Rh6BG403100
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000493
Physical Location & Seq
Forward (+)
62975923 .. 62983217
7295 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG403100.1

Sequence Viewer

Length: 4356 bp
ATGGAGAGTAGTAGTGGAGATGTATATAGAGTGAGCAGTGCTCGTCTGAGCAGTTCAAACATTTGGAGGAACAGTACCATGGATGTTTTCTCCAAGTCTTCACACGATGAAGACGATGAAGAAGCGCTGAAATGGGCCGCCATTGAAAAACTGCCAACTTACTTGCGTATAAGGAGAGGCATTCTCACCGAGGAAGAAGGCGAAGCGAGAGAGATTGACATCAAGAATCTTGGATTGTTGGAAAGGAAGAATGTGTTGGAGAGGCTGGTAAAAGTTGCAGATGAAGATAATGAGAGGTTCTTGATGAAGCTCAAGGACCGGATTGATAGGGTTGGACTTGATATTCCGACGATTGAAGTCCGGTTTGAGCATTTGAATGTTGAAGCAGAAGCTTATGTAGGAGGGAGGGCGCTGCCTACGCTCTATAACTTTTCTGTTAATATCTTGGAGGGATTCCTGAGTTTCTGTCGCATTATTCCAACTAGAAAGCATCCATTACCAATCCTTGATGATGTTAGTGGAATTATCAAACCAAAAAGAATGACACTGCTTTTAGGCCCCCCAAACTCTGGAAAAACCACATTGCTTTTGGCTTTGGCTGGTAAACTTGCTAAAGATCTAAAGTCTTCTGGGAGAGTTGCATATAACGGACATGGGATGGAAGAGTTTGTACCGGAGAGGACATCAGCTTATATTAGTCAGTATGATCTCCACATAGGGGAAATGACAGTAAGAGAAACACTGGCCTTTTCAGCAAGGTGTCAAGGGGTTGGAGGACGTTATGAAATGTTGGCAGAGTTATCAAGGAGAGAAAAGGCAGCAAATATTATGCCAGAGAAAGATCTTGATATCTACATGAAGGCTGCATCATTAGAAGGGCAGGAGGCCAATGTAGTTACAGATTACATACTCAAGATTCTGGGACTTGAAGTTTGTGCTGACACCATGGTGGGGGATGAAATGTTCCGAGGTATATCTGGCGGACAAAAGAAGCGAGTCACAACAGGGGAAATGCTGGTTGGACCAGCAAGAGCACTTTTTATGGATGAGATATCAACTGGTTTAGACAGTTCAACAACATTTCAGATAGTGAATTCCCTCAGACAAACTATCCACATCCTCAATGGAACTGCTCTTATATCTCTTCTCCAGCCAGCACCGGAAACATATGACCTGTTTGATGACATAATTCTTCTGTCAGATGGGCAAATTGTATACCAAGGTCCCCGCGAAAATGTGCTCGAGTTCTTTGAGTATATGGGCTTCAAATGTCCAGAGAGGAAAGGAGTTGCTGACTTCCTTCAAGAAGTGACTTCAAAGAAGGATCAGGAGCAGTACTGGATACAGAAAGAAGAGCCTTATAATTTTATATCTTCCAAGGAATTTGCTGAAGCATTTCAGTCATTTCATATTGGTCGAAAACTAGGTGATGATCTTGCTACTCCATTTGACAAGTCCAAAGGTCACCCTGCTGCTTTAACAACTAAGAAGTATGGTGTTAGCAAGAGGGAACTGCTCAAAGCCTGCATTGCTAGAGAATATTTGCTGATGAAGAGGAATTCGTTTGTGTACATTTTCAAAATGACCCAGCTCACTCTATTGGCCTTTATATCAATGACCCTATTTTTCCGTACTAAGATGCACCGGGATACAGTTGAGGATGGTGGGATTTACATGGGTGCTATGTTCTTCACAGTCATCATAATTATGTTCAATGGGTTCTCCGAGCTTGCATTGACTATCATGAAACTTCCTGTATTTTTCAAGCAAAGAGACCTTCTGTTCTATCCAGCTTGGGCATATTCCTTACCAGCATGGATCCTTAAGATTCCCATCACCTTTGTAGAAGTTGCCATTTGGGTGTTCATGACATACTATGTAATTGGTTTTGATCCAAACCCCGGAAGGTTTTTCAAGCAATATCTTTTGCTCCTATGCATTAACCAGATGGCATCCGGATTATTCCGATTTATGGGGGCATTAGGGAGGAATATAATTGTTGCCAACACATTTGGTTCATTTGCTCTACTTGCAGTTCTGGTTATGGGTGGCTTTATCTTGTCCCGAGAGAATGTGCAGGCGTGGTGGTTATGGGGTTATTGGATCTCACCAATGATGTATGGCCAGAATGCTATAGCTGTCAATGAATTTCTTGGAGACAGTTGGAGTCATATTCCTCCTGACTCAACAGAGTCGTTAGGGATCATGGTCTTGAAGTCTCGTGGGGTTTTCGTACAAGCATATTGGTACTGGATTGGAGTAGGAGCTACAATTGGATATATATTTCTGTTTAATTTCTTCTACACTGTGGCCTTGCATTATCTTGATCCGTTTGGGAAACCTCAGGCTGTACTCTCCAAAGAAGCTTTAGCTGATAAAAACGCTGCTAGAGCTGGAGGAAACATAGAGCTATCATCAAGAGGAAAGAACTCTTCAGATGCAAAGAATGATAGTCGAAGAAGTATCTCATCTAGAACACTGTCTGCTAGAGTGGGCAGCTTTAATGAAGCCAATGAAAACAGGAAGAGGGGAATGGTTCTTCCTTTTGAACCCCTTTGGATTACATTTAATGAAATCAAATATTCTGTGGACATGCCCGAGGAAATGAAAACTCAAGGAATAACTGAGGAACGGTTACCACTTCTAAAGGGCGTGACTGGTGCTTTTAGGCCTGGAGTTTTAACAGCTCTAATGGGCATAAGTGGTGCTGGAAAGACTACTCTAATGGATGTCTTGGCAGGCAGAAAAACTGGTGGATATATTGAGGGGGACATAACCATTTCTGGGTATCCAAAAAAGCAAGAAACATTTGCTCGTATATCAGGATATTGTGAGCAAACCGATATACACTCTCCTCATGTTACAGTATATGAGTCTTTGGTTTATTCTGCATGGCTCCGGTTGCCCCCTGAGGTTGATTCCGCAACTAGAAAGATGTTTGTTGAGGAAGTCATGGAGCTTGTGGAACTGACGTCGATAAGGGAGTCTCTTGTGGGATTGCCAGGAGTAAATGGTCTCTCAACCGAGCAGCGTAAAAGGCTAACCATTGCTGTAGAACTTGTTGCTAACCCTTCCATAATATTTATGGATGAGCCAACTTCTGGTCTTGATGCAAGGGCAGCAGCTATTGTAATGAGAACAGTGAGGAATACTGTGGACACAGGGCGAACTGTAGTCTGCACTATCCACCAGCCAAGCATCGACATTTTTGATGCTTTTGATGAGCTGTTTCTATTGAAAAGGGGAGGTGAAGAGATATATGTTGGCCCTCTAGGCCGACATTCTTCCCAGTTGATCAAGTATTTTGAGGAAATTGATGGGGTTCCCAAAATCAAGGATGGTTACAATCCAGCAACTTGGATGTTGGAGGTTACTTCAGCAGCACAGGAAGTAGTTCTCGGTATTAACTTTACCGATATATACAGGAGCTCAGAGCTATATAGGAGAAACAAGGACCTGATCAAGGAACTGAGTACTCCTCCGCAAGGCACAAAAGACTTGTACTTCTCTACTAAATACTCTCAGTCTTTCTACTATCAGTGTATGGCTTGTTTATGGAAACAGCATCTATCATACTGGCGAAACCCACCATACAGTGCAGTGAGACTCCTATTCACCACTTTCATAGCCTTAATGTTTGGAACAATATTCTGGGATCTTGGCTCCAAAAGGAAAAACCAACAAGATCTATTCAATGCTATGGGTTCCATGTACGCAGCTGTTCTCTTCATTGGAGTACAAAATGCCTCATCGGTGCAGCCAGTTGTGGCTATCGAGAGGACAGTCTTTTACAGAGAAAGGGCTGCTGGAATGTACTCAGCATTGCCATATGCCTTCGGACAGGTTGTGATTGAGCTTCCATACATCTTTGTCCAGACTGTCATATATGGAGTTATAGTATACGCCATGATTGGATTTGACTGGACAGTGAGCAAGTTCCTTTGGTATCTCTTCTTCATGTACTTCACTTTCTTATACTTTACATTCTATGGGATGATGACCGTGGCTGTTACACCCAACCACAACATTGCCGCCATAGTGTCCTCTGCCTTCTATGCAATATGGAACCTTTTCTCAGGATTCATTGTTCCCCGAACAAGAATGCCAATTTGGTGGAGATGGTACTATTGGATCTGCCCGGTCTCATATACATTGTATGGACTTGTTGCTTCACAGTTTGGAGACATAAAAGAAGTAATGGATTCAGGTGAAAGTGTGGAAGACTTTGTGAGGAACTACTTTGGTTACAGACACGACTTTGTAGGTATTGTTGCAGTTGTGCTTGTTGGGATTTCAGTGCTTTTCGGGTTCACCTTTGCCTTCTCGATCAAGGCATTCAACTTCCAAAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1451

Amino Acids

164.45

Weight (kDa)

6.71

Isoelectric Point (pI)

40.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 94 - 144 6.6e-07 ABC-transporter N-terminal
ABC_tran PF00005 169 - 350 6.3e-14 ABC transporter
ABC2_membrane PF01061 505 - 717 1.2e-47 ABC-2 type transporter
PDR_assoc PF08370 722 - 784 1.1e-27 Plant PDR ABC transporter associated
ABC_tran PF00005 882 - 1034 5.8e-20 ABC transporter
ABC2_membrane PF01061 1179 - 1393 2.3e-64 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000493)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29820 FvH4_2g29820 FvH4_2g29822 FvH4_2g29824 FvH4_2g29824 FvH4_2g29824 FvH4_2g29840 FvH4_2g29840 FvH4_2g29840 FvH4_5g00550 FvH4_5g00550
malus_domestica MD02G1032700.v1.1 MD08G1140900.v1.1 MD08G1141400.v1.1 MD15G1117800.v1.1 MD15G1117900.v1.1
prunus_persica Prupe.1G471200_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471400_v2.0.a1 Prupe.7G242900_v2.0.a1
pyrus_communis pycom02g02690 pycom08g11890
rosa_chinensis RchiOBHm_Chr2g0111741 RchiOBHm_Chr2g0145991 RchiOBHm_Chr6g0298851 RchiOBHm_Chr6g0298871 RchiOBHm_Chr6g0298891 RchiOBHm_Chr6g0298901 RchiOBHm_Chr6g0298911 RchiOBHm_Chr7g0201801 RchiOBHm_Chr7g0211531 RchiOBHm_Chr7g0214601
rosa_laevigata RLG00000002931 RLG00000003000 RLG00000003647 RLG00000011476 RLG00000011479
rosa_multiflora Rmu_sc0001030.1_g000012 Rmu_sc0001548.1_g000041 Rmu_ssc0000397.1_g000033 Rmu_ssc0000397.1_g000036 Rmu_ssc0000397.1_g000037 Rmu_ssc0000397.1_g000039 Rmu_ssc0000397.1_g000048
rosa_roxburghii Rroxscaffold_1G00003830 Rroxscaffold_3G00247810 Rroxscaffold_3G00255060 Rroxscaffold_7G00169220 Rroxscaffold_7G00169230 Rroxscaffold_7G00169280
rosa_rugosa Rorug02G0223600 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283200 Rorug06G0283300 Rorug06G0283400 Rorug06G0283500 Rorug07G0066000
rosa_samantha Rh2AG511300 Rh2CG235100 Rh2CG496400 Rh2DG330900 Rh6BG403100 Rh6BG403300 Rh6BG403400 Rh6BG403500 Rh6CG408700 Rh6CG408900 Rh6CG409000 Rh6CG409200 Rh6DG395800 Rh6DG395900 Rh6DG396000 Rh6DG396200 Rh7AG193400 Rh7BG195100 Rh7BG436600 Rh7DG199300 Rh7DG265500
rosa_wichuraiana Rw2G016900 Rw6G034570 Rw6G034580 Rw6G034590 Rw6G034600 Rw7G016900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1362
AatII GACGTC 1 cut(s) 2975
AccB7I CCANNNNNTGG 2 cut(s) 569, 3101
AccI GTMKAC 2 cut(s) 1215, 3900
AccII CGCG 1 cut(s) 1230
AccIII TCCGGA 1 cut(s) 1955
AciI CCGC 6 cut(s) 138, 981, 1228, 2922, 3482, 4032
AclWI GGATC 9 cut(s) 1332, 1813, 1826, 1886, 2111, 2210, 2321, 3663, 4139
AcoI YGGCCR 1 cut(s) 2122
AcsI RAATTY 4 cut(s) 1093, 1382, 1558, 2147
AcuI CTGAAG 3 cut(s) 1410, 2418, 3360
AcyI GRCGYC 1 cut(s) 2972
AfeI AGCGCT 1 cut(s) 126
AfiI CCNNNNNNNGG 9 cut(s) 569, 718, 951, 1901, 1972, 2784, 3101, 3463, 3485
AflII CTTAAG 1 cut(s) 1823
AjnI CCWGG 2 cut(s) 2671, 3001
AjuI GAANNNNNNNTTGG 6 cut(s) 239, 271, 1002, 1034, 4093, 4125
AleI CACNNNNGTG 1 cut(s) 947
AloI GAACNNNNNNTCC 4 cut(s) 947, 979, 4071, 4103
Alw21I GWGCWC 4 cut(s) 43, 1036, 1242, 3431
Alw26I GTCTC 8 cut(s) 1767, 2151, 2223, 2991, 3020, 3598, 4147, 4176
AlwI GGATC 9 cut(s) 1332, 1813, 1826, 1886, 2111, 2210, 2321, 3663, 4139
Ama87I CYCGRG 3 cut(s) 1241, 2064, 2597
Aor13HI TCCGGA 1 cut(s) 1955
Aor51HI AGCGCT 1 cut(s) 126
ApoI RAATTY 4 cut(s) 1093, 1382, 1558, 2147
ArsI GACNNNNNNTTYG 2 cut(s) 915, 947
Asp700I GAANNNNTTC 3 cut(s) 1395, 3393, 4070
AspLEI GCGC 2 cut(s) 127, 412
AspS9I GGNCC 7 cut(s) 135, 316, 557, 1022, 1223, 3266, 3454
AsuC2I CCSGG 3 cut(s) 1646, 1902, 4139
AsuHPI GGTGA 9 cut(s) 178, 1439, 1457, 1828, 2100, 3260, 3607, 4220, 4303
AvaI CYCGRG 3 cut(s) 1241, 2064, 2597
AvaII GGWCC 4 cut(s) 316, 1022, 1223, 3454
AxyI CCTNAGG 2 cut(s) 2343, 2910
BalI TGGCCA 1 cut(s) 2124
BamHI GGATCC 1 cut(s) 1818
BanII GRGCYC 1 cut(s) 3431
BarI GAAGNNNNNNTAC 6 cut(s) 654, 686, 1247, 1279, 3938, 3970
BauI CACGAG 1 cut(s) 2220
BbsI GAAGAC 4 cut(s) 90, 117, 618, 4227
Bbv12I GWGCWC 4 cut(s) 43, 1036, 1242, 3431
BccI CCATC 8 cut(s) 652, 1196, 1655, 1841, 1942, 3311, 3332, 4113
BciT130I CCWGG 2 cut(s) 2673, 3003
BciVI GTATCC 3 cut(s) 1335, 1642, 2799
BclI TGATCA 2 cut(s) 3294, 3459
BcnI CCSGG 3 cut(s) 1646, 1902, 4139
BcoDI GTCTC 8 cut(s) 1767, 2151, 2223, 2991, 3020, 3598, 4147, 4176
BfaI CTAG 8 cut(s) 483, 1424, 1533, 2388, 2472, 2487, 2928, 3272
BfmI CTRYAG 3 cut(s) 2133, 3051, 3171
BfoI RGCGCY 2 cut(s) 128, 413
BfrI CTTAAG 1 cut(s) 1823
BfuI GTATCC 3 cut(s) 1335, 1642, 2799
BglI GCCNNNNNGGC 1 cut(s) 3273
BglII AGATCT 3 cut(s) 616, 841, 3685
BmcAI AGTACT 2 cut(s) 1337, 3475
Bme1390I CCNGG 5 cut(s) 1646, 1902, 2673, 3003, 4139
Bme18I GGWCC 4 cut(s) 316, 1022, 1223, 3454
BmeT110I CYCGRG 3 cut(s) 1241, 2064, 2597
BmgT120I GGNCC 7 cut(s) 135, 316, 557, 1022, 1223, 3266, 3454
BmiI GGNNCC 8 cut(s) 559, 1225, 1820, 2897, 3324, 3664, 3706, 4067
BmrFI CCNGG 5 cut(s) 1646, 1902, 2673, 3003, 4139
BmrI ACTGGG 1 cut(s) 3283
BmsI GCATC 9 cut(s) 499, 875, 1629, 1961, 2428, 3100, 3202, 3207, 3574
BmuI ACTGGG 1 cut(s) 3283
BpiI GAAGAC 4 cut(s) 90, 117, 618, 4227
BplI GAGNNNNNCTC 6 cut(s) 25, 57, 168, 200, 3463, 3495
BpmI CTGGAG 3 cut(s) 1133, 2415, 2694
BpuEI CTTGAG 3 cut(s) 296, 896, 2598
BpuMI CCSGG 3 cut(s) 1646, 1902, 4139
BsaBI GATNNNNATC 2 cut(s) 218, 1832
BsaHI GRCGYC 1 cut(s) 2972
BsaI GGTCTC 3 cut(s) 1767, 3020, 4147
BsaJI CCNNGG 9 cut(s) 78, 189, 945, 967, 1219, 1377, 1900, 2598, 4002
BsaWI WCCGGW 6 cut(s) 318, 360, 673, 1159, 1955, 2898
BsaXI ACNNNNNCTCC 4 cut(s) 58, 88, 3882, 3912
Bsc4I CCNNNNNNNGG 9 cut(s) 569, 718, 951, 1901, 1972, 2784, 3101, 3463, 3485
Bse21I CCTNAGG 2 cut(s) 2343, 2910
Bse3DI GCAATG 5 cut(s) 581, 1527, 3045, 3821, 4026
Bse8I GATNNNNATC 2 cut(s) 218, 1832
BseAI TCCGGA 1 cut(s) 1955
BseBI CCWGG 2 cut(s) 2673, 3003
BseDI CCNNGG 9 cut(s) 78, 189, 945, 967, 1219, 1377, 1900, 2598, 4002
BseJI GATNNNNATC 2 cut(s) 218, 1832
BseLI CCNNNNNNNGG 9 cut(s) 569, 718, 951, 1901, 1972, 2784, 3101, 3463, 3485
BseMI GCAATG 5 cut(s) 581, 1527, 3045, 3821, 4026
BseRI GAGGAG 2 cut(s) 2844, 3468
BseYI CCCAGC 1 cut(s) 1587
BsgI GTGCAG 4 cut(s) 2096, 3163, 3618, 3776
Bsh1236I CGCG 1 cut(s) 1230
BsiHKAI GWGCWC 4 cut(s) 43, 1036, 1242, 3431
BsiHKCI CYCGRG 3 cut(s) 1241, 2064, 2597
BsiSI CCGG 9 cut(s) 319, 361, 674, 1160, 1645, 1902, 1956, 2899, 4139
BslFI GGGAC 4 cut(s) 936, 1209, 2047, 2783
BslI CCNNNNNNNGG 9 cut(s) 569, 718, 951, 1901, 1972, 2784, 3101, 3463, 3485
BsmAI GTCTC 8 cut(s) 1767, 2151, 2223, 2991, 3020, 3598, 4147, 4176
BsmFI GGGAC 4 cut(s) 936, 1209, 2047, 2783
BsmI GAATGC 4 cut(s) 180, 2134, 4107, 4334
Bso31I GGTCTC 3 cut(s) 1767, 3020, 4147
BsoBI CYCGRG 3 cut(s) 1241, 2064, 2597
Bsp1286I GDGCHC 4 cut(s) 43, 1036, 1242, 3431
Bsp13I TCCGGA 1 cut(s) 1955
Bsp1407I TGTACA 1 cut(s) 1569
Bsp19I CCATGG 2 cut(s) 78, 945
BspACI CCGC 6 cut(s) 138, 981, 1228, 2922, 3482, 4032
BspEI TCCGGA 1 cut(s) 1955
BspFNI CGCG 1 cut(s) 1230
BspHI TCATGA 2 cut(s) 1743, 1866
BspLI GGNNCC 8 cut(s) 559, 1225, 1820, 2897, 3324, 3664, 3706, 4067
BspPI GGATC 9 cut(s) 1332, 1813, 1826, 1886, 2111, 2210, 2321, 3663, 4139
BspQI GCTCTTC 1 cut(s) 1347
BspTI CTTAAG 1 cut(s) 1823
BspTNI GGTCTC 3 cut(s) 1767, 3020, 4147
BsrDI GCAATG 5 cut(s) 581, 1527, 3045, 3821, 4026
BsrGI TGTACA 1 cut(s) 1569
BssECI CCNNGG 9 cut(s) 78, 189, 945, 967, 1219, 1377, 1900, 2598, 4002
BssNAI GTATAC 2 cut(s) 1216, 3901
BssNI GRCGYC 1 cut(s) 2972
BssSI CACGAG 1 cut(s) 2220
BssT1I CCWWGG 4 cut(s) 78, 945, 1219, 1377
Bst1107I GTATAC 2 cut(s) 1216, 3901
Bst2BI CACGAG 1 cut(s) 2220
Bst2UI CCWGG 2 cut(s) 2673, 3003
Bst6I CTCTTC 9 cut(s) 657, 1149, 1347, 1547, 2437, 2519, 3246, 3731, 3956
BstACI GRCGYC 1 cut(s) 2972
BstAFI CTTAAG 1 cut(s) 1823
BstAUI TGTACA 1 cut(s) 1569
BstC8I GCNNGC 5 cut(s) 1155, 1525, 1731, 2079, 2740
BstDSI CCRYGG 3 cut(s) 78, 945, 4002
BstEII GGTNACC 2 cut(s) 1463, 2634
BstENI CCTNNNNNAGG 2 cut(s) 3461, 3483
BstFNI CGCG 1 cut(s) 1230
BstH2I RGCGCY 2 cut(s) 128, 413
BstHHI GCGC 2 cut(s) 127, 412
BstMAI GTCTC 8 cut(s) 1767, 2151, 2223, 2991, 3020, 3598, 4147, 4176
BstNI CCWGG 2 cut(s) 2673, 3003
BstNSI RCATGY 1 cut(s) 2596
BstPI GGTNACC 2 cut(s) 1463, 2634
BstSCI CCNGG 5 cut(s) 1644, 1900, 2671, 3001, 4137
BstSFI CTRYAG 3 cut(s) 2133, 3051, 3171
BstUI CGCG 1 cut(s) 1230
BstV2I GAAGAC 4 cut(s) 90, 117, 618, 4227
BstX2I RGATCY 7 cut(s) 616, 841, 1818, 2103, 3655, 3685, 4131
BstXI CCANNNNNNTGG 2 cut(s) 3357, 4113
BstYI RGATCY 7 cut(s) 616, 841, 1818, 2103, 3655, 3685, 4131
BstZ17I GTATAC 2 cut(s) 1216, 3901
Bsu36I CCTNAGG 2 cut(s) 2343, 2910
BsuI GTATCC 3 cut(s) 1335, 1642, 2799
BtgI CCRYGG 3 cut(s) 78, 945, 4002
BtsI GCAGTG 3 cut(s) 43, 545, 3606
Cac8I GCNNGC 5 cut(s) 1155, 1525, 1731, 2079, 2740
CciI TCATGA 2 cut(s) 1743, 1866
CfoI GCGC 2 cut(s) 127, 412
Cfr13I GGNCC 7 cut(s) 135, 316, 557, 1022, 1223, 3266, 3454
EaeI YGGCCR 1 cut(s) 2122
Eam1104I CTCTTC 9 cut(s) 657, 1149, 1347, 1547, 2437, 2519, 3246, 3731, 3956
EarI CTCTTC 9 cut(s) 657, 1149, 1347, 1547, 2437, 2519, 3246, 3731, 3956
EciI GGCGGA 1 cut(s) 996
Ecl136II GAGCTC 1 cut(s) 3429
Eco130I CCWWGG 4 cut(s) 78, 945, 1219, 1377
Eco147I AGGCCT 1 cut(s) 2671
Eco24I GRGCYC 1 cut(s) 3431
Eco31I GGTCTC 3 cut(s) 1767, 3020, 4147
Eco32I GATATC 2 cut(s) 850, 1053
Eco47I GGWCC 4 cut(s) 316, 1022, 1223, 3454
Eco47III AGCGCT 1 cut(s) 126
Eco53kI GAGCTC 1 cut(s) 3429
Eco57I CTGAAG 3 cut(s) 1410, 2418, 3360
Eco81I CCTNAGG 2 cut(s) 2343, 2910
Eco88I CYCGRG 3 cut(s) 1241, 2064, 2597
Eco91I GGTNACC 2 cut(s) 1463, 2634
EcoICRI GAGCTC 1 cut(s) 3429
EcoNI CCTNNNNNAGG 2 cut(s) 3461, 3483
EcoO109I RGGNCCY 3 cut(s) 557, 1223, 3454
EcoO65I GGTNACC 2 cut(s) 1463, 2634
EcoRI GAATTC 2 cut(s) 1093, 1558
EcoRII CCWGG 2 cut(s) 2671, 3001
EcoRV GATATC 2 cut(s) 850, 1053
EcoT14I CCWWGG 4 cut(s) 78, 945, 1219, 1377
EcoT22I ATGCAT 1 cut(s) 1940
EcoT38I GRGCYC 1 cut(s) 3431
ErhI CCWWGG 4 cut(s) 78, 945, 1219, 1377
FaqI GGGAC 4 cut(s) 936, 1209, 2047, 2783
FauI CCCGC 1 cut(s) 1235
FauNDI CATATG 2 cut(s) 1168, 3829
FbaI TGATCA 2 cut(s) 3294, 3459
FblI GTMKAC 2 cut(s) 1215, 3900
FriOI GRGCYC 1 cut(s) 3431
FspBI CTAG 8 cut(s) 483, 1424, 1533, 2388, 2472, 2487, 2928, 3272
GlaI GCGC 2 cut(s) 126, 411
GsaI CCCAGC 1 cut(s) 1591
GsuI CTGGAG 3 cut(s) 1133, 2415, 2694
HaeII RGCGCY 2 cut(s) 128, 413
HapII CCGG 9 cut(s) 319, 361, 674, 1160, 1645, 1902, 1956, 2899, 4139
HhaI GCGC 2 cut(s) 127, 412
Hin1I GRCGYC 1 cut(s) 2972
Hin6I GCGC 2 cut(s) 125, 410
HinP1I GCGC 2 cut(s) 125, 410
HindIII AAGCTT 2 cut(s) 390, 2364
HpaII CCGG 9 cut(s) 319, 361, 674, 1160, 1645, 1902, 1956, 2899, 4139
HphI GGTGA 9 cut(s) 178, 1439, 1457, 1828, 2100, 3260, 3607, 4220, 4303
Hpy166II GTNNAC 7 cut(s) 605, 1216, 1570, 2590, 3157, 3901, 4311
Hpy8I GTNNAC 7 cut(s) 605, 1216, 1570, 2590, 3157, 3901, 4311
Hpy99I CGWCG 2 cut(s) 352, 2977
HpyCH4IV ACGT 2 cut(s) 778, 2972
HpySE526I ACGT 2 cut(s) 778, 2972
Hsp92I GRCGYC 1 cut(s) 2972
HspAI GCGC 2 cut(s) 125, 410
Kpn2I TCCGGA 1 cut(s) 1955
Ksp22I TGATCA 2 cut(s) 3294, 3459
LguI GCTCTTC 1 cut(s) 1347
LmnI GCTCC 7 cut(s) 1330, 1935, 2264, 2901, 2956, 3426, 3668
LweI GCATC 9 cut(s) 499, 875, 1629, 1961, 2428, 3100, 3202, 3207, 3574
MaeI CTAG 8 cut(s) 483, 1424, 1533, 2388, 2472, 2487, 2928, 3272
MaeII ACGT 2 cut(s) 778, 2972
MfeI CAATTG 1 cut(s) 2271
MflI RGATCY 7 cut(s) 616, 841, 1818, 2103, 3655, 3685, 4131
MhlI GDGCHC 4 cut(s) 43, 1036, 1242, 3431
MlsI TGGCCA 1 cut(s) 2124
MluNI TGGCCA 1 cut(s) 2124
MlyI GAGTC 7 cut(s) 1005, 2176, 2177, 2201, 2882, 2993, 3600
MmeI TCCRAC 9 cut(s) 219, 237, 313, 371, 503, 751, 1000, 2144, 3345
Mox20I TGGCCA 1 cut(s) 2124
Mph1103I ATGCAT 1 cut(s) 1940
MroI TCCGGA 1 cut(s) 1955
MroXI GAANNNNTTC 3 cut(s) 1395, 3393, 4070
MscI TGGCCA 1 cut(s) 2124
MslI CAYNNNNRTG 4 cut(s) 375, 947, 1706, 1859
Msp20I TGGCCA 1 cut(s) 2124
MspA1I CMGCKG 1 cut(s) 3719
MspCI CTTAAG 1 cut(s) 1823
MspI CCGG 9 cut(s) 319, 361, 674, 1160, 1645, 1902, 1956, 2899, 4139
MspR9I CCNGG 5 cut(s) 1646, 1902, 2673, 3003, 4139
MunI CAATTG 1 cut(s) 2271
Mva1269I GAATGC 4 cut(s) 180, 2134, 4107, 4334
MvaI CCWGG 2 cut(s) 2673, 3003
MvnI CGCG 1 cut(s) 1230
NciI CCSGG 3 cut(s) 1646, 1902, 4139
NcoI CCATGG 2 cut(s) 78, 945
NdeI CATATG 2 cut(s) 1168, 3829
NlaIV GGNNCC 8 cut(s) 559, 1225, 1820, 2897, 3324, 3664, 3706, 4067
NmuCI GTSAC 4 cut(s) 997, 1309, 1463, 2653
NsiI ATGCAT 1 cut(s) 1940
NspI RCATGY 1 cut(s) 2596
OliI CACNNNNGTG 1 cut(s) 947
PaeR7I CTCGAG 1 cut(s) 1241
PagI TCATGA 2 cut(s) 1743, 1866
PceI AGGCCT 1 cut(s) 2671
PciSI GCTCTTC 1 cut(s) 1347
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PctI GAATGC 4 cut(s) 180, 2134, 4107, 4334
PdmI GAANNNNTTC 3 cut(s) 1395, 3393, 4070
PfeI GAWTC 7 cut(s) 226, 453, 916, 1828, 2918, 4080, 4202
PflMI CCANNNNNTGG 2 cut(s) 569, 3101
PleI GAGTC 7 cut(s) 1004, 2175, 2177, 2200, 2881, 2992, 3600
PpsI GAGTC 7 cut(s) 1004, 2175, 2177, 2200, 2881, 2992, 3600
PpuMI RGGWCCY 2 cut(s) 1223, 3454
PsiI TTATAA 1 cut(s) 1362
Psp124BI GAGCTC 1 cut(s) 3431
Psp5II RGGWCCY 2 cut(s) 1223, 3454
Psp6I CCWGG 2 cut(s) 2671, 3001
PspEI GGTNACC 2 cut(s) 1463, 2634
PspFI CCCAGC 1 cut(s) 1587
PspGI CCWGG 2 cut(s) 2671, 3001
PspN4I GGNNCC 8 cut(s) 559, 1225, 1820, 2897, 3324, 3664, 3706, 4067
PspPI GGNCC 7 cut(s) 135, 316, 557, 1022, 1223, 3266, 3454
PspPPI RGGWCCY 2 cut(s) 1223, 3454
PspXI VCTCGAGB 1 cut(s) 1241
PsuI RGATCY 7 cut(s) 616, 841, 1818, 2103, 3655, 3685, 4131
PvuII CAGCTG 1 cut(s) 3719
RseI CAYNNNNRTG 4 cut(s) 375, 947, 1706, 1859
SacI GAGCTC 1 cut(s) 3431
SapI GCTCTTC 1 cut(s) 1347
Sau96I GGNCC 7 cut(s) 135, 316, 557, 1022, 1223, 3266, 3454
ScaI AGTACT 2 cut(s) 1337, 3475
SchI GAGTC 7 cut(s) 1005, 2176, 2177, 2201, 2882, 2993, 3600
ScrFI CCNGG 5 cut(s) 1646, 1902, 2673, 3003, 4139
SduI GDGCHC 4 cut(s) 43, 1036, 1242, 3431
SfaNI GCATC 9 cut(s) 499, 875, 1629, 1961, 2428, 3100, 3202, 3207, 3574
SfcI CTRYAG 3 cut(s) 2133, 3051, 3171
SfiI GGCCNNNNNGGCC 1 cut(s) 3273
Sfr274I CTCGAG 1 cut(s) 1241
SinI GGWCC 4 cut(s) 316, 1022, 1223, 3454
SlaI CTCGAG 1 cut(s) 1241
SmiMI CAYNNNNRTG 4 cut(s) 375, 947, 1706, 1859
SmlI CTYRAG 5 cut(s) 311, 911, 1241, 1823, 2613
SmoI CTYRAG 5 cut(s) 311, 911, 1241, 1823, 2613
SseBI AGGCCT 1 cut(s) 2671
SsiI CCGC 6 cut(s) 138, 981, 1228, 2922, 3482, 4032
SspI AATATT 5 cut(s) 826, 1541, 2582, 3081, 3648
SspMI CTAG 8 cut(s) 483, 1424, 1533, 2388, 2472, 2487, 2928, 3272
SstI GAGCTC 1 cut(s) 3431
StuI AGGCCT 1 cut(s) 2671
StyD4I CCNGG 5 cut(s) 1644, 1900, 2671, 3001, 4137
StyI CCWWGG 4 cut(s) 78, 945, 1219, 1377
TaiI ACGT 2 cut(s) 781, 2975
TaqI TCGA 7 cut(s) 1242, 1417, 2455, 2975, 3201, 3774, 4325
TatI WGTACW 8 cut(s) 1335, 1569, 2350, 3473, 3501, 3736, 3813, 3960
TauI GCSGC 2 cut(s) 140, 4034
TfiI GAWTC 7 cut(s) 226, 453, 916, 1828, 2918, 4080, 4202
TseFI GTSAC 4 cut(s) 997, 1309, 1463, 2653
Tsp45I GTSAC 4 cut(s) 997, 1309, 1463, 2653
TspGWI ACGGA 3 cut(s) 663, 1619, 2319
Van91I CCANNNNNTGG 2 cut(s) 569, 3101
Vha464I CTTAAG 1 cut(s) 1823
VpaK11BI GGWCC 4 cut(s) 316, 1022, 1223, 3454
XagI CCTNNNNNAGG 2 cut(s) 3461, 3483
XapI RAATTY 4 cut(s) 1093, 1382, 1558, 2147
XbaI TCTAGA 1 cut(s) 2471
XceI RCATGY 1 cut(s) 2596
XcmI CCANNNNNNNNNTGG 3 cut(s) 586, 1121, 3082
XhoI CTCGAG 1 cut(s) 1241
XmiI GTMKAC 2 cut(s) 1215, 3900
XmnI GAANNNNTTC 3 cut(s) 1395, 3393, 4070
XspI CTAG 8 cut(s) 483, 1424, 1533, 2388, 2472, 2487, 2928, 3272
ZraI GACGTC 1 cut(s) 2973
ZrmI AGTACT 2 cut(s) 1337, 3475
Zsp2I ATGCAT 1 cut(s) 1940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.