MD08G1140900.v1.1
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000493
Physical Location & Seq
Reverse (-)
13895153 .. 13901352
6200 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1140900.v1.1.491

Sequence Viewer

Length: 3870 bp
ATGTTGCAGGGAATGCTACATGATCTTCGAATTTTTCCGAGTAGAAAGATACCTCTAAAAATCCTTCAAAACATTAGTGGAATTATCAAGCCTCAAAGAATGACTCTACTCTTAGGTCCCCCAAGCTCTGGAAAAACCACATTACTATTGGCGTTGGCCGGAAGACTTGGTAGAGATTTAAAATCTTCCGGGCGGGTTACATATAACGGACATGGGATGGAAGAATTTGTACCTCAGAGAACATCGGCTTATGTAAGTCAAAACGATCTCCATATAGGAGAGCTGACAGTGAAAGAGACATTTTCTTTCTCGGCTAGATGTCAAGGAGTTGGATCAAATTGCGACATGTTGGTGGAACTGTGTAGAAGAGAGAAGGAGGGAAATATCAAGCCTGATCCTGACATTGACATATACCTGAAGGCAGCGGCTCTAGAAGGGCAGGAGACCAATGTAGTTACTGATTACATTCTCAAGATTCTCGGACTTGAAGTCTGTGCTAATACTATGGTGGGAGATGATATGGTAAGAGGCATTTCTGGTGGAGAAAGAAAGCGAGTCACGATAGGGGAGATGCTTGTTGGACCGGTAAAAGCTCTCTTCATGGATGAGATTTCAACCGGCTTGGACAGTTCGACAACTTTCCAAATAGTAAATTCAATCAGGCAATCCATCCACATTCTAAGTGGAACTGCAGTTGTCTCCCTCCTCCAGCCAGCCCCTGAAACTTATAACCTCTTTGATGATATAATTCTTCTATCAGATGGACAAATCGTGTATCAGGGTCCACGTGAAAATGTGCTCGAGTTTTTTGAATCCATGGGCTTCATATGTCCGGAGAGAAAAGGAGTTGCTGACTTCTTGCAAGAGGTGACATCAAAGAAGGATCAAGAGCAGTACTGGGCATGTCGAGACAAGCCTTACAGATTTGTTACTGTCAATGAGTTTGTCGAAGCTTTCCAGTCATTTCCAATTGGTCAGAAACTCGGCGACGAGCTTGCTACTCCCTTCGACAAGTCCAGAAGTCATCGTGCTGCTTTATCATCTAAAAGGTATGGCGTTAACAATAAGGAGTTGTTCCGAGCATGTGTTTCAAGAGAGTTCTTGCTCATGAAGAGGAACTCATTTGCCTACATTTTCAAACTTACCCTGCTTATTATCTTAGCTTCTATAACATCATCGATATTTCTACGAATCAAGATGCATAAGGACTCAGTGGTGGATGGTGGAGTTTACATGGGAGCTTTGTTCTTCGCAGTCATTGTAGCGATGTTTAACGGAATATCAGAACTCAACATGGCAATTATGAAACTTCCTGTCTTTTACAAGCAGAGAGACCTTCTCTTCTTTCCTTCCTGGGCTTACTCACTTCCTGCATGGATCCTCAAGATCCCAATCAGTTTGGCCGAAAGTGCCATTTGGGTTGTCATTACTTATTATGTAATAGGATTTGATCCATATGTTGAAAGGTTCTTCAAGCAGTACATTGTACTCTTGTGCATAAACCAGATGGCGTCTGGGCTGTTCAGGTTGATAGCAGCCTTAGGAAGGGATGTAACAGTTGCAAGCACAACAGGATCCTTTGCACTGCTTATAATTATGGTTTTGGGCGGATTCGTCTTATCTCGAGATGCTGTTCCAAAATGGTGGCTGTGGGGTTACTGGGTCTCGCCGTTGATGTATGGACAAAATGCTATTACTGTGAATGAATTTCTTGGAAAGAGTTGGAGACAAGTTCCACCTAATTCTACAGAATCCTTGGGAGTTCTGATCCTTAAGTCTCATGGAATTTTTCCAGAAGCAAAATTGTTTTGGATTGGAGTAGCAGCATTGATTGGATATATTTTTCTATTCAATCTCCTCCTTACTTTGGCTCTGCATTATCTTGATCCACTCGGGAAGCCTCAGACAGTACTAACCAAAGAAAGCATGGCTGAGCGAAATTTTAGCAGAAATGGAGAGTTCATTGAGCTAATACCAAGAGGAAAGATCTCTTCTGAATCCGAAAGAGTGGGAAGAACTGATGAAGCTAATGGAAAAAGGAAGCGAGGGATGGTTCTTCCTTTTCATCCCCTTTCCCTCACTTTCGATGAGATCAGATATGCCATAGATATGCCACAAGAAATGAAAGCGGAGGGTGTTCAGGAGGATCAGCTTGAACTTCTGAAGGGTGTAAGTGGTGCTTTTCGGCCAGGAGTCCTAACAGCGTTAATGGGAGTCAGTGGTGCCGGCAAGACCACTCTAATGGATGTGTTGGCTGGAAGGAAAACTGGTGGACATATCAAGGGAAACATCACGATCTCTGGCTATCCTAAAAGGCAAGAAACATTTGCTCGCATATCTGGATATTGTGAGCAAACTGATATCCACTCTCCCCATGTGACAGTCTATGAATCTCTACTTTTTTCTGCCTGGCTCCGGCTACTACCTGAGGTTGATTCTGCAACAAGAGAGATGTTTATCGAGGAAGTCATGGAACTTGTGGAGCTGACTTCATTCAGGGGAGCTCTTGTGGGATTACCTGGTGTAACCGGTCTGTCAACTGAGCAGCGCAAAAGGCTAACGATCGCAGTTGAGCTCGTTGCAAATCCATCGATAATCTTCATGGACGAACCAACATCTGGCCTCGATGCTAGGGCAGCAGCGATTGTGATGAGAACAGTGAGGAACACAGTGGACACGGGAAGAACCGTGGTTTGTACCATCCACCAGCCAAGCATAGATATATTCGATGCTTTCGATGAGCTTCTTCTTCTGAAATTGGGAGGTGAGCAAATATATGCCGGTCCATTAGGCTCCCATTGCTCCATTCTGATCAATTATTTTGAGGGAATCAATGGAGTTCCAAAGATTAAAGATGGATATAACCCGGCAACCTGGATGCTGGAGGTTACTTCAGCCGCACAAGAGGCAGCTCTTGGGGTTAACTTCACAGATGCGTACAATAACTCGGAAATATACAAGAGAACCAAAGCAATGATTGAGGAATTAAACACACCTCCCTCGGGTTCTAAAGATCTATACTTCCCTACTCGCTACTCACAATCTTTTCCCACCCAATGTATGGCTTGCCTGTGGAAACAGCACTGGTCATATTGGCGAAACCCATCATATAGTGCAGTAAGGCTGTTGTTCACAACGTTCATCGCTTTATTATTCGGTTTGATATTCTGGAACCTTGGATCAAAAAGTAAGCAGCAAGATCTCTTGAATGCAATGGGTTCCATGTACGCTGCTGTTATCTTTATCGGGGTACAAAATGGTGCCTCAGTGCAGCCGGTTGTGGCTGTTGAGAGAACAGTCTTTTACAGAGAAAGAGCAGCTGGAATGTATTCCGCTTTGCCATACGCATTTGGGCAGATGATGATTGAGGTTCCCTACATCTTCATTCAGACCGTTATTTACGGAGCTATAGTATATGCCATGATGGGATTCGATTGGATAGCTGCCAAGTTCTTATGGTACCTTTTCTTCATGTACTTCACCTTGCTGTACTTCACATTCTACGGCATGATGACCATGGCTCTCACTCCGAACCACAACATTGCTGCAATAACCTCATCTACCTTCTACGCAATCTGGAACCTCTTCTCGGGATTCGTTGTTCCCCCAACGAGGATTCCGATATGGTGGAAATGGTACTATTGGGTCTGCCCTGTTGCCTGGACCTTGTATGGAATGGTTGCTTCGCAATTTGGAGACGTAAAGGAGAAACTGGAGTCAGGAGAATCGGTAGAGCATTTTTTGAGAAGTTACTTCGGATACAAGCATGAGTTTCTGGGCATCGTTGCAGTTGTGATTGTTGGGTTTTCATTGCTGTTTGGGTTCATCTTTGCCTATGCAATTAGAGCATTTAATTTCCAAAAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1290

Amino Acids

144.77

Weight (kDa)

8.26

Isoelectric Point (pI)

38.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 22 - 203 3e-15 ABC transporter
ABC2_membrane_7 PF19055 237 - 290 1.3e-06 ABC-2 type transporter
ABC2_membrane PF01061 358 - 570 2e-48 ABC-2 type transporter
PDR_assoc PF08370 575 - 637 7.3e-25 Plant PDR ABC transporter associated
ABC_tran PF00005 721 - 873 6.8e-20 ABC transporter
ABC2_membrane PF01061 1018 - 1231 1.1e-58 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000493)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29820 FvH4_2g29820 FvH4_2g29822 FvH4_2g29824 FvH4_2g29824 FvH4_2g29824 FvH4_2g29840 FvH4_2g29840 FvH4_2g29840 FvH4_5g00550 FvH4_5g00550
malus_domestica MD02G1032700.v1.1 MD08G1140900.v1.1 MD08G1141400.v1.1 MD15G1117800.v1.1 MD15G1117900.v1.1
prunus_persica Prupe.1G471200_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471400_v2.0.a1 Prupe.7G242900_v2.0.a1
pyrus_communis pycom02g02690 pycom08g11890
rosa_chinensis RchiOBHm_Chr2g0111741 RchiOBHm_Chr2g0145991 RchiOBHm_Chr6g0298851 RchiOBHm_Chr6g0298871 RchiOBHm_Chr6g0298891 RchiOBHm_Chr6g0298901 RchiOBHm_Chr6g0298911 RchiOBHm_Chr7g0201801 RchiOBHm_Chr7g0211531 RchiOBHm_Chr7g0214601
rosa_laevigata RLG00000002931 RLG00000003000 RLG00000003647 RLG00000011476 RLG00000011479
rosa_multiflora Rmu_sc0001030.1_g000012 Rmu_sc0001548.1_g000041 Rmu_ssc0000397.1_g000033 Rmu_ssc0000397.1_g000036 Rmu_ssc0000397.1_g000037 Rmu_ssc0000397.1_g000039 Rmu_ssc0000397.1_g000048
rosa_roxburghii Rroxscaffold_1G00003830 Rroxscaffold_3G00247810 Rroxscaffold_3G00255060 Rroxscaffold_7G00169220 Rroxscaffold_7G00169230 Rroxscaffold_7G00169280
rosa_rugosa Rorug02G0223600 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283200 Rorug06G0283300 Rorug06G0283400 Rorug06G0283500 Rorug07G0066000
rosa_samantha Rh2AG511300 Rh2CG235100 Rh2CG496400 Rh2DG330900 Rh6BG403100 Rh6BG403300 Rh6BG403400 Rh6BG403500 Rh6CG408700 Rh6CG408900 Rh6CG409000 Rh6CG409200 Rh6DG395800 Rh6DG395900 Rh6DG396000 Rh6DG396200 Rh7AG193400 Rh7BG195100 Rh7BG436600 Rh7DG199300 Rh7DG265500
rosa_wichuraiana Rw2G016900 Rw6G034570 Rw6G034580 Rw6G034590 Rw6G034600 Rw7G016900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 729, 1592
Acc65I GGTACC 1 cut(s) 3459
AccB1I GGYRCC 3 cut(s) 2222, 3260, 3459
AccB7I CCANNNNNTGG 3 cut(s) 128, 2618, 3061
AccIII TCCGGA 1 cut(s) 832
AciI CCGC 6 cut(s) 193, 425, 1608, 2129, 2898, 3333
AclI AACGTT 1 cut(s) 3137
AcoI YGGCCR 3 cut(s) 156, 1401, 2186
AcsI RAATTY 6 cut(s) 30, 224, 652, 1706, 1785, 1939
AcuI CTGAAG 3 cut(s) 437, 2183, 2877
AcvI CACGTG 1 cut(s) 788
AcyI GRCGYC 1 cut(s) 1511
AfiI CCNNNNNNNGG 9 cut(s) 128, 1545, 1867, 2415, 2618, 3002, 3061, 3589, 3612
AflII CTTAAG 1 cut(s) 1772
AflIII ACRYGT 1 cut(s) 345
AgeI ACCGGT 2 cut(s) 583, 2528
AhdI GACNNNNNGTC 1 cut(s) 488
AjnI CCWGG 6 cut(s) 1352, 2188, 2408, 2518, 2873, 3659
AloI GAACNNNNNNTCC 2 cut(s) 3585, 3617
Alw21I GWGCWC 3 cut(s) 801, 2506, 2577
Alw26I GTCTC 9 cut(s) 290, 437, 703, 903, 1326, 1669, 1720, 1782, 3690
AlwNI CAGNNNCTG 1 cut(s) 719
Ama87I CYCGRG 5 cut(s) 800, 1623, 1892, 3001, 3589
Aor13HI TCCGGA 1 cut(s) 832
AoxI GGCC 4 cut(s) 156, 1401, 2186, 2620
ApoI RAATTY 6 cut(s) 30, 224, 652, 1706, 1785, 1939
AsiGI ACCGGT 2 cut(s) 583, 2528
Asp700I GAANNNNTTC 2 cut(s) 3328, 3584
Asp718I GGTACC 1 cut(s) 3459
AspLEI GCGC 1 cut(s) 2550
AspS9I GGNCC 5 cut(s) 116, 581, 782, 2783, 3663
AsuC2I CCSGG 2 cut(s) 190, 2867
AsuHPI GGTGA 3 cut(s) 880, 2777, 3472
AsuII TTCGAA 1 cut(s) 28
AvaI CYCGRG 5 cut(s) 800, 1623, 1892, 3001, 3589
AvaII GGWCC 5 cut(s) 116, 581, 782, 2783, 3663
AxyI CCTNAGG 2 cut(s) 1540, 2427
BamHI GGATCC 2 cut(s) 1377, 1574
BanI GGYRCC 3 cut(s) 2222, 3260, 3459
BanII GRGCYC 2 cut(s) 2506, 2577
BarI GAAGNNNNNNTAC 4 cut(s) 213, 245, 3452, 3484
BbrPI CACGTG 1 cut(s) 788
BbsI GAAGAC 1 cut(s) 169
Bbv12I GWGCWC 3 cut(s) 801, 2506, 2577
BceAI ACGGC 2 cut(s) 1654, 3520
BcgI CGANNNNNNTGC 4 cut(s) 977, 1011, 2571, 2605
BciT130I CCWGG 6 cut(s) 1354, 2190, 2410, 2520, 2875, 3661
BciVI GTATCC 1 cut(s) 3752
BclI TGATCA 1 cut(s) 2811
BcnI CCSGG 2 cut(s) 190, 2867
BcoDI GTCTC 9 cut(s) 290, 437, 703, 903, 1326, 1669, 1720, 1782, 3690
BfaI CTAG 3 cut(s) 315, 431, 2631
BfmI CTRYAG 3 cut(s) 690, 1746, 3408
BfrI CTTAAG 1 cut(s) 1772
BfuI GTATCC 1 cut(s) 3752
BglII AGATCT 3 cut(s) 1986, 3013, 3199
BlpI GCTNAGC 1 cut(s) 1932
BmcAI AGTACT 2 cut(s) 896, 1911
Bme1390I CCNGG 8 cut(s) 190, 1354, 2190, 2410, 2520, 2867, 2875, 3661
Bme18I GGWCC 5 cut(s) 116, 581, 782, 2783, 3663
BmeRI GACNNNNNGTC 1 cut(s) 488
BmeT110I CYCGRG 5 cut(s) 800, 1623, 1892, 3001, 3589
BmgT120I GGNCC 5 cut(s) 116, 581, 782, 2783, 3663
BmrFI CCNGG 8 cut(s) 190, 1354, 2190, 2410, 2520, 2867, 2875, 3661
BmrI ACTGGG 2 cut(s) 907, 1669
BmsI GCATC 8 cut(s) 561, 1188, 1618, 2617, 2719, 2868, 2923, 3789
BmuI ACTGGG 2 cut(s) 907, 1669
BpiI GAAGAC 1 cut(s) 169
BplI GAGNNNNNCTC 2 cut(s) 1323, 1355
BpmI CTGGAG 3 cut(s) 692, 2903, 3734
Bpu1102I GCTNAGC 1 cut(s) 1932
Bpu14I TTCGAA 1 cut(s) 28
BpuEI CTTGAG 2 cut(s) 455, 1367
BpuMI CCSGG 2 cut(s) 190, 2867
Bsa29I ATCGAT 2 cut(s) 1178, 2591
BsaAI YACGTR 1 cut(s) 788
BsaBI GATNNNNATC 2 cut(s) 1391, 2456
BsaHI GRCGYC 1 cut(s) 1511
BsaI GGTCTC 3 cut(s) 437, 1326, 1669
BsaJI CCNNGG 7 cut(s) 816, 1353, 1755, 2688, 3000, 3175, 3516
BsaWI WCCGGW 3 cut(s) 583, 832, 2528
BsaXI ACNNNNNCTCC 6 cut(s) 2493, 2523, 3396, 3426, 3714, 3744
Bsc4I CCNNNNNNNGG 9 cut(s) 128, 1545, 1867, 2415, 2618, 3002, 3061, 3589, 3612
Bse118I RCCGGY 6 cut(s) 583, 617, 2225, 2528, 2780, 3274
Bse1I ACTGG 6 cut(s) 902, 958, 1664, 2272, 3089, 3717
Bse21I CCTNAGG 2 cut(s) 1540, 2427
Bse3DI GCAATG 5 cut(s) 2797, 2979, 3219, 3540, 3809
Bse8I GATNNNNATC 2 cut(s) 1391, 2456
BseAI TCCGGA 1 cut(s) 832
BseBI CCWGG 6 cut(s) 1354, 2190, 2410, 2520, 2875, 3661
BseCI ATCGAT 2 cut(s) 1178, 2591
BseDI CCNNGG 7 cut(s) 816, 1353, 1755, 2688, 3000, 3175, 3516
BseJI GATNNNNATC 2 cut(s) 1391, 2456
BseLI CCNNNNNNNGG 9 cut(s) 128, 1545, 1867, 2415, 2618, 3002, 3061, 3589, 3612
BseMI GCAATG 5 cut(s) 2797, 2979, 3219, 3540, 3809
BseMII CTCAG 7 cut(s) 248, 1224, 1916, 1923, 2418, 2532, 3279
BseNI ACTGG 6 cut(s) 902, 958, 1664, 2272, 3089, 3717
BseRI GAGGAG 2 cut(s) 695, 1847
BsgI GTGCAG 2 cut(s) 3135, 3290
Bsh1285I CGRYCG 1 cut(s) 2565
BshFI GGCC 4 cut(s) 158, 1403, 2188, 2622
BshNI GGYRCC 3 cut(s) 2222, 3260, 3459
BshTI ACCGGT 2 cut(s) 583, 2528
BshVI ATCGAT 2 cut(s) 1178, 2591
BsiEI CGRYCG 1 cut(s) 2565
BsiHKAI GWGCWC 3 cut(s) 801, 2506, 2577
BsiHKCI CYCGRG 5 cut(s) 800, 1623, 1892, 3001, 3589
BslFI GGGAC 1 cut(s) 102
BslI CCNNNNNNNGG 9 cut(s) 128, 1545, 1867, 2415, 2618, 3002, 3061, 3589, 3612
BsmAI GTCTC 9 cut(s) 290, 437, 703, 903, 1326, 1669, 1720, 1782, 3690
BsmBI CGTCTC 1 cut(s) 3690
BsmFI GGGAC 1 cut(s) 102
BsmI GAATGC 2 cut(s) 18, 3214
BsnI GGCC 4 cut(s) 158, 1403, 2188, 2622
Bso31I GGTCTC 3 cut(s) 437, 1326, 1669
BsoBI CYCGRG 5 cut(s) 800, 1623, 1892, 3001, 3589
Bsp119I TTCGAA 1 cut(s) 28
Bsp1286I GDGCHC 3 cut(s) 801, 2506, 2577
Bsp13I TCCGGA 1 cut(s) 832
Bsp1720I GCTNAGC 1 cut(s) 1932
Bsp19I CCATGG 2 cut(s) 816, 3516
BspACI CCGC 6 cut(s) 193, 425, 1608, 2129, 2898, 3333
BspANI GGCC 4 cut(s) 158, 1403, 2188, 2622
BspCNI CTCAG 7 cut(s) 247, 1223, 1915, 1924, 2419, 2533, 3278
BspDI ATCGAT 2 cut(s) 1178, 2591
BspEI TCCGGA 1 cut(s) 832
BspHI TCATGA 1 cut(s) 1107
BspMAI CTGCAG 1 cut(s) 694
BspT104I TTCGAA 1 cut(s) 28
BspT107I GGYRCC 3 cut(s) 2222, 3260, 3459
BspTI CTTAAG 1 cut(s) 1772
BspTNI GGTCTC 3 cut(s) 437, 1326, 1669
BsrDI GCAATG 5 cut(s) 2797, 2979, 3219, 3540, 3809
BsrFI RCCGGY 6 cut(s) 583, 617, 2225, 2528, 2780, 3274
BsrI ACTGG 6 cut(s) 902, 958, 1664, 2272, 3089, 3717
BssAI RCCGGY 6 cut(s) 583, 617, 2225, 2528, 2780, 3274
BssECI CCNNGG 7 cut(s) 816, 1353, 1755, 2688, 3000, 3175, 3516
BssNI GRCGYC 1 cut(s) 1511
BssT1I CCWWGG 4 cut(s) 816, 1755, 3175, 3516
Bst2UI CCWGG 6 cut(s) 1354, 2190, 2410, 2520, 2875, 3661
Bst6I CTCTTC 6 cut(s) 361, 602, 1106, 1346, 1996, 3590
BstACI GRCGYC 1 cut(s) 1511
BstAFI CTTAAG 1 cut(s) 1772
BstAPI GCANNNNNTGC 1 cut(s) 13
BstBAI YACGTR 1 cut(s) 788
BstBI TTCGAA 1 cut(s) 28
BstC8I GCNNGC 6 cut(s) 714, 996, 1564, 2227, 2332, 3067
BstDSI CCRYGG 3 cut(s) 816, 2688, 3516
BstENI CCTNNNNNAGG 1 cut(s) 1543
BstHHI GCGC 1 cut(s) 2550
BstMAI GTCTC 9 cut(s) 290, 437, 703, 903, 1326, 1669, 1720, 1782, 3690
BstMCI CGRYCG 1 cut(s) 2565
BstMWI GCNNNNNNNGC 7 cut(s) 13, 1409, 2554, 2636, 2799, 2906, 3845
BstNI CCWGG 6 cut(s) 1354, 2190, 2410, 2520, 2875, 3661
BstNSI RCATGY 3 cut(s) 349, 906, 1086
BstSCI CCNGG 8 cut(s) 188, 1352, 2188, 2408, 2518, 2865, 2873, 3659
BstSFI CTRYAG 3 cut(s) 690, 1746, 3408
BstV2I GAAGAC 1 cut(s) 169
BstX2I RGATCY 6 cut(s) 1377, 1386, 1574, 1986, 3013, 3199
BstXI CCANNNNNNTGG 2 cut(s) 1644, 2242
BstYI RGATCY 6 cut(s) 1377, 1386, 1574, 1986, 3013, 3199
Bsu15I ATCGAT 2 cut(s) 1178, 2591
Bsu36I CCTNAGG 2 cut(s) 1540, 2427
BsuI GTATCC 1 cut(s) 3752
BsuRI GGCC 4 cut(s) 158, 1403, 2188, 2622
BsuTUI ATCGAT 2 cut(s) 1178, 2591
BtgI CCRYGG 3 cut(s) 816, 2688, 3516
BtgZI GCGATG 2 cut(s) 1280, 3127
BtsI GCAGTG 1 cut(s) 1583
BtsIMutI CAGTG 8 cut(s) 294, 1218, 1583, 2224, 2664, 2676, 3082, 3273
Cac8I GCNNGC 6 cut(s) 714, 996, 1564, 2227, 2332, 3067
CaiI CAGNNNCTG 1 cut(s) 719
CciI TCATGA 1 cut(s) 1107
CfoI GCGC 1 cut(s) 2550
Cfr10I RCCGGY 6 cut(s) 583, 617, 2225, 2528, 2780, 3274
Cfr13I GGNCC 5 cut(s) 116, 581, 782, 2783, 3663
ClaI ATCGAT 2 cut(s) 1178, 2591
CseI GACGC 1 cut(s) 1500
CsiI ACCWGGT 1 cut(s) 2518
CspAI ACCGGT 2 cut(s) 583, 2528
DraI TTTAAA 1 cut(s) 180
DriI GACNNNNNGTC 1 cut(s) 488
EaeI YGGCCR 3 cut(s) 156, 1401, 2186
Eam1104I CTCTTC 6 cut(s) 361, 602, 1106, 1346, 1996, 3590
Eam1105I GACNNNNNGTC 1 cut(s) 488
EarI CTCTTC 6 cut(s) 361, 602, 1106, 1346, 1996, 3590
EciI GGCGGA 1 cut(s) 1623
Ecl136II GAGCTC 2 cut(s) 2504, 2575
Eco130I CCWWGG 4 cut(s) 816, 1755, 3175, 3516
Eco24I GRGCYC 2 cut(s) 2506, 2577
Eco31I GGTCTC 3 cut(s) 437, 1326, 1669
Eco32I GATATC 1 cut(s) 2362
Eco47I GGWCC 5 cut(s) 116, 581, 782, 2783, 3663
Eco53kI GAGCTC 2 cut(s) 2504, 2575
Eco57I CTGAAG 3 cut(s) 437, 2183, 2877
Eco72I CACGTG 1 cut(s) 788
Eco81I CCTNAGG 2 cut(s) 1540, 2427
Eco88I CYCGRG 5 cut(s) 800, 1623, 1892, 3001, 3589
EcoICRI GAGCTC 2 cut(s) 2504, 2575
EcoNI CCTNNNNNAGG 1 cut(s) 1543
EcoO109I RGGNCCY 1 cut(s) 116
EcoRII CCWGG 6 cut(s) 1352, 2188, 2408, 2518, 2873, 3659
EcoRV GATATC 1 cut(s) 2362
EcoT14I CCWWGG 4 cut(s) 816, 1755, 3175, 3516
EcoT22I ATGCAT 1 cut(s) 1203
EcoT38I GRGCYC 2 cut(s) 2506, 2577
ErhI CCWWGG 4 cut(s) 816, 1755, 3175, 3516
Esp3I CGTCTC 1 cut(s) 3690
FalI AAGNNNNNCTT 2 cut(s) 2164, 2196
FaqI GGGAC 1 cut(s) 102
FauI CCCGC 1 cut(s) 186
FauNDI CATATG 2 cut(s) 827, 1456
FbaI TGATCA 1 cut(s) 2811
FriOI GRGCYC 2 cut(s) 2506, 2577
FspBI CTAG 3 cut(s) 315, 431, 2631
GlaI GCGC 1 cut(s) 2549
GsuI CTGGAG 3 cut(s) 692, 2903, 3734
HaeIII GGCC 4 cut(s) 158, 1403, 2188, 2622
HgaI GACGC 1 cut(s) 1500
HhaI GCGC 1 cut(s) 2550
Hin1I GRCGYC 1 cut(s) 1511
Hin6I GCGC 1 cut(s) 2548
HinP1I GCGC 1 cut(s) 2548
HincII GTYRAC 3 cut(s) 1060, 2538, 2923
HindII GTYRAC 3 cut(s) 1060, 2538, 2923
HindIII AAGCTT 1 cut(s) 951
HpaI GTTAAC 2 cut(s) 1060, 2923
HphI GGTGA 3 cut(s) 880, 2777, 3472
Hpy166II GTNNAC 8 cut(s) 785, 1060, 1231, 2273, 2538, 2674, 2923, 3132
Hpy8I GTNNAC 8 cut(s) 785, 1060, 1231, 2273, 2538, 2674, 2923, 3132
Hpy99I CGWCG 1 cut(s) 992
HpyCH4IV ACGT 3 cut(s) 787, 3137, 3699
HpyF10VI GCNNNNNNNGC 7 cut(s) 13, 1409, 2554, 2636, 2799, 2906, 3845
HpySE526I ACGT 3 cut(s) 787, 3137, 3699
Hsp92I GRCGYC 1 cut(s) 1511
HspAI GCGC 1 cut(s) 2548
Kpn2I TCCGGA 1 cut(s) 832
KpnI GGTACC 1 cut(s) 3463
KroI GCCGGC 1 cut(s) 2225
KroNI GCCGGC 1 cut(s) 2227
Ksp22I TGATCA 1 cut(s) 2811
KspAI GTTAAC 2 cut(s) 1060, 2923
LmnI GCTCC 7 cut(s) 1238, 2418, 2482, 2501, 2798, 2807, 3404
LweI GCATC 8 cut(s) 561, 1188, 1618, 2617, 2719, 2868, 2923, 3789
MabI ACCWGGT 1 cut(s) 2518
MaeI CTAG 3 cut(s) 315, 431, 2631
MaeII ACGT 3 cut(s) 787, 3137, 3699
MfeI CAATTG 1 cut(s) 969
MflI RGATCY 6 cut(s) 1377, 1386, 1574, 1986, 3013, 3199
MhlI GDGCHC 3 cut(s) 801, 2506, 2577
MlyI GAGTC 6 cut(s) 97, 564, 1202, 2202, 2223, 3725
MmeI TCCRAC 3 cut(s) 310, 559, 1703
Mph1103I ATGCAT 1 cut(s) 1203
MroI TCCGGA 1 cut(s) 832
MroNI GCCGGC 1 cut(s) 2225
MroXI GAANNNNTTC 2 cut(s) 3328, 3584
MseI TTAA 9 cut(s) 179, 1059, 1272, 1773, 2207, 2850, 2922, 2987, 3852
MslI CAYNNNNRTG 3 cut(s) 350, 2108, 2240
MspA1I CMGCKG 2 cut(s) 425, 3320
MspCI CTTAAG 1 cut(s) 1772
MspR9I CCNGG 8 cut(s) 190, 1354, 2190, 2410, 2520, 2867, 2875, 3661
MunI CAATTG 1 cut(s) 969
Mva1269I GAATGC 2 cut(s) 18, 3214
MvaI CCWGG 6 cut(s) 1354, 2190, 2410, 2520, 2875, 3661
MwoI GCNNNNNNNGC 7 cut(s) 13, 1409, 2554, 2636, 2799, 2906, 3845
NaeI GCCGGC 1 cut(s) 2227
NciI CCSGG 2 cut(s) 190, 2867
NcoI CCATGG 2 cut(s) 816, 3516
NdeI CATATG 2 cut(s) 827, 1456
NgoMIV GCCGGC 1 cut(s) 2225
NmeAIII GCCGAG 2 cut(s) 290, 963
NmuCI GTSAC 3 cut(s) 556, 868, 2377
NsiI ATGCAT 1 cut(s) 1203
NspI RCATGY 3 cut(s) 349, 906, 1086
NspV TTCGAA 1 cut(s) 28
PaeR7I CTCGAG 2 cut(s) 800, 1623
PagI TCATGA 1 cut(s) 1107
PciI ACATGT 1 cut(s) 345
PcsI WCGNNNNNNNCGW 2 cut(s) 2733, 3617
PctI GAATGC 2 cut(s) 18, 3214
PdiI GCCGGC 1 cut(s) 2227
PdmI GAANNNNTTC 2 cut(s) 3328, 3584
PflMI CCANNNNNTGG 3 cut(s) 128, 2618, 3061
PinAI ACCGGT 2 cut(s) 583, 2528
Ple19I CGATCG 1 cut(s) 2565
PleI GAGTC 6 cut(s) 97, 563, 1202, 2201, 2222, 3724
PmaCI CACGTG 1 cut(s) 788
PmlI CACGTG 1 cut(s) 788
PpsI GAGTC 6 cut(s) 97, 563, 1202, 2201, 2222, 3724
Ppu21I YACGTR 1 cut(s) 788
PpuMI RGGWCCY 1 cut(s) 116
PscI ACATGT 1 cut(s) 345
PsiI TTATAA 2 cut(s) 729, 1592
Psp124BI GAGCTC 2 cut(s) 2506, 2577
Psp1406I AACGTT 1 cut(s) 3137
Psp5II RGGWCCY 1 cut(s) 116
Psp6I CCWGG 6 cut(s) 1352, 2188, 2408, 2518, 2873, 3659
PspCI CACGTG 1 cut(s) 788
PspGI CCWGG 6 cut(s) 1352, 2188, 2408, 2518, 2873, 3659
PspPI GGNCC 5 cut(s) 116, 581, 782, 2783, 3663
PspPPI RGGWCCY 1 cut(s) 116
PspXI VCTCGAGB 1 cut(s) 800
PstI CTGCAG 1 cut(s) 694
PstNI CAGNNNCTG 1 cut(s) 719
PsuI RGATCY 6 cut(s) 1377, 1386, 1574, 1986, 3013, 3199
PvuI CGATCG 1 cut(s) 2565
PvuII CAGCTG 1 cut(s) 3320
RseI CAYNNNNRTG 3 cut(s) 350, 2108, 2240
SacI GAGCTC 2 cut(s) 2506, 2577
SaqAI TTAA 9 cut(s) 179, 1059, 1272, 1773, 2207, 2850, 2922, 2987, 3852
Sau96I GGNCC 5 cut(s) 116, 581, 782, 2783, 3663
ScaI AGTACT 2 cut(s) 896, 1911
SchI GAGTC 6 cut(s) 97, 564, 1202, 2202, 2223, 3725
ScrFI CCNGG 8 cut(s) 190, 1354, 2190, 2410, 2520, 2867, 2875, 3661
SduI GDGCHC 3 cut(s) 801, 2506, 2577
SexAI ACCWGGT 1 cut(s) 2518
SfaNI GCATC 8 cut(s) 561, 1188, 1618, 2617, 2719, 2868, 2923, 3789
SfcI CTRYAG 3 cut(s) 690, 1746, 3408
Sfr274I CTCGAG 2 cut(s) 800, 1623
SfuI TTCGAA 1 cut(s) 28
SinI GGWCC 5 cut(s) 116, 581, 782, 2783, 3663
SlaI CTCGAG 2 cut(s) 800, 1623
SmiMI CAYNNNNRTG 3 cut(s) 350, 2108, 2240
SmlI CTYRAG 5 cut(s) 470, 800, 1382, 1623, 1772
SmoI CTYRAG 5 cut(s) 470, 800, 1382, 1623, 1772
SsiI CCGC 6 cut(s) 193, 425, 1608, 2129, 2898, 3333
SspMI CTAG 3 cut(s) 315, 431, 2631
SstI GAGCTC 2 cut(s) 2506, 2577
StyD4I CCNGG 8 cut(s) 188, 1352, 2188, 2408, 2518, 2865, 2873, 3659
StyI CCWWGG 4 cut(s) 816, 1755, 3175, 3516
TaiI ACGT 3 cut(s) 790, 3140, 3702
TatI WGTACW 6 cut(s) 894, 1479, 1486, 1909, 3474, 3489
TauI GCSGC 2 cut(s) 428, 2900
Tru1I TTAA 9 cut(s) 179, 1059, 1272, 1773, 2207, 2850, 2922, 2987, 3852
Tru9I TTAA 9 cut(s) 179, 1059, 1272, 1773, 2207, 2850, 2922, 2987, 3852
TscAI CASTG 8 cut(s) 294, 1218, 1590, 2224, 2664, 2676, 3089, 3273
TseFI GTSAC 3 cut(s) 556, 868, 2377
Tsp45I GTSAC 3 cut(s) 556, 868, 2377
TspGWI ACGGA 3 cut(s) 222, 1290, 3417
TspRI CASTG 8 cut(s) 294, 1218, 1590, 2224, 2664, 2676, 3089, 3273
Van91I CCANNNNNTGG 3 cut(s) 128, 2618, 3061
Vha464I CTTAAG 1 cut(s) 1772
VpaK11BI GGWCC 5 cut(s) 116, 581, 782, 2783, 3663
XagI CCTNNNNNAGG 1 cut(s) 1543
XapI RAATTY 6 cut(s) 30, 224, 652, 1706, 1785, 1939
XbaI TCTAGA 1 cut(s) 430
XceI RCATGY 3 cut(s) 349, 906, 1086
XcmI CCANNNNNNNNNTGG 5 cut(s) 145, 680, 1511, 1924, 3058
XhoI CTCGAG 2 cut(s) 800, 1623
XmnI GAANNNNTTC 2 cut(s) 3328, 3584
XspI CTAG 3 cut(s) 315, 431, 2631
ZrmI AGTACT 2 cut(s) 896, 1911
Zsp2I ATGCAT 1 cut(s) 1203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.