Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0000473

Physical Location & Seq

Genomic Coordinates Forward (+)

13026790 .. 13034280

Gene Structure 7491 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g20910.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1911 bp

ATGGCAACAGCAACGCCCCCAGCTTTATATATTACAAATTCCCCGAAGATTCCTTTTCCGCAGCCTCTACTTTTGAAAATCTGCCACTCAATTTTAGACTCTCGTTTAGAAAGAAGCTACAGTCCTCCTCTCCGCCGCCGCGACGTCGCCGCCATGTGTGGAATTCTCGCCGTCTTGGGATGCGTGGACCACTCCCAGGCCAAGCGCAGCCGCATTATCGAGCTCTCCCGCCGGTTGAGGCATCGCGGTCCTGATTGGAGCGGCTTGCATTGCCATGGAGATTGCTACCTTGCGCATCAGCGATTGGCTATCGTGGACCCTGCCTCAGGTGATCAGCCACTCTACAACGAGGACAAGACCGTCGTTGTCACGGTTAACGGTGAGATATATAACCACAAGGAATTGAGAGAAACATTGAAGGGTCATCAATTCCGAACTGGTAGTGATTGTGAAGTGATAACGCATCTTTATGAAGAACATGGGGAAGAATTTGTTGACATGCTGGACGGGATGTTCTCCTTTGTCCTCCTTGATACAAAAGACAATAGTTTCATGGCGGCTCGTGATGCTATTGGCATAACCCCACTGTACATGGGCTGGGGTCCTGATGGATCAATTTGGTTTGCTTCAGAAATGAAAGCTCTAAGTGATGATTGTGAAAGATTCATAACTTTTCCTCCTGGGCATCTATACTCTAGCAAACAAGGAGGGCTGAGAAGGTGGTACAATCCACAATGGTTTTCAGAGAAGATACCATCGACTCCTTATGATCCCATTGTTTTACGCAGGGCTTTTGAGAAGGCTGTGTTGAAGAGACTTATGACAGATGTACCATTCGGTGTTCTTTTGTCTGGAGGGTTGGACTCATCACTCGTTGCTGCTGTGGCTTCTCGCTATTTGGCTACATCAGAAGCTGCACGTCAGTGGGGATCACAGTTACATACCTTTTGCGTTGGCTTGGAGGGTTCTCCAGATTTGAAAGCAGCAAGAGAAGTTGCAGATTATCTTGGAACTCATCATCACGAGTTTCACTTTACTGTTCAGGAAGGCATAGACGCACTTGAAGAAGTTATCTACCATATTGAGACATATGATGTGACCTCTATCAGAGCCAGCACTCCAATGTTTCTCATGTCTCGTAAGATTAAATCTCTGGGAGTAAAAATGGTCCTCTCTGGTGAAGGTTCAGATGAAATCTTTGGTGGTTACTTGTATTTCCACAAGGCACCAAGCAAGGAGGAGTTTCACCAAGAGACATGCCAGAAGATCAAAGCTCTTCATCTGTACGACTGTCTGAGGGCCAACAAGTCGACTTCAGCTTGGGGTGTTGAGGCTCGTGTACCGTTTCTTGACAAAGACTTCATCAAGACTGCTATGGACATTGATCCAGAATGGAAAATGATCAGGCCTGATCTTGGGAGGATCGAGAAGTGGGTCTTACGCAAAGCATTTGATGATGAAGAGAAGCCATATTTACCAAAGCACATATTGTACAGGCAGAAGGAGCAGTTTAGTGATGGTGTTGGTTACAGCTGGATTGACGGCTTGAAAGATCATGCCAACAAACAAGTATCAGATTCAATGATGATGAATGCAGGCTTTATCTACCCTGAAAACACTCCAACCACAAAAGAAGGATACTACTATAGGACTATCTTTGAGAAATTTTTTCCAAAGAATTCTGCGAGATCGACAGTTCCTGGTGGTCCAAGTGTGGCATGCAGCACAGCAAAAGCTGTGGAGTGGGATGCAGAATGGTCCAAGAACCCTGATCCATCTGGTCGTGCTGCCCTTGGTATACATGCAGCTGCCTATGAGGAGTCAAAAGATGTGAAGACTGGAAGTCTTGTGAGTGACTCTCCACAGAAACTTGTAGAAGGGGTTGCAGGGAAAGTGGTGGGAGTAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004066 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006082 GO:0006520 GO:0006528 GO:0006529 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009066 GO:0009067 GO:0009314 GO:0009416 GO:0009506 GO:0009628 GO:0009642 GO:0009646 GO:0009651 GO:0009987 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019752 GO:0030054 GO:0032787 GO:0034641 GO:0042538 GO:0042802 GO:0042803 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046983 GO:0050896 GO:0055044 GO:0071704 GO:0072330 GO:0097164 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00250 ko01100 ko01110 map00250 map01100 map01110

Pfam Domains

Protein Families

Asn_synthase GATase_7

Protein Analysis

637

Amino Acids

71.46

Weight (kDa)

6.3

Isoelectric Point (pI)

45.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_6	PF13522	84 - 211	1.5e-36	Glutamine amidotransferase domain
GATase_7	PF13537	99 - 216	2.1e-44	Glutamine amidotransferase domain
DUF3700	PF12481	163 - 246	7.2e-12	Aluminium induced protein
Asn_synthase	PF00733	261 - 412	8.3e-53	Asparagine synthase
Asn_synthase	PF00733	410 - 562	4.6e-33	Asparagine synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0000473)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10240 AT5G10240 AT5G65010 AT5G65010
fragaria_vesca	FvH4_1g20910 FvH4_1g20910 FvH4_1g29202 FvH4_3g23760 FvH4_3g42550
malus_domestica	MD01G1029700.v1.1 MD15G1324200.v1.1
prunus_persica	Prupe.6G186800_v2.0.a1 Prupe.6G186800_v2.0.a1 Prupe.6G186800_v2.0.a1
pyrus_communis	pycom01g05790
rosa_chinensis	RchiOBHm_Chr2g0113551 RchiOBHm_Chr2g0113591 RchiOBHm_Chr2g0113641 RchiOBHm_Chr2g0113651 RchiOBHm_Chr2g0113711 RchiOBHm_Chr2g0113721 RchiOBHm_Chr2g0113831 RchiOBHm_Chr2g0113841 RchiOBHm_Chr2g0113851 RchiOBHm_Chr2g0113911 RchiOBHm_Chr2g0113941 RchiOBHm_Chr2g0113991 RchiOBHm_Chr2g0114001 RchiOBHm_Chr2g0114041 RchiOBHm_Chr2g0114051 RchiOBHm_Chr2g0114061 RchiOBHm_Chr2g0114071 RchiOBHm_Chr2g0114081 RchiOBHm_Chr2g0114101 RchiOBHm_Chr2g0114131 RchiOBHm_Chr2g0114141 RchiOBHm_Chr2g0114261 RchiOBHm_Chr2g0114271 RchiOBHm_Chr2g0114291 RchiOBHm_Chr2g0124041
rosa_laevigata	RLG00000018073 RLG00000018076 RLG00000018078 RLG00000018084 RLG00000018086 RLG00000018088 RLG00000018094 RLG00000018095
rosa_multiflora	Rmu_sc0000881.1_g000007 Rmu_sc0000929.1_g000008 Rmu_sc0000929.1_g000017 Rmu_sc0000929.1_g000029 Rmu_sc0000929.1_g000039 Rmu_sc0001699.1_g000005 Rmu_sc0004283.1_g000004 Rmu_sc0007010.1_g000001 Rmu_sc0007415.1_g000028 Rmu_sc0007415.1_g000045 Rmu_sc0009639.1_g000009 Rmu_sc0010543.1_g000008 Rmu_sc0011941.1_g000022 Rmu_sc0018769.1_g000001
rosa_roxburghii	Rroxscaffold_2G00129150 Rroxscaffold_2G00130050 Rroxscaffold_2G00130070 Rroxscaffold_2G00130090
rosa_rugosa	Rorug02G0188100 Rorug02G0188100 Rorug02G0189700 Rorug02G0190000 Rorug02G0190000 Rorug03G0301100
rosa_samantha	Rh2AG242700 Rh2AG243100 Rh2AG245300 Rh2AG246800 Rh2BG256600 Rh2DG253800 Rh6CG172600
rosa_wichuraiana	Rw0G014630 Rw2G018450 Rw2G018500 Rw2G018980 Rw2G019030 Rw2G019050 Rw2G019100 Rw2G019130 Rw2G019190 Rw2G019420 Rw5G028430

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	359
AatII	GACGTC	1 cut(s)	147
Acc16I	TGCGCA	1 cut(s)	294
AccB1I	GGYRCC	1 cut(s)	1225
AccBSI	CCGCTC	1 cut(s)	261
AccI	GTMKAC	2 cut(s)	1310, 1798
AccII	CGCG	2 cut(s)	141, 246
AclWI	GGATC	6 cut(s)	619, 764, 937, 1379, 1430, 1766
AcsI	RAATTY	5 cut(s)	37, 162, 488, 1664, 1678
AcuI	CTGAAG	2 cut(s)	612, 1299
AcyI	GRCGYC	1 cut(s)	144
AfaI	GTAC	6 cut(s)	590, 725, 831, 1286, 1341, 1493
AfiI	CCNNNNNNNGG	3 cut(s)	196, 326, 1415
AgsI	TTSAA	7 cut(s)	76, 418, 811, 979, 1064, 1549, 1581
AhdI	GACNNNNNGTC	1 cut(s)	1842
AjiI	CACGTC	1 cut(s)	920
AjnI	CCWGG	3 cut(s)	195, 679, 1699
AleI	CACNNNNGTG	1 cut(s)	922
AloI	GAACNNNNNNTCC	2 cut(s)	497, 529
Alw21I	GWGCWC	1 cut(s)	225
Alw26I	GTCTC	4 cut(s)	808, 1079, 1140, 1247
AlwI	GGATC	6 cut(s)	619, 764, 937, 1379, 1430, 1766
AlwNI	CAGNNNCTG	2 cut(s)	914, 1700
AoxI	GGCC	3 cut(s)	198, 1299, 1406
ApeKI	GCWGC	9 cut(s)	61, 207, 878, 914, 983, 1722, 1787, 1805, 1808
ApoI	RAATTY	5 cut(s)	37, 162, 488, 1664, 1678
AspLEI	GCGC	2 cut(s)	207, 295
AspS9I	GGNCC	8 cut(s)	187, 248, 316, 602, 1168, 1299, 1706, 1758
AsuHPI	GGTGA	4 cut(s)	341, 392, 1190, 1238
AvaII	GGWCC	7 cut(s)	187, 248, 316, 602, 1168, 1706, 1758
AxyI	CCTNAGG	1 cut(s)	325
BaeI	ACNNNNGTAYC	2 cut(s)	813, 846
BanI	GGYRCC	1 cut(s)	1225
BanII	GRGCYC	1 cut(s)	225
BauI	CACGAG	3 cut(s)	561, 1022, 1335
BbsI	GAAGAC	1 cut(s)	1841
Bbv12I	GWGCWC	1 cut(s)	225
BbvI	GCAGC	9 cut(s)	73, 219, 865, 901, 995, 1734, 1774, 1795, 1817
BccI	CCATC	4 cut(s)	602, 763, 1511, 1783
BceAI	ACGGC	2 cut(s)	155, 1558
BciT130I	CCWGG	3 cut(s)	197, 681, 1701
BciVI	GTATCC	1 cut(s)	1631
BclI	TGATCA	2 cut(s)	331, 1401
BcoDI	GTCTC	4 cut(s)	808, 1079, 1140, 1247
BfaI	CTAG	1 cut(s)	696
BfmI	CTRYAG	2 cut(s)	118, 1645
BfuI	GTATCC	1 cut(s)	1631
Bme1390I	CCNGG	3 cut(s)	197, 681, 1701
Bme18I	GGWCC	7 cut(s)	187, 248, 316, 602, 1168, 1706, 1758
BmeRI	GACNNNNNGTC	1 cut(s)	1842
BmgBI	CACGTC	1 cut(s)	920
BmgT120I	GGNCC	8 cut(s)	187, 248, 316, 602, 1168, 1299, 1706, 1758
BmiI	GGNNCC	3 cut(s)	318, 603, 1227
BmrFI	CCNGG	3 cut(s)	197, 681, 1701
BmsI	GCATC	7 cut(s)	170, 250, 304, 472, 556, 694, 1738
BpiI	GAAGAC	1 cut(s)	1841
BplI	GAGNNNNNCTC	2 cut(s)	1843, 1875
BpmI	CTGGAG	2 cut(s)	873, 954
BsaHI	GRCGYC	1 cut(s)	144
BsaJI	CCNNGG	4 cut(s)	195, 274, 680, 1792
BsaXI	ACNNNNNCTCC	2 cut(s)	661, 691
Bsc4I	CCNNNNNNNGG	3 cut(s)	196, 326, 1415
Bse118I	RCCGGY	1 cut(s)	231
Bse1I	ACTGG	2 cut(s)	442, 1843
Bse21I	CCTNAGG	1 cut(s)	325
Bse3DI	GCAATG	1 cut(s)	268
BseBI	CCWGG	3 cut(s)	197, 681, 1701
BseDI	CCNNGG	4 cut(s)	195, 274, 680, 1792
BseGI	GGATG	3 cut(s)	185, 516, 1753
BseLI	CCNNNNNNNGG	3 cut(s)	196, 326, 1415
BseMI	GCAATG	1 cut(s)	268
BseMII	CTCAG	3 cut(s)	339, 704, 1286
BseNI	ACTGG	2 cut(s)	442, 1843
BseRI	GAGGAG	3 cut(s)	117, 1253, 1832
BseXI	GCAGC	9 cut(s)	73, 219, 865, 901, 995, 1734, 1774, 1795, 1817
BseYI	CCCAGC	2 cut(s)	19, 597
BsgI	GTGCAG	1 cut(s)	900
Bsh1236I	CGCG	2 cut(s)	141, 246
BshFI	GGCC	3 cut(s)	200, 1301, 1408
BshNI	GGYRCC	1 cut(s)	1225
BsiHKAI	GWGCWC	1 cut(s)	225
BsiSI	CCGG	1 cut(s)	232
BslI	CCNNNNNNNGG	3 cut(s)	196, 326, 1415
BsmAI	GTCTC	4 cut(s)	808, 1079, 1140, 1247
BsmI	GAATGC	1 cut(s)	1597
BsnI	GGCC	3 cut(s)	200, 1301, 1408
Bsp1286I	GDGCHC	1 cut(s)	225
Bsp1407I	TGTACA	2 cut(s)	588, 1491
Bsp19I	CCATGG	1 cut(s)	274
BspANI	GGCC	3 cut(s)	200, 1301, 1408
BspCNI	CTCAG	3 cut(s)	338, 705, 1287
BspFNI	CGCG	2 cut(s)	141, 246
BspLI	GGNNCC	3 cut(s)	318, 603, 1227
BspPI	GGATC	6 cut(s)	619, 764, 937, 1379, 1430, 1766
BspQI	GCTCTTC	1 cut(s)	1281
BspT107I	GGYRCC	1 cut(s)	1225
BsrBI	CCGCTC	1 cut(s)	261
BsrDI	GCAATG	1 cut(s)	268
BsrFI	RCCGGY	1 cut(s)	231
BsrGI	TGTACA	2 cut(s)	588, 1491
BsrI	ACTGG	2 cut(s)	442, 1843
BssAI	RCCGGY	1 cut(s)	231
BssECI	CCNNGG	4 cut(s)	195, 274, 680, 1792
BssNAI	GTATAC	1 cut(s)	1799
BssNI	GRCGYC	1 cut(s)	144
BssSI	CACGAG	3 cut(s)	561, 1022, 1335
BssT1I	CCWWGG	2 cut(s)	274, 1792
Bst1107I	GTATAC	1 cut(s)	1799
Bst2BI	CACGAG	3 cut(s)	561, 1022, 1335
Bst2UI	CCWGG	3 cut(s)	197, 681, 1701
Bst6I	CTCTTC	3 cut(s)	806, 1281, 1455
BstACI	GRCGYC	1 cut(s)	144
BstAUI	TGTACA	2 cut(s)	588, 1491
BstC8I	GCNNGC	4 cut(s)	266, 1114, 1597, 1720
BstDEI	CTNAG	4 cut(s)	325, 644, 713, 1295
BstDSI	CCRYGG	1 cut(s)	274
BstENI	CCTNNNNNAGG	1 cut(s)	324
BstF5I	GGATG	3 cut(s)	185, 516, 1753
BstFNI	CGCG	2 cut(s)	141, 246
BstHHI	GCGC	2 cut(s)	207, 295
BstMAI	GTCTC	4 cut(s)	808, 1079, 1140, 1247
BstMWI	GCNNNNNNNGC	4 cut(s)	270, 566, 884, 1504
BstNI	CCWGG	3 cut(s)	197, 681, 1701
BstNSI	RCATGY	4 cut(s)	502, 1260, 1722, 1805
BstSCI	CCNGG	3 cut(s)	195, 679, 1699
BstSFI	CTRYAG	2 cut(s)	118, 1645
BstUI	CGCG	2 cut(s)	141, 246
BstV1I	GCAGC	9 cut(s)	73, 219, 865, 901, 995, 1734, 1774, 1795, 1817
BstV2I	GAAGAC	1 cut(s)	1841
BstZ17I	GTATAC	1 cut(s)	1799
Bsu36I	CCTNAGG	1 cut(s)	325
BsuI	GTATCC	1 cut(s)	1631
BsuRI	GGCC	3 cut(s)	200, 1301, 1408
BtgI	CCRYGG	1 cut(s)	274
BtgZI	GCGATG	1 cut(s)	227
BtrI	CACGTC	1 cut(s)	920
BtsCI	GGATG	3 cut(s)	185, 516, 1753
BtsIMutI	CAGTG	2 cut(s)	584, 929
Cac8I	GCNNGC	4 cut(s)	266, 1114, 1597, 1720
CaiI	CAGNNNCTG	2 cut(s)	914, 1700
CfoI	GCGC	2 cut(s)	207, 295
Cfr10I	RCCGGY	1 cut(s)	231
Cfr13I	GGNCC	8 cut(s)	187, 248, 316, 602, 1168, 1299, 1706, 1758
CseI	GACGC	1 cut(s)	1064
Csp6I	GTAC	6 cut(s)	589, 724, 830, 1285, 1340, 1492
CspCI	CAANNNNNGTGG	2 cut(s)	1719, 1754
CviQI	GTAC	6 cut(s)	589, 724, 830, 1285, 1340, 1492
DdeI	CTNAG	4 cut(s)	325, 644, 713, 1295
DrdI	GACNNNNNNGTC	1 cut(s)	359
DriI	GACNNNNNGTC	1 cut(s)	1842
DseDI	GACNNNNNNGTC	1 cut(s)	359
Eam1104I	CTCTTC	3 cut(s)	806, 1281, 1455
Eam1105I	GACNNNNNGTC	1 cut(s)	1842
EarI	CTCTTC	3 cut(s)	806, 1281, 1455
EciI	GGCGGA	1 cut(s)	122
Ecl136II	GAGCTC	1 cut(s)	223
Eco130I	CCWWGG	2 cut(s)	274, 1792
Eco147I	AGGCCT	1 cut(s)	1408
Eco24I	GRGCYC	1 cut(s)	225
Eco47I	GGWCC	7 cut(s)	187, 248, 316, 602, 1168, 1706, 1758
Eco53kI	GAGCTC	1 cut(s)	223
Eco57I	CTGAAG	2 cut(s)	612, 1299
Eco81I	CCTNAGG	1 cut(s)	325
EcoICRI	GAGCTC	1 cut(s)	223
EcoNI	CCTNNNNNAGG	1 cut(s)	324
EcoO109I	RGGNCCY	1 cut(s)	602
EcoRI	GAATTC	2 cut(s)	162, 1678
EcoRII	CCWGG	3 cut(s)	195, 679, 1699
EcoT14I	CCWWGG	2 cut(s)	274, 1792
EcoT38I	GRGCYC	1 cut(s)	225
ErhI	CCWWGG	2 cut(s)	274, 1792
FalI	AAGNNNNNCTT	2 cut(s)	1421, 1453
FauI	CCCGC	1 cut(s)	236
FauNDI	CATATG	1 cut(s)	1090
FbaI	TGATCA	2 cut(s)	331, 1401
FblI	GTMKAC	2 cut(s)	1310, 1798
FokI	GGATG	3 cut(s)	192, 523, 1760
FriOI	GRGCYC	1 cut(s)	225
FspBI	CTAG	1 cut(s)	696
FspI	TGCGCA	1 cut(s)	294
GlaI	GCGC	2 cut(s)	206, 294
GsaI	CCCAGC	2 cut(s)	23, 601
GsuI	CTGGAG	2 cut(s)	873, 954
HaeIII	GGCC	3 cut(s)	200, 1301, 1408
HapII	CCGG	1 cut(s)	232
HgaI	GACGC	1 cut(s)	1064
HhaI	GCGC	2 cut(s)	207, 295
Hin1I	GRCGYC	1 cut(s)	144
Hin6I	GCGC	2 cut(s)	205, 293
HinP1I	GCGC	2 cut(s)	205, 293
HincII	GTYRAC	3 cut(s)	376, 496, 1311
HindII	GTYRAC	3 cut(s)	376, 496, 1311
HinfI	GANTC	8 cut(s)	49, 98, 663, 760, 863, 1577, 1820, 1856
HpaI	GTTAAC	1 cut(s)	376
HpaII	CCGG	1 cut(s)	232
HphI	GGTGA	4 cut(s)	341, 392, 1190, 1238
Hpy166II	GTNNAC	7 cut(s)	187, 316, 376, 496, 1311, 1340, 1799
Hpy188I	TCNGA	8 cut(s)	434, 631, 745, 910, 1109, 1189, 1296, 1576
Hpy8I	GTNNAC	7 cut(s)	187, 316, 376, 496, 1311, 1340, 1799
Hpy99I	CGWCG	3 cut(s)	146, 149, 365
HpyAV	CCTTC	8 cut(s)	412, 711, 793, 1040, 1175, 1495, 1628, 1871
HpyCH4IV	ACGT	2 cut(s)	144, 919
HpyCH4V	TGCA	8 cut(s)	268, 917, 998, 1595, 1722, 1751, 1805, 1886
HpyF10VI	GCNNNNNNNGC	4 cut(s)	270, 566, 884, 1504
HpyF3I	CTNAG	4 cut(s)	325, 644, 713, 1295
HpySE526I	ACGT	2 cut(s)	144, 919
Hsp92I	GRCGYC	1 cut(s)	144
HspAI	GCGC	2 cut(s)	205, 293
Ksp22I	TGATCA	2 cut(s)	331, 1401
KspAI	GTTAAC	1 cut(s)	376
LguI	GCTCTTC	1 cut(s)	1281
LmnI	GCTCC	2 cut(s)	258, 1504
Lsp1109I	GCAGC	9 cut(s)	73, 219, 865, 901, 995, 1734, 1774, 1795, 1817
LweI	GCATC	7 cut(s)	170, 250, 304, 472, 556, 694, 1738
MaeI	CTAG	1 cut(s)	696
MaeII	ACGT	2 cut(s)	144, 919
MaeIII	GTNAC	6 cut(s)	367, 936, 1096, 1205, 1526, 1853
MbiI	CCGCTC	1 cut(s)	261
MhlI	GDGCHC	1 cut(s)	225
MluCI	AATT	9 cut(s)	37, 90, 162, 401, 428, 488, 615, 1664, 1678
MlyI	GAGTC	5 cut(s)	92, 754, 857, 1829, 1850
MmeI	TCCRAC	2 cut(s)	840, 1646
MseI	TTAA	2 cut(s)	375, 1146
MslI	CAYNNNNRTG	4 cut(s)	273, 468, 922, 1121
MspA1I	CMGCKG	2 cut(s)	1533, 1808
MspI	CCGG	1 cut(s)	232
MspR9I	CCNGG	3 cut(s)	197, 681, 1701
Mva1269I	GAATGC	1 cut(s)	1597
MvaI	CCWGG	3 cut(s)	197, 681, 1701
MvnI	CGCG	2 cut(s)	141, 246
MwoI	GCNNNNNNNGC	4 cut(s)	270, 566, 884, 1504
NcoI	CCATGG	1 cut(s)	274
NdeI	CATATG	1 cut(s)	1090
NlaIV	GGNNCC	3 cut(s)	318, 603, 1227
NmuCI	GTSAC	3 cut(s)	367, 1096, 1853
NsbI	TGCGCA	1 cut(s)	294
NspI	RCATGY	4 cut(s)	502, 1260, 1722, 1805
OliI	CACNNNNGTG	1 cut(s)	922
PaeI	GCATGC	1 cut(s)	1722
PceI	AGGCCT	1 cut(s)	1408
PciSI	GCTCTTC	1 cut(s)	1281
PctI	GAATGC	1 cut(s)	1597
PfeI	GAWTC	3 cut(s)	49, 663, 1577
PleI	GAGTC	5 cut(s)	92, 754, 857, 1828, 1850
PpsI	GAGTC	5 cut(s)	92, 754, 857, 1828, 1850
PpuMI	RGGWCCY	1 cut(s)	602
Psp124BI	GAGCTC	1 cut(s)	225
Psp5II	RGGWCCY	1 cut(s)	602
Psp6I	CCWGG	3 cut(s)	195, 679, 1699
PspFI	CCCAGC	2 cut(s)	19, 597
PspGI	CCWGG	3 cut(s)	195, 679, 1699
PspN4I	GGNNCC	3 cut(s)	318, 603, 1227
PspPI	GGNCC	8 cut(s)	187, 248, 316, 602, 1168, 1299, 1706, 1758
PspPPI	RGGWCCY	1 cut(s)	602
PstNI	CAGNNNCTG	2 cut(s)	914, 1700
PvuII	CAGCTG	2 cut(s)	1533, 1808
RsaI	GTAC	6 cut(s)	590, 725, 831, 1286, 1341, 1493
RsaNI	GTAC	6 cut(s)	589, 724, 830, 1285, 1340, 1492
RseI	CAYNNNNRTG	4 cut(s)	273, 468, 922, 1121
SacI	GAGCTC	1 cut(s)	225
SalI	GTCGAC	1 cut(s)	1309
SapI	GCTCTTC	1 cut(s)	1281
SaqAI	TTAA	2 cut(s)	375, 1146
Sau96I	GGNCC	8 cut(s)	187, 248, 316, 602, 1168, 1299, 1706, 1758
SchI	GAGTC	5 cut(s)	92, 754, 857, 1829, 1850
ScrFI	CCNGG	3 cut(s)	197, 681, 1701
SduI	GDGCHC	1 cut(s)	225
SfaNI	GCATC	7 cut(s)	170, 250, 304, 472, 556, 694, 1738
SfcI	CTRYAG	2 cut(s)	118, 1645
SinI	GGWCC	7 cut(s)	187, 248, 316, 602, 1168, 1706, 1758
SmiMI	CAYNNNNRTG	4 cut(s)	273, 468, 922, 1121
SphI	GCATGC	1 cut(s)	1722
Sse9I	AATT	9 cut(s)	37, 90, 162, 401, 428, 488, 615, 1664, 1678
SseBI	AGGCCT	1 cut(s)	1408
SspMI	CTAG	1 cut(s)	696
SstI	GAGCTC	1 cut(s)	225
StuI	AGGCCT	1 cut(s)	1408
StyD4I	CCNGG	3 cut(s)	195, 679, 1699
StyI	CCWWGG	2 cut(s)	274, 1792
TaiI	ACGT	2 cut(s)	147, 922
TaqI	TCGA	5 cut(s)	219, 758, 1310, 1425, 1691
TasI	AATT	9 cut(s)	37, 90, 162, 401, 428, 488, 615, 1664, 1678
TatI	WGTACW	2 cut(s)	588, 1491
TauI	GCSGC	6 cut(s)	138, 141, 152, 213, 264, 560
TfiI	GAWTC	3 cut(s)	49, 663, 1577
Tru1I	TTAA	2 cut(s)	375, 1146
Tru9I	TTAA	2 cut(s)	375, 1146
TscAI	CASTG	2 cut(s)	591, 929
TseFI	GTSAC	3 cut(s)	367, 1096, 1853
TseI	GCWGC	9 cut(s)	61, 207, 878, 914, 983, 1722, 1787, 1805, 1808
Tsp45I	GTSAC	3 cut(s)	367, 1096, 1853
TspDTI	ATGAA	9 cut(s)	486, 541, 650, 655, 1206, 1268, 1351, 1473, 1604
TspRI	CASTG	2 cut(s)	591, 929
VpaK11BI	GGWCC	7 cut(s)	187, 248, 316, 602, 1168, 1706, 1758
XagI	CCTNNNNNAGG	1 cut(s)	324
XapI	RAATTY	5 cut(s)	37, 162, 488, 1664, 1678
XceI	RCATGY	4 cut(s)	502, 1260, 1722, 1805
XmiI	GTMKAC	2 cut(s)	1310, 1798
XspI	CTAG	1 cut(s)	696
ZraI	GACGTC	1 cut(s)	145

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.