Rw6G034580
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000493
Physical Location & Seq
Forward (+)
59178150 .. 59187519
9370 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G034580.1

Sequence Viewer

Length: 4197 bp
ATGGAAGTTTTCTCCAAGTCTTCTCGTGAAGAAGACGATGAAGAAGCTCTGAAATGGGCTGCTATTGAGAGATTGCCAACTTACTTGCGCATAAGGAGAGGCATACTCACGGACCAAGACGCGGGTAAAGCCAGAGAAATTGATGTAAAAAATTTAGGACTTTTAGAAAGGAGGAATTTGCTGGAGAGGCTGGTGAAGATTGCAGATGAAGATAATGAGAAGTTCTTGTTCAAGATCAGGGACCGCATGGATAGAGTTGGACTTGAATTTCCAACAACTGAGGTGCGGTTTGACCATTTAAGTGTCGAAGCAGAAGCTTATGTAGGGAGTAGGGCATTACCTACAATGCTCAACTTCTCCATTAACATCTTAGAGGGGTTGTTGAATTACCTTCATGTTCTTCCAAGTAGAAAGAAGATATTGCCAATCCTCCACGGTGTTAGTGGAATTATCAAGCCAAGAAGAATGACACTGCTTTTAGGCCCTCCAAGCTCTGGAAAGACCACATTACTACTGGCTTTGGCTGGAAAACTCGGTAAAGATCTGAAACTTTCGGGGAGAGTTACATACAATGGAGAGGGGATGGAAGAGTTTGTACCAGAGAGGACATCAGCATATATTAGTCAACATGATCTTCACATACCAGAGATGACAAAATTTTCAGAAATGTTGGTAGAATTATCAAGGAGAGAAAAGACTGCAAATATAATGCCAGATCCTGATCTTGATGTTTACATGAAGGCAGCTGCACTAGAAGGACAGGAAACCAATGTAATTACAGATTATATACTCAAGGTTTTGGGGCTTGAAGTTTGTGCTGACACTATAGTAGGGGATCAAATGAGACGAGGTATCTCTGGGGGACAAAGAAAGCGACTCACCACAGGGGAGATGCTGGTTGGACCAGCAAGAGCACTTTTTATGGATGAGATATCCACTGGCTTGGACAGTTCAACAACATTTCAGATAGTGAAGTCACTTAGACAATCCATCCACATTCTCAATGGAACTGCTGTCATCTCTCTCCTCCAGCCAGCGCCAGAAACTTATGATCTTTTCGATGATATAATTCTACTCTCAGATGGGCACATTGTGTATCAAGGTCCCCGGGACAATGTGCTTGAGTTCTTTGAGTACATGGGCTTTAAATGTCCAGAGAGAAAAGGAGTTGCTGATTTTCTACAAGAAGTAACCTCAGGGAAAGATCAAGAGCAATACTGGGCTGATAAAGATAAGCCTTATAGCTTTGTAACTTCCAAGAACTTTGCTGAAGCCTTGCAATCATTTTACATTGGTCGAGAACTTGGTGATGAGCTTTCTATTCCGTTTGACAAGTCTAAAGGTCATCCTGCAGCTTTAACAACCAAGAAGTATGGCGTCAGCAAGAAGGAATTATTCAAAGCTTGTATGTCTAGGCAAATTCTGCTTATGAAGAGGAATTCATTTATCCACATTTTCAAAATTTCACAGATGATTCTATTGTCTTGTGTAACAATAACATTATTCCTACGAACTGAGATGCACCGAGATACTGTTGAAGATGGTGGGATATATATGGGAGCATTGTTTTACTCAATGATGATAATTATGTTCAATGGGTTCTCAGAGCTTGCTATGACTGTCATGAAGCTTCCTGTGTTTTTCAAGCAAAGGGACCTTCTCTTCTATCCTGCTTGGGCGTACTCTTTGCCCACATGGCTGATAAGGATCCCTATGACTTTTGTGGAAGTTTCCATTTGGGTGACCATTACGTACTACACTATAGGTTTTGATCCAAACATTGAAAGGTTCTTCAAACAGTTCCTTTCACTGCTGTTTATTAGCCAAATGGCAACTGGACTGTTCCGACTGATAGGGGCATTAGGAAGAAACATGATTGTTGCAAACACATTTGGATCGGTTGCTTTGCTTGTGCTGTTTGTTCTTGGTGGATTCCTTTTATCACGAGAGGATATGAAAAGCTGGTTGGTGTGGGGATATTATCTGTCACCAATGTCGTATGGACTGAATGCCCTGGCTGTCAATGAGTTTCTTGGACAAAGTTGGAGACATGTTTCTACCAACTCAAAAGAACCATTAGGAATAATGGTCTTGAATTCTCGAGGGGTGTTCCCAGAAGCACGTTGGTACTGGATTGGAGTAGTAGCTTTATTTGGATTTATTCTTCTATTCAACTTCCTTTTCATTTTGGCACTTCAGTATCTTGAACCTCTTGATAAGCCTCAGGCAATACTTTCCAAAGAGGCCTTGGCAAAGAAACAAGCCACTGAAACTGGAGAGGCCATTGAATTATTTTCAACAGGAAAGAGCTATATTGGTGCAGCTAATGAAAGACTAGACAATGTGTCGGTCAATCTTACTGGCCAGCGAGGAATGGTTCTTCCTTTTGAACCCCTCACAATTACATTTGATGAAATCAGATATGCAGTTGACATGCCACAGGAAATGAAAGCTCAAGGCATAACAGAGAATCGGTTAGAACTTCTGAAGGGTGTAAGTGGTGCTTTTAGGCCTGGAGTCCTAACAGCTCTAATGGGGGTCAGTGGCGCTGGAAAGACTACTCTAATGGATGTCTTGGCAGGAAGGAAAACTGGTGGACATATTGAAGGAAACATTAAAATTTCTGGCTATCCAAAGAAGCAAGAAACATTTGCTCGTATATCTGGATATTGTGAGCAAACGGATATACACTCTCCACATGTTACAGTCTATGAATCTTTGGTTTATTCTGCTTTGCTCCGGTTGCCCCCTGAGGTTGATTCCCCCACCAGAAAGATGTTTATTGAGGAAGTCATGGAACTTGTGGAACTGACCCCAATAAGAGATGCACTTGTTGGATTGCCTGGAGTGAATGGGCTCTCAACTGAGCAGCGCAAAAGACTAACAATCGCAGTAGAGCTTGTTGCCAACCCATCCATAATATTTATGGATGAGCCAACCTCTGGTCTTGATGCTAGGGCTGCAGCAATTGTAATGAGAACTGTGAGGAATACAGTGAACACCGGGAGAACTGTAGTCTGCACCATCCACCAGCCAAGCATTGATATATTTGATGCTTTTGATGAGATGCTACTTTTGAAATGGGGAGGAGAAGAAATATATGTTGGTCCTTTAGGCCACCAGTCCTCTCAGTTGATCGAATACTTTGAGGGGATCAGGGGAGTTCCTAAAATGAATGATGGTTACAATCCTGCAACTTGGATGTTAGAGGTTACTTCTGCAGCACAAGAATCAACTCTTGGGGTCAATTTCACTGACATATACAAGAACTCAGAAATGTACAGGAGAAACAAGGAATTGATCCAGGAACTAAGTACTCCACCAGAAAATTCAGTAGAATTGCACTTCACAACACAGTATTCTCAGTCCTTCTTTACCCAATGCATAACTTGCCTATGGAAACAACATCTGTCTTACTGGAGAAACCCGCCATACAGTGCAGCGAGACTTATTTACACAACTATGATGGCTATAGTATTTGGGATATTATTCTGGGGTCTTGGCTCCAAAAGGCAAAAGCAACAAGATCTTTTCAATGTATTAGGCTCTATGTATTCTGCCATACTCTTCATTGGGATACAAAATGCCTCATCAGTGCAGCCAGTTATAGCGATTGAGAGGGTAGTCTTTTACAGAGAAAGGGCAGCTGGAATGTACTCTGCTTTTCCATATGCATTTGGACAGGTTGTCATTGAGATACCATACACTTTGATCCAAACCACATTATATGTGGTTATAGTATACACCATGATCGGATATGATTGGAAGGTCAGCAAGTTCGTCTGGTATTTTTTCTTCATGTATTTCAGCTTCTTATACTACACATTATATGGTATGATGATTGTGGCCATTACTCCCAACACTATGGTTGCTGCTGTGCTTTCCTCATCCTTCTACCCGTTATGGAACGTCTTTTCTGGATTTATCATTCCCAAAACAAGAATTCCAATATGGTGGAGATGGTTCTATTGGTTGTGCCCGATTTCTTGGACCTTATACGGATTGATTGCTTCGCAGTTTGGAGACATTAAGGACGCACTTGAATCTGGCAAAACTGTGGAGAATTTTATTAGTGATTATTTTGGGTACAAAAAAGACTTTTTAGGTGTTGTTGCAATTGTGCATGTTGGGATTACAGTGCTCTTTGGTTTCATCTTTGCCTTTTCAATCAAAGCGTTTAATTTCCAAAGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1398

Amino Acids

158.5

Weight (kDa)

6.61

Isoelectric Point (pI)

41.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 69 - 119 3.5e-07 ABC-transporter N-terminal
ABC_tran PF00005 144 - 310 1.7e-13 ABC transporter
ABC2_membrane_7 PF19055 344 - 401 7.1e-06 ABC-2 type transporter
ABC2_membrane PF01061 465 - 677 7.3e-49 ABC-2 type transporter
PDR_assoc PF08370 682 - 744 1.8e-27 Plant PDR ABC transporter associated
ABC_tran PF00005 829 - 981 1.7e-20 ABC transporter
ABC2_membrane PF01061 1126 - 1340 2.2e-60 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000493)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29820 FvH4_2g29820 FvH4_2g29822 FvH4_2g29824 FvH4_2g29824 FvH4_2g29824 FvH4_2g29840 FvH4_2g29840 FvH4_2g29840 FvH4_5g00550 FvH4_5g00550
malus_domestica MD02G1032700.v1.1 MD08G1140900.v1.1 MD08G1141400.v1.1 MD15G1117800.v1.1 MD15G1117900.v1.1
prunus_persica Prupe.1G471200_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471400_v2.0.a1 Prupe.7G242900_v2.0.a1
pyrus_communis pycom02g02690 pycom08g11890
rosa_chinensis RchiOBHm_Chr2g0111741 RchiOBHm_Chr2g0145991 RchiOBHm_Chr6g0298851 RchiOBHm_Chr6g0298871 RchiOBHm_Chr6g0298891 RchiOBHm_Chr6g0298901 RchiOBHm_Chr6g0298911 RchiOBHm_Chr7g0201801 RchiOBHm_Chr7g0211531 RchiOBHm_Chr7g0214601
rosa_laevigata RLG00000002931 RLG00000003000 RLG00000003647 RLG00000011476 RLG00000011479
rosa_multiflora Rmu_sc0001030.1_g000012 Rmu_sc0001548.1_g000041 Rmu_ssc0000397.1_g000033 Rmu_ssc0000397.1_g000036 Rmu_ssc0000397.1_g000037 Rmu_ssc0000397.1_g000039 Rmu_ssc0000397.1_g000048
rosa_roxburghii Rroxscaffold_1G00003830 Rroxscaffold_3G00247810 Rroxscaffold_3G00255060 Rroxscaffold_7G00169220 Rroxscaffold_7G00169230 Rroxscaffold_7G00169280
rosa_rugosa Rorug02G0223600 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283200 Rorug06G0283300 Rorug06G0283400 Rorug06G0283500 Rorug07G0066000
rosa_samantha Rh2AG511300 Rh2CG235100 Rh2CG496400 Rh2DG330900 Rh6BG403100 Rh6BG403300 Rh6BG403400 Rh6BG403500 Rh6CG408700 Rh6CG408900 Rh6CG409000 Rh6CG409200 Rh6DG395800 Rh6DG395900 Rh6DG396000 Rh6DG396200 Rh7AG193400 Rh7BG195100 Rh7BG436600 Rh7DG199300 Rh7DG265500
rosa_wichuraiana Rw2G016900 Rw6G034570 Rw6G034580 Rw6G034590 Rw6G034600 Rw7G016900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 89
AccB7I CCANNNNNTGG 2 cut(s) 494, 2942
AccI GTMKAC 1 cut(s) 3741
AccII CGCG 1 cut(s) 122
AciI CCGC 4 cut(s) 122, 244, 286, 3428
AclWI GGATC 9 cut(s) 710, 843, 1702, 1715, 1766, 1903, 3161, 3295, 3706
AcoI YGGCCR 2 cut(s) 2362, 3846
AcuI CTGAAG 3 cut(s) 1290, 2180, 2507
AcyI GRCGYC 1 cut(s) 1377
AdeI CACNNNGTG 1 cut(s) 1093
AfaI GTAC 9 cut(s) 597, 1136, 1682, 1754, 2129, 3281, 3316, 3656, 4088
AfiI CCNNNNNNNGG 3 cut(s) 121, 494, 2942
AflIII ACRYGT 2 cut(s) 2050, 2698
AhdI GACNNNNNGTC 2 cut(s) 2344, 3686
AjnI CCWGG 4 cut(s) 2013, 2512, 2842, 3303
AjuI GAANNNNNNNTTGG 6 cut(s) 1949, 1981, 3087, 3119, 3222, 3254
Alw21I GWGCWC 2 cut(s) 916, 4143
Alw26I GTCTC 4 cut(s) 838, 2041, 3439, 4017
AlwI GGATC 9 cut(s) 710, 843, 1702, 1715, 1766, 1903, 3161, 3295, 3706
AlwNI CAGNNNCTG 1 cut(s) 719
Ama87I CYCGRG 2 cut(s) 1107, 2100
AoxI GGCC 7 cut(s) 481, 2244, 2280, 2362, 2510, 3115, 3846
AspLEI GCGC 4 cut(s) 90, 1039, 2549, 2874
AspS9I GGNCC 8 cut(s) 112, 241, 482, 902, 1103, 1654, 3107, 3990
AsuC2I CCSGG 3 cut(s) 1108, 1109, 3004
AsuHPI GGTGA 5 cut(s) 205, 871, 1319, 1753, 1980
AvaI CYCGRG 2 cut(s) 1107, 2100
AvaII GGWCC 7 cut(s) 112, 241, 902, 1103, 1654, 3107, 3990
AxyI CCTNAGG 3 cut(s) 1195, 2223, 2751
BaeGI GKGCMC 2 cut(s) 1089, 3980
BaeI ACNNNNGTAYC 2 cut(s) 2183, 2216
BalI TGGCCA 2 cut(s) 2364, 3848
BamHI GGATCC 1 cut(s) 1707
BanII GRGCYC 1 cut(s) 2859
BarI GAAGNNNNNNTAC 6 cut(s) 579, 611, 3779, 3794, 3811, 3826
BauI CACGAG 2 cut(s) 24, 1944
BbsI GAAGAC 2 cut(s) 12, 39
Bbv12I GWGCWC 2 cut(s) 916, 4143
BccI CCATC 9 cut(s) 577, 998, 1076, 1535, 2920, 3032, 3173, 3460, 3954
BciT130I CCWGG 4 cut(s) 2015, 2514, 2844, 3305
BciVI GTATCC 1 cut(s) 3570
BcnI CCSGG 3 cut(s) 1108, 1109, 3004
BcoDI GTCTC 4 cut(s) 838, 2041, 3439, 4017
BfaI CTAG 4 cut(s) 752, 1413, 2336, 2955
BfmI CTRYAG 7 cut(s) 825, 1350, 1761, 2961, 3012, 3219, 3471
BfoI RGCGCY 2 cut(s) 1040, 2550
BfuI GTATCC 1 cut(s) 3570
BglI GCCNNNNNGGC 1 cut(s) 1696
BglII AGATCT 2 cut(s) 541, 3526
BmcAI AGTACT 1 cut(s) 3316
Bme1390I CCNGG 7 cut(s) 1108, 1109, 2015, 2514, 2844, 3004, 3305
Bme18I GGWCC 7 cut(s) 112, 241, 902, 1103, 1654, 3107, 3990
BmeRI GACNNNNNGTC 2 cut(s) 2344, 3686
BmeT110I CYCGRG 2 cut(s) 1107, 2100
BmgT120I GGNCC 8 cut(s) 112, 241, 482, 902, 1103, 1654, 3107, 3990
BmiI GGNNCC 5 cut(s) 242, 1105, 1655, 1709, 3505
BmrFI CCNGG 7 cut(s) 1108, 1109, 2015, 2514, 2844, 3004, 3305
BmrI ACTGGG 1 cut(s) 1228
BmsI GCATC 6 cut(s) 882, 1509, 2815, 2941, 3043, 3057
BmuI ACTGGG 1 cut(s) 1228
BpiI GAAGAC 2 cut(s) 12, 39
BplI GAGNNNNNCTC 4 cut(s) 90, 122, 2924, 2956
BpmI CTGGAG 6 cut(s) 203, 1013, 2295, 2535, 2865, 3439
BpuEI CTTGAG 3 cut(s) 776, 1142, 2439
BpuMI CCSGG 3 cut(s) 1108, 1109, 3004
BsaAI YACGTR 1 cut(s) 1752
BsaBI GATNNNNATC 2 cut(s) 720, 1706
BsaHI GRCGYC 1 cut(s) 1377
BsaJI CCNNGG 5 cut(s) 433, 1106, 1107, 2013, 2247
BsaWI WCCGGW 1 cut(s) 2739
Bsc4I CCNNNNNNNGG 3 cut(s) 121, 494, 2942
Bse21I CCTNAGG 3 cut(s) 1195, 2223, 2751
Bse8I GATNNNNATC 2 cut(s) 720, 1706
BseBI CCWGG 4 cut(s) 2015, 2514, 2844, 3305
BseDI CCNNGG 5 cut(s) 433, 1106, 1107, 2013, 2247
BseJI GATNNNNATC 2 cut(s) 720, 1706
BseLI CCNNNNNNNGG 3 cut(s) 121, 494, 2942
BseRI GAGGAG 2 cut(s) 1016, 3102
BseSI GKGCMC 2 cut(s) 1089, 3980
BsgI GTGCAG 5 cut(s) 732, 2340, 3004, 3459, 3617
Bsh1236I CGCG 1 cut(s) 122
BshFI GGCC 7 cut(s) 483, 2246, 2282, 2364, 2512, 3117, 3848
BsiHKAI GWGCWC 2 cut(s) 916, 4143
BsiHKCI CYCGRG 2 cut(s) 1107, 2100
BsiSI CCGG 3 cut(s) 1108, 2740, 3003
BslFI GGGAC 5 cut(s) 254, 876, 1089, 1124, 1667
BslI CCNNNNNNNGG 3 cut(s) 121, 494, 2942
BsmAI GTCTC 4 cut(s) 838, 2041, 3439, 4017
BsmBI CGTCTC 1 cut(s) 838
BsmFI GGGAC 5 cut(s) 254, 876, 1089, 1124, 1667
BsmI GAATGC 1 cut(s) 2014
BsnI GGCC 7 cut(s) 483, 2246, 2282, 2364, 2512, 3117, 3848
BsoBI CYCGRG 2 cut(s) 1107, 2100
Bsp1286I GDGCHC 5 cut(s) 916, 1089, 2859, 3980, 4143
Bsp1407I TGTACA 1 cut(s) 3279
BspACI CCGC 4 cut(s) 122, 244, 286, 3428
BspANI GGCC 7 cut(s) 483, 2246, 2282, 2364, 2512, 3117, 3848
BspFNI CGCG 1 cut(s) 122
BspHI TCATGA 1 cut(s) 1623
BspLI GGNNCC 5 cut(s) 242, 1105, 1655, 1709, 3505
BspMAI CTGCAG 3 cut(s) 1354, 2965, 3223
BspPI GGATC 9 cut(s) 710, 843, 1702, 1715, 1766, 1903, 3161, 3295, 3706
BsrGI TGTACA 1 cut(s) 3279
BssECI CCNNGG 5 cut(s) 433, 1106, 1107, 2013, 2247
BssNAI GTATAC 1 cut(s) 3742
BssNI GRCGYC 1 cut(s) 1377
BssSI CACGAG 2 cut(s) 24, 1944
BssT1I CCWWGG 1 cut(s) 2247
Bst1107I GTATAC 1 cut(s) 3742
Bst2BI CACGAG 2 cut(s) 24, 1944
Bst2UI CCWGG 4 cut(s) 2015, 2514, 2844, 3305
Bst6I CTCTTC 4 cut(s) 582, 1427, 1667, 3572
BstACI GRCGYC 1 cut(s) 1377
BstAPI GCANNNNNTGC 2 cut(s) 1423, 3390
BstAUI TGTACA 1 cut(s) 3279
BstBAI YACGTR 1 cut(s) 1752
BstC8I GCNNGC 3 cut(s) 1035, 1611, 2366
BstDSI CCRYGG 1 cut(s) 433
BstEII GGTNACC 1 cut(s) 1741
BstFNI CGCG 1 cut(s) 122
BstH2I RGCGCY 2 cut(s) 1040, 2550
BstHHI GCGC 4 cut(s) 90, 1039, 2549, 2874
BstMAI GTCTC 4 cut(s) 838, 2041, 3439, 4017
BstMWI GCNNNNNNNGC 8 cut(s) 128, 187, 489, 1423, 1696, 2743, 2960, 3390
BstNI CCWGG 4 cut(s) 2015, 2514, 2844, 3305
BstNSI RCATGY 4 cut(s) 2054, 2437, 2702, 4127
BstPI GGTNACC 1 cut(s) 1741
BstSCI CCNGG 7 cut(s) 1106, 1107, 2013, 2512, 2842, 3002, 3303
BstSFI CTRYAG 7 cut(s) 825, 1350, 1761, 2961, 3012, 3219, 3471
BstSLI GKGCMC 2 cut(s) 1089, 3980
BstSNI TACGTA 1 cut(s) 1752
BstUI CGCG 1 cut(s) 122
BstV2I GAAGAC 2 cut(s) 12, 39
BstX2I RGATCY 4 cut(s) 541, 715, 1707, 3526
BstXI CCANNNNNNTGG 3 cut(s) 943, 3865, 3954
BstYI RGATCY 4 cut(s) 541, 715, 1707, 3526
BstZ17I GTATAC 1 cut(s) 3742
Bsu36I CCTNAGG 3 cut(s) 1195, 2223, 2751
BsuI GTATCC 1 cut(s) 3570
BsuRI GGCC 7 cut(s) 483, 2246, 2282, 2364, 2512, 3117, 3848
BtgI CCRYGG 1 cut(s) 433
BtsI GCAGTG 2 cut(s) 470, 1808
Cac8I GCNNGC 3 cut(s) 1035, 1611, 2366
CaiI CAGNNNCTG 1 cut(s) 719
CciI TCATGA 1 cut(s) 1623
CfoI GCGC 4 cut(s) 90, 1039, 2549, 2874
Cfr13I GGNCC 8 cut(s) 112, 241, 482, 902, 1103, 1654, 3107, 3990
Cfr9I CCCGGG 1 cut(s) 1107
CseI GACGC 3 cut(s) 128, 1366, 4043
Csp6I GTAC 9 cut(s) 596, 1135, 1681, 1753, 2128, 3280, 3315, 3655, 4087
CviQI GTAC 9 cut(s) 596, 1135, 1681, 1753, 2128, 3280, 3315, 3655, 4087
DraI TTTAAA 1 cut(s) 1147
DraIII CACNNNGTG 1 cut(s) 1093
DriI GACNNNNNGTC 2 cut(s) 2344, 3686
EaeI YGGCCR 2 cut(s) 2362, 3846
Eam1104I CTCTTC 4 cut(s) 582, 1427, 1667, 3572
Eam1105I GACNNNNNGTC 2 cut(s) 2344, 3686
EarI CTCTTC 4 cut(s) 582, 1427, 1667, 3572
Eco105I TACGTA 1 cut(s) 1752
Eco130I CCWWGG 1 cut(s) 2247
Eco147I AGGCCT 2 cut(s) 2246, 2512
Eco24I GRGCYC 1 cut(s) 2859
Eco32I GATATC 1 cut(s) 933
Eco47I GGWCC 7 cut(s) 112, 241, 902, 1103, 1654, 3107, 3990
Eco57I CTGAAG 3 cut(s) 1290, 2180, 2507
Eco81I CCTNAGG 3 cut(s) 1195, 2223, 2751
Eco88I CYCGRG 2 cut(s) 1107, 2100
Eco91I GGTNACC 1 cut(s) 1741
EcoO109I RGGNCCY 3 cut(s) 482, 1103, 1654
EcoO65I GGTNACC 1 cut(s) 1741
EcoRI GAATTC 3 cut(s) 1438, 2095, 3942
EcoRII CCWGG 4 cut(s) 2013, 2512, 2842, 3303
EcoRV GATATC 1 cut(s) 933
EcoT14I CCWWGG 1 cut(s) 2247
EcoT22I ATGCAT 2 cut(s) 3386, 3676
EcoT38I GRGCYC 1 cut(s) 2859
ErhI CCWWGG 1 cut(s) 2247
Esp3I CGTCTC 1 cut(s) 838
FalI AAGNNNNNCTT 2 cut(s) 2488, 2520
FaqI GGGAC 5 cut(s) 254, 876, 1089, 1124, 1667
FauI CCCGC 2 cut(s) 115, 3435
FauNDI CATATG 1 cut(s) 3670
FblI GTMKAC 1 cut(s) 3741
FriOI GRGCYC 1 cut(s) 2859
FspBI CTAG 4 cut(s) 752, 1413, 2336, 2955
FspI TGCGCA 1 cut(s) 89
GlaI GCGC 4 cut(s) 89, 1038, 2548, 2873
GsuI CTGGAG 6 cut(s) 203, 1013, 2295, 2535, 2865, 3439
HaeII RGCGCY 2 cut(s) 1040, 2550
HaeIII GGCC 7 cut(s) 483, 2246, 2282, 2364, 2512, 3117, 3848
HapII CCGG 3 cut(s) 1108, 2740, 3003
HgaI GACGC 3 cut(s) 128, 1366, 4043
HhaI GCGC 4 cut(s) 90, 1039, 2549, 2874
Hin1I GRCGYC 1 cut(s) 1377
Hin6I GCGC 4 cut(s) 88, 1037, 2547, 2872
HinP1I GCGC 4 cut(s) 88, 1037, 2547, 2872
HincII GTYRAC 2 cut(s) 626, 2431
HindII GTYRAC 2 cut(s) 626, 2431
HindIII AAGCTT 3 cut(s) 315, 1401, 1628
HinfI GANTC 9 cut(s) 876, 1474, 1932, 2470, 2517, 2714, 2759, 3230, 4043
HpaII CCGG 3 cut(s) 1108, 2740, 3003
HphI GGTGA 5 cut(s) 205, 871, 1319, 1753, 1980
Hpy166II GTNNAC 6 cut(s) 626, 733, 2431, 2597, 2998, 3742
Hpy8I GTNNAC 6 cut(s) 626, 733, 2431, 2597, 2998, 3742
HpyCH4IV ACGT 3 cut(s) 1751, 2122, 3909
HpyF10VI GCNNNNNNNGC 8 cut(s) 128, 187, 489, 1423, 1696, 2743, 2960, 3390
HpySE526I ACGT 3 cut(s) 1751, 2122, 3909
Hsp92I GRCGYC 1 cut(s) 1377
HspAI GCGC 4 cut(s) 88, 1037, 2547, 2872
LmnI GCTCC 3 cut(s) 1559, 2742, 3509
LweI GCATC 6 cut(s) 882, 1509, 2815, 2941, 3043, 3057
MaeI CTAG 4 cut(s) 752, 1413, 2336, 2955
MaeII ACGT 3 cut(s) 1751, 2122, 3909
MfeI CAATTG 2 cut(s) 2967, 4116
MflI RGATCY 4 cut(s) 541, 715, 1707, 3526
MhlI GDGCHC 5 cut(s) 916, 1089, 2859, 3980, 4143
MlsI TGGCCA 2 cut(s) 2364, 3848
MluNI TGGCCA 2 cut(s) 2364, 3848
MlyI GAGTC 2 cut(s) 870, 2526
MmeI TCCRAC 6 cut(s) 238, 296, 880, 1870, 2024, 2815
Mox20I TGGCCA 2 cut(s) 2364, 3848
Mph1103I ATGCAT 2 cut(s) 3386, 3676
MscI TGGCCA 2 cut(s) 2364, 3848
MseI TTAA 7 cut(s) 299, 363, 1146, 1358, 2616, 4029, 4179
MslI CAYNNNNRTG 3 cut(s) 300, 1739, 3461
Msp20I TGGCCA 2 cut(s) 2364, 3848
MspA1I CMGCKG 2 cut(s) 746, 3647
MspI CCGG 3 cut(s) 1108, 2740, 3003
MspR9I CCNGG 7 cut(s) 1108, 1109, 2015, 2514, 2844, 3004, 3305
MunI CAATTG 2 cut(s) 2967, 4116
Mva1269I GAATGC 1 cut(s) 2014
MvaI CCWGG 4 cut(s) 2015, 2514, 2844, 3305
MvnI CGCG 1 cut(s) 122
MwoI GCNNNNNNNGC 8 cut(s) 128, 187, 489, 1423, 1696, 2743, 2960, 3390
NciI CCSGG 3 cut(s) 1108, 1109, 3004
NdeI CATATG 1 cut(s) 3670
NlaIV GGNNCC 5 cut(s) 242, 1105, 1655, 1709, 3505
NmuCI GTSAC 3 cut(s) 975, 1741, 1986
NsbI TGCGCA 1 cut(s) 89
NsiI ATGCAT 2 cut(s) 3386, 3676
NspI RCATGY 4 cut(s) 2054, 2437, 2702, 4127
PaeR7I CTCGAG 1 cut(s) 2100
PagI TCATGA 1 cut(s) 1623
PceI AGGCCT 2 cut(s) 2246, 2512
PciI ACATGT 2 cut(s) 2050, 2698
PctI GAATGC 1 cut(s) 2014
PfeI GAWTC 7 cut(s) 1474, 1932, 2470, 2714, 2759, 3230, 4043
PflMI CCANNNNNTGG 2 cut(s) 494, 2942
PfoI TCCNGGA 1 cut(s) 3303
PleI GAGTC 2 cut(s) 870, 2525
PpsI GAGTC 2 cut(s) 870, 2525
Ppu21I YACGTR 1 cut(s) 1752
PpuMI RGGWCCY 2 cut(s) 1103, 1654
PscI ACATGT 2 cut(s) 2050, 2698
Psp5II RGGWCCY 2 cut(s) 1103, 1654
Psp6I CCWGG 4 cut(s) 2013, 2512, 2842, 3303
PspEI GGTNACC 1 cut(s) 1741
PspGI CCWGG 4 cut(s) 2013, 2512, 2842, 3303
PspN4I GGNNCC 5 cut(s) 242, 1105, 1655, 1709, 3505
PspPI GGNCC 8 cut(s) 112, 241, 482, 902, 1103, 1654, 3107, 3990
PspPPI RGGWCCY 2 cut(s) 1103, 1654
PstI CTGCAG 3 cut(s) 1354, 2965, 3223
PstNI CAGNNNCTG 1 cut(s) 719
PsuI RGATCY 4 cut(s) 541, 715, 1707, 3526
PvuII CAGCTG 2 cut(s) 746, 3647
RsaI GTAC 9 cut(s) 597, 1136, 1682, 1754, 2129, 3281, 3316, 3656, 4088
RsaNI GTAC 9 cut(s) 596, 1135, 1681, 1753, 2128, 3280, 3315, 3655, 4087
RseI CAYNNNNRTG 3 cut(s) 300, 1739, 3461
SaqAI TTAA 7 cut(s) 299, 363, 1146, 1358, 2616, 4029, 4179
Sau96I GGNCC 8 cut(s) 112, 241, 482, 902, 1103, 1654, 3107, 3990
ScaI AGTACT 1 cut(s) 3316
SchI GAGTC 2 cut(s) 870, 2526
ScrFI CCNGG 7 cut(s) 1108, 1109, 2015, 2514, 2844, 3004, 3305
SduI GDGCHC 5 cut(s) 916, 1089, 2859, 3980, 4143
SfaNI GCATC 6 cut(s) 882, 1509, 2815, 2941, 3043, 3057
SfcI CTRYAG 7 cut(s) 825, 1350, 1761, 2961, 3012, 3219, 3471
Sfr274I CTCGAG 1 cut(s) 2100
SinI GGWCC 7 cut(s) 112, 241, 902, 1103, 1654, 3107, 3990
SlaI CTCGAG 1 cut(s) 2100
SmaI CCCGGG 1 cut(s) 1109
SmiMI CAYNNNNRTG 3 cut(s) 300, 1739, 3461
SmlI CTYRAG 4 cut(s) 791, 1121, 2100, 2454
SmoI CTYRAG 4 cut(s) 791, 1121, 2100, 2454
SnaBI TACGTA 1 cut(s) 1752
SseBI AGGCCT 2 cut(s) 2246, 2512
SsiI CCGC 4 cut(s) 122, 244, 286, 3428
SspI AATATT 1 cut(s) 2922
SspMI CTAG 4 cut(s) 752, 1413, 2336, 2955
StuI AGGCCT 2 cut(s) 2246, 2512
StyD4I CCNGG 7 cut(s) 1106, 1107, 2013, 2512, 2842, 3002, 3303
StyI CCWWGG 1 cut(s) 2247
TaiI ACGT 3 cut(s) 1754, 2125, 3912
TaqI TCGA 5 cut(s) 306, 1059, 1297, 2101, 3138
TaqII GACCGA 1 cut(s) 2338
TatI WGTACW 4 cut(s) 1134, 3279, 3314, 3654
TfiI GAWTC 7 cut(s) 1474, 1932, 2470, 2714, 2759, 3230, 4043
Tru1I TTAA 7 cut(s) 299, 363, 1146, 1358, 2616, 4029, 4179
Tru9I TTAA 7 cut(s) 299, 363, 1146, 1358, 2616, 4029, 4179
TseFI GTSAC 3 cut(s) 975, 1741, 1986
Tsp45I GTSAC 3 cut(s) 975, 1741, 1986
TspGWI ACGGA 4 cut(s) 125, 1314, 2696, 4014
TspMI CCCGGG 1 cut(s) 1107
Van91I CCANNNNNTGG 2 cut(s) 494, 2942
VpaK11BI GGWCC 7 cut(s) 112, 241, 902, 1103, 1654, 3107, 3990
XceI RCATGY 4 cut(s) 2054, 2437, 2702, 4127
XcmI CCANNNNNNNNNTGG 8 cut(s) 440, 511, 1001, 1832, 2121, 2245, 2923, 3727
XhoI CTCGAG 1 cut(s) 2100
XmaI CCCGGG 1 cut(s) 1107
XmiI GTMKAC 1 cut(s) 3741
XspI CTAG 4 cut(s) 752, 1413, 2336, 2955
ZrmI AGTACT 1 cut(s) 3316
Zsp2I ATGCAT 2 cut(s) 3386, 3676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.