Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0000473

Physical Location & Seq

Genomic Coordinates Reverse (-)

24011207 .. 24014472

Gene Structure 3266 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G018500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1056 bp

ATGTACCATTCAGTGTTCTTTTGTCCGGAGGATTGGACTCCACTCGTTGCTGCTGTGGCTTGTCACTATTTGGCTAAATCAGAAGCTGCATGTCAGTGGGGGTCACAATTACATACCTTTTGCGTTGGCTTGGAGGGTTCACCAGATTTAAAAGCAGCAAGAGAAGTTGCTGATTATCTTGGAACTCATCATCACGAGTTTCACTTTACTCTTCAGGTAATTTATAAGGGATGCATAGATGCACTTGAAGAAGTTATCTACCACATTGAGACATATGATGTGATCATTATTAAATCCCTGGGAGTAAAAATGGTCCTTTCTGGTGAAGGTTCAGATGCAATCTTTGGTGGCTACTTGTATTTCCACAAGGCACCAAACAAGGAGGAGTTTCACCAAGAAACATGCCAGAAGATTAAAGCTCTTCATCTGTACGACTGTCTGAGGGCCAACAAATCGACTTCAGCTTGGGGTGTTGAGGCTCGAGTACCGTTTCTTGACAAAGAATTCATCAAGACTGCTATGAACATTGATCCAGAATGGAAAATGATCAGGCCTGATCTTGGGAGAATTGAGAAGTGGGTCTTGCGCAAAGTATTTGATGATGATCAGAAGCCATATTTACCAAAGCACATCTTGTACAAGCAGAAGGAGCAGTTTAGTGATGGTGTCGGTTACAGCTGGATTGACGGCTTGAAAGATCATGCCAACAAACAAGTAACAGATTCAATGCTGACGAACGCAAGCTTTGTCTACCCTGAAAACTCTCCACCTACAAAAGAAGGATACTACTATAGGACTATCTTTGAGAAATATTTTCCAAAGAATGTTGCGAGATCGACAGTTCCTGGTGGTCCAAGTGTGGCATGCAGCACAGCAAAAGCTGTAGAGTGGGATGCCGAATGGTCCAAGAACCCTGATCCATCTGGTCGTGCTGCACTTGGTATACATGCAGCTTCATATGAGGAATCCAAAGATGTGAAGACTGGAAGTCTTGTGAGTGACTCTCCACAGAAACTTGTAAAAGGGGTTGCAGAGAAAGTGGCTGCAGTAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004066 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006082 GO:0006520 GO:0006528 GO:0006529 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009066 GO:0009067 GO:0009314 GO:0009416 GO:0009506 GO:0009628 GO:0009642 GO:0009646 GO:0009651 GO:0009987 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019752 GO:0030054 GO:0032787 GO:0034641 GO:0042538 GO:0042802 GO:0042803 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046983 GO:0050896 GO:0055044 GO:0071704 GO:0072330 GO:0097164 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00250 ko01100 ko01110 map00250 map01100 map01110

Pfam Domains

Protein Families

Asn_synthase GATase_7

Protein Analysis

351

Amino Acids

39.5

Weight (kDa)

6.17

Isoelectric Point (pI)

44.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asn_synthase	PF00733	17 - 127	1.1e-19	Asparagine synthase
Asn_synthase	PF00733	125 - 276	1.3e-31	Asparagine synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000473)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10240 AT5G10240 AT5G65010 AT5G65010
fragaria_vesca	FvH4_1g20910 FvH4_1g20910 FvH4_1g29202 FvH4_3g23760 FvH4_3g42550
malus_domestica	MD01G1029700.v1.1 MD15G1324200.v1.1
prunus_persica	Prupe.6G186800_v2.0.a1 Prupe.6G186800_v2.0.a1 Prupe.6G186800_v2.0.a1
pyrus_communis	pycom01g05790
rosa_chinensis	RchiOBHm_Chr2g0113551 RchiOBHm_Chr2g0113591 RchiOBHm_Chr2g0113641 RchiOBHm_Chr2g0113651 RchiOBHm_Chr2g0113711 RchiOBHm_Chr2g0113721 RchiOBHm_Chr2g0113831 RchiOBHm_Chr2g0113841 RchiOBHm_Chr2g0113851 RchiOBHm_Chr2g0113911 RchiOBHm_Chr2g0113941 RchiOBHm_Chr2g0113991 RchiOBHm_Chr2g0114001 RchiOBHm_Chr2g0114041 RchiOBHm_Chr2g0114051 RchiOBHm_Chr2g0114061 RchiOBHm_Chr2g0114071 RchiOBHm_Chr2g0114081 RchiOBHm_Chr2g0114101 RchiOBHm_Chr2g0114131 RchiOBHm_Chr2g0114141 RchiOBHm_Chr2g0114261 RchiOBHm_Chr2g0114271 RchiOBHm_Chr2g0114291 RchiOBHm_Chr2g0124041
rosa_laevigata	RLG00000018073 RLG00000018076 RLG00000018078 RLG00000018084 RLG00000018086 RLG00000018088 RLG00000018094 RLG00000018095
rosa_multiflora	Rmu_sc0000881.1_g000007 Rmu_sc0000929.1_g000008 Rmu_sc0000929.1_g000017 Rmu_sc0000929.1_g000029 Rmu_sc0000929.1_g000039 Rmu_sc0001699.1_g000005 Rmu_sc0004283.1_g000004 Rmu_sc0007010.1_g000001 Rmu_sc0007415.1_g000028 Rmu_sc0007415.1_g000045 Rmu_sc0009639.1_g000009 Rmu_sc0010543.1_g000008 Rmu_sc0011941.1_g000022 Rmu_sc0018769.1_g000001
rosa_roxburghii	Rroxscaffold_2G00129150 Rroxscaffold_2G00130050 Rroxscaffold_2G00130070 Rroxscaffold_2G00130090
rosa_rugosa	Rorug02G0188100 Rorug02G0188100 Rorug02G0189700 Rorug02G0190000 Rorug02G0190000 Rorug03G0301100
rosa_samantha	Rh2AG242700 Rh2AG243100 Rh2AG245300 Rh2AG246800 Rh2BG256600 Rh2DG253800 Rh6CG172600
rosa_wichuraiana	Rw0G014630 Rw2G018450 Rw2G018500 Rw2G018980 Rw2G019030 Rw2G019050 Rw2G019100 Rw2G019130 Rw2G019190 Rw2G019420 Rw5G028430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	225
Acc16I	TGCGCA	1 cut(s)	587
AccB1I	GGYRCC	1 cut(s)	370
AccI	GTMKAC	2 cut(s)	750, 943
AccIII	TCCGGA	1 cut(s)	25
AclWI	GGATC	2 cut(s)	524, 911
AcsI	RAATTY	1 cut(s)	503
AcuI	CTGAAG	2 cut(s)	197, 444
AfaI	GTAC	4 cut(s)	5, 431, 486, 638
AfiI	CCNNNNNNNGG	1 cut(s)	560
AgsI	TTSAA	3 cut(s)	248, 694, 726
AhdI	GACNNNNNGTC	1 cut(s)	987
AjnI	CCWGG	2 cut(s)	297, 844
AluBI	AGCT	7 cut(s)	86, 419, 464, 678, 744, 881, 953
AluI	AGCT	7 cut(s)	86, 419, 464, 678, 744, 881, 953
Alw26I	GTCTC	1 cut(s)	263
AlwI	GGATC	2 cut(s)	524, 911
AlwNI	CAGNNNCTG	2 cut(s)	86, 845
Ama87I	CYCGRG	1 cut(s)	480
Aor13HI	TCCGGA	1 cut(s)	25
AoxI	GGCC	2 cut(s)	444, 551
ApeKI	GCWGC	7 cut(s)	50, 86, 155, 867, 932, 950, 1043
ApoI	RAATTY	1 cut(s)	503
AspLEI	GCGC	1 cut(s)	588
AspS9I	GGNCC	4 cut(s)	313, 444, 851, 903
AsuHPI	GGTGA	3 cut(s)	132, 335, 383
AvaI	CYCGRG	1 cut(s)	480
AvaII	GGWCC	3 cut(s)	313, 851, 903
BanI	GGYRCC	1 cut(s)	370
BauI	CACGAG	1 cut(s)	194
BbsI	GAAGAC	1 cut(s)	986
BbvI	GCAGC	7 cut(s)	37, 73, 167, 879, 919, 962, 1030
BccI	CCATC	2 cut(s)	656, 928
BceAI	ACGGC	1 cut(s)	703
BciT130I	CCWGG	2 cut(s)	299, 846
BciVI	GTATCC	1 cut(s)	776
BclI	TGATCA	3 cut(s)	282, 546, 604
BcoDI	GTCTC	1 cut(s)	263
BfmI	CTRYAG	3 cut(s)	790, 882, 1044
BfuI	GTATCC	1 cut(s)	776
BisI	GCNGC	7 cut(s)	51, 87, 156, 868, 933, 951, 1044
BlsI	GCNGC	7 cut(s)	52, 88, 157, 869, 934, 952, 1045
Bme1390I	CCNGG	2 cut(s)	299, 846
Bme18I	GGWCC	3 cut(s)	313, 851, 903
BmeRI	GACNNNNNGTC	1 cut(s)	987
BmeT110I	CYCGRG	1 cut(s)	480
BmgT120I	GGNCC	4 cut(s)	313, 444, 851, 903
BmiI	GGNNCC	1 cut(s)	372
BmrFI	CCNGG	2 cut(s)	299, 846
BmsI	GCATC	4 cut(s)	221, 229, 325, 883
BpiI	GAAGAC	1 cut(s)	986
BplI	GAGNNNNNCTC	2 cut(s)	988, 1020
BsaBI	GATNNNNATC	1 cut(s)	603
BsaJI	CCNNGG	2 cut(s)	297, 298
BsaWI	WCCGGW	1 cut(s)	25
Bsc4I	CCNNNNNNNGG	1 cut(s)	560
Bse1I	ACTGG	1 cut(s)	988
Bse8I	GATNNNNATC	1 cut(s)	603
BseAI	TCCGGA	1 cut(s)	25
BseBI	CCWGG	2 cut(s)	299, 846
BseDI	CCNNGG	2 cut(s)	297, 298
BseGI	GGATG	2 cut(s)	236, 898
BseJI	GATNNNNATC	1 cut(s)	603
BseLI	CCNNNNNNNGG	1 cut(s)	560
BseMII	CTCAG	1 cut(s)	431
BseNI	ACTGG	1 cut(s)	988
BseRI	GAGGAG	1 cut(s)	398
BseXI	GCAGC	7 cut(s)	37, 73, 167, 879, 919, 962, 1030
BsgI	GTGCAG	1 cut(s)	918
BshFI	GGCC	2 cut(s)	446, 553
BshNI	GGYRCC	1 cut(s)	370
BsiHKCI	CYCGRG	1 cut(s)	480
BsiSI	CCGG	1 cut(s)	26
BslI	CCNNNNNNNGG	1 cut(s)	560
BsmAI	GTCTC	1 cut(s)	263
BsnI	GGCC	2 cut(s)	446, 553
BsoBI	CYCGRG	1 cut(s)	480
Bsp13I	TCCGGA	1 cut(s)	25
Bsp1407I	TGTACA	1 cut(s)	636
Bsp143I	GATC	8 cut(s)	282, 529, 546, 556, 604, 697, 833, 916
BspANI	GGCC	2 cut(s)	446, 553
BspCNI	CTCAG	1 cut(s)	432
BspEI	TCCGGA	1 cut(s)	25
BspLI	GGNNCC	1 cut(s)	372
BspMAI	CTGCAG	1 cut(s)	1048
BspPI	GGATC	2 cut(s)	524, 911
BspQI	GCTCTTC	1 cut(s)	426
BspT107I	GGYRCC	1 cut(s)	370
BsrGI	TGTACA	1 cut(s)	636
BsrI	ACTGG	1 cut(s)	988
BssECI	CCNNGG	2 cut(s)	297, 298
BssMI	GATC	8 cut(s)	282, 529, 546, 556, 604, 697, 833, 916
BssNAI	GTATAC	1 cut(s)	944
BssSI	CACGAG	1 cut(s)	194
Bst1107I	GTATAC	1 cut(s)	944
Bst2BI	CACGAG	1 cut(s)	194
Bst2UI	CCWGG	2 cut(s)	299, 846
Bst4CI	ACNGT	3 cut(s)	437, 489, 841
Bst6I	CTCTTC	2 cut(s)	216, 426
BstAUI	TGTACA	1 cut(s)	636
BstC8I	GCNNGC	2 cut(s)	742, 865
BstDEI	CTNAG	1 cut(s)	440
BstF5I	GGATG	2 cut(s)	236, 898
BstHHI	GCGC	1 cut(s)	588
BstKTI	GATC	8 cut(s)	285, 532, 549, 559, 607, 700, 836, 919
BstMAI	GTCTC	1 cut(s)	263
BstMBI	GATC	8 cut(s)	282, 529, 546, 556, 604, 697, 833, 916
BstMWI	GCNNNNNNNGC	2 cut(s)	56, 649
BstNI	CCWGG	2 cut(s)	299, 846
BstNSI	RCATGY	4 cut(s)	93, 405, 867, 950
BstSCI	CCNGG	2 cut(s)	297, 844
BstSFI	CTRYAG	3 cut(s)	790, 882, 1044
BstV1I	GCAGC	7 cut(s)	37, 73, 167, 879, 919, 962, 1030
BstV2I	GAAGAC	1 cut(s)	986
BstZ17I	GTATAC	1 cut(s)	944
BsuI	GTATCC	1 cut(s)	776
BsuRI	GGCC	2 cut(s)	446, 553
BtsCI	GGATG	2 cut(s)	236, 898
BtsIMutI	CAGTG	2 cut(s)	18, 101
Cac8I	GCNNGC	2 cut(s)	742, 865
CaiI	CAGNNNCTG	2 cut(s)	86, 845
CfoI	GCGC	1 cut(s)	588
Cfr13I	GGNCC	4 cut(s)	313, 444, 851, 903
Csp6I	GTAC	4 cut(s)	4, 430, 485, 637
CviAII	CATG	5 cut(s)	90, 402, 701, 864, 947
CviQI	GTAC	4 cut(s)	4, 430, 485, 637
DdeI	CTNAG	1 cut(s)	440
DpnI	GATC	8 cut(s)	284, 531, 548, 558, 606, 699, 835, 918
DpnII	GATC	8 cut(s)	282, 529, 546, 556, 604, 697, 833, 916
DraI	TTTAAA	1 cut(s)	150
DriI	GACNNNNNGTC	1 cut(s)	987
Eam1104I	CTCTTC	2 cut(s)	216, 426
Eam1105I	GACNNNNNGTC	1 cut(s)	987
EarI	CTCTTC	2 cut(s)	216, 426
Eco147I	AGGCCT	1 cut(s)	553
Eco47I	GGWCC	3 cut(s)	313, 851, 903
Eco57I	CTGAAG	2 cut(s)	197, 444
Eco88I	CYCGRG	1 cut(s)	480
EcoRI	GAATTC	1 cut(s)	503
EcoRII	CCWGG	2 cut(s)	297, 844
EcoT22I	ATGCAT	1 cut(s)	236
FaeI	CATG	5 cut(s)	93, 405, 704, 867, 950
FalI	AAGNNNNNCTT	4 cut(s)	566, 598, 617, 649
FatI	CATG	5 cut(s)	89, 401, 700, 863, 946
FauNDI	CATATG	2 cut(s)	274, 958
FbaI	TGATCA	3 cut(s)	282, 546, 604
FblI	GTMKAC	2 cut(s)	750, 943
Fnu4HI	GCNGC	7 cut(s)	51, 87, 156, 868, 933, 951, 1044
FokI	GGATG	2 cut(s)	243, 905
Fsp4HI	GCNGC	7 cut(s)	51, 87, 156, 868, 933, 951, 1044
FspI	TGCGCA	1 cut(s)	587
GlaI	GCGC	1 cut(s)	587
GluI	GCNGC	7 cut(s)	51, 87, 156, 868, 933, 951, 1044
HaeIII	GGCC	2 cut(s)	446, 553
HapII	CCGG	1 cut(s)	26
HhaI	GCGC	1 cut(s)	588
Hin1II	CATG	5 cut(s)	93, 405, 704, 867, 950
Hin6I	GCGC	1 cut(s)	586
HinP1I	GCGC	1 cut(s)	586
HindIII	AAGCTT	1 cut(s)	742
HinfI	GANTC	4 cut(s)	37, 722, 965, 1001
HpaII	CCGG	1 cut(s)	26
HphI	GGTGA	3 cut(s)	132, 335, 383
Hpy166II	GTNNAC	3 cut(s)	140, 751, 944
Hpy188I	TCNGA	4 cut(s)	82, 334, 441, 609
Hpy188III	TCNNGA	5 cut(s)	26, 194, 494, 511, 533
Hpy8I	GTNNAC	3 cut(s)	140, 751, 944
HpyAV	CCTTC	3 cut(s)	320, 640, 773
HpyCH4III	ACNGT	3 cut(s)	437, 489, 841
HpyCH4V	TGCA	9 cut(s)	89, 234, 242, 338, 867, 935, 950, 1031, 1046
HpyF10VI	GCNNNNNNNGC	2 cut(s)	56, 649
HpyF3I	CTNAG	1 cut(s)	440
Hsp92II	CATG	5 cut(s)	93, 405, 704, 867, 950
HspAI	GCGC	1 cut(s)	586
Kpn2I	TCCGGA	1 cut(s)	25
Ksp22I	TGATCA	3 cut(s)	282, 546, 604
Kzo9I	GATC	8 cut(s)	282, 529, 546, 556, 604, 697, 833, 916
LguI	GCTCTTC	1 cut(s)	426
LmnI	GCTCC	1 cut(s)	649
Lsp1109I	GCAGC	7 cut(s)	37, 73, 167, 879, 919, 962, 1030
LweI	GCATC	4 cut(s)	221, 229, 325, 883
MaeIII	GTNAC	5 cut(s)	62, 102, 671, 715, 998
MalI	GATC	8 cut(s)	284, 531, 548, 558, 606, 699, 835, 918
MboI	GATC	8 cut(s)	282, 529, 546, 556, 604, 697, 833, 916
MboII	GAAGA	5 cut(s)	203, 260, 413, 421, 991
MluCI	AATT	4 cut(s)	107, 219, 503, 567
MlyI	GAGTC	2 cut(s)	31, 995
MnlI	CCTC	6 cut(s)	22, 127, 376, 435, 469, 955
Mph1103I	ATGCAT	1 cut(s)	236
MroI	TCCGGA	1 cut(s)	25
MseI	TTAA	3 cut(s)	149, 291, 414
MslI	CAYNNNNRTG	1 cut(s)	94
MspA1I	CMGCKG	1 cut(s)	678
MspI	CCGG	1 cut(s)	26
MspR9I	CCNGG	2 cut(s)	299, 846
MvaI	CCWGG	2 cut(s)	299, 846
MwoI	GCNNNNNNNGC	2 cut(s)	56, 649
NdeI	CATATG	2 cut(s)	274, 958
NdeII	GATC	8 cut(s)	282, 529, 546, 556, 604, 697, 833, 916
NlaIII	CATG	5 cut(s)	93, 405, 704, 867, 950
NlaIV	GGNNCC	1 cut(s)	372
NmuCI	GTSAC	3 cut(s)	62, 102, 998
NsbI	TGCGCA	1 cut(s)	587
NsiI	ATGCAT	1 cut(s)	236
NspI	RCATGY	4 cut(s)	93, 405, 867, 950
PaeI	GCATGC	1 cut(s)	867
PaeR7I	CTCGAG	1 cut(s)	480
PasI	CCCWGGG	1 cut(s)	298
PceI	AGGCCT	1 cut(s)	553
PciSI	GCTCTTC	1 cut(s)	426
PfeI	GAWTC	2 cut(s)	722, 965
PkrI	GCNGC	7 cut(s)	52, 88, 157, 869, 934, 952, 1045
PleI	GAGTC	2 cut(s)	31, 995
PpsI	GAGTC	2 cut(s)	31, 995
PsiI	TTATAA	1 cut(s)	225
Psp6I	CCWGG	2 cut(s)	297, 844
PspGI	CCWGG	2 cut(s)	297, 844
PspN4I	GGNNCC	1 cut(s)	372
PspPI	GGNCC	4 cut(s)	313, 444, 851, 903
PspXI	VCTCGAGB	1 cut(s)	480
PstI	CTGCAG	1 cut(s)	1048
PstNI	CAGNNNCTG	2 cut(s)	86, 845
PvuII	CAGCTG	1 cut(s)	678
RsaI	GTAC	4 cut(s)	5, 431, 486, 638
RsaNI	GTAC	4 cut(s)	4, 430, 485, 637
RseI	CAYNNNNRTG	1 cut(s)	94
SapI	GCTCTTC	1 cut(s)	426
SaqAI	TTAA	3 cut(s)	149, 291, 414
SatI	GCNGC	7 cut(s)	51, 87, 156, 868, 933, 951, 1044
Sau3AI	GATC	8 cut(s)	282, 529, 546, 556, 604, 697, 833, 916
Sau96I	GGNCC	4 cut(s)	313, 444, 851, 903
SchI	GAGTC	2 cut(s)	31, 995
ScrFI	CCNGG	2 cut(s)	299, 846
SfaNI	GCATC	4 cut(s)	221, 229, 325, 883
SfcI	CTRYAG	3 cut(s)	790, 882, 1044
Sfr274I	CTCGAG	1 cut(s)	480
SinI	GGWCC	3 cut(s)	313, 851, 903
SlaI	CTCGAG	1 cut(s)	480
SmiMI	CAYNNNNRTG	1 cut(s)	94
SmlI	CTYRAG	1 cut(s)	480
SmoI	CTYRAG	1 cut(s)	480
SphI	GCATGC	1 cut(s)	867
Sse9I	AATT	4 cut(s)	107, 219, 503, 567
SseBI	AGGCCT	1 cut(s)	553
SspI	AATATT	1 cut(s)	812
StuI	AGGCCT	1 cut(s)	553
StyD4I	CCNGG	2 cut(s)	297, 844
TaaI	ACNGT	3 cut(s)	437, 489, 841
TaqI	TCGA	3 cut(s)	455, 481, 836
TasI	AATT	4 cut(s)	107, 219, 503, 567
TatI	WGTACW	1 cut(s)	636
TfiI	GAWTC	2 cut(s)	722, 965
Tru1I	TTAA	3 cut(s)	149, 291, 414
Tru9I	TTAA	3 cut(s)	149, 291, 414
TscAI	CASTG	2 cut(s)	18, 101
TseFI	GTSAC	3 cut(s)	62, 102, 998
TseI	GCWGC	7 cut(s)	50, 86, 155, 867, 932, 950, 1043
Tsp45I	GTSAC	3 cut(s)	62, 102, 998
TspDTI	ATGAA	4 cut(s)	413, 496, 536, 945
TspRI	CASTG	2 cut(s)	18, 101
VpaK11BI	GGWCC	3 cut(s)	313, 851, 903
XapI	RAATTY	1 cut(s)	503
XceI	RCATGY	4 cut(s)	93, 405, 867, 950
XhoI	CTCGAG	1 cut(s)	480
XmiI	GTMKAC	2 cut(s)	750, 943
Zsp2I	ATGCAT	1 cut(s)	236

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.