Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0000473

Physical Location & Seq

Genomic Coordinates Forward (+)

24906064 .. 24912896

Gene Structure 6833 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G018980.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1725 bp

ATGTGTGGAATTCTCGCCGTCTTGGGCTGCGCCGACAACTCTCAGGCCAAGCGGTCTCGCATTATCGAACTCTCGCGCCGATTGCGGCATCGCGGTCCTGATTGGAGCGGGTTGCATTGCCATGGAGATTGTTACCTTGCTCATCAGCGATTGGCTATTGTGGACCCTGCCTCAGGGGATCAGCCGCTCTACAACGAGGACAAGACCGTCATTGTCACGGTTAATGGTGAGATATATAACCACAAGCAGTTGAGAGAAACATTGAAGGGTCATCGATTCCGAACTGGCAGTGACTGTGAAGTGATAGCACATCTTTACGAGGAACATGGGGAAGAATTTGTTGACATGCTGGACGGGATGTTCTCCTTTGTCCTCCTTGATACAAGAGACAACACTTTCATGGCGGCTCGAGATGCTATTGGCATTACCCCACTTTACATGGGTTGGGGTCTTGATGGATCAATTTGGTTTGCTTCAGAAATGAAAGCTATAAGTGATGATTGTGAAAGATTCATATCTTTTCCTCCTGGGCATCTATACTCTAGCAAACAAGGAGGGCTGAGAAGGTGGTACAATCCAGAATGGTTTTTGGAGAAGATACCATCATCTCCTTACGATCCCATTGTTTTACGCGAGGCTTTTGAGAAGGCTGTGTTAAAGAGACTTATGACAGATGTACCATTCGGTGTTCTTTTGTCCGGAGGATTGGACTCGTCACTCGTTGCTGCTGTGGCTTGTCACTATTTGGCTAAATCAGATGCTGCACGTCAGTGGGGGTCACAATTACATACCTTTTCGATAACTGAATATTTAAAAGCAGCAAGAGAAGTTGCTGATTATCTTGGAACTCATCATCACGAGTTTCACTTTACTGTTCAGGAAGGCATAGATGCACTTGAAGAAGTTATCTACCATATTGAGACTTATGATGTGACCACTGTCAGAGCCAGCACTCCAATGTTTCTTATGTCTCGTAAGATAAAATCTCTGGGAGTAAAAATGGTCCTTTCTGGTGAAGGTTCAGATGAAATCTTTGGTGGCTACTTGTATTTCCACAAGGCACCAAACAAGGAGGAGTTTCACCAAGAAACATGCCAGAAGATGAAAGCTCTTCACCTGTACGACTGTCTGAGGGCCAACAAATCGACTTCAGCTTGGGGTGTTGAGGCTCGAGTACCGTTTCTTGACAAAGAATTCATCAAGACTGCTATGGACATTGATCCAGAATGGAAAATGATCAGGCCTGATCTTGGGAGAATTGAGAAGTGGGTCTTGCGCAAAGCATTTGATGATGATCAGAAGCCATATTTACCAAAGCACATCTTGTACAGGCAGAAGGAGCAGTTTAGTGATGGTGTCGGTTACAGCTGGATTGACGGCTTGAAAGATCATGCCAACAAACAAGTAACAGATTCAATGCTGATGAACGCAAGCTTTGTCTACCCTGAAAACACTCCAACTACAAAAGAAGGATACTGCTATAGGACTATCTTTGAGAAATATTTTCCAAAGAATGCTGCGAGATCGACAGTTCCCGGTGGTCCAAGTGTGGCATGCAGCACAGCAAAAGCTGTGGAGTGGGATGCGGAATGGTCCAAGAACCCTGATCCATCTGGTCGTGCTGCACTTGGTATACATGCAGCTGCGTATGAGGAATCCAAAGATGTGAAGACTGGAAATCTTGTGAGTGACTCTCCACAGAAACTTGTAAAAGTGCAGCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004066 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006082 GO:0006520 GO:0006528 GO:0006529 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009066 GO:0009067 GO:0009314 GO:0009416 GO:0009506 GO:0009628 GO:0009642 GO:0009646 GO:0009651 GO:0009987 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019752 GO:0030054 GO:0032787 GO:0034641 GO:0042538 GO:0042802 GO:0042803 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046983 GO:0050896 GO:0055044 GO:0071704 GO:0072330 GO:0097164 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00250 ko01100 ko01110 map00250 map01100 map01110

Pfam Domains

Protein Families

Asn_synthase GATase_7

Protein Analysis

574

Amino Acids

65.01

Weight (kDa)

6.14

Isoelectric Point (pI)

43.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_6	PF13522	33 - 160	4.9e-37	Glutamine amidotransferase domain
GATase_7	PF13537	48 - 165	3.7e-43	Glutamine amidotransferase domain
DUF3700	PF12481	112 - 202	2e-12	Aluminium induced protein
Asn_synthase	PF00733	210 - 357	6.8e-47	Asparagine synthase
Asn_synthase	PF00733	355 - 507	1.8e-32	Asparagine synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0000473)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10240 AT5G10240 AT5G65010 AT5G65010
fragaria_vesca	FvH4_1g20910 FvH4_1g20910 FvH4_1g29202 FvH4_3g23760 FvH4_3g42550
malus_domestica	MD01G1029700.v1.1 MD15G1324200.v1.1
prunus_persica	Prupe.6G186800_v2.0.a1 Prupe.6G186800_v2.0.a1 Prupe.6G186800_v2.0.a1
pyrus_communis	pycom01g05790
rosa_chinensis	RchiOBHm_Chr2g0113551 RchiOBHm_Chr2g0113591 RchiOBHm_Chr2g0113641 RchiOBHm_Chr2g0113651 RchiOBHm_Chr2g0113711 RchiOBHm_Chr2g0113721 RchiOBHm_Chr2g0113831 RchiOBHm_Chr2g0113841 RchiOBHm_Chr2g0113851 RchiOBHm_Chr2g0113911 RchiOBHm_Chr2g0113941 RchiOBHm_Chr2g0113991 RchiOBHm_Chr2g0114001 RchiOBHm_Chr2g0114041 RchiOBHm_Chr2g0114051 RchiOBHm_Chr2g0114061 RchiOBHm_Chr2g0114071 RchiOBHm_Chr2g0114081 RchiOBHm_Chr2g0114101 RchiOBHm_Chr2g0114131 RchiOBHm_Chr2g0114141 RchiOBHm_Chr2g0114261 RchiOBHm_Chr2g0114271 RchiOBHm_Chr2g0114291 RchiOBHm_Chr2g0124041
rosa_laevigata	RLG00000018073 RLG00000018076 RLG00000018078 RLG00000018084 RLG00000018086 RLG00000018088 RLG00000018094 RLG00000018095
rosa_multiflora	Rmu_sc0000881.1_g000007 Rmu_sc0000929.1_g000008 Rmu_sc0000929.1_g000017 Rmu_sc0000929.1_g000029 Rmu_sc0000929.1_g000039 Rmu_sc0001699.1_g000005 Rmu_sc0004283.1_g000004 Rmu_sc0007010.1_g000001 Rmu_sc0007415.1_g000028 Rmu_sc0007415.1_g000045 Rmu_sc0009639.1_g000009 Rmu_sc0010543.1_g000008 Rmu_sc0011941.1_g000022 Rmu_sc0018769.1_g000001
rosa_roxburghii	Rroxscaffold_2G00129150 Rroxscaffold_2G00130050 Rroxscaffold_2G00130070 Rroxscaffold_2G00130090
rosa_rugosa	Rorug02G0188100 Rorug02G0188100 Rorug02G0189700 Rorug02G0190000 Rorug02G0190000 Rorug03G0301100
rosa_samantha	Rh2AG242700 Rh2AG243100 Rh2AG245300 Rh2AG246800 Rh2BG256600 Rh2DG253800 Rh6CG172600
rosa_wichuraiana	Rw0G014630 Rw2G018450 Rw2G018500 Rw2G018980 Rw2G019030 Rw2G019050 Rw2G019100 Rw2G019130 Rw2G019190 Rw2G019420 Rw5G028430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	206
Acc16I	TGCGCA	1 cut(s)	1277
AccB1I	GGYRCC	1 cut(s)	1060
AccBSI	CCGCTC	2 cut(s)	108, 187
AccI	GTMKAC	2 cut(s)	1440, 1633
AccII	CGCG	3 cut(s)	76, 93, 633
AccIII	TCCGGA	1 cut(s)	698
AciI	CCGC	7 cut(s)	52, 85, 93, 108, 185, 404, 1586
AclWI	GGATC	5 cut(s)	186, 466, 611, 1214, 1601
AcsI	RAATTY	3 cut(s)	9, 335, 1193
AcuI	CTGAAG	2 cut(s)	459, 1134
AfaI	GTAC	5 cut(s)	572, 678, 1121, 1176, 1328
AfiI	CCNNNNNNNGG	2 cut(s)	173, 1250
AgsI	TTSAA	4 cut(s)	265, 899, 1384, 1416
AjiI	CACGTC	1 cut(s)	767
AjnI	CCWGG	1 cut(s)	526
AleI	CACNNNNGTG	1 cut(s)	769
AloI	GAACNNNNNNTCC	2 cut(s)	344, 376
AluBI	AGCT	7 cut(s)	488, 1109, 1154, 1368, 1434, 1571, 1643
AluI	AGCT	7 cut(s)	488, 1109, 1154, 1368, 1434, 1571, 1643
Alw26I	GTCTC	5 cut(s)	60, 381, 655, 914, 975
AlwI	GGATC	5 cut(s)	186, 466, 611, 1214, 1601
AlwNI	CAGNNNCTG	2 cut(s)	294, 761
Ama87I	CYCGRG	2 cut(s)	408, 1170
Aor13HI	TCCGGA	1 cut(s)	698
AoxI	GGCC	3 cut(s)	45, 1134, 1241
ApoI	RAATTY	3 cut(s)	9, 335, 1193
AspLEI	GCGC	3 cut(s)	32, 78, 1278
AspS9I	GGNCC	6 cut(s)	95, 163, 1003, 1134, 1541, 1593
AsuC2I	CCSGG	1 cut(s)	1536
AsuHPI	GGTGA	4 cut(s)	239, 1025, 1073, 1106
AvaI	CYCGRG	2 cut(s)	408, 1170
AvaII	GGWCC	5 cut(s)	95, 163, 1003, 1541, 1593
AxyI	CCTNAGG	1 cut(s)	172
BaeI	ACNNNNGTAYC	2 cut(s)	660, 693
BanI	GGYRCC	1 cut(s)	1060
BauI	CACGAG	1 cut(s)	857
BbsI	GAAGAC	1 cut(s)	1676
BbvI	GCAGC	9 cut(s)	14, 712, 748, 830, 1504, 1569, 1609, 1630, 1652
BccI	CCATC	4 cut(s)	449, 610, 1346, 1618
BceAI	ACGGC	2 cut(s)	2, 1393
BciT130I	CCWGG	1 cut(s)	528
BciVI	GTATCC	1 cut(s)	1466
BclI	TGATCA	2 cut(s)	1236, 1294
BcnI	CCSGG	1 cut(s)	1536
BcoDI	GTCTC	5 cut(s)	60, 381, 655, 914, 975
BfaI	CTAG	1 cut(s)	543
BfmI	CTRYAG	1 cut(s)	1480
BfuI	GTATCC	1 cut(s)	1466
Bme1390I	CCNGG	2 cut(s)	528, 1536
Bme18I	GGWCC	5 cut(s)	95, 163, 1003, 1541, 1593
BmeT110I	CYCGRG	2 cut(s)	408, 1170
BmgBI	CACGTC	1 cut(s)	767
BmgT120I	GGNCC	6 cut(s)	95, 163, 1003, 1134, 1541, 1593
BmiI	GGNNCC	2 cut(s)	165, 1062
BmrFI	CCNGG	2 cut(s)	528, 1536
BmsI	GCATC	6 cut(s)	97, 403, 541, 748, 880, 1573
BoxI	GACNNNNGTC	1 cut(s)	938
BpiI	GAAGAC	1 cut(s)	1676
BplI	GAGNNNNNCTC	2 cut(s)	1678, 1710
BpuMI	CCSGG	1 cut(s)	1536
Bsa29I	ATCGAT	1 cut(s)	274
BsaBI	GATNNNNATC	2 cut(s)	514, 1293
BsaI	GGTCTC	1 cut(s)	60
BsaJI	CCNNGG	2 cut(s)	121, 527
BsaWI	WCCGGW	1 cut(s)	698
Bsc4I	CCNNNNNNNGG	2 cut(s)	173, 1250
Bse1I	ACTGG	2 cut(s)	289, 1678
Bse21I	CCTNAGG	1 cut(s)	172
Bse3DI	GCAATG	1 cut(s)	115
Bse8I	GATNNNNATC	2 cut(s)	514, 1293
BseAI	TCCGGA	1 cut(s)	698
BseBI	CCWGG	1 cut(s)	528
BseCI	ATCGAT	1 cut(s)	274
BseDI	CCNNGG	2 cut(s)	121, 527
BseGI	GGATG	2 cut(s)	363, 1588
BseJI	GATNNNNATC	2 cut(s)	514, 1293
BseLI	CCNNNNNNNGG	2 cut(s)	173, 1250
BseMI	GCAATG	1 cut(s)	115
BseMII	CTCAG	4 cut(s)	56, 186, 551, 1121
BseNI	ACTGG	2 cut(s)	289, 1678
BseRI	GAGGAG	1 cut(s)	1088
BseXI	GCAGC	9 cut(s)	14, 712, 748, 830, 1504, 1569, 1609, 1630, 1652
BsgI	GTGCAG	2 cut(s)	747, 1608
Bsh1236I	CGCG	3 cut(s)	76, 93, 633
BshFI	GGCC	3 cut(s)	47, 1136, 1243
BshNI	GGYRCC	1 cut(s)	1060
BshVI	ATCGAT	1 cut(s)	274
BsiHKCI	CYCGRG	2 cut(s)	408, 1170
BsiSI	CCGG	2 cut(s)	699, 1536
BslI	CCNNNNNNNGG	2 cut(s)	173, 1250
BsmAI	GTCTC	5 cut(s)	60, 381, 655, 914, 975
BsmI	GAATGC	1 cut(s)	1519
BsnI	GGCC	3 cut(s)	47, 1136, 1243
Bso31I	GGTCTC	1 cut(s)	60
BsoBI	CYCGRG	2 cut(s)	408, 1170
Bsp13I	TCCGGA	1 cut(s)	698
Bsp1407I	TGTACA	1 cut(s)	1326
Bsp19I	CCATGG	1 cut(s)	121
BspACI	CCGC	7 cut(s)	52, 85, 93, 108, 185, 404, 1586
BspANI	GGCC	3 cut(s)	47, 1136, 1243
BspCNI	CTCAG	4 cut(s)	55, 185, 552, 1122
BspDI	ATCGAT	1 cut(s)	274
BspEI	TCCGGA	1 cut(s)	698
BspFNI	CGCG	3 cut(s)	76, 93, 633
BspLI	GGNNCC	2 cut(s)	165, 1062
BspPI	GGATC	5 cut(s)	186, 466, 611, 1214, 1601
BspQI	GCTCTTC	1 cut(s)	1116
BspT107I	GGYRCC	1 cut(s)	1060
BspTNI	GGTCTC	1 cut(s)	60
BsrBI	CCGCTC	2 cut(s)	108, 187
BsrDI	GCAATG	1 cut(s)	115
BsrGI	TGTACA	1 cut(s)	1326
BsrI	ACTGG	2 cut(s)	289, 1678
BssECI	CCNNGG	2 cut(s)	121, 527
BssNAI	GTATAC	1 cut(s)	1634
BssSI	CACGAG	1 cut(s)	857
BssT1I	CCWWGG	1 cut(s)	121
Bst1107I	GTATAC	1 cut(s)	1634
Bst2BI	CACGAG	1 cut(s)	857
Bst2UI	CCWGG	1 cut(s)	528
Bst4CI	ACNGT	8 cut(s)	208, 220, 296, 874, 940, 1127, 1179, 1531
Bst6I	CTCTTC	1 cut(s)	1116
BstAUI	TGTACA	1 cut(s)	1326
BstC8I	GCNNGC	3 cut(s)	949, 1432, 1555
BstDEI	CTNAG	4 cut(s)	42, 172, 560, 1130
BstDSI	CCRYGG	1 cut(s)	121
BstENI	CCTNNNNNAGG	1 cut(s)	171
BstF5I	GGATG	2 cut(s)	363, 1588
BstFNI	CGCG	3 cut(s)	76, 93, 633
BstHHI	GCGC	3 cut(s)	32, 78, 1278
BstMAI	GTCTC	5 cut(s)	60, 381, 655, 914, 975
BstMWI	GCNNNNNNNGC	4 cut(s)	82, 413, 731, 1339
BstNI	CCWGG	1 cut(s)	528
BstNSI	RCATGY	4 cut(s)	349, 1095, 1557, 1640
BstPAI	GACNNNNGTC	1 cut(s)	938
BstSCI	CCNGG	2 cut(s)	526, 1534
BstSFI	CTRYAG	1 cut(s)	1480
BstUI	CGCG	3 cut(s)	76, 93, 633
BstV1I	GCAGC	9 cut(s)	14, 712, 748, 830, 1504, 1569, 1609, 1630, 1652
BstV2I	GAAGAC	1 cut(s)	1676
BstZ17I	GTATAC	1 cut(s)	1634
Bsu15I	ATCGAT	1 cut(s)	274
Bsu36I	CCTNAGG	1 cut(s)	172
BsuI	GTATCC	1 cut(s)	1466
BsuRI	GGCC	3 cut(s)	47, 1136, 1243
BsuTUI	ATCGAT	1 cut(s)	274
BtgI	CCRYGG	1 cut(s)	121
BtgZI	GCGATG	1 cut(s)	74
BtrI	CACGTC	1 cut(s)	767
BtsCI	GGATG	2 cut(s)	363, 1588
BtsI	GCAGTG	1 cut(s)	295
BtsIMutI	CAGTG	3 cut(s)	295, 776, 936
Cac8I	GCNNGC	3 cut(s)	949, 1432, 1555
CaiI	CAGNNNCTG	2 cut(s)	294, 761
CfoI	GCGC	3 cut(s)	32, 78, 1278
Cfr13I	GGNCC	6 cut(s)	95, 163, 1003, 1134, 1541, 1593
ClaI	ATCGAT	1 cut(s)	274
Csp6I	GTAC	5 cut(s)	571, 677, 1120, 1175, 1327
CspCI	CAANNNNNGTGG	2 cut(s)	1554, 1589
CviAII	CATG	9 cut(s)	122, 326, 346, 400, 439, 1092, 1391, 1554, 1637
CviQI	GTAC	5 cut(s)	571, 677, 1120, 1175, 1327
DdeI	CTNAG	4 cut(s)	42, 172, 560, 1130
DraI	TTTAAA	1 cut(s)	813
DrdI	GACNNNNNNGTC	1 cut(s)	206
DseDI	GACNNNNNNGTC	1 cut(s)	206
Eam1104I	CTCTTC	1 cut(s)	1116
EarI	CTCTTC	1 cut(s)	1116
Eco130I	CCWWGG	1 cut(s)	121
Eco147I	AGGCCT	1 cut(s)	1243
Eco31I	GGTCTC	1 cut(s)	60
Eco47I	GGWCC	5 cut(s)	95, 163, 1003, 1541, 1593
Eco57I	CTGAAG	2 cut(s)	459, 1134
Eco81I	CCTNAGG	1 cut(s)	172
Eco88I	CYCGRG	2 cut(s)	408, 1170
EcoNI	CCTNNNNNAGG	1 cut(s)	171
EcoRI	GAATTC	2 cut(s)	9, 1193
EcoRII	CCWGG	1 cut(s)	526
EcoT14I	CCWWGG	1 cut(s)	121
ErhI	CCWWGG	1 cut(s)	121
FaeI	CATG	9 cut(s)	125, 329, 349, 403, 442, 1095, 1394, 1557, 1640
FalI	AAGNNNNNCTT	4 cut(s)	1256, 1288, 1307, 1339
FatI	CATG	9 cut(s)	121, 325, 345, 399, 438, 1091, 1390, 1553, 1636
FauI	CCCGC	1 cut(s)	101
FbaI	TGATCA	2 cut(s)	1236, 1294
FblI	GTMKAC	2 cut(s)	1440, 1633
FokI	GGATG	2 cut(s)	370, 1595
FspBI	CTAG	1 cut(s)	543
FspI	TGCGCA	1 cut(s)	1277
GlaI	GCGC	3 cut(s)	31, 77, 1277
HaeIII	GGCC	3 cut(s)	47, 1136, 1243
HapII	CCGG	2 cut(s)	699, 1536
HhaI	GCGC	3 cut(s)	32, 78, 1278
Hin1II	CATG	9 cut(s)	125, 329, 349, 403, 442, 1095, 1394, 1557, 1640
Hin6I	GCGC	3 cut(s)	30, 76, 1276
HinP1I	GCGC	3 cut(s)	30, 76, 1276
HincII	GTYRAC	1 cut(s)	343
HindII	GTYRAC	1 cut(s)	343
HindIII	AAGCTT	1 cut(s)	1432
HinfI	GANTC	6 cut(s)	276, 510, 710, 1412, 1655, 1691
HpaII	CCGG	2 cut(s)	699, 1536
HphI	GGTGA	4 cut(s)	239, 1025, 1073, 1106
Hpy166II	GTNNAC	4 cut(s)	163, 343, 1441, 1634
Hpy188I	TCNGA	7 cut(s)	281, 478, 757, 944, 1024, 1131, 1299
Hpy8I	GTNNAC	4 cut(s)	163, 343, 1441, 1634
HpyAV	CCTTC	7 cut(s)	259, 558, 640, 875, 1010, 1330, 1463
HpyCH4III	ACNGT	8 cut(s)	208, 220, 296, 874, 940, 1127, 1179, 1531
HpyCH4IV	ACGT	1 cut(s)	766
HpyCH4V	TGCA	7 cut(s)	115, 764, 893, 1557, 1625, 1640, 1717
HpyF10VI	GCNNNNNNNGC	4 cut(s)	82, 413, 731, 1339
HpyF3I	CTNAG	4 cut(s)	42, 172, 560, 1130
HpySE526I	ACGT	1 cut(s)	766
Hsp92II	CATG	9 cut(s)	125, 329, 349, 403, 442, 1095, 1394, 1557, 1640
HspAI	GCGC	3 cut(s)	30, 76, 1276
Kpn2I	TCCGGA	1 cut(s)	698
Ksp22I	TGATCA	2 cut(s)	1236, 1294
LguI	GCTCTTC	1 cut(s)	1116
LmnI	GCTCC	2 cut(s)	105, 1339
Lsp1109I	GCAGC	9 cut(s)	14, 712, 748, 830, 1504, 1569, 1609, 1630, 1652
LweI	GCATC	6 cut(s)	97, 403, 541, 748, 880, 1573
MaeI	CTAG	1 cut(s)	543
MaeII	ACGT	1 cut(s)	766
MbiI	CCGCTC	2 cut(s)	108, 187
MboII	GAAGA	6 cut(s)	344, 607, 911, 1103, 1111, 1681
MluCI	AATT	6 cut(s)	9, 335, 462, 782, 1193, 1257
MlyI	GAGTC	2 cut(s)	704, 1685
MmeI	TCCRAC	1 cut(s)	1481
MroI	TCCGGA	1 cut(s)	698
MseI	TTAA	3 cut(s)	222, 656, 812
MslI	CAYNNNNRTG	4 cut(s)	120, 398, 769, 956
MspA1I	CMGCKG	2 cut(s)	1368, 1643
MspI	CCGG	2 cut(s)	699, 1536
MspR9I	CCNGG	2 cut(s)	528, 1536
Mva1269I	GAATGC	1 cut(s)	1519
MvaI	CCWGG	1 cut(s)	528
MvnI	CGCG	3 cut(s)	76, 93, 633
MwoI	GCNNNNNNNGC	4 cut(s)	82, 413, 731, 1339
NciI	CCSGG	1 cut(s)	1536
NcoI	CCATGG	1 cut(s)	121
NlaIII	CATG	9 cut(s)	125, 329, 349, 403, 442, 1095, 1394, 1557, 1640
NlaIV	GGNNCC	2 cut(s)	165, 1062
NmuCI	GTSAC	7 cut(s)	214, 290, 714, 737, 777, 931, 1688
NsbI	TGCGCA	1 cut(s)	1277
NspI	RCATGY	4 cut(s)	349, 1095, 1557, 1640
OliI	CACNNNNGTG	1 cut(s)	769
PaeI	GCATGC	1 cut(s)	1557
PaeR7I	CTCGAG	2 cut(s)	408, 1170
PceI	AGGCCT	1 cut(s)	1243
PciSI	GCTCTTC	1 cut(s)	1116
PctI	GAATGC	1 cut(s)	1519
PfeI	GAWTC	4 cut(s)	276, 510, 1412, 1655
PleI	GAGTC	2 cut(s)	704, 1685
PpsI	GAGTC	2 cut(s)	704, 1685
PshAI	GACNNNNGTC	1 cut(s)	938
Psp6I	CCWGG	1 cut(s)	526
PspGI	CCWGG	1 cut(s)	526
PspN4I	GGNNCC	2 cut(s)	165, 1062
PspPI	GGNCC	6 cut(s)	95, 163, 1003, 1134, 1541, 1593
PspXI	VCTCGAGB	1 cut(s)	1170
PstNI	CAGNNNCTG	2 cut(s)	294, 761
PvuII	CAGCTG	2 cut(s)	1368, 1643
RsaI	GTAC	5 cut(s)	572, 678, 1121, 1176, 1328
RsaNI	GTAC	5 cut(s)	571, 677, 1120, 1175, 1327
RseI	CAYNNNNRTG	4 cut(s)	120, 398, 769, 956
SapI	GCTCTTC	1 cut(s)	1116
SaqAI	TTAA	3 cut(s)	222, 656, 812
Sau96I	GGNCC	6 cut(s)	95, 163, 1003, 1134, 1541, 1593
SchI	GAGTC	2 cut(s)	704, 1685
ScrFI	CCNGG	2 cut(s)	528, 1536
SfaNI	GCATC	6 cut(s)	97, 403, 541, 748, 880, 1573
SfcI	CTRYAG	1 cut(s)	1480
Sfr274I	CTCGAG	2 cut(s)	408, 1170
SinI	GGWCC	5 cut(s)	95, 163, 1003, 1541, 1593
SlaI	CTCGAG	2 cut(s)	408, 1170
SmiMI	CAYNNNNRTG	4 cut(s)	120, 398, 769, 956
SmlI	CTYRAG	2 cut(s)	408, 1170
SmoI	CTYRAG	2 cut(s)	408, 1170
SphI	GCATGC	1 cut(s)	1557
Sse9I	AATT	6 cut(s)	9, 335, 462, 782, 1193, 1257
SseBI	AGGCCT	1 cut(s)	1243
SsiI	CCGC	7 cut(s)	52, 85, 93, 108, 185, 404, 1586
SspI	AATATT	2 cut(s)	809, 1502
SspMI	CTAG	1 cut(s)	543
StuI	AGGCCT	1 cut(s)	1243
StyD4I	CCNGG	2 cut(s)	526, 1534
StyI	CCWWGG	1 cut(s)	121
TaaI	ACNGT	8 cut(s)	208, 220, 296, 874, 940, 1127, 1179, 1531
TaiI	ACGT	1 cut(s)	769
TaqI	TCGA	7 cut(s)	66, 274, 409, 797, 1145, 1171, 1526
TasI	AATT	6 cut(s)	9, 335, 462, 782, 1193, 1257
TatI	WGTACW	1 cut(s)	1326
TauI	GCSGC	3 cut(s)	88, 187, 407
TfiI	GAWTC	4 cut(s)	276, 510, 1412, 1655
Tru1I	TTAA	3 cut(s)	222, 656, 812
Tru9I	TTAA	3 cut(s)	222, 656, 812
TscAI	CASTG	3 cut(s)	295, 776, 943
TseFI	GTSAC	7 cut(s)	214, 290, 714, 737, 777, 931, 1688
Tsp45I	GTSAC	7 cut(s)	214, 290, 714, 737, 777, 931, 1688
TspDTI	ATGAA	7 cut(s)	388, 497, 502, 1041, 1118, 1186, 1439
TspRI	CASTG	3 cut(s)	295, 776, 943
VpaK11BI	GGWCC	5 cut(s)	95, 163, 1003, 1541, 1593
XagI	CCTNNNNNAGG	1 cut(s)	171
XapI	RAATTY	3 cut(s)	9, 335, 1193
XceI	RCATGY	4 cut(s)	349, 1095, 1557, 1640
XhoI	CTCGAG	2 cut(s)	408, 1170
XmiI	GTMKAC	2 cut(s)	1440, 1633
XspI	CTAG	1 cut(s)	543

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.