Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0000473

Physical Location & Seq

Genomic Coordinates Forward (+)

26543480 .. 26550656

Gene Structure 7177 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG256600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1413 bp

ATGCTGGACGGGATGTTCTCCTTTGTCCTTCTTGATACAAGAGACAATACTTTCATGGCGGCTCGAGATGCTATTGGCATAACCCCACTGTACATGGGTTGGGGTCTTGATGGATCAATTTGGTTTGCTTCAGAAATGAAAGCTATAAGTGATGATTGTGAAAGATTCATATCTTTTCCTCCTGGGCATTTATACTCTAGCAAACAAGGAGGGCTGAGAAGGTGGTACAATCCACAATGGTTTTTGGAGAAGATACCATCGACTCCTTATGATCCCATTGTTTTACGTGAGGCTTTTGAGAAGGCTGTGTTAAAGAGACTTATGACAGATGTACCATTCGGTGTTCTTTTGTCTGGAGGATTGGACTCATCACTCGTTGCTGCTGTGGCTTGTTGCTATTTGGCTAAATCAGAAGCTGCATGTCAGTGGGGGTCACAATTACATACCTTTTGTGTTGGCTTGGAGGGTTCTCCAGATTTGAAAGCAGCAAGAGAAGTTGCAGATTATCTTGGAACTCATCATCACGAGTTTCACTTTACTGTTCAGGAAGGCATAGATGCACTTGAAGAAGTTATCTACCATATTGAGACATATGATGTGACAACTGTCAGAGCCAGCACTCCAATGTTTCTTATGTCTCGTAAAATTAAATCTCTGGGAGTAAAAATGGTCCTTTCTGGTGAAGGTTCGGATGAAATCTTTGGTGGCTACTTGTATTTCCACAAGGCACCAAACAAAGAGGAGTTTCACCAAGAAACATGCCAGAAGATTAAAGGTCTTCATCTGTATGACTGTCTGAGGGCCAACAAATCGACATCAGCTTGGGGTGTTGAGGCTCGTGTACCGTTTCTTGACAAAGAATTTATCAAGACTGCTATGGACATTGATCCAGAATGGAAAATGATCAGGCCTGATCTTGGAAGAATTGAGAAGTGGGTCTTACGCAAAGCATTTGATGATGATCAGAAGCCATATTTACCACAGCACATCTTGTACAGGCAGAAGGAGCAGTTTAGTGATGGTGTCGGTTACAGCTGGATTGACGGGTTGAAAGATCATGCAAACAAACAAGTAACAGATTCGATGCTGATGAACGCAAGCTTTGTCTACCCTGAAAACACTCCGACTACAAAAGAAGGATACTACTATAGGACTATCTTTGAGAAATATTTTCCAAAGAATGCTGCGAGATCGACAGTTCCTGGTGGTCCAAGTGTGGCATGCAGCACAGCAAAAGCTGTGGAGTGGGATGCAGAATGGTCCAAGAACCCCGATCCATCTGGTCGTGCTGCACTTGGTATACATGCAGCTGCGTATGAGGAATCCAAAGATGTGAAGACTGGAAGTCTTGTGAGTGACTCTCCGCAGAAACTTGTAAAAGGGGTTGCAGAGAAAGTGGCTGCAGTAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004066 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006082 GO:0006520 GO:0006528 GO:0006529 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009066 GO:0009067 GO:0009314 GO:0009416 GO:0009506 GO:0009628 GO:0009642 GO:0009646 GO:0009651 GO:0009987 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019752 GO:0030054 GO:0032787 GO:0034641 GO:0042538 GO:0042802 GO:0042803 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046983 GO:0050896 GO:0055044 GO:0071704 GO:0072330 GO:0097164 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00250 ko01100 ko01110 map00250 map01100 map01110

Pfam Domains

Protein Families

Asn_synthase GATase_7

Protein Analysis

470

Amino Acids

52.77

Weight (kDa)

5.86

Isoelectric Point (pI)

41.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_7	PF13537	2 - 50	1.3e-13	Glutamine amidotransferase domain
DUF3700	PF12481	2 - 88	6.6e-12	Aluminium induced protein
Asn_synthase	PF00733	95 - 246	1.1e-51	Asparagine synthase
Asn_synthase	PF00733	244 - 396	2.1e-32	Asparagine synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000473)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10240 AT5G10240 AT5G65010 AT5G65010
fragaria_vesca	FvH4_1g20910 FvH4_1g20910 FvH4_1g29202 FvH4_3g23760 FvH4_3g42550
malus_domestica	MD01G1029700.v1.1 MD15G1324200.v1.1
prunus_persica	Prupe.6G186800_v2.0.a1 Prupe.6G186800_v2.0.a1 Prupe.6G186800_v2.0.a1
pyrus_communis	pycom01g05790
rosa_chinensis	RchiOBHm_Chr2g0113551 RchiOBHm_Chr2g0113591 RchiOBHm_Chr2g0113641 RchiOBHm_Chr2g0113651 RchiOBHm_Chr2g0113711 RchiOBHm_Chr2g0113721 RchiOBHm_Chr2g0113831 RchiOBHm_Chr2g0113841 RchiOBHm_Chr2g0113851 RchiOBHm_Chr2g0113911 RchiOBHm_Chr2g0113941 RchiOBHm_Chr2g0113991 RchiOBHm_Chr2g0114001 RchiOBHm_Chr2g0114041 RchiOBHm_Chr2g0114051 RchiOBHm_Chr2g0114061 RchiOBHm_Chr2g0114071 RchiOBHm_Chr2g0114081 RchiOBHm_Chr2g0114101 RchiOBHm_Chr2g0114131 RchiOBHm_Chr2g0114141 RchiOBHm_Chr2g0114261 RchiOBHm_Chr2g0114271 RchiOBHm_Chr2g0114291 RchiOBHm_Chr2g0124041
rosa_laevigata	RLG00000018073 RLG00000018076 RLG00000018078 RLG00000018084 RLG00000018086 RLG00000018088 RLG00000018094 RLG00000018095
rosa_multiflora	Rmu_sc0000881.1_g000007 Rmu_sc0000929.1_g000008 Rmu_sc0000929.1_g000017 Rmu_sc0000929.1_g000029 Rmu_sc0000929.1_g000039 Rmu_sc0001699.1_g000005 Rmu_sc0004283.1_g000004 Rmu_sc0007010.1_g000001 Rmu_sc0007415.1_g000028 Rmu_sc0007415.1_g000045 Rmu_sc0009639.1_g000009 Rmu_sc0010543.1_g000008 Rmu_sc0011941.1_g000022 Rmu_sc0018769.1_g000001
rosa_roxburghii	Rroxscaffold_2G00129150 Rroxscaffold_2G00130050 Rroxscaffold_2G00130070 Rroxscaffold_2G00130090
rosa_rugosa	Rorug02G0188100 Rorug02G0188100 Rorug02G0189700 Rorug02G0190000 Rorug02G0190000 Rorug03G0301100
rosa_samantha	Rh2AG242700 Rh2AG243100 Rh2AG245300 Rh2AG246800 Rh2BG256600 Rh2DG253800 Rh6CG172600
rosa_wichuraiana	Rw0G014630 Rw2G018450 Rw2G018500 Rw2G018980 Rw2G019030 Rw2G019050 Rw2G019100 Rw2G019130 Rw2G019190 Rw2G019420 Rw5G028430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	727
AccI	GTMKAC	2 cut(s)	1107, 1300
AciI	CCGC	2 cut(s)	59, 1364
AclWI	GGATC	4 cut(s)	121, 266, 881, 1268
AcsI	RAATTY	1 cut(s)	860
AcuI	CTGAAG	1 cut(s)	114
AfaI	GTAC	5 cut(s)	92, 227, 333, 843, 995
AfiI	CCNNNNNNNGG	1 cut(s)	917
AgsI	TTSAA	3 cut(s)	481, 566, 1051
AhdI	GACNNNNNGTC	1 cut(s)	1344
AjnI	CCWGG	2 cut(s)	181, 1201
AloI	GAACNNNNNNTCC	1 cut(s)	31
AluBI	AGCT	7 cut(s)	143, 416, 821, 1035, 1101, 1238, 1310
AluI	AGCT	7 cut(s)	143, 416, 821, 1035, 1101, 1238, 1310
Alw26I	GTCTC	4 cut(s)	36, 310, 581, 642
AlwI	GGATC	4 cut(s)	121, 266, 881, 1268
AlwNI	CAGNNNCTG	2 cut(s)	416, 1202
Ama87I	CYCGRG	1 cut(s)	63
AoxI	GGCC	2 cut(s)	801, 908
ApeKI	GCWGC	9 cut(s)	380, 416, 485, 1184, 1224, 1289, 1307, 1310, 1400
ApoI	RAATTY	1 cut(s)	860
AspS9I	GGNCC	4 cut(s)	670, 801, 1208, 1260
AsuHPI	GGTGA	2 cut(s)	692, 740
AvaI	CYCGRG	1 cut(s)	63
AvaII	GGWCC	3 cut(s)	670, 1208, 1260
BaeI	ACNNNNGTAYC	2 cut(s)	315, 348
BanI	GGYRCC	1 cut(s)	727
BauI	CACGAG	2 cut(s)	524, 837
BbsI	GAAGAC	2 cut(s)	770, 1343
BbvI	GCAGC	9 cut(s)	367, 403, 497, 1171, 1236, 1276, 1297, 1319, 1387
BccI	CCATC	4 cut(s)	104, 265, 1013, 1285
BciT130I	CCWGG	2 cut(s)	183, 1203
BciVI	GTATCC	1 cut(s)	1133
BclI	TGATCA	2 cut(s)	903, 961
BcoDI	GTCTC	4 cut(s)	36, 310, 581, 642
BfaI	CTAG	1 cut(s)	198
BfmI	CTRYAG	2 cut(s)	1147, 1401
BfuI	GTATCC	1 cut(s)	1133
Bme1390I	CCNGG	2 cut(s)	183, 1203
Bme18I	GGWCC	3 cut(s)	670, 1208, 1260
BmeRI	GACNNNNNGTC	1 cut(s)	1344
BmeT110I	CYCGRG	1 cut(s)	63
BmgT120I	GGNCC	4 cut(s)	670, 801, 1208, 1260
BmiI	GGNNCC	1 cut(s)	729
BmrFI	CCNGG	2 cut(s)	183, 1203
BmsI	GCATC	4 cut(s)	58, 547, 1074, 1240
BoxI	GACNNNNGTC	1 cut(s)	605
BpiI	GAAGAC	2 cut(s)	770, 1343
BplI	GAGNNNNNCTC	2 cut(s)	1345, 1377
BpmI	CTGGAG	2 cut(s)	375, 456
BsaAI	YACGTR	1 cut(s)	287
BsaBI	GATNNNNATC	2 cut(s)	169, 960
BsaJI	CCNNGG	1 cut(s)	182
Bsc4I	CCNNNNNNNGG	1 cut(s)	917
Bse1I	ACTGG	1 cut(s)	1345
Bse8I	GATNNNNATC	2 cut(s)	169, 960
BseBI	CCWGG	2 cut(s)	183, 1203
BseDI	CCNNGG	1 cut(s)	182
BseGI	GGATG	3 cut(s)	18, 697, 1255
BseJI	GATNNNNATC	2 cut(s)	169, 960
BseLI	CCNNNNNNNGG	1 cut(s)	917
BseMII	CTCAG	2 cut(s)	206, 788
BseNI	ACTGG	1 cut(s)	1345
BseRI	GAGGAG	1 cut(s)	755
BseXI	GCAGC	9 cut(s)	367, 403, 497, 1171, 1236, 1276, 1297, 1319, 1387
BsgI	GTGCAG	1 cut(s)	1275
BshFI	GGCC	2 cut(s)	803, 910
BshNI	GGYRCC	1 cut(s)	727
BsiHKCI	CYCGRG	1 cut(s)	63
BslI	CCNNNNNNNGG	1 cut(s)	917
BsmAI	GTCTC	4 cut(s)	36, 310, 581, 642
BsmI	GAATGC	1 cut(s)	1186
BsnI	GGCC	2 cut(s)	803, 910
BsoBI	CYCGRG	1 cut(s)	63
Bsp1407I	TGTACA	2 cut(s)	90, 993
Bsp143I	GATC	9 cut(s)	113, 271, 886, 903, 913, 961, 1054, 1190, 1273
BspACI	CCGC	2 cut(s)	59, 1364
BspANI	GGCC	2 cut(s)	803, 910
BspCNI	CTCAG	2 cut(s)	207, 789
BspLI	GGNNCC	1 cut(s)	729
BspMAI	CTGCAG	1 cut(s)	1405
BspPI	GGATC	4 cut(s)	121, 266, 881, 1268
BspT107I	GGYRCC	1 cut(s)	727
BsrGI	TGTACA	2 cut(s)	90, 993
BsrI	ACTGG	1 cut(s)	1345
BssECI	CCNNGG	1 cut(s)	182
BssMI	GATC	9 cut(s)	113, 271, 886, 903, 913, 961, 1054, 1190, 1273
BssNAI	GTATAC	1 cut(s)	1301
BssSI	CACGAG	2 cut(s)	524, 837
Bst1107I	GTATAC	1 cut(s)	1301
Bst2BI	CACGAG	2 cut(s)	524, 837
Bst2UI	CCWGG	2 cut(s)	183, 1203
Bst4CI	ACNGT	6 cut(s)	90, 541, 607, 794, 846, 1198
BstAUI	TGTACA	2 cut(s)	90, 993
BstBAI	YACGTR	1 cut(s)	287
BstC8I	GCNNGC	3 cut(s)	616, 1099, 1222
BstDEI	CTNAG	2 cut(s)	215, 797
BstF5I	GGATG	3 cut(s)	18, 697, 1255
BstKTI	GATC	9 cut(s)	116, 274, 889, 906, 916, 964, 1057, 1193, 1276
BstMAI	GTCTC	4 cut(s)	36, 310, 581, 642
BstMBI	GATC	9 cut(s)	113, 271, 886, 903, 913, 961, 1054, 1190, 1273
BstMWI	GCNNNNNNNGC	3 cut(s)	68, 386, 1006
BstNI	CCWGG	2 cut(s)	183, 1203
BstNSI	RCATGY	4 cut(s)	423, 762, 1224, 1307
BstPAI	GACNNNNGTC	1 cut(s)	605
BstSCI	CCNGG	2 cut(s)	181, 1201
BstSFI	CTRYAG	2 cut(s)	1147, 1401
BstV1I	GCAGC	9 cut(s)	367, 403, 497, 1171, 1236, 1276, 1297, 1319, 1387
BstV2I	GAAGAC	2 cut(s)	770, 1343
BstZ17I	GTATAC	1 cut(s)	1301
BsuI	GTATCC	1 cut(s)	1133
BsuRI	GGCC	2 cut(s)	803, 910
BtsCI	GGATG	3 cut(s)	18, 697, 1255
BtsIMutI	CAGTG	2 cut(s)	86, 431
Cac8I	GCNNGC	3 cut(s)	616, 1099, 1222
CaiI	CAGNNNCTG	2 cut(s)	416, 1202
Cfr13I	GGNCC	4 cut(s)	670, 801, 1208, 1260
Csp6I	GTAC	5 cut(s)	91, 226, 332, 842, 994
CspCI	CAANNNNNGTGG	2 cut(s)	1221, 1256
CviAII	CATG	7 cut(s)	55, 94, 420, 759, 1058, 1221, 1304
CviQI	GTAC	5 cut(s)	91, 226, 332, 842, 994
DdeI	CTNAG	2 cut(s)	215, 797
DpnI	GATC	9 cut(s)	115, 273, 888, 905, 915, 963, 1056, 1192, 1275
DpnII	GATC	9 cut(s)	113, 271, 886, 903, 913, 961, 1054, 1190, 1273
DriI	GACNNNNNGTC	1 cut(s)	1344
Eam1105I	GACNNNNNGTC	1 cut(s)	1344
Eco147I	AGGCCT	1 cut(s)	910
Eco47I	GGWCC	3 cut(s)	670, 1208, 1260
Eco57I	CTGAAG	1 cut(s)	114
Eco88I	CYCGRG	1 cut(s)	63
EcoRII	CCWGG	2 cut(s)	181, 1201
FaeI	CATG	7 cut(s)	58, 97, 423, 762, 1061, 1224, 1307
FalI	AAGNNNNNCTT	2 cut(s)	923, 955
FatI	CATG	7 cut(s)	54, 93, 419, 758, 1057, 1220, 1303
FauNDI	CATATG	1 cut(s)	592
FbaI	TGATCA	2 cut(s)	903, 961
FblI	GTMKAC	2 cut(s)	1107, 1300
FokI	GGATG	3 cut(s)	25, 704, 1262
FspBI	CTAG	1 cut(s)	198
GsuI	CTGGAG	2 cut(s)	375, 456
HaeIII	GGCC	2 cut(s)	803, 910
Hin1II	CATG	7 cut(s)	58, 97, 423, 762, 1061, 1224, 1307
HindIII	AAGCTT	1 cut(s)	1099
HinfI	GANTC	6 cut(s)	165, 262, 365, 1079, 1322, 1358
HphI	GGTGA	2 cut(s)	692, 740
Hpy166II	GTNNAC	3 cut(s)	842, 1108, 1301
Hpy188I	TCNGA	7 cut(s)	133, 412, 611, 691, 798, 966, 1125
Hpy8I	GTNNAC	3 cut(s)	842, 1108, 1301
HpyAV	CCTTC	7 cut(s)	38, 213, 295, 542, 677, 997, 1130
HpyCH4III	ACNGT	6 cut(s)	90, 541, 607, 794, 846, 1198
HpyCH4IV	ACGT	1 cut(s)	286
HpyF10VI	GCNNNNNNNGC	3 cut(s)	68, 386, 1006
HpyF3I	CTNAG	2 cut(s)	215, 797
HpySE526I	ACGT	1 cut(s)	286
Hsp92II	CATG	7 cut(s)	58, 97, 423, 762, 1061, 1224, 1307
Ksp22I	TGATCA	2 cut(s)	903, 961
Kzo9I	GATC	9 cut(s)	113, 271, 886, 903, 913, 961, 1054, 1190, 1273
LmnI	GCTCC	1 cut(s)	1006
Lsp1109I	GCAGC	9 cut(s)	367, 403, 497, 1171, 1236, 1276, 1297, 1319, 1387
LweI	GCATC	4 cut(s)	58, 547, 1074, 1240
MaeI	CTAG	1 cut(s)	198
MaeII	ACGT	1 cut(s)	286
MaeIII	GTNAC	5 cut(s)	432, 598, 1028, 1072, 1355
MalI	GATC	9 cut(s)	115, 273, 888, 905, 915, 963, 1056, 1192, 1275
MboI	GATC	9 cut(s)	113, 271, 886, 903, 913, 961, 1054, 1190, 1273
MboII	GAAGA	6 cut(s)	262, 578, 770, 778, 933, 1348
MluCI	AATT	5 cut(s)	117, 437, 645, 860, 924
MlyI	GAGTC	3 cut(s)	256, 359, 1352
MmeI	TCCRAC	1 cut(s)	1148
MnlI	CCTC	9 cut(s)	189, 203, 283, 350, 457, 733, 792, 826, 1312
MseI	TTAA	3 cut(s)	311, 648, 771
MslI	CAYNNNNRTG	3 cut(s)	424, 623, 786
MspA1I	CMGCKG	2 cut(s)	1035, 1310
MspR9I	CCNGG	2 cut(s)	183, 1203
Mva1269I	GAATGC	1 cut(s)	1186
MvaI	CCWGG	2 cut(s)	183, 1203
MwoI	GCNNNNNNNGC	3 cut(s)	68, 386, 1006
NdeI	CATATG	1 cut(s)	592
NdeII	GATC	9 cut(s)	113, 271, 886, 903, 913, 961, 1054, 1190, 1273
NlaIII	CATG	7 cut(s)	58, 97, 423, 762, 1061, 1224, 1307
NlaIV	GGNNCC	1 cut(s)	729
NmuCI	GTSAC	3 cut(s)	432, 598, 1355
NspI	RCATGY	4 cut(s)	423, 762, 1224, 1307
PaeI	GCATGC	1 cut(s)	1224
PaeR7I	CTCGAG	1 cut(s)	63
PceI	AGGCCT	1 cut(s)	910
PctI	GAATGC	1 cut(s)	1186
PfeI	GAWTC	3 cut(s)	165, 1079, 1322
PleI	GAGTC	3 cut(s)	256, 359, 1352
PpsI	GAGTC	3 cut(s)	256, 359, 1352
Ppu21I	YACGTR	1 cut(s)	287
PshAI	GACNNNNGTC	1 cut(s)	605
Psp6I	CCWGG	2 cut(s)	181, 1201
PspGI	CCWGG	2 cut(s)	181, 1201
PspN4I	GGNNCC	1 cut(s)	729
PspPI	GGNCC	4 cut(s)	670, 801, 1208, 1260
PstI	CTGCAG	1 cut(s)	1405
PstNI	CAGNNNCTG	2 cut(s)	416, 1202
PvuII	CAGCTG	2 cut(s)	1035, 1310
RsaI	GTAC	5 cut(s)	92, 227, 333, 843, 995
RsaNI	GTAC	5 cut(s)	91, 226, 332, 842, 994
RseI	CAYNNNNRTG	3 cut(s)	424, 623, 786
SaqAI	TTAA	3 cut(s)	311, 648, 771
Sau3AI	GATC	9 cut(s)	113, 271, 886, 903, 913, 961, 1054, 1190, 1273
Sau96I	GGNCC	4 cut(s)	670, 801, 1208, 1260
SchI	GAGTC	3 cut(s)	256, 359, 1352
ScrFI	CCNGG	2 cut(s)	183, 1203
SfaNI	GCATC	4 cut(s)	58, 547, 1074, 1240
SfcI	CTRYAG	2 cut(s)	1147, 1401
Sfr274I	CTCGAG	1 cut(s)	63
SinI	GGWCC	3 cut(s)	670, 1208, 1260
SlaI	CTCGAG	1 cut(s)	63
SmiMI	CAYNNNNRTG	3 cut(s)	424, 623, 786
SmlI	CTYRAG	1 cut(s)	63
SmoI	CTYRAG	1 cut(s)	63
SphI	GCATGC	1 cut(s)	1224
Sse9I	AATT	5 cut(s)	117, 437, 645, 860, 924
SseBI	AGGCCT	1 cut(s)	910
SsiI	CCGC	2 cut(s)	59, 1364
SspI	AATATT	1 cut(s)	1169
SspMI	CTAG	1 cut(s)	198
StuI	AGGCCT	1 cut(s)	910
StyD4I	CCNGG	2 cut(s)	181, 1201
TaaI	ACNGT	6 cut(s)	90, 541, 607, 794, 846, 1198
TaiI	ACGT	1 cut(s)	289
TaqI	TCGA	5 cut(s)	64, 260, 812, 1082, 1193
TasI	AATT	5 cut(s)	117, 437, 645, 860, 924
TatI	WGTACW	2 cut(s)	90, 993
TauI	GCSGC	1 cut(s)	62
TfiI	GAWTC	3 cut(s)	165, 1079, 1322
Tru1I	TTAA	3 cut(s)	311, 648, 771
Tru9I	TTAA	3 cut(s)	311, 648, 771
TscAI	CASTG	2 cut(s)	93, 431
TseFI	GTSAC	3 cut(s)	432, 598, 1355
TseI	GCWGC	9 cut(s)	380, 416, 485, 1184, 1224, 1289, 1307, 1310, 1400
Tsp45I	GTSAC	3 cut(s)	432, 598, 1355
TspDTI	ATGAA	6 cut(s)	43, 152, 157, 708, 770, 1106
TspRI	CASTG	2 cut(s)	93, 431
VpaK11BI	GGWCC	3 cut(s)	670, 1208, 1260
XapI	RAATTY	1 cut(s)	860
XceI	RCATGY	4 cut(s)	423, 762, 1224, 1307
XhoI	CTCGAG	1 cut(s)	63
XmiI	GTMKAC	2 cut(s)	1107, 1300
XspI	CTAG	1 cut(s)	198

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.