
Eco47III AGCGCT 2 cut(s) 3354, 4650
Eco52I CGGCCG 1 cut(s) 2727
Eco72I CACGTG 1 cut(s) 740
Eco81I CCTNAGG 1 cut(s) 2994
Eco91I GGTNACC 2 cut(s) 1000, 2988
EcoO109I RGGNCCY 1 cut(s) 700
EcoO65I GGTNACC 2 cut(s) 1000, 2988
EcoRI GAATTC 6 cut(s) 872, 1915, 2167, 3185, 3869, 4321
EcoRII CCWGG 5 cut(s) 99, 967, 2431, 3106, 3702
EcoRV GATATC 1 cut(s) 4938
EcoT14I CCWWGG 9 cut(s) 20, 704, 1471, 3315, 3449, 3638, 3761, 4353, 4689
EcoT22I ATGCAT 4 cut(s) 982, 1514, 3061, 3298
EcoT38I GRGCYC 3 cut(s) 256, 1287, 4125
ErhI CCWWGG 9 cut(s) 20, 704, 1471, 3315, 3449, 3638, 3761, 4353, 4689
Esp3I CGTCTC 1 cut(s) 2509
FalI AAGNNNNNCTT 8 cut(s) 946, 978, 1077, 1109, 3812, 3844, 4489, 4521
FaqI GGGAC 5 cut(s) 2060, 2234, 2723, 3376, 3933
FauI CCCGC 1 cut(s) 2095
FauNDI CATATG 1 cut(s) 1948
FbaI TGATCA 6 cut(s) 405, 1858, 2860, 3178, 4966, 4992
FblI GTMKAC 1 cut(s) 2192
FriOI GRGCYC 3 cut(s) 256, 1287, 4125
FspBI CTAG 6 cut(s) 1853, 2030, 2664, 3639, 5012, 5101
FspI TGCGCA 1 cut(s) 756
GlaI GCGC 4 cut(s) 756, 1158, 3354, 4650
GsuI CTGGAG 4 cut(s) 240, 2219, 4171, 4908
HaeII RGCGCY 2 cut(s) 3356, 4652
HaeIII GGCC 4 cut(s) 104, 2443, 2729, 3853
HapII CCGG 3 cut(s) 120, 1756, 2726
HgaI GACGC 3 cut(s) 25, 4055, 4578
HhaI GCGC 4 cut(s) 757, 1159, 3355, 4651
Hin1I GRCGYC 3 cut(s) 17, 141, 4589
Hin6I GCGC 4 cut(s) 755, 1157, 3353, 4649
HinP1I GCGC 4 cut(s) 755, 1157, 3353, 4649
HincII GTYRAC 2 cut(s) 2342, 2512
HindII GTYRAC 2 cut(s) 2342, 2512
HindIII AAGCTT 3 cut(s) 1387, 1934, 2207
HpaII CCGG 3 cut(s) 120, 1756, 2726
Hpy166II GTNNAC 8 cut(s) 145, 336, 470, 2005, 2193, 2342, 2512, 4133
Hpy8I GTNNAC 8 cut(s) 145, 336, 470, 2005, 2193, 2342, 2512, 4133
Hpy99I CGWCG 4 cut(s) 10, 13, 3161, 3593
HpyCH4IV ACGT 3 cut(s) 141, 739, 940
HpyF10VI GCNNNNNNNGC 9 cut(s) 251, 483, 1677, 1716, 1862, 3302, 4501, 4595, 4657
HpySE526I ACGT 3 cut(s) 141, 739, 940
Hsp92I GRCGYC 3 cut(s) 17, 141, 4589
HspAI GCGC 4 cut(s) 755, 1157, 3353, 4649
KpnI GGTACC 1 cut(s) 4489
Ksp22I TGATCA 6 cut(s) 405, 1858, 2860, 3178, 4966, 4992
LguI GCTCTTC 1 cut(s) 3566
LmnI GCTCC 8 cut(s) 259, 1294, 2269, 2569, 3068, 3195, 3393, 4687
Lsp1109I GCAGC 9 cut(s) 843, 1683, 2221, 2224, 2657, 3247, 3383, 4036, 4666
LweI GCATC 7 cut(s) 763, 802, 1052, 3305, 3338, 3899, 4339
MaeI CTAG 6 cut(s) 1853, 2030, 2664, 3639, 5012, 5101
MaeII ACGT 3 cut(s) 141, 739, 940
MbiI CCGCTC 1 cut(s) 1930
MfeI CAATTG 4 cut(s) 726, 930, 2580, 4136
MflI RGATCY 8 cut(s) 91, 1552, 2498, 2798, 4059, 4379, 4517, 4860
MhlI GDGCHC 5 cut(s) 256, 1107, 1287, 4125, 4964
MlyI GAGTC 6 cut(s) 305, 1643, 1768, 3358, 3766, 3809
MmeI TCCRAC 1 cut(s) 307
Mph1103I ATGCAT 4 cut(s) 982, 1514, 3061, 3298
MroXI GAANNNNTTC 4 cut(s) 162, 942, 2630, 4722
MslI CAYNNNNRTG 7 cut(s) 477, 995, 1616, 3008, 3969, 4027, 4694
MspA1I CMGCKG 2 cut(s) 803, 4760
MspI CCGG 3 cut(s) 120, 1756, 2726
MspR9I CCNGG 6 cut(s) 101, 969, 1757, 2433, 3108, 3704
MunI CAATTG 4 cut(s) 726, 930, 2580, 4136
Mva1269I GAATGC 7 cut(s) 175, 833, 2317, 2677, 3144, 3459, 4022
MvaI CCWGG 5 cut(s) 101, 969, 2433, 3108, 3704
MvnI CGCG 1 cut(s) 234
MwoI GCNNNNNNNGC 9 cut(s) 251, 483, 1677, 1716, 1862, 3302, 4501, 4595, 4657
NciI CCSGG 1 cut(s) 1757
NcoI CCATGG 2 cut(s) 3761, 4689
NdeI CATATG 1 cut(s) 1948
NheI GCTAGC 1 cut(s) 1852
NlaIV GGNNCC 6 cut(s) 255, 702, 2500, 3070, 4035, 4487
NmeAIII GCCGAG 1 cut(s) 4650
NmuCI GTSAC 7 cut(s) 1000, 1607, 1771, 2612, 2964, 2988, 4590
NsbI TGCGCA 1 cut(s) 756
NsiI ATGCAT 4 cut(s) 982, 1514, 3061, 3298
NspI RCATGY 6 cut(s) 755, 844, 1824, 2234, 2613, 4280
PaeI GCATGC 1 cut(s) 4280
PagI TCATGA 2 cut(s) 3181, 3741
PciI ACATGT 1 cut(s) 840
PciSI GCTCTTC 1 cut(s) 3566
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PctI GAATGC 7 cut(s) 175, 833, 2317, 2677, 3144, 3459, 4022
PdmI GAANNNNTTC 4 cut(s) 162, 942, 2630, 4722
PfoI TCCNGGA 1 cut(s) 3702
PleI GAGTC 6 cut(s) 305, 1643, 1768, 3358, 3766, 3808
PmaCI CACGTG 1 cut(s) 740
PmlI CACGTG 1 cut(s) 740
PpsI GAGTC 6 cut(s) 305, 1643, 1768, 3358, 3766, 3808
Ppu21I YACGTR 1 cut(s) 740
PpuMI RGGWCCY 1 cut(s) 700
PscI ACATGT 1 cut(s) 840
PshBI ATTAAT 1 cut(s) 1334
PsiI TTATAA 2 cut(s) 2352, 2948
Psp5II RGGWCCY 1 cut(s) 700
Psp6I CCWGG 5 cut(s) 99, 967, 2431, 3106, 3702
PspCI CACGTG 1 cut(s) 740
PspEI GGTNACC 2 cut(s) 1000, 2988
PspGI CCWGG 5 cut(s) 99, 967, 2431, 3106, 3702
PspN4I GGNNCC 6 cut(s) 255, 702, 2500, 3070, 4035, 4487
PspPI GGNCC 3 cut(s) 333, 700, 3851
PspPPI RGGWCCY 1 cut(s) 700
PstNI CAGNNNCTG 2 cut(s) 1652, 4043
PsuI RGATCY 8 cut(s) 91, 1552, 2498, 2798, 4059, 4379, 4517, 4860
PvuII CAGCTG 1 cut(s) 4760
RseI CAYNNNNRTG 7 cut(s) 477, 995, 1616, 3008, 3969, 4027, 4694
SapI GCTCTTC 1 cut(s) 3566
Sau96I GGNCC 3 cut(s) 333, 700, 3851
ScaI AGTACT 1 cut(s) 188
SchI GAGTC 6 cut(s) 305, 1643, 1768, 3358, 3766, 3809
ScrFI CCNGG 6 cut(s) 101, 969, 1757, 2433, 3108, 3704
SduI GDGCHC 5 cut(s) 256, 1107, 1287, 4125, 4964
SfaNI GCATC 7 cut(s) 763, 802, 1052, 3305, 3338, 3899, 4339
SfcI CTRYAG 3 cut(s) 246, 1571, 2793
SinI GGWCC 2 cut(s) 333, 700
SmiMI CAYNNNNRTG 7 cut(s) 477, 995, 1616, 3008, 3969, 4027, 4694
SmlI CTYRAG 7 cut(s) 212, 1094, 1990, 2213, 2906, 4015, 5090
SmoI CTYRAG 7 cut(s) 212, 1094, 1990, 2213, 2906, 4015, 5090
SpeI ACTAGT 1 cut(s) 2029
SphI GCATGC 1 cut(s) 4280
SsiI CCGC 8 cut(s) 232, 673, 803, 1028, 1110, 1930, 2102, 3841
SspI AATATT 2 cut(s) 307, 1724
SspMI CTAG 6 cut(s) 1853, 2030, 2664, 3639, 5012, 5101
StyD4I CCNGG 6 cut(s) 99, 967, 1755, 2431, 3106, 3702
StyI CCWWGG 9 cut(s) 20, 704, 1471, 3315, 3449, 3638, 3761, 4353, 4689
TaiI ACGT 3 cut(s) 144, 742, 943
TatI WGTACW 5 cut(s) 186, 1557, 3210, 3555, 4573
TauI GCSGC 1 cut(s) 676
TseFI GTSAC 7 cut(s) 1000, 1607, 1771, 2612, 2964, 2988, 4590
TseI GCWGC 9 cut(s) 831, 1671, 2234, 2237, 2645, 3260, 3396, 4049, 4679
Tsp45I GTSAC 7 cut(s) 1000, 1607, 1771, 2612, 2964, 2988, 4590
TspGWI ACGGA 3 cut(s) 58, 101, 2491
VpaK11BI GGWCC 2 cut(s) 333, 700
VspI ATTAAT 1 cut(s) 1334
XceI RCATGY 6 cut(s) 755, 844, 1824, 2234, 2613, 4280
XcmI CCANNNNNNNNNTGG 1 cut(s) 4077
XmaJI CCTAGG 1 cut(s) 3638
XmiI GTMKAC 1 cut(s) 2192
XmnI GAANNNNTTC 4 cut(s) 162, 942, 2630, 4722
XspI CTAG 6 cut(s) 1853, 2030, 2664, 3639, 5012, 5101
ZraI GACGTC 1 cut(s) 142
ZrmI AGTACT 1 cut(s) 188
Zsp2I ATGCAT 4 cut(s) 982, 1514, 3061, 3298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.