Rorug06G0283400
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000493
Physical Location & Seq
Reverse (-)
43164187 .. 43171343
7157 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0283400.1

Sequence Viewer

Length: 3609 bp
ATGCAGCTAAGTAGGTCTGAGAACTTGCTTGGTACCATGACCGGTGAGGTGTCATGCTCATCAGGTCAATGGATCCAGCAAGAGTCGACCCCAGTTGTCCAGAATATGGGAAAGAATGTGCGCAGAAATGTCTCTGTGCAGACGGGGGAGGAGTTTTCCTTGGAGTTTCTTCAGGATTGTGGTGCGGTGAGAAGAGTTCCTTCTGCACCTGATACAGTTCTGAATTGTGAAAAGGGGGCTGGATTGAATTATAATCAGAATTCTACAGTGGCATATCAGGATCTTAGTGGTATTTTGGGGTTGAAGAGAATGGAGTCTGAGTGTGCATCTGACACATCTGGGTTTGTTGCGGCAAATGGGTCATGTAAAGAGATTGAAAATGAGGCTCATGTTGATAAGCTAAGCATACACAGCGTGAAGGAGGTTGATGGTGGAGAAGGATCAAGGAAGGCTGTTGGAGAACTAAATTGTGATCGAGCTGGTTTTCGGCCAACTGCTCCACCTGTGAACGTGTCTGAGTCTCCTCATTCTAACAACTTTAATCGGTCAGGAGTGTTGCATGGTTCTCAGTCTGGGAAGATGAAATTTCTTTGCAGTTTTGGTGGAAAGATTTTACCCAGGCCTAGTGATGGGAAACTCAGATATGTTGGGGGAGAGACGCGTTTTATTTCTATTAGGAATGGTCTTTCATGGGAAGAGCTTGTGAAGAAAACTTCCGGTTTTTGCAGCCAGCCTCATGTGATAAAATACCAGCTTCCAAATGAGGATCTTGATGCTCTTATATCTGTGTCATCTGATGAAGATCTTCAAAATATGATAGAGGAGTATCATGGGCTTGAAAGGCATGAGAGTTCTCAGAGGCCACGGGTCTTTTTGATTCCTCTGGGTGAGTCTGAAAATTGTTCCATTGAGGTTGACACCATACATTCGAGTAACCCTGATTACCAATATGTTGCTGCTGTAAATGATTTGGTAGATCCAAGTTCTAGGAAAACTAGTGGAGGACAGAATTTGAGTAGTGAAGCAAGTCAACAGGGAACAAAGACTTTGGTATTCCATAAGGAGATTCAGAGTAATTTTAAAGCCTTGCATCATAGTCAAATTTTGAGTGAGTCCCAGAATATGACTGGGTCTTCAATTCAATCACCTCTTTCTCCTATTCCACATCAACAAGCTGATGATAGGCAATGGGGCCAATCTCATGGAGGGCATTGTCACAACCACAATCACAGCAAAACTGTGGTGTCTACTTCAGCTGTTGGCCAAAACTTTGGTGACTTTGATGGGTTCTCCCGCGAGATGCCAGTGCATAAGGAAAGTAGATTTCTTTCCGACAAGCCTGTCTCACAGCCAGAAGATCTAATAGCTCAGTTGTCAGGTTCCATTGGTTGTCATCATGGCATGCCACATGCACTTTCTGATTCAAAACTACAAGAGAATGGAGGGAGGTCTGCATACTGTTCTCAAGAAGGAATGAACTTATCACACTCCTTAAACTTTGCAAACGCTCAATTGTTATCATTCCAGAACTCAGGAATTGCACAAGAAAAGCCAACCCAACTGCATGAAAACAGTGACCCTTTCAATCCTTGGGTACAAAACAAGTTAGAGGATATTGAGTCGGTAGGACTGCAGAGAAGACTGGATTTGCCAAATTCTTCTCCGTGTTTAGAATCATTGGGCAGGAATGAACATACTCAGAACGGTTCTGGTGACGTTCATGACAAATTCCGAACAGCTATAAAGAAAGATTCTATGACTTTGGAACTCCCAAATAAGTTGAATGAAAAGGATATTTTCTTGCATCATGATGACACCCTTTATGGGGAAATATCACCTGTCACTGGGGTGGAATGTCCGAAGAGATTGCCTAATATAAACCCCAATATAACATCTACTTTTGCCTCTGGGGTTGTGGTTCCTGCAGCCAGTGATTTCATACCTTTGGTAGATAATACAGTGCATGACCCCAAGAACTTCCAACTTGGCAAAACCCCTGCCAGTCTTCTTGTCACGAGTGAAGCAACTGCCAATGACCAGGATTGTGCTTTGACAGGGATGGTAAATGTTGAACAAGGACATGCTGTTTCAGCGGCTAAGAATCCTGAAGTTTCAGGCCGATTTCCAAGCACTAGAGAACACTCCTGTGATGGACGTTCTTTGGGTGATCTAATCTCAGGGTTGTCCAATGGACCAATTTCCCATGAACCTGTACATCCACAATTTGTTGCAAGTCAACATGATATAGGTTTCCAAGAACCCCTGCTCACAAGCTCTGCAAATTTTTACCCATCAACAGTTCTTGATGATCTTGAGTTCCAAAAGAGTGATCATATGGTGTTGCAGAAGCCAATCCAAAATGCTGCTTTTAAGAGAGAAGTATCCCTCCTTGATGATGATTTTGTGTGTTATCCTGGTACCAGTGTTGAGAATTTGAGCTTGAATGGTGAAAATGTCTCTTTGGCACAAACACAAGCCCAAACAATGAGTAATCATAAGGGACAGTTGGATACAGTAATAACTGCGGAAGATGTAAACAGTGGTGTTACTCCTGGCATTCAGTCTTTATTCGTGGTGTCCCCATATGTTGTTGACGAATCCATTGGTGATCTCATATCCCCAACTGCAACAGAGGTGGAGAGCATCATTCAAGAGTCTGAGTTTGAGGATGATAAGGGTGATATGGGAGACAAGAATGATTCATTTAGTGATGCAATGATAGTTGAAATGGAAGCCACCCTCTATGGTTTGCAGATCATTAGAAATGCTGATCTTGAAGAACTACGGGAATTGGGATCTGGTACATATGGAACTGTTTATCACGGAAAATGGCGGGGATCGGATGTTGCTATTAAGAGAATTAAAAAGGGCTGCTTTGCAGGGAGATCATCAGGGAAAGATCAATTGACAAAAGACTTTTGGAGAGAGGCACAGATCCTTTCAAATCTTCATCATCCAAATGTGGTTGCATTTTATGGGATAGTACCTGATGGAGCAGGAGGAACATTAGCAACTGTAGCTGAATATATGGTGAATGGATCCCTTAGGCATGCCCTACTGAAGAAGAATAGATCACTTGACCGGCGTAAAAAGCTTATAATTGCTATGGATGCAGCTTTTGGCATGGAATACTTGCATTCAAAGAATATTGTCCATTTTGATTTGAAGTGTGATAATTTGCTAGTCAACTTGAGGGATCCTCAACGACCTATATGTAAGGTTGGAGATTTTGGATTATCAAGAATCAAACGCAATACTTTGGTATCTGGTGGTGTGCGAGGAACACTTCCATGGATGGCACCAGAATTGTTGAACGGTAGCAGCAGCCGGGTCTCTGAGAAGGTTGATGTTTTCTCATTTGGAATTTCAATGTGGGAGATCTTGACTGGAGAGGAACCGTATGCAAATATGCATTGTGGTGCCATCATTGGGGGAATTGTAAAGAACACTCTTCGACCACCTATTCCAGAGCGATGTGATTCTGAGTGGAGGACGCTAATGGAAGAGTGCTGGTCACCTGATCCAGAAAGTCGGCCGTCATTTACTGAGATAACTAACAAGTTGCGAGCTATGTCCAACACACTTCAGGGAAAGGCTTCTGGTACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1202

Amino Acids

131.51

Weight (kDa)

5.44

Isoelectric Point (pI)

47.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 210 - 292 2.1e-19 PB1 domain
Pkinase PF00069 926 - 1186 1.6e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 927 - 1188 2e-63 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000493)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29820 FvH4_2g29820 FvH4_2g29822 FvH4_2g29824 FvH4_2g29824 FvH4_2g29824 FvH4_2g29840 FvH4_2g29840 FvH4_2g29840 FvH4_5g00550 FvH4_5g00550
malus_domestica MD02G1032700.v1.1 MD08G1140900.v1.1 MD08G1141400.v1.1 MD15G1117800.v1.1 MD15G1117900.v1.1
prunus_persica Prupe.1G471200_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471400_v2.0.a1 Prupe.7G242900_v2.0.a1
pyrus_communis pycom02g02690 pycom08g11890
rosa_chinensis RchiOBHm_Chr2g0111741 RchiOBHm_Chr2g0145991 RchiOBHm_Chr6g0298851 RchiOBHm_Chr6g0298871 RchiOBHm_Chr6g0298891 RchiOBHm_Chr6g0298901 RchiOBHm_Chr6g0298911 RchiOBHm_Chr7g0201801 RchiOBHm_Chr7g0211531 RchiOBHm_Chr7g0214601
rosa_laevigata RLG00000002931 RLG00000003000 RLG00000003647 RLG00000011476 RLG00000011479
rosa_multiflora Rmu_sc0001030.1_g000012 Rmu_sc0001548.1_g000041 Rmu_ssc0000397.1_g000033 Rmu_ssc0000397.1_g000036 Rmu_ssc0000397.1_g000037 Rmu_ssc0000397.1_g000039 Rmu_ssc0000397.1_g000048
rosa_roxburghii Rroxscaffold_1G00003830 Rroxscaffold_3G00247810 Rroxscaffold_3G00255060 Rroxscaffold_7G00169220 Rroxscaffold_7G00169230 Rroxscaffold_7G00169280
rosa_rugosa Rorug02G0223600 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283200 Rorug06G0283300 Rorug06G0283400 Rorug06G0283500 Rorug07G0066000
rosa_samantha Rh2AG511300 Rh2CG235100 Rh2CG496400 Rh2DG330900 Rh6BG403100 Rh6BG403300 Rh6BG403400 Rh6BG403500 Rh6CG408700 Rh6CG408900 Rh6CG409000 Rh6CG409200 Rh6DG395800 Rh6DG395900 Rh6DG396000 Rh6DG396200 Rh7AG193400 Rh7BG195100 Rh7BG436600 Rh7DG199300 Rh7DG265500
rosa_wichuraiana Rw2G016900 Rw6G034570 Rw6G034580 Rw6G034590 Rw6G034600 Rw7G016900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 252, 3098
AasI GACNNNNNNGTC 1 cut(s) 1340
Acc16I TGCGCA 1 cut(s) 122
Acc65I GGTACC 2 cut(s) 32, 2417
AccB1I GGYRCC 4 cut(s) 32, 2417, 3298, 3419
AccB7I CCANNNNNTGG 1 cut(s) 106
AccI GTMKAC 2 cut(s) 86, 1247
AccII CGCG 2 cut(s) 661, 1296
AciI CCGC 6 cut(s) 185, 350, 1294, 2093, 2525, 2833
AcoI YGGCCR 3 cut(s) 488, 1261, 3533
AcsI RAATTY 9 cut(s) 259, 584, 1009, 1101, 1654, 1727, 2281, 2431, 3363
AcuI CTGAAG 5 cut(s) 155, 1236, 2127, 3080, 3569
AdeI CACNNNGTG 1 cut(s) 415
AfaI GTAC 7 cut(s) 34, 1596, 2214, 2419, 2803, 2985, 3604
AfiI CCNNNNNNNGG 6 cut(s) 106, 629, 1824, 1825, 1844, 3429
AflIII ACRYGT 2 cut(s) 510, 659
AgeI ACCGGT 1 cut(s) 41
AhlI ACTAGT 1 cut(s) 995
AjnI CCWGG 4 cut(s) 617, 2037, 2413, 2551
AleI CACNNNNGTG 2 cut(s) 1847, 2145
Alw26I GTCTC 7 cut(s) 136, 525, 650, 1348, 2461, 2682, 3337
AoxI GGCC 7 cut(s) 488, 620, 860, 1192, 1261, 2116, 3533
ApeKI GCWGC 9 cut(s) 4, 726, 956, 1925, 2363, 2871, 3113, 3321, 3324
ApoI RAATTY 9 cut(s) 259, 584, 1009, 1101, 1654, 1727, 2281, 2431, 3363
AsiGI ACCGGT 1 cut(s) 41
Asp700I GAANNNNTTC 2 cut(s) 804, 3595
Asp718I GGTACC 2 cut(s) 32, 2417
AspLEI GCGC 1 cut(s) 123
AspS9I GGNCC 2 cut(s) 1192, 2192
AsuC2I CCSGG 1 cut(s) 3329
AvaII GGWCC 1 cut(s) 2192
AxyI CCTNAGG 1 cut(s) 3044
BaeI ACNNNNGTAYC 4 cut(s) 2409, 2442, 3246, 3279
BalI TGGCCA 1 cut(s) 1263
BamHI GGATCC 3 cut(s) 72, 3038, 3196
BanI GGYRCC 4 cut(s) 32, 2417, 3298, 3419
BauI CACGAG 1 cut(s) 2014
BbsI GAAGAC 3 cut(s) 1125, 1645, 1997
BbvI GCAGC 9 cut(s) 16, 738, 943, 1937, 2350, 2858, 3125, 3333, 3336
BccI CCATC 9 cut(s) 422, 623, 1277, 2053, 2144, 2299, 2984, 3289, 3431
BceAI ACGGC 1 cut(s) 3520
BcgI CGANNNNNNTGC 2 cut(s) 1849, 1883
BciT130I CCWGG 4 cut(s) 619, 2039, 2415, 2553
BciVI GTATCC 2 cut(s) 2392, 2503
BclI TGATCA 1 cut(s) 2329
BcnI CCSGG 1 cut(s) 3329
BcoDI GTCTC 7 cut(s) 136, 525, 650, 1348, 2461, 2682, 3337
BcuI ACTAGT 1 cut(s) 995
BfaI CTAG 5 cut(s) 624, 987, 996, 2133, 3182
BfmI CTRYAG 4 cut(s) 264, 1631, 1923, 3015
BfuI GTATCC 2 cut(s) 2392, 2503
BglII AGATCT 3 cut(s) 802, 1357, 3378
BlpI GCTNAGC 1 cut(s) 401
Bme1390I CCNGG 5 cut(s) 619, 2039, 2415, 2553, 3329
Bme18I GGWCC 1 cut(s) 2192
BmgT120I GGNCC 2 cut(s) 1192, 2192
BmrFI CCNGG 5 cut(s) 619, 2039, 2415, 2553, 3329
BmrI ACTGGG 3 cut(s) 86, 1137, 1854
BmsI GCATC 8 cut(s) 335, 763, 1099, 1290, 1813, 2652, 2701, 3100
BmuI ACTGGG 3 cut(s) 86, 1137, 1854
BpiI GAAGAC 3 cut(s) 1125, 1645, 1997
BplI GAGNNNNNCTC 2 cut(s) 3184, 3216
BpmI CTGGAG 1 cut(s) 3408
Bpu1102I GCTNAGC 1 cut(s) 401
BpuEI CTTGAG 3 cut(s) 1449, 2333, 3211
BpuMI CCSGG 1 cut(s) 3329
BsaAI YACGTR 1 cut(s) 3606
BsaBI GATNNNNATC 1 cut(s) 801
BsaI GGTCTC 1 cut(s) 3337
BsaJI CCNNGG 5 cut(s) 159, 617, 863, 1589, 3290
BsaWI WCCGGW 2 cut(s) 41, 716
BsaXI ACNNNNNCTCC 2 cut(s) 305, 335
Bsc4I CCNNNNNNNGG 6 cut(s) 106, 629, 1824, 1825, 1844, 3429
Bse118I RCCGGY 2 cut(s) 41, 3081
Bse1I ACTGG 9 cut(s) 92, 1132, 1304, 1647, 1849, 1929, 2001, 2421, 3391
Bse21I CCTNAGG 1 cut(s) 3044
Bse3DI GCAATG 2 cut(s) 1193, 2721
Bse8I GATNNNNATC 1 cut(s) 801
BseBI CCWGG 4 cut(s) 619, 2039, 2415, 2553
BseDI CCNNGG 5 cut(s) 159, 617, 863, 1589, 3290
BseGI GGATG 7 cut(s) 2064, 2215, 2674, 2848, 2953, 3115, 3300
BseJI GATNNNNATC 1 cut(s) 801
BseLI CCNNNNNNNGG 6 cut(s) 106, 629, 1824, 1825, 1844, 3429
BseMI GCAATG 2 cut(s) 1193, 2721
BseNI ACTGG 9 cut(s) 92, 1132, 1304, 1647, 1849, 1929, 2001, 2421, 3391
BseRI GAGGAG 3 cut(s) 164, 513, 836
BseX3I CGGCCG 1 cut(s) 3533
BseXI GCAGC 9 cut(s) 16, 738, 943, 1937, 2350, 2858, 3125, 3333, 3336
BsgI GTGCAG 2 cut(s) 158, 189
Bsh1236I CGCG 2 cut(s) 661, 1296
Bsh1285I CGRYCG 1 cut(s) 3536
BshFI GGCC 7 cut(s) 490, 622, 862, 1194, 1263, 2118, 3535
BshNI GGYRCC 4 cut(s) 32, 2417, 3298, 3419
BshTI ACCGGT 1 cut(s) 41
BsiEI CGRYCG 1 cut(s) 3536
BsiSI CCGG 4 cut(s) 42, 717, 3082, 3328
BslFI GGGAC 3 cut(s) 1099, 2514, 2563
BslI CCNNNNNNNGG 6 cut(s) 106, 629, 1824, 1825, 1844, 3429
BsmAI GTCTC 7 cut(s) 136, 525, 650, 1348, 2461, 2682, 3337
BsmBI CGTCTC 1 cut(s) 650
BsmFI GGGAC 3 cut(s) 1099, 2514, 2563
BsmI GAATGC 2 cut(s) 2556, 3136
BsnI GGCC 7 cut(s) 490, 622, 862, 1194, 1263, 2118, 3535
Bso31I GGTCTC 1 cut(s) 3337
Bsp1407I TGTACA 1 cut(s) 2212
Bsp1720I GCTNAGC 1 cut(s) 401
Bsp19I CCATGG 1 cut(s) 3290
BspACI CCGC 6 cut(s) 185, 350, 1294, 2093, 2525, 2833
BspANI GGCC 7 cut(s) 490, 622, 862, 1194, 1263, 2118, 3535
BspFNI CGCG 2 cut(s) 661, 1296
BspHI TCATGA 2 cut(s) 1720, 1807
BspMAI CTGCAG 2 cut(s) 1635, 1927
BspQI GCTCTTC 1 cut(s) 690
BspT107I GGYRCC 4 cut(s) 32, 2417, 3298, 3419
BspTNI GGTCTC 1 cut(s) 3337
BsrDI GCAATG 2 cut(s) 1193, 2721
BsrFI RCCGGY 2 cut(s) 41, 3081
BsrGI TGTACA 1 cut(s) 2212
BsrI ACTGG 9 cut(s) 92, 1132, 1304, 1647, 1849, 1929, 2001, 2421, 3391
BssAI RCCGGY 2 cut(s) 41, 3081
BssECI CCNNGG 5 cut(s) 159, 617, 863, 1589, 3290
BssSI CACGAG 1 cut(s) 2014
BssT1I CCWWGG 3 cut(s) 159, 1589, 3290
Bst2BI CACGAG 1 cut(s) 2014
Bst2UI CCWGG 4 cut(s) 619, 2039, 2415, 2553
Bst6I CTCTTC 6 cut(s) 187, 299, 690, 1856, 3456, 3498
BstAUI TGTACA 1 cut(s) 2212
BstBAI YACGTR 1 cut(s) 3606
BstC8I GCNNGC 4 cut(s) 731, 1403, 3051, 3567
BstDSI CCRYGG 2 cut(s) 863, 3290
BstEII GGTNACC 1 cut(s) 3513
BstF5I GGATG 7 cut(s) 2064, 2215, 2674, 2848, 2953, 3115, 3300
BstFNI CGCG 2 cut(s) 661, 1296
BstHHI GCGC 1 cut(s) 123
BstMAI GTCTC 7 cut(s) 136, 525, 650, 1348, 2461, 2682, 3337
BstMCI CGRYCG 1 cut(s) 3536
BstMWI GCNNNNNNNGC 6 cut(s) 411, 841, 2090, 3017, 3091, 3110
BstNI CCWGG 4 cut(s) 619, 2039, 2415, 2553
BstNSI RCATGY 4 cut(s) 1405, 1412, 2084, 3053
BstPI GGTNACC 1 cut(s) 3513
BstSCI CCNGG 5 cut(s) 617, 2037, 2413, 2551, 3327
BstSFI CTRYAG 4 cut(s) 264, 1631, 1923, 3015
BstSNI TACGTA 1 cut(s) 3606
BstUI CGCG 2 cut(s) 661, 1296
BstV1I GCAGC 9 cut(s) 16, 738, 943, 1937, 2350, 2858, 3125, 3333, 3336
BstV2I GAAGAC 3 cut(s) 1125, 1645, 1997
BstXI CCANNNNNNTGG 2 cut(s) 1202, 1271
BstZI CGGCCG 1 cut(s) 3533
Bsu36I CCTNAGG 1 cut(s) 3044
BsuI GTATCC 2 cut(s) 2392, 2503
BsuRI GGCC 7 cut(s) 490, 622, 862, 1194, 1263, 2118, 3535
BtgI CCRYGG 2 cut(s) 863, 3290
BtgZI GCGATG 1 cut(s) 3487
BtsCI GGATG 7 cut(s) 2064, 2215, 2674, 2848, 2953, 3115, 3300
BtsIMutI CAGTG 8 cut(s) 273, 1311, 1579, 1842, 1936, 1965, 2428, 2545
Cac8I GCNNGC 4 cut(s) 731, 1403, 3051, 3567
CciI TCATGA 2 cut(s) 1720, 1807
CfoI GCGC 1 cut(s) 123
Cfr10I RCCGGY 2 cut(s) 41, 3081
Cfr13I GGNCC 2 cut(s) 1192, 2192
CseI GACGC 2 cut(s) 667, 3502
Csp6I GTAC 7 cut(s) 33, 1595, 2213, 2418, 2802, 2984, 3603
CspAI ACCGGT 1 cut(s) 41
CspCI CAANNNNNGTGG 2 cut(s) 2616, 2651
CviQI GTAC 7 cut(s) 33, 1595, 2213, 2418, 2802, 2984, 3603
DraI TTTAAA 1 cut(s) 1081
DraIII CACNNNGTG 1 cut(s) 415
DrdI GACNNNNNNGTC 1 cut(s) 1340
DseDI GACNNNNNNGTC 1 cut(s) 1340
EaeI YGGCCR 3 cut(s) 488, 1261, 3533
EagI CGGCCG 1 cut(s) 3533
Eam1104I CTCTTC 6 cut(s) 187, 299, 690, 1856, 3456, 3498
EarI CTCTTC 6 cut(s) 187, 299, 690, 1856, 3456, 3498
EclXI CGGCCG 1 cut(s) 3533
Eco105I TACGTA 1 cut(s) 3606
Eco130I CCWWGG 3 cut(s) 159, 1589, 3290
Eco147I AGGCCT 1 cut(s) 622
Eco31I GGTCTC 1 cut(s) 3337
Eco47I GGWCC 1 cut(s) 2192
Eco52I CGGCCG 1 cut(s) 3533
Eco57I CTGAAG 5 cut(s) 155, 1236, 2127, 3080, 3569
Eco81I CCTNAGG 1 cut(s) 3044
Eco91I GGTNACC 1 cut(s) 3513
EcoO65I GGTNACC 1 cut(s) 3513
EcoRI GAATTC 1 cut(s) 259
EcoRII CCWGG 4 cut(s) 617, 2037, 2413, 2551
EcoT14I CCWWGG 3 cut(s) 159, 1589, 3290
EcoT22I ATGCAT 1 cut(s) 3414
ErhI CCWWGG 3 cut(s) 159, 1589, 3290
Esp3I CGTCTC 1 cut(s) 650
FalI AAGNNNNNCTT 4 cut(s) 184, 216, 2858, 2890
FaqI GGGAC 3 cut(s) 1099, 2514, 2563
FauI CCCGC 2 cut(s) 1301, 2826
FauNDI CATATG 3 cut(s) 2334, 2584, 2806
FbaI TGATCA 1 cut(s) 2329
FblI GTMKAC 2 cut(s) 86, 1247
FokI GGATG 7 cut(s) 2071, 2202, 2681, 2855, 2940, 3122, 3307
FspBI CTAG 5 cut(s) 624, 987, 996, 2133, 3182
FspI TGCGCA 1 cut(s) 122
GlaI GCGC 1 cut(s) 122
GsuI CTGGAG 1 cut(s) 3408
HaeIII GGCC 7 cut(s) 490, 622, 862, 1194, 1263, 2118, 3535
HapII CCGG 4 cut(s) 42, 717, 3082, 3328
HgaI GACGC 2 cut(s) 667, 3502
HhaI GCGC 1 cut(s) 123
Hin6I GCGC 1 cut(s) 121
HinP1I GCGC 1 cut(s) 121
HincII GTYRAC 6 cut(s) 87, 916, 1031, 2237, 2593, 3187
HindII GTYRAC 6 cut(s) 87, 916, 1031, 2237, 2593, 3187
HindIII AAGCTT 1 cut(s) 3092
HpaII CCGG 4 cut(s) 42, 717, 3082, 3328
Hpy166II GTNNAC 9 cut(s) 87, 508, 916, 1031, 1248, 2237, 2536, 2593, 3187
Hpy8I GTNNAC 9 cut(s) 87, 508, 916, 1031, 1248, 2237, 2536, 2593, 3187
HpyAV CCTTC 6 cut(s) 210, 412, 431, 442, 1463, 3334
HpyCH4IV ACGT 4 cut(s) 510, 1716, 2155, 3605
HpyF10VI GCNNNNNNNGC 6 cut(s) 411, 841, 2090, 3017, 3091, 3110
HpySE526I ACGT 4 cut(s) 510, 1716, 2155, 3605
HspAI GCGC 1 cut(s) 121
KpnI GGTACC 2 cut(s) 36, 2421
Ksp22I TGATCA 1 cut(s) 2329
LguI GCTCTTC 1 cut(s) 690
LmnI GCTCC 2 cut(s) 502, 2993
Lsp1109I GCAGC 9 cut(s) 16, 738, 943, 1937, 2350, 2858, 3125, 3333, 3336
LweI GCATC 8 cut(s) 335, 763, 1099, 1290, 1813, 2652, 2701, 3100
MaeI CTAG 5 cut(s) 624, 987, 996, 2133, 3182
MaeII ACGT 4 cut(s) 510, 1716, 2155, 3605
MaeIII GTNAC 9 cut(s) 932, 1214, 1274, 1574, 1712, 1840, 2011, 2545, 3513
MfeI CAATTG 2 cut(s) 1511, 2903
MlsI TGGCCA 1 cut(s) 1263
MluI ACGCGT 1 cut(s) 659
MluNI TGGCCA 1 cut(s) 1263
MlyI GAGTC 7 cut(s) 92, 323, 527, 899, 1121, 1628, 2663
MmeI TCCRAC 6 cut(s) 436, 1356, 2005, 2487, 3202, 3600
Mox20I TGGCCA 1 cut(s) 1263
Mph1103I ATGCAT 1 cut(s) 3414
MroXI GAANNNNTTC 2 cut(s) 804, 3595
MscI TGGCCA 1 cut(s) 1263
MseI TTAA 6 cut(s) 540, 1080, 1493, 2370, 2853, 2862
MslI CAYNNNNRTG 6 cut(s) 1809, 1847, 2145, 2958, 3289, 3417
Msp20I TGGCCA 1 cut(s) 1263
MspA1I CMGCKG 2 cut(s) 1256, 2093
MspI CCGG 4 cut(s) 42, 717, 3082, 3328
MspR9I CCNGG 5 cut(s) 619, 2039, 2415, 2553, 3329
MunI CAATTG 2 cut(s) 1511, 2903
Mva1269I GAATGC 2 cut(s) 2556, 3136
MvaI CCWGG 4 cut(s) 619, 2039, 2415, 2553
MvnI CGCG 2 cut(s) 661, 1296
MwoI GCNNNNNNNGC 6 cut(s) 411, 841, 2090, 3017, 3091, 3110
NciI CCSGG 1 cut(s) 3329
NcoI CCATGG 1 cut(s) 3290
NdeI CATATG 3 cut(s) 2334, 2584, 2806
NmuCI GTSAC 7 cut(s) 1214, 1274, 1574, 1712, 1840, 2011, 3513
NsbI TGCGCA 1 cut(s) 122
NsiI ATGCAT 1 cut(s) 3414
NspI RCATGY 4 cut(s) 1405, 1412, 2084, 3053
OliI CACNNNNGTG 2 cut(s) 1847, 2145
PaeI GCATGC 2 cut(s) 1405, 3053
PagI TCATGA 2 cut(s) 1720, 1807
PceI AGGCCT 1 cut(s) 622
PciSI GCTCTTC 1 cut(s) 690
PctI GAATGC 2 cut(s) 2556, 3136
PdmI GAANNNNTTC 2 cut(s) 804, 3595
PflFI GACNNNGTC 1 cut(s) 1129
PflMI CCANNNNNTGG 1 cut(s) 106
PinAI ACCGGT 1 cut(s) 41
PleI GAGTC 7 cut(s) 91, 322, 526, 898, 1120, 1627, 2662
PpsI GAGTC 7 cut(s) 91, 322, 526, 898, 1120, 1627, 2662
Ppu21I YACGTR 1 cut(s) 3606
PsiI TTATAA 2 cut(s) 252, 3098
Psp6I CCWGG 4 cut(s) 617, 2037, 2413, 2551
PspEI GGTNACC 1 cut(s) 3513
PspGI CCWGG 4 cut(s) 617, 2037, 2413, 2551
PspPI GGNCC 2 cut(s) 1192, 2192
PstI CTGCAG 2 cut(s) 1635, 1927
PsyI GACNNNGTC 1 cut(s) 1129
PvuII CAGCTG 1 cut(s) 1256
RsaI GTAC 7 cut(s) 34, 1596, 2214, 2419, 2803, 2985, 3604
RsaNI GTAC 7 cut(s) 33, 1595, 2213, 2418, 2802, 2984, 3603
RseI CAYNNNNRTG 6 cut(s) 1809, 1847, 2145, 2958, 3289, 3417
SalI GTCGAC 1 cut(s) 85
SapI GCTCTTC 1 cut(s) 690
SaqAI TTAA 6 cut(s) 540, 1080, 1493, 2370, 2853, 2862
Sau96I GGNCC 2 cut(s) 1192, 2192
SchI GAGTC 7 cut(s) 92, 323, 527, 899, 1121, 1628, 2663
ScrFI CCNGG 5 cut(s) 619, 2039, 2415, 2553, 3329
SfaNI GCATC 8 cut(s) 335, 763, 1099, 1290, 1813, 2652, 2701, 3100
SfcI CTRYAG 4 cut(s) 264, 1631, 1923, 3015
SinI GGWCC 1 cut(s) 2192
SmiMI CAYNNNNRTG 6 cut(s) 1809, 1847, 2145, 2958, 3289, 3417
SmlI CTYRAG 3 cut(s) 1464, 2312, 3190
SmoI CTYRAG 3 cut(s) 1464, 2312, 3190
SnaBI TACGTA 1 cut(s) 3606
SpeI ACTAGT 1 cut(s) 995
SphI GCATGC 2 cut(s) 1405, 3053
SseBI AGGCCT 1 cut(s) 622
SsiI CCGC 6 cut(s) 185, 350, 1294, 2093, 2525, 2833
SspI AATATT 1 cut(s) 3148
SspMI CTAG 5 cut(s) 624, 987, 996, 2133, 3182
StuI AGGCCT 1 cut(s) 622
StyD4I CCNGG 5 cut(s) 617, 2037, 2413, 2551, 3327
StyI CCWWGG 3 cut(s) 159, 1589, 3290
TaiI ACGT 4 cut(s) 513, 1719, 2158, 3608
TaqI TCGA 4 cut(s) 86, 475, 929, 3454
TaqII GACCGA 1 cut(s) 534
TatI WGTACW 1 cut(s) 2212
TauI GCSGC 2 cut(s) 353, 2096
Tru1I TTAA 6 cut(s) 540, 1080, 1493, 2370, 2853, 2862
Tru9I TTAA 6 cut(s) 540, 1080, 1493, 2370, 2853, 2862
TscAI CASTG 8 cut(s) 273, 1311, 1579, 1849, 1936, 1965, 2428, 2545
TseFI GTSAC 7 cut(s) 1214, 1274, 1574, 1712, 1840, 2011, 3513
TseI GCWGC 9 cut(s) 4, 726, 956, 1925, 2363, 2871, 3113, 3321, 3324
Tsp45I GTSAC 7 cut(s) 1214, 1274, 1574, 1712, 1840, 2011, 3513
TspGWI ACGGA 2 cut(s) 1653, 2838
TspRI CASTG 8 cut(s) 273, 1311, 1579, 1849, 1936, 1965, 2428, 2545
Tth111I GACNNNGTC 1 cut(s) 1129
Van91I CCANNNNNTGG 1 cut(s) 106
VpaK11BI GGWCC 1 cut(s) 2192
XapI RAATTY 9 cut(s) 259, 584, 1009, 1101, 1654, 1727, 2281, 2431, 3363
XceI RCATGY 4 cut(s) 1405, 1412, 2084, 3053
XcmI CCANNNNNNNNNTGG 1 cut(s) 1124
XmiI GTMKAC 2 cut(s) 86, 1247
XmnI GAANNNNTTC 2 cut(s) 804, 3595
XspI CTAG 5 cut(s) 624, 987, 996, 2133, 3182
Zsp2I ATGCAT 1 cut(s) 3414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.