Rw6G034590
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000493
Physical Location & Seq
Forward (+)
59191302 .. 59199659
8358 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G034590.1

Sequence Viewer

Length: 4194 bp
ATGTGGAGAAACGAGGATACTTCAATGGAAGCTTTCTCCAAGTCTTCTCGTGAAGAAGACGATGAAGAATCTCTCAAATGGGCTGCTATTGAGAGATTGCCTACTTATTTACGCATAAGGAGAAGTATACTCGCTGATCAAGAGAAGGGTAAAGCCAGAGAAATTGATGTCAAGGATCTTGGATTACTAGAGAGGAAGAATTTGTTGGAGCGGTTAGTGAAGATTGCTGAGGAAGATAATGAGAAATTCTTGTTCAAGCTCAGGGACCGCATGAATAGAGTTGGACTTGAATTTCCAACAACTGAAGTGCGGTTCGAGCACTTAAATGTTGAAGCAGAAGCTTATGTAGGAAGCAGGGCATTACCTACAATGTTCAACTTCTCCATTAATATGTTAGAGGGGCTCTTGAATTGTGTTCATCTTCTTCCTAGTAGAAAGAAACCTTTGCCAATCCTACATGGTGTAAGTGGAACAATCAAGCCAAGAAGAATGACACTGCTTTTAGGCCCTCCAGGCTCCGGAAAATCCACATTGCTATTGGCATTGGCTGGAAAGCTCGGTAAAGATCTTAAAAGATCTGGAAGAGTTACCTACAATGGACTTGGGATGGATGAGTTTGTACCAGAGAGGACAGCAGCTTACATTAGTCAATATGATCTTCACATACCGGAGTTGACTGTGAGAGAAACTCTAGCTTTTTCAGCAAGATGTCAAGGGGTCGGACCACGTTATGAAATGTTAGTAGAACTACTAAGAAGAGAGAAGGCTGCAAATATTATGCCAGATTCTGATCTTGATGTCTACATGAAGGCAGCAGCACTAAAAGGACAGGAAACCACTGTGATTACAGATTATATACTCAAGGTTTTGGGACTTGAAGTTTGTGCAGACACCATGGTAGGGGATCAAATGAGACGAGGTATATCTGGTGGCCAAAGAAAGCGACTCACCACAGGGGAAATGCTTGTTGGGCCAGCAAGAGCACTTTTTATGGATGAGATATCGACTGGCCTGGACAGTTCAACAACATTTCAGATAGTAAATTCACTAAGACAATCCATTCACATTCTCAATGGAACTGCTGTAACCTCTCTCCTGCAGCCAGCACCAGAAACTTATGATCTCTTTGATGATATAATTCTCCTCTCAGATGGGCACATTGTGTATCAAGGTCCTCGTGAAAATGTGCTTGAGTTCTTTGAGTACATGGGCTTCCGATGTCCAGAGAGGAAAGGAGTCGCTGATTTCCTCCAAGAAGTGACATCATGGAAAGACCAAGAGCAATATTGGACTGATAAAGATAAGCCTTATAGCTTTGTCACGTCCAAGGAATTTTCTGTAGCATTGCAGTCATTTCACATTGGTCAAGAACTCGGTGATGAGCTTGCTATACCTTTTGACAAGTCTAGAGGAAACCCTGCATCTTTAGCAACTGAGAAGTACGGCGTTAGCAAGAAGGAACTTTTTAAAGCTTGTCTGTCTAGACATTATTTGCTCATGAAGAGGAACTCGTTTGTCTACATTACCAGAATGACAATGTTTCTCATAACTGCTTTTGTGACAATGACACTATTCCTACGGACTGAGATGCACCGGGATACAGTAGAAGATGGTGGCATTTATATGGGAGCTATGTTCTTCACAATGATGGTAATTATGTTCAATGGATACTCAGACCTTGCTATGACTCTCATGAAACTTCCTGTGTTTTACAAGCAAAGGGACCTGCTCTTCTATCCTGCTTGGGCATACTCTTTACCAACAGTGATTATAAGAATCCCCATGACCTTTGTGGAAGTTTCCATTTGGATGGCCATCACCTACTACGGTATTGGTTATGATCCAAGCATTGGAAGGTTCTGCAAGCAGTTCCTTGTACTAGTGTGCATTAGCCAGATGGCAAACGGACTGTTCCGATTGATAGGTGCATTAGGAAGGAACATGACTGTTGCAAACACATTTGGATCTGTTGCTTTACTATGCTGTTTTGTTCTGGGTGGCTTTATGTTATCACGAGAGGAGATGAAGAACTGGTTGTTGTGGGGATATTGGCTCTCACCGATGACGTATGGAACGAATGCAATGGCTGTCAATGAGTTTTTTGGAAAAAGTTGGAGACATGTTCCACCTAACTCAACAGAATCGTTAGGAGTTATGGTCTTGAAGTCTCGAGGGGTATTCCCAGATGAAAGTTGGTACTGGATTGGAGTAGTAGCTTTATTTGGATATTTTCTTCTGTTCAACTTCCTTTACACTTTGGCACTTCAGCTTCTGGAACCACTTGATAAGCCTCAGGCAGTATTGTCCAAAGAGGCTTTGGCAGTGAAACACGCCATGAAAACTGGAGAGACCATTGAGCTATCGCCAATAGGAAACAATTCTATTGGAGTAGGGAATGAAGTTCTAGAGAATGTGTCACCCAATCGTCCCATGCAGCAAGGAATGATTCTTCCTTTTCAACCCCTCTCAATTACGTTTACTGAAATCAAATATGCAGTTGACATGCCACAGGAAATGAAAGGTCAAGGCATTACTGAGGATCGACTAGAACTGCTGAAGGGTGTGAGTGGTGCTTTCAGGCCTGGGGTCCTAACAGCTCTAATGGGTGTTAGCGGTGCTGGAAAGACTACTTTACTGGATGTTCTGGCAGGAAGGAAAACCGGTGGCTATATTGAAGGAAACATTACCATTTCTGGGTATCCAAAAAAGCAAGAAACATTTGCTCGTATCTCAGGATATTGTGAGCAGACAGATATACACTCTCCACATGTTACGGTTTATGAGTCTTTGATTTATTCTGCATGGCTCCGCTTGTCCCCTGAAGTTGATTCCCCAACCAGAAAGATGTTTATTGAAGAAGCCATGGAACTTGTGGAACTGACCTCAATAAGGGAAGCACTTGTTGGATTGCCTGGAGGGAATGGTCTCTCGACTGAGCAGCGCAAAAGGCTAACAATTGCAGTAGAGCTTGTTGCCAACCCATCCATAATATTTATGGATGAGCCCACCTCTGGCCTTGATGCCAGGGCAGCAGCAATTGTAATGAGAACAGTGAGGAATACAGTGGATACCGGAAGAACAGTAGTCTGCACCATCCACCAGCCAAGCATTGATATATTTGATGCTTTTGATGAGATGCTCCTTCTGAAACAGGGTGGCGAAGAAATCTACGTTGGCCCTTTAGGCTACCATTCTTCCTACTTGATCGGATACTTTGAGGGAATTAGTGGAGTTTCTAAAATGAAGAATGGTTACAATCCTGCAACTTGGATGTTGGAGGTTACTTCTGCAGCTCAAGAAGCAGCTCTAGGGATTAATTTCACAGACATATACAAGAATTCAGAAGCATACAGGAGAAACAAGGCCTTGGTCAAGGAACTAAGTATTCCTCCACCGAATACAAGAGACTTTTACTTCCCAACAAAATATTCTCAGTCTTTCTATACCCAGTGCAAAGCTTGCTTATGGAAGCAACATTTGTCTTACTGGAGAAACCCCGCATATGTGCAGCAAAAGCAGCAAGATCTTTTTAATGCAATGGGTGCTATGTATTCTGCTATTCTCTTCATTGGGCCACAAAATACCTCAACAGTGCAGCCAGTTGTAGGCATTGAGCGAATAGTCTTTTACAGAGAAAGGGCTGCCGGAATGTACTCTGCCTTGCCATATGCCTTTGGACAGGTTGGGATTGAGCTCCCATACACTTTGATCCAAACTATCATATATGTGGTTATATCATACACGATGATCGGATTTGAGTGGACGGTCAGCAAGTTCTTTTGGCAACTATTCTTCATATACTTCACTTACTTATACTTCACCTTATATGGCATGATGAGTGTGGCCATTACTCCCAACATTGCCGTTTCCGCTGTAGTTTCCACGGCATTCAACCCGTTATGGAACGTTTTTTCAGGATTTATCATTCCCAAATCAAGAATTCCGATATGGTGGAGATGGTTCTACTGGTCGTGCCCGGTCTCTTGGAGCTTGTACGGAATGATTGGTTCACAGTTTGAAGGCATTGAGGACAGGCTTGATTCCGGCGAAACTGTGGACGCTTTTACCAGGACTTATTTTGGGTATAGAGAAGACTTTATGAGTGTTGTTGCAATAGTGCTTGTAGCTTTTTCAGTGCTCTTTGGATCCGTCTTTGCCTTTTCAATCAAAGCCTTTAACTTTCAAAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1397

Amino Acids

157.96

Weight (kDa)

6.54

Isoelectric Point (pI)

40.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 77 - 127 1.9e-07 ABC-transporter N-terminal
ABC_tran PF00005 152 - 333 2.6e-16 ABC transporter
ABC2_membrane_7 PF19055 367 - 447 9.7e-06 ABC-2 type transporter
ABC2_membrane PF01061 488 - 700 5e-49 ABC-2 type transporter
PDR_assoc PF08370 705 - 767 2.7e-26 Plant PDR ABC transporter associated
ABC_tran PF00005 852 - 1004 1.1e-20 ABC transporter
ABC2_membrane PF01061 1162 - 1339 1.7e-42 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000493)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29820 FvH4_2g29820 FvH4_2g29822 FvH4_2g29824 FvH4_2g29824 FvH4_2g29824 FvH4_2g29840 FvH4_2g29840 FvH4_2g29840 FvH4_5g00550 FvH4_5g00550
malus_domestica MD02G1032700.v1.1 MD08G1140900.v1.1 MD08G1141400.v1.1 MD15G1117800.v1.1 MD15G1117900.v1.1
prunus_persica Prupe.1G471200_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471400_v2.0.a1 Prupe.7G242900_v2.0.a1
pyrus_communis pycom02g02690 pycom08g11890
rosa_chinensis RchiOBHm_Chr2g0111741 RchiOBHm_Chr2g0145991 RchiOBHm_Chr6g0298851 RchiOBHm_Chr6g0298871 RchiOBHm_Chr6g0298891 RchiOBHm_Chr6g0298901 RchiOBHm_Chr6g0298911 RchiOBHm_Chr7g0201801 RchiOBHm_Chr7g0211531 RchiOBHm_Chr7g0214601
rosa_laevigata RLG00000002931 RLG00000003000 RLG00000003647 RLG00000011476 RLG00000011479
rosa_multiflora Rmu_sc0001030.1_g000012 Rmu_sc0001548.1_g000041 Rmu_ssc0000397.1_g000033 Rmu_ssc0000397.1_g000036 Rmu_ssc0000397.1_g000037 Rmu_ssc0000397.1_g000039 Rmu_ssc0000397.1_g000048
rosa_roxburghii Rroxscaffold_1G00003830 Rroxscaffold_3G00247810 Rroxscaffold_3G00255060 Rroxscaffold_7G00169220 Rroxscaffold_7G00169230 Rroxscaffold_7G00169280
rosa_rugosa Rorug02G0223600 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283200 Rorug06G0283300 Rorug06G0283400 Rorug06G0283500 Rorug07G0066000
rosa_samantha Rh2AG511300 Rh2CG235100 Rh2CG496400 Rh2DG330900 Rh6BG403100 Rh6BG403300 Rh6BG403400 Rh6BG403500 Rh6CG408700 Rh6CG408900 Rh6CG409000 Rh6CG409200 Rh6DG395800 Rh6DG395900 Rh6DG396000 Rh6DG396200 Rh7AG193400 Rh7BG195100 Rh7BG436600 Rh7DG199300 Rh7DG265500
rosa_wichuraiana Rw2G016900 Rw6G034570 Rw6G034580 Rw6G034590 Rw6G034600 Rw7G016900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1772
Acc36I ACCTGC 1 cut(s) 1734
AccB7I CCANNNNNTGG 1 cut(s) 1850
AccBSI CCGCTC 1 cut(s) 211
AccI GTMKAC 3 cut(s) 127, 801, 1518
AccIII TCCGGA 1 cut(s) 518
AciI CCGC 7 cut(s) 211, 268, 310, 2613, 2809, 3498, 3870
AclI AACGTT 1 cut(s) 3906
AclWI GGATC 8 cut(s) 183, 912, 1835, 1972, 2547, 3703, 4140, 4153
AcoI YGGCCR 3 cut(s) 931, 1812, 3843
AcsI RAATTY 7 cut(s) 199, 245, 290, 1042, 1331, 3337, 3939
AcuI CTGAAG 4 cut(s) 324, 2249, 2576, 2841
AdeI CACNNNGTG 1 cut(s) 1162
AfaI GTAC 7 cut(s) 621, 1205, 1442, 1878, 2198, 3653, 3995
AfiI CCNNNNNNNGG 7 cut(s) 518, 900, 1850, 2694, 2889, 3011, 3605
AflIII ACRYGT 2 cut(s) 2119, 2767
AgeI ACCGGT 1 cut(s) 2660
AhlI ACTAGT 1 cut(s) 1879
AjiI CACGTC 1 cut(s) 1323
AjnI CCWGG 6 cut(s) 511, 1011, 2581, 2911, 3023, 4067
Alw21I GWGCWC 4 cut(s) 321, 985, 3696, 4140
Alw26I GTCTC 7 cut(s) 907, 2110, 2172, 2342, 2930, 3399, 3985
AlwI GGATC 8 cut(s) 183, 912, 1835, 1972, 2547, 3703, 4140, 4153
AlwNI CAGNNNCTG 2 cut(s) 788, 2272
Ama87I CYCGRG 1 cut(s) 2169
Aor13HI TCCGGA 1 cut(s) 518
ApoI RAATTY 7 cut(s) 199, 245, 290, 1042, 1331, 3337, 3939
ArsI GACNNNNNNTTYG 2 cut(s) 864, 896
AseI ATTAAT 2 cut(s) 387, 3315
AsiGI ACCGGT 1 cut(s) 2660
Asp700I GAANNNNTTC 1 cut(s) 2377
AspLEI GCGC 1 cut(s) 2943
AspS9I GGNCC 9 cut(s) 265, 506, 722, 971, 1172, 1723, 2587, 3176, 3572
AsuC2I CCSGG 2 cut(s) 1595, 3977
AsuHPI GGTGA 6 cut(s) 940, 1388, 1810, 2049, 2409, 3811
AvaI CYCGRG 1 cut(s) 2169
AvaII GGWCC 5 cut(s) 265, 722, 1172, 1723, 2587
AxyI CCTNAGG 1 cut(s) 2292
BaeGI GKGCMC 2 cut(s) 1158, 3977
BalI TGGCCA 3 cut(s) 933, 1814, 3845
BamHI GGATCC 1 cut(s) 4145
BanII GRGCYC 3 cut(s) 405, 3006, 3696
BarI GAAGNNNNNNTAC 4 cut(s) 1196, 1228, 3236, 3268
BauI CACGAG 3 cut(s) 48, 1176, 2013
BbsI GAAGAC 3 cut(s) 36, 63, 4098
Bbv12I GWGCWC 4 cut(s) 321, 985, 3696, 4140
BbvCI CCTCAGC 1 cut(s) 228
BceAI ACGGC 3 cut(s) 1459, 3848, 3900
BciT130I CCWGG 6 cut(s) 513, 1013, 2583, 2913, 3025, 4069
BciVI GTATCC 6 cut(s) 10, 1591, 1661, 2709, 3061, 3203
BclI TGATCA 1 cut(s) 136
BcnI CCSGG 2 cut(s) 1595, 3977
BcoDI GTCTC 7 cut(s) 907, 2110, 2172, 2342, 2930, 3399, 3985
BcuI ACTAGT 1 cut(s) 1879
BfaI CTAG 9 cut(s) 188, 429, 692, 1407, 1482, 1880, 2405, 2546, 3308
BfmI CTRYAG 4 cut(s) 1097, 1338, 3288, 3873
BfuAI ACCTGC 1 cut(s) 1734
BfuI GTATCC 6 cut(s) 10, 1591, 1661, 2709, 3061, 3203
BglI GCCNNNNNGGC 2 cut(s) 513, 3183
BglII AGATCT 3 cut(s) 565, 575, 3523
Bme1390I CCNGG 8 cut(s) 513, 1013, 1595, 2583, 2913, 3025, 3977, 4069
Bme18I GGWCC 5 cut(s) 265, 722, 1172, 1723, 2587
BmeT110I CYCGRG 1 cut(s) 2169
BmgBI CACGTC 1 cut(s) 1323
BmgT120I GGNCC 9 cut(s) 265, 506, 722, 971, 1172, 1723, 2587, 3176, 3572
BmiI GGNNCC 7 cut(s) 266, 517, 1724, 2277, 2588, 2807, 4147
BmrFI CCNGG 8 cut(s) 513, 1013, 1595, 2583, 2913, 3025, 3977, 4069
BmrI ACTGGG 1 cut(s) 3442
BmsI GCATC 5 cut(s) 1430, 1578, 3010, 3112, 3126
BmuI ACTGGG 1 cut(s) 3442
BpiI GAAGAC 3 cut(s) 36, 63, 4098
BplI GAGNNNNNCTC 4 cut(s) 673, 705, 2993, 3025
BpmI CTGGAG 4 cut(s) 495, 2364, 2934, 3508
Bpu10I CCTNAGC 2 cut(s) 228, 260
BpuEI CTTGAG 3 cut(s) 845, 1211, 3279
BpuMI CCSGG 2 cut(s) 1595, 3977
BsaBI GATNNNNATC 2 cut(s) 789, 1814
BsaI GGTCTC 3 cut(s) 2342, 2930, 3985
BsaJI CCNNGG 7 cut(s) 894, 1326, 2582, 2862, 3024, 3366, 3882
BsaWI WCCGGW 4 cut(s) 518, 667, 2660, 3071
BsaXI ACNNNNNCTCC 2 cut(s) 2142, 2172
Bsc4I CCNNNNNNNGG 7 cut(s) 518, 900, 1850, 2694, 2889, 3011, 3605
Bse118I RCCGGY 1 cut(s) 2660
Bse1I ACTGG 9 cut(s) 1012, 2036, 2204, 2347, 2640, 3448, 3491, 3599, 3971
Bse21I CCTNAGG 1 cut(s) 2292
Bse3DI GCAATG 5 cut(s) 530, 1343, 2088, 3543, 3858
Bse8I GATNNNNATC 2 cut(s) 789, 1814
BseAI TCCGGA 1 cut(s) 518
BseBI CCWGG 6 cut(s) 513, 1013, 2583, 2913, 3025, 4069
BseDI CCNNGG 7 cut(s) 894, 1326, 2582, 2862, 3024, 3366, 3882
BseGI GGATG 9 cut(s) 612, 616, 1000, 1815, 2644, 2981, 3004, 3093, 3276
BseJI GATNNNNATC 2 cut(s) 789, 1814
BseLI CCNNNNNNNGG 7 cut(s) 518, 900, 1850, 2694, 2889, 3011, 3605
BseMI GCAATG 5 cut(s) 530, 1343, 2088, 3543, 3858
BseNI ACTGG 9 cut(s) 1012, 2036, 2204, 2347, 2640, 3448, 3491, 3599, 3971
BseRI GAGGAG 2 cut(s) 1133, 2033
BseSI GKGCMC 2 cut(s) 1158, 3977
BsgI GTGCAG 4 cut(s) 906, 3073, 3527, 3614
BshTI ACCGGT 1 cut(s) 2660
BsiHKAI GWGCWC 4 cut(s) 321, 985, 3696, 4140
BsiHKCI CYCGRG 1 cut(s) 2169
BsiSI CCGG 8 cut(s) 519, 668, 1594, 2661, 3072, 3645, 3977, 4044
BslFI GGGAC 5 cut(s) 278, 885, 1736, 2412, 2800
BslI CCNNNNNNNGG 7 cut(s) 518, 900, 1850, 2694, 2889, 3011, 3605
BsmAI GTCTC 7 cut(s) 907, 2110, 2172, 2342, 2930, 3399, 3985
BsmBI CGTCTC 1 cut(s) 907
BsmFI GGGAC 5 cut(s) 278, 885, 1736, 2412, 2800
BsmI GAATGC 2 cut(s) 2083, 3887
Bso31I GGTCTC 3 cut(s) 2342, 2930, 3985
BsoBI CYCGRG 1 cut(s) 2169
Bsp1286I GDGCHC 8 cut(s) 321, 405, 985, 1158, 3006, 3696, 3977, 4140
Bsp13I TCCGGA 1 cut(s) 518
Bsp19I CCATGG 2 cut(s) 894, 2862
BspACI CCGC 7 cut(s) 211, 268, 310, 2613, 2809, 3498, 3870
BspEI TCCGGA 1 cut(s) 518
BspHI TCATGA 2 cut(s) 1497, 1692
BspLI GGNNCC 7 cut(s) 266, 517, 1724, 2277, 2588, 2807, 4147
BspMAI CTGCAG 2 cut(s) 1101, 3292
BspMI ACCTGC 1 cut(s) 1734
BspPI GGATC 8 cut(s) 183, 912, 1835, 1972, 2547, 3703, 4140, 4153
BspQI GCTCTTC 1 cut(s) 1736
BspTNI GGTCTC 3 cut(s) 2342, 2930, 3985
BsrBI CCGCTC 1 cut(s) 211
BsrDI GCAATG 5 cut(s) 530, 1343, 2088, 3543, 3858
BsrFI RCCGGY 1 cut(s) 2660
BsrI ACTGG 9 cut(s) 1012, 2036, 2204, 2347, 2640, 3448, 3491, 3599, 3971
BssAI RCCGGY 1 cut(s) 2660
BssECI CCNNGG 7 cut(s) 894, 1326, 2582, 2862, 3024, 3366, 3882
BssNAI GTATAC 1 cut(s) 128
BssSI CACGAG 3 cut(s) 48, 1176, 2013
BssT1I CCWWGG 4 cut(s) 894, 1326, 2862, 3366
Bst1107I GTATAC 1 cut(s) 128
Bst2BI CACGAG 3 cut(s) 48, 1176, 2013
Bst2UI CCWGG 6 cut(s) 513, 1013, 2583, 2913, 3025, 4069
Bst6I CTCTTC 5 cut(s) 577, 751, 1496, 1736, 3569
BstAPI GCANNNNNTGC 2 cut(s) 3459, 3542
BstC8I GCNNGC 5 cut(s) 975, 1104, 1386, 1865, 3460
BstDSI CCRYGG 3 cut(s) 894, 2862, 3882
BstENI CCTNNNNNAGG 1 cut(s) 2887
BstF5I GGATG 9 cut(s) 612, 616, 1000, 1815, 2644, 2981, 3004, 3093, 3276
BstHHI GCGC 1 cut(s) 2943
BstMAI GTCTC 7 cut(s) 907, 2110, 2172, 2342, 2930, 3399, 3985
BstNI CCWGG 6 cut(s) 513, 1013, 2583, 2913, 3025, 4069
BstNSI RCATGY 3 cut(s) 2123, 2506, 2771
BstSCI CCNGG 8 cut(s) 511, 1011, 1593, 2581, 2911, 3023, 3975, 4067
BstSFI CTRYAG 4 cut(s) 1097, 1338, 3288, 3873
BstSLI GKGCMC 2 cut(s) 1158, 3977
BstV2I GAAGAC 3 cut(s) 36, 63, 4098
BstX2I RGATCY 6 cut(s) 175, 565, 575, 1964, 3523, 4145
BstXI CCANNNNNNTGG 1 cut(s) 1810
BstYI RGATCY 6 cut(s) 175, 565, 575, 1964, 3523, 4145
BstZ17I GTATAC 1 cut(s) 128
Bsu36I CCTNAGG 1 cut(s) 2292
BsuI GTATCC 6 cut(s) 10, 1591, 1661, 2709, 3061, 3203
BtgI CCRYGG 3 cut(s) 894, 2862, 3882
BtrI CACGTC 1 cut(s) 1323
BtsCI GGATG 9 cut(s) 612, 616, 1000, 1815, 2644, 2981, 3004, 3093, 3276
BtsI GCAGTG 2 cut(s) 494, 2328
BtsIMutI CAGTG 9 cut(s) 494, 837, 1770, 2328, 3057, 3069, 3455, 3597, 4140
BveI ACCTGC 1 cut(s) 1734
Cac8I GCNNGC 5 cut(s) 975, 1104, 1386, 1865, 3460
CaiI CAGNNNCTG 2 cut(s) 788, 2272
CciI TCATGA 2 cut(s) 1497, 1692
CfoI GCGC 1 cut(s) 2943
Cfr10I RCCGGY 1 cut(s) 2660
Cfr13I GGNCC 9 cut(s) 265, 506, 722, 971, 1172, 1723, 2587, 3176, 3572
CseI GACGC 1 cut(s) 4067
Csp6I GTAC 7 cut(s) 620, 1204, 1441, 1877, 2197, 3652, 3994
CspAI ACCGGT 1 cut(s) 2660
CviQI GTAC 7 cut(s) 620, 1204, 1441, 1877, 2197, 3652, 3994
DraI TTTAAA 1 cut(s) 1468
DraIII CACNNNGTG 1 cut(s) 1162
EaeI YGGCCR 3 cut(s) 931, 1812, 3843
Eam1104I CTCTTC 5 cut(s) 577, 751, 1496, 1736, 3569
EarI CTCTTC 5 cut(s) 577, 751, 1496, 1736, 3569
Ecl136II GAGCTC 1 cut(s) 3694
Eco130I CCWWGG 4 cut(s) 894, 1326, 2862, 3366
Eco147I AGGCCT 2 cut(s) 2581, 3365
Eco24I GRGCYC 3 cut(s) 405, 3006, 3696
Eco31I GGTCTC 3 cut(s) 2342, 2930, 3985
Eco32I GATATC 1 cut(s) 1002
Eco47I GGWCC 5 cut(s) 265, 722, 1172, 1723, 2587
Eco53kI GAGCTC 1 cut(s) 3694
Eco57I CTGAAG 4 cut(s) 324, 2249, 2576, 2841
Eco81I CCTNAGG 1 cut(s) 2292
Eco88I CYCGRG 1 cut(s) 2169
EcoICRI GAGCTC 1 cut(s) 3694
EcoNI CCTNNNNNAGG 1 cut(s) 2887
EcoO109I RGGNCCY 4 cut(s) 506, 1172, 1723, 2587
EcoRI GAATTC 2 cut(s) 3337, 3939
EcoRII CCWGG 6 cut(s) 511, 1011, 2581, 2911, 3023, 4067
EcoRV GATATC 1 cut(s) 1002
EcoT14I CCWWGG 4 cut(s) 894, 1326, 2862, 3366
EcoT38I GRGCYC 3 cut(s) 405, 3006, 3696
ErhI CCWWGG 4 cut(s) 894, 1326, 2862, 3366
Esp3I CGTCTC 1 cut(s) 907
FaqI GGGAC 5 cut(s) 278, 885, 1736, 2412, 2800
FauI CCCGC 1 cut(s) 3505
FauNDI CATATG 2 cut(s) 3502, 3667
FbaI TGATCA 1 cut(s) 136
FblI GTMKAC 3 cut(s) 127, 801, 1518
FokI GGATG 9 cut(s) 619, 623, 1007, 1822, 2651, 2968, 3011, 3080, 3283
FriOI GRGCYC 3 cut(s) 405, 3006, 3696
FspBI CTAG 9 cut(s) 188, 429, 692, 1407, 1482, 1880, 2405, 2546, 3308
GlaI GCGC 1 cut(s) 2942
GsuI CTGGAG 4 cut(s) 495, 2364, 2934, 3508
HapII CCGG 8 cut(s) 519, 668, 1594, 2661, 3072, 3645, 3977, 4044
HgaI GACGC 1 cut(s) 4067
HhaI GCGC 1 cut(s) 2943
Hin6I GCGC 1 cut(s) 2941
HinP1I GCGC 1 cut(s) 2941
HincII GTYRAC 2 cut(s) 675, 2500
HindII GTYRAC 2 cut(s) 675, 2500
HindIII AAGCTT 4 cut(s) 30, 339, 1470, 3456
HpaII CCGG 8 cut(s) 519, 668, 1594, 2661, 3072, 3645, 3977, 4044
HphI GGTGA 6 cut(s) 940, 1388, 1810, 2049, 2409, 3811
Hpy166II GTNNAC 9 cut(s) 128, 675, 802, 1519, 2478, 2500, 3762, 4010, 4057
Hpy8I GTNNAC 9 cut(s) 128, 675, 802, 1519, 2478, 2500, 3762, 4010, 4057
HpyCH4IV ACGT 6 cut(s) 727, 1322, 2066, 2474, 3171, 3906
HpySE526I ACGT 6 cut(s) 727, 1322, 2066, 2474, 3171, 3906
HspAI GCGC 1 cut(s) 2941
Kpn2I TCCGGA 1 cut(s) 518
Ksp22I TGATCA 1 cut(s) 136
LguI GCTCTTC 1 cut(s) 1736
LmnI GCTCC 7 cut(s) 208, 521, 1628, 2811, 3144, 3699, 3987
LweI GCATC 5 cut(s) 1430, 1578, 3010, 3112, 3126
MaeI CTAG 9 cut(s) 188, 429, 692, 1407, 1482, 1880, 2405, 2546, 3308
MaeII ACGT 6 cut(s) 727, 1322, 2066, 2474, 3171, 3906
MaeIII GTNAC 9 cut(s) 586, 1084, 1258, 1318, 1558, 2415, 2770, 3251, 3280
MbiI CCGCTC 1 cut(s) 211
MfeI CAATTG 2 cut(s) 2955, 3036
MflI RGATCY 6 cut(s) 175, 565, 575, 1964, 3523, 4145
MhlI GDGCHC 8 cut(s) 321, 405, 985, 1158, 3006, 3696, 3977, 4140
MlsI TGGCCA 3 cut(s) 933, 1814, 3845
MluNI TGGCCA 3 cut(s) 933, 1814, 3845
MlyI GAGTC 4 cut(s) 939, 1245, 1681, 2792
MmeI TCCRAC 7 cut(s) 186, 262, 320, 700, 2093, 2884, 3255
Mox20I TGGCCA 3 cut(s) 933, 1814, 3845
MroI TCCGGA 1 cut(s) 518
MroXI GAANNNNTTC 1 cut(s) 2377
MscI TGGCCA 3 cut(s) 933, 1814, 3845
MseI TTAA 7 cut(s) 323, 387, 570, 1467, 3315, 3531, 4176
MslI CAYNNNNRTG 6 cut(s) 324, 389, 1622, 1646, 1808, 3725
Msp20I TGGCCA 3 cut(s) 933, 1814, 3845
MspA1I CMGCKG 1 cut(s) 3872
MspI CCGG 8 cut(s) 519, 668, 1594, 2661, 3072, 3645, 3977, 4044
MspR9I CCNGG 8 cut(s) 513, 1013, 1595, 2583, 2913, 3025, 3977, 4069
MunI CAATTG 2 cut(s) 2955, 3036
Mva1269I GAATGC 2 cut(s) 2083, 3887
MvaI CCWGG 6 cut(s) 513, 1013, 2583, 2913, 3025, 4069
NciI CCSGG 2 cut(s) 1595, 3977
NcoI CCATGG 2 cut(s) 894, 2862
NdeI CATATG 2 cut(s) 3502, 3667
NlaIV GGNNCC 7 cut(s) 266, 517, 1724, 2277, 2588, 2807, 4147
NmuCI GTSAC 4 cut(s) 1258, 1318, 1558, 2415
NspI RCATGY 3 cut(s) 2123, 2506, 2771
PaeR7I CTCGAG 1 cut(s) 2169
PagI TCATGA 2 cut(s) 1497, 1692
PceI AGGCCT 2 cut(s) 2581, 3365
PciI ACATGT 2 cut(s) 2119, 2767
PciSI GCTCTTC 1 cut(s) 1736
PcsI WCGNNNNNNNCGW 1 cut(s) 2072
PctI GAATGC 2 cut(s) 2083, 3887
PdmI GAANNNNTTC 1 cut(s) 2377
PfeI GAWTC 7 cut(s) 68, 785, 1776, 2141, 2446, 2828, 4040
PflMI CCANNNNNTGG 1 cut(s) 1850
PinAI ACCGGT 1 cut(s) 2660
PleI GAGTC 4 cut(s) 939, 1244, 1681, 2791
PpsI GAGTC 4 cut(s) 939, 1244, 1681, 2791
PpuMI RGGWCCY 3 cut(s) 1172, 1723, 2587
PscI ACATGT 2 cut(s) 2119, 2767
PshBI ATTAAT 2 cut(s) 387, 3315
PsiI TTATAA 1 cut(s) 1772
Psp124BI GAGCTC 1 cut(s) 3696
Psp1406I AACGTT 1 cut(s) 3906
Psp5II RGGWCCY 3 cut(s) 1172, 1723, 2587
Psp6I CCWGG 6 cut(s) 511, 1011, 2581, 2911, 3023, 4067
PspGI CCWGG 6 cut(s) 511, 1011, 2581, 2911, 3023, 4067
PspN4I GGNNCC 7 cut(s) 266, 517, 1724, 2277, 2588, 2807, 4147
PspPI GGNCC 9 cut(s) 265, 506, 722, 971, 1172, 1723, 2587, 3176, 3572
PspPPI RGGWCCY 3 cut(s) 1172, 1723, 2587
PstI CTGCAG 2 cut(s) 1101, 3292
PstNI CAGNNNCTG 2 cut(s) 788, 2272
PsuI RGATCY 6 cut(s) 175, 565, 575, 1964, 3523, 4145
RsaI GTAC 7 cut(s) 621, 1205, 1442, 1878, 2198, 3653, 3995
RsaNI GTAC 7 cut(s) 620, 1204, 1441, 1877, 2197, 3652, 3994
RseI CAYNNNNRTG 6 cut(s) 324, 389, 1622, 1646, 1808, 3725
SacI GAGCTC 1 cut(s) 3696
SapI GCTCTTC 1 cut(s) 1736
SaqAI TTAA 7 cut(s) 323, 387, 570, 1467, 3315, 3531, 4176
Sau96I GGNCC 9 cut(s) 265, 506, 722, 971, 1172, 1723, 2587, 3176, 3572
SchI GAGTC 4 cut(s) 939, 1245, 1681, 2792
ScrFI CCNGG 8 cut(s) 513, 1013, 1595, 2583, 2913, 3025, 3977, 4069
SduI GDGCHC 8 cut(s) 321, 405, 985, 1158, 3006, 3696, 3977, 4140
SfaNI GCATC 5 cut(s) 1430, 1578, 3010, 3112, 3126
SfcI CTRYAG 4 cut(s) 1097, 1338, 3288, 3873
Sfr274I CTCGAG 1 cut(s) 2169
SinI GGWCC 5 cut(s) 265, 722, 1172, 1723, 2587
SlaI CTCGAG 1 cut(s) 2169
SmiMI CAYNNNNRTG 6 cut(s) 324, 389, 1622, 1646, 1808, 3725
SmlI CTYRAG 4 cut(s) 860, 1190, 2169, 3294
SmoI CTYRAG 4 cut(s) 860, 1190, 2169, 3294
SpeI ACTAGT 1 cut(s) 1879
SseBI AGGCCT 2 cut(s) 2581, 3365
SsiI CCGC 7 cut(s) 211, 268, 310, 2613, 2809, 3498, 3870
SspI AATATT 4 cut(s) 775, 1286, 2991, 3428
SspMI CTAG 9 cut(s) 188, 429, 692, 1407, 1482, 1880, 2405, 2546, 3308
SstI GAGCTC 1 cut(s) 3696
StuI AGGCCT 2 cut(s) 2581, 3365
StyD4I CCNGG 8 cut(s) 511, 1011, 1593, 2581, 2911, 3023, 3975, 4067
StyI CCWWGG 4 cut(s) 894, 1326, 2862, 3366
TaiI ACGT 6 cut(s) 730, 1325, 2069, 2477, 3174, 3909
TaqI TCGA 5 cut(s) 315, 1004, 2170, 2542, 2930
TatI WGTACW 3 cut(s) 1203, 1876, 3651
TfiI GAWTC 7 cut(s) 68, 785, 1776, 2141, 2446, 2828, 4040
Tru1I TTAA 7 cut(s) 323, 387, 570, 1467, 3315, 3531, 4176
Tru9I TTAA 7 cut(s) 323, 387, 570, 1467, 3315, 3531, 4176
TscAI CASTG 9 cut(s) 501, 844, 1770, 2328, 3057, 3069, 3455, 3597, 4140
TseFI GTSAC 4 cut(s) 1258, 1318, 1558, 2415
Tsp45I GTSAC 4 cut(s) 1258, 1318, 1558, 2415
TspGWI ACGGA 4 cut(s) 1594, 1920, 4011, 4138
TspRI CASTG 9 cut(s) 501, 844, 1770, 2328, 3057, 3069, 3455, 3597, 4140
Van91I CCANNNNNTGG 1 cut(s) 1850
VpaK11BI GGWCC 5 cut(s) 265, 722, 1172, 1723, 2587
VspI ATTAAT 2 cut(s) 387, 3315
XagI CCTNNNNNAGG 1 cut(s) 2887
XapI RAATTY 7 cut(s) 199, 245, 290, 1042, 1331, 3337, 3939
XbaI TCTAGA 3 cut(s) 1406, 1481, 2404
XceI RCATGY 3 cut(s) 2123, 2506, 2771
XcmI CCANNNNNNNNNTGG 6 cut(s) 535, 1789, 2190, 2314, 2869, 2992
XhoI CTCGAG 1 cut(s) 2169
XmiI GTMKAC 3 cut(s) 127, 801, 1518
XmnI GAANNNNTTC 1 cut(s) 2377
XspI CTAG 9 cut(s) 188, 429, 692, 1407, 1482, 1880, 2405, 2546, 3308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.