Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0000473

Physical Location & Seq

Genomic Coordinates Forward (+)

26081683 .. 26086573

Gene Structure 4891 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ48729

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1188 bp

ATGATCCCATTGTTTTACGCTAGGCTTTTGAGAAGTTGGGCAGTGTTAAAGAGACTTACGACAGATGTACCATTCGGTGTTCTTTTGTCCGGAGGATTGGACTCGTCACTCGTTGCTGCTGTGGCTTGTCGCTATTTGGCTAAATCAGAAGCTGCACGTCAGTGGGGGTCACAATTACATACCTTTTGCGTTGGCTTAGAGGGTTCTCCAGATTTAAAAGCAGCAAGAGAAGTTGCTGATTATCTTGGAACTCATCATCACAAGTTTCACTTTACTGTTCACGAAGGCATAGATGCACTTGAAGAAGTTATCTACCATATTGAGACATATGATGTGACCACTGTCAGAGCCAGCACTCCAATGTTTCTTATGTCTCGTAAGATAAAATCTCTGGGAGTAAAAATGGTCCTTTCTGGTGAAGGTTCAGATGAAATCTTTGGTGGCTACTTGTATTTCCACAAGGCACCAAACAAGGAGGAGTTTCACCAAGAAACATGCCAGAATATTAAAGCTCTTCACCTGTGCGACTGTCTGAGGGCCAACAAATCGACTTCAGCTTGGGGTGTTGAGGCTCGAGTACCGTTTCTTGACAAAGAATTCATCAAGACTGCTATGGACATTAAACTTCCCCCCCCCCCCCCCCCCCCCCTCCAATTTTATACACAGTTCAAGTATTGTAATAAGGCTGATCTTGGGAGAATTGAGAATTGGGTCTTGTGCAAAGCATTTGATGATGATCAGAAGCCATATTTACTAAAGCACAACTTGCACAGGCAGAAGGGGCGGTTTAGTGATGGTGTCGGTTACAGCTGGATTGATGGCTTGAAAGATCATGCCAACAAACAAGTAACAGATTCAATGTTGATGAACGCAAGCTTTGTCTACCCTGAAAACACTCCGACTACAAAAGAAGGATACTACTATAGGACTATCTTTGAGAAATATTTTCCAAAGAATGCTGCGAGATCGACAGTTCCCGGTGGTCCAAGTGTGGCATGCAGCACAGCAAAAGCTGTGGAGTGGGACGCGGAATGGTCCAAGAACCCTGATCCATCTGGTCGTGCTTCACTTGGTGTACATGCAGCTGCATATGAGGAATCCAAAAATGTGAAGACTGGAAGTCTTGTGAGTGACTCTCCACAGAAACTTGTAAAAGGGGTTGCAGAGAAAGTGGCTGCAGTAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004066 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006082 GO:0006520 GO:0006528 GO:0006529 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009066 GO:0009067 GO:0009314 GO:0009416 GO:0009506 GO:0009628 GO:0009642 GO:0009646 GO:0009651 GO:0009987 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019752 GO:0030054 GO:0032787 GO:0034641 GO:0042538 GO:0042802 GO:0042803 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046983 GO:0050896 GO:0055044 GO:0071704 GO:0072330 GO:0097164 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00250 ko01100 ko01110 map00250 map01100 map01110

Pfam Domains

Protein Families

Asn_synthase GATase_7

Protein Analysis

395

Amino Acids

44.08

Weight (kDa)

8.45

Isoelectric Point (pI)

41.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asn_synthase	PF00733	13 - 158	3.3e-49	Asparagine synthase
Asn_synthase	PF00733	156 - 321	2e-19	Asparagine synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000473)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10240 AT5G10240 AT5G65010 AT5G65010
fragaria_vesca	FvH4_1g20910 FvH4_1g20910 FvH4_1g29202 FvH4_3g23760 FvH4_3g42550
malus_domestica	MD01G1029700.v1.1 MD15G1324200.v1.1
prunus_persica	Prupe.6G186800_v2.0.a1 Prupe.6G186800_v2.0.a1 Prupe.6G186800_v2.0.a1
pyrus_communis	pycom01g05790
rosa_chinensis	RchiOBHm_Chr2g0113551 RchiOBHm_Chr2g0113591 RchiOBHm_Chr2g0113641 RchiOBHm_Chr2g0113651 RchiOBHm_Chr2g0113711 RchiOBHm_Chr2g0113721 RchiOBHm_Chr2g0113831 RchiOBHm_Chr2g0113841 RchiOBHm_Chr2g0113851 RchiOBHm_Chr2g0113911 RchiOBHm_Chr2g0113941 RchiOBHm_Chr2g0113991 RchiOBHm_Chr2g0114001 RchiOBHm_Chr2g0114041 RchiOBHm_Chr2g0114051 RchiOBHm_Chr2g0114061 RchiOBHm_Chr2g0114071 RchiOBHm_Chr2g0114081 RchiOBHm_Chr2g0114101 RchiOBHm_Chr2g0114131 RchiOBHm_Chr2g0114141 RchiOBHm_Chr2g0114261 RchiOBHm_Chr2g0114271 RchiOBHm_Chr2g0114291 RchiOBHm_Chr2g0124041
rosa_laevigata	RLG00000018073 RLG00000018076 RLG00000018078 RLG00000018084 RLG00000018086 RLG00000018088 RLG00000018094 RLG00000018095
rosa_multiflora	Rmu_sc0000881.1_g000007 Rmu_sc0000929.1_g000008 Rmu_sc0000929.1_g000017 Rmu_sc0000929.1_g000029 Rmu_sc0000929.1_g000039 Rmu_sc0001699.1_g000005 Rmu_sc0004283.1_g000004 Rmu_sc0007010.1_g000001 Rmu_sc0007415.1_g000028 Rmu_sc0007415.1_g000045 Rmu_sc0009639.1_g000009 Rmu_sc0010543.1_g000008 Rmu_sc0011941.1_g000022 Rmu_sc0018769.1_g000001
rosa_roxburghii	Rroxscaffold_2G00129150 Rroxscaffold_2G00130050 Rroxscaffold_2G00130070 Rroxscaffold_2G00130090
rosa_rugosa	Rorug02G0188100 Rorug02G0188100 Rorug02G0189700 Rorug02G0190000 Rorug02G0190000 Rorug03G0301100
rosa_samantha	Rh2AG242700 Rh2AG243100 Rh2AG245300 Rh2AG246800 Rh2BG256600 Rh2DG253800 Rh6CG172600
rosa_wichuraiana	Rw0G014630 Rw2G018450 Rw2G018500 Rw2G018980 Rw2G019030 Rw2G019050 Rw2G019100 Rw2G019130 Rw2G019190 Rw2G019420 Rw5G028430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	463
AccI	GTMKAC	1 cut(s)	882
AccII	CGCG	1 cut(s)	1028
AccIII	TCCGGA	1 cut(s)	89
AciI	CCGC	2 cut(s)	784, 1028
AclWI	GGATC	1 cut(s)	1043
AcsI	RAATTY	1 cut(s)	596
AcuI	CTGAAG	1 cut(s)	537
AdeI	CACNNNGTG	1 cut(s)	1073
AfaI	GTAC	3 cut(s)	69, 579, 1077
AgsI	TTSAA	4 cut(s)	302, 670, 826, 858
AhdI	GACNNNNNGTC	1 cut(s)	1119
AjiI	CACGTC	1 cut(s)	158
AjuI	GAANNNNNNNTTGG	2 cut(s)	691, 723
AleI	CACNNNNGTG	1 cut(s)	160
AluBI	AGCT	7 cut(s)	152, 512, 557, 810, 876, 1013, 1085
AluI	AGCT	7 cut(s)	152, 512, 557, 810, 876, 1013, 1085
Alw26I	GTCTC	3 cut(s)	46, 317, 378
AlwI	GGATC	1 cut(s)	1043
AlwNI	CAGNNNCTG	1 cut(s)	152
Ama87I	CYCGRG	1 cut(s)	573
Aor13HI	TCCGGA	1 cut(s)	89
AoxI	GGCC	1 cut(s)	537
ApeKI	GCWGC	8 cut(s)	116, 152, 221, 959, 999, 1082, 1085, 1175
ApoI	RAATTY	1 cut(s)	596
AspS9I	GGNCC	4 cut(s)	406, 537, 983, 1035
AsuC2I	CCSGG	1 cut(s)	978
AsuHPI	GGTGA	3 cut(s)	428, 476, 509
AvaI	CYCGRG	1 cut(s)	573
AvaII	GGWCC	3 cut(s)	406, 983, 1035
BaeI	ACNNNNGTAYC	2 cut(s)	51, 84
BanI	GGYRCC	1 cut(s)	463
BbsI	GAAGAC	1 cut(s)	1118
BbvI	GCAGC	8 cut(s)	103, 139, 233, 946, 1011, 1072, 1094, 1162
BccI	CCATC	3 cut(s)	788, 812, 1060
BciVI	GTATCC	1 cut(s)	908
BclI	TGATCA	1 cut(s)	736
BcnI	CCSGG	1 cut(s)	978
BcoDI	GTCTC	3 cut(s)	46, 317, 378
BfaI	CTAG	1 cut(s)	21
BfmI	CTRYAG	2 cut(s)	922, 1176
BfuI	GTATCC	1 cut(s)	908
BisI	GCNGC	8 cut(s)	117, 153, 222, 960, 1000, 1083, 1086, 1176
BlsI	GCNGC	8 cut(s)	118, 154, 223, 961, 1001, 1084, 1087, 1177
Bme1390I	CCNGG	1 cut(s)	978
Bme18I	GGWCC	3 cut(s)	406, 983, 1035
BmeRI	GACNNNNNGTC	1 cut(s)	1119
BmeT110I	CYCGRG	1 cut(s)	573
BmgBI	CACGTC	1 cut(s)	158
BmgT120I	GGNCC	4 cut(s)	406, 537, 983, 1035
BmiI	GGNNCC	1 cut(s)	465
BmrFI	CCNGG	1 cut(s)	978
BmsI	GCATC	1 cut(s)	283
BoxI	GACNNNNGTC	1 cut(s)	341
BpiI	GAAGAC	1 cut(s)	1118
BplI	GAGNNNNNCTC	2 cut(s)	1120, 1152
BpmI	CTGGAG	1 cut(s)	192
BpuMI	CCSGG	1 cut(s)	978
BsaBI	GATNNNNATC	1 cut(s)	735
BsaWI	WCCGGW	1 cut(s)	89
Bse1I	ACTGG	1 cut(s)	1120
Bse8I	GATNNNNATC	1 cut(s)	735
BseAI	TCCGGA	1 cut(s)	89
BseJI	GATNNNNATC	1 cut(s)	735
BseMII	CTCAG	1 cut(s)	524
BseNI	ACTGG	1 cut(s)	1120
BseRI	GAGGAG	1 cut(s)	491
BseXI	GCAGC	8 cut(s)	103, 139, 233, 946, 1011, 1072, 1094, 1162
BsgI	GTGCAG	1 cut(s)	138
Bsh1236I	CGCG	1 cut(s)	1028
BshFI	GGCC	1 cut(s)	539
BshNI	GGYRCC	1 cut(s)	463
BsiHKCI	CYCGRG	1 cut(s)	573
BsiSI	CCGG	2 cut(s)	90, 978
BslFI	GGGAC	1 cut(s)	1037
BsmAI	GTCTC	3 cut(s)	46, 317, 378
BsmFI	GGGAC	1 cut(s)	1037
BsmI	GAATGC	1 cut(s)	961
BsnI	GGCC	1 cut(s)	539
BsoBI	CYCGRG	1 cut(s)	573
Bsp13I	TCCGGA	1 cut(s)	89
Bsp1407I	TGTACA	1 cut(s)	1075
Bsp143I	GATC	6 cut(s)	3, 688, 736, 829, 965, 1048
BspACI	CCGC	2 cut(s)	784, 1028
BspANI	GGCC	1 cut(s)	539
BspCNI	CTCAG	1 cut(s)	525
BspEI	TCCGGA	1 cut(s)	89
BspFNI	CGCG	1 cut(s)	1028
BspLI	GGNNCC	1 cut(s)	465
BspMAI	CTGCAG	1 cut(s)	1180
BspPI	GGATC	1 cut(s)	1043
BspQI	GCTCTTC	1 cut(s)	519
BspT107I	GGYRCC	1 cut(s)	463
BsrGI	TGTACA	1 cut(s)	1075
BsrI	ACTGG	1 cut(s)	1120
BssMI	GATC	6 cut(s)	3, 688, 736, 829, 965, 1048
Bst4CI	ACNGT	6 cut(s)	277, 343, 530, 582, 666, 973
Bst6I	CTCTTC	1 cut(s)	519
BstAPI	GCANNNNNTGC	1 cut(s)	766
BstAUI	TGTACA	1 cut(s)	1075
BstC8I	GCNNGC	3 cut(s)	352, 874, 997
BstDEI	CTNAG	2 cut(s)	196, 533
BstFNI	CGCG	1 cut(s)	1028
BstKTI	GATC	6 cut(s)	6, 691, 739, 832, 968, 1051
BstMAI	GTCTC	3 cut(s)	46, 317, 378
BstMBI	GATC	6 cut(s)	3, 688, 736, 829, 965, 1048
BstMWI	GCNNNNNNNGC	3 cut(s)	122, 766, 781
BstNSI	RCATGY	3 cut(s)	498, 999, 1082
BstPAI	GACNNNNGTC	1 cut(s)	341
BstSCI	CCNGG	1 cut(s)	976
BstSFI	CTRYAG	2 cut(s)	922, 1176
BstUI	CGCG	1 cut(s)	1028
BstV1I	GCAGC	8 cut(s)	103, 139, 233, 946, 1011, 1072, 1094, 1162
BstV2I	GAAGAC	1 cut(s)	1118
BsuI	GTATCC	1 cut(s)	908
BsuRI	GGCC	1 cut(s)	539
BtrI	CACGTC	1 cut(s)	158
BtsI	GCAGTG	1 cut(s)	48
BtsIMutI	CAGTG	3 cut(s)	48, 167, 339
Cac8I	GCNNGC	3 cut(s)	352, 874, 997
CaiI	CAGNNNCTG	1 cut(s)	152
Cfr13I	GGNCC	4 cut(s)	406, 537, 983, 1035
CseI	GACGC	1 cut(s)	1034
Csp6I	GTAC	3 cut(s)	68, 578, 1076
CspCI	CAANNNNNGTGG	2 cut(s)	996, 1031
CviAII	CATG	4 cut(s)	495, 833, 996, 1079
CviQI	GTAC	3 cut(s)	68, 578, 1076
DdeI	CTNAG	2 cut(s)	196, 533
DpnI	GATC	6 cut(s)	5, 690, 738, 831, 967, 1050
DpnII	GATC	6 cut(s)	3, 688, 736, 829, 965, 1048
DraI	TTTAAA	1 cut(s)	216
DraIII	CACNNNGTG	1 cut(s)	1073
DriI	GACNNNNNGTC	1 cut(s)	1119
Eam1104I	CTCTTC	1 cut(s)	519
Eam1105I	GACNNNNNGTC	1 cut(s)	1119
EarI	CTCTTC	1 cut(s)	519
Eco47I	GGWCC	3 cut(s)	406, 983, 1035
Eco57I	CTGAAG	1 cut(s)	537
Eco88I	CYCGRG	1 cut(s)	573
EcoRI	GAATTC	1 cut(s)	596
FaeI	CATG	4 cut(s)	498, 836, 999, 1082
FalI	AAGNNNNNCTT	4 cut(s)	254, 286, 749, 781
FaqI	GGGAC	1 cut(s)	1037
FatI	CATG	4 cut(s)	494, 832, 995, 1078
FauNDI	CATATG	2 cut(s)	328, 1090
FbaI	TGATCA	1 cut(s)	736
FblI	GTMKAC	1 cut(s)	882
Fnu4HI	GCNGC	8 cut(s)	117, 153, 222, 960, 1000, 1083, 1086, 1176
Fsp4HI	GCNGC	8 cut(s)	117, 153, 222, 960, 1000, 1083, 1086, 1176
FspBI	CTAG	1 cut(s)	21
GluI	GCNGC	8 cut(s)	117, 153, 222, 960, 1000, 1083, 1086, 1176
GsuI	CTGGAG	1 cut(s)	192
HaeIII	GGCC	1 cut(s)	539
HapII	CCGG	2 cut(s)	90, 978
HgaI	GACGC	1 cut(s)	1034
Hin1II	CATG	4 cut(s)	498, 836, 999, 1082
HindIII	AAGCTT	1 cut(s)	874
HinfI	GANTC	4 cut(s)	101, 854, 1097, 1133
HpaII	CCGG	2 cut(s)	90, 978
HphI	GGTGA	3 cut(s)	428, 476, 509
Hpy166II	GTNNAC	3 cut(s)	280, 883, 1076
Hpy188I	TCNGA	6 cut(s)	148, 347, 427, 534, 741, 900
Hpy188III	TCNNGA	5 cut(s)	90, 209, 281, 587, 604
Hpy8I	GTNNAC	3 cut(s)	280, 883, 1076
HpyAV	CCTTC	4 cut(s)	278, 413, 772, 905
HpyCH4III	ACNGT	6 cut(s)	277, 343, 530, 582, 666, 973
HpyCH4IV	ACGT	1 cut(s)	157
HpyCH4V	TGCA	9 cut(s)	155, 296, 720, 769, 999, 1082, 1088, 1163, 1178
HpyF10VI	GCNNNNNNNGC	3 cut(s)	122, 766, 781
HpyF3I	CTNAG	2 cut(s)	196, 533
HpySE526I	ACGT	1 cut(s)	157
Hsp92II	CATG	4 cut(s)	498, 836, 999, 1082
Kpn2I	TCCGGA	1 cut(s)	89
Ksp22I	TGATCA	1 cut(s)	736
Kzo9I	GATC	6 cut(s)	3, 688, 736, 829, 965, 1048
LguI	GCTCTTC	1 cut(s)	519
Lsp1109I	GCAGC	8 cut(s)	103, 139, 233, 946, 1011, 1072, 1094, 1162
LweI	GCATC	1 cut(s)	283
MaeI	CTAG	1 cut(s)	21
MaeII	ACGT	1 cut(s)	157
MaeIII	GTNAC	6 cut(s)	105, 168, 334, 803, 847, 1130
MalI	GATC	6 cut(s)	5, 690, 738, 831, 967, 1050
MboI	GATC	6 cut(s)	3, 688, 736, 829, 965, 1048
MboII	GAAGA	3 cut(s)	314, 506, 1123
MluCI	AATT	5 cut(s)	173, 596, 653, 699, 706
MlyI	GAGTC	2 cut(s)	95, 1127
MmeI	TCCRAC	1 cut(s)	923
MnlI	CCTC	7 cut(s)	86, 193, 469, 528, 562, 659, 1087
MroI	TCCGGA	1 cut(s)	89
MseI	TTAA	4 cut(s)	47, 215, 507, 621
MslI	CAYNNNNRTG	2 cut(s)	160, 359
MspA1I	CMGCKG	2 cut(s)	810, 1085
MspI	CCGG	2 cut(s)	90, 978
MspR9I	CCNGG	1 cut(s)	978
Mva1269I	GAATGC	1 cut(s)	961
MvnI	CGCG	1 cut(s)	1028
MwoI	GCNNNNNNNGC	3 cut(s)	122, 766, 781
NciI	CCSGG	1 cut(s)	978
NdeI	CATATG	2 cut(s)	328, 1090
NdeII	GATC	6 cut(s)	3, 688, 736, 829, 965, 1048
NlaIII	CATG	4 cut(s)	498, 836, 999, 1082
NlaIV	GGNNCC	1 cut(s)	465
NmuCI	GTSAC	4 cut(s)	105, 168, 334, 1130
NspI	RCATGY	3 cut(s)	498, 999, 1082
OliI	CACNNNNGTG	1 cut(s)	160
PaeI	GCATGC	1 cut(s)	999
PaeR7I	CTCGAG	1 cut(s)	573
PciSI	GCTCTTC	1 cut(s)	519
PctI	GAATGC	1 cut(s)	961
PfeI	GAWTC	2 cut(s)	854, 1097
PkrI	GCNGC	8 cut(s)	118, 154, 223, 961, 1001, 1084, 1087, 1177
PleI	GAGTC	2 cut(s)	95, 1127
PpsI	GAGTC	2 cut(s)	95, 1127
PshAI	GACNNNNGTC	1 cut(s)	341
PspN4I	GGNNCC	1 cut(s)	465
PspPI	GGNCC	4 cut(s)	406, 537, 983, 1035
PspXI	VCTCGAGB	1 cut(s)	573
PstI	CTGCAG	1 cut(s)	1180
PstNI	CAGNNNCTG	1 cut(s)	152
PvuII	CAGCTG	2 cut(s)	810, 1085
RsaI	GTAC	3 cut(s)	69, 579, 1077
RsaNI	GTAC	3 cut(s)	68, 578, 1076
RseI	CAYNNNNRTG	2 cut(s)	160, 359
SapI	GCTCTTC	1 cut(s)	519
SaqAI	TTAA	4 cut(s)	47, 215, 507, 621
SatI	GCNGC	8 cut(s)	117, 153, 222, 960, 1000, 1083, 1086, 1176
Sau3AI	GATC	6 cut(s)	3, 688, 736, 829, 965, 1048
Sau96I	GGNCC	4 cut(s)	406, 537, 983, 1035
SchI	GAGTC	2 cut(s)	95, 1127
ScrFI	CCNGG	1 cut(s)	978
SfaNI	GCATC	1 cut(s)	283
SfcI	CTRYAG	2 cut(s)	922, 1176
Sfr274I	CTCGAG	1 cut(s)	573
SinI	GGWCC	3 cut(s)	406, 983, 1035
SlaI	CTCGAG	1 cut(s)	573
SmiMI	CAYNNNNRTG	2 cut(s)	160, 359
SmlI	CTYRAG	1 cut(s)	573
SmoI	CTYRAG	1 cut(s)	573
SphI	GCATGC	1 cut(s)	999
Sse9I	AATT	5 cut(s)	173, 596, 653, 699, 706
SsiI	CCGC	2 cut(s)	784, 1028
SspI	AATATT	2 cut(s)	505, 944
SspMI	CTAG	1 cut(s)	21
StyD4I	CCNGG	1 cut(s)	976
TaaI	ACNGT	6 cut(s)	277, 343, 530, 582, 666, 973
TaiI	ACGT	1 cut(s)	160
TaqI	TCGA	3 cut(s)	548, 574, 968
TasI	AATT	5 cut(s)	173, 596, 653, 699, 706
TatI	WGTACW	1 cut(s)	1075
TfiI	GAWTC	2 cut(s)	854, 1097
Tru1I	TTAA	4 cut(s)	47, 215, 507, 621
Tru9I	TTAA	4 cut(s)	47, 215, 507, 621
TscAI	CASTG	3 cut(s)	48, 167, 346
TseFI	GTSAC	4 cut(s)	105, 168, 334, 1130
TseI	GCWGC	8 cut(s)	116, 152, 221, 959, 999, 1082, 1085, 1175
Tsp45I	GTSAC	4 cut(s)	105, 168, 334, 1130
TspDTI	ATGAA	3 cut(s)	444, 589, 881
TspRI	CASTG	3 cut(s)	48, 167, 346
VpaK11BI	GGWCC	3 cut(s)	406, 983, 1035
XapI	RAATTY	1 cut(s)	596
XceI	RCATGY	3 cut(s)	498, 999, 1082
XhoI	CTCGAG	1 cut(s)	573
XmiI	GTMKAC	1 cut(s)	882
XspI	CTAG	1 cut(s)	21

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.