Rw6G034600
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000493
Physical Location & Seq
Forward (+)
59226359 .. 59232825
6467 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G034600.1

Sequence Viewer

Length: 4227 bp
ATGGAAGTGTTTTCAAGGTCTTTTCGGGGTGAAGACGATGAAGAAGCTCTCAAATGGGCTGCAATTGAGAGGTTGCCTACTGGTTTGCGTATCGGGAGAGGCTTGTTCATTGATGGTGAGGGCCAAGCTAGGGAGGTTGATGTCGAAAACCTTGGTTTACTTGAGAGGAAGACTTTGTTGGAGAGGCTGGTGAACAATGCGGAGAAAGATAATGAGAAGTTCTTGTTAAAACTCAAGAACCGAATTGACAGAGTTGGACTTGATATGCCAACAATTGAAGTTCGGTTTGAGCATCTTACTGTGGAGGCAAAAGTTTATTTGGGAAGCAGAGCTCTGCCTACTTTACTCAACTTTGCCATCAATATGTTACAGGGATGGTTACATTATCTTCACATTTTTCCAAGTAGAAAGGCATCCTTAACAATCCTTCAAAATGTTAGTGGCATTATCAAGCCCCAAAGAATGACTTTGCTTTTAGGTCCCCCAAGTTCCGGAAAAACCACATTACTATTGTCTCTTGCAGGAAGACTTGATAAAGATCTAAGATCTTCGGGAGAAGTTACGTATAATGGACACGGTATGGCGGAATTTGTAGCTCAGAGAACATCAGCTTATATAAGTCAAAATGATCTTCACATAGGAGAAATGACAGTGAAGGAGACACTGGCTTTCTCAGCTAGATGTCAAGGAGTTGGATCAAATTACGACATGTTGGCAGAATTGTCTAGAAGAGAGAAGGAGGAAAATATCAAACCTGATCCCGACATTGATATCTACCAAAAGGCAGCAGCACTAGAAGGGCAGGAGAGTAGTGTAGTTGCTGATTACATTCTCAAGATTTTGGGACTTGAAGTTTGTGCCAATACTATGGTGGGGGATGAAATGGTAAGAGGCATCTCGGGTGGAGAAAGAAAGCGAGTCACAATAGGGGAGATGCTTGTTGGACCTGTAAGAGCCCTCTTCATGGATGAGATATCAACTGGCTTGGACAGTTCAACGACTTTCCAGATAGTAAACTCACTCAGGCAATCCATCCATATTCTTAGGGGAACTGCAGTTGTTTCCCTCCTACAGCCAGCCCCCGAAACTTATAGCCTATTCGATGATATAATTCTCCTATCAGATGGCCAAATAGTGTATCAGGGTCCACGTGAAAGTGTGCTCGACTTTTTTGAATTCATGGGGTTCAAATGTCCCGAGAGGAAAGGAGTTGCTGACTTCTTGCAAGAGGTGACATCCAGGAAAGATCAAGAGCAGTATTGGGCACGTTTACATGAGCCTTACAGATTTGTTACTGTCACTGAGTTTGCTGACGCATTCCAGGCATTTCATATTGGCCAGAGACTTGGTGATGAGCTTGCTAGTCCCTTCGACAGGTCTAAAAGCCACCGTGCTGCTTTAACAGCTAAGAAATATGGTGTTAACACGAAGGAGTTGTTCAGAGCTTGTATTTCTAGAGAGTTTTTGCTCATGAAGAGGAATTCATTTGCCTACATTTTCAAACTGGCTCTACTTATCATCTTAGCTTCTATAACAACAACAATATTTCTAAGAGTTGAGATGCATAAAGGCACTGTGTTGGATGGGGGAATCTATCTGGGTGCTTTGTTCTTTGCAGTCATTGTAGCGATGTTTAATGGAATATCAGAGCTGAACATGGCCATTATGAAACTTCCCGTCTTTTACAAGCAAAGAGACCTCCTCTTCTATCCTGCATGGGCATACTCAATACCTGGATGGATACTCAAGATCCCAATCACTTTAGTAGAGAGTTTCATTTGGGTGATCATTACTTATTATGTCATAGGATTTGATCCACATGTTGAAAGGTTCCTCAAGCAGTACATGTTACTGGTGTGCATAAACCAGATGGCGTCCGGTTTGTTCAGGTGCATGGCAGCAATAGGAAGGAGTGTAATAGTTGCGAGCACATTTGGAACCTTTGCATTACTTGTAATCTTGGTTTTGGGTGGATTTGTCTTATCTCGAGAGGCTGTACCAAAATGGTGGTTGTGGGGGTACTGGGTCTCACCGTTGATGTATGGACAAAATGCCATAGCTGTGAATGAATTCCTTGGAAAGAGTTGGAGTCATGTTCCACATGGTTCTACAGAATCCTTGGGAGTTATGGTCCTGAAGTCTCACGGCATTTTCCCAGAAGCACATTGGTATTGGATTGGAGTAGCAGCTTTAGTTGGATACACTTTTCTGTTCAACATCATCATTACTTTCGCTCTACAATACCTGGATCCATTTGGGAAGCCTCAGGCAGTACTGTCCAAAGAAAGCATAGCGGAGAGAAATGGTAATGGAACTGGAGAGTTCATTGAGCTATTATCAAGAGGAAATAGATATTCTGGTGGAGGAAGAAGTGATATTGCTAATGGGAAAAGGAAGCGAGGAATGGTTCTTCCTTTTCAACCCCTTTCCCTTGCTTTCGATGAGATCAGATATGCCATAGACATGCCACAGGAAATGAAAGCACAGGGTGTTAAGGAGGATAGGCTGGAACTGTTGAAAGGTCTAAATGGTGCTTTTAGACCAGGAGTCTTAACAGCCCTGATGGGTGTTAGTGGTGCAGGCAAGACCACTTTAATGGATGTCTTGGCCGGAAGGAAAACTGGTGGATACATTGAGGGAAGCATTGTGATATCTGGGTACTGTAAAAGGCAAGAAACATTTGCTCGCATATCAGGTTATTGTGAGCAAACTGATATCCACTCTCCTCATGTTACAGTCTATGAATCCCTGCTGTTTTCTGCTTGGCTTCGACTACTGCCTGAGGTTGATTCTGCAACTAGGAAGATGTTTATCGAGGAAGTTATGGAGCTTGTGGAGCTGACTTCACTTAGGGGAGCACTTGTGGGATTACCAGGTGTAACTGGCCTTTCAACCGAGCAGCGTAAAAGACTGACTATTGCCGTTGAGCTTGTTGCAAATCCATCTATAATTTTCATGGATGAGCCAACATCTGGCCTTGATGCCAGGGCAGCAGCAATAGTGATGAGAACAGTTAGGAACACAGTGGACACAGGCCGAACTGTGGTTTGCACTATCCACCAGCCTAGCATAGATATATTCGAAGCTTTTGATGAGCTTCTACTTCTCAAATTGGGAGGTGAGCCAATATACGCCGGTCCATTAGGACTCCATTCCTCCCTTCTGATCAAGTACTTTGAGGGAATTAATGGCGTTCCTAAAGTTAAAGATGGATATAACCCTGCAACCTGGATGTTGGAGATTACTTCAGCCGCACAAGAGGAAGCTCTTGGGGTTAACTTCACAGATGTGTACAAGAAATCAGAACTATACAAGAGAACTAAAGATATGATCAAGGAATTGTCTATACCTCCCTCAGGTTCTAAGGATCTGTACTTTCCTACTCGCTACTCGCAATCTTTTCACACCCAATGCATGGCTTGCCTATGGAAACAACACTGGTCGTACTGGCGAAACCCATCATATAGTGCAGTTAGACTCTTATTCACAACATTCATCGCGTTGTTGTTTGGTATGATATTTTGGGATCTCGGGTCAAAAAGGAGCAAGCAGCAAGACATCTTCAATGCAATGGGCTCTATATATTCTGCTGTTATTTTTCTCGGGGTCCAGAATGCTGCCTCAGTGCAGCCAATTGTGGCTGTTGAGAGAACAGTGTTTTACAGAGAAAGAGCAGCTGGTATGTACTCAGCTTTGCCTTACGCATTTGGACAGATGATGATTGAGGTTCCCTACATTTTCATTCAGACAATTATTTATGGGGTTATAGTATATGCAATGATGGGATTCGATTGGATAGTTTCCAAGTTCCTTTGGTACCTTTTCTTCATGTACTTCACCTTATTATACTTCGCATTCTATGGCATGATGACCATGGCTCTAACTCCCAACCACAACATTGCTGCTATAACTTCATCTTCCTTCTATGCAATATGGAACCTTTTCTCAGGATTTGTCATTCCTCCATCGAGAATACCTATATGGTGGAAATGGTACTATTGGATTTGCCCTGTTGCTTGGACCTTGTATGGAATGGCCGCTTCGCAGTTTGGAGACATAAAGCAGAGACTCGAGACTGGCGAAACTGTGGAAGATTTTTTGACAAGCTACTTTGGATACAGGCACGACTTTCTGGGTATCGTTGCAGTGGTGGTTGTCGGGTTTTCATTCCTGTTAGGCTTTATCTTTGCTTATTCCATTCGAGCTTTTAACTTCCAAAAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1408

Amino Acids

158.6

Weight (kDa)

8.01

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 66 - 118 4.7e-08 ABC-transporter N-terminal
ABC_tran PF00005 143 - 324 9.1e-16 ABC transporter
ABC2_membrane PF01061 479 - 691 1e-48 ABC-2 type transporter
PDR_assoc PF08370 696 - 758 7e-28 Plant PDR ABC transporter associated
ABC_tran PF00005 839 - 991 9.3e-19 ABC transporter
ABC2_membrane PF01061 1136 - 1350 7.2e-58 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000493)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29820 FvH4_2g29820 FvH4_2g29822 FvH4_2g29824 FvH4_2g29824 FvH4_2g29824 FvH4_2g29840 FvH4_2g29840 FvH4_2g29840 FvH4_5g00550 FvH4_5g00550
malus_domestica MD02G1032700.v1.1 MD08G1140900.v1.1 MD08G1141400.v1.1 MD15G1117800.v1.1 MD15G1117900.v1.1
prunus_persica Prupe.1G471200_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471400_v2.0.a1 Prupe.7G242900_v2.0.a1
pyrus_communis pycom02g02690 pycom08g11890
rosa_chinensis RchiOBHm_Chr2g0111741 RchiOBHm_Chr2g0145991 RchiOBHm_Chr6g0298851 RchiOBHm_Chr6g0298871 RchiOBHm_Chr6g0298891 RchiOBHm_Chr6g0298901 RchiOBHm_Chr6g0298911 RchiOBHm_Chr7g0201801 RchiOBHm_Chr7g0211531 RchiOBHm_Chr7g0214601
rosa_laevigata RLG00000002931 RLG00000003000 RLG00000003647 RLG00000011476 RLG00000011479
rosa_multiflora Rmu_sc0001030.1_g000012 Rmu_sc0001548.1_g000041 Rmu_ssc0000397.1_g000033 Rmu_ssc0000397.1_g000036 Rmu_ssc0000397.1_g000037 Rmu_ssc0000397.1_g000039 Rmu_ssc0000397.1_g000048
rosa_roxburghii Rroxscaffold_1G00003830 Rroxscaffold_3G00247810 Rroxscaffold_3G00255060 Rroxscaffold_7G00169220 Rroxscaffold_7G00169230 Rroxscaffold_7G00169280
rosa_rugosa Rorug02G0223600 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283200 Rorug06G0283300 Rorug06G0283400 Rorug06G0283500 Rorug07G0066000
rosa_samantha Rh2AG511300 Rh2CG235100 Rh2CG496400 Rh2DG330900 Rh6BG403100 Rh6BG403300 Rh6BG403400 Rh6BG403500 Rh6CG408700 Rh6CG408900 Rh6CG409000 Rh6CG409200 Rh6DG395800 Rh6DG395900 Rh6DG396000 Rh6DG396200 Rh7AG193400 Rh7BG195100 Rh7BG436600 Rh7DG199300 Rh7DG265500
rosa_wichuraiana Rw2G016900 Rw6G034570 Rw6G034580 Rw6G034590 Rw6G034600 Rw7G016900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 3816
AccB1I GGYRCC 1 cut(s) 3816
AccB7I CCANNNNNTGG 2 cut(s) 2972, 3415
AccII CGCG 1 cut(s) 3500
AccIII TCCGGA 1 cut(s) 491
AciI CCGC 5 cut(s) 200, 584, 2294, 3252, 4038
AclWI GGATC 8 cut(s) 703, 752, 1744, 1808, 2243, 2256, 3375, 3534
AcoI YGGCCR 5 cut(s) 1126, 1336, 1659, 2607, 4035
AcsI RAATTY 4 cut(s) 587, 1175, 1480, 2069
AcuI CTGAAG 2 cut(s) 2156, 3231
AcvI CACGTG 1 cut(s) 1151
AcyI GRCGYC 1 cut(s) 1874
AdeI CACNNNGTG 1 cut(s) 2489
AfiI CCNNNNNNNGG 8 cut(s) 130, 491, 964, 1374, 2972, 3043, 3356, 3415
AflIII ACRYGT 3 cut(s) 708, 1819, 1845
AhdI GACNNNNNGTC 1 cut(s) 2546
AjnI CCWGG 8 cut(s) 1238, 1320, 1732, 2244, 2542, 2872, 2984, 3227
AjuI GAANNNNNNNTTGG 2 cut(s) 161, 193
AleI CACNNNNGTG 1 cut(s) 3287
Alw21I GWGCWC 4 cut(s) 334, 1164, 1931, 2860
Alw26I GTCTC 9 cut(s) 519, 653, 1336, 1689, 2032, 2145, 4047, 4060, 4067
AlwI GGATC 8 cut(s) 703, 752, 1744, 1808, 2243, 2256, 3375, 3534
Ama87I CYCGRG 6 cut(s) 898, 1196, 1986, 3530, 3602, 4070
Aor13HI TCCGGA 1 cut(s) 491
AoxI GGCC 9 cut(s) 121, 1126, 1336, 1659, 2607, 2884, 2974, 3034, 4035
ApoI RAATTY 4 cut(s) 587, 1175, 1480, 2069
ArsI GACNNNNNNTTYG 2 cut(s) 837, 869
AseI ATTAAT 1 cut(s) 3186
Asp700I GAANNNNTTC 2 cut(s) 2069, 3941
Asp718I GGTACC 1 cut(s) 3816
AspS9I GGNCC 8 cut(s) 121, 479, 944, 1145, 2131, 3137, 3607, 4020
AsuHPI GGTGA 9 cut(s) 41, 128, 202, 1243, 1361, 1795, 2022, 3131, 3829
AsuII TTCGAA 1 cut(s) 3081
AvaI CYCGRG 6 cut(s) 898, 1196, 1986, 3530, 3602, 4070
AvaII GGWCC 7 cut(s) 479, 944, 1145, 2131, 3137, 3607, 4020
AxyI CCTNAGG 3 cut(s) 2265, 2781, 3355
BaeGI GKGCMC 1 cut(s) 1267
BalI TGGCCA 3 cut(s) 1128, 1338, 1661
BamHI GGATCC 1 cut(s) 2248
BanI GGYRCC 1 cut(s) 3816
BanII GRGCYC 3 cut(s) 334, 958, 3578
BarI GAAGNNNNNNTAC 2 cut(s) 3809, 3841
BbrPI CACGTG 1 cut(s) 1151
BbsI GAAGAC 3 cut(s) 39, 176, 532
Bbv12I GWGCWC 4 cut(s) 334, 1164, 1931, 2860
BceAI ACGGC 2 cut(s) 2161, 2906
BciT130I CCWGG 8 cut(s) 1240, 1322, 1734, 2246, 2544, 2874, 2986, 3229
BciVI GTATCC 4 cut(s) 1734, 2192, 2621, 4109
BclI TGATCA 3 cut(s) 1785, 3165, 3330
BcoDI GTCTC 9 cut(s) 519, 653, 1336, 1689, 2032, 2145, 4047, 4060, 4067
BfaI CTAG 8 cut(s) 129, 678, 726, 794, 1362, 1455, 2799, 3066
BfmI CTRYAG 3 cut(s) 1053, 1070, 2109
BfuI GTATCC 4 cut(s) 1734, 2192, 2621, 4109
BglII AGATCT 2 cut(s) 538, 545
BmcAI AGTACT 2 cut(s) 2274, 3173
Bme1390I CCNGG 8 cut(s) 1240, 1322, 1734, 2246, 2544, 2874, 2986, 3229
Bme18I GGWCC 7 cut(s) 479, 944, 1145, 2131, 3137, 3607, 4020
BmeRI GACNNNNNGTC 1 cut(s) 2546
BmeT110I CYCGRG 6 cut(s) 898, 1196, 1986, 3530, 3602, 4070
BmgT120I GGNCC 8 cut(s) 121, 479, 944, 1145, 2131, 3137, 3607, 4020
BmiI GGNNCC 9 cut(s) 481, 1146, 1832, 1939, 2250, 3608, 3729, 3818, 3938
BmrFI CCNGG 8 cut(s) 1240, 1322, 1734, 2246, 2544, 2874, 2986, 3229
BmrI ACTGGG 1 cut(s) 2032
BmsI GCATC 6 cut(s) 301, 422, 903, 924, 1551, 2971
BmuI ACTGGG 1 cut(s) 2032
BpiI GAAGAC 3 cut(s) 39, 176, 532
BplI GAGNNNNNCTC 2 cut(s) 1686, 1718
BpmI CTGGAG 1 cut(s) 2337
Bpu14I TTCGAA 1 cut(s) 3081
BpuEI CTTGAG 5 cut(s) 182, 218, 818, 1730, 1820
BsaAI YACGTR 2 cut(s) 564, 1151
BsaBI GATNNNNATC 3 cut(s) 537, 1754, 2810
BsaHI GRCGYC 1 cut(s) 1874
BsaI GGTCTC 2 cut(s) 1689, 2032
BsaJI CCNNGG 5 cut(s) 151, 2074, 2118, 2985, 3873
BsaWI WCCGGW 2 cut(s) 491, 1877
BsaXI ACNNNNNCTCC 4 cut(s) 2115, 2145, 2847, 2877
Bsc4I CCNNNNNNNGG 8 cut(s) 130, 491, 964, 1374, 2972, 3043, 3356, 3415
Bse118I RCCGGY 1 cut(s) 3134
Bse21I CCTNAGG 3 cut(s) 2265, 2781, 3355
Bse3DI GCAATG 3 cut(s) 3576, 3783, 3897
Bse8I GATNNNNATC 3 cut(s) 537, 1754, 2810
BseAI TCCGGA 1 cut(s) 491
BseBI CCWGG 8 cut(s) 1240, 1322, 1734, 2246, 2544, 2874, 2986, 3229
BseDI CCNNGG 5 cut(s) 151, 2074, 2118, 2985, 3873
BseJI GATNNNNATC 3 cut(s) 537, 1754, 2810
BseLI CCNNNNNNNGG 8 cut(s) 130, 491, 964, 1374, 2972, 3043, 3356, 3415
BseMI GCAATG 3 cut(s) 3576, 3783, 3897
BseRI GAGGAG 2 cut(s) 1691, 2715
BseSI GKGCMC 1 cut(s) 1267
BsgI GTGCAG 3 cut(s) 2598, 3489, 3647
Bsh1236I CGCG 1 cut(s) 3500
BshFI GGCC 9 cut(s) 123, 1128, 1338, 1661, 2609, 2886, 2976, 3036, 4037
BshNI GGYRCC 1 cut(s) 3816
BsiHKAI GWGCWC 4 cut(s) 334, 1164, 1931, 2860
BsiHKCI CYCGRG 6 cut(s) 898, 1196, 1986, 3530, 3602, 4070
BsiSI CCGG 4 cut(s) 492, 1878, 2610, 3135
BslFI GGGAC 4 cut(s) 465, 858, 1179, 1350
BslI CCNNNNNNNGG 8 cut(s) 130, 491, 964, 1374, 2972, 3043, 3356, 3415
BsmAI GTCTC 9 cut(s) 519, 653, 1336, 1689, 2032, 2145, 4047, 4060, 4067
BsmFI GGGAC 4 cut(s) 465, 858, 1179, 1350
BsmI GAATGC 3 cut(s) 1316, 3619, 3854
BsnI GGCC 9 cut(s) 123, 1128, 1338, 1661, 2609, 2886, 2976, 3036, 4037
Bso31I GGTCTC 2 cut(s) 1689, 2032
BsoBI CYCGRG 6 cut(s) 898, 1196, 1986, 3530, 3602, 4070
Bsp119I TTCGAA 1 cut(s) 3081
Bsp1286I GDGCHC 7 cut(s) 334, 958, 1164, 1267, 1931, 2860, 3578
Bsp13I TCCGGA 1 cut(s) 491
Bsp1407I TGTACA 1 cut(s) 3291
Bsp19I CCATGG 1 cut(s) 3873
BspACI CCGC 5 cut(s) 200, 584, 2294, 3252, 4038
BspANI GGCC 9 cut(s) 123, 1128, 1338, 1661, 2609, 2886, 2976, 3036, 4037
BspEI TCCGGA 1 cut(s) 491
BspFNI CGCG 1 cut(s) 3500
BspHI TCATGA 1 cut(s) 1470
BspLI GGNNCC 9 cut(s) 481, 1146, 1832, 1939, 2250, 3608, 3729, 3818, 3938
BspMAI CTGCAG 1 cut(s) 1057
BspPI GGATC 8 cut(s) 703, 752, 1744, 1808, 2243, 2256, 3375, 3534
BspT104I TTCGAA 1 cut(s) 3081
BspT107I GGYRCC 1 cut(s) 3816
BspTNI GGTCTC 2 cut(s) 1689, 2032
BsrDI GCAATG 3 cut(s) 3576, 3783, 3897
BsrFI RCCGGY 1 cut(s) 3134
BsrGI TGTACA 1 cut(s) 3291
BssAI RCCGGY 1 cut(s) 3134
BssECI CCNNGG 5 cut(s) 151, 2074, 2118, 2985, 3873
BssNI GRCGYC 1 cut(s) 1874
BssT1I CCWWGG 4 cut(s) 151, 2074, 2118, 3873
Bst2UI CCWGG 8 cut(s) 1240, 1322, 1734, 2246, 2544, 2874, 2986, 3229
Bst6I CTCTTC 4 cut(s) 724, 965, 1469, 1709
BstACI GRCGYC 1 cut(s) 1874
BstAPI GCANNNNNTGC 1 cut(s) 3420
BstAUI TGTACA 1 cut(s) 3291
BstBAI YACGTR 2 cut(s) 564, 1151
BstBI TTCGAA 1 cut(s) 3081
BstC8I GCNNGC 7 cut(s) 1077, 1359, 1927, 2581, 2686, 3421, 3548
BstDSI CCRYGG 1 cut(s) 3873
BstENI CCTNNNNNAGG 2 cut(s) 1372, 3354
BstFNI CGCG 1 cut(s) 3500
BstMAI GTCTC 9 cut(s) 519, 653, 1336, 1689, 2032, 2145, 4047, 4060, 4067
BstMWI GCNNNNNNNGC 6 cut(s) 674, 1322, 1403, 2836, 2990, 3420
BstNI CCWGG 8 cut(s) 1240, 1322, 1734, 2246, 2544, 2874, 2986, 3229
BstNSI RCATGY 4 cut(s) 712, 1823, 1849, 2467
BstSCI CCNGG 8 cut(s) 1238, 1320, 1732, 2244, 2542, 2872, 2984, 3227
BstSFI CTRYAG 3 cut(s) 1053, 1070, 2109
BstSLI GKGCMC 1 cut(s) 1267
BstSNI TACGTA 1 cut(s) 564
BstUI CGCG 1 cut(s) 3500
BstV2I GAAGAC 3 cut(s) 39, 176, 532
BstX2I RGATCY 6 cut(s) 538, 545, 1749, 2248, 3367, 3526
BstXI CCANNNNNNTGG 4 cut(s) 868, 1346, 2007, 2596
BstYI RGATCY 6 cut(s) 538, 545, 1749, 2248, 3367, 3526
Bsu36I CCTNAGG 3 cut(s) 2265, 2781, 3355
BsuI GTATCC 4 cut(s) 1734, 2192, 2621, 4109
BsuRI GGCC 9 cut(s) 123, 1128, 1338, 1661, 2609, 2886, 2976, 3036, 4037
BtgI CCRYGG 1 cut(s) 3873
BtgZI GCGATG 2 cut(s) 1643, 3481
BtsI GCAGTG 1 cut(s) 4152
BtsIMutI CAGTG 9 cut(s) 657, 662, 1299, 1572, 3030, 3436, 3630, 3660, 4152
Cac8I GCNNGC 7 cut(s) 1077, 1359, 1927, 2581, 2686, 3421, 3548
CciI TCATGA 1 cut(s) 1470
Cfr10I RCCGGY 1 cut(s) 3134
Cfr13I GGNCC 8 cut(s) 121, 479, 944, 1145, 2131, 3137, 3607, 4020
CseI GACGC 2 cut(s) 1322, 1863
CsiI ACCWGGT 1 cut(s) 2872
DraIII CACNNNGTG 1 cut(s) 2489
DriI GACNNNNNGTC 1 cut(s) 2546
EaeI YGGCCR 5 cut(s) 1126, 1336, 1659, 2607, 4035
Eam1104I CTCTTC 4 cut(s) 724, 965, 1469, 1709
Eam1105I GACNNNNNGTC 1 cut(s) 2546
EarI CTCTTC 4 cut(s) 724, 965, 1469, 1709
EciI GGCGGA 1 cut(s) 599
Ecl136II GAGCTC 1 cut(s) 332
Eco105I TACGTA 1 cut(s) 564
Eco130I CCWWGG 4 cut(s) 151, 2074, 2118, 3873
Eco24I GRGCYC 3 cut(s) 334, 958, 3578
Eco31I GGTCTC 2 cut(s) 1689, 2032
Eco32I GATATC 4 cut(s) 772, 975, 2652, 2716
Eco47I GGWCC 7 cut(s) 479, 944, 1145, 2131, 3137, 3607, 4020
Eco53kI GAGCTC 1 cut(s) 332
Eco57I CTGAAG 2 cut(s) 2156, 3231
Eco72I CACGTG 1 cut(s) 1151
Eco81I CCTNAGG 3 cut(s) 2265, 2781, 3355
Eco88I CYCGRG 6 cut(s) 898, 1196, 1986, 3530, 3602, 4070
EcoICRI GAGCTC 1 cut(s) 332
EcoNI CCTNNNNNAGG 2 cut(s) 1372, 3354
EcoO109I RGGNCCY 1 cut(s) 479
EcoRI GAATTC 3 cut(s) 1175, 1480, 2069
EcoRII CCWGG 8 cut(s) 1238, 1320, 1732, 2244, 2542, 2872, 2984, 3227
EcoRV GATATC 4 cut(s) 772, 975, 2652, 2716
EcoT14I CCWWGG 4 cut(s) 151, 2074, 2118, 3873
EcoT22I ATGCAT 2 cut(s) 1566, 3416
EcoT38I GRGCYC 3 cut(s) 334, 958, 3578
ErhI CCWWGG 4 cut(s) 151, 2074, 2118, 3873
FalI AAGNNNNNCTT 4 cut(s) 401, 433, 451, 483
FaqI GGGAC 4 cut(s) 465, 858, 1179, 1350
FbaI TGATCA 3 cut(s) 1785, 3165, 3330
FriOI GRGCYC 3 cut(s) 334, 958, 3578
FspBI CTAG 8 cut(s) 129, 678, 726, 794, 1362, 1455, 2799, 3066
GsuI CTGGAG 1 cut(s) 2337
HaeIII GGCC 9 cut(s) 123, 1128, 1338, 1661, 2609, 2886, 2976, 3036, 4037
HapII CCGG 4 cut(s) 492, 1878, 2610, 3135
HgaI GACGC 2 cut(s) 1322, 1863
Hin1I GRCGYC 1 cut(s) 1874
HincII GTYRAC 2 cut(s) 1423, 3277
HindII GTYRAC 2 cut(s) 1423, 3277
HindIII AAGCTT 1 cut(s) 3084
HpaI GTTAAC 2 cut(s) 1423, 3277
HpaII CCGG 4 cut(s) 492, 1878, 2610, 3135
HphI GGTGA 9 cut(s) 41, 128, 202, 1243, 1361, 1795, 2022, 3131, 3829
Hpy166II GTNNAC 9 cut(s) 158, 193, 1015, 1148, 1271, 1423, 3028, 3277, 3292
Hpy188I TCNGA 8 cut(s) 600, 1123, 1442, 1648, 2450, 3165, 3304, 3747
Hpy8I GTNNAC 9 cut(s) 158, 193, 1015, 1148, 1271, 1423, 3028, 3277, 3292
HpyCH4IV ACGT 3 cut(s) 563, 1150, 1267
HpyF10VI GCNNNNNNNGC 6 cut(s) 674, 1322, 1403, 2836, 2990, 3420
HpySE526I ACGT 3 cut(s) 563, 1150, 1267
Hsp92I GRCGYC 1 cut(s) 1874
Kpn2I TCCGGA 1 cut(s) 491
KpnI GGTACC 1 cut(s) 3820
Ksp22I TGATCA 3 cut(s) 1785, 3165, 3330
KspAI GTTAAC 2 cut(s) 1423, 3277
LmnI GCTCC 4 cut(s) 2827, 2836, 2855, 3543
LweI GCATC 6 cut(s) 301, 422, 903, 924, 1551, 2971
MabI ACCWGGT 1 cut(s) 2872
MaeI CTAG 8 cut(s) 129, 678, 726, 794, 1362, 1455, 2799, 3066
MaeII ACGT 3 cut(s) 563, 1150, 1267
MfeI CAATTG 3 cut(s) 63, 273, 3633
MflI RGATCY 6 cut(s) 538, 545, 1749, 2248, 3367, 3526
MhlI GDGCHC 7 cut(s) 334, 958, 1164, 1267, 1931, 2860, 3578
MlsI TGGCCA 3 cut(s) 1128, 1338, 1661
MluNI TGGCCA 3 cut(s) 1128, 1338, 1661
MlyI GAGTC 6 cut(s) 927, 2098, 2556, 3141, 3471, 4062
MmeI TCCRAC 8 cut(s) 159, 235, 673, 922, 1560, 2066, 2176, 3216
Mox20I TGGCCA 3 cut(s) 1128, 1338, 1661
Mph1103I ATGCAT 2 cut(s) 1566, 3416
MroI TCCGGA 1 cut(s) 491
MroXI GAANNNNTTC 2 cut(s) 2069, 3941
MscI TGGCCA 3 cut(s) 1128, 1338, 1661
MslI CAYNNNNRTG 6 cut(s) 362, 1781, 2060, 2462, 2594, 3287
Msp20I TGGCCA 3 cut(s) 1128, 1338, 1661
MspA1I CMGCKG 1 cut(s) 3677
MspI CCGG 4 cut(s) 492, 1878, 2610, 3135
MspR9I CCNGG 8 cut(s) 1240, 1322, 1734, 2246, 2544, 2874, 2986, 3229
MunI CAATTG 3 cut(s) 63, 273, 3633
Mva1269I GAATGC 3 cut(s) 1316, 3619, 3854
MvaI CCWGG 8 cut(s) 1240, 1322, 1734, 2246, 2544, 2874, 2986, 3229
MvnI CGCG 1 cut(s) 3500
MwoI GCNNNNNNNGC 6 cut(s) 674, 1322, 1403, 2836, 2990, 3420
NcoI CCATGG 1 cut(s) 3873
NlaIV GGNNCC 9 cut(s) 481, 1146, 1832, 1939, 2250, 3608, 3729, 3818, 3938
NmuCI GTSAC 3 cut(s) 919, 1231, 1297
NsiI ATGCAT 2 cut(s) 1566, 3416
NspI RCATGY 4 cut(s) 712, 1823, 1849, 2467
NspV TTCGAA 1 cut(s) 3081
OliI CACNNNNGTG 1 cut(s) 3287
PaeR7I CTCGAG 2 cut(s) 1986, 4070
PagI TCATGA 1 cut(s) 1470
PciI ACATGT 3 cut(s) 708, 1819, 1845
PcsI WCGNNNNNNNCGW 1 cut(s) 4077
PctI GAATGC 3 cut(s) 1316, 3619, 3854
PdmI GAANNNNTTC 2 cut(s) 2069, 3941
PfeI GAWTC 5 cut(s) 1590, 2114, 2744, 2789, 3786
PflMI CCANNNNNTGG 2 cut(s) 2972, 3415
PfoI TCCNGGA 1 cut(s) 1238
PleI GAGTC 6 cut(s) 926, 2097, 2555, 3141, 3471, 4062
PmaCI CACGTG 1 cut(s) 1151
PmlI CACGTG 1 cut(s) 1151
PpsI GAGTC 6 cut(s) 926, 2097, 2555, 3141, 3471, 4062
Ppu21I YACGTR 2 cut(s) 564, 1151
PpuMI RGGWCCY 1 cut(s) 479
PscI ACATGT 3 cut(s) 708, 1819, 1845
PshBI ATTAAT 1 cut(s) 3186
Psp124BI GAGCTC 1 cut(s) 334
Psp5II RGGWCCY 1 cut(s) 479
Psp6I CCWGG 8 cut(s) 1238, 1320, 1732, 2244, 2542, 2872, 2984, 3227
PspCI CACGTG 1 cut(s) 1151
PspGI CCWGG 8 cut(s) 1238, 1320, 1732, 2244, 2542, 2872, 2984, 3227
PspN4I GGNNCC 9 cut(s) 481, 1146, 1832, 1939, 2250, 3608, 3729, 3818, 3938
PspPI GGNCC 8 cut(s) 121, 479, 944, 1145, 2131, 3137, 3607, 4020
PspPPI RGGWCCY 1 cut(s) 479
PstI CTGCAG 1 cut(s) 1057
PsuI RGATCY 6 cut(s) 538, 545, 1749, 2248, 3367, 3526
PvuII CAGCTG 1 cut(s) 3677
RseI CAYNNNNRTG 6 cut(s) 362, 1781, 2060, 2462, 2594, 3287
SacI GAGCTC 1 cut(s) 334
Sau96I GGNCC 8 cut(s) 121, 479, 944, 1145, 2131, 3137, 3607, 4020
ScaI AGTACT 2 cut(s) 2274, 3173
SchI GAGTC 6 cut(s) 927, 2098, 2556, 3141, 3471, 4062
ScrFI CCNGG 8 cut(s) 1240, 1322, 1734, 2246, 2544, 2874, 2986, 3229
SduI GDGCHC 7 cut(s) 334, 958, 1164, 1267, 1931, 2860, 3578
SexAI ACCWGGT 1 cut(s) 2872
SfaNI GCATC 6 cut(s) 301, 422, 903, 924, 1551, 2971
SfcI CTRYAG 3 cut(s) 1053, 1070, 2109
Sfr274I CTCGAG 2 cut(s) 1986, 4070
SfuI TTCGAA 1 cut(s) 3081
SinI GGWCC 7 cut(s) 479, 944, 1145, 2131, 3137, 3607, 4020
SlaI CTCGAG 2 cut(s) 1986, 4070
SmiMI CAYNNNNRTG 6 cut(s) 362, 1781, 2060, 2462, 2594, 3287
SmlI CTYRAG 7 cut(s) 161, 233, 833, 1745, 1835, 1986, 4070
SmoI CTYRAG 7 cut(s) 161, 233, 833, 1745, 1835, 1986, 4070
SnaBI TACGTA 1 cut(s) 564
SsiI CCGC 5 cut(s) 200, 584, 2294, 3252, 4038
SspI AATATT 1 cut(s) 1545
SspMI CTAG 8 cut(s) 129, 678, 726, 794, 1362, 1455, 2799, 3066
SstI GAGCTC 1 cut(s) 334
StyD4I CCNGG 8 cut(s) 1238, 1320, 1732, 2244, 2542, 2872, 2984, 3227
StyI CCWWGG 4 cut(s) 151, 2074, 2118, 3873
TaiI ACGT 3 cut(s) 566, 1153, 1270
TatI WGTACW 7 cut(s) 1842, 2272, 3171, 3291, 3372, 3684, 3831
TauI GCSGC 2 cut(s) 3254, 4040
TfiI GAWTC 5 cut(s) 1590, 2114, 2744, 2789, 3786
TscAI CASTG 9 cut(s) 657, 669, 1306, 1579, 3030, 3443, 3630, 3660, 4152
TseFI GTSAC 3 cut(s) 919, 1231, 1297
Tsp45I GTSAC 3 cut(s) 919, 1231, 1297
TspRI CASTG 9 cut(s) 657, 669, 1306, 1579, 3030, 3443, 3630, 3660, 4152
Van91I CCANNNNNTGG 2 cut(s) 2972, 3415
VpaK11BI GGWCC 7 cut(s) 479, 944, 1145, 2131, 3137, 3607, 4020
VspI ATTAAT 1 cut(s) 3186
XagI CCTNNNNNAGG 2 cut(s) 1372, 3354
XapI RAATTY 4 cut(s) 587, 1175, 1480, 2069
XbaI TCTAGA 2 cut(s) 725, 1454
XceI RCATGY 4 cut(s) 712, 1823, 1849, 2467
XcmI CCANNNNNNNNNTGG 2 cut(s) 868, 2163
XhoI CTCGAG 2 cut(s) 1986, 4070
XmnI GAANNNNTTC 2 cut(s) 2069, 3941
XspI CTAG 8 cut(s) 129, 678, 726, 794, 1362, 1455, 2799, 3066
ZrmI AGTACT 2 cut(s) 2274, 3173
Zsp2I ATGCAT 2 cut(s) 1566, 3416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.