RLG00000002931
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000493
Physical Location & Seq
Reverse (-)
41873994 .. 41883306
9313 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002931

Sequence Viewer

Length: 4191 bp
ATGAACATCACATTCAGCCAAGAACAAGAAGAAGAGGAAATCATCGAGCCTCCTTCCGGTGAAGGAGCCGATGAGGAGTCCAGTCTGACAATGACGACGTACTGGTCTGCCGTAGAGCGGTTACCTACCTATTCCCGAACAAGGAGAGGAATTCTACTCGGCCAAGGTCAAGAGGAGGACGGTGAAAGCAGAGAGATTGATATCAAGAAGAATCTTGGACTACGAGAGAGGAAGACTTTGTTGGAGAGGCTGGTGCAGACTGTTGAGCAAGATAATGAAAAGTTGTTGCGCAAGATCAAGGACCGCATGAATAGAGTTGGACTTGAATTTCCACCAACTGAAGTGCGGTTCGAACATTTAAATGCTGAAGCAGAGGCTTATGTAGGAACCAGGGCGTCGTCACCTACAGTGTTCAACTTCTCCATCAATATGTTAGAGGGGTTCCTGAATTGTTTTCACATTCTTCCAAGTAAAAAGAGAGCATTTCCAATCCTTCATGATGTAAGTGGGAGCATCAAGCCAAGAAGAATGACACTTCTTTTAGGGCCACCTGGCTCTGGAAAGACCACGTTCTTATTGGCGTTGGCTGGAAAACTGGGTAAAGCTAATCTAAAATTTTCGGGGAGAGTTACATACAATGGAAGTGAGATGGATGAGTTTGTATCAGACAGGATGTCAGCTTATGTTACTCAACACGATCTACATATTCCAGAACTGACAGTGAGAGAGACACTGGCTTTTGCAGCTAGATGTCAAGGTGTTGGACCAGGTTATGAAATGTTGGTGGAATTATTAAGGAGAGAAAAGGCTGCAAATATTATGCCAGATCTTGATCTTGATCTCTACATGAAGGCAGCAGCACTAGAAGGACAGGAAGCTAATATAGTTACAGACTTTATTCTTAAGATTTTGGGACTCGAAGATTGTGCTGACATTATGGTAGGAGACGAAATGACCAGAGGTATATCTGGTGGACAGAAAAAGAGACTCACCACAGGGGAGATGCTGGTTGGACCAGAAAGAGTCCTTCTGATGGATGAAATATCCAATGGGCTGGACAGCTCAACAACATTGCAATTAGTGAAATCACTACAACAATATGTCCACATTCTGAACGGAACTGCATTAATCGCTCTACTGCAGCCAGCACCGGAGACATATGATCTCTTTGATGATATAATTCTCCTCTCGGATGGCTACATCATTTATCAGGGTCCACGTGAGAATGTTCTTGAGTTCTTTGAGTACATGGGCTTCAAATGTCCAGAGAGGAAAGGAGTTGCTGATTTCCTGCAAGAGGTGACATCAAGAAAAGATCAAGAGCAATACTGGGCTCATAGGGATAGGCCTTATAGATTTGTAACAACCAAGGCATTCTCTGAAGCAATGCAGTTATTTCACATCGGTAGAAAAGTTGGTGATGAGCTTTCTATTCCATTTAACAAGTCCGAAGGCCACCCTGCTGCATTGTCAACTAAAAAGTATGGTGTTAATAAGAAGGAATTGTTCAAAGCTTGTATGGCCAGACAAATTTTGCTTACAAAGAGGAATTCGTTTGTCTACATTTTCAAATTGGTTCAGCTTATTCTAGTTGCTCTTACAACAATGACATTATTTTTACGTACTGAGATGCACCGGAACACAGTAGCAGATGGTGCGATTTATATGGGAGCTTTATTCTATACACTACTCGCAATTATGCTTAACGGGTTTCCTGAGCTTCATATGACAGTCACTAGACTGCCTGTGTTTTTCAAGCAAAGGGACCATCTGTTCTATCCTGCTTGGGCGTACTCTTTACCCACATGGATGATAAGGATCCCTATAACTTTTGTGGACGTTTCCATCTGGGTGATCATAACTTACTACCCTATAGGTTATGATCCGAGCATTGAAAGGTTAATTTTATTTGGTCAGACAGATCAAGTTAATTGGTTATCAAATATAATTATGAAAATTTCATTAGGAGTTATGGTTTTGAAGTATCGAGGCATATTTCCGGAGGCACGCTTCTACTGGATTGGATTAGTAGCTTTGATTGGATTTATTCTTCTGTTGAATTTCCTTTTCACCTTGGCACTTCAGTATCTTGATCCACTTGAGATGCCCCAAGCAGTACTATCCAATGAGGCATCCTCCACAACAGAAGATAGCAGAGTTGCAAGCAATCCTACTAGGCAGCATGGCATGGTTCTTCCATTTGAACCCCTTTCAATTACTTTCGAGGAAATCAGATATGCTGTTGATTTGCCTAAGGAAATGAAAGATCAAAGCAAAACTGGGGATCGGCTAGAACTTCTTAAGGGCGTGAGTGGTGCTTTTAGGCCTGGAGTTCTAACAGCTTTGATGGGTGTTAGTGGCGCTGGAAAAACCACTCTCATGGATGTCTTGGCAGGAAGGAAGACAAGTGGATATATTGAGGGTAGAATCACCTTATCTGGATATGCAAAAAGGCAAGAAACATTTGCTCGCATATCAGGATACTGTGAGCAAACAGATATACACTCCCCTCATGTCACTGTTTACGAGTCTTTGGTTTATTCTGCATGGCTCCGGTTGCCCCGTGAGGTTGATTCCCCAACCAGAAAGATGTTTATTGAGGAAGTCATGGAGCTTGTAGAAATGGCCTCAATAAGTGACAGAGTTGTTGGATTTCCAGGAGTGAATGGTCTCTCAACTGAACAACGCAAAAGGCTAACAATTGCAGTAGAGCTTGTTGCCAACCCCTCAATAATATTTATGGATGAGCCGACCTCTGGCCTTGATGCTAGGGCAGCAGCAATTGTAATGAGAACAGTGAGGAATACAGTGGACACCGGGAGAACTGTAGTCTGTACCATCCACCAACCAAGCATTGATATATTTGATGCTTTTGATGAGATGTTGCTTTTGAAACGCGGGGGTGAAGAAATATATGTTGGCTCTTTAGGCCACCATTCTTCCCAGATGATCGAATACTTTGAGGAGATTTGTGGAGTTTCTAAAATAAAGGATGGTTACAATCCGGCAACTTGGATGTTGGAGGTTACTTCTCCAGCACAAGAAGCATCTCTTGGGGTTGATTTTGCATACATATACAAAAACTCAGAAATATTTAGGAAAAATAAGGCATTGATAAAAGAACTAAGTACTCCCGCCCCAAATTCAAAGGACTTGTACTTCCCAACACGATATTCAGAGTCTTTCTTCACCCAGTGTCTAGCTTGCCTATGGAAACAGCATGTATCGTATTGGCGAAACCCTCAATACACTGCGGTGAGACTTCTTTTCACAGCTACGATGGCTGCACTACTTGGATCATTTTTCTGGGATCTTGGTTCCAAAAGACAAAAGCAAAGAGATCTCTTTAGCGCGCTGGGTTCTATGTACGCTGCTGTTCTCTTTATTGGTATACAAAATTCCTTATCAGTGCAGCCAGTTGTACGTGTTGAGAGGTTGGTCTCTTACAGAGAAACGGCAGCTGGAATGCACTCGGCTTTTCCATTTGCCTTTGGACAGGCCGTCATTGAGATCCCATACACTTTGATCCAGACTATCATATATGGGGTTATAGTATACTCCATGGTTGGATTTGATTGGACGGTCAGCAAGTTCTTTTTTTATCTATTGTTCATGTACCTCACCTTCTTATACTTCACCTTCCATGGGATGATGATTGTGTCAATTACTCCGAGCAACACCATGTCTGCTGTAGTTTCGTCCGCCTTCTACCCATTATGGAATGTCATTTCAGGATTTCTCATTCCCAAAACAAGAATTCCAATATGGTGGAGATGGTTCTATTGGGTCAGCCCGACCTCTTGGAGCTTGTATGGATTGTTTTCTTCCCAGTTTGGCGGCCTCAAGGACACCCTTGATTCCGGTGAAACTCATGATAAGGACTGCAGCGAAGGTGGTCGGGATCGGCAGCGTGATGCATGGCGGGCTCTGAGGCTCGTCGACGGTTTCGTCATCCAGGAAGTCCATGCAGACAGCCTCGATCCCAGTCGCGGCGGGGATATTGGAGCACAACGTGGTCGACTACGGCGGCGAGGCGAGTTGGTGATGGAGGCTGCGAGAGCAGATGCTGAGGCTCGACTAGCAGCCTACAAGGTCTGCTGGAACACTGGCTGTTACGACTGTGACATTCTCACCTCTAATGGTGATGGCGGTGATGCTGCACGCGGTTGCAGTTTTGTTGAAGCAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1397

Amino Acids

157.57

Weight (kDa)

6.02

Isoelectric Point (pI)

42.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 165 - 348 2.2e-17 ABC transporter
ABC2_membrane_7 PF19055 381 - 464 7.7e-06 ABC-2 type transporter
ABC2_membrane PF01061 502 - 637 1.1e-22 ABC-2 type transporter
PDR_assoc PF08370 652 - 706 2.6e-21 Plant PDR ABC transporter associated
ABC_tran PF00005 767 - 919 1e-20 ABC transporter
ABC2_membrane PF01061 1064 - 1278 3e-53 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000493)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29820 FvH4_2g29820 FvH4_2g29822 FvH4_2g29824 FvH4_2g29824 FvH4_2g29824 FvH4_2g29840 FvH4_2g29840 FvH4_2g29840 FvH4_5g00550 FvH4_5g00550
malus_domestica MD02G1032700.v1.1 MD08G1140900.v1.1 MD08G1141400.v1.1 MD15G1117800.v1.1 MD15G1117900.v1.1
prunus_persica Prupe.1G471200_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471400_v2.0.a1 Prupe.7G242900_v2.0.a1
pyrus_communis pycom02g02690 pycom08g11890
rosa_chinensis RchiOBHm_Chr2g0111741 RchiOBHm_Chr2g0145991 RchiOBHm_Chr6g0298851 RchiOBHm_Chr6g0298871 RchiOBHm_Chr6g0298891 RchiOBHm_Chr6g0298901 RchiOBHm_Chr6g0298911 RchiOBHm_Chr7g0201801 RchiOBHm_Chr7g0211531 RchiOBHm_Chr7g0214601
rosa_laevigata RLG00000002931 RLG00000003000 RLG00000003647 RLG00000011476 RLG00000011479
rosa_multiflora Rmu_sc0001030.1_g000012 Rmu_sc0001548.1_g000041 Rmu_ssc0000397.1_g000033 Rmu_ssc0000397.1_g000036 Rmu_ssc0000397.1_g000037 Rmu_ssc0000397.1_g000039 Rmu_ssc0000397.1_g000048
rosa_roxburghii Rroxscaffold_1G00003830 Rroxscaffold_3G00247810 Rroxscaffold_3G00255060 Rroxscaffold_7G00169220 Rroxscaffold_7G00169230 Rroxscaffold_7G00169280
rosa_rugosa Rorug02G0223600 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283200 Rorug06G0283300 Rorug06G0283400 Rorug06G0283500 Rorug07G0066000
rosa_samantha Rh2AG511300 Rh2CG235100 Rh2CG496400 Rh2DG330900 Rh6BG403100 Rh6BG403300 Rh6BG403400 Rh6BG403500 Rh6CG408700 Rh6CG408900 Rh6CG409000 Rh6CG409200 Rh6DG395800 Rh6DG395900 Rh6DG396000 Rh6DG396200 Rh7AG193400 Rh7BG195100 Rh7BG436600 Rh7DG199300 Rh7DG265500
rosa_wichuraiana Rw2G016900 Rw6G034570 Rw6G034580 Rw6G034590 Rw6G034600 Rw7G016900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 103, 3947
Acc16I TGCGCA 1 cut(s) 290
AccBSI CCGCTC 1 cut(s) 118
AccI GTMKAC 5 cut(s) 1560, 3391, 3555, 3939, 4018
AccII CGCG 4 cut(s) 2898, 3353, 3990, 4164
AccIII TCCGGA 1 cut(s) 1999
AcoI YGGCCR 2 cut(s) 160, 1521
AcuI CTGAAG 4 cut(s) 360, 387, 1401, 2066
AcvI CACGTG 1 cut(s) 1220
AcyI GRCGYC 1 cut(s) 395
AdeI CACNNNGTG 2 cut(s) 3195, 4013
AfiI CCNNNNNNNGG 9 cut(s) 56, 117, 141, 557, 1033, 1297, 2756, 3645, 3989
AflII CTTAAG 2 cut(s) 902, 2300
AflIII ACRYGT 1 cut(s) 3424
AhdI GACNNNNNGTC 2 cut(s) 673, 3500
AjnI CCWGG 6 cut(s) 389, 550, 766, 2326, 2656, 3954
AjuI GAANNNNNNNTTGG 8 cut(s) 224, 256, 2901, 2933, 3036, 3068, 3157, 3189
AleI CACNNNNGTG 1 cut(s) 3254
AloI GAACNNNNNNTCC 2 cut(s) 1757, 1789
Alw21I GWGCWC 1 cut(s) 4009
Alw26I GTCTC 7 cut(s) 722, 939, 979, 1148, 2675, 3253, 3445
AlwNI CAGNNNCTG 1 cut(s) 4067
Aor13HI TCCGGA 1 cut(s) 1999
AseI ATTAAT 1 cut(s) 1127
AspLEI GCGC 4 cut(s) 291, 2363, 3353, 3355
AspS9I GGNCC 6 cut(s) 301, 545, 764, 1013, 1214, 1765
AsuC2I CCSGG 1 cut(s) 2818
AsuII TTCGAA 1 cut(s) 351
AvaII GGWCC 5 cut(s) 301, 764, 1013, 1214, 1765
AxyI CCTNAGG 1 cut(s) 2253
BaeI ACNNNNGTAYC 2 cut(s) 2069, 2102
BalI TGGCCA 1 cut(s) 1523
BamHI GGATCC 1 cut(s) 1818
BanII GRGCYC 2 cut(s) 1336, 3928
BarI GAAGNNNNNNTAC 4 cut(s) 1238, 1270, 3608, 3640
BbrPI CACGTG 1 cut(s) 1220
BbsI GAAGAC 2 cut(s) 239, 2408
Bbv12I GWGCWC 1 cut(s) 4009
BbvCI CCTCAGC 1 cut(s) 4068
BceAI ACGGC 4 cut(s) 95, 3471, 3485, 4040
BcgI CGANNNNNNTGC 2 cut(s) 908, 942
BciT130I CCWGG 6 cut(s) 391, 552, 768, 2328, 2658, 3956
BciVI GTATCC 1 cut(s) 2474
BclI TGATCA 1 cut(s) 1854
BcnI CCSGG 1 cut(s) 2818
BcoDI GTCTC 7 cut(s) 722, 939, 979, 1148, 2675, 3253, 3445
BfaI CTAG 9 cut(s) 747, 863, 1589, 1737, 2175, 2291, 2769, 3200, 4079
BfmI CTRYAG 6 cut(s) 405, 1139, 1872, 2826, 3690, 3883
BfoI RGCGCY 1 cut(s) 2364
BfrI CTTAAG 2 cut(s) 902, 2300
BfuI GTATCC 1 cut(s) 2474
BglII AGATCT 2 cut(s) 826, 3340
BmcAI AGTACT 2 cut(s) 2118, 3130
Bme1390I CCNGG 7 cut(s) 391, 552, 768, 2328, 2658, 2818, 3956
Bme18I GGWCC 5 cut(s) 301, 764, 1013, 1214, 1765
BmeRI GACNNNNNGTC 2 cut(s) 673, 3500
BmgT120I GGNCC 6 cut(s) 301, 545, 764, 1013, 1214, 1765
BmiI GGNNCC 8 cut(s) 67, 388, 443, 1215, 1766, 1820, 2552, 3319
BmrFI CCNGG 7 cut(s) 391, 552, 768, 2328, 2658, 2818, 3956
BmrI ACTGGG 6 cut(s) 605, 1339, 2289, 3187, 3823, 3978
BmuI ACTGGG 6 cut(s) 605, 1339, 2289, 3187, 3823, 3978
BpiI GAAGAC 2 cut(s) 239, 2408
BplI GAGNNNNNCTC 4 cut(s) 2120, 2152, 2738, 2770
BpmI CTGGAG 2 cut(s) 2349, 3018
Bpu10I CCTNAGC 2 cut(s) 1716, 4068
Bpu14I TTCGAA 1 cut(s) 351
BpuEI CTTGAG 3 cut(s) 1253, 2120, 3827
BpuMI CCSGG 1 cut(s) 2818
BsaAI YACGTR 3 cut(s) 1220, 1622, 3425
BsaBI GATNNNNATC 4 cut(s) 200, 831, 837, 1817
BsaHI GRCGYC 1 cut(s) 395
BsaI GGTCTC 2 cut(s) 2675, 3445
BsaJI CCNNGG 6 cut(s) 163, 390, 1368, 2073, 3561, 3643
BsaWI WCCGGW 6 cut(s) 56, 1150, 1635, 1999, 2553, 3860
BsaXI ACNNNNNCTCC 2 cut(s) 1959, 1989
Bsc4I CCNNNNNNNGG 9 cut(s) 56, 117, 141, 557, 1033, 1297, 2756, 3645, 3989
Bse21I CCTNAGG 1 cut(s) 2253
Bse3DI GCAATG 2 cut(s) 1070, 1392
Bse8I GATNNNNATC 4 cut(s) 200, 831, 837, 1817
BseAI TCCGGA 1 cut(s) 1999
BseBI CCWGG 6 cut(s) 391, 552, 768, 2328, 2658, 3956
BseDI CCNNGG 6 cut(s) 163, 390, 1368, 2073, 3561, 3643
BseJI GATNNNNATC 4 cut(s) 200, 831, 837, 1817
BseLI CCNNNNNNNGG 9 cut(s) 56, 117, 141, 557, 1033, 1297, 2756, 3645, 3989
BseMI GCAATG 2 cut(s) 1070, 1392
BseMII CTCAG 5 cut(s) 1617, 1707, 3099, 3920, 4059
BsePI GCGCGC 1 cut(s) 3351
BseRI GAGGAG 4 cut(s) 89, 188, 1175, 2978
BseYI CCCAGC 1 cut(s) 3355
BsgI GTGCAG 4 cut(s) 275, 3270, 3431, 4143
Bsh1236I CGCG 4 cut(s) 2898, 3353, 3990, 4164
BsiHKAI GWGCWC 1 cut(s) 4009
BsiSI CCGG 8 cut(s) 57, 1151, 1636, 2000, 2554, 2817, 3005, 3861
BslFI GGGAC 2 cut(s) 927, 1778
BslI CCNNNNNNNGG 9 cut(s) 56, 117, 141, 557, 1033, 1297, 2756, 3645, 3989
BsmAI GTCTC 7 cut(s) 722, 939, 979, 1148, 2675, 3253, 3445
BsmBI CGTCTC 1 cut(s) 939
BsmFI GGGAC 2 cut(s) 927, 1778
BsmI GAATGC 2 cut(s) 1373, 3471
Bso31I GGTCTC 2 cut(s) 2675, 3445
Bsp119I TTCGAA 1 cut(s) 351
Bsp1286I GDGCHC 3 cut(s) 1336, 3928, 4009
Bsp13I TCCGGA 1 cut(s) 1999
Bsp19I CCATGG 2 cut(s) 3561, 3643
BspCNI CTCAG 5 cut(s) 1618, 1708, 3098, 3921, 4060
BspEI TCCGGA 1 cut(s) 1999
BspFNI CGCG 4 cut(s) 2898, 3353, 3990, 4164
BspHI TCATGA 2 cut(s) 496, 3871
BspLI GGNNCC 8 cut(s) 67, 388, 443, 1215, 1766, 1820, 2552, 3319
BspMAI CTGCAG 2 cut(s) 1143, 3887
BspT104I TTCGAA 1 cut(s) 351
BspTI CTTAAG 2 cut(s) 902, 2300
BspTNI GGTCTC 2 cut(s) 2675, 3445
BsrBI CCGCTC 1 cut(s) 118
BsrDI GCAATG 2 cut(s) 1070, 1392
BssECI CCNNGG 6 cut(s) 163, 390, 1368, 2073, 3561, 3643
BssHII GCGCGC 1 cut(s) 3351
BssNAI GTATAC 2 cut(s) 3392, 3556
BssNI GRCGYC 1 cut(s) 395
BssT1I CCWWGG 5 cut(s) 163, 1368, 2073, 3561, 3643
Bst1107I GTATAC 2 cut(s) 3392, 3556
Bst2UI CCWGG 6 cut(s) 391, 552, 768, 2328, 2658, 3956
Bst6I CTCTTC 1 cut(s) 27
BstACI GRCGYC 1 cut(s) 395
BstAFI CTTAAG 2 cut(s) 902, 2300
BstAPI GCANNNNNTGC 1 cut(s) 1655
BstBAI YACGTR 3 cut(s) 1220, 1622, 3425
BstBI TTCGAA 1 cut(s) 351
BstC8I GCNNGC 9 cut(s) 1146, 2008, 2164, 2470, 3205, 3353, 3924, 4162, 4186
BstDEI CTNAG 7 cut(s) 1626, 1716, 2253, 3085, 3125, 3929, 4068
BstDSI CCRYGG 2 cut(s) 3561, 3643
BstEII GGTNACC 1 cut(s) 120
BstENI CCTNNNNNAGG 1 cut(s) 1295
BstFNI CGCG 4 cut(s) 2898, 3353, 3990, 4164
BstH2I RGCGCY 1 cut(s) 2364
BstHHI GCGC 4 cut(s) 291, 2363, 3353, 3355
BstMAI GTCTC 7 cut(s) 722, 939, 979, 1148, 2675, 3253, 3445
BstNI CCWGG 6 cut(s) 391, 552, 768, 2328, 2658, 3956
BstNSI RCATGY 1 cut(s) 3224
BstPI GGTNACC 1 cut(s) 120
BstSCI CCNGG 7 cut(s) 389, 550, 766, 2326, 2656, 2816, 3954
BstSFI CTRYAG 6 cut(s) 405, 1139, 1872, 2826, 3690, 3883
BstSNI TACGTA 1 cut(s) 1622
BstUI CGCG 4 cut(s) 2898, 3353, 3990, 4164
BstV2I GAAGAC 2 cut(s) 239, 2408
BstX2I RGATCY 5 cut(s) 826, 1818, 3310, 3340, 3510
BstXI CCANNNNNNTGG 3 cut(s) 1054, 2380, 3768
BstYI RGATCY 5 cut(s) 826, 1818, 3310, 3340, 3510
BstZ17I GTATAC 2 cut(s) 3392, 3556
Bsu36I CCTNAGG 1 cut(s) 2253
BsuI GTATCC 1 cut(s) 2474
BtgI CCRYGG 2 cut(s) 3561, 3643
BtsI GCAGTG 1 cut(s) 3249
Cac8I GCNNGC 9 cut(s) 1146, 2008, 2164, 2470, 3205, 3353, 3924, 4162, 4186
CaiI CAGNNNCTG 1 cut(s) 4067
CciI TCATGA 2 cut(s) 496, 3871
CfoI GCGC 4 cut(s) 291, 2363, 3353, 3355
Cfr13I GGNCC 6 cut(s) 301, 545, 764, 1013, 1214, 1765
CseI GACGC 1 cut(s) 384
CsiI ACCWGGT 1 cut(s) 766
DdeI CTNAG 7 cut(s) 1626, 1716, 2253, 3085, 3125, 3929, 4068
DraI TTTAAA 1 cut(s) 360
DraIII CACNNNGTG 2 cut(s) 3195, 4013
DrdI GACNNNNNNGTC 2 cut(s) 103, 3947
DriI GACNNNNNGTC 2 cut(s) 673, 3500
DseDI GACNNNNNNGTC 2 cut(s) 103, 3947
EaeI YGGCCR 2 cut(s) 160, 1521
Eam1104I CTCTTC 1 cut(s) 27
Eam1105I GACNNNNNGTC 2 cut(s) 673, 3500
EarI CTCTTC 1 cut(s) 27
EciI GGCGGA 1 cut(s) 3691
Eco105I TACGTA 1 cut(s) 1622
Eco130I CCWWGG 5 cut(s) 163, 1368, 2073, 3561, 3643
Eco147I AGGCCT 2 cut(s) 1348, 2326
Eco24I GRGCYC 2 cut(s) 1336, 3928
Eco31I GGTCTC 2 cut(s) 2675, 3445
Eco32I GATATC 1 cut(s) 202
Eco47I GGWCC 5 cut(s) 301, 764, 1013, 1214, 1765
Eco57I CTGAAG 4 cut(s) 360, 387, 1401, 2066
Eco72I CACGTG 1 cut(s) 1220
Eco81I CCTNAGG 1 cut(s) 2253
Eco91I GGTNACC 1 cut(s) 120
EcoNI CCTNNNNNAGG 1 cut(s) 1295
EcoO65I GGTNACC 1 cut(s) 120
EcoRI GAATTC 3 cut(s) 150, 1549, 3756
EcoRII CCWGG 6 cut(s) 389, 550, 766, 2326, 2656, 3954
EcoRV GATATC 1 cut(s) 202
EcoT14I CCWWGG 5 cut(s) 163, 1368, 2073, 3561, 3643
EcoT22I ATGCAT 1 cut(s) 3919
EcoT38I GRGCYC 2 cut(s) 1336, 3928
ErhI CCWWGG 5 cut(s) 163, 1368, 2073, 3561, 3643
Esp3I CGTCTC 1 cut(s) 939
FalI AAGNNNNNCTT 2 cut(s) 3036, 3068
FaqI GGGAC 2 cut(s) 927, 1778
FauI CCCGC 4 cut(s) 2891, 3142, 3915, 3986
FauNDI CATATG 2 cut(s) 1159, 1725
FbaI TGATCA 1 cut(s) 1854
FblI GTMKAC 5 cut(s) 1560, 3391, 3555, 3939, 4018
FriOI GRGCYC 2 cut(s) 1336, 3928
FspBI CTAG 9 cut(s) 747, 863, 1589, 1737, 2175, 2291, 2769, 3200, 4079
FspI TGCGCA 1 cut(s) 290
GlaI GCGC 4 cut(s) 290, 2362, 3352, 3354
GsaI CCCAGC 1 cut(s) 3359
GsuI CTGGAG 2 cut(s) 2349, 3018
HaeII RGCGCY 1 cut(s) 2364
HapII CCGG 8 cut(s) 57, 1151, 1636, 2000, 2554, 2817, 3005, 3861
HgaI GACGC 1 cut(s) 384
HhaI GCGC 4 cut(s) 291, 2363, 3353, 3355
Hin1I GRCGYC 1 cut(s) 395
Hin6I GCGC 4 cut(s) 289, 2361, 3351, 3353
HinP1I GCGC 4 cut(s) 289, 2361, 3351, 3353
HincII GTYRAC 3 cut(s) 1473, 3940, 4019
HindII GTYRAC 3 cut(s) 1473, 3940, 4019
HindIII AAGCTT 1 cut(s) 1512
HpaII CCGG 8 cut(s) 57, 1151, 1636, 2000, 2554, 2817, 3005, 3861
Hpy99I CGWCG 4 cut(s) 100, 400, 3941, 3944
HpyCH4IV ACGT 7 cut(s) 98, 569, 1219, 1621, 1839, 3424, 4012
HpyF3I CTNAG 7 cut(s) 1626, 1716, 2253, 3085, 3125, 3929, 4068
HpySE526I ACGT 7 cut(s) 98, 569, 1219, 1621, 1839, 3424, 4012
Hsp92I GRCGYC 1 cut(s) 395
HspAI GCGC 4 cut(s) 289, 2361, 3351, 3353
Kpn2I TCCGGA 1 cut(s) 1999
Ksp22I TGATCA 1 cut(s) 1854
LmnI GCTCC 7 cut(s) 65, 510, 1670, 2556, 2611, 3804, 4004
MabI ACCWGGT 1 cut(s) 766
MaeI CTAG 9 cut(s) 747, 863, 1589, 1737, 2175, 2291, 2769, 3200, 4079
MaeII ACGT 7 cut(s) 98, 569, 1219, 1621, 1839, 3424, 4012
MbiI CCGCTC 1 cut(s) 118
MfeI CAATTG 2 cut(s) 2700, 2781
MflI RGATCY 5 cut(s) 826, 1818, 3310, 3340, 3510
MhlI GDGCHC 3 cut(s) 1336, 3928, 4009
MlsI TGGCCA 1 cut(s) 1523
MluNI TGGCCA 1 cut(s) 1523
MlyI GAGTC 6 cut(s) 86, 909, 981, 1032, 2537, 3188
MmeI TCCRAC 7 cut(s) 222, 298, 742, 991, 2629, 3000, 3547
Mox20I TGGCCA 1 cut(s) 1523
Mph1103I ATGCAT 1 cut(s) 3919
MroI TCCGGA 1 cut(s) 1999
MscI TGGCCA 1 cut(s) 1523
MslI CAYNNNNRTG 6 cut(s) 360, 428, 1808, 1850, 2378, 3254
Msp20I TGGCCA 1 cut(s) 1523
MspA1I CMGCKG 1 cut(s) 3461
MspCI CTTAAG 2 cut(s) 902, 2300
MspI CCGG 8 cut(s) 57, 1151, 1636, 2000, 2554, 2817, 3005, 3861
MspR9I CCNGG 7 cut(s) 391, 552, 768, 2328, 2658, 2818, 3956
MunI CAATTG 2 cut(s) 2700, 2781
Mva1269I GAATGC 2 cut(s) 1373, 3471
MvaI CCWGG 6 cut(s) 391, 552, 768, 2328, 2658, 3956
MvnI CGCG 4 cut(s) 2898, 3353, 3990, 4164
NciI CCSGG 1 cut(s) 2818
NcoI CCATGG 2 cut(s) 3561, 3643
NdeI CATATG 2 cut(s) 1159, 1725
NlaIV GGNNCC 8 cut(s) 67, 388, 443, 1215, 1766, 1820, 2552, 3319
NmeAIII GCCGAG 2 cut(s) 138, 3452
NmuCI GTSAC 6 cut(s) 399, 1300, 1732, 2515, 2636, 4121
NsbI TGCGCA 1 cut(s) 290
NsiI ATGCAT 1 cut(s) 3919
NspI RCATGY 1 cut(s) 3224
NspV TTCGAA 1 cut(s) 351
OliI CACNNNNGTG 1 cut(s) 3254
PagI TCATGA 2 cut(s) 496, 3871
PauI GCGCGC 1 cut(s) 3351
PceI AGGCCT 2 cut(s) 1348, 2326
PcsI WCGNNNNNNNCGW 1 cut(s) 3945
PctI GAATGC 2 cut(s) 1373, 3471
PfeI GAWTC 4 cut(s) 211, 2427, 2573, 3857
PfoI TCCNGGA 2 cut(s) 2656, 3954
PleI GAGTC 6 cut(s) 85, 909, 981, 1031, 2536, 3187
PmaCI CACGTG 1 cut(s) 1220
PmlI CACGTG 1 cut(s) 1220
PpsI GAGTC 6 cut(s) 85, 909, 981, 1031, 2536, 3187
Ppu21I YACGTR 3 cut(s) 1220, 1622, 3425
PshBI ATTAAT 1 cut(s) 1127
Psp6I CCWGG 6 cut(s) 389, 550, 766, 2326, 2656, 3954
PspCI CACGTG 1 cut(s) 1220
PspEI GGTNACC 1 cut(s) 120
PspFI CCCAGC 1 cut(s) 3355
PspGI CCWGG 6 cut(s) 389, 550, 766, 2326, 2656, 3954
PspN4I GGNNCC 8 cut(s) 67, 388, 443, 1215, 1766, 1820, 2552, 3319
PspPI GGNCC 6 cut(s) 301, 545, 764, 1013, 1214, 1765
PstI CTGCAG 2 cut(s) 1143, 3887
PstNI CAGNNNCTG 1 cut(s) 4067
PsuI RGATCY 5 cut(s) 826, 1818, 3310, 3340, 3510
PteI GCGCGC 1 cut(s) 3351
PvuII CAGCTG 1 cut(s) 3461
RseI CAYNNNNRTG 6 cut(s) 360, 428, 1808, 1850, 2378, 3254
SalI GTCGAC 2 cut(s) 3938, 4017
Sau96I GGNCC 6 cut(s) 301, 545, 764, 1013, 1214, 1765
ScaI AGTACT 2 cut(s) 2118, 3130
SchI GAGTC 6 cut(s) 86, 909, 981, 1032, 2537, 3188
ScrFI CCNGG 7 cut(s) 391, 552, 768, 2328, 2658, 2818, 3956
SduI GDGCHC 3 cut(s) 1336, 3928, 4009
SexAI ACCWGGT 1 cut(s) 766
SfcI CTRYAG 6 cut(s) 405, 1139, 1872, 2826, 3690, 3883
SfuI TTCGAA 1 cut(s) 351
SgrDI CGTCGACG 1 cut(s) 3938
SinI GGWCC 5 cut(s) 301, 764, 1013, 1214, 1765
SmiI ATTTAAAT 1 cut(s) 360
SmiMI CAYNNNNRTG 6 cut(s) 360, 428, 1808, 1850, 2378, 3254
SmlI CTYRAG 5 cut(s) 902, 1232, 2099, 2300, 3842
SmoI CTYRAG 5 cut(s) 902, 1232, 2099, 2300, 3842
SnaBI TACGTA 1 cut(s) 1622
SseBI AGGCCT 2 cut(s) 1348, 2326
SspI AATATT 3 cut(s) 817, 2736, 3093
SspMI CTAG 9 cut(s) 747, 863, 1589, 1737, 2175, 2291, 2769, 3200, 4079
StuI AGGCCT 2 cut(s) 1348, 2326
StyD4I CCNGG 7 cut(s) 389, 550, 766, 2326, 2656, 2816, 3954
StyI CCWWGG 5 cut(s) 163, 1368, 2073, 3561, 3643
SwaI ATTTAAAT 1 cut(s) 360
TaiI ACGT 7 cut(s) 101, 572, 1222, 1624, 1842, 3427, 4015
TatI WGTACW 4 cut(s) 1245, 2116, 3128, 3156
TauI GCSGC 3 cut(s) 3840, 3993, 4030
TfiI GAWTC 4 cut(s) 211, 2427, 2573, 3857
TseFI GTSAC 6 cut(s) 399, 1300, 1732, 2515, 2636, 4121
Tsp45I GTSAC 6 cut(s) 399, 1300, 1732, 2515, 2636, 4121
TspGWI ACGGA 1 cut(s) 1131
Vha464I CTTAAG 2 cut(s) 902, 2300
VpaK11BI GGWCC 5 cut(s) 301, 764, 1013, 1214, 1765
VspI ATTAAT 1 cut(s) 1127
XagI CCTNNNNNAGG 1 cut(s) 1295
XceI RCATGY 1 cut(s) 3224
XcmI CCANNNNNNNNNTGG 1 cut(s) 574
XmiI GTMKAC 5 cut(s) 1560, 3391, 3555, 3939, 4018
XspI CTAG 9 cut(s) 747, 863, 1589, 1737, 2175, 2291, 2769, 3200, 4079
ZrmI AGTACT 2 cut(s) 2118, 3130
Zsp2I ATGCAT 1 cut(s) 3919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.