Rw7G016900
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000493
Physical Location & Seq
Reverse (-)
17455412 .. 17464029
8618 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G016900.1

Sequence Viewer

Length: 4230 bp
ATGAACGTCATGATCAGCCAAGAACAAGAAGAAGAGCCAGTCTCCGAGCCTCCTTCCGGTGAAAAGGCCGATAAGGAGTCGAGTCAGACAATGACGACATGGGGTGCCATTGAGAGACTGCCTACGTATTCACGCACAAGGAGAGGCATTCTACTCGGGCAAGGTCAAGAGGAGGACGGTGAAAGCAGAGAGATTGATGTAAAGAAGAATCTTGGACTACCAGAGAGGAAGACTTTGTTGGAGAGGCTAGTGAAGATTGTTGAGCAAGATAATGACAAGTTCTTGCTCAAGATCAAAGACCGCATGAATAGAGTTGGACTTGAATTTCCAACGACTGAAGTGCGGTTCGAGCATTTAAATGTTGAAGCAGAAGCTTATGTAGGAAGCAGGGCATCATCTACAATGTTCAACTTCACTATTAATATGTTAGAGGGGTTCCTGAACTATTTTCACATTCTTCCAAGTAGAAAGGGACCATTTACAATCCTCCATGATGTAAGTGGGATTATCAAGCCAAGAAGAATGACACTTCTTTTAGGGCCCCCTGGCTCTGGAAAGACGACGTTACTATTGGCCTTGGCCGGAAAAGTTGGTAAAGCCAATCTAAAATTTTCGGGGAGAGTTACATACAATGGAATTGAGATGGATGAGTTTGTATCAGAGAGGACATCAGCCTATGTTAGTCAACATGATCTCCACATACCAGAATTGACAGTGAGAGAAACACTGGCTTTTGCAGCTAGATGTCAAGGTGTTGGACCACGTTATGAAATGTTGGTGGAATTACTAAGGAGAGAAAAGGCTGCAAATATTATGCCAGATCTTGATCTTGATCTCTACATGAAGGCAGCAACAGTGGAAGGACAGGAAACTAATATTGTTACAGACTTTATTCTCAAGGTTTTGGGACTCGAAGATTGTGCTGAAATTATGGTAGGAGATGAAATGACCAGAGGTATCTCTGGTGGACAAAAAAAGCGACTCACCACAGGGGAAATGCTGGTTGGACCAGAAAGAGTACTTCTCATGGATGAGATATCAAACGGGCTGGACAGTTCAACAACATTGCAAATAGTGAAATCGCTACAACAATACGTCCACATCCTCAACGGAACTGCACTAATCGCTCTACTGCAGCCAGCACCAGAGACTTATGATCTCTTCGATGATATAATTCTCCTTTCGGATGGCTACATTGTGTATCAAGGTCCCCGTGAGAATGTTCTTGAGTTCTTTGAGTACATGGGCTTCAAATGTCCAGAGCGGAAAGGAGTTGCTGATTTCCTACAAGAGGTGACATCAAGAAATGATCAAGAGCAATACTGGGCTCATATGGATAGGCCTTATAGCTTTGTAACAACCAAAGAATTTTCTGAAGCAATGCAGTTATTTCACATTGGTAGAAAACTTGGTGATGAGCTTTCTATTCCATTTAACAAGTCAGAAGGCTGCCCTGGTGCTTTGTCAACTAAAAAATATGGTGTTAGTAAGAAGGAATTGTTCAAAGCTTGTATGGCTAGACAAATTTTGCTTACAAAGAGGAATTCATTTGTCTACATTTTCAAATTGGTGCAGCTTATTCTAGTTGCTGTTACAACAATGACACTATTTTTTCATACTGAGATGGAGCGAAACACAGTTGCAGATGGTAGGATTTATATGGGAGCTTTATTCTATACGCTACTCGCAATTATGCTTAATGGGTTTGCAGAGCTTCATATGACAGTCACTAGACTGCCTGTGTTTTTCAAGCAAAGGGACCATCTGTTCTATCCTGCTTGGGCATACTCTTTACCCCCATGGCTGATAAGGATCCCTATAACTTTTGTGGACGTTTTCATCTGGGTGATCATTACTTACTACAGTGTAGGTTATGATCCGAGCGTTGAAAGGTTTTTTAAACAGTTCCTTCTACTGCTGTGTATTAGCCAGATGGCAAATGGACTGTTTCGATTGATAGGGGCACTAGGAAGGAACATAAGTATTGCAAATACATTTGGATTTGGTACTGTACTTGTCATTCTTGGTCTGTGTGGCTTTATCATATCAAAAGAGGATTTGAAGAAATGGTTGTTGTGGGGATATTGGCTCTCACCAATCACGTATGGACTGAATGCCATTGCTGTCAATGAATTTCTCGGAAAAAGTTGGAGACATGTTCCTGCCAACTCAACAGAAGCATTAGGAGTTATGGTCTTGAAGTATCAAGGCATATTCCCAGAGGCCCGCTTCTACTGGATTGGATTAGTAGCTTTGATTGGATTTATTCTTCTGTTGAATTTCCTTTTCACCTTGGCACTTCAGTATCTTGATCCACTTGAGATGCCCCAAGCAGTACTATCCAATGAGGCATCCTCTACAACAGAGGATAGCAGAGTTGCAAGCAATCCTACTATGCAGCATGGCATGGTTCTTCCATTTGAACCCCTTTCAATTACTTTCGAGGAAATCAGATATGCTGTTGATATGCCTGAGGAAATGAAAGATCAAAGCGCAACTCGAGATCGGCTAGAACTTCTGAAGGGTGTGAGTGGTACTTTTAGGCCTGGAGTTCTAACAGCTTTGATGGGTGTTAGTGGTGCTGGGAAAACCACTCTCATGGATGTCTTGGCAGGAAGGAAGACAGGTGGATATATTGAGGGGAGGATCACCTTATCTGGCTATGCAAAAAGGCAAGAAACATTTGCTCGCATATCAGGATACTGTGAGCAAACAGATATACACTCCCCTCATGTCACAGTTTACGAGTCTTTGGTTTACTCTGCATGGCTCCGATTGCCCCGGGAGGTTGATTCCCCAACCAGAAAGATGTTTATTGAGGAAGTCATGGAGCTTGTAGAATTGACCTCAATAAGTGACAGACTCGTTGGATTGCCAGGAGTGAATGGTCTCTCAACTGAACAACGCAAAAGGATAACAATTGCAGTAGAGCTTGTTGCCAACTCCTCGATAATATTTATGGATGAGCCGACTTCTGGCCTCGATGCTAGGGCAGCAGCAATTGTAATGAGAACGGTGAGGAATACAGTGGACACTGGGAGAACTGTAGTCTGTACCATCCACCAACCAAGCATTGATATATTTGATGCTTTTGATGAGATGTTGCTTCTGAAACTGGGAGGCGAAGAAATATATGTTGGCCCTTTAGGCCACCATTCTTCCCAGATGATTGAATACTTTGAGGAGATTAGTGGAGTTTCTAAAATCAAGGATGGTTACAATCCAGCAACCTGGATGTTGGAGGTTACTTCTCCAGCACAAGAAGCATCTCTTGGGGTTGATTTTGCCAACATATACAAGAATTCAGAAATATATAAGACAAATAAGGCATTGATAAAGGAACTAAGTACTCCTGCACCAAATTCAAAGGACTTGTACTTCCCAACACAATATTCGCAGTCCTTCTTTACCCAGTGTCTAGCTTGCCTATGGAAACAGAGTGTATCATATTGGCGAAACTCTCAATACACTGCAGTGAGACTTCTTTTCACAGCTATGATGGCCGGAGTACTTGGGTCATTTTTTTGGGATCTTGGCTCCAAAAGACAAAAGCAAAGAGATCTCTTTTGTGCAATGGGTTCTATGTATGCTGCTGTGCTCTTTATTGGTATACAAAATTCCTTGTCAGTGCAGCCAATTGTAGGTGTTGAGAGGTTGGTCTCTTACAGAGAAACGGCTGCTGGAATGTACTCAGCTTTTCCATTTGCCTTTGGACAGGCTATCATTGAGATCCCATACACTTTGATCCAGACTATTATATTTGGGGTTATAGCATACTCCATGGTCGGATTTGATTGGACAGTCAGCAAGTTCTTTTGTTATCTCTTGTTCATGTACCTCACCTTCTTATACTTCACCTTCCATGGCATGATGATTGTGGCCATTACTCCCAACAACACCATCTCTGCTGTAGTTTCGTCTGCCTTCTATCCATTATGGAATGTTATTTCAGGATATCTCATTCCCAAAACAAGAATTCCAATATGGTGGAGATGGTTCTATTGGGTCAGCCCAACCTCTTGGAGCTTGTATGGATTGTTTTCTTCACAGTTTGGAGGTCTCGAGGACACACTTGATTCTGGTGAAACTGTAGATCATTTTATGAGGACATATTTTGGATATAGGAAAGACTTTCTAGGTGTTGTTGTGGCTGTGCTTGTTGGGTTTTCAGCGTTCTTTGTTTTCATCTTTGCATTTGCAATCAAGAAACTGAACTTCCAAAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1409

Amino Acids

159.22

Weight (kDa)

5.99

Isoelectric Point (pI)

39.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 88 - 138 8.5e-06 ABC-transporter N-terminal
ABC_tran PF00005 163 - 346 1.8e-17 ABC transporter
ABC2_membrane PF01061 500 - 712 4.8e-48 ABC-2 type transporter
PDR_assoc PF08370 717 - 779 6.9e-23 Plant PDR ABC transporter associated
ABC_tran PF00005 840 - 992 8.4e-21 ABC transporter
ABC2_membrane PF01061 1137 - 1351 6.3e-53 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000493)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29820 FvH4_2g29820 FvH4_2g29822 FvH4_2g29824 FvH4_2g29824 FvH4_2g29824 FvH4_2g29840 FvH4_2g29840 FvH4_2g29840 FvH4_5g00550 FvH4_5g00550
malus_domestica MD02G1032700.v1.1 MD08G1140900.v1.1 MD08G1141400.v1.1 MD15G1117800.v1.1 MD15G1117900.v1.1
prunus_persica Prupe.1G471200_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471400_v2.0.a1 Prupe.7G242900_v2.0.a1
pyrus_communis pycom02g02690 pycom08g11890
rosa_chinensis RchiOBHm_Chr2g0111741 RchiOBHm_Chr2g0145991 RchiOBHm_Chr6g0298851 RchiOBHm_Chr6g0298871 RchiOBHm_Chr6g0298891 RchiOBHm_Chr6g0298901 RchiOBHm_Chr6g0298911 RchiOBHm_Chr7g0201801 RchiOBHm_Chr7g0211531 RchiOBHm_Chr7g0214601
rosa_laevigata RLG00000002931 RLG00000003000 RLG00000003647 RLG00000011476 RLG00000011479
rosa_multiflora Rmu_sc0001030.1_g000012 Rmu_sc0001548.1_g000041 Rmu_ssc0000397.1_g000033 Rmu_ssc0000397.1_g000036 Rmu_ssc0000397.1_g000037 Rmu_ssc0000397.1_g000039 Rmu_ssc0000397.1_g000048
rosa_roxburghii Rroxscaffold_1G00003830 Rroxscaffold_3G00247810 Rroxscaffold_3G00255060 Rroxscaffold_7G00169220 Rroxscaffold_7G00169230 Rroxscaffold_7G00169280
rosa_rugosa Rorug02G0223600 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283200 Rorug06G0283300 Rorug06G0283400 Rorug06G0283500 Rorug07G0066000
rosa_samantha Rh2AG511300 Rh2CG235100 Rh2CG496400 Rh2DG330900 Rh6BG403100 Rh6BG403300 Rh6BG403400 Rh6BG403500 Rh6CG408700 Rh6CG408900 Rh6CG409000 Rh6CG409200 Rh6DG395800 Rh6DG395900 Rh6DG396000 Rh6DG396200 Rh7AG193400 Rh7BG195100 Rh7BG436600 Rh7DG199300 Rh7DG265500
rosa_wichuraiana Rw2G016900 Rw6G034570 Rw6G034580 Rw6G034590 Rw6G034600 Rw7G016900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 104
AccBSI CCGCTC 1 cut(s) 1264
AccI GTMKAC 2 cut(s) 1554, 3610
AciI CCGC 4 cut(s) 301, 343, 1264, 2227
AclWI GGATC 8 cut(s) 1807, 1820, 1871, 2306, 2654, 3537, 3724, 3739
AcoI YGGCCR 3 cut(s) 579, 3501, 3879
AcuI CTGAAG 4 cut(s) 357, 1395, 2285, 2540
AfiI CCNNNNNNNGG 5 cut(s) 56, 551, 1291, 2975, 3641
AflIII ACRYGT 1 cut(s) 2155
AjnI CCWGG 5 cut(s) 544, 1453, 2545, 2875, 3230
AleI CACNNNNGTG 1 cut(s) 3473
AloI GAACNNNNNNTCC 2 cut(s) 1751, 1783
Alw21I GWGCWC 1 cut(s) 3600
Alw26I GTCTC 8 cut(s) 46, 109, 1142, 2146, 2894, 3472, 3664, 4064
AlwI GGATC 8 cut(s) 1807, 1820, 1871, 2306, 2654, 3537, 3724, 3739
Ama87I CYCGRG 4 cut(s) 155, 2499, 2781, 4061
ApaI GGGCCC 1 cut(s) 543
AseI ATTAAT 1 cut(s) 420
AspLEI GCGC 1 cut(s) 2495
AspS9I GGNCC 9 cut(s) 473, 539, 540, 758, 1007, 1208, 1759, 2224, 3140
AsuC2I CCSGG 2 cut(s) 2782, 2783
AvaI CYCGRG 4 cut(s) 155, 2499, 2781, 4061
AvaII GGWCC 5 cut(s) 473, 758, 1007, 1208, 1759
AxyI CCTNAGG 1 cut(s) 2472
BaeGI GKGCMC 2 cut(s) 543, 1966
BaeI ACNNNNGTAYC 2 cut(s) 2288, 2321
BalI TGGCCA 1 cut(s) 3881
BamHI GGATCC 1 cut(s) 1812
BanI GGYRCC 1 cut(s) 104
BanII GRGCYC 2 cut(s) 543, 1330
BarI GAAGNNNNNNTAC 4 cut(s) 1232, 1264, 3827, 3859
BbsI GAAGAC 2 cut(s) 236, 2627
Bbv12I GWGCWC 1 cut(s) 3600
BceAI ACGGC 1 cut(s) 3690
BcgI CGANNNNNNTGC 6 cut(s) 136, 170, 322, 356, 902, 936
BciT130I CCWGG 5 cut(s) 546, 1455, 2547, 2877, 3232
BciVI GTATCC 1 cut(s) 2693
BclI TGATCA 3 cut(s) 12, 1309, 1848
BcnI CCSGG 2 cut(s) 2782, 2783
BcoDI GTCTC 8 cut(s) 46, 109, 1142, 2146, 2894, 3472, 3664, 4064
BfmI CTRYAG 6 cut(s) 1133, 1861, 3045, 3471, 3909, 4089
BfuI GTATCC 1 cut(s) 2693
BglI GCCNNNNNGGC 1 cut(s) 3147
BglII AGATCT 2 cut(s) 820, 3559
BmcAI AGTACT 4 cut(s) 1020, 2337, 3349, 3510
Bme1390I CCNGG 7 cut(s) 546, 1455, 2547, 2782, 2783, 2877, 3232
Bme18I GGWCC 5 cut(s) 473, 758, 1007, 1208, 1759
BmeT110I CYCGRG 4 cut(s) 155, 2499, 2781, 4061
BmgT120I GGNCC 9 cut(s) 473, 539, 540, 758, 1007, 1208, 1759, 2224, 3140
BmrFI CCNGG 7 cut(s) 546, 1455, 2547, 2782, 2783, 2877, 3232
BmrI ACTGGG 4 cut(s) 1333, 3045, 3125, 3406
BmsI GCATC 6 cut(s) 401, 2313, 2360, 2974, 3076, 3275
BmuI ACTGGG 4 cut(s) 1333, 3045, 3125, 3406
BpiI GAAGAC 2 cut(s) 236, 2627
BplI GAGNNNNNCTC 6 cut(s) 26, 58, 1008, 1040, 2339, 2371
BpmI CTGGAG 2 cut(s) 2568, 3237
BpuEI CTTGAG 4 cut(s) 272, 881, 1247, 2339
BpuMI CCSGG 2 cut(s) 2782, 2783
BsaAI YACGTR 2 cut(s) 126, 2103
BsaBI GATNNNNATC 3 cut(s) 825, 831, 1811
BsaI GGTCTC 3 cut(s) 2894, 3664, 4064
BsaJI CCNNGG 9 cut(s) 544, 576, 1453, 1799, 2292, 2780, 2781, 3780, 3862
BsaWI WCCGGW 1 cut(s) 56
BsaXI ACNNNNNCTCC 4 cut(s) 678, 708, 2178, 2208
Bsc4I CCNNNNNNNGG 5 cut(s) 56, 551, 1291, 2975, 3641
Bse1I ACTGG 7 cut(s) 38, 732, 1328, 2240, 3040, 3120, 3412
Bse21I CCTNAGG 1 cut(s) 2472
Bse3DI GCAATG 4 cut(s) 1064, 1386, 2118, 3579
Bse8I GATNNNNATC 3 cut(s) 825, 831, 1811
BseBI CCWGG 5 cut(s) 546, 1455, 2547, 2877, 3232
BseDI CCNNGG 9 cut(s) 544, 576, 1453, 1799, 2292, 2780, 2781, 3780, 3862
BseJI GATNNNNATC 3 cut(s) 825, 831, 1811
BseLI CCNNNNNNNGG 5 cut(s) 56, 551, 1291, 2975, 3641
BseMI GCAATG 4 cut(s) 1064, 1386, 2118, 3579
BseMII CTCAG 3 cut(s) 1611, 2463, 3705
BseNI ACTGG 7 cut(s) 38, 732, 1328, 2240, 3040, 3120, 3412
BseRI GAGGAG 3 cut(s) 185, 2935, 3197
BseSI GKGCMC 2 cut(s) 543, 1966
BseYI CCCAGC 1 cut(s) 2582
BsgI GTGCAG 4 cut(s) 1101, 1592, 3339, 3650
BshNI GGYRCC 1 cut(s) 104
BsiHKAI GWGCWC 1 cut(s) 3600
BsiHKCI CYCGRG 4 cut(s) 155, 2499, 2781, 4061
BsiSI CCGG 4 cut(s) 57, 582, 2782, 3504
BslFI GGGAC 4 cut(s) 486, 921, 1194, 1772
BslI CCNNNNNNNGG 5 cut(s) 56, 551, 1291, 2975, 3641
BsmAI GTCTC 8 cut(s) 46, 109, 1142, 2146, 2894, 3472, 3664, 4064
BsmFI GGGAC 4 cut(s) 486, 921, 1194, 1772
BsmI GAATGC 2 cut(s) 147, 2119
Bso31I GGTCTC 3 cut(s) 2894, 3664, 4064
BsoBI CYCGRG 4 cut(s) 155, 2499, 2781, 4061
Bsp120I GGGCCC 1 cut(s) 539
Bsp1286I GDGCHC 4 cut(s) 543, 1330, 1966, 3600
Bsp19I CCATGG 3 cut(s) 1799, 3780, 3862
BspACI CCGC 4 cut(s) 301, 343, 1264, 2227
BspCNI CTCAG 3 cut(s) 1612, 2464, 3704
BspHI TCATGA 1 cut(s) 9
BspMAI CTGCAG 2 cut(s) 1137, 3475
BspPI GGATC 8 cut(s) 1807, 1820, 1871, 2306, 2654, 3537, 3724, 3739
BspQI GCTCTTC 1 cut(s) 27
BspT107I GGYRCC 1 cut(s) 104
BspTNI GGTCTC 3 cut(s) 2894, 3664, 4064
BsrBI CCGCTC 1 cut(s) 1264
BsrDI GCAATG 4 cut(s) 1064, 1386, 2118, 3579
BsrI ACTGG 7 cut(s) 38, 732, 1328, 2240, 3040, 3120, 3412
BssECI CCNNGG 9 cut(s) 544, 576, 1453, 1799, 2292, 2780, 2781, 3780, 3862
BssNAI GTATAC 1 cut(s) 3611
BssT1I CCWWGG 5 cut(s) 576, 1799, 2292, 3780, 3862
Bst1107I GTATAC 1 cut(s) 3611
Bst2UI CCWGG 5 cut(s) 546, 1455, 2547, 2877, 3232
Bst6I CTCTTC 2 cut(s) 27, 1166
BstBAI YACGTR 2 cut(s) 126, 2103
BstC8I GCNNGC 5 cut(s) 1140, 2227, 2383, 2689, 3424
BstDEI CTNAG 5 cut(s) 788, 1620, 2472, 3344, 3691
BstDSI CCRYGG 3 cut(s) 1799, 3780, 3862
BstENI CCTNNNNNAGG 1 cut(s) 1289
BstHHI GCGC 1 cut(s) 2495
BstMAI GTCTC 8 cut(s) 46, 109, 1142, 2146, 2894, 3472, 3664, 4064
BstMWI GCNNNNNNNGC 9 cut(s) 349, 737, 1124, 1514, 2776, 2993, 3147, 3263, 3500
BstNI CCWGG 5 cut(s) 546, 1455, 2547, 2877, 3232
BstNSI RCATGY 1 cut(s) 2159
BstSCI CCNGG 7 cut(s) 544, 1453, 2545, 2780, 2781, 2875, 3230
BstSFI CTRYAG 6 cut(s) 1133, 1861, 3045, 3471, 3909, 4089
BstSLI GKGCMC 2 cut(s) 543, 1966
BstSNI TACGTA 1 cut(s) 126
BstV2I GAAGAC 2 cut(s) 236, 2627
BstX2I RGATCY 5 cut(s) 820, 1812, 3529, 3559, 3729
BstXI CCANNNNNNTGG 4 cut(s) 2599, 3231, 3987, 4020
BstYI RGATCY 5 cut(s) 820, 1812, 3529, 3559, 3729
BstZ17I GTATAC 1 cut(s) 3611
Bsu36I CCTNAGG 1 cut(s) 2472
BsuI GTATCC 1 cut(s) 2693
BtgI CCRYGG 3 cut(s) 1799, 3780, 3862
BtsI GCAGTG 2 cut(s) 3468, 3480
Cac8I GCNNGC 5 cut(s) 1140, 2227, 2383, 2689, 3424
CciI TCATGA 1 cut(s) 9
CfoI GCGC 1 cut(s) 2495
Cfr13I GGNCC 9 cut(s) 473, 539, 540, 758, 1007, 1208, 1759, 2224, 3140
Cfr9I CCCGGG 1 cut(s) 2781
DdeI CTNAG 5 cut(s) 788, 1620, 2472, 3344, 3691
DraI TTTAAA 2 cut(s) 357, 1900
EaeI YGGCCR 3 cut(s) 579, 3501, 3879
Eam1104I CTCTTC 2 cut(s) 27, 1166
EarI CTCTTC 2 cut(s) 27, 1166
Eco105I TACGTA 1 cut(s) 126
Eco130I CCWWGG 5 cut(s) 576, 1799, 2292, 3780, 3862
Eco147I AGGCCT 2 cut(s) 1342, 2545
Eco24I GRGCYC 2 cut(s) 543, 1330
Eco31I GGTCTC 3 cut(s) 2894, 3664, 4064
Eco32I GATATC 2 cut(s) 1038, 3956
Eco47I GGWCC 5 cut(s) 473, 758, 1007, 1208, 1759
Eco57I CTGAAG 4 cut(s) 357, 1395, 2285, 2540
Eco81I CCTNAGG 1 cut(s) 2472
Eco88I CYCGRG 4 cut(s) 155, 2499, 2781, 4061
EcoNI CCTNNNNNAGG 1 cut(s) 1289
EcoO109I RGGNCCY 3 cut(s) 539, 540, 1208
EcoRI GAATTC 3 cut(s) 1543, 3301, 3975
EcoRII CCWGG 5 cut(s) 544, 1453, 2545, 2875, 3230
EcoRV GATATC 2 cut(s) 1038, 3956
EcoT14I CCWWGG 5 cut(s) 576, 1799, 2292, 3780, 3862
EcoT38I GRGCYC 2 cut(s) 543, 1330
ErhI CCWWGG 5 cut(s) 576, 1799, 2292, 3780, 3862
FalI AAGNNNNNCTT 2 cut(s) 3255, 3287
FaqI GGGAC 4 cut(s) 486, 921, 1194, 1772
FauI CCCGC 1 cut(s) 2234
FauNDI CATATG 2 cut(s) 1332, 1719
FbaI TGATCA 3 cut(s) 12, 1309, 1848
FblI GTMKAC 2 cut(s) 1554, 3610
FriOI GRGCYC 2 cut(s) 543, 1330
GlaI GCGC 1 cut(s) 2494
GsaI CCCAGC 1 cut(s) 2586
GsuI CTGGAG 2 cut(s) 2568, 3237
HapII CCGG 4 cut(s) 57, 582, 2782, 3504
HhaI GCGC 1 cut(s) 2495
Hin6I GCGC 1 cut(s) 2493
HinP1I GCGC 1 cut(s) 2493
HincII GTYRAC 2 cut(s) 686, 1467
HindII GTYRAC 2 cut(s) 686, 1467
HindIII AAGCTT 2 cut(s) 372, 1506
HinfI GANTC 9 cut(s) 77, 82, 208, 909, 981, 2747, 2792, 2862, 4076
HpaII CCGG 4 cut(s) 57, 582, 2782, 3504
Hpy99I CGWCG 1 cut(s) 565
HpyCH4IV ACGT 7 cut(s) 6, 125, 563, 763, 1095, 1833, 2102
HpyF10VI GCNNNNNNNGC 9 cut(s) 349, 737, 1124, 1514, 2776, 2993, 3147, 3263, 3500
HpyF3I CTNAG 5 cut(s) 788, 1620, 2472, 3344, 3691
HpySE526I ACGT 7 cut(s) 6, 125, 563, 763, 1095, 1833, 2102
HspAI GCGC 1 cut(s) 2493
Ksp22I TGATCA 3 cut(s) 12, 1309, 1848
LguI GCTCTTC 1 cut(s) 27
LmnI GCTCC 6 cut(s) 1627, 1664, 2775, 2830, 3542, 4023
LweI GCATC 6 cut(s) 401, 2313, 2360, 2974, 3076, 3275
MaeII ACGT 7 cut(s) 6, 125, 563, 763, 1095, 1833, 2102
MbiI CCGCTC 1 cut(s) 1264
MfeI CAATTG 3 cut(s) 2919, 3000, 3636
MflI RGATCY 5 cut(s) 820, 1812, 3529, 3559, 3729
MhlI GDGCHC 4 cut(s) 543, 1330, 1966, 3600
MlsI TGGCCA 1 cut(s) 3881
MluNI TGGCCA 1 cut(s) 3881
MlyI GAGTC 6 cut(s) 86, 91, 903, 975, 2756, 2856
MmeI TCCRAC 9 cut(s) 219, 295, 353, 736, 985, 2129, 2848, 3219, 3766
Mox20I TGGCCA 1 cut(s) 3881
MscI TGGCCA 1 cut(s) 3881
MseI TTAA 5 cut(s) 356, 420, 1434, 1698, 1899
MslI CAYNNNNRTG 5 cut(s) 357, 1844, 2597, 3473, 3494
Msp20I TGGCCA 1 cut(s) 3881
MspI CCGG 4 cut(s) 57, 582, 2782, 3504
MspR9I CCNGG 7 cut(s) 546, 1455, 2547, 2782, 2783, 2877, 3232
MunI CAATTG 3 cut(s) 2919, 3000, 3636
Mva1269I GAATGC 2 cut(s) 147, 2119
MvaI CCWGG 5 cut(s) 546, 1455, 2547, 2877, 3232
MwoI GCNNNNNNNGC 9 cut(s) 349, 737, 1124, 1514, 2776, 2993, 3147, 3263, 3500
NciI CCSGG 2 cut(s) 2782, 2783
NcoI CCATGG 3 cut(s) 1799, 3780, 3862
NdeI CATATG 2 cut(s) 1332, 1719
NmuCI GTSAC 4 cut(s) 1294, 1726, 2734, 2855
NspI RCATGY 1 cut(s) 2159
OliI CACNNNNGTG 1 cut(s) 3473
PaeR7I CTCGAG 2 cut(s) 2499, 4061
PagI TCATGA 1 cut(s) 9
PceI AGGCCT 2 cut(s) 1342, 2545
PciI ACATGT 1 cut(s) 2155
PciSI GCTCTTC 1 cut(s) 27
PctI GAATGC 2 cut(s) 147, 2119
PfeI GAWTC 3 cut(s) 208, 2792, 4076
PleI GAGTC 6 cut(s) 85, 90, 903, 975, 2755, 2856
PpsI GAGTC 6 cut(s) 85, 90, 903, 975, 2755, 2856
Ppu21I YACGTR 2 cut(s) 126, 2103
PpuMI RGGWCCY 1 cut(s) 1208
PscI ACATGT 1 cut(s) 2155
PshBI ATTAAT 1 cut(s) 420
Psp5II RGGWCCY 1 cut(s) 1208
Psp6I CCWGG 5 cut(s) 544, 1453, 2545, 2875, 3230
PspFI CCCAGC 1 cut(s) 2582
PspGI CCWGG 5 cut(s) 544, 1453, 2545, 2875, 3230
PspOMI GGGCCC 1 cut(s) 539
PspPI GGNCC 9 cut(s) 473, 539, 540, 758, 1007, 1208, 1759, 2224, 3140
PspPPI RGGWCCY 1 cut(s) 1208
PstI CTGCAG 2 cut(s) 1137, 3475
PsuI RGATCY 5 cut(s) 820, 1812, 3529, 3559, 3729
RseI CAYNNNNRTG 5 cut(s) 357, 1844, 2597, 3473, 3494
SapI GCTCTTC 1 cut(s) 27
SaqAI TTAA 5 cut(s) 356, 420, 1434, 1698, 1899
Sau96I GGNCC 9 cut(s) 473, 539, 540, 758, 1007, 1208, 1759, 2224, 3140
ScaI AGTACT 4 cut(s) 1020, 2337, 3349, 3510
SchI GAGTC 6 cut(s) 86, 91, 903, 975, 2756, 2856
ScrFI CCNGG 7 cut(s) 546, 1455, 2547, 2782, 2783, 2877, 3232
SduI GDGCHC 4 cut(s) 543, 1330, 1966, 3600
SfaNI GCATC 6 cut(s) 401, 2313, 2360, 2974, 3076, 3275
SfcI CTRYAG 6 cut(s) 1133, 1861, 3045, 3471, 3909, 4089
SfiI GGCCNNNNNGGCC 1 cut(s) 3147
Sfr274I CTCGAG 2 cut(s) 2499, 4061
SinI GGWCC 5 cut(s) 473, 758, 1007, 1208, 1759
SlaI CTCGAG 2 cut(s) 2499, 4061
SmaI CCCGGG 1 cut(s) 2783
SmiI ATTTAAAT 1 cut(s) 357
SmiMI CAYNNNNRTG 5 cut(s) 357, 1844, 2597, 3473, 3494
SmlI CTYRAG 6 cut(s) 287, 896, 1226, 2318, 2499, 4061
SmoI CTYRAG 6 cut(s) 287, 896, 1226, 2318, 2499, 4061
SnaBI TACGTA 1 cut(s) 126
SseBI AGGCCT 2 cut(s) 1342, 2545
SsiI CCGC 4 cut(s) 301, 343, 1264, 2227
SspI AATATT 4 cut(s) 811, 877, 2955, 3392
StuI AGGCCT 2 cut(s) 1342, 2545
StyD4I CCNGG 7 cut(s) 544, 1453, 2545, 2780, 2781, 2875, 3230
StyI CCWWGG 5 cut(s) 576, 1799, 2292, 3780, 3862
SwaI ATTTAAAT 1 cut(s) 357
TaiI ACGT 7 cut(s) 9, 128, 566, 766, 1098, 1836, 2105
TatI WGTACW 8 cut(s) 1018, 1239, 2011, 2335, 3347, 3375, 3508, 3687
TfiI GAWTC 3 cut(s) 208, 2792, 4076
Tru1I TTAA 5 cut(s) 356, 420, 1434, 1698, 1899
Tru9I TTAA 5 cut(s) 356, 420, 1434, 1698, 1899
TseFI GTSAC 4 cut(s) 1294, 1726, 2734, 2855
Tsp45I GTSAC 4 cut(s) 1294, 1726, 2734, 2855
TspGWI ACGGA 1 cut(s) 1125
TspMI CCCGGG 1 cut(s) 2781
VpaK11BI GGWCC 5 cut(s) 473, 758, 1007, 1208, 1759
VspI ATTAAT 1 cut(s) 420
XagI CCTNNNNNAGG 1 cut(s) 1289
XceI RCATGY 1 cut(s) 2159
XcmI CCANNNNNNNNNTGG 2 cut(s) 497, 1937
XhoI CTCGAG 2 cut(s) 2499, 4061
XmaI CCCGGG 1 cut(s) 2781
XmiI GTMKAC 2 cut(s) 1554, 3610
ZrmI AGTACT 4 cut(s) 1020, 2337, 3349, 3510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.