MD15G1117900.v1.1
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000493
Physical Location & Seq
Reverse (-)
8414359 .. 8421467
7109 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1117900.v1.1.491

Sequence Viewer

Length: 4356 bp
ATGGATAATGGTAATGGTGACATATTTAGAGTGAGTAGTGCTCGTTTGAGCAGTTCAAATATTTGGAGAAACAGCACCATGGATGTTTTCTCCAAGTCTTCACACGATGCAGATGATGAAGAAGCTCTCAAATGGGCAGCCATTGAGAAGCTGCCTACTTATTTGCGCATTCAGCGCGGCATTCTCACTGAGGAAGAAGGCAAAGGAAGAGAGATTGATATCAAGAATCTTGGGTTGCTTGAAAGAAAGAATGTGTTGGAGAGGCTGGTGAAAACTGCAGATGAAGATAATGAGAAGTTCTTGTTGAAGCTCAAGGACCGGATTAATCGCGTTGGACTTGATATTCCAACAATTGAATTGCGTTTTGAGCATTTAAATGTTGAAGCAGAAGCTTATGTGGGAGGCAGGGCTTTACCTACAATATTCAATTTTTTGGCTAATATTTTGGAGGGATGCCTGAATTTTGTTCACGTTCTTCCAAGTAGAAAGCATCCGTTACCGATCCTTGCCGATGTTAGTGGAATTATCAAGCCTAGAAGAATGACACTGCTTTTAGGACCCCCAAGCTCTGGAAAAACCACATTACTATTGGCTTTGGCTGGAAAACTTGCTAAAGATCTAAAATTTTCTGGGAGAGTTGAGTATAACGGACATGGGATGGAAGAGTTTGTGCCGGAGAGGACCTCGGCTTATATTAGTCAGCATGATCTCCACATAGGCGAAATGACAGTGAGAGAAACACTGGCTTTTTCGGCAAGATGTCAAGGGGTCGGACCACGTTACGAAATGTTGGCAGAATTATCAAGGAGAGAAAAGGAAGCGAACATTATACCAGATCCTGATCTAGATATCTACATGAAGGCAGCATCACTGCAAGGACAGGAGACCAATGTAGTTACAGATTATATACTCAAGATTTTGGGACTTGAGGTTTGCGCGGACATCATGGTGGGGGATGAAATGGTACGAGGTATTTCTGGCGGACAAAAGAAGCGACTCACAACAGGGGAGATGCTGGTCGGGCCGGCAAGAGCGCTTTTTATGGATGAGATATCAACCGGTTTAGACAGTTCAACAACATTTCAGATAGTGAAATCACTCAGAGAATCTATCCACATCCTCAGTGGAACTGCTGTTATCTCTCTTCTACAACCGGCACCAGAAACCTACGACTTATTTGATGACATAATTCTTCTGTCTGATGGACAAATTGTGTACCAAGGTCCCCGTGAAAATGTGCTTGAGTTCTTTGAGCACATGGGATTCAAATGTCCAGAGAGGAAAGGAGTAGCTGACTTCTTACAAGAAGTAACATCAAAGAAAGATCAGGAGCAGTACTGGGCGGATAAAGAAGAGCCATATAGTTTTATATCTTCCAAGGAATTTGCTGAAGCATTTCAGTCATTTCATATTGGTCGAAAACTTGGCGATGAGCTTGCTACTCCATTTGAAAAGTCCATGGGTCACCCCGCAGCTTTGACAACTCAGAAGTATGGTGTTAGCAAGAAGGAATTGCTTAAAGCTTGCATTGATAGAGAATTCTTGCTTATGAAGAGGAATTCGTTCGTCTACATTTTCAAAATGACTCAGCTCACTCTAATGGCTTTTATATCAATGACGCTATTTTGCCGGACTGACTTGCACAAGAATACAGTTGACGATGGGGGTATTTTCATGGGTGCTATGTTCTTCGCAGTGGTCATAATTATGTTTAATGGGTTCTCAGAGCTTGCCATGACTATCATGAAACTTCCTGTATTTTTCAAGCAAAGGGACCTTCTGTTCTATCCTGCTTGGGCATACTCTTTACCGACATGGATCCTTAAGATCCCTATCAGCTTCGTAGAAGTTGCAGTTTGGGTGATCATGACATACTATGTAATCGGTTTTGATCCAAATTTTGAAAGGTTTCTCAAACAATATCTTTTACTCCTATTGCTCAACCAGATGGCATCAGGACTCTTCCGATTTATGGGAGCATTAGGGAGGAATATAATTGTTGCAAACACATTCGGGTCTTTCGCCTTGGTTGCAGTACTTGTTTTGGGTGGCTTTGTCTTGTCTCGAGATAACGTGATGAAGTGGTGGGTATGGGGTTATTGGGCCTCGCCGATGATGTATGGCCAAAACGCTATAGCTGTCAATGAGTTTCTCGGAAACAGTTGGAGCCATGTTCCTCCTAATTCAACAGAATCGTTAGGAATCATGGTCTTGAAGTCTCGTGGGGTTTTCTTAGAACCGTATTGGTACTGGCTTGGAGTAGGAGCTACAATCGGTTATATTTTCCTATTCAATTTCTTCTACACTTTGGCCTTGAAGTTTCTTAACCCATTTGGGAAGCCTCAAGCAGTACTATCCAAAGAGGCCTTGGCTGAGAAACTTGCCGATAAAGCTGGAGATTCCGTTGAGCTATCATCAAGAGGAAAGAACTCTTCTGACTCAAGGAATGAAAGTCGAAGAAGCGTATCATCCAGAACACTGTCTGCGAGAGTGGGCAGCATTGCTGAAGTCAATGGAAACAAGAAGCGAGGAATGGTTCTGCCTTTTGAACCCCTTTGGATTACTTTTGATGAAATCAAATATGCCGTTGACATGCCCCAGGAAATGAAAACTCAAGGCGTAACTGAGGATCGACTACAACTCCTGAAGGGTGTGACCGGTGCTTTTAGGCCGGGAGTCTTAACAGCTCTAATGGGTATTAGTGGCGCTGGAAAGACTACTCTAATGGATGTGTTGGCAGGGAGGAAAATGGGGGGATATATCGAAGGAAACATTACAATATCTGGGTATCCAAAGAAGCAAGAAACATTTGCTCGCATATCAGGATATTGTGAGCAAACCGATATACACTCTCCTCATGTTACAGTTTATGAGTCTTTGGTTTACTCTGCATCCCTCCGGTTGCCTCCTGAGGTTGATTCCACAACCAGAAAGATGTTTATTGAGGAAGTCATGGAGCTTGTGGAATTGACCTCGATAAGGGAAGCACTTGTTGGATTGCCTGGAGTGAATGGTCTCTCAACTGAGCAGCGTAAAAGGCTAACGATTGCAGTAGAGCTTGTTGCCAATCCATCCATAATATTTATGGACGAGCCAACCTCTGGCCTTGATGCCAGGGCAGCTGCAATTGTAATGAGAACGGTGAGGAATACAGTGGACACCGGGCGAACTGTAGTCTGCACCATCCATCAGCCAAGCATTGACATATTTGATGCTTTCGATGAACTGTTTCTTTTGAAAAGAGGAGGTGAAGAAATATTTGTCGGTCCTTTAGGTCGCCATTCTTCCCATTTGATCAAGTACTTTGAGGATATCGATGGAGTTCCCAAAATTAAGGATGGTTACAATCCTGCAACTTGGATGCTAGAGATTACTGCAGCAGCACAAGAAGCAAGTATTGGTGTTAACTTCACCGAAATATATAGGAACTCAGAACTATACGGGAGAAACAAGGCCATGATCAAGGAGCTAAGCACTCCTCCAGCAGGTTCAAAAGACTTGTACTTCCCTACTCAGTACTCTCAGTCTTTCTTCAGCCAGTGTATGGCCTGTCTATGGAAACAACATTTATCATACTGGCGAAACCCGCCGTACAGTGCAGTGAGACTCATGTTCACAACTTTCATAGCTTTAATGTTCGGAACAATATTTTGGGATCTGGGCTCAAAAAGGACAAGCAAACAAGATCTTTTCAATGCAATGGGTTCCATGTATGCTGCTGTTCTCTTCATTGGGGTACAAAATGCCGCATCAGTACAGCCGGTTGTGGCTATTGAGAGGACAGTCTTTTACAGAGAGAGGGCGGCTGGAATGTACTCGGCATTGCCATATGCCTTTGGACAGGTAGTGATTGAGCTCCCGTATATTTTTGTTCAGACAATCATATACGGAGTTATTGTATATGCCATGATCGGATTTGACTGGACAGTCAGCAAGTTCCTTTGGTATCTATTCTTCATGTACTTCACTTTCTTATACTTCACATTCTATGGTATGATGACCGTTGCCGTGACTCCCAATCACAACATTGCTGCTATAGTCTCCTCTGCCTTCTATGCAATATGGAATCTCTTCTCAGGATTCATCGTTCCAAGAACAAGAATGCCAATATGGTGGAGATGGTACTATTGGATATGCCCAGTTTCATACACGTTGTACGGATTGGTTGCTTCGCAGTTTGGAGACATAAAAGACAAGTTCGATACAGGTGAAAGTGTGGAACACTTTGTGAGGGATTACTTTGGGTACAGACGGGACTTTTTGGGTGCAGTTGCAGCTGTGCATGTTGGCATTTGTGTGCTCTTTGGCTTCACTTTTGCCTTCTCAATCAAGGTATTTAATTTCCAAAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1452

Amino Acids

163.73

Weight (kDa)

6.78

Isoelectric Point (pI)

36.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 93 - 144 7.4e-06 ABC-transporter N-terminal
ABC_tran PF00005 170 - 350 1.1e-12 ABC transporter
ABC2_membrane_7 PF19055 384 - 436 5.4e-06 ABC-2 type transporter
ABC2_membrane PF01061 505 - 717 2e-49 ABC-2 type transporter
PDR_assoc PF08370 722 - 784 3.8e-27 Plant PDR ABC transporter associated
ABC_tran PF00005 882 - 1034 1.3e-19 ABC transporter
ABC2_membrane PF01061 1180 - 1393 2.3e-63 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000493)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29820 FvH4_2g29820 FvH4_2g29822 FvH4_2g29824 FvH4_2g29824 FvH4_2g29824 FvH4_2g29840 FvH4_2g29840 FvH4_2g29840 FvH4_5g00550 FvH4_5g00550
malus_domestica MD02G1032700.v1.1 MD08G1140900.v1.1 MD08G1141400.v1.1 MD15G1117800.v1.1 MD15G1117900.v1.1
prunus_persica Prupe.1G471200_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471400_v2.0.a1 Prupe.7G242900_v2.0.a1
pyrus_communis pycom02g02690 pycom08g11890
rosa_chinensis RchiOBHm_Chr2g0111741 RchiOBHm_Chr2g0145991 RchiOBHm_Chr6g0298851 RchiOBHm_Chr6g0298871 RchiOBHm_Chr6g0298891 RchiOBHm_Chr6g0298901 RchiOBHm_Chr6g0298911 RchiOBHm_Chr7g0201801 RchiOBHm_Chr7g0211531 RchiOBHm_Chr7g0214601
rosa_laevigata RLG00000002931 RLG00000003000 RLG00000003647 RLG00000011476 RLG00000011479
rosa_multiflora Rmu_sc0001030.1_g000012 Rmu_sc0001548.1_g000041 Rmu_ssc0000397.1_g000033 Rmu_ssc0000397.1_g000036 Rmu_ssc0000397.1_g000037 Rmu_ssc0000397.1_g000039 Rmu_ssc0000397.1_g000048
rosa_roxburghii Rroxscaffold_1G00003830 Rroxscaffold_3G00247810 Rroxscaffold_3G00255060 Rroxscaffold_7G00169220 Rroxscaffold_7G00169230 Rroxscaffold_7G00169280
rosa_rugosa Rorug02G0223600 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283200 Rorug06G0283300 Rorug06G0283400 Rorug06G0283500 Rorug07G0066000
rosa_samantha Rh2AG511300 Rh2CG235100 Rh2CG496400 Rh2DG330900 Rh6BG403100 Rh6BG403300 Rh6BG403400 Rh6BG403500 Rh6CG408700 Rh6CG408900 Rh6CG409000 Rh6CG409200 Rh6DG395800 Rh6DG395900 Rh6DG396000 Rh6DG396200 Rh7AG193400 Rh7BG195100 Rh7BG436600 Rh7DG199300 Rh7DG265500
rosa_wichuraiana Rw2G016900 Rw6G034570 Rw6G034580 Rw6G034590 Rw6G034600 Rw7G016900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 3926
Acc16I TGCGCA 1 cut(s) 167
Acc36I ACCTGC 1 cut(s) 3476
AccB1I GGYRCC 1 cut(s) 1156
AccB7I CCANNNNNTGG 3 cut(s) 569, 3101, 3544
AccI GTMKAC 1 cut(s) 1569
AccII CGCG 3 cut(s) 177, 330, 938
AciI CCGC 8 cut(s) 177, 938, 981, 1343, 1470, 3587, 3747, 3803
AclWI GGATC 8 cut(s) 496, 830, 1813, 1822, 1826, 1886, 2637, 3663
AcoI YGGCCR 1 cut(s) 2122
AcsI RAATTY 6 cut(s) 460, 623, 1382, 1538, 1558, 1897
AcuI CTGAAG 4 cut(s) 1410, 2526, 2666, 3517
AdeI CACNNNGTG 1 cut(s) 4228
AfeI AGCGCT 1 cut(s) 1035
AfiI CCNNNNNNNGG 7 cut(s) 569, 1972, 2979, 3101, 3485, 3544, 3555
AflII CTTAAG 1 cut(s) 1823
AflIII ACRYGT 1 cut(s) 4149
AgeI ACCGGT 2 cut(s) 1058, 2657
AjnI CCWGG 3 cut(s) 2598, 3001, 3113
AjuI GAANNNNNNNTTGG 6 cut(s) 2976, 3008, 3381, 3413, 3634, 3666
AloI GAACNNNNNNTCC 4 cut(s) 1766, 1798, 4071, 4103
Alw21I GWGCWC 4 cut(s) 43, 1257, 3858, 4302
Alw26I GTCTC 7 cut(s) 878, 2067, 2223, 3020, 3598, 4045, 4176
AlwI GGATC 8 cut(s) 496, 830, 1813, 1822, 1826, 1886, 2637, 3663
AlwNI CAGNNNCTG 1 cut(s) 839
Ama87I CYCGRG 1 cut(s) 2064
Aor51HI AGCGCT 1 cut(s) 1035
AoxI GGCC 9 cut(s) 1022, 2103, 2122, 2310, 2364, 2669, 3103, 3453, 3546
ApoI RAATTY 6 cut(s) 460, 623, 1382, 1538, 1558, 1897
ArsI GACNNNNNNTTYG 2 cut(s) 915, 947
AseI ATTAAT 1 cut(s) 324
AsiGI ACCGGT 2 cut(s) 1058, 2657
Asp700I GAANNNNTTC 5 cut(s) 1395, 1562, 1908, 3228, 4070
AspLEI GCGC 5 cut(s) 168, 177, 938, 1036, 2708
AspS9I GGNCC 9 cut(s) 316, 557, 681, 773, 1022, 1223, 1774, 2103, 3266
AsuC2I CCSGG 2 cut(s) 2673, 3163
AsuHPI GGTGA 8 cut(s) 29, 280, 1457, 1873, 3154, 3260, 3403, 4220
AvaI CYCGRG 1 cut(s) 2064
AvaII GGWCC 7 cut(s) 316, 557, 681, 773, 1223, 1774, 3266
AxyI CCTNAGG 1 cut(s) 2910
BalI TGGCCA 1 cut(s) 2124
BamHI GGATCC 1 cut(s) 1818
BanI GGYRCC 1 cut(s) 1156
BanII GRGCYC 2 cut(s) 3665, 3858
BarI GAAGNNNNNNTAC 2 cut(s) 3938, 3970
BauI CACGAG 1 cut(s) 2220
BbsI GAAGAC 1 cut(s) 90
Bbv12I GWGCWC 4 cut(s) 43, 1257, 3858, 4302
BceAI ACGGC 3 cut(s) 2570, 3574, 3992
BcgI CGANNNNNNTGC 2 cut(s) 1418, 1452
BciT130I CCWGG 3 cut(s) 2600, 3003, 3115
BciVI GTATCC 1 cut(s) 2799
BclI TGATCA 3 cut(s) 1863, 3294, 3459
BcnI CCSGG 2 cut(s) 2673, 3163
BcoDI GTCTC 7 cut(s) 878, 2067, 2223, 3020, 3598, 4045, 4176
BfaI CTAG 3 cut(s) 534, 845, 3365
BfmI CTRYAG 5 cut(s) 276, 2133, 3171, 3375, 4035
BfoI RGCGCY 2 cut(s) 1037, 2709
BfrI CTTAAG 1 cut(s) 1823
BfuAI ACCTGC 1 cut(s) 3476
BfuI GTATCC 1 cut(s) 2799
BglII AGATCT 2 cut(s) 616, 3685
BlpI GCTNAGC 1 cut(s) 3470
BmcAI AGTACT 5 cut(s) 1337, 2037, 2352, 3302, 3518
Bme1390I CCNGG 5 cut(s) 2600, 2673, 3003, 3115, 3163
Bme18I GGWCC 7 cut(s) 316, 557, 681, 773, 1223, 1774, 3266
BmeT110I CYCGRG 1 cut(s) 2064
BmgT120I GGNCC 9 cut(s) 316, 557, 681, 773, 1022, 1223, 1774, 2103, 3266
BmiI GGNNCC 7 cut(s) 559, 1158, 1225, 1775, 1820, 2168, 3706
BmrFI CCNGG 5 cut(s) 2600, 2673, 3003, 3115, 3163
BmrI ACTGGG 2 cut(s) 1348, 4133
BmuI ACTGGG 2 cut(s) 1348, 4133
BpiI GAAGAC 1 cut(s) 90
BplI GAGNNNNNCTC 6 cut(s) 25, 57, 668, 700, 3083, 3115
BpmI CTGGAG 3 cut(s) 2415, 3024, 3465
Bpu1102I GCTNAGC 1 cut(s) 3470
BpuEI CTTGAG 7 cut(s) 296, 896, 947, 1262, 2328, 2425, 2598
BpuMI CCSGG 2 cut(s) 2673, 3163
Bsa29I ATCGAT 1 cut(s) 3315
BsaBI GATNNNNATC 3 cut(s) 218, 840, 1832
BsaI GGTCTC 2 cut(s) 878, 3020
BsaJI CCNNGG 9 cut(s) 78, 684, 1219, 1377, 1458, 2025, 2367, 2598, 3114
BsaWI WCCGGW 4 cut(s) 318, 1058, 2657, 2898
BsaXI ACNNNNNCTCC 6 cut(s) 2388, 2418, 2625, 2655, 3882, 3912
Bsc4I CCNNNNNNNGG 7 cut(s) 569, 1972, 2979, 3101, 3485, 3544, 3555
Bse118I RCCGGY 5 cut(s) 1024, 1058, 1153, 2657, 3760
Bse1I ACTGG 7 cut(s) 747, 1343, 2255, 3538, 3581, 3926, 4139
Bse21I CCTNAGG 1 cut(s) 2910
Bse3DI GCAATG 4 cut(s) 2499, 3705, 3821, 4026
Bse8I GATNNNNATC 3 cut(s) 218, 840, 1832
BseBI CCWGG 3 cut(s) 2600, 3003, 3115
BseCI ATCGAT 1 cut(s) 3315
BseDI CCNNGG 9 cut(s) 78, 684, 1219, 1377, 1458, 2025, 2367, 2598, 3114
BseJI GATNNNNATC 3 cut(s) 218, 840, 1832
BseLI CCNNNNNNNGG 7 cut(s) 569, 1972, 2979, 3101, 3485, 3544, 3555
BseMI GCAATG 4 cut(s) 2499, 3705, 3821, 4026
BseNI ACTGG 7 cut(s) 747, 1343, 2255, 3538, 3581, 3926, 4139
BseRI GAGGAG 4 cut(s) 2844, 3258, 3468, 4033
BsgI GTGCAG 3 cut(s) 3163, 3618, 4287
Bsh1236I CGCG 3 cut(s) 177, 330, 938
BshFI GGCC 9 cut(s) 1024, 2105, 2124, 2312, 2366, 2671, 3105, 3455, 3548
BshNI GGYRCC 1 cut(s) 1156
BshTI ACCGGT 2 cut(s) 1058, 2657
BshVI ATCGAT 1 cut(s) 3315
BsiHKAI GWGCWC 4 cut(s) 43, 1257, 3858, 4302
BsiHKCI CYCGRG 1 cut(s) 2064
BslFI GGGAC 4 cut(s) 936, 1209, 1787, 4268
BslI CCNNNNNNNGG 7 cut(s) 569, 1972, 2979, 3101, 3485, 3544, 3555
BsmAI GTCTC 7 cut(s) 878, 2067, 2223, 3020, 3598, 4045, 4176
BsmFI GGGAC 4 cut(s) 936, 1209, 1787, 4268
BsmI GAATGC 3 cut(s) 168, 180, 4107
BsnI GGCC 9 cut(s) 1024, 2105, 2124, 2312, 2366, 2671, 3105, 3455, 3548
Bso31I GGTCTC 2 cut(s) 878, 3020
BsoBI CYCGRG 1 cut(s) 2064
Bsp1286I GDGCHC 5 cut(s) 43, 1257, 3665, 3858, 4302
Bsp1720I GCTNAGC 1 cut(s) 3470
Bsp19I CCATGG 2 cut(s) 78, 1458
BspACI CCGC 8 cut(s) 177, 938, 981, 1343, 1470, 3587, 3747, 3803
BspANI GGCC 9 cut(s) 1024, 2105, 2124, 2312, 2366, 2671, 3105, 3455, 3548
BspDI ATCGAT 1 cut(s) 3315
BspFNI CGCG 3 cut(s) 177, 330, 938
BspHI TCATGA 2 cut(s) 1743, 1866
BspLI GGNNCC 7 cut(s) 559, 1158, 1225, 1775, 1820, 2168, 3706
BspMAI CTGCAG 2 cut(s) 280, 3379
BspMI ACCTGC 1 cut(s) 3476
BspPI GGATC 8 cut(s) 496, 830, 1813, 1822, 1826, 1886, 2637, 3663
BspQI GCTCTTC 1 cut(s) 1347
BspT107I GGYRCC 1 cut(s) 1156
BspTI CTTAAG 1 cut(s) 1823
BspTNI GGTCTC 2 cut(s) 878, 3020
BsrDI GCAATG 4 cut(s) 2499, 3705, 3821, 4026
BsrFI RCCGGY 5 cut(s) 1024, 1058, 1153, 2657, 3760
BsrI ACTGG 7 cut(s) 747, 1343, 2255, 3538, 3581, 3926, 4139
BssAI RCCGGY 5 cut(s) 1024, 1058, 1153, 2657, 3760
BssECI CCNNGG 9 cut(s) 78, 684, 1219, 1377, 1458, 2025, 2367, 2598, 3114
BssSI CACGAG 1 cut(s) 2220
BssT1I CCWWGG 6 cut(s) 78, 1219, 1377, 1458, 2025, 2367
Bst2BI CACGAG 1 cut(s) 2220
Bst2UI CCWGG 3 cut(s) 2600, 3003, 3115
Bst6I CTCTTC 9 cut(s) 202, 657, 1149, 1347, 1547, 1967, 2437, 3731, 4076
BstAFI CTTAAG 1 cut(s) 1823
BstC8I GCNNGC 5 cut(s) 1026, 1437, 1525, 1731, 2815
BstDSI CCRYGG 2 cut(s) 78, 1458
BstEII GGTNACC 1 cut(s) 1463
BstENI CCTNNNNNAGG 2 cut(s) 2977, 3483
BstFNI CGCG 3 cut(s) 177, 330, 938
BstH2I RGCGCY 2 cut(s) 1037, 2709
BstHHI GCGC 5 cut(s) 168, 177, 938, 1036, 2708
BstMAI GTCTC 7 cut(s) 878, 2067, 2223, 3020, 3598, 4045, 4176
BstNI CCWGG 3 cut(s) 2600, 3003, 3115
BstNSI RCATGY 2 cut(s) 2596, 4286
BstPI GGTNACC 1 cut(s) 1463
BstSCI CCNGG 5 cut(s) 2598, 2671, 3001, 3113, 3161
BstSFI CTRYAG 5 cut(s) 276, 2133, 3171, 3375, 4035
BstUI CGCG 3 cut(s) 177, 330, 938
BstV2I GAAGAC 1 cut(s) 90
BstX2I RGATCY 6 cut(s) 616, 835, 1818, 1827, 3655, 3685
BstXI CCANNNNNNTGG 1 cut(s) 4113
BstYI RGATCY 6 cut(s) 616, 835, 1818, 1827, 3655, 3685
Bsu15I ATCGAT 1 cut(s) 3315
Bsu36I CCTNAGG 1 cut(s) 2910
BsuI GTATCC 1 cut(s) 2799
BsuRI GGCC 9 cut(s) 1024, 2105, 2124, 2312, 2366, 2671, 3105, 3455, 3548
BsuTUI ATCGAT 1 cut(s) 3315
BtgI CCRYGG 2 cut(s) 78, 1458
BtgZI GCGATG 1 cut(s) 1443
BtsI GCAGTG 4 cut(s) 545, 869, 1701, 3606
BveI ACCTGC 1 cut(s) 3476
Cac8I GCNNGC 5 cut(s) 1026, 1437, 1525, 1731, 2815
CaiI CAGNNNCTG 1 cut(s) 839
CciI TCATGA 2 cut(s) 1743, 1866
CfoI GCGC 5 cut(s) 168, 177, 938, 1036, 2708
Cfr10I RCCGGY 5 cut(s) 1024, 1058, 1153, 2657, 3760
Cfr13I GGNCC 9 cut(s) 316, 557, 681, 773, 1022, 1223, 1774, 2103, 3266
ClaI ATCGAT 1 cut(s) 3315
CseI GACGC 1 cut(s) 1627
CspAI ACCGGT 2 cut(s) 1058, 2657
DraI TTTAAA 1 cut(s) 375
DraIII CACNNNGTG 1 cut(s) 4228
DrdI GACNNNNNNGTC 1 cut(s) 3926
DseDI GACNNNNNNGTC 1 cut(s) 3926
EaeI YGGCCR 1 cut(s) 2122
Eam1104I CTCTTC 9 cut(s) 202, 657, 1149, 1347, 1547, 1967, 2437, 3731, 4076
EarI CTCTTC 9 cut(s) 202, 657, 1149, 1347, 1547, 1967, 2437, 3731, 4076
EciI GGCGGA 2 cut(s) 996, 1358
Ecl136II GAGCTC 1 cut(s) 3856
Eco130I CCWWGG 6 cut(s) 78, 1219, 1377, 1458, 2025, 2367
Eco147I AGGCCT 1 cut(s) 2366
Eco24I GRGCYC 2 cut(s) 3665, 3858
Eco31I GGTCTC 2 cut(s) 878, 3020
Eco32I GATATC 4 cut(s) 220, 850, 1053, 3313
Eco47I GGWCC 7 cut(s) 316, 557, 681, 773, 1223, 1774, 3266
Eco47III AGCGCT 1 cut(s) 1035
Eco53kI GAGCTC 1 cut(s) 3856
Eco57I CTGAAG 4 cut(s) 1410, 2526, 2666, 3517
Eco81I CCTNAGG 1 cut(s) 2910
Eco88I CYCGRG 1 cut(s) 2064
Eco91I GGTNACC 1 cut(s) 1463
EcoICRI GAGCTC 1 cut(s) 3856
EcoNI CCTNNNNNAGG 2 cut(s) 2977, 3483
EcoO109I RGGNCCY 4 cut(s) 557, 681, 1223, 1774
EcoO65I GGTNACC 1 cut(s) 1463
EcoRI GAATTC 2 cut(s) 1538, 1558
EcoRII CCWGG 3 cut(s) 2598, 3001, 3113
EcoRV GATATC 4 cut(s) 220, 850, 1053, 3313
EcoT14I CCWWGG 6 cut(s) 78, 1219, 1377, 1458, 2025, 2367
EcoT38I GRGCYC 2 cut(s) 3665, 3858
ErhI CCWWGG 6 cut(s) 78, 1219, 1377, 1458, 2025, 2367
FaqI GGGAC 4 cut(s) 936, 1209, 1787, 4268
FauI CCCGC 2 cut(s) 1477, 3594
FauNDI CATATG 1 cut(s) 3829
FbaI TGATCA 3 cut(s) 1863, 3294, 3459
FblI GTMKAC 1 cut(s) 1569
FriOI GRGCYC 2 cut(s) 3665, 3858
FspBI CTAG 3 cut(s) 534, 845, 3365
FspI TGCGCA 1 cut(s) 167
GlaI GCGC 5 cut(s) 167, 176, 937, 1035, 2707
GsuI CTGGAG 3 cut(s) 2415, 3024, 3465
HaeII RGCGCY 2 cut(s) 1037, 2709
HaeIII GGCC 9 cut(s) 1024, 2105, 2124, 2312, 2366, 2671, 3105, 3455, 3548
HgaI GACGC 1 cut(s) 1627
HhaI GCGC 5 cut(s) 168, 177, 938, 1036, 2708
Hin6I GCGC 5 cut(s) 166, 175, 936, 1034, 2706
HinP1I GCGC 5 cut(s) 166, 175, 936, 1034, 2706
HincII GTYRAC 3 cut(s) 1657, 2590, 3406
HindII GTYRAC 3 cut(s) 1657, 2590, 3406
HindIII AAGCTT 2 cut(s) 390, 1521
HpaI GTTAAC 1 cut(s) 3406
HphI GGTGA 8 cut(s) 29, 280, 1457, 1873, 3154, 3260, 3403, 4220
Hpy166II GTNNAC 9 cut(s) 469, 1216, 1570, 1657, 2590, 2884, 3157, 3406, 3615
Hpy8I GTNNAC 9 cut(s) 469, 1216, 1570, 1657, 2590, 2884, 3157, 3406, 3615
HpyAV CCTTC 8 cut(s) 191, 853, 1501, 1787, 2641, 2759, 4060, 4330
HpyCH4IV ACGT 4 cut(s) 471, 778, 2073, 4151
HpySE526I ACGT 4 cut(s) 471, 778, 2073, 4151
HspAI GCGC 5 cut(s) 166, 175, 936, 1034, 2706
KroI GCCGGC 1 cut(s) 1024
KroNI GCCGGC 1 cut(s) 1026
Ksp22I TGATCA 3 cut(s) 1863, 3294, 3459
KspAI GTTAAC 1 cut(s) 3406
LguI GCTCTTC 1 cut(s) 1347
LmnI GCTCC 7 cut(s) 1330, 1976, 2166, 2264, 2956, 3466, 3861
MaeI CTAG 3 cut(s) 534, 845, 3365
MaeII ACGT 4 cut(s) 471, 778, 2073, 4151
MfeI CAATTG 2 cut(s) 351, 3126
MflI RGATCY 6 cut(s) 616, 835, 1818, 1827, 3655, 3685
MhlI GDGCHC 5 cut(s) 43, 1257, 3665, 3858, 4302
MlsI TGGCCA 1 cut(s) 2124
MluNI TGGCCA 1 cut(s) 2124
MlyI GAGTC 8 cut(s) 990, 1579, 1953, 2432, 2685, 2882, 3600, 4006
MmeI TCCRAC 6 cut(s) 237, 313, 371, 751, 2144, 2974
Mox20I TGGCCA 1 cut(s) 2124
MroNI GCCGGC 1 cut(s) 1024
MroXI GAANNNNTTC 5 cut(s) 1395, 1562, 1908, 3228, 4070
MscI TGGCCA 1 cut(s) 2124
MslI CAYNNNNRTG 5 cut(s) 375, 947, 1598, 1706, 4295
Msp20I TGGCCA 1 cut(s) 2124
MspA1I CMGCKG 2 cut(s) 3122, 4277
MspCI CTTAAG 1 cut(s) 1823
MspR9I CCNGG 5 cut(s) 2600, 2673, 3003, 3115, 3163
MunI CAATTG 2 cut(s) 351, 3126
Mva1269I GAATGC 3 cut(s) 168, 180, 4107
MvaI CCWGG 3 cut(s) 2600, 3003, 3115
MvnI CGCG 3 cut(s) 177, 330, 938
NaeI GCCGGC 1 cut(s) 1026
NciI CCSGG 2 cut(s) 2673, 3163
NcoI CCATGG 2 cut(s) 78, 1458
NdeI CATATG 1 cut(s) 3829
NgoMIV GCCGGC 1 cut(s) 1024
NlaIV GGNNCC 7 cut(s) 559, 1158, 1225, 1775, 1820, 2168, 3706
NmeAIII GCCGAG 2 cut(s) 665, 3797
NmuCI GTSAC 4 cut(s) 17, 1463, 2653, 4009
NsbI TGCGCA 1 cut(s) 167
NspI RCATGY 2 cut(s) 2596, 4286
PaeR7I CTCGAG 1 cut(s) 2064
PagI TCATGA 2 cut(s) 1743, 1866
PceI AGGCCT 1 cut(s) 2366
PciSI GCTCTTC 1 cut(s) 1347
PctI GAATGC 3 cut(s) 168, 180, 4107
PdiI GCCGGC 1 cut(s) 1026
PdmI GAANNNNTTC 5 cut(s) 1395, 1562, 1908, 3228, 4070
PfeI GAWTC 9 cut(s) 226, 1106, 1263, 2192, 2202, 2399, 2918, 4066, 4080
PflMI CCANNNNNTGG 3 cut(s) 569, 3101, 3544
PinAI ACCGGT 2 cut(s) 1058, 2657
PleI GAGTC 8 cut(s) 990, 1579, 1953, 2432, 2684, 2881, 3600, 4006
PpsI GAGTC 8 cut(s) 990, 1579, 1953, 2432, 2684, 2881, 3600, 4006
PpuMI RGGWCCY 4 cut(s) 557, 681, 1223, 1774
PshBI ATTAAT 1 cut(s) 324
Psp124BI GAGCTC 1 cut(s) 3858
Psp5II RGGWCCY 4 cut(s) 557, 681, 1223, 1774
Psp6I CCWGG 3 cut(s) 2598, 3001, 3113
PspEI GGTNACC 1 cut(s) 1463
PspGI CCWGG 3 cut(s) 2598, 3001, 3113
PspN4I GGNNCC 7 cut(s) 559, 1158, 1225, 1775, 1820, 2168, 3706
PspPI GGNCC 9 cut(s) 316, 557, 681, 773, 1022, 1223, 1774, 2103, 3266
PspPPI RGGWCCY 4 cut(s) 557, 681, 1223, 1774
PstI CTGCAG 2 cut(s) 280, 3379
PstNI CAGNNNCTG 1 cut(s) 839
PsuI RGATCY 6 cut(s) 616, 835, 1818, 1827, 3655, 3685
PvuII CAGCTG 2 cut(s) 3122, 4277
RseI CAYNNNNRTG 5 cut(s) 375, 947, 1598, 1706, 4295
SacI GAGCTC 1 cut(s) 3858
SapI GCTCTTC 1 cut(s) 1347
Sau96I GGNCC 9 cut(s) 316, 557, 681, 773, 1022, 1223, 1774, 2103, 3266
ScaI AGTACT 5 cut(s) 1337, 2037, 2352, 3302, 3518
SchI GAGTC 8 cut(s) 990, 1579, 1953, 2432, 2685, 2882, 3600, 4006
ScrFI CCNGG 5 cut(s) 2600, 2673, 3003, 3115, 3163
SduI GDGCHC 5 cut(s) 43, 1257, 3665, 3858, 4302
SfcI CTRYAG 5 cut(s) 276, 2133, 3171, 3375, 4035
Sfr274I CTCGAG 1 cut(s) 2064
SinI GGWCC 7 cut(s) 316, 557, 681, 773, 1223, 1774, 3266
SlaI CTCGAG 1 cut(s) 2064
SmiI ATTTAAAT 1 cut(s) 375
SmiMI CAYNNNNRTG 5 cut(s) 375, 947, 1598, 1706, 4295
SmlI CTYRAG 9 cut(s) 311, 911, 926, 1241, 1823, 2064, 2343, 2440, 2613
SmoI CTYRAG 9 cut(s) 311, 911, 926, 1241, 1823, 2064, 2343, 2440, 2613
SseBI AGGCCT 1 cut(s) 2366
SsiI CCGC 8 cut(s) 177, 938, 981, 1343, 1470, 3587, 3747, 3803
SspI AATATT 6 cut(s) 61, 423, 442, 3081, 3258, 3648
SspMI CTAG 3 cut(s) 534, 845, 3365
SstI GAGCTC 1 cut(s) 3858
StuI AGGCCT 1 cut(s) 2366
StyD4I CCNGG 5 cut(s) 2598, 2671, 3001, 3113, 3161
StyI CCWWGG 6 cut(s) 78, 1219, 1377, 1458, 2025, 2367
SwaI ATTTAAAT 1 cut(s) 375
TaiI ACGT 4 cut(s) 474, 781, 2076, 4154
TaqI TCGA 9 cut(s) 1417, 2065, 2455, 2632, 2763, 2975, 3219, 3315, 4200
TaqII GACCGA 1 cut(s) 3254
TatI WGTACW 9 cut(s) 1335, 2035, 2350, 3300, 3501, 3516, 3754, 3813, 3960
TauI GCSGC 3 cut(s) 180, 3749, 3806
TfiI GAWTC 9 cut(s) 226, 1106, 1263, 2192, 2202, 2399, 2918, 4066, 4080
TseFI GTSAC 4 cut(s) 17, 1463, 2653, 4009
Tsp45I GTSAC 4 cut(s) 17, 1463, 2653, 4009
TspGWI ACGGA 5 cut(s) 483, 663, 2392, 3903, 4173
Van91I CCANNNNNTGG 3 cut(s) 569, 3101, 3544
Vha464I CTTAAG 1 cut(s) 1823
VpaK11BI GGWCC 7 cut(s) 316, 557, 681, 773, 1223, 1774, 3266
VspI ATTAAT 1 cut(s) 324
XagI CCTNNNNNAGG 2 cut(s) 2977, 3483
XapI RAATTY 6 cut(s) 460, 623, 1382, 1538, 1558, 1897
XbaI TCTAGA 1 cut(s) 844
XceI RCATGY 2 cut(s) 2596, 4286
XcmI CCANNNNNNNNNTGG 4 cut(s) 586, 1121, 2365, 3082
XhoI CTCGAG 1 cut(s) 2064
XmiI GTMKAC 1 cut(s) 1569
XmnI GAANNNNTTC 5 cut(s) 1395, 1562, 1908, 3228, 4070
XspI CTAG 3 cut(s) 534, 845, 3365
ZrmI AGTACT 5 cut(s) 1337, 2037, 2352, 3302, 3518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.