Rroxscaffold_3G00255060
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000493
Physical Location & Seq
Forward (+)
49330839 .. 49339556
8718 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00255060.1

Sequence Viewer

Length: 3588 bp
ATGGATGAGTTTGTATCAGAGAGGACATCAGCCTATGTCAGTCAACATGATCTCCACATACCAGAATTGACAGTGAGAGAAACACTGGCTTTTGCAGCTAGATGTCAAGGTGTTGGACCACGTTATGAAATGTTGGTGGAATTACTAAGGAGAGAAAAGGCTGCAAGTATTATGCCAGATCTTGATCTTGATCTCTACATGAAGGCAGCAACACTAGAAGGACAGGAAACTAATATTGTTACAGACTTTATTCTCAAGGTTTTGGGACTCGAAGATTGTGCTGAAATTATGGTAGGAGATGAAATGACCAGAGGTATCTCTGGTGGACAAAAAAAGCGACTCACCACAGGGGAGATGCTGGTTGGACCAGAAAGAGTACTTCTCATGGATGAGATATCAAACGGGCTGGACAGTTCAACAACATTGCAAATAGTGAAATCGCTACAACAATACGTCCACATCCTCAACGGAACTGCACTAATCGCTCTACTGCAGCCAGCACCAGAGACTTATGATCTCTTCGATGATATAATTCTCCTTTCGGATGGCTACATTGTGTATCAAGGTCCCCGTGAGAATGTTCTTGAGTTCTTTGAGTACATGGGCTTCAAATGTCCAGAGCGGAAAGGAGTTGCTGATTTCCTACAAGAGGTGACATCAAGAAAAGATCAAGAGCAATACTGGGCTCATATGGATAGGCCTTATAGCTTTGTAACAACCAAAGAATTTTCTGAAGCAATGCAGTTATTTCACATTGGTAGAAAACTTGGTGATGAGCTTTCTGTTCCATTTAACAAGTCAGAAAGCTGCCCTGGTGCTTTGTCAACTAAAAAATATGGTGTTAGTAAGAAGGAATTGTTCAAAGCTTGTATGGCTAGACAAATTTTGCTTACAAAGAGGAATTCATTTGTCTACATTTTCAAATTGGTGCAGCTTATTCTAGTTGCTGTTACAACAATGACACTATTTTTTCATACTGAGATGGAGCGAAACACAGTTGCAGATGGTAGGATTTATATGGGAGCTTTATTCTATACGCTACTCGCAATTATGCTTAATGGGTTTGCAGAGCTTCATATGACAGTCACTAGACTGCCTGTGTTTTTCAAGCAAAGGGACCATCTGTTCTATCCTGCTTGGGCATACTCTTTACCCCCATGGCTGATAAGGATCCCTATAACTTTTGTGGACGTTTTCATCTGGGTGATCATTACTTACTACAGTGTAGGTTATGATCCGAGCGTTGAAAGGTTTTTCAAACAGTTCCTTCTACTGCTGTGTATTAGCCAGATGGCAAATGGACTGTTTCGATTGATAGGGGCACTAGGAAGGAACATAACTATTGCAAATACATTTGGATTTGGTACTGTACTTGTCATTCTTGGTCTGTGTGGCTTTATCATATCAAAAGAGGATTTGAAGAAATGGTTGTTGTGGGGATATTGGCTCTCACCAATCACGTATGGACTGAATGCCATTGCTGTCAATGAATTTCTCGGAAAAAGTTGGAGACATGTTCCTGCCAACTCAACAGAAGCATTAGGAGTTATGGTCTTGAAGTATCAAGGCATATTCCCAGACGCACGCTTCTACTGGATTGGATTAGTAGCTTTGATGGGATTTATTCTTCTGCTGAATTTCCTTTTCACATTGGCACTTCAGTATCTTGATCCTCTTGAGATGCCCCAAGCAGTACTATCCAATGAGGCATCCTCTACAACAGAGGATAGCAGAGTTGCAAGCAATCCTACTATGCAGCATGGCATGGTTCTTCCATTTGAACCCCTTTCAATTACTTTCGAGGAAATCAGATATGCTGTTGATACGCCTGAGAAAATGAAAGATCAAAGTGAAACTCGAGATCGGCTAGAACTTCTGAAGGGTGTGAGTGGTACTTTTAGGCCTGGAGTTCTAACAGCTTTGATGGGTGTTAGTGGTGCTGGGAAAACCACTCTCATGGATGTCTTGGCAGGAAGGAAGACAAGTGGATATATTGAGGGGAGGATCACCTTATCTGGCTATGCAAAAAGGCAAGAAACATTTGCTCGCATATCAGGATACTGTGAGCAAATAGATATACACTCCCCTCATGTCACAGTTTACGAGTCTTTGGTTTACTCTGCATGGCTCCGGTTGCCCCGGGAGGTTGATTCCCCAACTAGAAAGATGTTTATTGAAGAAGTCATGGAGCTTGTAGAAATGACCTCAATAAGTGACAGACTTGTTGGATTGCCAGGAGTGAATGGTCTCTCAACTGAACAACGAAAAAGGATAACAATTGCAGTAGAGCTTGTTGCCAACCCCTCGATAATATTTCTGGATGAGCCGACTTCTGGCCTCGATGCTAGGGCAGCAGCAATTGTAATGAGAACGGTGAGGAATACAGTGGACACTGGGAGAACTGTAGTCTGTACCATCCACCAACCAAGCATTGATATATTTGATGCTTTTGATGAGATGTTGCTTCTGAAACAGGGAGGCGAAGAAATATATGTTGGCCCTTTAGGCCACCATTCTTCCCAGATGATTGAATACTTTGAGGAGATTAGTGGAGTTTCTAAAATCAAGGATGGTTACAATCCAGCAACCTGGATGTTGGAGGTTACTTCTCCAGCACAAGAAGCATCTCTTGGGGTTGATTTTGCCAACATATACAAGAATTCAGAAATATATAGGACAAATAAGGCATTGATAAAGGAACTAAGTACTCCTGCACCAAATTCAAAGGACTTGTACTTCCCAACACAATATTCGCAGTCCTTCTTTACCCAGTGTCTAGCATGCCTATGGAAACAGAGTGTATCATATTGGCGAAACCCTCAATACACTGCAGTGAGACTTCTTTTCACAGCTATGATGGCCGGACTACTTGGGTCATTTTTTTGGGATCTTGGCTCCAAAAGACAAAAGCAAAGAGATCTCTTTTGTGCGATGGGTTCTATGTATGCTGCTGTGCTCTTTATTGGTATACAAAATTCCTTGTCAGTGCAGCCAATCGTAGGTGTTGAGAGGTTGGTCTCTTACAGAGAAACGGCTGCTGGAATGTACTCAGCTTTTCCATTTGCCTTTGGACAGGTTATCATTGAGATCCCATACACTTTGATCCAGACTATTATATTTGGGGTTATAGCATACTCCATGGTCGGATTTGATTGGACAGTCAGCAAGTTCTTTTGTTATCTCTTGTTCATGTACCTCACCTTCTTATACTTCACCTTCCATGGCATGATGATTGTGGCCATTACTCCCAACAACACCATCTCTGCTGTAGTTTCGTCTGCCTTCTATCCATTATGGAATGTTATTTCAGGATATCTCATTCCCAAAACAAGAATTCCAATATGGTGGAGATGGTTCTATTGGGTCAGCCCGACCTCTTGGAGCTTGTATGGATTGTTTTCTTCACAGTTTGGAGGTCTCAAGGACACACTTGATTCTGGTGAAACTGTAGATCATTTTATGAGGACATATTTTGGATATAGGAAAGACTTTCTAGGTGTTGTTGTAGCTGTGCTTGTTGGGTTTTCAGCGTTCTTTGTTTTCATCTTTGCTTTTGCAGTCAAGAAACTAAACTTCCAAAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1195

Amino Acids

135.38

Weight (kDa)

6.04

Isoelectric Point (pI)

37.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 8 - 132 8.4e-06 ABC transporter
ABC2_membrane_7 PF19055 165 - 216 9.9e-06 ABC-2 type transporter
ABC2_membrane PF01061 286 - 498 3.6e-48 ABC-2 type transporter
PDR_assoc PF08370 503 - 565 1.3e-22 Plant PDR ABC transporter associated
ABC_tran PF00005 626 - 778 2.2e-21 ABC transporter
ABC2_membrane PF01061 923 - 1137 1.6e-53 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000493)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29820 FvH4_2g29820 FvH4_2g29822 FvH4_2g29824 FvH4_2g29824 FvH4_2g29824 FvH4_2g29840 FvH4_2g29840 FvH4_2g29840 FvH4_5g00550 FvH4_5g00550
malus_domestica MD02G1032700.v1.1 MD08G1140900.v1.1 MD08G1141400.v1.1 MD15G1117800.v1.1 MD15G1117900.v1.1
prunus_persica Prupe.1G471200_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471400_v2.0.a1 Prupe.7G242900_v2.0.a1
pyrus_communis pycom02g02690 pycom08g11890
rosa_chinensis RchiOBHm_Chr2g0111741 RchiOBHm_Chr2g0145991 RchiOBHm_Chr6g0298851 RchiOBHm_Chr6g0298871 RchiOBHm_Chr6g0298891 RchiOBHm_Chr6g0298901 RchiOBHm_Chr6g0298911 RchiOBHm_Chr7g0201801 RchiOBHm_Chr7g0211531 RchiOBHm_Chr7g0214601
rosa_laevigata RLG00000002931 RLG00000003000 RLG00000003647 RLG00000011476 RLG00000011479
rosa_multiflora Rmu_sc0001030.1_g000012 Rmu_sc0001548.1_g000041 Rmu_ssc0000397.1_g000033 Rmu_ssc0000397.1_g000036 Rmu_ssc0000397.1_g000037 Rmu_ssc0000397.1_g000039 Rmu_ssc0000397.1_g000048
rosa_roxburghii Rroxscaffold_1G00003830 Rroxscaffold_3G00247810 Rroxscaffold_3G00255060 Rroxscaffold_7G00169220 Rroxscaffold_7G00169230 Rroxscaffold_7G00169280
rosa_rugosa Rorug02G0223600 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283200 Rorug06G0283300 Rorug06G0283400 Rorug06G0283500 Rorug07G0066000
rosa_samantha Rh2AG511300 Rh2CG235100 Rh2CG496400 Rh2DG330900 Rh6BG403100 Rh6BG403300 Rh6BG403400 Rh6BG403500 Rh6CG408700 Rh6CG408900 Rh6CG409000 Rh6CG409200 Rh6DG395800 Rh6DG395900 Rh6DG396000 Rh6DG396200 Rh7AG193400 Rh7BG195100 Rh7BG436600 Rh7DG199300 Rh7DG265500
rosa_wichuraiana Rw2G016900 Rw6G034570 Rw6G034580 Rw6G034590 Rw6G034600 Rw7G016900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 622
AccI GTMKAC 2 cut(s) 912, 2968
AciI CCGC 1 cut(s) 622
AclWI GGATC 8 cut(s) 1165, 1178, 1229, 1664, 2012, 2895, 3082, 3097
AcoI YGGCCR 2 cut(s) 2859, 3237
AcsI RAATTY 9 cut(s) 725, 882, 901, 1490, 1636, 2659, 2719, 2974, 3333
AcuI CTGAAG 3 cut(s) 753, 1643, 1898
AfiI CCNNNNNNNGG 3 cut(s) 649, 2333, 2999
AflIII ACRYGT 1 cut(s) 1513
AjnI CCWGG 4 cut(s) 811, 1903, 2233, 2588
AjuI GAANNNNNNNTTGG 6 cut(s) 2478, 2510, 2613, 2645, 2734, 2766
AleI CACNNNNGTG 1 cut(s) 2831
AloI GAACNNNNNNTCC 2 cut(s) 1109, 1141
Alw21I GWGCWC 1 cut(s) 2958
Alw26I GTCTC 6 cut(s) 500, 1504, 2252, 2830, 3022, 3422
AlwI GGATC 8 cut(s) 1165, 1178, 1229, 1664, 2012, 2895, 3082, 3097
Ama87I CYCGRG 2 cut(s) 1857, 2139
AoxI GGCC 7 cut(s) 698, 1901, 2335, 2497, 2506, 2859, 3237
ApoI RAATTY 9 cut(s) 725, 882, 901, 1490, 1636, 2659, 2719, 2974, 3333
AspS9I GGNCC 5 cut(s) 116, 365, 566, 1117, 2498
AsuC2I CCSGG 2 cut(s) 2140, 2141
AvaI CYCGRG 2 cut(s) 1857, 2139
AvaII GGWCC 4 cut(s) 116, 365, 566, 1117
BaeGI GKGCMC 1 cut(s) 1324
BaeI ACNNNNGTAYC 2 cut(s) 1646, 1679
BalI TGGCCA 1 cut(s) 3239
BamHI GGATCC 1 cut(s) 1170
BanII GRGCYC 1 cut(s) 688
BarI GAAGNNNNNNTAC 4 cut(s) 590, 622, 3185, 3217
BbsI GAAGAC 1 cut(s) 1985
Bbv12I GWGCWC 1 cut(s) 2958
BceAI ACGGC 1 cut(s) 3048
BcgI CGANNNNNNTGC 2 cut(s) 260, 294
BciT130I CCWGG 4 cut(s) 813, 1905, 2235, 2590
BciVI GTATCC 1 cut(s) 2051
BclI TGATCA 1 cut(s) 1206
BcnI CCSGG 2 cut(s) 2140, 2141
BcoDI GTCTC 6 cut(s) 500, 1504, 2252, 2830, 3022, 3422
BfmI CTRYAG 6 cut(s) 491, 1219, 2403, 2829, 3267, 3447
BfuI GTATCC 1 cut(s) 2051
BglI GCCNNNNNGGC 1 cut(s) 2505
BglII AGATCT 2 cut(s) 178, 2917
BmcAI AGTACT 3 cut(s) 378, 1695, 2707
Bme1390I CCNGG 6 cut(s) 813, 1905, 2140, 2141, 2235, 2590
Bme18I GGWCC 4 cut(s) 116, 365, 566, 1117
BmeT110I CYCGRG 2 cut(s) 1857, 2139
BmgT120I GGNCC 5 cut(s) 116, 365, 566, 1117, 2498
BmiI GGNNCC 5 cut(s) 568, 1118, 1172, 2129, 2896
BmrFI CCNGG 6 cut(s) 813, 1905, 2140, 2141, 2235, 2590
BmrI ACTGGG 3 cut(s) 691, 2403, 2764
BmsI GCATC 6 cut(s) 345, 1671, 1718, 2332, 2434, 2633
BmuI ACTGGG 3 cut(s) 691, 2403, 2764
BpiI GAAGAC 1 cut(s) 1985
BplI GAGNNNNNCTC 4 cut(s) 366, 398, 1697, 1729
BpmI CTGGAG 2 cut(s) 1926, 2595
BpuEI CTTGAG 4 cut(s) 239, 605, 1697, 3404
BpuMI CCSGG 2 cut(s) 2140, 2141
BsaAI YACGTR 1 cut(s) 1461
BsaBI GATNNNNATC 3 cut(s) 183, 189, 1169
BsaI GGTCTC 3 cut(s) 2252, 3022, 3422
BsaJI CCNNGG 6 cut(s) 811, 1157, 2138, 2139, 3138, 3220
BsaWI WCCGGW 1 cut(s) 2130
BsaXI ACNNNNNCTCC 4 cut(s) 36, 66, 1536, 1566
Bsc4I CCNNNNNNNGG 3 cut(s) 649, 2333, 2999
Bse1I ACTGG 5 cut(s) 90, 686, 1598, 2398, 2770
Bse3DI GCAATG 3 cut(s) 422, 744, 1476
Bse8I GATNNNNATC 3 cut(s) 183, 189, 1169
BseBI CCWGG 4 cut(s) 813, 1905, 2235, 2590
BseDI CCNNGG 6 cut(s) 811, 1157, 2138, 2139, 3138, 3220
BseJI GATNNNNATC 3 cut(s) 183, 189, 1169
BseLI CCNNNNNNNGG 3 cut(s) 649, 2333, 2999
BseMI GCAATG 3 cut(s) 422, 744, 1476
BseMII CTCAG 3 cut(s) 969, 1821, 3063
BseNI ACTGG 5 cut(s) 90, 686, 1598, 2398, 2770
BseRI GAGGAG 1 cut(s) 2555
BseSI GKGCMC 1 cut(s) 1324
BseYI CCCAGC 1 cut(s) 1940
BsgI GTGCAG 4 cut(s) 459, 950, 2697, 3008
BshFI GGCC 7 cut(s) 700, 1903, 2337, 2499, 2508, 2861, 3239
BsiHKAI GWGCWC 1 cut(s) 2958
BsiHKCI CYCGRG 2 cut(s) 1857, 2139
BsiSI CCGG 3 cut(s) 2131, 2140, 2862
BslFI GGGAC 3 cut(s) 279, 552, 1130
BslI CCNNNNNNNGG 3 cut(s) 649, 2333, 2999
BsmAI GTCTC 6 cut(s) 500, 1504, 2252, 2830, 3022, 3422
BsmFI GGGAC 3 cut(s) 279, 552, 1130
BsmI GAATGC 1 cut(s) 1477
BsnI GGCC 7 cut(s) 700, 1903, 2337, 2499, 2508, 2861, 3239
Bso31I GGTCTC 3 cut(s) 2252, 3022, 3422
BsoBI CYCGRG 2 cut(s) 1857, 2139
Bsp1286I GDGCHC 3 cut(s) 688, 1324, 2958
Bsp19I CCATGG 3 cut(s) 1157, 3138, 3220
BspACI CCGC 1 cut(s) 622
BspANI GGCC 7 cut(s) 700, 1903, 2337, 2499, 2508, 2861, 3239
BspCNI CTCAG 3 cut(s) 970, 1822, 3062
BspLI GGNNCC 5 cut(s) 568, 1118, 1172, 2129, 2896
BspMAI CTGCAG 2 cut(s) 495, 2833
BspPI GGATC 8 cut(s) 1165, 1178, 1229, 1664, 2012, 2895, 3082, 3097
BspTNI GGTCTC 3 cut(s) 2252, 3022, 3422
BsrBI CCGCTC 1 cut(s) 622
BsrDI GCAATG 3 cut(s) 422, 744, 1476
BsrI ACTGG 5 cut(s) 90, 686, 1598, 2398, 2770
BssECI CCNNGG 6 cut(s) 811, 1157, 2138, 2139, 3138, 3220
BssNAI GTATAC 1 cut(s) 2969
BssT1I CCWWGG 3 cut(s) 1157, 3138, 3220
Bst1107I GTATAC 1 cut(s) 2969
Bst2UI CCWGG 4 cut(s) 813, 1905, 2235, 2590
Bst6I CTCTTC 1 cut(s) 524
BstBAI YACGTR 1 cut(s) 1461
BstC8I GCNNGC 5 cut(s) 498, 1585, 1741, 2047, 2782
BstDEI CTNAG 5 cut(s) 146, 978, 1830, 2702, 3049
BstDSI CCRYGG 3 cut(s) 1157, 3138, 3220
BstENI CCTNNNNNAGG 1 cut(s) 647
BstMAI GTCTC 6 cut(s) 500, 1504, 2252, 2830, 3022, 3422
BstMWI GCNNNNNNNGC 8 cut(s) 95, 482, 872, 2134, 2351, 2505, 2621, 2858
BstNI CCWGG 4 cut(s) 813, 1905, 2235, 2590
BstNSI RCATGY 2 cut(s) 1517, 2784
BstSCI CCNGG 6 cut(s) 811, 1903, 2138, 2139, 2233, 2588
BstSFI CTRYAG 6 cut(s) 491, 1219, 2403, 2829, 3267, 3447
BstSLI GKGCMC 1 cut(s) 1324
BstV2I GAAGAC 1 cut(s) 1985
BstX2I RGATCY 5 cut(s) 178, 1170, 2887, 2917, 3087
BstXI CCANNNNNNTGG 3 cut(s) 1957, 2589, 3345
BstYI RGATCY 5 cut(s) 178, 1170, 2887, 2917, 3087
BstZ17I GTATAC 1 cut(s) 2969
BsuI GTATCC 1 cut(s) 2051
BsuRI GGCC 7 cut(s) 700, 1903, 2337, 2499, 2508, 2861, 3239
BtgI CCRYGG 3 cut(s) 1157, 3138, 3220
BtgZI GCGATG 1 cut(s) 2945
BtsI GCAGTG 2 cut(s) 2826, 2838
BtsIMutI CAGTG 9 cut(s) 78, 83, 1228, 2391, 2391, 2777, 2826, 2838, 2991
Cac8I GCNNGC 5 cut(s) 498, 1585, 1741, 2047, 2782
Cfr13I GGNCC 5 cut(s) 116, 365, 566, 1117, 2498
Cfr9I CCCGGG 1 cut(s) 2139
CseI GACGC 1 cut(s) 1589
DdeI CTNAG 5 cut(s) 146, 978, 1830, 2702, 3049
EaeI YGGCCR 2 cut(s) 2859, 3237
Eam1104I CTCTTC 1 cut(s) 524
EarI CTCTTC 1 cut(s) 524
Eco130I CCWWGG 3 cut(s) 1157, 3138, 3220
Eco147I AGGCCT 2 cut(s) 700, 1903
Eco24I GRGCYC 1 cut(s) 688
Eco31I GGTCTC 3 cut(s) 2252, 3022, 3422
Eco32I GATATC 2 cut(s) 396, 3314
Eco47I GGWCC 4 cut(s) 116, 365, 566, 1117
Eco57I CTGAAG 3 cut(s) 753, 1643, 1898
Eco88I CYCGRG 2 cut(s) 1857, 2139
EcoNI CCTNNNNNAGG 1 cut(s) 647
EcoO109I RGGNCCY 1 cut(s) 566
EcoRI GAATTC 3 cut(s) 901, 2659, 3333
EcoRII CCWGG 4 cut(s) 811, 1903, 2233, 2588
EcoRV GATATC 2 cut(s) 396, 3314
EcoT14I CCWWGG 3 cut(s) 1157, 3138, 3220
EcoT38I GRGCYC 1 cut(s) 688
ErhI CCWWGG 3 cut(s) 1157, 3138, 3220
FalI AAGNNNNNCTT 2 cut(s) 2613, 2645
FaqI GGGAC 3 cut(s) 279, 552, 1130
FauNDI CATATG 2 cut(s) 690, 1077
FbaI TGATCA 1 cut(s) 1206
FblI GTMKAC 2 cut(s) 912, 2968
FriOI GRGCYC 1 cut(s) 688
GsaI CCCAGC 1 cut(s) 1944
GsuI CTGGAG 2 cut(s) 1926, 2595
HaeIII GGCC 7 cut(s) 700, 1903, 2337, 2499, 2508, 2861, 3239
HapII CCGG 3 cut(s) 2131, 2140, 2862
HgaI GACGC 1 cut(s) 1589
HincII GTYRAC 2 cut(s) 44, 825
HindII GTYRAC 2 cut(s) 44, 825
HindIII AAGCTT 1 cut(s) 864
HinfI GANTC 5 cut(s) 267, 339, 2105, 2150, 3434
HpaII CCGG 3 cut(s) 2131, 2140, 2862
HpyCH4IV ACGT 4 cut(s) 121, 453, 1191, 1460
HpyF10VI GCNNNNNNNGC 8 cut(s) 95, 482, 872, 2134, 2351, 2505, 2621, 2858
HpyF3I CTNAG 5 cut(s) 146, 978, 1830, 2702, 3049
HpySE526I ACGT 4 cut(s) 121, 453, 1191, 1460
Ksp22I TGATCA 1 cut(s) 1206
LmnI GCTCC 6 cut(s) 985, 1022, 2133, 2188, 2900, 3381
LweI GCATC 6 cut(s) 345, 1671, 1718, 2332, 2434, 2633
MaeII ACGT 4 cut(s) 121, 453, 1191, 1460
MaeIII GTNAC 9 cut(s) 238, 652, 712, 949, 1084, 2092, 2213, 2573, 2602
MbiI CCGCTC 1 cut(s) 622
MfeI CAATTG 2 cut(s) 2277, 2358
MflI RGATCY 5 cut(s) 178, 1170, 2887, 2917, 3087
MhlI GDGCHC 3 cut(s) 688, 1324, 2958
MlsI TGGCCA 1 cut(s) 3239
MluNI TGGCCA 1 cut(s) 3239
MlyI GAGTC 3 cut(s) 261, 333, 2114
MmeI TCCRAC 6 cut(s) 94, 343, 1487, 2206, 2577, 3124
Mox20I TGGCCA 1 cut(s) 3239
MscI TGGCCA 1 cut(s) 3239
MseI TTAA 2 cut(s) 792, 1056
MslI CAYNNNNRTG 5 cut(s) 1202, 1955, 2785, 2831, 2852
Msp20I TGGCCA 1 cut(s) 3239
MspI CCGG 3 cut(s) 2131, 2140, 2862
MspR9I CCNGG 6 cut(s) 813, 1905, 2140, 2141, 2235, 2590
MunI CAATTG 2 cut(s) 2277, 2358
Mva1269I GAATGC 1 cut(s) 1477
MvaI CCWGG 4 cut(s) 813, 1905, 2235, 2590
MwoI GCNNNNNNNGC 8 cut(s) 95, 482, 872, 2134, 2351, 2505, 2621, 2858
NciI CCSGG 2 cut(s) 2140, 2141
NcoI CCATGG 3 cut(s) 1157, 3138, 3220
NdeI CATATG 2 cut(s) 690, 1077
NlaIV GGNNCC 5 cut(s) 568, 1118, 1172, 2129, 2896
NmuCI GTSAC 4 cut(s) 652, 1084, 2092, 2213
NspI RCATGY 2 cut(s) 1517, 2784
OliI CACNNNNGTG 1 cut(s) 2831
PaeI GCATGC 1 cut(s) 2784
PaeR7I CTCGAG 1 cut(s) 1857
PceI AGGCCT 2 cut(s) 700, 1903
PciI ACATGT 1 cut(s) 1513
PctI GAATGC 1 cut(s) 1477
PfeI GAWTC 2 cut(s) 2150, 3434
PleI GAGTC 3 cut(s) 261, 333, 2113
PpsI GAGTC 3 cut(s) 261, 333, 2113
Ppu21I YACGTR 1 cut(s) 1461
PpuMI RGGWCCY 1 cut(s) 566
PscI ACATGT 1 cut(s) 1513
Psp5II RGGWCCY 1 cut(s) 566
Psp6I CCWGG 4 cut(s) 811, 1903, 2233, 2588
PspFI CCCAGC 1 cut(s) 1940
PspGI CCWGG 4 cut(s) 811, 1903, 2233, 2588
PspN4I GGNNCC 5 cut(s) 568, 1118, 1172, 2129, 2896
PspPI GGNCC 5 cut(s) 116, 365, 566, 1117, 2498
PspPPI RGGWCCY 1 cut(s) 566
PstI CTGCAG 2 cut(s) 495, 2833
PsuI RGATCY 5 cut(s) 178, 1170, 2887, 2917, 3087
RseI CAYNNNNRTG 5 cut(s) 1202, 1955, 2785, 2831, 2852
SaqAI TTAA 2 cut(s) 792, 1056
Sau96I GGNCC 5 cut(s) 116, 365, 566, 1117, 2498
ScaI AGTACT 3 cut(s) 378, 1695, 2707
SchI GAGTC 3 cut(s) 261, 333, 2114
ScrFI CCNGG 6 cut(s) 813, 1905, 2140, 2141, 2235, 2590
SduI GDGCHC 3 cut(s) 688, 1324, 2958
SfaNI GCATC 6 cut(s) 345, 1671, 1718, 2332, 2434, 2633
SfcI CTRYAG 6 cut(s) 491, 1219, 2403, 2829, 3267, 3447
SfiI GGCCNNNNNGGCC 1 cut(s) 2505
Sfr274I CTCGAG 1 cut(s) 1857
SinI GGWCC 4 cut(s) 116, 365, 566, 1117
SlaI CTCGAG 1 cut(s) 1857
SmaI CCCGGG 1 cut(s) 2141
SmiMI CAYNNNNRTG 5 cut(s) 1202, 1955, 2785, 2831, 2852
SmlI CTYRAG 5 cut(s) 254, 584, 1676, 1857, 3419
SmoI CTYRAG 5 cut(s) 254, 584, 1676, 1857, 3419
SphI GCATGC 1 cut(s) 2784
SseBI AGGCCT 2 cut(s) 700, 1903
SsiI CCGC 1 cut(s) 622
SspI AATATT 3 cut(s) 235, 2313, 2750
StuI AGGCCT 2 cut(s) 700, 1903
StyD4I CCNGG 6 cut(s) 811, 1903, 2138, 2139, 2233, 2588
StyI CCWWGG 3 cut(s) 1157, 3138, 3220
TaiI ACGT 4 cut(s) 124, 456, 1194, 1463
TaqI TCGA 7 cut(s) 270, 522, 1309, 1800, 1858, 2306, 2340
TatI WGTACW 7 cut(s) 376, 597, 1369, 1693, 2705, 2733, 3045
TfiI GAWTC 2 cut(s) 2150, 3434
Tru1I TTAA 2 cut(s) 792, 1056
Tru9I TTAA 2 cut(s) 792, 1056
TscAI CASTG 9 cut(s) 78, 90, 1228, 2391, 2398, 2777, 2833, 2838, 2991
TseFI GTSAC 4 cut(s) 652, 1084, 2092, 2213
Tsp45I GTSAC 4 cut(s) 652, 1084, 2092, 2213
TspGWI ACGGA 1 cut(s) 483
TspMI CCCGGG 1 cut(s) 2139
TspRI CASTG 9 cut(s) 78, 90, 1228, 2391, 2398, 2777, 2833, 2838, 2991
VpaK11BI GGWCC 4 cut(s) 116, 365, 566, 1117
XagI CCTNNNNNAGG 1 cut(s) 647
XapI RAATTY 9 cut(s) 725, 882, 901, 1490, 1636, 2659, 2719, 2974, 3333
XceI RCATGY 2 cut(s) 1517, 2784
XcmI CCANNNNNNNNNTGG 1 cut(s) 1295
XhoI CTCGAG 1 cut(s) 1857
XmaI CCCGGG 1 cut(s) 2139
XmiI GTMKAC 2 cut(s) 912, 2968
ZrmI AGTACT 3 cut(s) 378, 1695, 2707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.