Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0000473

Physical Location & Seq

Genomic Coordinates Forward (+)

66161375 .. 66167553

Gene Structure 6179 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00130050.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 426 bp

ATGTTTCTTACGTCTCGTAAAATTAAATCTCTGGGTGTAAAAATGGTCCTTTCTGGTGAAGGTTCGGATGAAATCTTTGGTGGCTACTTGTATTTCCACAAGGCACCAAACAAGGAGGAGTTTCACCAAGAAACATGCCAGAAGATTAAAGGTCTTCATCTGTATGACTGTCTGAGGGCCAACAAATCGACATCAGCTTGGGGTGTTGAGGCTCGTGTACCGTTTCTTGACAAAGAATTTATCAAGACTGCTATGGATATTGATCCAGAATGGAAAATGATCAGGCCTGATCTTCGAAGAACTGAGAAGTGGGTCTTACGCAAAGCATTTGATGATGATCAGAAGCCATATTACCAAAGCACATCTTATACAGGCAGAAGGAGCAGTTTAGTGATGGTGTCGGTTACAGCTGGATTGACGGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004066 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006082 GO:0006520 GO:0006528 GO:0006529 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009066 GO:0009067 GO:0009314 GO:0009416 GO:0009506 GO:0009628 GO:0009642 GO:0009646 GO:0009651 GO:0009987 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019752 GO:0030054 GO:0032787 GO:0034641 GO:0042538 GO:0042802 GO:0042803 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046983 GO:0050896 GO:0055044 GO:0071704 GO:0072330 GO:0097164 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00250 ko01100 ko01110 map00250 map01100 map01110

Pfam Domains

Protein Families

Asn_synthase GATase_7

Protein Analysis

141

Amino Acids

16.25

Weight (kDa)

9.2

Isoelectric Point (pI)

47.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asn_synthase	PF00733	1 - 38	9.8e-11	Asparagine synthase
Asn_synthase	PF00733	36 - 112	9.5e-20	Asparagine synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0000473)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10240 AT5G10240 AT5G65010 AT5G65010
fragaria_vesca	FvH4_1g20910 FvH4_1g20910 FvH4_1g29202 FvH4_3g23760 FvH4_3g42550
malus_domestica	MD01G1029700.v1.1 MD15G1324200.v1.1
prunus_persica	Prupe.6G186800_v2.0.a1 Prupe.6G186800_v2.0.a1 Prupe.6G186800_v2.0.a1
pyrus_communis	pycom01g05790
rosa_chinensis	RchiOBHm_Chr2g0113551 RchiOBHm_Chr2g0113591 RchiOBHm_Chr2g0113641 RchiOBHm_Chr2g0113651 RchiOBHm_Chr2g0113711 RchiOBHm_Chr2g0113721 RchiOBHm_Chr2g0113831 RchiOBHm_Chr2g0113841 RchiOBHm_Chr2g0113851 RchiOBHm_Chr2g0113911 RchiOBHm_Chr2g0113941 RchiOBHm_Chr2g0113991 RchiOBHm_Chr2g0114001 RchiOBHm_Chr2g0114041 RchiOBHm_Chr2g0114051 RchiOBHm_Chr2g0114061 RchiOBHm_Chr2g0114071 RchiOBHm_Chr2g0114081 RchiOBHm_Chr2g0114101 RchiOBHm_Chr2g0114131 RchiOBHm_Chr2g0114141 RchiOBHm_Chr2g0114261 RchiOBHm_Chr2g0114271 RchiOBHm_Chr2g0114291 RchiOBHm_Chr2g0124041
rosa_laevigata	RLG00000018073 RLG00000018076 RLG00000018078 RLG00000018084 RLG00000018086 RLG00000018088 RLG00000018094 RLG00000018095
rosa_multiflora	Rmu_sc0000881.1_g000007 Rmu_sc0000929.1_g000008 Rmu_sc0000929.1_g000017 Rmu_sc0000929.1_g000029 Rmu_sc0000929.1_g000039 Rmu_sc0001699.1_g000005 Rmu_sc0004283.1_g000004 Rmu_sc0007010.1_g000001 Rmu_sc0007415.1_g000028 Rmu_sc0007415.1_g000045 Rmu_sc0009639.1_g000009 Rmu_sc0010543.1_g000008 Rmu_sc0011941.1_g000022 Rmu_sc0018769.1_g000001
rosa_roxburghii	Rroxscaffold_2G00129150 Rroxscaffold_2G00130050 Rroxscaffold_2G00130070 Rroxscaffold_2G00130090
rosa_rugosa	Rorug02G0188100 Rorug02G0188100 Rorug02G0189700 Rorug02G0190000 Rorug02G0190000 Rorug03G0301100
rosa_samantha	Rh2AG242700 Rh2AG243100 Rh2AG245300 Rh2AG246800 Rh2BG256600 Rh2DG253800 Rh6CG172600
rosa_wichuraiana	Rw0G014630 Rw2G018450 Rw2G018500 Rw2G018980 Rw2G019030 Rw2G019050 Rw2G019100 Rw2G019130 Rw2G019190 Rw2G019420 Rw5G028430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	103
AclWI	GGATC	1 cut(s)	257
AcsI	RAATTY	1 cut(s)	236
AfaI	GTAC	1 cut(s)	219
AluBI	AGCT	2 cut(s)	197, 410
AluI	AGCT	2 cut(s)	197, 410
Alw26I	GTCTC	1 cut(s)	18
AlwI	GGATC	1 cut(s)	257
AoxI	GGCC	2 cut(s)	177, 284
ApoI	RAATTY	1 cut(s)	236
AspS9I	GGNCC	2 cut(s)	46, 177
AsuHPI	GGTGA	2 cut(s)	68, 116
AsuII	TTCGAA	1 cut(s)	295
AvaII	GGWCC	1 cut(s)	46
BanI	GGYRCC	1 cut(s)	103
BarI	GAAGNNNNNNTAC	2 cut(s)	335, 367
BauI	CACGAG	1 cut(s)	213
BbsI	GAAGAC	1 cut(s)	146
BccI	CCATC	1 cut(s)	388
BclI	TGATCA	2 cut(s)	279, 337
BcoDI	GTCTC	1 cut(s)	18
Bme18I	GGWCC	1 cut(s)	46
BmgT120I	GGNCC	2 cut(s)	46, 177
BmiI	GGNNCC	1 cut(s)	105
BpiI	GAAGAC	1 cut(s)	146
Bpu14I	TTCGAA	1 cut(s)	295
BsaBI	GATNNNNATC	2 cut(s)	261, 336
Bse8I	GATNNNNATC	2 cut(s)	261, 336
BseGI	GGATG	1 cut(s)	73
BseJI	GATNNNNATC	2 cut(s)	261, 336
BseMII	CTCAG	2 cut(s)	164, 294
BseRI	GAGGAG	1 cut(s)	131
BshFI	GGCC	2 cut(s)	179, 286
BshNI	GGYRCC	1 cut(s)	103
BsmAI	GTCTC	1 cut(s)	18
BsmBI	CGTCTC	1 cut(s)	18
BsnI	GGCC	2 cut(s)	179, 286
Bsp119I	TTCGAA	1 cut(s)	295
Bsp143I	GATC	4 cut(s)	262, 279, 289, 337
BspANI	GGCC	2 cut(s)	179, 286
BspCNI	CTCAG	2 cut(s)	165, 295
BspLI	GGNNCC	1 cut(s)	105
BspPI	GGATC	1 cut(s)	257
BspT104I	TTCGAA	1 cut(s)	295
BspT107I	GGYRCC	1 cut(s)	103
BssMI	GATC	4 cut(s)	262, 279, 289, 337
BssSI	CACGAG	1 cut(s)	213
Bst2BI	CACGAG	1 cut(s)	213
Bst4CI	ACNGT	2 cut(s)	170, 222
BstBI	TTCGAA	1 cut(s)	295
BstDEI	CTNAG	2 cut(s)	173, 303
BstF5I	GGATG	1 cut(s)	73
BstKTI	GATC	4 cut(s)	265, 282, 292, 340
BstMAI	GTCTC	1 cut(s)	18
BstMBI	GATC	4 cut(s)	262, 279, 289, 337
BstMWI	GCNNNNNNNGC	1 cut(s)	381
BstNSI	RCATGY	1 cut(s)	138
BstV2I	GAAGAC	1 cut(s)	146
BsuRI	GGCC	2 cut(s)	179, 286
BtsCI	GGATG	1 cut(s)	73
Cfr13I	GGNCC	2 cut(s)	46, 177
Csp6I	GTAC	1 cut(s)	218
CviAII	CATG	1 cut(s)	135
CviJI	RGCY	7 cut(s)	84, 179, 197, 212, 286, 346, 410
CviKI_1	RGCY	7 cut(s)	84, 179, 197, 212, 286, 346, 410
CviQI	GTAC	1 cut(s)	218
DdeI	CTNAG	2 cut(s)	173, 303
DpnI	GATC	4 cut(s)	264, 281, 291, 339
DpnII	GATC	4 cut(s)	262, 279, 289, 337
Eco147I	AGGCCT	1 cut(s)	286
Eco47I	GGWCC	1 cut(s)	46
Esp3I	CGTCTC	1 cut(s)	18
FaeI	CATG	1 cut(s)	138
FaiI	YATR	5 cut(s)	136, 165, 254, 349, 369
FalI	AAGNNNNNCTT	4 cut(s)	299, 331, 349, 381
FatI	CATG	1 cut(s)	134
FbaI	TGATCA	2 cut(s)	279, 337
FokI	GGATG	1 cut(s)	80
HaeIII	GGCC	2 cut(s)	179, 286
Hin1II	CATG	1 cut(s)	138
HphI	GGTGA	2 cut(s)	68, 116
Hpy166II	GTNNAC	1 cut(s)	218
Hpy188I	TCNGA	3 cut(s)	67, 174, 342
Hpy188III	TCNNGA	3 cut(s)	227, 244, 266
Hpy8I	GTNNAC	1 cut(s)	218
HpyAV	CCTTC	2 cut(s)	53, 372
HpyCH4III	ACNGT	2 cut(s)	170, 222
HpyCH4IV	ACGT	1 cut(s)	11
HpyF10VI	GCNNNNNNNGC	1 cut(s)	381
HpyF3I	CTNAG	2 cut(s)	173, 303
HpySE526I	ACGT	1 cut(s)	11
Hsp92II	CATG	1 cut(s)	138
Ksp22I	TGATCA	2 cut(s)	279, 337
Kzo9I	GATC	4 cut(s)	262, 279, 289, 337
LmnI	GCTCC	1 cut(s)	381
LpnPI	CCDG	8 cut(s)	17, 39, 152, 268, 279, 300, 357, 396
MaeII	ACGT	1 cut(s)	11
MaeIII	GTNAC	1 cut(s)	403
MalI	GATC	4 cut(s)	264, 281, 291, 339
MboI	GATC	4 cut(s)	262, 279, 289, 337
MboII	GAAGA	4 cut(s)	146, 154, 284, 309
MluCI	AATT	2 cut(s)	21, 236
MnlI	CCTC	3 cut(s)	109, 168, 202
MseI	TTAA	2 cut(s)	24, 147
MslI	CAYNNNNRTG	1 cut(s)	162
MspA1I	CMGCKG	1 cut(s)	410
MwoI	GCNNNNNNNGC	1 cut(s)	381
NdeII	GATC	4 cut(s)	262, 279, 289, 337
NlaIII	CATG	1 cut(s)	138
NlaIV	GGNNCC	1 cut(s)	105
NspI	RCATGY	1 cut(s)	138
NspV	TTCGAA	1 cut(s)	295
PceI	AGGCCT	1 cut(s)	286
PspN4I	GGNNCC	1 cut(s)	105
PspPI	GGNCC	2 cut(s)	46, 177
PvuII	CAGCTG	1 cut(s)	410
RsaI	GTAC	1 cut(s)	219
RsaNI	GTAC	1 cut(s)	218
RseI	CAYNNNNRTG	1 cut(s)	162
SaqAI	TTAA	2 cut(s)	24, 147
Sau3AI	GATC	4 cut(s)	262, 279, 289, 337
Sau96I	GGNCC	2 cut(s)	46, 177
SetI	ASST	5 cut(s)	14, 64, 154, 199, 412
SfuI	TTCGAA	1 cut(s)	295
SinI	GGWCC	1 cut(s)	46
SmiMI	CAYNNNNRTG	1 cut(s)	162
Sse9I	AATT	2 cut(s)	21, 236
SseBI	AGGCCT	1 cut(s)	286
StuI	AGGCCT	1 cut(s)	286
TaaI	ACNGT	2 cut(s)	170, 222
TaiI	ACGT	1 cut(s)	14
TaqI	TCGA	2 cut(s)	188, 295
TasI	AATT	2 cut(s)	21, 236
Tru1I	TTAA	2 cut(s)	24, 147
Tru9I	TTAA	2 cut(s)	24, 147
TspDTI	ATGAA	2 cut(s)	84, 146
VpaK11BI	GGWCC	1 cut(s)	46
XapI	RAATTY	1 cut(s)	236
XceI	RCATGY	1 cut(s)	138

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.