Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0000473

Physical Location & Seq

Genomic Coordinates Reverse (-)

64990421 .. 65000061

Gene Structure 9641 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00129150.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1740 bp

ATGTCGGTCGCCCCCGATCCCAAATCCGAGAATGCTCCCCTCCGCTGCACAAGCAAGCTTCTCACACCAAATACGAACGGTACCGGAAGACCCTTCGACGCAAGAAACGATCCAATTGCCGCCGCCTCGCTACCAAAAACCCCGGGGTTTTGGTACACTCGGCTCCGAAAAAGATGGTTGCGGCATCGCGGTCCTGATTGGAGCGGGTTGCATTGCCATGGAGATTGTTACCTTGCTCATCAGCGATTGGCTATTGTGGACCCTGCCTCAGGGGATCAGCCGCTCTACAACGAGCACAAGACCGTCATTGTCACGGTTAATGGTGAGATATATAACCACAAGCAGTTGAGAGAAACATTGAAGGGTCATCGATTCCGAACTGGCAGTGACTGTGAAGTGATAGCGCACCTTTACGAGGAACATGGGGAAGAATTTGTTGACATGCTGGACGGGATGTTCTCCTTTGTCCTCCTTGATACAAGAGACAACACTTTCATGGCTGCTCGAGATGCTATTGGCATTACCCCACTTTACATGGGTTGGGGTCTTGATGGATCAATTTGGTTTGCTTCAGAAATGAAAGCTATAAGTGATGATTGTGAAAGATTCATATCTTTTCCTCCCGGGCATCTATACTCTAGCAAACAAGGAGGGCTGAGAAGGTGGTACAATCCAGAATGGTTTTTGGAGAAGATACCATCATCTCCTTACGATCCCATTGTTTTACGCGAGGCTTTTGAGAAGGCTGTGTTAAAGAGACTTATGACAGATGTACCATTCGGTGTTCTTTTGTCCGGAGGATTGGACTCGTCACTCGTTGCTGCTGTGGCTTGTCGCTATTTGGCTAAATCAGAAGCTGCACGTCAGTGGGGCTCACAATTACATACCTTTTGCGTTGGCTTAGAGGGTTCTCCAGATTTAAAAGCAGCAAGAGAAGTTGCTGATTATCTTGGAACTCATCATCACGAGTTTCACTTTACTGTTCAGGAAGGCATAGATGCACTTGAAGAAGTTATCTACCATATTGAGACTTATGATGTGACCACTGTCAGAGCCAGCACTCCAATGTTTCTTATGTCTCGTAAGATAAAATCTCTGGGAGTAAAAATGGTCCTTTCTGGTGAAGGTTCAGATGAAATCTTTGGTGGCTACTTTGTATTTCCACAAGGCACCAAACAAGGAGGAGTTTCACCAAGAAACATGCCAGAAGCTCTTCACCTGTACGACTGTCTGAGGGCCAACAAATCGACTTCAGCTTGGGGTGTTGAGGCTCGAGTACCGTTTCTTGACAAAGAATTCATCAAGACTGCTATGGACATTGATCCAGAATGGAAAATGATCAGGCCTGATCTTGGGAGAATTGAGAAGTGGGTCTTGCGCAAAGCATTTGATGATGATCAGAAGCCATATTTACCAAAGCACATCTTGTACAGGCAGAAGGAGCAGTTTAGTGATGGTGTCGGTTACAGCTGGATTGACGGCTTGAAAGATCATGCCAACAAACAAAATGCTGCGAGATCGACAGTTCCCGGTGGTCCAAGTGTGGCATGCAGCACAGCAAAAGCTGTGGATTGGGATGCGGAATGGTCCAAGAACCCTGATCCATCTGGTCGTGCTGCACTTGGTATACATGCAGCTGCGTATGAGGAATCCAAAGATGTGAAGACTGGAAGTCTTGTGAGTGACTCTCCACAGAAACTTGTAAAAGGGGTTGCAGAGAAAGTGGCCGCAGTAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004066 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006082 GO:0006520 GO:0006528 GO:0006529 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009066 GO:0009067 GO:0009314 GO:0009416 GO:0009506 GO:0009628 GO:0009642 GO:0009646 GO:0009651 GO:0009987 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019752 GO:0030054 GO:0032787 GO:0034641 GO:0042538 GO:0042802 GO:0042803 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046983 GO:0050896 GO:0055044 GO:0071704 GO:0072330 GO:0097164 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00250 ko01100 ko01110 map00250 map01100 map01110

Pfam Domains

Protein Families

Asn_synthase GATase_7

Protein Analysis

579

Amino Acids

64.65

Weight (kDa)

6.39

Isoelectric Point (pI)

42.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_6	PF13522	65 - 192	1.8e-36	Glutamine amidotransferase domain
GATase_7	PF13537	80 - 197	1.1e-42	Glutamine amidotransferase domain
DUF3700	PF12481	144 - 234	2e-12	Aluminium induced protein
Asn_synthase	PF00733	242 - 389	9.6e-51	Asparagine synthase
Asn_synthase	PF00733	404 - 503	2.5e-25	Asparagine synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0000473)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10240 AT5G10240 AT5G65010 AT5G65010
fragaria_vesca	FvH4_1g20910 FvH4_1g20910 FvH4_1g29202 FvH4_3g23760 FvH4_3g42550
malus_domestica	MD01G1029700.v1.1 MD15G1324200.v1.1
prunus_persica	Prupe.6G186800_v2.0.a1 Prupe.6G186800_v2.0.a1 Prupe.6G186800_v2.0.a1
pyrus_communis	pycom01g05790
rosa_chinensis	RchiOBHm_Chr2g0113551 RchiOBHm_Chr2g0113591 RchiOBHm_Chr2g0113641 RchiOBHm_Chr2g0113651 RchiOBHm_Chr2g0113711 RchiOBHm_Chr2g0113721 RchiOBHm_Chr2g0113831 RchiOBHm_Chr2g0113841 RchiOBHm_Chr2g0113851 RchiOBHm_Chr2g0113911 RchiOBHm_Chr2g0113941 RchiOBHm_Chr2g0113991 RchiOBHm_Chr2g0114001 RchiOBHm_Chr2g0114041 RchiOBHm_Chr2g0114051 RchiOBHm_Chr2g0114061 RchiOBHm_Chr2g0114071 RchiOBHm_Chr2g0114081 RchiOBHm_Chr2g0114101 RchiOBHm_Chr2g0114131 RchiOBHm_Chr2g0114141 RchiOBHm_Chr2g0114261 RchiOBHm_Chr2g0114271 RchiOBHm_Chr2g0114291 RchiOBHm_Chr2g0124041
rosa_laevigata	RLG00000018073 RLG00000018076 RLG00000018078 RLG00000018084 RLG00000018086 RLG00000018088 RLG00000018094 RLG00000018095
rosa_multiflora	Rmu_sc0000881.1_g000007 Rmu_sc0000929.1_g000008 Rmu_sc0000929.1_g000017 Rmu_sc0000929.1_g000029 Rmu_sc0000929.1_g000039 Rmu_sc0001699.1_g000005 Rmu_sc0004283.1_g000004 Rmu_sc0007010.1_g000001 Rmu_sc0007415.1_g000028 Rmu_sc0007415.1_g000045 Rmu_sc0009639.1_g000009 Rmu_sc0010543.1_g000008 Rmu_sc0011941.1_g000022 Rmu_sc0018769.1_g000001
rosa_roxburghii	Rroxscaffold_2G00129150 Rroxscaffold_2G00130050 Rroxscaffold_2G00130070 Rroxscaffold_2G00130090
rosa_rugosa	Rorug02G0188100 Rorug02G0188100 Rorug02G0189700 Rorug02G0190000 Rorug02G0190000 Rorug03G0301100
rosa_samantha	Rh2AG242700 Rh2AG243100 Rh2AG245300 Rh2AG246800 Rh2BG256600 Rh2DG253800 Rh6CG172600
rosa_wichuraiana	Rw0G014630 Rw2G018450 Rw2G018500 Rw2G018980 Rw2G019030 Rw2G019050 Rw2G019100 Rw2G019130 Rw2G019190 Rw2G019420 Rw5G028430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1379
Acc65I	GGTACC	1 cut(s)	80
AccB1I	GGYRCC	2 cut(s)	80, 1169
AccBSI	CCGCTC	2 cut(s)	204, 283
AccI	GTMKAC	1 cut(s)	1627
AccII	CGCG	2 cut(s)	189, 729
AccIII	TCCGGA	1 cut(s)	794
AciI	CCGC	9 cut(s)	43, 120, 123, 181, 189, 204, 281, 1580, 1728
AclWI	GGATC	7 cut(s)	11, 104, 282, 562, 707, 1316, 1595
AcoI	YGGCCR	1 cut(s)	1725
AcsI	RAATTY	2 cut(s)	431, 1295
AcuI	CTGAAG	2 cut(s)	555, 1236
AfaI	GTAC	7 cut(s)	82, 155, 668, 774, 1223, 1278, 1430
AfiI	CCNNNNNNNGG	3 cut(s)	269, 415, 1352
AgsI	TTSAA	3 cut(s)	361, 1007, 1486
AhdI	GACNNNNNGTC	1 cut(s)	1671
AjiI	CACGTC	1 cut(s)	863
AleI	CACNNNNGTG	1 cut(s)	865
AloI	GAACNNNNNNTCC	2 cut(s)	440, 472
AluBI	AGCT	8 cut(s)	58, 584, 857, 1211, 1256, 1470, 1565, 1637
AluI	AGCT	8 cut(s)	58, 584, 857, 1211, 1256, 1470, 1565, 1637
Alw21I	GWGCWC	1 cut(s)	297
Alw26I	GTCTC	4 cut(s)	477, 751, 1022, 1083
AlwI	GGATC	7 cut(s)	11, 104, 282, 562, 707, 1316, 1595
AlwNI	CAGNNNCTG	2 cut(s)	390, 857
Ama87I	CYCGRG	4 cut(s)	142, 504, 623, 1272
Aor13HI	TCCGGA	1 cut(s)	794
AoxI	GGCC	3 cut(s)	1236, 1343, 1725
ApoI	RAATTY	2 cut(s)	431, 1295
Asp700I	GAANNNNTTC	1 cut(s)	1212
Asp718I	GGTACC	1 cut(s)	80
AspLEI	GCGC	2 cut(s)	406, 1380
AspS9I	GGNCC	6 cut(s)	191, 259, 1111, 1236, 1535, 1587
AsuC2I	CCSGG	5 cut(s)	143, 144, 624, 625, 1530
AsuHPI	GGTGA	4 cut(s)	335, 1133, 1182, 1208
AvaI	CYCGRG	4 cut(s)	142, 504, 623, 1272
AvaII	GGWCC	5 cut(s)	191, 259, 1111, 1535, 1587
AxyI	CCTNAGG	1 cut(s)	268
BaeI	ACNNNNGTAYC	2 cut(s)	756, 789
BanI	GGYRCC	2 cut(s)	80, 1169
BanII	GRGCYC	1 cut(s)	875
BauI	CACGAG	1 cut(s)	965
BbsI	GAAGAC	2 cut(s)	94, 1670
Bbv12I	GWGCWC	1 cut(s)	297
BccI	CCATC	5 cut(s)	168, 545, 706, 1448, 1612
BceAI	ACGGC	1 cut(s)	1495
BcgI	CGANNNNNNTGC	2 cut(s)	98, 132
BclI	TGATCA	2 cut(s)	1338, 1396
BcnI	CCSGG	5 cut(s)	143, 144, 624, 625, 1530
BcoDI	GTCTC	4 cut(s)	477, 751, 1022, 1083
BfaI	CTAG	1 cut(s)	639
Bme1390I	CCNGG	5 cut(s)	143, 144, 624, 625, 1530
Bme18I	GGWCC	5 cut(s)	191, 259, 1111, 1535, 1587
BmeRI	GACNNNNNGTC	1 cut(s)	1671
BmeT110I	CYCGRG	4 cut(s)	142, 504, 623, 1272
BmgBI	CACGTC	1 cut(s)	863
BmgT120I	GGNCC	6 cut(s)	191, 259, 1111, 1236, 1535, 1587
BmiI	GGNNCC	4 cut(s)	82, 164, 261, 1171
BmrFI	CCNGG	5 cut(s)	143, 144, 624, 625, 1530
BmsI	GCATC	5 cut(s)	193, 499, 637, 988, 1567
BoxI	GACNNNNGTC	1 cut(s)	1046
BpiI	GAAGAC	2 cut(s)	94, 1670
BplI	GAGNNNNNCTC	2 cut(s)	1672, 1704
BpmI	CTGGAG	1 cut(s)	897
BpuMI	CCSGG	5 cut(s)	143, 144, 624, 625, 1530
Bsa29I	ATCGAT	1 cut(s)	370
BsaBI	GATNNNNATC	2 cut(s)	610, 1395
BsaJI	CCNNGG	5 cut(s)	141, 142, 143, 217, 623
BsaWI	WCCGGW	2 cut(s)	83, 794
Bsc4I	CCNNNNNNNGG	3 cut(s)	269, 415, 1352
Bse1I	ACTGG	2 cut(s)	385, 1672
Bse21I	CCTNAGG	1 cut(s)	268
Bse3DI	GCAATG	1 cut(s)	211
Bse8I	GATNNNNATC	2 cut(s)	610, 1395
BseAI	TCCGGA	1 cut(s)	794
BseCI	ATCGAT	1 cut(s)	370
BseDI	CCNNGG	5 cut(s)	141, 142, 143, 217, 623
BseGI	GGATG	2 cut(s)	459, 1582
BseJI	GATNNNNATC	2 cut(s)	610, 1395
BseLI	CCNNNNNNNGG	3 cut(s)	269, 415, 1352
BseMI	GCAATG	1 cut(s)	211
BseMII	CTCAG	3 cut(s)	282, 647, 1223
BseNI	ACTGG	2 cut(s)	385, 1672
BseRI	GAGGAG	1 cut(s)	1197
BsgI	GTGCAG	3 cut(s)	31, 843, 1602
Bsh1236I	CGCG	2 cut(s)	189, 729
Bsh1285I	CGRYCG	1 cut(s)	9
BshFI	GGCC	3 cut(s)	1238, 1345, 1727
BshNI	GGYRCC	2 cut(s)	80, 1169
BshVI	ATCGAT	1 cut(s)	370
BsiEI	CGRYCG	1 cut(s)	9
BsiHKAI	GWGCWC	1 cut(s)	297
BsiHKCI	CYCGRG	4 cut(s)	142, 504, 623, 1272
BsiSI	CCGG	5 cut(s)	84, 143, 624, 795, 1530
BslI	CCNNNNNNNGG	3 cut(s)	269, 415, 1352
BsmAI	GTCTC	4 cut(s)	477, 751, 1022, 1083
BsmI	GAATGC	1 cut(s)	37
BsnI	GGCC	3 cut(s)	1238, 1345, 1727
BsoBI	CYCGRG	4 cut(s)	142, 504, 623, 1272
Bsp1286I	GDGCHC	2 cut(s)	297, 875
Bsp13I	TCCGGA	1 cut(s)	794
Bsp1407I	TGTACA	1 cut(s)	1428
Bsp19I	CCATGG	1 cut(s)	217
BspACI	CCGC	9 cut(s)	43, 120, 123, 181, 189, 204, 281, 1580, 1728
BspANI	GGCC	3 cut(s)	1238, 1345, 1727
BspCNI	CTCAG	3 cut(s)	281, 648, 1224
BspDI	ATCGAT	1 cut(s)	370
BspEI	TCCGGA	1 cut(s)	794
BspFNI	CGCG	2 cut(s)	189, 729
BspLI	GGNNCC	4 cut(s)	82, 164, 261, 1171
BspPI	GGATC	7 cut(s)	11, 104, 282, 562, 707, 1316, 1595
BspQI	GCTCTTC	1 cut(s)	1218
BspT107I	GGYRCC	2 cut(s)	80, 1169
BsrBI	CCGCTC	2 cut(s)	204, 283
BsrDI	GCAATG	1 cut(s)	211
BsrGI	TGTACA	1 cut(s)	1428
BsrI	ACTGG	2 cut(s)	385, 1672
BssECI	CCNNGG	5 cut(s)	141, 142, 143, 217, 623
BssNAI	GTATAC	1 cut(s)	1628
BssSI	CACGAG	1 cut(s)	965
BssT1I	CCWWGG	1 cut(s)	217
Bst1107I	GTATAC	1 cut(s)	1628
Bst2BI	CACGAG	1 cut(s)	965
Bst4CI	ACNGT	9 cut(s)	80, 304, 316, 392, 982, 1048, 1229, 1281, 1525
Bst6I	CTCTTC	1 cut(s)	1218
BstAUI	TGTACA	1 cut(s)	1428
BstC8I	GCNNGC	3 cut(s)	56, 1057, 1549
BstDEI	CTNAG	4 cut(s)	268, 656, 901, 1232
BstDSI	CCRYGG	1 cut(s)	217
BstENI	CCTNNNNNAGG	2 cut(s)	267, 413
BstF5I	GGATG	2 cut(s)	459, 1582
BstFNI	CGCG	2 cut(s)	189, 729
BstHHI	GCGC	2 cut(s)	406, 1380
BstMAI	GTCTC	4 cut(s)	477, 751, 1022, 1083
BstMCI	CGRYCG	1 cut(s)	9
BstMWI	GCNNNNNNNGC	4 cut(s)	51, 509, 827, 1441
BstNSI	RCATGY	4 cut(s)	445, 1204, 1551, 1634
BstPAI	GACNNNNGTC	1 cut(s)	1046
BstSCI	CCNGG	5 cut(s)	141, 142, 622, 623, 1528
BstUI	CGCG	2 cut(s)	189, 729
BstV2I	GAAGAC	2 cut(s)	94, 1670
BstZ17I	GTATAC	1 cut(s)	1628
Bsu15I	ATCGAT	1 cut(s)	370
Bsu36I	CCTNAGG	1 cut(s)	268
BsuRI	GGCC	3 cut(s)	1238, 1345, 1727
BsuTUI	ATCGAT	1 cut(s)	370
BtgI	CCRYGG	1 cut(s)	217
BtgZI	GCGATG	1 cut(s)	170
BtrI	CACGTC	1 cut(s)	863
BtsCI	GGATG	2 cut(s)	459, 1582
BtsI	GCAGTG	1 cut(s)	391
BtsIMutI	CAGTG	3 cut(s)	391, 872, 1044
Cac8I	GCNNGC	3 cut(s)	56, 1057, 1549
CaiI	CAGNNNCTG	2 cut(s)	390, 857
CfoI	GCGC	2 cut(s)	406, 1380
Cfr13I	GGNCC	6 cut(s)	191, 259, 1111, 1236, 1535, 1587
Cfr9I	CCCGGG	2 cut(s)	142, 623
ClaI	ATCGAT	1 cut(s)	370
CseI	GACGC	1 cut(s)	107
Csp6I	GTAC	7 cut(s)	81, 154, 667, 773, 1222, 1277, 1429
CspCI	CAANNNNNGTGG	2 cut(s)	1548, 1583
CviAII	CATG	9 cut(s)	218, 422, 442, 496, 535, 1201, 1493, 1548, 1631
CviQI	GTAC	7 cut(s)	81, 154, 667, 773, 1222, 1277, 1429
DdeI	CTNAG	4 cut(s)	268, 656, 901, 1232
DraI	TTTAAA	1 cut(s)	921
DriI	GACNNNNNGTC	1 cut(s)	1671
EaeI	YGGCCR	1 cut(s)	1725
Eam1104I	CTCTTC	1 cut(s)	1218
Eam1105I	GACNNNNNGTC	1 cut(s)	1671
EarI	CTCTTC	1 cut(s)	1218
Eco130I	CCWWGG	1 cut(s)	217
Eco147I	AGGCCT	1 cut(s)	1345
Eco24I	GRGCYC	1 cut(s)	875
Eco47I	GGWCC	5 cut(s)	191, 259, 1111, 1535, 1587
Eco57I	CTGAAG	2 cut(s)	555, 1236
Eco81I	CCTNAGG	1 cut(s)	268
Eco88I	CYCGRG	4 cut(s)	142, 504, 623, 1272
EcoNI	CCTNNNNNAGG	2 cut(s)	267, 413
EcoRI	GAATTC	1 cut(s)	1295
EcoT14I	CCWWGG	1 cut(s)	217
EcoT38I	GRGCYC	1 cut(s)	875
ErhI	CCWWGG	1 cut(s)	217
FaeI	CATG	9 cut(s)	221, 425, 445, 499, 538, 1204, 1496, 1551, 1634
FalI	AAGNNNNNCTT	4 cut(s)	1358, 1390, 1409, 1441
FatI	CATG	9 cut(s)	217, 421, 441, 495, 534, 1200, 1492, 1547, 1630
FauI	CCCGC	1 cut(s)	197
FbaI	TGATCA	2 cut(s)	1338, 1396
FblI	GTMKAC	1 cut(s)	1627
FokI	GGATG	2 cut(s)	466, 1589
FriOI	GRGCYC	1 cut(s)	875
FspBI	CTAG	1 cut(s)	639
FspI	TGCGCA	1 cut(s)	1379
GlaI	GCGC	2 cut(s)	405, 1379
GsuI	CTGGAG	1 cut(s)	897
HaeIII	GGCC	3 cut(s)	1238, 1345, 1727
HapII	CCGG	5 cut(s)	84, 143, 624, 795, 1530
HgaI	GACGC	1 cut(s)	107
HhaI	GCGC	2 cut(s)	406, 1380
Hin1II	CATG	9 cut(s)	221, 425, 445, 499, 538, 1204, 1496, 1551, 1634
Hin6I	GCGC	2 cut(s)	404, 1378
HinP1I	GCGC	2 cut(s)	404, 1378
HincII	GTYRAC	1 cut(s)	439
HindII	GTYRAC	1 cut(s)	439
HindIII	AAGCTT	1 cut(s)	56
HinfI	GANTC	5 cut(s)	372, 606, 806, 1649, 1685
HpaII	CCGG	5 cut(s)	84, 143, 624, 795, 1530
HphI	GGTGA	4 cut(s)	335, 1133, 1182, 1208
Hpy166II	GTNNAC	4 cut(s)	156, 259, 439, 1628
Hpy188I	TCNGA	9 cut(s)	28, 167, 377, 574, 853, 1052, 1132, 1233, 1401
Hpy8I	GTNNAC	4 cut(s)	156, 259, 439, 1628
Hpy99I	CGWCG	1 cut(s)	101
HpyAV	CCTTC	7 cut(s)	103, 355, 654, 736, 983, 1118, 1432
HpyCH4III	ACNGT	9 cut(s)	80, 304, 316, 392, 982, 1048, 1229, 1281, 1525
HpyCH4IV	ACGT	1 cut(s)	862
HpyCH4V	TGCA	8 cut(s)	48, 211, 860, 1001, 1551, 1619, 1634, 1715
HpyF10VI	GCNNNNNNNGC	4 cut(s)	51, 509, 827, 1441
HpyF3I	CTNAG	4 cut(s)	268, 656, 901, 1232
HpySE526I	ACGT	1 cut(s)	862
Hsp92II	CATG	9 cut(s)	221, 425, 445, 499, 538, 1204, 1496, 1551, 1634
HspAI	GCGC	2 cut(s)	404, 1378
Kpn2I	TCCGGA	1 cut(s)	794
KpnI	GGTACC	1 cut(s)	84
Ksp22I	TGATCA	2 cut(s)	1338, 1396
LguI	GCTCTTC	1 cut(s)	1218
LmnI	GCTCC	4 cut(s)	40, 168, 201, 1441
LweI	GCATC	5 cut(s)	193, 499, 637, 988, 1567
MaeI	CTAG	1 cut(s)	639
MaeII	ACGT	1 cut(s)	862
MaeIII	GTNAC	7 cut(s)	227, 310, 386, 810, 1039, 1463, 1682
MbiI	CCGCTC	2 cut(s)	204, 283
MboII	GAAGA	6 cut(s)	99, 440, 703, 1019, 1205, 1675
MfeI	CAATTG	1 cut(s)	114
MhlI	GDGCHC	2 cut(s)	297, 875
MluCI	AATT	6 cut(s)	114, 431, 558, 878, 1295, 1359
MlyI	GAGTC	2 cut(s)	800, 1679
MroI	TCCGGA	1 cut(s)	794
MroXI	GAANNNNTTC	1 cut(s)	1212
MseI	TTAA	3 cut(s)	318, 752, 920
MslI	CAYNNNNRTG	4 cut(s)	216, 494, 865, 1064
MspA1I	CMGCKG	3 cut(s)	45, 1470, 1637
MspI	CCGG	5 cut(s)	84, 143, 624, 795, 1530
MspR9I	CCNGG	5 cut(s)	143, 144, 624, 625, 1530
MunI	CAATTG	1 cut(s)	114
Mva1269I	GAATGC	1 cut(s)	37
MvnI	CGCG	2 cut(s)	189, 729
MwoI	GCNNNNNNNGC	4 cut(s)	51, 509, 827, 1441
NciI	CCSGG	5 cut(s)	143, 144, 624, 625, 1530
NcoI	CCATGG	1 cut(s)	217
NlaIII	CATG	9 cut(s)	221, 425, 445, 499, 538, 1204, 1496, 1551, 1634
NlaIV	GGNNCC	4 cut(s)	82, 164, 261, 1171
NmeAIII	GCCGAG	1 cut(s)	139
NmuCI	GTSAC	5 cut(s)	310, 386, 810, 1039, 1682
NsbI	TGCGCA	1 cut(s)	1379
NspI	RCATGY	4 cut(s)	445, 1204, 1551, 1634
OliI	CACNNNNGTG	1 cut(s)	865
PaeI	GCATGC	1 cut(s)	1551
PaeR7I	CTCGAG	2 cut(s)	504, 1272
PceI	AGGCCT	1 cut(s)	1345
PciSI	GCTCTTC	1 cut(s)	1218
PcsI	WCGNNNNNNNCGW	1 cut(s)	105
PctI	GAATGC	1 cut(s)	37
PdmI	GAANNNNTTC	1 cut(s)	1212
PfeI	GAWTC	3 cut(s)	372, 606, 1649
PleI	GAGTC	2 cut(s)	800, 1679
PpsI	GAGTC	2 cut(s)	800, 1679
PshAI	GACNNNNGTC	1 cut(s)	1046
PspN4I	GGNNCC	4 cut(s)	82, 164, 261, 1171
PspPI	GGNCC	6 cut(s)	191, 259, 1111, 1236, 1535, 1587
PspXI	VCTCGAGB	1 cut(s)	1272
PstNI	CAGNNNCTG	2 cut(s)	390, 857
PvuII	CAGCTG	2 cut(s)	1470, 1637
RsaI	GTAC	7 cut(s)	82, 155, 668, 774, 1223, 1278, 1430
RsaNI	GTAC	7 cut(s)	81, 154, 667, 773, 1222, 1277, 1429
RseI	CAYNNNNRTG	4 cut(s)	216, 494, 865, 1064
SapI	GCTCTTC	1 cut(s)	1218
SaqAI	TTAA	3 cut(s)	318, 752, 920
Sau96I	GGNCC	6 cut(s)	191, 259, 1111, 1236, 1535, 1587
SchI	GAGTC	2 cut(s)	800, 1679
ScrFI	CCNGG	5 cut(s)	143, 144, 624, 625, 1530
SduI	GDGCHC	2 cut(s)	297, 875
SfaNI	GCATC	5 cut(s)	193, 499, 637, 988, 1567
Sfr274I	CTCGAG	2 cut(s)	504, 1272
SinI	GGWCC	5 cut(s)	191, 259, 1111, 1535, 1587
SlaI	CTCGAG	2 cut(s)	504, 1272
SmaI	CCCGGG	2 cut(s)	144, 625
SmiMI	CAYNNNNRTG	4 cut(s)	216, 494, 865, 1064
SmlI	CTYRAG	2 cut(s)	504, 1272
SmoI	CTYRAG	2 cut(s)	504, 1272
SphI	GCATGC	1 cut(s)	1551
Sse9I	AATT	6 cut(s)	114, 431, 558, 878, 1295, 1359
SseBI	AGGCCT	1 cut(s)	1345
SsiI	CCGC	9 cut(s)	43, 120, 123, 181, 189, 204, 281, 1580, 1728
SspMI	CTAG	1 cut(s)	639
StuI	AGGCCT	1 cut(s)	1345
StyD4I	CCNGG	5 cut(s)	141, 142, 622, 623, 1528
StyI	CCWWGG	1 cut(s)	217
TaaI	ACNGT	9 cut(s)	80, 304, 316, 392, 982, 1048, 1229, 1281, 1525
TaiI	ACGT	1 cut(s)	865
TaqI	TCGA	6 cut(s)	96, 370, 505, 1247, 1273, 1520
TasI	AATT	6 cut(s)	114, 431, 558, 878, 1295, 1359
TatI	WGTACW	1 cut(s)	1428
TauI	GCSGC	5 cut(s)	122, 125, 184, 283, 1730
TfiI	GAWTC	3 cut(s)	372, 606, 1649
Tru1I	TTAA	3 cut(s)	318, 752, 920
Tru9I	TTAA	3 cut(s)	318, 752, 920
TscAI	CASTG	3 cut(s)	391, 872, 1051
TseFI	GTSAC	5 cut(s)	310, 386, 810, 1039, 1682
Tsp45I	GTSAC	5 cut(s)	310, 386, 810, 1039, 1682
TspDTI	ATGAA	5 cut(s)	484, 593, 598, 1149, 1288
TspMI	CCCGGG	2 cut(s)	142, 623
TspRI	CASTG	3 cut(s)	391, 872, 1051
VpaK11BI	GGWCC	5 cut(s)	191, 259, 1111, 1535, 1587
XagI	CCTNNNNNAGG	2 cut(s)	267, 413
XapI	RAATTY	2 cut(s)	431, 1295
XceI	RCATGY	4 cut(s)	445, 1204, 1551, 1634
XhoI	CTCGAG	2 cut(s)	504, 1272
XmaI	CCCGGG	2 cut(s)	142, 623
XmiI	GTMKAC	1 cut(s)	1627
XmnI	GAANNNNTTC	1 cut(s)	1212
XspI	CTAG	1 cut(s)	639

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.