Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0000473

Physical Location & Seq

Genomic Coordinates Forward (+)

25871775 .. 25877446

Gene Structure 5672 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ48704

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1245 bp

ATGAAAGCTGTAAGTGATGATTGTGAAAGGTTCACATCTTTTCCTCCTGGGCATTTATACTCTAGCAAACAAGGAGGGCTGAGAAGGTGGTACAATCCACAATGGTTTTTGGAGAAGATACCATCGACTCCTTGTGATCCAATTGTTTTACGCGAGGCTTTTGAGAAGGCTGTGTTAATGAGACTTATGACAGATGTACCATTCGGTGTTCTTTTGTCTGGAGGATTGAACTCATCACTCGTAGCTGCTGTGGCTTGTCGCTATTTGGCTAAATCAGAAGCTGCACGTCAGTGGGGGTCACAATTACATACCTTTTGTGTTGGCTTGGAGGGTTCTCCAGATTTGAAAGCAGCAAGAGAAGTTGCAGATTATCTTGGAACTCATCATCACGAGTTGCACTTTACTGTTCGGGTAATTTATAAGGGATGTAAGAAGAATACTTTTACTAAGGCTCAAGATTTTCAGGAAGGCATAGATGCACTTGAAGAAGTTATCTACCATATTGAGACATATGTGACCACTGTCAGAGCCAGCACTCCAATGTTTCTTATGTCTCGTAAAATTAAATCTCTAGGAGTAAAAATGGTCCTCTTTGGTGAAGGTTCGGATGAAATCTTTGGTGGCTACTTGTATTTCCACAAGGCACCAAACAAGGAGAAGTTTCACCAAGAAACATGCCAGAAGATTAAAGGTCTTCATCTGTATGACTGTCTGAGGGCCAACAAATCGGCATCAGCTGGGGGTGTTGAGGCTCGTGTACCGTTTCTTGACAAAGAATTTGTCAAGACTGCTATGGACATTGATCCAGAATGGAAAATGATCAGGCCTGATCTTGGAAGAATTGAGAAGTGGGTCTTACGCAAAGCATTCGATGATGATCAGAAGCCATATTTACCAAAGCACATCTTGTACAGGCAGAAGGAGCAGTTTAGTGATGGTGTCGGTTACAGCTGGATTGAGGGGTTGAAAGATCATGCAAACAAACAAGTAACAGATTCGATGCTGATGAACGCAAGCTTTGTCTACCCTGAAAACACTCCGACTACAAAAGAAGGATACTACTATAGGACTATCTTTGAGAAATATTTTCCAAAGAATGCTGCGAGATCGACAGTTCCTGGTGGACCAAGTGTGGCATGCAGCACAGCAAAAGCTGTGGAGTGGGATGCAGAATGTGTCAGCGTGAGTTATGAACATGCCAGGAATCACAGAACATGGAAGATCATCATGGCATGCCAACATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004066 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006082 GO:0006520 GO:0006528 GO:0006529 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009066 GO:0009067 GO:0009314 GO:0009416 GO:0009506 GO:0009628 GO:0009642 GO:0009646 GO:0009651 GO:0009987 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019752 GO:0030054 GO:0032787 GO:0034641 GO:0042538 GO:0042802 GO:0042803 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046983 GO:0050896 GO:0055044 GO:0071704 GO:0072330 GO:0097164 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00250 ko01100 ko01110 map00250 map01100 map01110

Pfam Domains

Protein Families

Asn_synthase GATase_7

Protein Analysis

414

Amino Acids

47.16

Weight (kDa)

8.62

Isoelectric Point (pI)

41.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asn_synthase	PF00733	50 - 218	6e-43	Asparagine synthase
Asn_synthase	PF00733	216 - 368	8.6e-32	Asparagine synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000473)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10240 AT5G10240 AT5G65010 AT5G65010
fragaria_vesca	FvH4_1g20910 FvH4_1g20910 FvH4_1g29202 FvH4_3g23760 FvH4_3g42550
malus_domestica	MD01G1029700.v1.1 MD15G1324200.v1.1
prunus_persica	Prupe.6G186800_v2.0.a1 Prupe.6G186800_v2.0.a1 Prupe.6G186800_v2.0.a1
pyrus_communis	pycom01g05790
rosa_chinensis	RchiOBHm_Chr2g0113551 RchiOBHm_Chr2g0113591 RchiOBHm_Chr2g0113641 RchiOBHm_Chr2g0113651 RchiOBHm_Chr2g0113711 RchiOBHm_Chr2g0113721 RchiOBHm_Chr2g0113831 RchiOBHm_Chr2g0113841 RchiOBHm_Chr2g0113851 RchiOBHm_Chr2g0113911 RchiOBHm_Chr2g0113941 RchiOBHm_Chr2g0113991 RchiOBHm_Chr2g0114001 RchiOBHm_Chr2g0114041 RchiOBHm_Chr2g0114051 RchiOBHm_Chr2g0114061 RchiOBHm_Chr2g0114071 RchiOBHm_Chr2g0114081 RchiOBHm_Chr2g0114101 RchiOBHm_Chr2g0114131 RchiOBHm_Chr2g0114141 RchiOBHm_Chr2g0114261 RchiOBHm_Chr2g0114271 RchiOBHm_Chr2g0114291 RchiOBHm_Chr2g0124041
rosa_laevigata	RLG00000018073 RLG00000018076 RLG00000018078 RLG00000018084 RLG00000018086 RLG00000018088 RLG00000018094 RLG00000018095
rosa_multiflora	Rmu_sc0000881.1_g000007 Rmu_sc0000929.1_g000008 Rmu_sc0000929.1_g000017 Rmu_sc0000929.1_g000029 Rmu_sc0000929.1_g000039 Rmu_sc0001699.1_g000005 Rmu_sc0004283.1_g000004 Rmu_sc0007010.1_g000001 Rmu_sc0007415.1_g000028 Rmu_sc0007415.1_g000045 Rmu_sc0009639.1_g000009 Rmu_sc0010543.1_g000008 Rmu_sc0011941.1_g000022 Rmu_sc0018769.1_g000001
rosa_roxburghii	Rroxscaffold_2G00129150 Rroxscaffold_2G00130050 Rroxscaffold_2G00130070 Rroxscaffold_2G00130090
rosa_rugosa	Rorug02G0188100 Rorug02G0188100 Rorug02G0189700 Rorug02G0190000 Rorug02G0190000 Rorug03G0301100
rosa_samantha	Rh2AG242700 Rh2AG243100 Rh2AG245300 Rh2AG246800 Rh2BG256600 Rh2DG253800 Rh6CG172600
rosa_wichuraiana	Rw0G014630 Rw2G018450 Rw2G018500 Rw2G018980 Rw2G019030 Rw2G019050 Rw2G019100 Rw2G019130 Rw2G019190 Rw2G019420 Rw5G028430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	420
AccB1I	GGYRCC	1 cut(s)	643
AccI	GTMKAC	1 cut(s)	1023
AccII	CGCG	1 cut(s)	153
AclWI	GGATC	2 cut(s)	131, 797
AcsI	RAATTY	1 cut(s)	776
AfaI	GTAC	4 cut(s)	92, 198, 759, 911
AfiI	CCNNNNNNNGG	1 cut(s)	833
AgsI	TTSAA	4 cut(s)	229, 346, 485, 967
AjiI	CACGTC	1 cut(s)	287
AjnI	CCWGG	3 cut(s)	46, 1117, 1199
AleI	CACNNNNGTG	1 cut(s)	289
AluBI	AGCT	7 cut(s)	8, 245, 281, 737, 951, 1017, 1154
AluI	AGCT	7 cut(s)	8, 245, 281, 737, 951, 1017, 1154
Alw26I	GTCTC	3 cut(s)	175, 500, 558
AlwI	GGATC	2 cut(s)	131, 797
AlwNI	CAGNNNCTG	2 cut(s)	281, 1118
AoxI	GGCC	2 cut(s)	717, 824
ApeKI	GCWGC	5 cut(s)	245, 281, 350, 1100, 1140
ApoI	RAATTY	1 cut(s)	776
ArsI	GACNNNNNNTTYG	4 cut(s)	761, 765, 793, 797
AspS9I	GGNCC	3 cut(s)	586, 717, 1124
AsuHPI	GGTGA	2 cut(s)	608, 656
AvaII	GGWCC	2 cut(s)	586, 1124
BaeI	ACNNNNGTAYC	2 cut(s)	180, 213
BanI	GGYRCC	1 cut(s)	643
BauI	CACGAG	2 cut(s)	389, 753
BbsI	GAAGAC	1 cut(s)	686
BbvI	GCAGC	5 cut(s)	232, 268, 362, 1087, 1152
BccI	CCATC	2 cut(s)	130, 929
BcgI	CGANNNNNNTGC	2 cut(s)	850, 884
BciT130I	CCWGG	3 cut(s)	48, 1119, 1201
BciVI	GTATCC	1 cut(s)	1049
BclI	TGATCA	2 cut(s)	819, 877
BcoDI	GTCTC	3 cut(s)	175, 500, 558
BfaI	CTAG	2 cut(s)	63, 572
BfmI	CTRYAG	1 cut(s)	1063
BfuI	GTATCC	1 cut(s)	1049
BisI	GCNGC	5 cut(s)	246, 282, 351, 1101, 1141
BlsI	GCNGC	5 cut(s)	247, 283, 352, 1102, 1142
Bme1390I	CCNGG	3 cut(s)	48, 1119, 1201
Bme18I	GGWCC	2 cut(s)	586, 1124
BmgBI	CACGTC	1 cut(s)	287
BmgT120I	GGNCC	3 cut(s)	586, 717, 1124
BmiI	GGNNCC	1 cut(s)	645
BmrFI	CCNGG	3 cut(s)	48, 1119, 1201
BmsI	GCATC	4 cut(s)	466, 740, 990, 1156
BoxI	GACNNNNGTC	1 cut(s)	521
BpiI	GAAGAC	1 cut(s)	686
BpmI	CTGGAG	2 cut(s)	240, 321
BpuEI	CTTGAG	1 cut(s)	438
BsaBI	GATNNNNATC	1 cut(s)	876
BsaJI	CCNNGG	1 cut(s)	47
Bsc4I	CCNNNNNNNGG	1 cut(s)	833
Bse8I	GATNNNNATC	1 cut(s)	876
BseBI	CCWGG	3 cut(s)	48, 1119, 1201
BseDI	CCNNGG	1 cut(s)	47
BseGI	GGATG	3 cut(s)	431, 613, 1171
BseJI	GATNNNNATC	1 cut(s)	876
BseLI	CCNNNNNNNGG	1 cut(s)	833
BseMII	CTCAG	2 cut(s)	71, 704
BseXI	GCAGC	5 cut(s)	232, 268, 362, 1087, 1152
BseYI	CCCAGC	1 cut(s)	737
BsgI	GTGCAG	1 cut(s)	267
Bsh1236I	CGCG	1 cut(s)	153
BshFI	GGCC	2 cut(s)	719, 826
BshNI	GGYRCC	1 cut(s)	643
BslI	CCNNNNNNNGG	1 cut(s)	833
BsmAI	GTCTC	3 cut(s)	175, 500, 558
BsmI	GAATGC	2 cut(s)	866, 1102
BsnI	GGCC	2 cut(s)	719, 826
Bsp1407I	TGTACA	1 cut(s)	909
Bsp143I	GATC	8 cut(s)	136, 802, 819, 829, 877, 970, 1106, 1221
BspANI	GGCC	2 cut(s)	719, 826
BspCNI	CTCAG	2 cut(s)	72, 705
BspFNI	CGCG	1 cut(s)	153
BspLI	GGNNCC	1 cut(s)	645
BspPI	GGATC	2 cut(s)	131, 797
BspT107I	GGYRCC	1 cut(s)	643
BsrGI	TGTACA	1 cut(s)	909
BssECI	CCNNGG	1 cut(s)	47
BssMI	GATC	8 cut(s)	136, 802, 819, 829, 877, 970, 1106, 1221
BssSI	CACGAG	2 cut(s)	389, 753
Bst2BI	CACGAG	2 cut(s)	389, 753
Bst2UI	CCWGG	3 cut(s)	48, 1119, 1201
Bst4CI	ACNGT	5 cut(s)	406, 523, 710, 762, 1114
BstAUI	TGTACA	1 cut(s)	909
BstC8I	GCNNGC	4 cut(s)	532, 1015, 1138, 1234
BstDEI	CTNAG	3 cut(s)	80, 447, 713
BstF5I	GGATG	3 cut(s)	431, 613, 1171
BstFNI	CGCG	1 cut(s)	153
BstKTI	GATC	8 cut(s)	139, 805, 822, 832, 880, 973, 1109, 1224
BstMAI	GTCTC	3 cut(s)	175, 500, 558
BstMBI	GATC	8 cut(s)	136, 802, 819, 829, 877, 970, 1106, 1221
BstMWI	GCNNNNNNNGC	2 cut(s)	251, 922
BstNI	CCWGG	3 cut(s)	48, 1119, 1201
BstNSI	RCATGY	4 cut(s)	678, 1140, 1199, 1236
BstPAI	GACNNNNGTC	1 cut(s)	521
BstSCI	CCNGG	3 cut(s)	46, 1117, 1199
BstSFI	CTRYAG	1 cut(s)	1063
BstUI	CGCG	1 cut(s)	153
BstV1I	GCAGC	5 cut(s)	232, 268, 362, 1087, 1152
BstV2I	GAAGAC	1 cut(s)	686
BsuI	GTATCC	1 cut(s)	1049
BsuRI	GGCC	2 cut(s)	719, 826
BtrI	CACGTC	1 cut(s)	287
BtsCI	GGATG	3 cut(s)	431, 613, 1171
BtsIMutI	CAGTG	2 cut(s)	296, 519
Cac8I	GCNNGC	4 cut(s)	532, 1015, 1138, 1234
CaiI	CAGNNNCTG	2 cut(s)	281, 1118
Cfr13I	GGNCC	3 cut(s)	586, 717, 1124
Csp6I	GTAC	4 cut(s)	91, 197, 758, 910
CspCI	CAANNNNNGTGG	2 cut(s)	1137, 1172
CviAII	CATG	7 cut(s)	675, 974, 1137, 1196, 1215, 1228, 1233
CviQI	GTAC	4 cut(s)	91, 197, 758, 910
DdeI	CTNAG	3 cut(s)	80, 447, 713
DpnI	GATC	8 cut(s)	138, 804, 821, 831, 879, 972, 1108, 1223
DpnII	GATC	8 cut(s)	136, 802, 819, 829, 877, 970, 1106, 1221
Eco147I	AGGCCT	1 cut(s)	826
Eco47I	GGWCC	2 cut(s)	586, 1124
EcoRII	CCWGG	3 cut(s)	46, 1117, 1199
FaeI	CATG	7 cut(s)	678, 977, 1140, 1199, 1218, 1231, 1236
FalI	AAGNNNNNCTT	4 cut(s)	839, 871, 890, 922
FatI	CATG	7 cut(s)	674, 973, 1136, 1195, 1214, 1227, 1232
FauNDI	CATATG	1 cut(s)	511
FbaI	TGATCA	2 cut(s)	819, 877
FblI	GTMKAC	1 cut(s)	1023
Fnu4HI	GCNGC	5 cut(s)	246, 282, 351, 1101, 1141
FokI	GGATG	3 cut(s)	438, 620, 1178
Fsp4HI	GCNGC	5 cut(s)	246, 282, 351, 1101, 1141
FspBI	CTAG	2 cut(s)	63, 572
GluI	GCNGC	5 cut(s)	246, 282, 351, 1101, 1141
GsaI	CCCAGC	1 cut(s)	741
GsuI	CTGGAG	2 cut(s)	240, 321
HaeIII	GGCC	2 cut(s)	719, 826
Hin1II	CATG	7 cut(s)	678, 977, 1140, 1199, 1218, 1231, 1236
HindIII	AAGCTT	1 cut(s)	1015
HinfI	GANTC	3 cut(s)	127, 995, 1204
HphI	GGTGA	2 cut(s)	608, 656
Hpy166II	GTNNAC	4 cut(s)	33, 758, 1024, 1124
Hpy188I	TCNGA	6 cut(s)	277, 527, 607, 714, 882, 1041
Hpy188III	TCNNGA	8 cut(s)	219, 338, 389, 455, 464, 767, 784, 806
Hpy8I	GTNNAC	4 cut(s)	33, 758, 1024, 1124
HpyAV	CCTTC	6 cut(s)	78, 160, 461, 593, 913, 1046
HpyCH4III	ACNGT	5 cut(s)	406, 523, 710, 762, 1114
HpyCH4IV	ACGT	1 cut(s)	286
HpyCH4V	TGCA	7 cut(s)	284, 365, 397, 479, 977, 1140, 1169
HpyF10VI	GCNNNNNNNGC	2 cut(s)	251, 922
HpyF3I	CTNAG	3 cut(s)	80, 447, 713
HpySE526I	ACGT	1 cut(s)	286
Hsp92II	CATG	7 cut(s)	678, 977, 1140, 1199, 1218, 1231, 1236
Ksp22I	TGATCA	2 cut(s)	819, 877
Kzo9I	GATC	8 cut(s)	136, 802, 819, 829, 877, 970, 1106, 1221
LmnI	GCTCC	1 cut(s)	922
Lsp1109I	GCAGC	5 cut(s)	232, 268, 362, 1087, 1152
LweI	GCATC	4 cut(s)	466, 740, 990, 1156
MaeI	CTAG	2 cut(s)	63, 572
MaeII	ACGT	1 cut(s)	286
MaeIII	GTNAC	4 cut(s)	297, 514, 944, 988
MalI	GATC	8 cut(s)	138, 804, 821, 831, 879, 972, 1108, 1223
MboI	GATC	8 cut(s)	136, 802, 819, 829, 877, 970, 1106, 1221
MboII	GAAGA	7 cut(s)	127, 445, 497, 686, 694, 849, 1231
MfeI	CAATTG	1 cut(s)	141
MluCI	AATT	6 cut(s)	141, 302, 414, 561, 776, 840
MlyI	GAGTC	1 cut(s)	121
MmeI	TCCRAC	1 cut(s)	1064
MnlI	CCTC	9 cut(s)	54, 68, 148, 215, 322, 599, 708, 742, 952
MseI	TTAA	3 cut(s)	176, 564, 687
MslI	CAYNNNNRTG	3 cut(s)	289, 539, 702
MspA1I	CMGCKG	2 cut(s)	737, 951
MspR9I	CCNGG	3 cut(s)	48, 1119, 1201
MunI	CAATTG	1 cut(s)	141
Mva1269I	GAATGC	2 cut(s)	866, 1102
MvaI	CCWGG	3 cut(s)	48, 1119, 1201
MvnI	CGCG	1 cut(s)	153
MwoI	GCNNNNNNNGC	2 cut(s)	251, 922
NdeI	CATATG	1 cut(s)	511
NdeII	GATC	8 cut(s)	136, 802, 819, 829, 877, 970, 1106, 1221
NlaIII	CATG	7 cut(s)	678, 977, 1140, 1199, 1218, 1231, 1236
NlaIV	GGNNCC	1 cut(s)	645
NmuCI	GTSAC	2 cut(s)	297, 514
NspI	RCATGY	4 cut(s)	678, 1140, 1199, 1236
OliI	CACNNNNGTG	1 cut(s)	289
PaeI	GCATGC	2 cut(s)	1140, 1236
PceI	AGGCCT	1 cut(s)	826
PctI	GAATGC	2 cut(s)	866, 1102
PfeI	GAWTC	2 cut(s)	995, 1204
PkrI	GCNGC	5 cut(s)	247, 283, 352, 1102, 1142
PleI	GAGTC	1 cut(s)	121
PpsI	GAGTC	1 cut(s)	121
PshAI	GACNNNNGTC	1 cut(s)	521
PsiI	TTATAA	1 cut(s)	420
Psp6I	CCWGG	3 cut(s)	46, 1117, 1199
PspFI	CCCAGC	1 cut(s)	737
PspGI	CCWGG	3 cut(s)	46, 1117, 1199
PspN4I	GGNNCC	1 cut(s)	645
PspPI	GGNCC	3 cut(s)	586, 717, 1124
PstNI	CAGNNNCTG	2 cut(s)	281, 1118
PvuII	CAGCTG	2 cut(s)	737, 951
RsaI	GTAC	4 cut(s)	92, 198, 759, 911
RsaNI	GTAC	4 cut(s)	91, 197, 758, 910
RseI	CAYNNNNRTG	3 cut(s)	289, 539, 702
SaqAI	TTAA	3 cut(s)	176, 564, 687
SatI	GCNGC	5 cut(s)	246, 282, 351, 1101, 1141
Sau3AI	GATC	8 cut(s)	136, 802, 819, 829, 877, 970, 1106, 1221
Sau96I	GGNCC	3 cut(s)	586, 717, 1124
SchI	GAGTC	1 cut(s)	121
ScrFI	CCNGG	3 cut(s)	48, 1119, 1201
SfaNI	GCATC	4 cut(s)	466, 740, 990, 1156
SfcI	CTRYAG	1 cut(s)	1063
SinI	GGWCC	2 cut(s)	586, 1124
SmiMI	CAYNNNNRTG	3 cut(s)	289, 539, 702
SmlI	CTYRAG	1 cut(s)	453
SmoI	CTYRAG	1 cut(s)	453
SphI	GCATGC	2 cut(s)	1140, 1236
Sse9I	AATT	6 cut(s)	141, 302, 414, 561, 776, 840
SseBI	AGGCCT	1 cut(s)	826
SspI	AATATT	1 cut(s)	1085
SspMI	CTAG	2 cut(s)	63, 572
StuI	AGGCCT	1 cut(s)	826
StyD4I	CCNGG	3 cut(s)	46, 1117, 1199
TaaI	ACNGT	5 cut(s)	406, 523, 710, 762, 1114
TaiI	ACGT	1 cut(s)	289
TaqI	TCGA	4 cut(s)	125, 870, 998, 1109
TasI	AATT	6 cut(s)	141, 302, 414, 561, 776, 840
TatI	WGTACW	1 cut(s)	909
TfiI	GAWTC	2 cut(s)	995, 1204
Tru1I	TTAA	3 cut(s)	176, 564, 687
Tru9I	TTAA	3 cut(s)	176, 564, 687
TscAI	CASTG	2 cut(s)	296, 526
TseFI	GTSAC	2 cut(s)	297, 514
TseI	GCWGC	5 cut(s)	245, 281, 350, 1100, 1140
Tsp45I	GTSAC	2 cut(s)	297, 514
TspDTI	ATGAA	5 cut(s)	17, 624, 686, 1022, 1206
TspRI	CASTG	2 cut(s)	296, 526
VpaK11BI	GGWCC	2 cut(s)	586, 1124
XapI	RAATTY	1 cut(s)	776
XceI	RCATGY	4 cut(s)	678, 1140, 1199, 1236
XmiI	GTMKAC	1 cut(s)	1023
XspI	CTAG	2 cut(s)	63, 572

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.