MD08G1141400.v1.1
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000493
Physical Location & Seq
Reverse (-)
13916513 .. 13924043
7531 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1141400.v1.1.491

Sequence Viewer

Length: 4356 bp
ATGGATAACGGTAGTGGTGATATAATTAGAGTGAGTAGTGCTCGTTTGAGCAGTTCTAATATTTGGAGGAACAGCACCATGGATGTTTTCTCCAAGTCTTCACACGATGAAGATGATGAGGAAGCTCTGAAATGGGCAGCCATTGAGAAGCTGCCTACTTATTTGCGCATTCGGCGCGGAATTCTCACCGAGGAAGAAGGCAAAGGAAGAGAGATTGACGTCAAGAATCTTGGGTTGCTTGAAAGAAAGAATGTGCTGGAGAGGCTGGTGAAAACTGCAGAAGAAGACAATGAGAAGTTCTTGTTGAAGCTCAAGGACCGGATTAATCGGGTTGGACTTGATATGCCAACAATTGAAGTGCGTTATGAGCATTTAAATGTTGAAGCAGAAGCTTATGTGGGAGGCAGGGCTTTACCTACAATATTCAATTTTGTTGCGAATATTTTGGAGGGGTGCCTGAATTTTGTTCACATTCTTCCAAGTAGAAAGCATCCGCTACCAATGCTTACTGATGTTAGTGGAATTATCAAGCCTAGAAGAATGACGCTGCTTTTAGGACCCCCAGGCTCTGGGAAAACCACATTACTATTGGCTTTGGCTGGAAAACTTGCTAAAGATCTAAAATTTTCTGGGAGAGTTGCATATAACGGACATGGGATGGAAGAGTTTGTGCCAGAGAGGACCTCGGCTTATATTAGTCAGCATGATCTCCACATAGGGGAAATGACAGTGAGAGAAACACTGGCTTTTTCCGCAAGATGTCAAGGGGTCGGACCACGTTATGAAATGTTGGCAGAATTATCAAGGAGAGAAAAGGAAGCAAATATCATGCCAGATCCTGATGTAGATATCTACATGAAGGCAGCATCGCTGGAAGGACAGGAGACCAGTGTAGTTACAGATTATATACTCAAGGTTTTGGGACTTGAGGTTTGCGCAGACATCATGGTGGGGGACGAAATGGTACGAGGTATTTCTGGCGGACAAAAGAAGCGAGTCACAACAGGGGAGATGCTGGTTGGACCGGCAAGAGCACTTTTTATGGATGAGATATCAACTGGTTTAGACAGTTCAACAACATTTCAGATAGTGAAATCACTCAGAGAATCCATCCACATCCTCAGTGGAACTGCTGTTATCTCTCTTCTCCAACCGGCACCAGAAACCTACGACCTATTTGATGACATAATTGTGCTGTCTGATGGGCAAATCGTGTACCAAGGTCCCCGCGAAAATGTTCTCGAGTTCTTTGAGCACATGGGATTCAAATGTCCAGAGAGGAAAGGAGTAGCTGACTTCTTACAAGAAGTAACATCAAAGAAAGATCAGGAGCAGTACTGGGCGGATAAAGAAGAGCCATATAATTTTATATCTTCCAAGGAATTTGCTGAAGCATTTCAGTCATTTCATATTGGTCGAAAACTTGGTGATGAGCTTGCTACTCCATTTGACAAGTCCAAGGGTCACCCTGCAGCCTTAACAACTCAGAAGTATGGTGTTAGCAAGAAGGAATTGCTTAAAGCTTGCATTGATAGAGAATTTTTGCTTATGAAGAGGAATTCGTTCGTCTACATTTTCAAGATGACTCAGCTCACTCTATTGGCTTTTATATCAATGACACTATTTCTCCGGACTGAGATGCATAAGAATACAGTTGACGATGGGGGTATTTTCATGGGTGCTATGTTCTTTACAGTGATCATAGTTATGTTTAATGGGTTCTCAGAGCTTGCCATGACTATCATGAAACTTCCTGTATTTTTCAAGCAAAGGGACCTTCTCTTCTATCCTGCTTGGGCATACTCTTTACCGACATGGATCCTTAAGATCCCTATCAGTTTCGTAGAAGTTGCCGTTTGGGTGGTCATGACGTACTATGTAATTGGTTTTGATCCAAACATCGAAAGGTTTTTTAGACAATATCTTTTGCTCCTATTGCTTAACCAGATGGCATCAGGACTATTCCGATTTATGGGGGCATTAGGGAGGAATATAATTGTTGCAAACACATTCGGGTCTTTCGCGTTGCTTGCAGTACTAGTTTTGGGTGGCTTTGTCTTGTCTCGAGACGATGTGAAGAAGTGGTGGGTATGGGGTTATTGGGCCTCACCGATGATGTATGGCCAAAACGCCATAGCTGTCAATGAATTTCTCGGAGACAGTTGGAGCCATGTTCCTCCTAATTCAACAGAATCGTTAGGAATTATGGTCTTGAAGTCTCGCGGGGTTTTCATAGAACCATATTGGTACTGGATTGGAGTGGGAGCTACAGTCGGATATATTTTTCTATTCAATTTCTTCTATACTTTGGCCTTGAAGTTTCTCAATCCATTTGAGAAGCCTCAAGCAGTACTATCCAAAGAGGCCTTGGCTGAGAAACTTACTGATCAAACTGGGGATTCTGTTGAGCTATCATCAAGAGGGAAGAACTCTTCTGACCCACGGAATGAAAGTCGAAGAAGTGTCTCATTGAGAACACTGTCTGCAAGAGTGGGTAGCATTACTGAAGCCAATCAAAACAAGAAGCGAGGAATGGTTCTTCCTTTCGAACCTCTTTGGATTACTTTTGATGAAATCAAATATGCTGTTGACATGCCCCAGGAAATGAAAACTCAAGGGGTAACTGAGGATCGGCTACCACTTCTGAAGGGTGTGACCGGTGCTTTTAGGCCGGGAGTCTTAACAGCTCTGATGGGTATTAGTGGCGCTGGAAAGACTACTCTAATGGATGTGTTGGCAGGGAGGAAAACGGGTGGATTTATTGAAGGAAACATCACAATATCTGGGTATCCTAAAAAGCAAGAAACATTTGCTCGCATATCAGGATATTGTGAGCAAACCGATATACACTCTCCTCATGTAACAGTTTATGAGTCTTTGGTTTACTCTGCATCCCTCCGGTTGCCTCCTGAGGTTGATTCCGGTACCAGAAAGATGTTTATTGAGGAAGTCATGGAGCTTGTGGAACTGACCTCGATAAGAGAAGCGCTTGTTGGATTGCCTGGAGTGAATGGTCTCTCAACTGAGCAGCGCAAGAGGCTAACGATTGCAGTAGAGCTTGTTGCCAATCCATCCATAATATTCATGGACGAGCCAACCTCTGGCCTTGATGCCAGGGCAGCTGCAATTGTAATGAGAACAGTGAGGAATACAGTGGACACCGGGCGAACTGTAGTCTGCACCATCCATCAGCCAAGCATTGACATATTTGATGCTTTTGATGAGCTGTTTCTTTTGAAAAGGGGAGGTGAAGAAATATATGTCGGTCCTTTAGGCCGTCATTCTGCCCATTTGATCAAGTACTTTGAGGATATTGATGGAGTTCCTAAAATTAAGGATGGTTACAATCCTGCAACTTGGATGCTAGAGATTACTGCAGCAGCACAAGAAGCAGTTCTTGGTGTTAACTTCACCGAAATATACAGGAACTCAGAACTATACAGGAGAAACAAGGCCATGATCAAGGAGCTAAGTACTCCTCCAGCCGGTTCAAAAGACTTGTACTTCCCTACTCAGTACTCTCAGTCTTTCTTCAGCCAGTGTATGGCCTGTCTATGGAAACAACATTTATCATACTGGCGAAACCCGCCATACAGTGCAGTGAGACTCTTGTTCACAACTTTCATAGCTTTAATGTTTGGGACAATATTCTGGGATCTGGGCTCGAAAAGGAGAAACCAACAAGATCTTTTCAATGCAATGGGTTCCATTTATGCTGCTGTTCTCTTCATTGGGTTTCAAAATTCCTCATCAGTACAGCCGGTTGTGGCTATTGAGAGGACAGTCTTTTACAGAGAAAGGGCAGCTGGAATGTACTCGGCATTGCCGTATGCCTTTGGACAGGTAGTGATTGAGCTCCCGTATATTTTTGTTCAGACAATCATATATGGAGTTATTGTGTACACCATGATCGGATTTGAGTGGACAGTCAGCAAGTTCCTTTGGTACATCTTCTTCATGTACTTCACTTTCTTATACTTCACATTCTATGGTATGATGACCGTAGCCGTGACTCCCAATCATAACATTGGTGCCATAGTCGCCTCTGCCTTCTATGCAATATGGAATCTCTTCTCAGGATTCATCGTTCCAAGAACGAGAATGCCAATTTGGTGGAGATGGTACTACTGGATTTGCCCGGTTTCATACACGTTATACGGATTGATTGCTTCGCAGTTTGGAGACATAAAAGACAAATTTGATTCAGGTGAAAGTGTGGAACACTTTGTCAGGAATTACTTTGGGTATAGACGGGACCTTTTGGGTGTAGTTGCAGCTGTGCACGTTGGCATTTGTGTGCTCTTTGGGTTCACTTTTGCCTTCTCAATCAAGGCATTTAATTTCCAAAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1452

Amino Acids

164.08

Weight (kDa)

6.78

Isoelectric Point (pI)

38.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 93 - 144 7.1e-07 ABC-transporter N-terminal
ABC_tran PF00005 169 - 350 1.3e-14 ABC transporter
ABC2_membrane PF01061 505 - 717 8.4e-50 ABC-2 type transporter
PDR_assoc PF08370 722 - 784 9.7e-28 Plant PDR ABC transporter associated
ABC_tran PF00005 882 - 1034 1.3e-19 ABC transporter
ABC2_membrane PF01061 1180 - 1393 1e-63 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000493)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29820 FvH4_2g29820 FvH4_2g29822 FvH4_2g29824 FvH4_2g29824 FvH4_2g29824 FvH4_2g29840 FvH4_2g29840 FvH4_2g29840 FvH4_5g00550 FvH4_5g00550
malus_domestica MD02G1032700.v1.1 MD08G1140900.v1.1 MD08G1141400.v1.1 MD15G1117800.v1.1 MD15G1117900.v1.1
prunus_persica Prupe.1G471200_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471400_v2.0.a1 Prupe.7G242900_v2.0.a1
pyrus_communis pycom02g02690 pycom08g11890
rosa_chinensis RchiOBHm_Chr2g0111741 RchiOBHm_Chr2g0145991 RchiOBHm_Chr6g0298851 RchiOBHm_Chr6g0298871 RchiOBHm_Chr6g0298891 RchiOBHm_Chr6g0298901 RchiOBHm_Chr6g0298911 RchiOBHm_Chr7g0201801 RchiOBHm_Chr7g0211531 RchiOBHm_Chr7g0214601
rosa_laevigata RLG00000002931 RLG00000003000 RLG00000003647 RLG00000011476 RLG00000011479
rosa_multiflora Rmu_sc0001030.1_g000012 Rmu_sc0001548.1_g000041 Rmu_ssc0000397.1_g000033 Rmu_ssc0000397.1_g000036 Rmu_ssc0000397.1_g000037 Rmu_ssc0000397.1_g000039 Rmu_ssc0000397.1_g000048
rosa_roxburghii Rroxscaffold_1G00003830 Rroxscaffold_3G00247810 Rroxscaffold_3G00255060 Rroxscaffold_7G00169220 Rroxscaffold_7G00169230 Rroxscaffold_7G00169280
rosa_rugosa Rorug02G0223600 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283200 Rorug06G0283300 Rorug06G0283400 Rorug06G0283500 Rorug07G0066000
rosa_samantha Rh2AG511300 Rh2CG235100 Rh2CG496400 Rh2DG330900 Rh6BG403100 Rh6BG403300 Rh6BG403400 Rh6BG403500 Rh6CG408700 Rh6CG408900 Rh6CG409000 Rh6CG409200 Rh6DG395800 Rh6DG395900 Rh6DG396000 Rh6DG396200 Rh7AG193400 Rh7BG195100 Rh7BG436600 Rh7DG199300 Rh7DG265500
rosa_wichuraiana Rw2G016900 Rw6G034570 Rw6G034580 Rw6G034590 Rw6G034600 Rw7G016900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 222
Acc16I TGCGCA 2 cut(s) 167, 937
Acc65I GGTACC 1 cut(s) 2924
AccB1I GGYRCC 4 cut(s) 453, 1156, 2924, 4031
AccB7I CCANNNNNTGG 3 cut(s) 569, 3101, 3544
AccI GTMKAC 1 cut(s) 1569
AccII CGCG 4 cut(s) 177, 1230, 2024, 2223
AccIII TCCGGA 1 cut(s) 1629
AciI CCGC 8 cut(s) 177, 494, 753, 981, 1228, 1343, 2223, 3587
AclWI GGATC 7 cut(s) 830, 1813, 1822, 1826, 1886, 2637, 3663
AcoI YGGCCR 1 cut(s) 2122
AcsI RAATTY 9 cut(s) 180, 460, 623, 1382, 1538, 1558, 2147, 3742, 4196
AcuI CTGAAG 4 cut(s) 1410, 2526, 2666, 3517
AcyI GRCGYC 1 cut(s) 219
AfeI AGCGCT 1 cut(s) 2988
AfiI CCNNNNNNNGG 7 cut(s) 569, 718, 1972, 3101, 3485, 3544, 3555
AflII CTTAAG 1 cut(s) 1823
AflIII ACRYGT 1 cut(s) 4149
AgeI ACCGGT 1 cut(s) 2657
AhlI ACTAGT 1 cut(s) 2038
AjnI CCWGG 4 cut(s) 562, 2598, 3001, 3113
AjuI GAANNNNNNNTTGG 6 cut(s) 2976, 3008, 3381, 3413, 4093, 4125
AloI GAACNNNNNNTCC 2 cut(s) 4071, 4103
Alw21I GWGCWC 6 cut(s) 43, 1036, 1257, 3858, 4284, 4302
Alw26I GTCTC 9 cut(s) 878, 2061, 2067, 2151, 2223, 2470, 3020, 3598, 4176
Alw44I GTGCAC 1 cut(s) 4280
AlwI GGATC 7 cut(s) 830, 1813, 1822, 1826, 1886, 2637, 3663
AlwNI CAGNNNCTG 2 cut(s) 569, 839
Ama87I CYCGRG 2 cut(s) 1241, 2064
Aor13HI TCCGGA 1 cut(s) 1629
Aor51HI AGCGCT 1 cut(s) 2988
AoxI GGCC 9 cut(s) 2103, 2122, 2310, 2364, 2669, 3103, 3274, 3453, 3546
ApaLI GTGCAC 1 cut(s) 4280
ApoI RAATTY 9 cut(s) 180, 460, 623, 1382, 1538, 1558, 2147, 3742, 4196
ArsI GACNNNNNNTTYG 2 cut(s) 915, 947
AseI ATTAAT 1 cut(s) 324
AsiGI ACCGGT 1 cut(s) 2657
Asp700I GAANNNNTTC 5 cut(s) 1236, 1395, 1562, 3393, 4070
Asp718I GGTACC 1 cut(s) 2924
AspLEI GCGC 6 cut(s) 168, 177, 938, 2708, 2989, 3033
AsuC2I CCSGG 3 cut(s) 2673, 3163, 4139
AsuHPI GGTGA 9 cut(s) 29, 178, 280, 1439, 1457, 2100, 3260, 3403, 4220
AsuII TTCGAA 1 cut(s) 2547
AvaI CYCGRG 2 cut(s) 1241, 2064
AvaII GGWCC 9 cut(s) 316, 557, 681, 773, 1022, 1223, 1774, 3266, 4255
AxyI CCTNAGG 1 cut(s) 2910
BaeGI GKGCMC 1 cut(s) 4284
BalI TGGCCA 1 cut(s) 2124
BamHI GGATCC 1 cut(s) 1818
BanI GGYRCC 4 cut(s) 453, 1156, 2924, 4031
BanII GRGCYC 2 cut(s) 3665, 3858
BarI GAAGNNNNNNTAC 2 cut(s) 3938, 3970
BbsI GAAGAC 2 cut(s) 90, 291
Bbv12I GWGCWC 6 cut(s) 43, 1036, 1257, 3858, 4284, 4302
BceAI ACGGC 4 cut(s) 1838, 3261, 3811, 3992
BciT130I CCWGG 4 cut(s) 564, 2600, 3003, 3115
BciVI GTATCC 1 cut(s) 2799
BclI TGATCA 4 cut(s) 1698, 2386, 3294, 3459
BcnI CCSGG 3 cut(s) 2673, 3163, 4139
BcoDI GTCTC 9 cut(s) 878, 2061, 2067, 2151, 2223, 2470, 3020, 3598, 4176
BcuI ACTAGT 1 cut(s) 2038
BfaI CTAG 3 cut(s) 534, 2039, 3365
BfmI CTRYAG 5 cut(s) 276, 1470, 2268, 3171, 3375
BfoI RGCGCY 2 cut(s) 2709, 2990
BfrI CTTAAG 1 cut(s) 1823
BfuI GTATCC 1 cut(s) 2799
BglII AGATCT 2 cut(s) 616, 3685
BmcAI AGTACT 6 cut(s) 1337, 2037, 2352, 3302, 3475, 3518
Bme1390I CCNGG 7 cut(s) 564, 2600, 2673, 3003, 3115, 3163, 4139
Bme18I GGWCC 9 cut(s) 316, 557, 681, 773, 1022, 1223, 1774, 3266, 4255
BmeT110I CYCGRG 2 cut(s) 1241, 2064
BmrFI CCNGG 7 cut(s) 564, 2600, 2673, 3003, 3115, 3163, 4139
BmrI ACTGGG 2 cut(s) 1348, 2403
BmsI GCATC 9 cut(s) 499, 875, 1002, 1629, 1961, 2900, 3100, 3202, 3351
BmuI ACTGGG 2 cut(s) 1348, 2403
BpiI GAAGAC 2 cut(s) 90, 291
BplI GAGNNNNNCTC 6 cut(s) 25, 57, 668, 700, 3083, 3115
BpmI CTGGAG 3 cut(s) 278, 3024, 3465
Bpu14I TTCGAA 1 cut(s) 2547
BpuEI CTTGAG 5 cut(s) 296, 896, 947, 2328, 2598
BpuMI CCSGG 3 cut(s) 2673, 3163, 4139
BsaBI GATNNNNATC 1 cut(s) 1832
BsaHI GRCGYC 1 cut(s) 219
BsaI GGTCTC 2 cut(s) 878, 3020
BsaWI WCCGGW 5 cut(s) 318, 1629, 2657, 2898, 2921
BsaXI ACNNNNNCTCC 6 cut(s) 1611, 1641, 2256, 2286, 3882, 3912
Bsc4I CCNNNNNNNGG 7 cut(s) 569, 718, 1972, 3101, 3485, 3544, 3555
Bse118I RCCGGY 5 cut(s) 1024, 1153, 2657, 3485, 3760
Bse1I ACTGG 9 cut(s) 747, 888, 1063, 1343, 2255, 2398, 3538, 3581, 4133
Bse21I CCTNAGG 1 cut(s) 2910
Bse3DI GCAATG 2 cut(s) 3705, 3821
Bse8I GATNNNNATC 1 cut(s) 1832
BseAI TCCGGA 1 cut(s) 1629
BseBI CCWGG 4 cut(s) 564, 2600, 3003, 3115
BseJI GATNNNNATC 1 cut(s) 1832
BseLI CCNNNNNNNGG 7 cut(s) 569, 718, 1972, 3101, 3485, 3544, 3555
BseMI GCAATG 2 cut(s) 3705, 3821
BseNI ACTGG 9 cut(s) 747, 888, 1063, 1343, 2255, 2398, 3538, 3581, 4133
BseRI GAGGAG 2 cut(s) 2844, 3468
BseSI GKGCMC 1 cut(s) 4284
BsgI GTGCAG 2 cut(s) 3163, 3618
Bsh1236I CGCG 4 cut(s) 177, 1230, 2024, 2223
BshFI GGCC 9 cut(s) 2105, 2124, 2312, 2366, 2671, 3105, 3276, 3455, 3548
BshNI GGYRCC 4 cut(s) 453, 1156, 2924, 4031
BshTI ACCGGT 1 cut(s) 2657
BsiHKAI GWGCWC 6 cut(s) 43, 1036, 1257, 3858, 4284, 4302
BsiHKCI CYCGRG 2 cut(s) 1241, 2064
BslFI GGGAC 6 cut(s) 936, 968, 1209, 1787, 3655, 4268
BslI CCNNNNNNNGG 7 cut(s) 569, 718, 1972, 3101, 3485, 3544, 3555
BsmAI GTCTC 9 cut(s) 878, 2061, 2067, 2151, 2223, 2470, 3020, 3598, 4176
BsmBI CGTCTC 1 cut(s) 2061
BsmFI GGGAC 6 cut(s) 936, 968, 1209, 1787, 3655, 4268
BsmI GAATGC 2 cut(s) 168, 4107
BsnI GGCC 9 cut(s) 2105, 2124, 2312, 2366, 2671, 3105, 3276, 3455, 3548
Bso31I GGTCTC 2 cut(s) 878, 3020
BsoBI CYCGRG 2 cut(s) 1241, 2064
Bsp119I TTCGAA 1 cut(s) 2547
Bsp1286I GDGCHC 7 cut(s) 43, 1036, 1257, 3665, 3858, 4284, 4302
Bsp13I TCCGGA 1 cut(s) 1629
Bsp1407I TGTACA 1 cut(s) 3900
Bsp19I CCATGG 1 cut(s) 78
BspACI CCGC 8 cut(s) 177, 494, 753, 981, 1228, 1343, 2223, 3587
BspANI GGCC 9 cut(s) 2105, 2124, 2312, 2366, 2671, 3105, 3276, 3455, 3548
BspEI TCCGGA 1 cut(s) 1629
BspFNI CGCG 4 cut(s) 177, 1230, 2024, 2223
BspHI TCATGA 2 cut(s) 1743, 1866
BspMAI CTGCAG 3 cut(s) 280, 1474, 3379
BspPI GGATC 7 cut(s) 830, 1813, 1822, 1826, 1886, 2637, 3663
BspQI GCTCTTC 1 cut(s) 1347
BspT104I TTCGAA 1 cut(s) 2547
BspT107I GGYRCC 4 cut(s) 453, 1156, 2924, 4031
BspTI CTTAAG 1 cut(s) 1823
BspTNI GGTCTC 2 cut(s) 878, 3020
BsrDI GCAATG 2 cut(s) 3705, 3821
BsrFI RCCGGY 5 cut(s) 1024, 1153, 2657, 3485, 3760
BsrGI TGTACA 1 cut(s) 3900
BsrI ACTGG 9 cut(s) 747, 888, 1063, 1343, 2255, 2398, 3538, 3581, 4133
BssAI RCCGGY 5 cut(s) 1024, 1153, 2657, 3485, 3760
BssNI GRCGYC 1 cut(s) 219
BssT1I CCWWGG 5 cut(s) 78, 1219, 1377, 1458, 2367
Bst2UI CCWGG 4 cut(s) 564, 2600, 3003, 3115
Bst6I CTCTTC 9 cut(s) 202, 657, 1149, 1347, 1547, 1787, 2437, 3731, 4076
BstACI GRCGYC 1 cut(s) 219
BstAFI CTTAAG 1 cut(s) 1823
BstAUI TGTACA 1 cut(s) 3900
BstBI TTCGAA 1 cut(s) 2547
BstC8I GCNNGC 5 cut(s) 1437, 1525, 1731, 2031, 2815
BstDSI CCRYGG 2 cut(s) 78, 2441
BstEII GGTNACC 1 cut(s) 1463
BstFNI CGCG 4 cut(s) 177, 1230, 2024, 2223
BstH2I RGCGCY 2 cut(s) 2709, 2990
BstHHI GCGC 6 cut(s) 168, 177, 938, 2708, 2989, 3033
BstMAI GTCTC 9 cut(s) 878, 2061, 2067, 2151, 2223, 2470, 3020, 3598, 4176
BstNI CCWGG 4 cut(s) 564, 2600, 3003, 3115
BstNSI RCATGY 1 cut(s) 2596
BstPI GGTNACC 1 cut(s) 1463
BstSCI CCNGG 7 cut(s) 562, 2598, 2671, 3001, 3113, 3161, 4137
BstSFI CTRYAG 5 cut(s) 276, 1470, 2268, 3171, 3375
BstSLI GKGCMC 1 cut(s) 4284
BstUI CGCG 4 cut(s) 177, 1230, 2024, 2223
BstV2I GAAGAC 2 cut(s) 90, 291
BstX2I RGATCY 6 cut(s) 616, 835, 1818, 1827, 3655, 3685
BstXI CCANNNNNNTGG 1 cut(s) 4113
BstYI RGATCY 6 cut(s) 616, 835, 1818, 1827, 3655, 3685
Bsu36I CCTNAGG 1 cut(s) 2910
BsuI GTATCC 1 cut(s) 2799
BsuRI GGCC 9 cut(s) 2105, 2124, 2312, 2366, 2671, 3105, 3276, 3455, 3548
BtgI CCRYGG 2 cut(s) 78, 2441
BtgZI GCGATG 1 cut(s) 852
BtsI GCAGTG 1 cut(s) 3606
Cac8I GCNNGC 5 cut(s) 1437, 1525, 1731, 2031, 2815
CaiI CAGNNNCTG 2 cut(s) 569, 839
CciI TCATGA 2 cut(s) 1743, 1866
CfoI GCGC 6 cut(s) 168, 177, 938, 2708, 2989, 3033
Cfr10I RCCGGY 5 cut(s) 1024, 1153, 2657, 3485, 3760
CseI GACGC 1 cut(s) 553
CspAI ACCGGT 1 cut(s) 2657
DraI TTTAAA 1 cut(s) 375
EaeI YGGCCR 1 cut(s) 2122
Eam1104I CTCTTC 9 cut(s) 202, 657, 1149, 1347, 1547, 1787, 2437, 3731, 4076
EarI CTCTTC 9 cut(s) 202, 657, 1149, 1347, 1547, 1787, 2437, 3731, 4076
EciI GGCGGA 2 cut(s) 996, 1358
Ecl136II GAGCTC 1 cut(s) 3856
Eco130I CCWWGG 5 cut(s) 78, 1219, 1377, 1458, 2367
Eco147I AGGCCT 1 cut(s) 2366
Eco24I GRGCYC 2 cut(s) 3665, 3858
Eco31I GGTCTC 2 cut(s) 878, 3020
Eco32I GATATC 2 cut(s) 850, 1053
Eco47I GGWCC 9 cut(s) 316, 557, 681, 773, 1022, 1223, 1774, 3266, 4255
Eco47III AGCGCT 1 cut(s) 2988
Eco53kI GAGCTC 1 cut(s) 3856
Eco57I CTGAAG 4 cut(s) 1410, 2526, 2666, 3517
Eco81I CCTNAGG 1 cut(s) 2910
Eco88I CYCGRG 2 cut(s) 1241, 2064
Eco91I GGTNACC 1 cut(s) 1463
EcoICRI GAGCTC 1 cut(s) 3856
EcoO109I RGGNCCY 5 cut(s) 557, 681, 1223, 1774, 4255
EcoO65I GGTNACC 1 cut(s) 1463
EcoRI GAATTC 2 cut(s) 180, 1558
EcoRII CCWGG 4 cut(s) 562, 2598, 3001, 3113
EcoRV GATATC 2 cut(s) 850, 1053
EcoT14I CCWWGG 5 cut(s) 78, 1219, 1377, 1458, 2367
EcoT22I ATGCAT 1 cut(s) 1644
EcoT38I GRGCYC 2 cut(s) 3665, 3858
ErhI CCWWGG 5 cut(s) 78, 1219, 1377, 1458, 2367
Esp3I CGTCTC 1 cut(s) 2061
FalI AAGNNNNNCTT 2 cut(s) 3381, 3413
FaqI GGGAC 6 cut(s) 936, 968, 1209, 1787, 3655, 4268
FauI CCCGC 3 cut(s) 1235, 2216, 3594
FbaI TGATCA 4 cut(s) 1698, 2386, 3294, 3459
FblI GTMKAC 1 cut(s) 1569
FriOI GRGCYC 2 cut(s) 3665, 3858
FspBI CTAG 3 cut(s) 534, 2039, 3365
FspI TGCGCA 2 cut(s) 167, 937
GlaI GCGC 6 cut(s) 167, 176, 937, 2707, 2988, 3032
GsuI CTGGAG 3 cut(s) 278, 3024, 3465
HaeII RGCGCY 2 cut(s) 2709, 2990
HaeIII GGCC 9 cut(s) 2105, 2124, 2312, 2366, 2671, 3105, 3276, 3455, 3548
HgaI GACGC 1 cut(s) 553
HhaI GCGC 6 cut(s) 168, 177, 938, 2708, 2989, 3033
Hin1I GRCGYC 1 cut(s) 219
Hin6I GCGC 6 cut(s) 166, 175, 936, 2706, 2987, 3031
HinP1I GCGC 6 cut(s) 166, 175, 936, 2706, 2987, 3031
HincII GTYRAC 3 cut(s) 1657, 2590, 3406
HindII GTYRAC 3 cut(s) 1657, 2590, 3406
HindIII AAGCTT 2 cut(s) 390, 1521
HpaI GTTAAC 1 cut(s) 3406
HphI GGTGA 9 cut(s) 29, 178, 280, 1439, 1457, 2100, 3260, 3403, 4220
HpyAV CCTTC 9 cut(s) 191, 853, 869, 1501, 1787, 2641, 2759, 4060, 4330
HpyCH4IV ACGT 5 cut(s) 219, 778, 1871, 4151, 4284
HpySE526I ACGT 5 cut(s) 219, 778, 1871, 4151, 4284
Hsp92I GRCGYC 1 cut(s) 219
HspAI GCGC 6 cut(s) 166, 175, 936, 2706, 2987, 3031
Kpn2I TCCGGA 1 cut(s) 1629
KpnI GGTACC 1 cut(s) 2928
Ksp22I TGATCA 4 cut(s) 1698, 2386, 3294, 3459
KspAI GTTAAC 1 cut(s) 3406
LguI GCTCTTC 1 cut(s) 1347
LmnI GCTCC 7 cut(s) 1330, 1935, 2166, 2264, 2956, 3466, 3861
LweI GCATC 9 cut(s) 499, 875, 1002, 1629, 1961, 2900, 3100, 3202, 3351
MaeI CTAG 3 cut(s) 534, 2039, 3365
MaeII ACGT 5 cut(s) 219, 778, 1871, 4151, 4284
MaeIII GTNAC 9 cut(s) 895, 997, 1309, 1463, 2620, 2653, 2860, 3341, 4009
MfeI CAATTG 2 cut(s) 351, 3126
MflI RGATCY 6 cut(s) 616, 835, 1818, 1827, 3655, 3685
MhlI GDGCHC 7 cut(s) 43, 1036, 1257, 3665, 3858, 4284, 4302
MlsI TGGCCA 1 cut(s) 2124
MluNI TGGCCA 1 cut(s) 2124
MlyI GAGTC 6 cut(s) 1005, 1579, 2685, 2882, 3600, 4006
MmeI TCCRAC 7 cut(s) 313, 751, 1000, 1174, 2144, 2254, 2974
Mox20I TGGCCA 1 cut(s) 2124
Mph1103I ATGCAT 1 cut(s) 1644
MroI TCCGGA 1 cut(s) 1629
MroXI GAANNNNTTC 5 cut(s) 1236, 1395, 1562, 3393, 4070
MscI TGGCCA 1 cut(s) 2124
MslI CAYNNNNRTG 5 cut(s) 375, 947, 1190, 1706, 4295
Msp20I TGGCCA 1 cut(s) 2124
MspA1I CMGCKG 3 cut(s) 3122, 3806, 4277
MspCI CTTAAG 1 cut(s) 1823
MspR9I CCNGG 7 cut(s) 564, 2600, 2673, 3003, 3115, 3163, 4139
MunI CAATTG 2 cut(s) 351, 3126
Mva1269I GAATGC 2 cut(s) 168, 4107
MvaI CCWGG 4 cut(s) 564, 2600, 3003, 3115
MvnI CGCG 4 cut(s) 177, 1230, 2024, 2223
NciI CCSGG 3 cut(s) 2673, 3163, 4139
NcoI CCATGG 1 cut(s) 78
NmeAIII GCCGAG 2 cut(s) 665, 3797
NmuCI GTSAC 4 cut(s) 997, 1463, 2653, 4009
NsbI TGCGCA 2 cut(s) 167, 937
NsiI ATGCAT 1 cut(s) 1644
NspI RCATGY 1 cut(s) 2596
NspV TTCGAA 1 cut(s) 2547
PaeR7I CTCGAG 2 cut(s) 1241, 2064
PagI TCATGA 2 cut(s) 1743, 1866
PceI AGGCCT 1 cut(s) 2366
PciSI GCTCTTC 1 cut(s) 1347
PcsI WCGNNNNNNNCGW 1 cut(s) 3824
PctI GAATGC 2 cut(s) 168, 4107
PdmI GAANNNNTTC 5 cut(s) 1236, 1395, 1562, 3393, 4070
PfeI GAWTC 9 cut(s) 226, 1106, 1263, 2192, 2399, 2918, 4066, 4080, 4202
PflMI CCANNNNNTGG 3 cut(s) 569, 3101, 3544
PinAI ACCGGT 1 cut(s) 2657
PleI GAGTC 6 cut(s) 1004, 1579, 2684, 2881, 3600, 4006
PpsI GAGTC 6 cut(s) 1004, 1579, 2684, 2881, 3600, 4006
PpuMI RGGWCCY 5 cut(s) 557, 681, 1223, 1774, 4255
PshBI ATTAAT 1 cut(s) 324
Psp124BI GAGCTC 1 cut(s) 3858
Psp5II RGGWCCY 5 cut(s) 557, 681, 1223, 1774, 4255
Psp6I CCWGG 4 cut(s) 562, 2598, 3001, 3113
PspEI GGTNACC 1 cut(s) 1463
PspGI CCWGG 4 cut(s) 562, 2598, 3001, 3113
PspPPI RGGWCCY 5 cut(s) 557, 681, 1223, 1774, 4255
PstI CTGCAG 3 cut(s) 280, 1474, 3379
PstNI CAGNNNCTG 2 cut(s) 569, 839
PsuI RGATCY 6 cut(s) 616, 835, 1818, 1827, 3655, 3685
PvuII CAGCTG 3 cut(s) 3122, 3806, 4277
RseI CAYNNNNRTG 5 cut(s) 375, 947, 1190, 1706, 4295
SacI GAGCTC 1 cut(s) 3858
SapI GCTCTTC 1 cut(s) 1347
ScaI AGTACT 6 cut(s) 1337, 2037, 2352, 3302, 3475, 3518
SchI GAGTC 6 cut(s) 1005, 1579, 2685, 2882, 3600, 4006
ScrFI CCNGG 7 cut(s) 564, 2600, 2673, 3003, 3115, 3163, 4139
SduI GDGCHC 7 cut(s) 43, 1036, 1257, 3665, 3858, 4284, 4302
SfaNI GCATC 9 cut(s) 499, 875, 1002, 1629, 1961, 2900, 3100, 3202, 3351
SfcI CTRYAG 5 cut(s) 276, 1470, 2268, 3171, 3375
Sfr274I CTCGAG 2 cut(s) 1241, 2064
SfuI TTCGAA 1 cut(s) 2547
SinI GGWCC 9 cut(s) 316, 557, 681, 773, 1022, 1223, 1774, 3266, 4255
SlaI CTCGAG 2 cut(s) 1241, 2064
SmiI ATTTAAAT 1 cut(s) 375
SmiMI CAYNNNNRTG 5 cut(s) 375, 947, 1190, 1706, 4295
SmlI CTYRAG 8 cut(s) 311, 911, 926, 1241, 1823, 2064, 2343, 2613
SmoI CTYRAG 8 cut(s) 311, 911, 926, 1241, 1823, 2064, 2343, 2613
SpeI ACTAGT 1 cut(s) 2038
SseBI AGGCCT 1 cut(s) 2366
SsiI CCGC 8 cut(s) 177, 494, 753, 981, 1228, 1343, 2223, 3587
SspI AATATT 5 cut(s) 61, 423, 442, 3081, 3648
SspMI CTAG 3 cut(s) 534, 2039, 3365
SstI GAGCTC 1 cut(s) 3858
StuI AGGCCT 1 cut(s) 2366
StyD4I CCNGG 7 cut(s) 562, 2598, 2671, 3001, 3113, 3161, 4137
StyI CCWWGG 5 cut(s) 78, 1219, 1377, 1458, 2367
SwaI ATTTAAAT 1 cut(s) 375
TaiI ACGT 5 cut(s) 222, 781, 1874, 4154, 4287
TaqI TCGA 8 cut(s) 1242, 1417, 1902, 2065, 2455, 2547, 2975, 3665
TaqII GACCGA 1 cut(s) 3254
TfiI GAWTC 9 cut(s) 226, 1106, 1263, 2192, 2399, 2918, 4066, 4080, 4202
TseFI GTSAC 4 cut(s) 997, 1463, 2653, 4009
Tsp45I GTSAC 4 cut(s) 997, 1463, 2653, 4009
TspGWI ACGGA 3 cut(s) 663, 2458, 4173
Van91I CCANNNNNTGG 3 cut(s) 569, 3101, 3544
Vha464I CTTAAG 1 cut(s) 1823
VneI GTGCAC 1 cut(s) 4280
VpaK11BI GGWCC 9 cut(s) 316, 557, 681, 773, 1022, 1223, 1774, 3266, 4255
VspI ATTAAT 1 cut(s) 324
XapI RAATTY 9 cut(s) 180, 460, 623, 1382, 1538, 1558, 2147, 3742, 4196
XceI RCATGY 1 cut(s) 2596
XcmI CCANNNNNNNNNTGG 5 cut(s) 586, 1121, 2247, 2365, 3082
XhoI CTCGAG 2 cut(s) 1241, 2064
XmiI GTMKAC 1 cut(s) 1569
XmnI GAANNNNTTC 5 cut(s) 1236, 1395, 1562, 3393, 4070
XspI CTAG 3 cut(s) 534, 2039, 3365
ZraI GACGTC 1 cut(s) 220
ZrmI AGTACT 6 cut(s) 1337, 2037, 2352, 3302, 3475, 3518
Zsp2I ATGCAT 1 cut(s) 1644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.