Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0000473

Physical Location & Seq

Genomic Coordinates Forward (+)

25969035 .. 25972575

Gene Structure 3541 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G65010.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1737 bp

ATGTGTGGGATTCTCGCTGTTCTTGGTTGCATCGACAACTCTCAAGCTAAACGTTCTCGTATCATCGAACTCTCTCGCAGATTGAGGCACAGAGGTCCTGATTGGAGTGGACTCCATTGTTATGAAGATTGTTATCTTGCCCATGAGCGTTTAGCCATCATTGACCCTACTTCAGGAGACCAACCTCTCTATAACGAAGACAAGACCGTCGCTGTCACTGTAAATGGAGAGATATACAACCACAAGATTTTGCGTGAAAAGCTTAAGTCTCATCAGTTCCGTACTGGTAGTGACTGTGAAGTGATTGCACATCTTTACGAAGAACATGGAGAGGAATTTATCGACATGTTGGATGGAATGTTCGCGTTTGTCCTTCTTGATACTCGCGACAAAAGTTTTATTGCTGCAAGGGACGCTATTGGTATCACTCCACTTTACATTGGATGGGGTCTTGATGGTTCTGTCTGGTTTGCTTCGGAGATGAAAGCGCTTAGTGATGATTGTGAACAGTTTATGTCTTTTCCTCCTGGCCACATCTACTCAAGTAAACAAGGAGGGCTTAGGAGGTGGTACAATCCTCCGTGGTACAATGAGCAGGTTCCTTCAACCCCATATGATCCTTTAGTTCTGCGCAATGCTTTCGAGAAGGCTGTAATAAAGAGACTTATGACTGATGTGCCTTTTGGAGTTCTCCTATCTGGAGGATTGGACTCGTCTCTCGTTGCTGCAGTAGCATTACGCCATTTGGAAAAATCAGAAGCTGCTCGTCAATGGGGTTCACAATTGCACACGTTTTGCATCGGTTTGCAGGGATCGCCAGATCTTAAAGCTGGCAGAGAAGTTGCTGACTATCTTGGAACACGCCACCACGAGTTTCAGTTTACAGTTCAGGACGGGATAGACGCGATAGAGGAAGTCATTTACCATATTGAGACTTATGACGTTACTACAATAAGAGCTAGCACCCCAATGTTTCTTATGTCCAGAAAAATTAAATCTTTAGGTGTAAAGATGGTTCTTTCTGGGGAAGGTTCTGATGAAATACTGGGGGGATACTTGTACTTCCATAAGGCTCCCAACAAGAAAGAATTTCATGAAGAAACATGCCGAAAGATCAAAGCTCTCCACCAATTTGATTGTTTGAGAGCTAACAAATCAACTTCTGCGTGGGGTGTCGAAGCTCGTGTGCCTTTCCTAGATAAAGAATTTTTAAATGTTGCAATGAGCATCGATCCAGAGTGGAAGTTGATCAAGCCTGATCTCGGAAGGATCGAGAAGTGGGTGCTACGCAATGCCTTTGATGATGAAGAACGACCTTATCTACCAAAGCACATTCTATATAGACAGAAAGAACAGTTTAGTGATGGAGTTGGGTATAGCTGGATAGATGGTCTGAAAGATCATGCAAATAAACATGTCTCTGATACTATGCTGTCAAACGCAAGCTTTGTCTTCCCGGATAACACACCTCTGACAAAAGAAGCGTACTACTACAGAACCATCTTCGAGAAGTTCTTCCCGAAGAGTGCTGCTAGAGCGACTGTACCAGGAGGTCCAAGTATAGCTTGCAGTACCGCGAAAGCTGTAGAATGGGATGCAACTTGGTCAAAGAATCTTGATCCGTCAGGCCGTGCGGCTCTTGGAGTTCATGTTGCAGCTTATGAAGAGGATAAAGCAGCTGCTGCTGCTAAGGCTGGATCGGATTTAGTAGATCCTCTTCCTAAGAATGGAACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004066 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006082 GO:0006520 GO:0006528 GO:0006529 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009066 GO:0009067 GO:0009314 GO:0009416 GO:0009506 GO:0009628 GO:0009642 GO:0009646 GO:0009651 GO:0009987 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019752 GO:0030054 GO:0032787 GO:0034641 GO:0042538 GO:0042802 GO:0042803 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046983 GO:0050896 GO:0055044 GO:0071704 GO:0072330 GO:0097164 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00250 ko01100 ko01110 map00250 map01100 map01110

Pfam Domains

Protein Families

Asn_synthase GATase_7

Protein Analysis

578

Amino Acids

65.03

Weight (kDa)

6.01

Isoelectric Point (pI)

42.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_6	PF13522	33 - 160	7e-36	Glutamine amidotransferase domain
GATase_7	PF13537	48 - 165	5.9e-43	Glutamine amidotransferase domain
DUF3700	PF12481	112 - 194	5.9e-12	Aluminium induced protein
Asn_synthase	PF00733	210 - 361	7.4e-51	Asparagine synthase
Asn_synthase	PF00733	359 - 512	1.8e-33	Asparagine synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0000473)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10240 AT5G10240 AT5G65010 AT5G65010
fragaria_vesca	FvH4_1g20910 FvH4_1g20910 FvH4_1g29202 FvH4_3g23760 FvH4_3g42550
malus_domestica	MD01G1029700.v1.1 MD15G1324200.v1.1
prunus_persica	Prupe.6G186800_v2.0.a1 Prupe.6G186800_v2.0.a1 Prupe.6G186800_v2.0.a1
pyrus_communis	pycom01g05790
rosa_chinensis	RchiOBHm_Chr2g0113551 RchiOBHm_Chr2g0113591 RchiOBHm_Chr2g0113641 RchiOBHm_Chr2g0113651 RchiOBHm_Chr2g0113711 RchiOBHm_Chr2g0113721 RchiOBHm_Chr2g0113831 RchiOBHm_Chr2g0113841 RchiOBHm_Chr2g0113851 RchiOBHm_Chr2g0113911 RchiOBHm_Chr2g0113941 RchiOBHm_Chr2g0113991 RchiOBHm_Chr2g0114001 RchiOBHm_Chr2g0114041 RchiOBHm_Chr2g0114051 RchiOBHm_Chr2g0114061 RchiOBHm_Chr2g0114071 RchiOBHm_Chr2g0114081 RchiOBHm_Chr2g0114101 RchiOBHm_Chr2g0114131 RchiOBHm_Chr2g0114141 RchiOBHm_Chr2g0114261 RchiOBHm_Chr2g0114271 RchiOBHm_Chr2g0114291 RchiOBHm_Chr2g0124041
rosa_laevigata	RLG00000018073 RLG00000018076 RLG00000018078 RLG00000018084 RLG00000018086 RLG00000018088 RLG00000018094 RLG00000018095
rosa_multiflora	Rmu_sc0000881.1_g000007 Rmu_sc0000929.1_g000008 Rmu_sc0000929.1_g000017 Rmu_sc0000929.1_g000029 Rmu_sc0000929.1_g000039 Rmu_sc0001699.1_g000005 Rmu_sc0004283.1_g000004 Rmu_sc0007010.1_g000001 Rmu_sc0007415.1_g000028 Rmu_sc0007415.1_g000045 Rmu_sc0009639.1_g000009 Rmu_sc0010543.1_g000008 Rmu_sc0011941.1_g000022 Rmu_sc0018769.1_g000001
rosa_roxburghii	Rroxscaffold_2G00129150 Rroxscaffold_2G00130050 Rroxscaffold_2G00130070 Rroxscaffold_2G00130090
rosa_rugosa	Rorug02G0188100 Rorug02G0188100 Rorug02G0189700 Rorug02G0190000 Rorug02G0190000 Rorug03G0301100
rosa_samantha	Rh2AG242700 Rh2AG243100 Rh2AG245300 Rh2AG246800 Rh2BG256600 Rh2DG253800 Rh6CG172600
rosa_wichuraiana	Rw0G014630 Rw2G018450 Rw2G018500 Rw2G018980 Rw2G019030 Rw2G019050 Rw2G019100 Rw2G019130 Rw2G019190 Rw2G019420 Rw5G028430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	206
Acc16I	TGCGCA	1 cut(s)	632
Acc36I	ACCTGC	1 cut(s)	586
AccII	CGCG	4 cut(s)	365, 387, 905, 1577
AciI	CCGC	2 cut(s)	1575, 1634
AclI	AACGTT	1 cut(s)	52
AclWI	GGATC	7 cut(s)	611, 820, 1226, 1277, 1613, 1705, 1706
AcoI	YGGCCR	1 cut(s)	529
AcsI	RAATTY	3 cut(s)	335, 1088, 1205
AcuI	CTGAAG	1 cut(s)	156
AfaI	GTAC	7 cut(s)	283, 572, 587, 1061, 1487, 1545, 1573
AfeI	AGCGCT	1 cut(s)	489
AfiI	CCNNNNNNNGG	3 cut(s)	173, 1262, 1727
AflII	CTTAAG	1 cut(s)	263
AflIII	ACRYGT	3 cut(s)	345, 789, 1414
AgsI	TTSAA	1 cut(s)	606
AjnI	CCWGG	2 cut(s)	526, 1546
AloI	GAACNNNNNNTCC	2 cut(s)	344, 376
Alw26I	GTCTC	6 cut(s)	171, 273, 655, 720, 926, 1423
AlwI	GGATC	7 cut(s)	611, 820, 1226, 1277, 1613, 1705, 1706
AlwNI	CAGNNNCTG	2 cut(s)	761, 1682
Aor51HI	AGCGCT	1 cut(s)	489
AoxI	GGCC	2 cut(s)	529, 1627
ApeKI	GCWGC	9 cut(s)	404, 725, 761, 1529, 1655, 1676, 1679, 1682, 1685
ApoI	RAATTY	3 cut(s)	335, 1088, 1205
Asp700I	GAANNNNTTC	1 cut(s)	1514
AspLEI	GCGC	2 cut(s)	490, 633
AspS9I	GGNCC	2 cut(s)	95, 1553
AsuC2I	CCSGG	1 cut(s)	1457
AsuNHI	GCTAGC	1 cut(s)	959
AvaII	GGWCC	2 cut(s)	95, 1553
BalI	TGGCCA	1 cut(s)	531
BarI	GAAGNNNNNNTAC	2 cut(s)	1701, 1733
BauI	CACGAG	2 cut(s)	869, 1182
BbsI	GAAGAC	2 cut(s)	204, 1444
BbvI	GCAGC	9 cut(s)	391, 712, 748, 1516, 1666, 1667, 1669, 1672, 1688
BccI	CCATC	8 cut(s)	164, 347, 438, 449, 1006, 1358, 1382, 1508
BceAI	ACGGC	1 cut(s)	1614
BcgI	CGANNNNNNTGC	2 cut(s)	622, 656
BciT130I	CCWGG	2 cut(s)	528, 1548
BciVI	GTATCC	1 cut(s)	1046
BclI	TGATCA	1 cut(s)	1248
BcnI	CCSGG	1 cut(s)	1457
BcoDI	GTCTC	6 cut(s)	171, 273, 655, 720, 926, 1423
BfaI	CTAG	3 cut(s)	960, 1196, 1533
BfmI	CTRYAG	3 cut(s)	726, 1492, 1584
BfoI	RGCGCY	1 cut(s)	491
BfrI	CTTAAG	1 cut(s)	263
BfuAI	ACCTGC	1 cut(s)	586
BfuI	GTATCC	1 cut(s)	1046
BglII	AGATCT	1 cut(s)	820
Bme1390I	CCNGG	3 cut(s)	528, 1457, 1548
Bme18I	GGWCC	2 cut(s)	95, 1553
BmgT120I	GGNCC	2 cut(s)	95, 1553
BmiI	GGNNCC	2 cut(s)	600, 1074
BmrFI	CCNGG	3 cut(s)	528, 1457, 1548
BmrI	ACTGGG	1 cut(s)	1055
BmsI	GCATC	4 cut(s)	39, 807, 1236, 1585
BmtI	GCTAGC	1 cut(s)	963
BmuI	ACTGGG	1 cut(s)	1055
BpiI	GAAGAC	2 cut(s)	204, 1444
BpmI	CTGGAG	1 cut(s)	720
Bpu10I	CCTNAGC	2 cut(s)	560, 1689
BpuEI	CTTGAG	2 cut(s)	27, 526
BpuMI	CCSGG	1 cut(s)	1457
Bsa29I	ATCGAT	1 cut(s)	1230
BsaBI	GATNNNNATC	1 cut(s)	132
BsaI	GGTCTC	1 cut(s)	171
BsaJI	CCNNGG	1 cut(s)	581
BsaXI	ACNNNNNCTCC	2 cut(s)	1635, 1665
Bsc4I	CCNNNNNNNGG	3 cut(s)	173, 1262, 1727
Bse1I	ACTGG	2 cut(s)	289, 1050
Bse3DI	GCAATG	3 cut(s)	640, 1227, 1297
Bse8I	GATNNNNATC	1 cut(s)	132
BseBI	CCWGG	2 cut(s)	528, 1548
BseCI	ATCGAT	1 cut(s)	1230
BseDI	CCNNGG	1 cut(s)	581
BseGI	GGATG	3 cut(s)	358, 449, 1600
BseJI	GATNNNNATC	1 cut(s)	132
BseLI	CCNNNNNNNGG	3 cut(s)	173, 1262, 1727
BseMI	GCAATG	3 cut(s)	640, 1227, 1297
BseNI	ACTGG	2 cut(s)	289, 1050
BseXI	GCAGC	9 cut(s)	391, 712, 748, 1516, 1666, 1667, 1669, 1672, 1688
Bsh1236I	CGCG	4 cut(s)	365, 387, 905, 1577
BshFI	GGCC	2 cut(s)	531, 1629
BshVI	ATCGAT	1 cut(s)	1230
BsiSI	CCGG	1 cut(s)	1457
BslFI	GGGAC	1 cut(s)	425
BslI	CCNNNNNNNGG	3 cut(s)	173, 1262, 1727
BsmAI	GTCTC	6 cut(s)	171, 273, 655, 720, 926, 1423
BsmBI	CGTCTC	1 cut(s)	720
BsmFI	GGGAC	1 cut(s)	425
BsnI	GGCC	2 cut(s)	531, 1629
Bso31I	GGTCTC	1 cut(s)	171
Bsp68I	TCGCGA	1 cut(s)	387
BspACI	CCGC	2 cut(s)	1575, 1634
BspANI	GGCC	2 cut(s)	531, 1629
BspDI	ATCGAT	1 cut(s)	1230
BspFNI	CGCG	4 cut(s)	365, 387, 905, 1577
BspHI	TCATGA	1 cut(s)	1093
BspLI	GGNNCC	2 cut(s)	600, 1074
BspMAI	CTGCAG	1 cut(s)	730
BspMI	ACCTGC	1 cut(s)	586
BspOI	GCTAGC	1 cut(s)	963
BspPI	GGATC	7 cut(s)	611, 820, 1226, 1277, 1613, 1705, 1706
BspTI	CTTAAG	1 cut(s)	263
BspTNI	GGTCTC	1 cut(s)	171
BsrDI	GCAATG	3 cut(s)	640, 1227, 1297
BsrI	ACTGG	2 cut(s)	289, 1050
BssECI	CCNNGG	1 cut(s)	581
BssSI	CACGAG	2 cut(s)	869, 1182
Bst2BI	CACGAG	2 cut(s)	869, 1182
Bst2UI	CCWGG	2 cut(s)	528, 1548
Bst4CI	ACNGT	7 cut(s)	208, 220, 296, 510, 886, 1356, 1543
Bst6I	CTCTTC	3 cut(s)	1517, 1659, 1722
BstAFI	CTTAAG	1 cut(s)	263
BstAPI	GCANNNNNTGC	1 cut(s)	1682
BstC8I	GCNNGC	4 cut(s)	832, 961, 1444, 1567
BstDEI	CTNAG	4 cut(s)	491, 560, 1689, 1722
BstDSI	CCRYGG	1 cut(s)	581
BstENI	CCTNNNNNAGG	1 cut(s)	171
BstF5I	GGATG	3 cut(s)	358, 449, 1600
BstFNI	CGCG	4 cut(s)	365, 387, 905, 1577
BstH2I	RGCGCY	1 cut(s)	491
BstHHI	GCGC	2 cut(s)	490, 633
BstMAI	GTCTC	6 cut(s)	171, 273, 655, 720, 926, 1423
BstMWI	GCNNNNNNNGC	8 cut(s)	259, 413, 731, 814, 1535, 1682, 1685, 1691
BstNI	CCWGG	2 cut(s)	528, 1548
BstNSI	RCATGY	3 cut(s)	349, 1107, 1418
BstSCI	CCNGG	3 cut(s)	526, 1455, 1546
BstSFI	CTRYAG	3 cut(s)	726, 1492, 1584
BstUI	CGCG	4 cut(s)	365, 387, 905, 1577
BstV1I	GCAGC	9 cut(s)	391, 712, 748, 1516, 1666, 1667, 1669, 1672, 1688
BstV2I	GAAGAC	2 cut(s)	204, 1444
BstX2I	RGATCY	2 cut(s)	820, 1711
BstYI	RGATCY	2 cut(s)	820, 1711
Bsu15I	ATCGAT	1 cut(s)	1230
BsuI	GTATCC	1 cut(s)	1046
BsuRI	GGCC	2 cut(s)	531, 1629
BsuTUI	ATCGAT	1 cut(s)	1230
BtgI	CCRYGG	1 cut(s)	581
BtsCI	GGATG	3 cut(s)	358, 449, 1600
BtsIMutI	CAGTG	1 cut(s)	216
BtuMI	TCGCGA	1 cut(s)	387
BveI	ACCTGC	1 cut(s)	586
Cac8I	GCNNGC	4 cut(s)	832, 961, 1444, 1567
CaiI	CAGNNNCTG	2 cut(s)	761, 1682
CciI	TCATGA	1 cut(s)	1093
CfoI	GCGC	2 cut(s)	490, 633
Cfr13I	GGNCC	2 cut(s)	95, 1553
ClaI	ATCGAT	1 cut(s)	1230
CseI	GACGC	2 cut(s)	422, 911
Csp6I	GTAC	7 cut(s)	282, 571, 586, 1060, 1486, 1544, 1572
CviAII	CATG	8 cut(s)	143, 326, 346, 1094, 1104, 1403, 1415, 1649
CviQI	GTAC	7 cut(s)	282, 571, 586, 1060, 1486, 1544, 1572
DdeI	CTNAG	4 cut(s)	491, 560, 1689, 1722
DraI	TTTAAA	1 cut(s)	1212
DrdI	GACNNNNNNGTC	1 cut(s)	206
DseDI	GACNNNNNNGTC	1 cut(s)	206
EaeI	YGGCCR	1 cut(s)	529
Eam1104I	CTCTTC	3 cut(s)	1517, 1659, 1722
EarI	CTCTTC	3 cut(s)	1517, 1659, 1722
Eco31I	GGTCTC	1 cut(s)	171
Eco47I	GGWCC	2 cut(s)	95, 1553
Eco47III	AGCGCT	1 cut(s)	489
Eco57I	CTGAAG	1 cut(s)	156
EcoNI	CCTNNNNNAGG	1 cut(s)	171
EcoO109I	RGGNCCY	1 cut(s)	95
EcoRII	CCWGG	2 cut(s)	526, 1546
Esp3I	CGTCTC	1 cut(s)	720
FaeI	CATG	8 cut(s)	146, 329, 349, 1097, 1107, 1406, 1418, 1652
FalI	AAGNNNNNCTT	4 cut(s)	543, 575, 1549, 1581
FaqI	GGGAC	1 cut(s)	425
FatI	CATG	8 cut(s)	142, 325, 345, 1093, 1103, 1402, 1414, 1648
FauNDI	CATATG	1 cut(s)	613
FbaI	TGATCA	1 cut(s)	1248
FokI	GGATG	3 cut(s)	365, 456, 1607
FspBI	CTAG	3 cut(s)	960, 1196, 1533
FspI	TGCGCA	1 cut(s)	632
GlaI	GCGC	2 cut(s)	489, 632
GsuI	CTGGAG	1 cut(s)	720
HaeII	RGCGCY	1 cut(s)	491
HaeIII	GGCC	2 cut(s)	531, 1629
HapII	CCGG	1 cut(s)	1457
HgaI	GACGC	2 cut(s)	422, 911
HhaI	GCGC	2 cut(s)	490, 633
Hin1II	CATG	8 cut(s)	146, 329, 349, 1097, 1107, 1406, 1418, 1652
Hin6I	GCGC	2 cut(s)	488, 631
HinP1I	GCGC	2 cut(s)	488, 631
HindIII	AAGCTT	2 cut(s)	260, 1444
HinfI	GANTC	4 cut(s)	10, 111, 710, 1612
HpaII	CCGG	1 cut(s)	1457
Hpy166II	GTNNAC	5 cut(s)	110, 506, 548, 779, 882
Hpy188I	TCNGA	8 cut(s)	478, 757, 1036, 1265, 1395, 1423, 1473, 1702
Hpy8I	GTNNAC	5 cut(s)	110, 506, 548, 779, 882
Hpy99I	CGWCG	1 cut(s)	212
HpyAV	CCTTC	5 cut(s)	383, 612, 640, 1022, 1260
HpyCH4III	ACNGT	7 cut(s)	208, 220, 296, 510, 886, 1356, 1543
HpyCH4IV	ACGT	3 cut(s)	52, 791, 942
HpyF10VI	GCNNNNNNNGC	8 cut(s)	259, 413, 731, 814, 1535, 1682, 1685, 1691
HpyF3I	CTNAG	4 cut(s)	491, 560, 1689, 1722
HpySE526I	ACGT	3 cut(s)	52, 791, 942
Hsp92II	CATG	8 cut(s)	146, 329, 349, 1097, 1107, 1406, 1418, 1652
HspAI	GCGC	2 cut(s)	488, 631
Ksp22I	TGATCA	1 cut(s)	1248
LmnI	GCTCC	1 cut(s)	1078
Lsp1109I	GCAGC	9 cut(s)	391, 712, 748, 1516, 1666, 1667, 1669, 1672, 1688
LweI	GCATC	4 cut(s)	39, 807, 1236, 1585
MaeI	CTAG	3 cut(s)	960, 1196, 1533
MaeII	ACGT	3 cut(s)	52, 791, 942
MaeIII	GTNAC	3 cut(s)	214, 290, 943
MfeI	CAATTG	1 cut(s)	782
MflI	RGATCY	2 cut(s)	820, 1711
MlsI	TGGCCA	1 cut(s)	531
MluCI	AATT	6 cut(s)	335, 782, 990, 1088, 1130, 1205
MluNI	TGGCCA	1 cut(s)	531
MlyI	GAGTC	2 cut(s)	105, 704
MmeI	TCCRAC	1 cut(s)	330
Mox20I	TGGCCA	1 cut(s)	531
MroXI	GAANNNNTTC	1 cut(s)	1514
MscI	TGGCCA	1 cut(s)	531
MseI	TTAA	4 cut(s)	264, 825, 993, 1211
MslI	CAYNNNNRTG	2 cut(s)	120, 968
Msp20I	TGGCCA	1 cut(s)	531
MspA1I	CMGCKG	1 cut(s)	1679
MspCI	CTTAAG	1 cut(s)	263
MspI	CCGG	1 cut(s)	1457
MspR9I	CCNGG	3 cut(s)	528, 1457, 1548
MunI	CAATTG	1 cut(s)	782
MvaI	CCWGG	2 cut(s)	528, 1548
MvnI	CGCG	4 cut(s)	365, 387, 905, 1577
MwoI	GCNNNNNNNGC	8 cut(s)	259, 413, 731, 814, 1535, 1682, 1685, 1691
NciI	CCSGG	1 cut(s)	1457
NdeI	CATATG	1 cut(s)	613
NheI	GCTAGC	1 cut(s)	959
NlaIII	CATG	8 cut(s)	146, 329, 349, 1097, 1107, 1406, 1418, 1652
NlaIV	GGNNCC	2 cut(s)	600, 1074
NmuCI	GTSAC	2 cut(s)	214, 290
NruI	TCGCGA	1 cut(s)	387
NsbI	TGCGCA	1 cut(s)	632
NspI	RCATGY	3 cut(s)	349, 1107, 1418
PagI	TCATGA	1 cut(s)	1093
PciI	ACATGT	2 cut(s)	345, 1414
PcsI	WCGNNNNNNNCGW	1 cut(s)	1269
PdmI	GAANNNNTTC	1 cut(s)	1514
PfeI	GAWTC	2 cut(s)	10, 1612
PfoI	TCCNGGA	1 cut(s)	1455
PleI	GAGTC	2 cut(s)	105, 704
PpsI	GAGTC	2 cut(s)	105, 704
PpuMI	RGGWCCY	1 cut(s)	95
PscI	ACATGT	2 cut(s)	345, 1414
Psp1406I	AACGTT	1 cut(s)	52
Psp5II	RGGWCCY	1 cut(s)	95
Psp6I	CCWGG	2 cut(s)	526, 1546
PspGI	CCWGG	2 cut(s)	526, 1546
PspN4I	GGNNCC	2 cut(s)	600, 1074
PspPI	GGNCC	2 cut(s)	95, 1553
PspPPI	RGGWCCY	1 cut(s)	95
PsrI	GAACNNNNNNTAC	2 cut(s)	999, 1031
PstI	CTGCAG	1 cut(s)	730
PstNI	CAGNNNCTG	2 cut(s)	761, 1682
PsuI	RGATCY	2 cut(s)	820, 1711
PvuII	CAGCTG	1 cut(s)	1679
RruI	TCGCGA	1 cut(s)	387
RsaI	GTAC	7 cut(s)	283, 572, 587, 1061, 1487, 1545, 1573
RsaNI	GTAC	7 cut(s)	282, 571, 586, 1060, 1486, 1544, 1572
RseI	CAYNNNNRTG	2 cut(s)	120, 968
SaqAI	TTAA	4 cut(s)	264, 825, 993, 1211
Sau96I	GGNCC	2 cut(s)	95, 1553
SchI	GAGTC	2 cut(s)	105, 704
ScrFI	CCNGG	3 cut(s)	528, 1457, 1548
SfaNI	GCATC	4 cut(s)	39, 807, 1236, 1585
SfcI	CTRYAG	3 cut(s)	726, 1492, 1584
SinI	GGWCC	2 cut(s)	95, 1553
SmiMI	CAYNNNNRTG	2 cut(s)	120, 968
SmlI	CTYRAG	3 cut(s)	42, 263, 541
SmoI	CTYRAG	3 cut(s)	42, 263, 541
Sse9I	AATT	6 cut(s)	335, 782, 990, 1088, 1130, 1205
SsiI	CCGC	2 cut(s)	1575, 1634
SspMI	CTAG	3 cut(s)	960, 1196, 1533
StyD4I	CCNGG	3 cut(s)	526, 1455, 1546
TaaI	ACNGT	7 cut(s)	208, 220, 296, 510, 886, 1356, 1543
TaiI	ACGT	3 cut(s)	55, 794, 945
TaqI	TCGA	8 cut(s)	33, 66, 342, 642, 1176, 1230, 1272, 1506
TasI	AATT	6 cut(s)	335, 782, 990, 1088, 1130, 1205
TatI	WGTACW	1 cut(s)	1059
TauI	GCSGC	1 cut(s)	1637
TfiI	GAWTC	2 cut(s)	10, 1612
Tru1I	TTAA	4 cut(s)	264, 825, 993, 1211
Tru9I	TTAA	4 cut(s)	264, 825, 993, 1211
TscAI	CASTG	1 cut(s)	223
TseFI	GTSAC	2 cut(s)	214, 290
TseI	GCWGC	9 cut(s)	404, 725, 761, 1529, 1655, 1676, 1679, 1682, 1685
Tsp45I	GTSAC	2 cut(s)	214, 290
TspDTI	ATGAA	8 cut(s)	138, 497, 1053, 1082, 1110, 1320, 1637, 1677
TspGWI	ACGGA	3 cut(s)	269, 570, 1611
TspRI	CASTG	1 cut(s)	223
Vha464I	CTTAAG	1 cut(s)	263
VpaK11BI	GGWCC	2 cut(s)	95, 1553
XagI	CCTNNNNNAGG	1 cut(s)	171
XapI	RAATTY	3 cut(s)	335, 1088, 1205
XceI	RCATGY	3 cut(s)	349, 1107, 1418
XmnI	GAANNNNTTC	1 cut(s)	1514
XspI	CTAG	3 cut(s)	960, 1196, 1533

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.