FvH4_2g29840
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000493
Physical Location & Seq
Forward (+)
23095437 .. 23103432
7996 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g29840.t1

Sequence Viewer

Length: 4269 bp
ATGGAAGTGTTTTCAAGGTCTTTTCGAGGTGAAGACGATGAAGAAGCTCTCAAATGGGCTGCAATTGAGAGGCTGCCTACAGGTTTGCGTATCGGTAGAGGCTTGTTCATTGATGGGGAGGGCCAAGCTAGAGAGGTTGATGTCGAAAACCTTGGTTTACTTGAGAGGAAGACTTTGTTGGAGAGGCTGGTTAACAATGCTGAGAAAGATAATGAGAAGTTCTTGTTAAAGCTCAAGAACCGAATTGACAGAGTTGGACTTGATATGCCAACAATTGAAGTTCGGTTTGAGCATCTAACTGTGGAGGCACAAGTTTATTTGGGAAGCAGAGCACTGCCTACTTTACTCAACTTTGCCATCAATATGATACAGGGATGGTTACATTATCTTCACATTTTTCCAAGTAGAAAGGCATCCTTAACAATCCTTCAAAATGTTAGTGGCATTATCAAGCCCCAAAGAATGACCTTGCTTTTAGGCCCCCCAAGTTCCGGAAAAACCACATTACTACTGGCTCTTGCTGGAAGACTCGGTAAAGATTTAAAATCTTCAGGAGACGTTACGTATAATGGGCACAGTATGGCAGAATTTGTACCTCAGAGAACATCAGCTTATATATGTCAGAAAGATCTTCACATAGGAGAAATGACAGTTAAGGAGACACTGGCTTTCTCAGCTAGATGTCAAGGAGTTGGGTCAAATTGCGAAATGTTGGCAGAACTGTCTAGAAGAGAGAAGGATGAAAATATTAAGCCTGATCCAGACATTGATATCTACCAGAAGGCAGCAGCACTAGAAGGGCAGGAGAGTAGTGTAGTTGCTGATTATATTCTCAAGATTTTGGGACTTGAGGTTTGTGCCAATACTATGGTGGGGGATGAAATGGTAAGAGGCATCTCGGGTGGAGAAAGAAAGCGAGTCACAATAGGGGAGATGCTTGTTGGACCAGTAAGAGCTCTCTTCATGGATGAGATATCAACTGGTTTGGACAGTTCAACAACTTTCCAGATAGTAAACTCACTCAGGCAATCTATCCATATTCTTAGGGGAACTGGAGTTGTTTCCCTCCTACAGCCAGCCCCCGAAACTTATAACCTATTCGATGATATAATTCTACTATCAGATGGCCAAATAGTGTATCAGGGTCCACGCGAATGTGTGCTCGGCTTTTTTGAATTCATGGGGTTCAAATGTCCTGAGAGGAAAGGGGTTGCTGATTTCTTGCAAGAGGTGACATCCAGGAAAGACCAAGAGCAGTATTGGGCACGTTTACATGAGCCTTACAGATATGTTACTGTCAATGAGTTTGCTGAAGCATTCCAGGCATTTCATATTGGCCAGAAACTTGGTGATGAGCTTGCTAGTCCCTTCGACAGGTCCAAAAGCCACCGTGCTGCTTTAACAGCTAAGAAATACGGTGTTAGCAAGACGGAGTTGTTCAGAGCTTGTGTTTCTAGAGAGTTTTTGCTCATGAAGAGGAACTCATTTGCCTACATTTTCAAATTGGCCCTACTTATCATCTTAGCTTCTATAACAACAACAATATTTATGAGAGTTGAGATGCATAAAGGCTCTGTGTTGGATGGGGGAATCTATCTTGGTGCTTTGTTCTTTGCGGTCATTGTAGCTATGTTTAATGGAATATCAGAGCTGAACATGGCCATTATGAAACTTCCCATCTTTTACAAGCAAAGAGACCTACTCTTCTATCCTGCATGGGCATACTCAATACCTGGATGGATACTCAAGATCCCAATCACTTTAGTAGAGAGTTTCATTTGGGTGATCATTACTTATTATGTTATAGGATTTGATCCACATATTGAAAGGTTCCTCAAGCAGTACATGATACTGGTGTGCATAAACCAGATGGCGTCCGGGTTGTTCAGAGCCATGGCTGCAATAGGAAGGGATGTAATAGTTGCTACCACATTTGGAACCTTTGCATTACTTATAATCTTGGTTTTGGGTGGATTTGTCTTATCTCGAGAGGCTATACCAAAATGGTGGTTGTGGGGGTACTGGATCTCACCGTTGATGTATGGACAAAATGCCATAGCCGTGAATGAATTTCTCGGAAAGAGTTGGAGCCATGAGCCACCTGGTTCTACAGAATCCTTGGGAGTAATGGTCCTGAAGTCTCATGGCATTTTCCCAGAAGCACATTGGTATTGGATTGGAGTAGCAGCTTTAATTGGATACACTTTTCTGTTCAACATCATCATTACTTTCGCTCTACAATATCTTGATCCATTTGGGAAACCTCAGGTAGTACTGTCCAAGGAAAGCATCGCGGAGAGAAATGGTAACGGAACTGGAGAGTTCATTGAGCTGTTATCAAGAGGAAATAAGTCTTCTGAAAGTATTGTTGAAGGTGCAACAAGCTTGTCATCATCTACAAGTGGAGGAAGAAGTGATAATGCTAATGGGAGAAGAAAGCGAGGAATGGTTCTTCCTTTTCAACCCCTTTCCCTTGCTTTCGATGAGATCAGATATGCCATAGACATGCCACAGGAAATGAAGGCTCAGGGCGTCAAGGAGGATAGGCTGGAACTGTTAAAAGGTGTAAGTGGTGCTTTTAGACCAGGAGTCTTAACTGCCCTCATGGGTGTTAGTGGTGCAGGCAAGACCACTCTAATGGATGTCTTGGCCGGAAGGAAAACTGGTGGACACATTGATGGAAGCATCATGATATCTGGGTACAGTAAAAGGCAAGAGACATTTGCTCGCATATCAGGTTATTGTGAGCAAACTGATATCCACTCTCCTCATGTTACAGTCTATGAATCCCTCCTGTTTTCTGCTTGGCTTCGACTACTCCCAGAGGTTGATCCTGCTACTAGAAAGATGTTTATTGAGGAAGTCATGGAGCTTGTGGAGCTGACTTCATTAAGGGGAGCACTTGTGGGACTACCAGGTGTAACTGGTCTTTCAACCGAGCAGCGCAAAAGACTGACAATTGCCGTTGAGCTTGTTGCAAATCCATCTATAATTTTCATGGATGAGCCAACATCTGGCCTTGATGCCAGGGCAGCAGCAATAGTGATGAGAACAGTTAGGAACACAGTGGACACAGGAAGAACTGTGGTTTGCACAATCCACCAGCCTAGCATAGATATATTTGAATCTTTTGATGAGCTTCTTCTTCTCAAATTAGGAGGTGAGCCAATATATGCTGGTCCATTAGGCCTCCATTCCTCCCATCTGATAGAGTACTTTGAGGGAATTAATGGTGTTCCTAAAATTAAAGATGGGTATAATCCTGCAACCTGGATGTTGGAGATTACTTCAGCAGCACAAGAAGAGGCTCTTGGGGTCAACTTTACAGATGTGTACAAGAAATCAGAACTATACAAGAGAATGAAAGACCTGATCAAAGGACTGTCTATACCTCCTCCAGGTTCTAAAGACCTGTACTTCCCTACTCGCTATTCACAATCTTTTCACACGCAATGCATGGCCTGCCTATGGAAACAACACTGGTCCTACTGGCGAAACCCATCATATAGTGCAGTTAGACTCTTATTCACAACATTCATCGCATTTTTGTTTGGTATGATATTTTGGGATCTTGGATCAAAAAGAAGCAAGCAGCAAGATATCTTCAATGCAATGGGATCTATGTATTCTGCTGTAATCTTTCTCGGGGTCCAGAATTCTGCCTCAGTGCAGCCAATTGTGGATGTTGAGAGGACAGTATTTTACAGAGAAAGAGCAGCTGGTATGTACTCGGCTTTGCCTTACGCTTTTGGACAGATGATGATTGAAGTTCCCTACATTTTCATCCAAACAATTATCTATGGGGTTATAGTATACACAATGATGGGATTCGATTGGACAGTTTCCAAGTTCCTTTGGTACCTTTTCTTCATGTACTTCACCTTATTGTACTTCACATTCTATGGCATGATGACCATGGCACTCACTCCCAACCACAACATTGCTGCTATAACTTCATCTTCCTTCTATGCAATATGGAACCTTTTCTCAGGATTTGTCATTCCTCCATCGAGAATACCTATATGGTGGAAATGGTACTATTGGATTTGCCCTGTTGCTTGGACCTTGTATGGAATGGCCGCTTCGCAGTTTGGAGACATAGAGCACAAACTTGATACTGGCGAAGCTGTTGAAGATTTTCTGACAAGTTACTTTGGATACAGGCACGATTTTCTGGCCGTCGTTTCAGTGGTGGTTGTGGGGTTTTCAGTATTGTTCGGCTTTATCTTTGCTTATTCCATTAGAGCATTTAACTTCCAAAAAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1423

Amino Acids

159.92

Weight (kDa)

6.83

Isoelectric Point (pI)

38.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 66 - 118 4.7e-08 ABC-transporter N-terminal
ABC_tran PF00005 143 - 324 3.4e-15 ABC transporter
ABC2_membrane PF01061 479 - 691 2.3e-48 ABC-2 type transporter
PDR_assoc PF08370 696 - 758 1.9e-27 Plant PDR ABC transporter associated
ABC_tran PF00005 853 - 1005 4.9e-19 ABC transporter
ABC2_membrane PF01061 1150 - 1364 9.7e-60 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000493)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29820 FvH4_2g29820 FvH4_2g29822 FvH4_2g29824 FvH4_2g29824 FvH4_2g29824 FvH4_2g29840 FvH4_2g29840 FvH4_2g29840 FvH4_5g00550 FvH4_5g00550
malus_domestica MD02G1032700.v1.1 MD08G1140900.v1.1 MD08G1141400.v1.1 MD15G1117800.v1.1 MD15G1117900.v1.1
prunus_persica Prupe.1G471200_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471400_v2.0.a1 Prupe.7G242900_v2.0.a1
pyrus_communis pycom02g02690 pycom08g11890
rosa_chinensis RchiOBHm_Chr2g0111741 RchiOBHm_Chr2g0145991 RchiOBHm_Chr6g0298851 RchiOBHm_Chr6g0298871 RchiOBHm_Chr6g0298891 RchiOBHm_Chr6g0298901 RchiOBHm_Chr6g0298911 RchiOBHm_Chr7g0201801 RchiOBHm_Chr7g0211531 RchiOBHm_Chr7g0214601
rosa_laevigata RLG00000002931 RLG00000003000 RLG00000003647 RLG00000011476 RLG00000011479
rosa_multiflora Rmu_sc0001030.1_g000012 Rmu_sc0001548.1_g000041 Rmu_ssc0000397.1_g000033 Rmu_ssc0000397.1_g000036 Rmu_ssc0000397.1_g000037 Rmu_ssc0000397.1_g000039 Rmu_ssc0000397.1_g000048
rosa_roxburghii Rroxscaffold_1G00003830 Rroxscaffold_3G00247810 Rroxscaffold_3G00255060 Rroxscaffold_7G00169220 Rroxscaffold_7G00169230 Rroxscaffold_7G00169280
rosa_rugosa Rorug02G0223600 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283200 Rorug06G0283300 Rorug06G0283400 Rorug06G0283500 Rorug07G0066000
rosa_samantha Rh2AG511300 Rh2CG235100 Rh2CG496400 Rh2DG330900 Rh6BG403100 Rh6BG403300 Rh6BG403400 Rh6BG403500 Rh6CG408700 Rh6CG408900 Rh6CG409000 Rh6CG409200 Rh6DG395800 Rh6DG395900 Rh6DG396000 Rh6DG396200 Rh7AG193400 Rh7BG195100 Rh7BG436600 Rh7DG199300 Rh7DG265500
rosa_wichuraiana Rw2G016900 Rw6G034570 Rw6G034580 Rw6G034590 Rw6G034600 Rw7G016900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1092, 1955
Acc65I GGTACC 1 cut(s) 3858
AccB1I GGYRCC 1 cut(s) 3858
AccB7I CCANNNNNTGG 1 cut(s) 3014
AccI GTMKAC 1 cut(s) 3813
AccII CGCG 2 cut(s) 1152, 2294
AccIII TCCGGA 1 cut(s) 491
AciI CCGC 3 cut(s) 1616, 2294, 4080
AclWI GGATC 9 cut(s) 752, 1744, 1808, 2033, 2243, 2825, 3576, 3583, 3625
AcoI YGGCCR 6 cut(s) 1126, 1336, 1659, 2649, 4077, 4176
AcsI RAATTY 4 cut(s) 587, 1175, 2069, 3655
AcuI CTGAAG 4 cut(s) 534, 1332, 2156, 3273
AcyI GRCGYC 2 cut(s) 1874, 2532
AfiI CCNNNNNNNGG 5 cut(s) 491, 1374, 3014, 3398, 3468
AhdI GACNNNNNGTC 1 cut(s) 2588
AjnI CCWGG 9 cut(s) 1238, 1320, 1732, 2101, 2584, 2914, 3026, 3269, 3397
AjuI GAANNNNNNNTTGG 4 cut(s) 161, 193, 3294, 3326
Alw21I GWGCWC 5 cut(s) 334, 958, 1164, 2902, 4107
Alw26I GTCTC 6 cut(s) 549, 653, 1689, 2145, 2711, 4089
AlwI GGATC 9 cut(s) 752, 1744, 1808, 2033, 2243, 2825, 3576, 3583, 3625
Ama87I CYCGRG 3 cut(s) 898, 1986, 3644
Aor13HI TCCGGA 1 cut(s) 491
ApoI RAATTY 4 cut(s) 587, 1175, 2069, 3655
ArsI GACNNNNNNTTYG 2 cut(s) 837, 869
AseI ATTAAT 1 cut(s) 3228
Asp700I GAANNNNTTC 2 cut(s) 3983, 4137
Asp718I GGTACC 1 cut(s) 3858
AspLEI GCGC 1 cut(s) 2946
AsuC2I CCSGG 1 cut(s) 1879
AsuHPI GGTGA 7 cut(s) 41, 1243, 1361, 1795, 2022, 3173, 3871
AvaI CYCGRG 3 cut(s) 898, 1986, 3644
AvaII GGWCC 8 cut(s) 944, 1145, 1377, 2131, 3179, 3483, 3649, 4062
AxyI CCTNAGG 1 cut(s) 2265
BaeGI GKGCMC 2 cut(s) 576, 1267
BaeI ACNNNNGTAYC 2 cut(s) 1841, 1874
BalI TGGCCA 3 cut(s) 1128, 1338, 1661
BanI GGYRCC 1 cut(s) 3858
BanII GRGCYC 1 cut(s) 958
BarI GAAGNNNNNNTAC 2 cut(s) 3851, 3883
BbsI GAAGAC 4 cut(s) 39, 176, 532, 2346
Bbv12I GWGCWC 5 cut(s) 334, 958, 1164, 2902, 4107
BceAI ACGGC 3 cut(s) 2045, 2948, 4163
BciT130I CCWGG 9 cut(s) 1240, 1322, 1734, 2103, 2586, 2916, 3028, 3271, 3399
BciVI GTATCC 3 cut(s) 1734, 2192, 4151
BclI TGATCA 2 cut(s) 1785, 3372
BcnI CCSGG 1 cut(s) 1879
BcoDI GTCTC 6 cut(s) 549, 653, 1689, 2145, 2711, 4089
BfaI CTAG 8 cut(s) 129, 678, 726, 794, 1362, 1455, 2841, 3108
BfmI CTRYAG 3 cut(s) 78, 1070, 2109
BfuI GTATCC 3 cut(s) 1734, 2192, 4151
BglII AGATCT 1 cut(s) 628
BmcAI AGTACT 2 cut(s) 2274, 3215
Bme18I GGWCC 8 cut(s) 944, 1145, 1377, 2131, 3179, 3483, 3649, 4062
BmeRI GACNNNNNGTC 1 cut(s) 2588
BmeT110I CYCGRG 3 cut(s) 898, 1986, 3644
BmiI GGNNCC 8 cut(s) 481, 1146, 1832, 1939, 2090, 3650, 3860, 3980
BmsI GCATC 8 cut(s) 301, 422, 903, 924, 1551, 2298, 2694, 3013
BpiI GAAGAC 4 cut(s) 39, 176, 532, 2346
BplI GAGNNNNNCTC 2 cut(s) 1686, 1718
BpmI CTGGAG 3 cut(s) 1074, 2337, 3381
Bpu10I CCTNAGC 1 cut(s) 2526
BpuEI CTTGAG 6 cut(s) 182, 218, 818, 869, 1730, 1820
BpuMI CCSGG 1 cut(s) 1879
BsaAI YACGTR 1 cut(s) 564
BsaBI GATNNNNATC 1 cut(s) 1754
BsaHI GRCGYC 2 cut(s) 1874, 2532
BsaI GGTCTC 1 cut(s) 1689
BsaJI CCNNGG 6 cut(s) 151, 1893, 2118, 2280, 3027, 3915
BsaWI WCCGGW 1 cut(s) 491
BsaXI ACNNNNNCTCC 4 cut(s) 2115, 2145, 2889, 2919
Bsc4I CCNNNNNNNGG 5 cut(s) 491, 1374, 3014, 3398, 3468
Bse21I CCTNAGG 1 cut(s) 2265
Bse3DI GCAATG 3 cut(s) 3458, 3618, 3939
Bse8I GATNNNNATC 1 cut(s) 1754
BseAI TCCGGA 1 cut(s) 491
BseBI CCWGG 9 cut(s) 1240, 1322, 1734, 2103, 2586, 2916, 3028, 3271, 3399
BseDI CCNNGG 6 cut(s) 151, 1893, 2118, 2280, 3027, 3915
BseJI GATNNNNATC 1 cut(s) 1754
BseLI CCNNNNNNNGG 5 cut(s) 491, 1374, 3014, 3398, 3468
BseMI GCAATG 3 cut(s) 3458, 3618, 3939
BseMII CTCAG 9 cut(s) 192, 611, 687, 1036, 1188, 2279, 2540, 3678, 4002
BseRI GAGGAG 2 cut(s) 2757, 3384
BseSI GKGCMC 2 cut(s) 576, 1267
BsgI GTGCAG 3 cut(s) 2640, 3531, 3689
Bsh1236I CGCG 2 cut(s) 1152, 2294
BshNI GGYRCC 1 cut(s) 3858
BsiHKAI GWGCWC 5 cut(s) 334, 958, 1164, 2902, 4107
BsiHKCI CYCGRG 3 cut(s) 898, 1986, 3644
BsiSI CCGG 3 cut(s) 492, 1878, 2652
BslFI GGGAC 3 cut(s) 858, 1350, 2922
BslI CCNNNNNNNGG 5 cut(s) 491, 1374, 3014, 3398, 3468
BsmAI GTCTC 6 cut(s) 549, 653, 1689, 2145, 2711, 4089
BsmBI CGTCTC 1 cut(s) 549
BsmFI GGGAC 3 cut(s) 858, 1350, 2922
BsmI GAATGC 1 cut(s) 1316
Bso31I GGTCTC 1 cut(s) 1689
BsoBI CYCGRG 3 cut(s) 898, 1986, 3644
Bsp1286I GDGCHC 7 cut(s) 334, 576, 958, 1164, 1267, 2902, 4107
Bsp13I TCCGGA 1 cut(s) 491
Bsp1407I TGTACA 1 cut(s) 3333
Bsp19I CCATGG 2 cut(s) 1893, 3915
BspACI CCGC 3 cut(s) 1616, 2294, 4080
BspCNI CTCAG 9 cut(s) 193, 610, 686, 1035, 1189, 2278, 2539, 3677, 4001
BspEI TCCGGA 1 cut(s) 491
BspFNI CGCG 2 cut(s) 1152, 2294
BspHI TCATGA 2 cut(s) 1470, 2688
BspLI GGNNCC 8 cut(s) 481, 1146, 1832, 1939, 2090, 3650, 3860, 3980
BspPI GGATC 9 cut(s) 752, 1744, 1808, 2033, 2243, 2825, 3576, 3583, 3625
BspT107I GGYRCC 1 cut(s) 3858
BspTNI GGTCTC 1 cut(s) 1689
BsrDI GCAATG 3 cut(s) 3458, 3618, 3939
BsrGI TGTACA 1 cut(s) 3333
BssECI CCNNGG 6 cut(s) 151, 1893, 2118, 2280, 3027, 3915
BssNAI GTATAC 1 cut(s) 3814
BssNI GRCGYC 2 cut(s) 1874, 2532
BssT1I CCWWGG 5 cut(s) 151, 1893, 2118, 2280, 3915
Bst1107I GTATAC 1 cut(s) 3814
Bst2UI CCWGG 9 cut(s) 1240, 1322, 1734, 2103, 2586, 2916, 3028, 3271, 3399
Bst6I CTCTTC 5 cut(s) 724, 965, 1469, 1709, 3297
BstACI GRCGYC 2 cut(s) 1874, 2532
BstAPI GCANNNNNTGC 1 cut(s) 3462
BstAUI TGTACA 1 cut(s) 3333
BstBAI YACGTR 1 cut(s) 564
BstC8I GCNNGC 6 cut(s) 1077, 1359, 2623, 2728, 3463, 3590
BstDSI CCRYGG 2 cut(s) 1893, 3915
BstENI CCTNNNNNAGG 2 cut(s) 1372, 3396
BstFNI CGCG 2 cut(s) 1152, 2294
BstHHI GCGC 1 cut(s) 2946
BstMAI GTCTC 6 cut(s) 549, 653, 1689, 2145, 2711, 4089
BstMWI GCNNNNNNNGC 7 cut(s) 674, 1322, 1403, 1898, 2878, 3032, 3462
BstNI CCWGG 9 cut(s) 1240, 1322, 1734, 2103, 2586, 2916, 3028, 3271, 3399
BstNSI RCATGY 1 cut(s) 2509
BstSFI CTRYAG 3 cut(s) 78, 1070, 2109
BstSLI GKGCMC 2 cut(s) 576, 1267
BstSNI TACGTA 1 cut(s) 564
BstUI CGCG 2 cut(s) 1152, 2294
BstV2I GAAGAC 4 cut(s) 39, 176, 532, 2346
BstX2I RGATCY 5 cut(s) 628, 1749, 2025, 3568, 3617
BstXI CCANNNNNNTGG 4 cut(s) 868, 1346, 2007, 2638
BstYI RGATCY 5 cut(s) 628, 1749, 2025, 3568, 3617
BstZ17I GTATAC 1 cut(s) 3814
Bsu36I CCTNAGG 1 cut(s) 2265
BsuI GTATCC 3 cut(s) 1734, 2192, 4151
BtgI CCRYGG 2 cut(s) 1893, 3915
BtgZI GCGATG 2 cut(s) 2275, 3523
BtsI GCAGTG 1 cut(s) 332
BtsIMutI CAGTG 6 cut(s) 332, 662, 3072, 3478, 3672, 4194
Cac8I GCNNGC 6 cut(s) 1077, 1359, 2623, 2728, 3463, 3590
CciI TCATGA 2 cut(s) 1470, 2688
CfoI GCGC 1 cut(s) 2946
CseI GACGC 2 cut(s) 1863, 2521
CsiI ACCWGGT 2 cut(s) 2101, 2914
DraI TTTAAA 1 cut(s) 543
DriI GACNNNNNGTC 1 cut(s) 2588
EaeI YGGCCR 6 cut(s) 1126, 1336, 1659, 2649, 4077, 4176
Eam1104I CTCTTC 5 cut(s) 724, 965, 1469, 1709, 3297
Eam1105I GACNNNNNGTC 1 cut(s) 2588
EarI CTCTTC 5 cut(s) 724, 965, 1469, 1709, 3297
Ecl136II GAGCTC 1 cut(s) 956
Eco105I TACGTA 1 cut(s) 564
Eco130I CCWWGG 5 cut(s) 151, 1893, 2118, 2280, 3915
Eco147I AGGCCT 1 cut(s) 3189
Eco24I GRGCYC 1 cut(s) 958
Eco31I GGTCTC 1 cut(s) 1689
Eco32I GATATC 5 cut(s) 772, 975, 2694, 2758, 3601
Eco47I GGWCC 8 cut(s) 944, 1145, 1377, 2131, 3179, 3483, 3649, 4062
Eco53kI GAGCTC 1 cut(s) 956
Eco57I CTGAAG 4 cut(s) 534, 1332, 2156, 3273
Eco81I CCTNAGG 1 cut(s) 2265
Eco88I CYCGRG 3 cut(s) 898, 1986, 3644
EcoICRI GAGCTC 1 cut(s) 956
EcoNI CCTNNNNNAGG 2 cut(s) 1372, 3396
EcoO109I RGGNCCY 1 cut(s) 479
EcoRI GAATTC 2 cut(s) 1175, 3655
EcoRII CCWGG 9 cut(s) 1238, 1320, 1732, 2101, 2584, 2914, 3026, 3269, 3397
EcoRV GATATC 5 cut(s) 772, 975, 2694, 2758, 3601
EcoT14I CCWWGG 5 cut(s) 151, 1893, 2118, 2280, 3915
EcoT22I ATGCAT 2 cut(s) 1566, 3458
EcoT38I GRGCYC 1 cut(s) 958
ErhI CCWWGG 5 cut(s) 151, 1893, 2118, 2280, 3915
Esp3I CGTCTC 1 cut(s) 549
FalI AAGNNNNNCTT 6 cut(s) 401, 433, 2560, 2592, 3294, 3326
FaqI GGGAC 3 cut(s) 858, 1350, 2922
FbaI TGATCA 2 cut(s) 1785, 3372
FblI GTMKAC 1 cut(s) 3813
FriOI GRGCYC 1 cut(s) 958
FspBI CTAG 8 cut(s) 129, 678, 726, 794, 1362, 1455, 2841, 3108
GlaI GCGC 1 cut(s) 2945
GsuI CTGGAG 3 cut(s) 1074, 2337, 3381
HapII CCGG 3 cut(s) 492, 1878, 2652
HgaI GACGC 2 cut(s) 1863, 2521
HhaI GCGC 1 cut(s) 2946
Hin1I GRCGYC 2 cut(s) 1874, 2532
Hin6I GCGC 1 cut(s) 2944
HinP1I GCGC 1 cut(s) 2944
HincII GTYRAC 2 cut(s) 193, 3319
HindII GTYRAC 2 cut(s) 193, 3319
HindIII AAGCTT 1 cut(s) 2383
HinfI GANTC 9 cut(s) 528, 918, 1590, 2114, 2589, 2786, 3125, 3519, 3828
HpaI GTTAAC 1 cut(s) 193
HpaII CCGG 3 cut(s) 492, 1878, 2652
HphI GGTGA 7 cut(s) 41, 1243, 1361, 1795, 2022, 3173, 3871
Hpy99I CGWCG 1 cut(s) 4184
HpyCH4IV ACGT 3 cut(s) 558, 563, 1267
HpyF10VI GCNNNNNNNGC 7 cut(s) 674, 1322, 1403, 1898, 2878, 3032, 3462
HpySE526I ACGT 3 cut(s) 558, 563, 1267
Hsp92I GRCGYC 2 cut(s) 1874, 2532
HspAI GCGC 1 cut(s) 2944
Kpn2I TCCGGA 1 cut(s) 491
KpnI GGTACC 1 cut(s) 3862
Ksp22I TGATCA 2 cut(s) 1785, 3372
KspAI GTTAAC 1 cut(s) 193
LmnI GCTCC 4 cut(s) 2088, 2869, 2878, 2897
LweI GCATC 8 cut(s) 301, 422, 903, 924, 1551, 2298, 2694, 3013
MabI ACCWGGT 2 cut(s) 2101, 2914
MaeI CTAG 8 cut(s) 129, 678, 726, 794, 1362, 1455, 2841, 3108
MaeII ACGT 3 cut(s) 558, 563, 1267
MaeIII GTNAC 9 cut(s) 378, 559, 919, 1231, 1291, 2306, 2773, 2920, 4148
MfeI CAATTG 4 cut(s) 63, 273, 2958, 3675
MflI RGATCY 5 cut(s) 628, 1749, 2025, 3568, 3617
MhlI GDGCHC 7 cut(s) 334, 576, 958, 1164, 1267, 2902, 4107
MlsI TGGCCA 3 cut(s) 1128, 1338, 1661
MluNI TGGCCA 3 cut(s) 1128, 1338, 1661
MlyI GAGTC 4 cut(s) 522, 927, 2598, 3513
MmeI TCCRAC 6 cut(s) 159, 235, 922, 1560, 2066, 3258
Mox20I TGGCCA 3 cut(s) 1128, 1338, 1661
Mph1103I ATGCAT 2 cut(s) 1566, 3458
MroI TCCGGA 1 cut(s) 491
MroXI GAANNNNTTC 2 cut(s) 3983, 4137
MscI TGGCCA 3 cut(s) 1128, 1338, 1661
MslI CAYNNNNRTG 8 cut(s) 362, 1153, 1781, 2060, 2504, 2636, 2676, 3821
Msp20I TGGCCA 3 cut(s) 1128, 1338, 1661
MspA1I CMGCKG 1 cut(s) 3719
MspI CCGG 3 cut(s) 492, 1878, 2652
MunI CAATTG 4 cut(s) 63, 273, 2958, 3675
Mva1269I GAATGC 1 cut(s) 1316
MvaI CCWGG 9 cut(s) 1240, 1322, 1734, 2103, 2586, 2916, 3028, 3271, 3399
MvnI CGCG 2 cut(s) 1152, 2294
MwoI GCNNNNNNNGC 7 cut(s) 674, 1322, 1403, 1898, 2878, 3032, 3462
NciI CCSGG 1 cut(s) 1879
NcoI CCATGG 2 cut(s) 1893, 3915
NlaIV GGNNCC 8 cut(s) 481, 1146, 1832, 1939, 2090, 3650, 3860, 3980
NmeAIII GCCGAG 2 cut(s) 1143, 3710
NmuCI GTSAC 2 cut(s) 919, 1231
NsiI ATGCAT 2 cut(s) 1566, 3458
NspI RCATGY 1 cut(s) 2509
PaeR7I CTCGAG 1 cut(s) 1986
PagI TCATGA 2 cut(s) 1470, 2688
PceI AGGCCT 1 cut(s) 3189
PctI GAATGC 1 cut(s) 1316
PdmI GAANNNNTTC 2 cut(s) 3983, 4137
PfeI GAWTC 5 cut(s) 1590, 2114, 2786, 3125, 3828
PflMI CCANNNNNTGG 1 cut(s) 3014
PfoI TCCNGGA 1 cut(s) 1238
PleI GAGTC 4 cut(s) 522, 926, 2597, 3513
PpsI GAGTC 4 cut(s) 522, 926, 2597, 3513
Ppu21I YACGTR 1 cut(s) 564
PshBI ATTAAT 1 cut(s) 3228
PsiI TTATAA 2 cut(s) 1092, 1955
Psp124BI GAGCTC 1 cut(s) 958
Psp6I CCWGG 9 cut(s) 1238, 1320, 1732, 2101, 2584, 2914, 3026, 3269, 3397
PspGI CCWGG 9 cut(s) 1238, 1320, 1732, 2101, 2584, 2914, 3026, 3269, 3397
PspN4I GGNNCC 8 cut(s) 481, 1146, 1832, 1939, 2090, 3650, 3860, 3980
PsuI RGATCY 5 cut(s) 628, 1749, 2025, 3568, 3617
PvuII CAGCTG 1 cut(s) 3719
RseI CAYNNNNRTG 8 cut(s) 362, 1153, 1781, 2060, 2504, 2636, 2676, 3821
SacI GAGCTC 1 cut(s) 958
ScaI AGTACT 2 cut(s) 2274, 3215
SchI GAGTC 4 cut(s) 522, 927, 2598, 3513
SduI GDGCHC 7 cut(s) 334, 576, 958, 1164, 1267, 2902, 4107
SexAI ACCWGGT 2 cut(s) 2101, 2914
SfaNI GCATC 8 cut(s) 301, 422, 903, 924, 1551, 2298, 2694, 3013
SfcI CTRYAG 3 cut(s) 78, 1070, 2109
Sfr274I CTCGAG 1 cut(s) 1986
SinI GGWCC 8 cut(s) 944, 1145, 1377, 2131, 3179, 3483, 3649, 4062
SlaI CTCGAG 1 cut(s) 1986
SmiMI CAYNNNNRTG 8 cut(s) 362, 1153, 1781, 2060, 2504, 2636, 2676, 3821
SmlI CTYRAG 7 cut(s) 161, 233, 833, 848, 1745, 1835, 1986
SmoI CTYRAG 7 cut(s) 161, 233, 833, 848, 1745, 1835, 1986
SnaBI TACGTA 1 cut(s) 564
SseBI AGGCCT 1 cut(s) 3189
SsiI CCGC 3 cut(s) 1616, 2294, 4080
SspI AATATT 2 cut(s) 748, 1545
SspMI CTAG 8 cut(s) 129, 678, 726, 794, 1362, 1455, 2841, 3108
SstI GAGCTC 1 cut(s) 958
StuI AGGCCT 1 cut(s) 3189
StyI CCWWGG 5 cut(s) 151, 1893, 2118, 2280, 3915
TaiI ACGT 3 cut(s) 561, 566, 1270
TaqI TCGA 9 cut(s) 25, 144, 1101, 1371, 1987, 2481, 2812, 3831, 4010
TatI WGTACW 8 cut(s) 1842, 2272, 3213, 3333, 3414, 3726, 3873, 3888
TauI GCSGC 1 cut(s) 4082
TfiI GAWTC 5 cut(s) 1590, 2114, 2786, 3125, 3828
TscAI CASTG 6 cut(s) 339, 669, 3072, 3485, 3672, 4194
TseFI GTSAC 2 cut(s) 919, 1231
Tsp45I GTSAC 2 cut(s) 919, 1231
TspGWI ACGGA 2 cut(s) 1445, 2325
TspRI CASTG 6 cut(s) 339, 669, 3072, 3485, 3672, 4194
Van91I CCANNNNNTGG 1 cut(s) 3014
VpaK11BI GGWCC 8 cut(s) 944, 1145, 1377, 2131, 3179, 3483, 3649, 4062
VspI ATTAAT 1 cut(s) 3228
XagI CCTNNNNNAGG 2 cut(s) 1372, 3396
XapI RAATTY 4 cut(s) 587, 1175, 2069, 3655
XbaI TCTAGA 2 cut(s) 725, 1454
XceI RCATGY 1 cut(s) 2509
XcmI CCANNNNNNNNNTGG 4 cut(s) 508, 868, 2099, 2163
XhoI CTCGAG 1 cut(s) 1986
XmiI GTMKAC 1 cut(s) 3813
XmnI GAANNNNTTC 2 cut(s) 3983, 4137
XspI CTAG 8 cut(s) 129, 678, 726, 794, 1362, 1455, 2841, 3108
ZrmI AGTACT 2 cut(s) 2274, 3215
Zsp2I ATGCAT 2 cut(s) 1566, 3458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.