RLG00000003647
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000493
Physical Location & Seq
Forward (+)
51240579 .. 51249655
9077 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003647

Sequence Viewer

Length: 3957 bp
ATGAACGTCATGATCAGCCAGGAACAAGAAGAAGAGCCAGTCTCCGAGCCTCCTTCCGGTGAAAAGGCCGATAAGGAGTCGAGTCAGACAATGACGACATGGGGTGCCATTGAGAGACTGCCTACGTATTCACGCACAAGGAGAGGCATTCTACTCGGGCAAGGTCAAGAGGAGGACGGTGAAAGCAGAGAGATTGATGTAAAGAAGAATCTTGGACTACCAGAGAGGAAGACTTTGTTGGAGAGGCTAGTGAAGATTGTTGAGCAAGATAATGACAAGTTCTTGCTCAAGATCAAGGACCGCATGAATAGAGTTGGACTTGAATTTCCAACAACTGAAGTGCGGTTCGAGCATTTAAATGTTGAAGCAGAAGCTTATGTAGGAAGCAGGGCATCATCTACAATGTTCAACTTCTCTATCAATATGTTAGAGGGGTTCCTGAATTATTTTCACATTCTTCCAAGTAGAAAGAGACCATTTACAATCCTCCATGATGCAAGTGGGATTATCAAGCCAAGAAGAATGACACTTCTTTTAGGGCCCCCTGGCTCTGGAAAGACGACGTTACTATTGGCCTTGGCTGGAAAACTTGGTAAAGCCAATCTAAAATTTTCGGGGAGAGTTACATACAATGGAATTGAGATAGATCAGTTTGTATCAAAGAGGACATCAGCCTATGTTAGTCAACATGATCTCCACATACCAGAATTGACAGTGAGAGAAACACTGGCTTTTGCAGCTAGATGTCAAGGTGTTGGACCACGTTATGAAATGTTGGTGGAATTACTAAGGAGAGAAAAGGCTGCAAATATTATGCCAGATCTTGATCTTGATCTCTACGTGAAGGCGGCAACACTAGAAGGACAGGAAACTAATATTGTTACAGACTTTATTCTCAAGGTTTTGGGACTCGAAGATTGTGCTGAAATTATGGTAGGAGATGAAATGACCAGAGGTATCTCTGGTGGACAAAAAAAGCGACTCACCACAGGGGAGATGCTGGTTGGACCAGAAAGAGTACTTCTCATGGATGAGATATCAAACGGGCTGGACAGTTCAACAACATTGCAAATAGTGAAATCGCTACAACAATACGTCCACATCCTCAATGGAACTGCACTAATCGCTCTACTGCAGCCAGCACCAGAGACTTATGATCTCTTCGATGATATAATTCTCCTTTCGGATGGCTACATTGTGTATCAAGGTCCCCGTGAGAATGTTCTTGAGTTCTTTGAGTACATGGGCTTCAAATGTCCAGAGCGGAAAGGAGTTGCTGATTTCCTACAAGAGGTGACATCAAGAAAAGATCAAGAGCAATACTGGGCTCATATGGATAGGCCATATAGCTTTGTAACAACCAAAGAATTTTCTGAAGCAATGCAGTTATTTCACATTGGTAGAAAACTTGGTGACGAACTTTCTATTCCATTTAACAAGTCAGAAGGCTGCCCTGGTGCTCTGTCAACTAAAAAATATGGTGTTAGTAAGAAGGAATTGTTCAAAGCTTGTATGGCTAGACAAATTTTGCTTACAAAGAGGAATTCATTTGTCTACATTTTCAAATTGGTGCAGCTTATTCTAGTTGCTGTTACAACAATGACACTATTTTTTCATACTGAGATGGAGCGAAACACAGTTGCAGATGGTAGGATTTATATGGGAGCTTTATTCTATACGCTACTCGCAATTATGCTTAATGGGTTTCCAGAGCTTCATATGACAGTCACTAGACTGCCTGTGTTTTTCAAGCAAAGGGACCATCTGTTCTATCCTGCTTGGGCATACTCCTTACCCCCATGGCTGATAAGGATCCCTATAACTTTTGTGGACGTTTTCATCTGGGTGATCATTACTTACTACAGTGTAGGTTATGATCCGAGCGTTCCTGCCAACTCAACAGAGGCATTAGGAGTTATGGTCTTGAAGTATCAAGGCATATTCCCAGAGGCACGCTTCTACTGGATTGGATTAGTAGCTTTGATTGGATTTATTCTTCTGTTGAATTTCCTTTTCACCTTGGCACTTCAGTATCTTGATCCACTTGAGATGCCCCGAGCAGTACTATCCAATGACGCATCCTCTACAACAGAGGATAGCAGAGTTGCAAGCAATCCTAGTATGCAGCATGGCATGGTTCTTCCATTTGAACCCCTTTCAATTACTTTCGAGGATATCAGATATGCTGTTGATATGCCTGAGGAAATGAAAGATCAAAGCGAAACTCGAGATCGGCTAGAACTTCTGAAGGGTGTGAGTGGTACTTTTAGGCCTGGAGTTCTAACAGCTTTGATGGGTGTTAGTGGTGCTGGGAAAACCACTCTCATGGATGTCTTGGCAGGAAGGAAGACAAGTGGATATATTGAGGGGAGGATCACCTTATCTGGCTATGCAAAAAGGCAAGAAACATTTGCTCGCATATCAGGATACTGTGAGCAAACAGATATACACTCCCCTCATGTCACAGTTTACGAGTCTTTGGTTTACTCTGCATGGCTCCGGTTGCCCCAGGAGGTTGATTCCCCAACCAGAAAGATGTTTATTGAGGAAGTCATGGAGCTTGTAGAAATGACCTCAATAAGTGACAGACTTGTTGGATTGCCAGGAGTGAATGGTCTCTCAACTGAACAACGCAAAAGGATAACAATTGCAGTAGAGCTTGTTGCCAACCCCTCGATAATATTTATGGATGAGCCGACTTCTGGCCTCGATGCTAGGGCAGCAGCAATTGTAATGAGAACGGTGAGGAATACAGTGGACACTGGGAGAACTGTAGTCTGTACCATCCACCAACCAAGCATTGATATATTTGATGCTTTTGATGAGATGTTGCTTCTGAAACAGGGAGGCGAAGAAATATATGTTGGCCCTTTAGGCCACCATTCTTCCCAGATGATTGAATACTTTGAGGAGATTAGTGGAGTTTCTAAAATCAAGGATGGTTACAATCCAGCAACCTGGATGTTGGAGGTTACTTCTCCAGCACAAGAAGCATCTCTTGGGGTTGATTTTGCCAACATATACAAGAATTCAGAAATATATAGGACAAATAAGGCATTGATAAAGGAACTAAGTACTCCTGCACCAAATTCAAAGGACTTGTACTTCCCAACACAATATTCGCAGTCCTTCTTTACCCAGTGTCTAGCTTGCCTATGGAAACAGAGTGTATCATATTGGCGAAACTCTCAATACACTGCAGTGAGACTTCTTTTCACAGCTATGATGGCCGGACTACTTGGGTCATTTTTTTGGGATCTTGGCTCCAAAAGACAAAAGCAAAGAGATCTCTTTTGTGCGATGGGTTCTATGTATGCCGCTGTGCTCTTTATTGGTATACAAAATTCCTTGTCAGTGCAGCCAATCGTAGGTGTTGAGAGGTTGGTCTCTTACAGAGAAACGGCTGCTGGAATGTACTCAGCTTTTCCATTTGCCTTTGGACAGGCTATCATTGAGATCCCATACACTTTGATCCAGACTATTATATTTGGGGTTATAGTATACTCCATGGTCGGATTTGATTGGACAGTCAGCAAGTTCTTTTGTTATCTCTTGTTCATGTACCTCACCTTCTTATACTTCACCTTCCATGGCATGATGATTGTGGCCATTACTCCCAACAACACCATCTCTGCTGTAGTTTCGTCTGCCTTCTATCCATTATGGAATGTTATTTCAGGATATCTCATTCCCAAAACAAGAATTCCAATATGGTGGAGATGGTTCTATTGGGTCAGCCCGACCTCTTGGAGCTTGTATGGATTGTTTTCTTCACAGTTTGGAGGTCTCAAGGACACACTTGATTCTGGTGAAACTGTAGATCATTTTATGAGGACATATTTTGGATATAGGAAAGACTTTCTAGGTGTTGTTGTAGCTGTGCTTGTTGGGTTTTCAGCGTTCTTTGTTTTCGTCTTTGCTTTTGCAATCAAGAAACTAAACTTCCAAAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1319

Amino Acids

149.13

Weight (kDa)

6.01

Isoelectric Point (pI)

40.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 88 - 138 7.9e-06 ABC-transporter N-terminal
ABC_tran PF00005 163 - 346 2.5e-17 ABC transporter
ABC2_membrane PF01061 500 - 644 8.3e-21 ABC-2 type transporter
PDR_assoc PF08370 630 - 688 9.8e-22 Plant PDR ABC transporter associated
ABC_tran PF00005 749 - 901 1.6e-21 ABC transporter
ABC2_membrane PF01061 1046 - 1260 7.2e-53 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000493)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29820 FvH4_2g29820 FvH4_2g29822 FvH4_2g29824 FvH4_2g29824 FvH4_2g29824 FvH4_2g29840 FvH4_2g29840 FvH4_2g29840 FvH4_5g00550 FvH4_5g00550
malus_domestica MD02G1032700.v1.1 MD08G1140900.v1.1 MD08G1141400.v1.1 MD15G1117800.v1.1 MD15G1117900.v1.1
prunus_persica Prupe.1G471200_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471400_v2.0.a1 Prupe.7G242900_v2.0.a1
pyrus_communis pycom02g02690 pycom08g11890
rosa_chinensis RchiOBHm_Chr2g0111741 RchiOBHm_Chr2g0145991 RchiOBHm_Chr6g0298851 RchiOBHm_Chr6g0298871 RchiOBHm_Chr6g0298891 RchiOBHm_Chr6g0298901 RchiOBHm_Chr6g0298911 RchiOBHm_Chr7g0201801 RchiOBHm_Chr7g0211531 RchiOBHm_Chr7g0214601
rosa_laevigata RLG00000002931 RLG00000003000 RLG00000003647 RLG00000011476 RLG00000011479
rosa_multiflora Rmu_sc0001030.1_g000012 Rmu_sc0001548.1_g000041 Rmu_ssc0000397.1_g000033 Rmu_ssc0000397.1_g000036 Rmu_ssc0000397.1_g000037 Rmu_ssc0000397.1_g000039 Rmu_ssc0000397.1_g000048
rosa_roxburghii Rroxscaffold_1G00003830 Rroxscaffold_3G00247810 Rroxscaffold_3G00255060 Rroxscaffold_7G00169220 Rroxscaffold_7G00169230 Rroxscaffold_7G00169280
rosa_rugosa Rorug02G0223600 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283200 Rorug06G0283300 Rorug06G0283400 Rorug06G0283500 Rorug07G0066000
rosa_samantha Rh2AG511300 Rh2CG235100 Rh2CG496400 Rh2DG330900 Rh6BG403100 Rh6BG403300 Rh6BG403400 Rh6BG403500 Rh6CG408700 Rh6CG408900 Rh6CG409000 Rh6CG409200 Rh6DG395800 Rh6DG395900 Rh6DG396000 Rh6DG396200 Rh7AG193400 Rh7BG195100 Rh7BG436600 Rh7DG199300 Rh7DG265500
rosa_wichuraiana Rw2G016900 Rw6G034570 Rw6G034580 Rw6G034590 Rw6G034600 Rw7G016900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 104
AccBSI CCGCTC 1 cut(s) 1264
AccI GTMKAC 3 cut(s) 1554, 3337, 3501
AciI CCGC 5 cut(s) 301, 343, 848, 1264, 3318
AclWI GGATC 8 cut(s) 1807, 1820, 1871, 2033, 2381, 3264, 3451, 3466
AcoI YGGCCR 2 cut(s) 3228, 3606
AcuI CTGAAG 4 cut(s) 357, 1395, 2012, 2267
AfaI GTAC 9 cut(s) 1020, 1241, 2064, 2263, 2782, 3076, 3104, 3416, 3563
AfiI CCNNNNNNNGG 5 cut(s) 56, 551, 1291, 2702, 3368
AjnI CCWGG 7 cut(s) 18, 544, 1453, 2272, 2508, 2602, 2957
AjuI GAANNNNNNNTTGG 8 cut(s) 221, 253, 2847, 2879, 2982, 3014, 3103, 3135
AleI CACNNNNGTG 1 cut(s) 3200
AloI GAACNNNNNNTCC 2 cut(s) 1751, 1783
Alw21I GWGCWC 2 cut(s) 1462, 3327
Alw26I GTCTC 8 cut(s) 46, 109, 466, 1142, 2621, 3199, 3391, 3791
AlwI GGATC 8 cut(s) 1807, 1820, 1871, 2033, 2381, 3264, 3451, 3466
Ama87I CYCGRG 3 cut(s) 155, 2055, 2226
ApaI GGGCCC 1 cut(s) 543
AspS9I GGNCC 8 cut(s) 298, 539, 540, 758, 1007, 1208, 1759, 2867
AvaI CYCGRG 3 cut(s) 155, 2055, 2226
AvaII GGWCC 5 cut(s) 298, 758, 1007, 1208, 1759
AxyI CCTNAGG 1 cut(s) 2199
BaeGI GKGCMC 1 cut(s) 543
BaeI ACNNNNGTAYC 2 cut(s) 2015, 2048
BalI TGGCCA 1 cut(s) 3608
BamHI GGATCC 1 cut(s) 1812
BanI GGYRCC 1 cut(s) 104
BanII GRGCYC 2 cut(s) 543, 1330
BarI GAAGNNNNNNTAC 4 cut(s) 1232, 1264, 3554, 3586
BbsI GAAGAC 2 cut(s) 236, 2354
Bbv12I GWGCWC 2 cut(s) 1462, 3327
BceAI ACGGC 1 cut(s) 3417
BcgI CGANNNNNNTGC 4 cut(s) 136, 170, 902, 936
BciT130I CCWGG 7 cut(s) 20, 546, 1455, 2274, 2510, 2604, 2959
BciVI GTATCC 1 cut(s) 2420
BclI TGATCA 2 cut(s) 12, 1848
BcoDI GTCTC 8 cut(s) 46, 109, 466, 1142, 2621, 3199, 3391, 3791
BfmI CTRYAG 6 cut(s) 1133, 1861, 2772, 3198, 3636, 3816
BfuI GTATCC 1 cut(s) 2420
BglI GCCNNNNNGGC 1 cut(s) 2874
BglII AGATCT 2 cut(s) 820, 3286
BmcAI AGTACT 3 cut(s) 1020, 2064, 3076
Bme1390I CCNGG 7 cut(s) 20, 546, 1455, 2274, 2510, 2604, 2959
Bme18I GGWCC 5 cut(s) 298, 758, 1007, 1208, 1759
BmeT110I CYCGRG 3 cut(s) 155, 2055, 2226
BmgT120I GGNCC 8 cut(s) 298, 539, 540, 758, 1007, 1208, 1759, 2867
BmiI GGNNCC 9 cut(s) 106, 437, 541, 542, 1210, 1760, 1814, 2498, 3265
BmrFI CCNGG 7 cut(s) 20, 546, 1455, 2274, 2510, 2604, 2959
BmrI ACTGGG 3 cut(s) 1333, 2772, 3133
BmsI GCATC 8 cut(s) 401, 484, 987, 2040, 2087, 2701, 2803, 3002
BmuI ACTGGG 3 cut(s) 1333, 2772, 3133
BpiI GAAGAC 2 cut(s) 236, 2354
BplI GAGNNNNNCTC 4 cut(s) 26, 58, 1008, 1040
BpmI CTGGAG 2 cut(s) 2295, 2964
BpuEI CTTGAG 5 cut(s) 272, 881, 1247, 2066, 3773
BsaAI YACGTR 2 cut(s) 126, 841
BsaBI GATNNNNATC 3 cut(s) 825, 831, 1811
BsaI GGTCTC 4 cut(s) 466, 2621, 3391, 3791
BsaJI CCNNGG 8 cut(s) 544, 576, 1453, 1799, 2019, 2508, 3507, 3589
BsaWI WCCGGW 2 cut(s) 56, 2499
BsaXI ACNNNNNCTCC 4 cut(s) 678, 708, 1905, 1935
Bsc4I CCNNNNNNNGG 5 cut(s) 56, 551, 1291, 2702, 3368
Bse1I ACTGG 6 cut(s) 38, 732, 1328, 1967, 2767, 3139
Bse21I CCTNAGG 1 cut(s) 2199
Bse3DI GCAATG 2 cut(s) 1064, 1386
Bse8I GATNNNNATC 3 cut(s) 825, 831, 1811
BseBI CCWGG 7 cut(s) 20, 546, 1455, 2274, 2510, 2604, 2959
BseDI CCNNGG 8 cut(s) 544, 576, 1453, 1799, 2019, 2508, 3507, 3589
BseGI GGATG 9 cut(s) 1036, 1101, 1192, 2078, 2335, 2695, 2784, 2944, 2967
BseJI GATNNNNATC 3 cut(s) 825, 831, 1811
BseLI CCNNNNNNNGG 5 cut(s) 56, 551, 1291, 2702, 3368
BseMI GCAATG 2 cut(s) 1064, 1386
BseMII CTCAG 3 cut(s) 1611, 2190, 3432
BseNI ACTGG 6 cut(s) 38, 732, 1328, 1967, 2767, 3139
BseRI GAGGAG 2 cut(s) 185, 2924
BseSI GKGCMC 1 cut(s) 543
BseYI CCCAGC 1 cut(s) 2309
BsgI GTGCAG 4 cut(s) 1101, 1592, 3066, 3377
BshNI GGYRCC 1 cut(s) 104
BsiHKAI GWGCWC 2 cut(s) 1462, 3327
BsiHKCI CYCGRG 3 cut(s) 155, 2055, 2226
BsiSI CCGG 3 cut(s) 57, 2500, 3231
BslFI GGGAC 3 cut(s) 921, 1194, 1772
BslI CCNNNNNNNGG 5 cut(s) 56, 551, 1291, 2702, 3368
BsmAI GTCTC 8 cut(s) 46, 109, 466, 1142, 2621, 3199, 3391, 3791
BsmFI GGGAC 3 cut(s) 921, 1194, 1772
BsmI GAATGC 1 cut(s) 147
Bso31I GGTCTC 4 cut(s) 466, 2621, 3391, 3791
BsoBI CYCGRG 3 cut(s) 155, 2055, 2226
Bsp120I GGGCCC 1 cut(s) 539
Bsp1286I GDGCHC 4 cut(s) 543, 1330, 1462, 3327
Bsp19I CCATGG 3 cut(s) 1799, 3507, 3589
BspACI CCGC 5 cut(s) 301, 343, 848, 1264, 3318
BspCNI CTCAG 3 cut(s) 1612, 2191, 3431
BspHI TCATGA 1 cut(s) 9
BspLI GGNNCC 9 cut(s) 106, 437, 541, 542, 1210, 1760, 1814, 2498, 3265
BspMAI CTGCAG 2 cut(s) 1137, 3202
BspPI GGATC 8 cut(s) 1807, 1820, 1871, 2033, 2381, 3264, 3451, 3466
BspQI GCTCTTC 1 cut(s) 27
BspT107I GGYRCC 1 cut(s) 104
BspTNI GGTCTC 4 cut(s) 466, 2621, 3391, 3791
BsrBI CCGCTC 1 cut(s) 1264
BsrDI GCAATG 2 cut(s) 1064, 1386
BsrI ACTGG 6 cut(s) 38, 732, 1328, 1967, 2767, 3139
BssECI CCNNGG 8 cut(s) 544, 576, 1453, 1799, 2019, 2508, 3507, 3589
BssNAI GTATAC 2 cut(s) 3338, 3502
BssT1I CCWWGG 5 cut(s) 576, 1799, 2019, 3507, 3589
Bst1107I GTATAC 2 cut(s) 3338, 3502
Bst2UI CCWGG 7 cut(s) 20, 546, 1455, 2274, 2510, 2604, 2959
Bst6I CTCTTC 2 cut(s) 27, 1166
BstBAI YACGTR 2 cut(s) 126, 841
BstC8I GCNNGC 5 cut(s) 1140, 1954, 2110, 2416, 3151
BstDEI CTNAG 5 cut(s) 788, 1620, 2199, 3071, 3418
BstDSI CCRYGG 3 cut(s) 1799, 3507, 3589
BstENI CCTNNNNNAGG 1 cut(s) 1289
BstF5I GGATG 9 cut(s) 1036, 1101, 1192, 2078, 2335, 2695, 2784, 2944, 2967
BstMAI GTCTC 8 cut(s) 46, 109, 466, 1142, 2621, 3199, 3391, 3791
BstMWI GCNNNNNNNGC 9 cut(s) 349, 737, 1124, 1514, 2503, 2720, 2874, 2990, 3227
BstNI CCWGG 7 cut(s) 20, 546, 1455, 2274, 2510, 2604, 2959
BstSCI CCNGG 7 cut(s) 18, 544, 1453, 2272, 2508, 2602, 2957
BstSFI CTRYAG 6 cut(s) 1133, 1861, 2772, 3198, 3636, 3816
BstSLI GKGCMC 1 cut(s) 543
BstSNI TACGTA 1 cut(s) 126
BstV2I GAAGAC 2 cut(s) 236, 2354
BstX2I RGATCY 5 cut(s) 820, 1812, 3256, 3286, 3456
BstXI CCANNNNNNTGG 3 cut(s) 2326, 2958, 3714
BstYI RGATCY 5 cut(s) 820, 1812, 3256, 3286, 3456
BstZ17I GTATAC 2 cut(s) 3338, 3502
Bsu36I CCTNAGG 1 cut(s) 2199
BsuI GTATCC 1 cut(s) 2420
BtgI CCRYGG 3 cut(s) 1799, 3507, 3589
BtgZI GCGATG 1 cut(s) 3314
BtsCI GGATG 9 cut(s) 1036, 1101, 1192, 2078, 2335, 2695, 2784, 2944, 2967
BtsI GCAGTG 2 cut(s) 3195, 3207
BtsIMutI CAGTG 9 cut(s) 720, 725, 1870, 2760, 2760, 3146, 3195, 3207, 3360
Cac8I GCNNGC 5 cut(s) 1140, 1954, 2110, 2416, 3151
CciI TCATGA 1 cut(s) 9
Cfr13I GGNCC 8 cut(s) 298, 539, 540, 758, 1007, 1208, 1759, 2867
CseI GACGC 1 cut(s) 2084
Csp6I GTAC 9 cut(s) 1019, 1240, 2063, 2262, 2781, 3075, 3103, 3415, 3562
CviQI GTAC 9 cut(s) 1019, 1240, 2063, 2262, 2781, 3075, 3103, 3415, 3562
DdeI CTNAG 5 cut(s) 788, 1620, 2199, 3071, 3418
DraI TTTAAA 1 cut(s) 357
EaeI YGGCCR 2 cut(s) 3228, 3606
Eam1104I CTCTTC 2 cut(s) 27, 1166
EarI CTCTTC 2 cut(s) 27, 1166
Eco105I TACGTA 1 cut(s) 126
Eco130I CCWWGG 5 cut(s) 576, 1799, 2019, 3507, 3589
Eco147I AGGCCT 1 cut(s) 2272
Eco24I GRGCYC 2 cut(s) 543, 1330
Eco31I GGTCTC 4 cut(s) 466, 2621, 3391, 3791
Eco32I GATATC 3 cut(s) 1038, 2176, 3683
Eco47I GGWCC 5 cut(s) 298, 758, 1007, 1208, 1759
Eco57I CTGAAG 4 cut(s) 357, 1395, 2012, 2267
Eco81I CCTNAGG 1 cut(s) 2199
Eco88I CYCGRG 3 cut(s) 155, 2055, 2226
EcoNI CCTNNNNNAGG 1 cut(s) 1289
EcoO109I RGGNCCY 3 cut(s) 539, 540, 1208
EcoRI GAATTC 3 cut(s) 1543, 3028, 3702
EcoRII CCWGG 7 cut(s) 18, 544, 1453, 2272, 2508, 2602, 2957
EcoRV GATATC 3 cut(s) 1038, 2176, 3683
EcoT14I CCWWGG 5 cut(s) 576, 1799, 2019, 3507, 3589
EcoT38I GRGCYC 2 cut(s) 543, 1330
ErhI CCWWGG 5 cut(s) 576, 1799, 2019, 3507, 3589
FalI AAGNNNNNCTT 2 cut(s) 2982, 3014
FaqI GGGAC 3 cut(s) 921, 1194, 1772
FauNDI CATATG 2 cut(s) 1332, 1719
FbaI TGATCA 2 cut(s) 12, 1848
FblI GTMKAC 3 cut(s) 1554, 3337, 3501
FokI GGATG 9 cut(s) 1043, 1088, 1199, 2065, 2342, 2702, 2771, 2951, 2974
FriOI GRGCYC 2 cut(s) 543, 1330
GsaI CCCAGC 1 cut(s) 2313
GsuI CTGGAG 2 cut(s) 2295, 2964
HapII CCGG 3 cut(s) 57, 2500, 3231
HgaI GACGC 1 cut(s) 2084
HincII GTYRAC 2 cut(s) 686, 1467
HindII GTYRAC 2 cut(s) 686, 1467
HindIII AAGCTT 2 cut(s) 372, 1506
HinfI GANTC 8 cut(s) 77, 82, 208, 909, 981, 2474, 2519, 3803
HpaII CCGG 3 cut(s) 57, 2500, 3231
Hpy99I CGWCG 1 cut(s) 565
HpyCH4IV ACGT 7 cut(s) 6, 125, 563, 763, 840, 1095, 1833
HpyF10VI GCNNNNNNNGC 9 cut(s) 349, 737, 1124, 1514, 2503, 2720, 2874, 2990, 3227
HpyF3I CTNAG 5 cut(s) 788, 1620, 2199, 3071, 3418
HpySE526I ACGT 7 cut(s) 6, 125, 563, 763, 840, 1095, 1833
Ksp22I TGATCA 2 cut(s) 12, 1848
LguI GCTCTTC 1 cut(s) 27
LmnI GCTCC 6 cut(s) 1627, 1664, 2502, 2557, 3269, 3750
LweI GCATC 8 cut(s) 401, 484, 987, 2040, 2087, 2701, 2803, 3002
MaeII ACGT 7 cut(s) 6, 125, 563, 763, 840, 1095, 1833
MbiI CCGCTC 1 cut(s) 1264
MfeI CAATTG 2 cut(s) 2646, 2727
MflI RGATCY 5 cut(s) 820, 1812, 3256, 3286, 3456
MhlI GDGCHC 4 cut(s) 543, 1330, 1462, 3327
MlsI TGGCCA 1 cut(s) 3608
MluNI TGGCCA 1 cut(s) 3608
MlyI GAGTC 5 cut(s) 86, 91, 903, 975, 2483
MmeI TCCRAC 8 cut(s) 219, 295, 353, 736, 985, 2575, 2946, 3493
Mox20I TGGCCA 1 cut(s) 3608
MscI TGGCCA 1 cut(s) 3608
MseI TTAA 3 cut(s) 356, 1434, 1698
MslI CAYNNNNRTG 5 cut(s) 357, 1844, 2324, 3200, 3221
Msp20I TGGCCA 1 cut(s) 3608
MspA1I CMGCKG 1 cut(s) 3320
MspI CCGG 3 cut(s) 57, 2500, 3231
MspR9I CCNGG 7 cut(s) 20, 546, 1455, 2274, 2510, 2604, 2959
MunI CAATTG 2 cut(s) 2646, 2727
Mva1269I GAATGC 1 cut(s) 147
MvaI CCWGG 7 cut(s) 20, 546, 1455, 2274, 2510, 2604, 2959
MwoI GCNNNNNNNGC 9 cut(s) 349, 737, 1124, 1514, 2503, 2720, 2874, 2990, 3227
NcoI CCATGG 3 cut(s) 1799, 3507, 3589
NdeI CATATG 2 cut(s) 1332, 1719
NlaIV GGNNCC 9 cut(s) 106, 437, 541, 542, 1210, 1760, 1814, 2498, 3265
NmuCI GTSAC 5 cut(s) 1294, 1412, 1726, 2461, 2582
OliI CACNNNNGTG 1 cut(s) 3200
PaeR7I CTCGAG 1 cut(s) 2226
PagI TCATGA 1 cut(s) 9
PceI AGGCCT 1 cut(s) 2272
PciSI GCTCTTC 1 cut(s) 27
PctI GAATGC 1 cut(s) 147
PfeI GAWTC 3 cut(s) 208, 2519, 3803
PleI GAGTC 5 cut(s) 85, 90, 903, 975, 2482
PpsI GAGTC 5 cut(s) 85, 90, 903, 975, 2482
Ppu21I YACGTR 2 cut(s) 126, 841
PpuMI RGGWCCY 1 cut(s) 1208
Psp5II RGGWCCY 1 cut(s) 1208
Psp6I CCWGG 7 cut(s) 18, 544, 1453, 2272, 2508, 2602, 2957
PspFI CCCAGC 1 cut(s) 2309
PspGI CCWGG 7 cut(s) 18, 544, 1453, 2272, 2508, 2602, 2957
PspN4I GGNNCC 9 cut(s) 106, 437, 541, 542, 1210, 1760, 1814, 2498, 3265
PspOMI GGGCCC 1 cut(s) 539
PspPI GGNCC 8 cut(s) 298, 539, 540, 758, 1007, 1208, 1759, 2867
PspPPI RGGWCCY 1 cut(s) 1208
PstI CTGCAG 2 cut(s) 1137, 3202
PsuI RGATCY 5 cut(s) 820, 1812, 3256, 3286, 3456
RsaI GTAC 9 cut(s) 1020, 1241, 2064, 2263, 2782, 3076, 3104, 3416, 3563
RsaNI GTAC 9 cut(s) 1019, 1240, 2063, 2262, 2781, 3075, 3103, 3415, 3562
RseI CAYNNNNRTG 5 cut(s) 357, 1844, 2324, 3200, 3221
SapI GCTCTTC 1 cut(s) 27
SaqAI TTAA 3 cut(s) 356, 1434, 1698
Sau96I GGNCC 8 cut(s) 298, 539, 540, 758, 1007, 1208, 1759, 2867
ScaI AGTACT 3 cut(s) 1020, 2064, 3076
SchI GAGTC 5 cut(s) 86, 91, 903, 975, 2483
ScrFI CCNGG 7 cut(s) 20, 546, 1455, 2274, 2510, 2604, 2959
SduI GDGCHC 4 cut(s) 543, 1330, 1462, 3327
SfaNI GCATC 8 cut(s) 401, 484, 987, 2040, 2087, 2701, 2803, 3002
SfcI CTRYAG 6 cut(s) 1133, 1861, 2772, 3198, 3636, 3816
SfiI GGCCNNNNNGGCC 1 cut(s) 2874
Sfr274I CTCGAG 1 cut(s) 2226
SinI GGWCC 5 cut(s) 298, 758, 1007, 1208, 1759
SlaI CTCGAG 1 cut(s) 2226
SmiI ATTTAAAT 1 cut(s) 357
SmiMI CAYNNNNRTG 5 cut(s) 357, 1844, 2324, 3200, 3221
SmlI CTYRAG 6 cut(s) 287, 896, 1226, 2045, 2226, 3788
SmoI CTYRAG 6 cut(s) 287, 896, 1226, 2045, 2226, 3788
SnaBI TACGTA 1 cut(s) 126
SseBI AGGCCT 1 cut(s) 2272
SsiI CCGC 5 cut(s) 301, 343, 848, 1264, 3318
SspI AATATT 4 cut(s) 811, 877, 2682, 3119
StuI AGGCCT 1 cut(s) 2272
StyD4I CCNGG 7 cut(s) 18, 544, 1453, 2272, 2508, 2602, 2957
StyI CCWWGG 5 cut(s) 576, 1799, 2019, 3507, 3589
SwaI ATTTAAAT 1 cut(s) 357
TaiI ACGT 7 cut(s) 9, 128, 566, 766, 843, 1098, 1836
TaqI TCGA 8 cut(s) 80, 348, 912, 1164, 2169, 2227, 2675, 2709
TatI WGTACW 6 cut(s) 1018, 1239, 2062, 3074, 3102, 3414
TauI GCSGC 2 cut(s) 851, 3320
TfiI GAWTC 3 cut(s) 208, 2519, 3803
Tru1I TTAA 3 cut(s) 356, 1434, 1698
Tru9I TTAA 3 cut(s) 356, 1434, 1698
TscAI CASTG 9 cut(s) 720, 732, 1870, 2760, 2767, 3146, 3202, 3207, 3360
TseFI GTSAC 5 cut(s) 1294, 1412, 1726, 2461, 2582
Tsp45I GTSAC 5 cut(s) 1294, 1412, 1726, 2461, 2582
TspRI CASTG 9 cut(s) 720, 732, 1870, 2760, 2767, 3146, 3202, 3207, 3360
VpaK11BI GGWCC 5 cut(s) 298, 758, 1007, 1208, 1759
XagI CCTNNNNNAGG 1 cut(s) 1289
XcmI CCANNNNNNNNNTGG 2 cut(s) 497, 1106
XhoI CTCGAG 1 cut(s) 2226
XmiI GTMKAC 3 cut(s) 1554, 3337, 3501
ZrmI AGTACT 3 cut(s) 1020, 2064, 3076
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.