Rh6BG403500
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000493
Physical Location & Seq
Forward (+)
63034934 .. 63041582
6649 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG403500.1

Sequence Viewer

Length: 4257 bp
ATGGAAGTGTTTTCGAGGTCTTTTCGGGGTGAAGACGATGAAGAAGCTCTCAAATGGGCTGCAATTGAGAGGCTGCCTACTGGTTTGCGTATCGGGAGAGGCTTGTTCATTGATGGTGAGGGCCAAGCTAGGGAGGTTGGTGTCGAAAACCTTGGTTTACTTGAGAGGAAGACTTTGTTGGAGAGGTTGGTGAAAAATGCAGAGAAAGATAATGAGAAGTTCTTGTTAAAGCTCAAGAACCGAATTGACAGAGTTAAACTTCATATGCCCACAATTGAAGTTCGGTTTGAGCATCTGACTGTGGAGGCAAAAGTTTATTTGGGAAGCAGAGCACTGCCTACGTTACTCAACTTTGCCATCAATACGTTACAGGGATGGTTACATTATCTTCACATTTTTCCAAGTAGAAAGGCATCCTTAACAATCCTTCAAAATGTTAGTGGCATTATCAAACCCCAAAGAATGACTTTGCTTTTAGGTCCCCCAAGTTCCGGAAAAACCACATTACTATTGGCTCTTGCAGGAAGACTTGGTAAAGATTTAAGATCTTCGGGAGAAGTTACGTATAATGGACATGGTATGGCAGAATTTGTACCTCAGAGAACATCAGCTTATATAAGTCAAAATGATCTTCACATAGGAGAAATGACAGTGAAAGAGACACTGGCTTTCTCAGCTAGATGTCAAGGAGTTGGATCAAATTGCGACATGTTGGCAGAATTGTCTAGAAGAGAGAAGGAGGAAAATATCAAGCCTGATCCCGATATTGATATCTACCAAAAGGCAGCAGCATTAGAAGGGCAGGAGAGTAGTGTAGTTGCTGATTATATTCTCAAGATTTTGGGACTTGAAGTTTGTGCCAATACTATGGTGGGGGATGAAATGGTAAGAGGCATCTCGGGTGGAGAAAGAAAGCGAGTCACAATAGGGGAGATGCTTGTTGGACCAGTAAGAGCTCTCTTCATGGATGAGATATCAACTGGCTTGGACAGTTCAACAACTTTCCAGATAGTAAACTCACTCAGGCAATCAATCCATATTCTTAGGGGAACTGCAGTTGTTTCCCTCCTACAGCCAGCCCCTGAAACTTATAACCTATTCGATGATATAATTCTCCTATCAGATGGCCAAATAGTGTATCAGGGTCCACGTGAAAGTGTGCTCGACTTTTTTGAATTCATGGGGTTCAAATGTCCTGAGAGGAAAGGAGTTGCTGACTTCTTGCAAGAGGTGACATCCAGGAAAGATCAAGCACAGTATTGGGCACATTTACATAAGCCTTACAGATTTGTTACTGTCACTGAGTTTGCTGAAGCATTCCAGGCATTTCATATTGGCCAGAGACTTGGTGATGAGCTTGCTAGTCCCTTCGACAGGTCTAAAAGCCACCGTGCTGCTTTAACAGCTAAGAAATATGGTGTTAACAAGAAGGAGTTGTTCAGAGCTTGCGTTTCTAGAGAGTTTTTGCTCATGAAGAGGAACTCATTTGCCTACATTTTCAAACTGGCTCTACTTATCATCTTAGCTTCTATAACAACAACAATATTTCTAAGAGTTGAGTTGCATAGAGGCACTGTGTTGGATGGGGGGATCTATCTGGGTGCTTTGTTCTTTGCAGTCATTGTAGCGATGTTTAATGGAATATCAGAGCCGAACATGGCCATTATGAAACTTCCCGTCTTTTACAAACAAAGAGACCTCCTCTTCTATCCTGCATGGGCATACTCGATACCTGGATGGATACTCAAGATCCCAATCACTTTAATAGAGAGTTTCATTTGGGTGATCATTACTTATTATGTCATAGGATTTGATCCACATGTTGAAAGGTTCCTCAAGCAGTACATGTTACTGGTGTGCATAAACCAGATGGCGTCCGGGTTGTTCAGGTCCATGGCAGCAATAGGAAGGAGTGTAATAGTTGCAAGCACATTTGGAACATTTGCATTACTTATAATCTTGGTTTTGGGTGGATTTGTCTTATCTCGAGAGGCTGTACCAAAATGGTGGTTGTGGGGGTATTGGGTCTCGCCGTTGATGTATGGACAAAATGCCATAGCTGTGAATGAATTTCTTGGAAAGAGTTGGAGCCATGTTCCACCTGGTTCTACAGAATCCTTGGGAGTTATGGTCCTCAAGTCTCATGGCATTTTCCCAGAAGCACATTGGTATTGGATAGGAGTCGCAGCCTTAGTTGGATACACTTTTCTGTTCAACATCATCATCATTTTCGCTCTACAATACCTTGATCCCTTTGGGAAGCCTCAGGCAGTACTGTCCAAAGAAAGCATAGCGGAGAGAAATGGTAATGGAACTGGAGAGTTCATTGAGCTATTATCAAGAGGAAATCGATATTCTGAAAGTAGTATTGAAGGTGCAACAAGCTTGTCATCATCTACAAGTGGAGGAAGAAGTGATAATGCTAATGGGAAAAGGAAGAGAGGAATGGTTCTTCCTTTTCAACCTCTTTCCCTTGCTTTCGATGAGATCAGATATGCCATAGACATGCCACAGGAAATGAAAGCTCAGGGTGTTATGGAGGATAGGCTGGAACTGTTGAAAGGGGTAAGTGGTGCTTTTAGACCAGGAGTCTTAACAGCCCTGATGGGTGTTAGTGGTGCAGGCAAGACCACTCTAATGGATGTCTTGGCCGGAAGGAAAACTGGTGGATACATTGAGGGAAGCATCGTGATATCTGGGTACTGTAAAAGGCAAGAAACATTTGCTCGCATATCAGGTTATTGCGAGCAAACTGATATCCACTCTCCTCATGTTACAGTCTATGAATCCCTGCTGTTTTCTGCTTGGCTTCGGCTACTGCCTGAGGTTGATTCTGCAACTAGGACGATGTTTATCGAGGAAGTTATGGAGCTTGTGGAGCTGACTTCACTTAGGGGAGCACTTGTGGGATTACCAGGTGTAACTGGCCTTTCAACTGAGCAGCGCAAAAGACTGACTATTGCCGTTGAGCTTGTTGCAAATCCATCTATAATTTTCATGGATGAGCCAACATCTGGCCTTGATGCCAGGGCAGCAGCAATAGTGATGAGAACAGTTAGGAACACAGTGGACACAGGCCGAACTGTGGTTTGCACGATCCACCAGCCTAGCATAGATATATTTGAAGCTTTTGATGAGCTTCTTCTTCTCAAATTGGGAGGTGAGCCCATATACTCCGGTCCATTAGGCCTCCATTCCTCCTTTCTGATTGAGTACTTTGAGGGAATTAATGGCGTTCCTAAAGTTAAAGATGGATATAACCCTGCAACCTGGATGTTGGAAATTACTTCAGCAGCACAAGAGGAAGCTCTTGGGGTCAACTTCACAGATGTGTACAAGAAATCAGAACCATATAAGAGAACTAAAGATATGATCAAGGAATTGTCTATACCTCCCTCAGGTTCTAAGGATCTGTACTTTCCTACTCGCTACTCACAATCTTTTCACACCCAATGCATGGCTTGCCTATGGAAACAACACTGGTCGTACTGGCGAAACCCATCATATAGTGCAGTTAGACTCTTATTCACAACATTCATCGCGTTGTTGTTTGGTATGATATTTTGGGATCTCGGGTCAAAACGGAGCAAGCAGCAAGACATCTTCAATGCAATGGGCTCTATATATTCTGCTGTTATTTTTCTCGGGGTCCAGAATGCTGCCTCAGTGCAGCCAATTGTGGCTGTTGAGAGAACAGTGTTTTACAGAGAAAGAGCAGCTGGTATGTACTCAGCTTTGCCTTACGCATTTGGACAGATGATGATTGAGGTTCCCTACATTTTCATTCAGACAATTATTTATGGGGTTATAGTGGGATTCGATTGGATAGTTTCCAAGTTCCTTTGGTACCTTTTCTTCATGTACTTCACCTTATTATACTTCACATTCTATGGCATGATGACCATGGCTCTAACTCCCAACCACAACATTGCTGCTATAACTTCATCTTCCTTCTATGCAATATGGAACCTTTTCTCAGGATTTGTCATTCCTCCATCGAGAATACCTATATGGTGGAAATGGTACTATTGGATTTGCCCTGTTGCTTGGACCTTGTATGGAATGGCCGCTTCGCAGTTTGGAGACATCAAGCAGAGACTCGAGACTGGCGAAACTGTGGAAGATTTTTTGACAAGCTACTTTGGATACAGGCACGACTTTCTGGGTATCGTTGCAGTGGTGGTTGTCGGGTTTTCATTGCTGTTCGGCTTTATCTTTGCTTATTCCATTCGAGCTTTTAACTTCCAAAAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1418

Amino Acids

159.34

Weight (kDa)

8.28

Isoelectric Point (pI)

38.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 67 - 118 1.5e-07 ABC-transporter N-terminal
ABC_tran PF00005 143 - 324 1.5e-15 ABC transporter
ABC2_membrane PF01061 479 - 691 2.2e-47 ABC-2 type transporter
PDR_assoc PF08370 696 - 758 1.3e-27 Plant PDR ABC transporter associated
ABC_tran PF00005 853 - 1005 7.4e-19 ABC transporter
ABC2_membrane PF01061 1150 - 1360 1.2e-53 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000493)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29820 FvH4_2g29820 FvH4_2g29822 FvH4_2g29824 FvH4_2g29824 FvH4_2g29824 FvH4_2g29840 FvH4_2g29840 FvH4_2g29840 FvH4_5g00550 FvH4_5g00550
malus_domestica MD02G1032700.v1.1 MD08G1140900.v1.1 MD08G1141400.v1.1 MD15G1117800.v1.1 MD15G1117900.v1.1
prunus_persica Prupe.1G471200_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471400_v2.0.a1 Prupe.7G242900_v2.0.a1
pyrus_communis pycom02g02690 pycom08g11890
rosa_chinensis RchiOBHm_Chr2g0111741 RchiOBHm_Chr2g0145991 RchiOBHm_Chr6g0298851 RchiOBHm_Chr6g0298871 RchiOBHm_Chr6g0298891 RchiOBHm_Chr6g0298901 RchiOBHm_Chr6g0298911 RchiOBHm_Chr7g0201801 RchiOBHm_Chr7g0211531 RchiOBHm_Chr7g0214601
rosa_laevigata RLG00000002931 RLG00000003000 RLG00000003647 RLG00000011476 RLG00000011479
rosa_multiflora Rmu_sc0001030.1_g000012 Rmu_sc0001548.1_g000041 Rmu_ssc0000397.1_g000033 Rmu_ssc0000397.1_g000036 Rmu_ssc0000397.1_g000037 Rmu_ssc0000397.1_g000039 Rmu_ssc0000397.1_g000048
rosa_roxburghii Rroxscaffold_1G00003830 Rroxscaffold_3G00247810 Rroxscaffold_3G00255060 Rroxscaffold_7G00169220 Rroxscaffold_7G00169230 Rroxscaffold_7G00169280
rosa_rugosa Rorug02G0223600 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283200 Rorug06G0283300 Rorug06G0283400 Rorug06G0283500 Rorug07G0066000
rosa_samantha Rh2AG511300 Rh2CG235100 Rh2CG496400 Rh2DG330900 Rh6BG403100 Rh6BG403300 Rh6BG403400 Rh6BG403500 Rh6CG408700 Rh6CG408900 Rh6CG409000 Rh6CG409200 Rh6DG395800 Rh6DG395900 Rh6DG396000 Rh6DG396200 Rh7AG193400 Rh7BG195100 Rh7BG436600 Rh7DG199300 Rh7DG265500
rosa_wichuraiana Rw2G016900 Rw6G034570 Rw6G034580 Rw6G034590 Rw6G034600 Rw7G016900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1092, 1955
Acc65I GGTACC 1 cut(s) 3846
AccB1I GGYRCC 1 cut(s) 3846
AccB7I CCANNNNNTGG 2 cut(s) 3014, 3457
AccII CGCG 1 cut(s) 3542
AccIII TCCGGA 1 cut(s) 491
AciI CCGC 2 cut(s) 2294, 4068
AclWI GGATC 9 cut(s) 703, 752, 1598, 1744, 1808, 2243, 3091, 3417, 3576
AcoI YGGCCR 5 cut(s) 1126, 1336, 1659, 2649, 4065
AcsI RAATTY 3 cut(s) 587, 1175, 2069
AcuI CTGAAG 2 cut(s) 1332, 3273
AcvI CACGTG 1 cut(s) 1151
AcyI GRCGYC 1 cut(s) 1874
AfiI CCNNNNNNNGG 7 cut(s) 130, 491, 1374, 3014, 3085, 3398, 3457
AflIII ACRYGT 3 cut(s) 708, 1819, 1845
AhdI GACNNNNNGTC 1 cut(s) 2588
AjnI CCWGG 8 cut(s) 1238, 1320, 1732, 2101, 2584, 2914, 3026, 3269
AjuI GAANNNNNNNTTGG 2 cut(s) 161, 193
AleI CACNNNNGTG 1 cut(s) 3329
Alw21I GWGCWC 4 cut(s) 334, 958, 1164, 2902
Alw26I GTCTC 8 cut(s) 653, 1336, 1689, 2032, 2145, 4077, 4090, 4097
AlwI GGATC 9 cut(s) 703, 752, 1598, 1744, 1808, 2243, 3091, 3417, 3576
AlwNI CAGNNNCTG 1 cut(s) 1082
Ama87I CYCGRG 5 cut(s) 898, 1986, 3572, 3644, 4100
Aor13HI TCCGGA 1 cut(s) 491
ApoI RAATTY 3 cut(s) 587, 1175, 2069
ArsI GACNNNNNNTTYG 2 cut(s) 837, 869
AseI ATTAAT 1 cut(s) 3228
Asp700I GAANNNNTTC 1 cut(s) 3971
Asp718I GGTACC 1 cut(s) 3846
AspLEI GCGC 1 cut(s) 2946
AspS9I GGNCC 9 cut(s) 121, 479, 944, 1145, 1890, 2131, 3179, 3649, 4050
AsuC2I CCSGG 1 cut(s) 1879
AsuHPI GGTGA 8 cut(s) 41, 128, 202, 1243, 1361, 1795, 3173, 3859
AvaI CYCGRG 5 cut(s) 898, 1986, 3572, 3644, 4100
AvaII GGWCC 8 cut(s) 479, 944, 1145, 1890, 2131, 3179, 3649, 4050
AxyI CCTNAGG 3 cut(s) 2265, 2823, 3397
BaeGI GKGCMC 1 cut(s) 1267
BalI TGGCCA 3 cut(s) 1128, 1338, 1661
BanI GGYRCC 1 cut(s) 3846
BanII GRGCYC 3 cut(s) 958, 3168, 3620
BarI GAAGNNNNNNTAC 2 cut(s) 3839, 3871
BbrPI CACGTG 1 cut(s) 1151
BbsI GAAGAC 3 cut(s) 39, 176, 532
Bbv12I GWGCWC 4 cut(s) 334, 958, 1164, 2902
BceAI ACGGC 2 cut(s) 2017, 2948
BciT130I CCWGG 8 cut(s) 1240, 1322, 1734, 2103, 2586, 2916, 3028, 3271
BciVI GTATCC 4 cut(s) 1734, 2192, 2663, 4139
BclI TGATCA 2 cut(s) 1785, 3372
BcnI CCSGG 1 cut(s) 1879
BcoDI GTCTC 8 cut(s) 653, 1336, 1689, 2032, 2145, 4077, 4090, 4097
BfaI CTAG 7 cut(s) 129, 678, 726, 1362, 1455, 2841, 3108
BfmI CTRYAG 3 cut(s) 1053, 1070, 2109
BfuI GTATCC 4 cut(s) 1734, 2192, 2663, 4139
BglII AGATCT 1 cut(s) 545
BmcAI AGTACT 2 cut(s) 2274, 3215
Bme1390I CCNGG 9 cut(s) 1240, 1322, 1734, 1879, 2103, 2586, 2916, 3028, 3271
Bme18I GGWCC 8 cut(s) 479, 944, 1145, 1890, 2131, 3179, 3649, 4050
BmeRI GACNNNNNGTC 1 cut(s) 2588
BmeT110I CYCGRG 5 cut(s) 898, 1986, 3572, 3644, 4100
BmgT120I GGNCC 9 cut(s) 121, 479, 944, 1145, 1890, 2131, 3179, 3649, 4050
BmiI GGNNCC 8 cut(s) 481, 1146, 1832, 2090, 3650, 3771, 3848, 3968
BmrFI CCNGG 9 cut(s) 1240, 1322, 1734, 1879, 2103, 2586, 2916, 3028, 3271
BmsI GCATC 6 cut(s) 301, 422, 903, 924, 2694, 3013
BpiI GAAGAC 3 cut(s) 39, 176, 532
BplI GAGNNNNNCTC 2 cut(s) 1686, 1718
BpmI CTGGAG 1 cut(s) 2337
Bpu10I CCTNAGC 1 cut(s) 2526
BpuEI CTTGAG 6 cut(s) 182, 218, 818, 1730, 1820, 2120
BpuMI CCSGG 1 cut(s) 1879
Bsa29I ATCGAT 1 cut(s) 2350
BsaAI YACGTR 2 cut(s) 564, 1151
BsaBI GATNNNNATC 2 cut(s) 1754, 2852
BsaHI GRCGYC 1 cut(s) 1874
BsaI GGTCTC 2 cut(s) 1689, 2032
BsaJI CCNNGG 5 cut(s) 151, 1893, 2118, 3027, 3903
BsaWI WCCGGW 2 cut(s) 491, 3176
BsaXI ACNNNNNCTCC 4 cut(s) 2115, 2145, 2889, 2919
Bsc4I CCNNNNNNNGG 7 cut(s) 130, 491, 1374, 3014, 3085, 3398, 3457
Bse21I CCTNAGG 3 cut(s) 2265, 2823, 3397
Bse3DI GCAATG 3 cut(s) 3618, 3927, 4196
Bse8I GATNNNNATC 2 cut(s) 1754, 2852
BseAI TCCGGA 1 cut(s) 491
BseBI CCWGG 8 cut(s) 1240, 1322, 1734, 2103, 2586, 2916, 3028, 3271
BseCI ATCGAT 1 cut(s) 2350
BseDI CCNNGG 5 cut(s) 151, 1893, 2118, 3027, 3903
BseJI GATNNNNATC 2 cut(s) 1754, 2852
BseLI CCNNNNNNNGG 7 cut(s) 130, 491, 1374, 3014, 3085, 3398, 3457
BseMI GCAATG 3 cut(s) 3618, 3927, 4196
BseRI GAGGAG 2 cut(s) 1691, 2757
BseSI GKGCMC 1 cut(s) 1267
BsgI GTGCAG 3 cut(s) 2640, 3531, 3689
Bsh1236I CGCG 1 cut(s) 3542
BshNI GGYRCC 1 cut(s) 3846
BshVI ATCGAT 1 cut(s) 2350
BsiHKAI GWGCWC 4 cut(s) 334, 958, 1164, 2902
BsiHKCI CYCGRG 5 cut(s) 898, 1986, 3572, 3644, 4100
BsiSI CCGG 4 cut(s) 492, 1878, 2652, 3177
BslFI GGGAC 3 cut(s) 465, 858, 1350
BslI CCNNNNNNNGG 7 cut(s) 130, 491, 1374, 3014, 3085, 3398, 3457
BsmAI GTCTC 8 cut(s) 653, 1336, 1689, 2032, 2145, 4077, 4090, 4097
BsmFI GGGAC 3 cut(s) 465, 858, 1350
BsmI GAATGC 2 cut(s) 1316, 3661
Bso31I GGTCTC 2 cut(s) 1689, 2032
BsoBI CYCGRG 5 cut(s) 898, 1986, 3572, 3644, 4100
Bsp1286I GDGCHC 7 cut(s) 334, 958, 1164, 1267, 2902, 3168, 3620
Bsp13I TCCGGA 1 cut(s) 491
Bsp1407I TGTACA 1 cut(s) 3333
Bsp19I CCATGG 2 cut(s) 1893, 3903
BspACI CCGC 2 cut(s) 2294, 4068
BspDI ATCGAT 1 cut(s) 2350
BspEI TCCGGA 1 cut(s) 491
BspFNI CGCG 1 cut(s) 3542
BspHI TCATGA 1 cut(s) 1470
BspLI GGNNCC 8 cut(s) 481, 1146, 1832, 2090, 3650, 3771, 3848, 3968
BspMAI CTGCAG 1 cut(s) 1057
BspPI GGATC 9 cut(s) 703, 752, 1598, 1744, 1808, 2243, 3091, 3417, 3576
BspT107I GGYRCC 1 cut(s) 3846
BspTNI GGTCTC 2 cut(s) 1689, 2032
BsrDI GCAATG 3 cut(s) 3618, 3927, 4196
BsrGI TGTACA 1 cut(s) 3333
BssECI CCNNGG 5 cut(s) 151, 1893, 2118, 3027, 3903
BssNI GRCGYC 1 cut(s) 1874
BssT1I CCWWGG 4 cut(s) 151, 1893, 2118, 3903
Bst2UI CCWGG 8 cut(s) 1240, 1322, 1734, 2103, 2586, 2916, 3028, 3271
Bst6I CTCTTC 5 cut(s) 724, 965, 1469, 1709, 2432
BstACI GRCGYC 1 cut(s) 1874
BstAPI GCANNNNNTGC 1 cut(s) 3462
BstAUI TGTACA 1 cut(s) 3333
BstBAI YACGTR 2 cut(s) 564, 1151
BstC8I GCNNGC 9 cut(s) 1077, 1359, 1447, 1927, 2623, 2728, 2747, 3463, 3590
BstDSI CCRYGG 2 cut(s) 1893, 3903
BstENI CCTNNNNNAGG 2 cut(s) 1372, 3396
BstFNI CGCG 1 cut(s) 3542
BstHHI GCGC 1 cut(s) 2946
BstMAI GTCTC 8 cut(s) 653, 1336, 1689, 2032, 2145, 4077, 4090, 4097
BstMWI GCNNNNNNNGC 6 cut(s) 674, 1322, 1403, 2878, 3032, 3462
BstNI CCWGG 8 cut(s) 1240, 1322, 1734, 2103, 2586, 2916, 3028, 3271
BstNSI RCATGY 4 cut(s) 712, 1823, 1849, 2509
BstSCI CCNGG 9 cut(s) 1238, 1320, 1732, 1877, 2101, 2584, 2914, 3026, 3269
BstSFI CTRYAG 3 cut(s) 1053, 1070, 2109
BstSLI GKGCMC 1 cut(s) 1267
BstSNI TACGTA 1 cut(s) 564
BstUI CGCG 1 cut(s) 3542
BstV2I GAAGAC 3 cut(s) 39, 176, 532
BstX2I RGATCY 5 cut(s) 545, 1590, 1749, 3409, 3568
BstXI CCANNNNNNTGG 4 cut(s) 868, 1346, 2007, 2638
BstYI RGATCY 5 cut(s) 545, 1590, 1749, 3409, 3568
Bsu15I ATCGAT 1 cut(s) 2350
Bsu36I CCTNAGG 3 cut(s) 2265, 2823, 3397
BsuI GTATCC 4 cut(s) 1734, 2192, 2663, 4139
BsuTUI ATCGAT 1 cut(s) 2350
BtgI CCRYGG 2 cut(s) 1893, 3903
BtgZI GCGATG 2 cut(s) 1643, 3523
BtsI GCAGTG 2 cut(s) 332, 4182
Cac8I GCNNGC 9 cut(s) 1077, 1359, 1447, 1927, 2623, 2728, 2747, 3463, 3590
CaiI CAGNNNCTG 1 cut(s) 1082
CciI TCATGA 1 cut(s) 1470
CfoI GCGC 1 cut(s) 2946
Cfr13I GGNCC 9 cut(s) 121, 479, 944, 1145, 1890, 2131, 3179, 3649, 4050
ClaI ATCGAT 1 cut(s) 2350
CseI GACGC 1 cut(s) 1863
CsiI ACCWGGT 2 cut(s) 2101, 2914
DriI GACNNNNNGTC 1 cut(s) 2588
EaeI YGGCCR 5 cut(s) 1126, 1336, 1659, 2649, 4065
Eam1104I CTCTTC 5 cut(s) 724, 965, 1469, 1709, 2432
Eam1105I GACNNNNNGTC 1 cut(s) 2588
EarI CTCTTC 5 cut(s) 724, 965, 1469, 1709, 2432
Ecl136II GAGCTC 1 cut(s) 956
Eco105I TACGTA 1 cut(s) 564
Eco130I CCWWGG 4 cut(s) 151, 1893, 2118, 3903
Eco147I AGGCCT 1 cut(s) 3189
Eco24I GRGCYC 3 cut(s) 958, 3168, 3620
Eco31I GGTCTC 2 cut(s) 1689, 2032
Eco32I GATATC 4 cut(s) 772, 975, 2694, 2758
Eco47I GGWCC 8 cut(s) 479, 944, 1145, 1890, 2131, 3179, 3649, 4050
Eco53kI GAGCTC 1 cut(s) 956
Eco57I CTGAAG 2 cut(s) 1332, 3273
Eco72I CACGTG 1 cut(s) 1151
Eco81I CCTNAGG 3 cut(s) 2265, 2823, 3397
Eco88I CYCGRG 5 cut(s) 898, 1986, 3572, 3644, 4100
EcoICRI GAGCTC 1 cut(s) 956
EcoNI CCTNNNNNAGG 2 cut(s) 1372, 3396
EcoO109I RGGNCCY 1 cut(s) 479
EcoRI GAATTC 1 cut(s) 1175
EcoRII CCWGG 8 cut(s) 1238, 1320, 1732, 2101, 2584, 2914, 3026, 3269
EcoRV GATATC 4 cut(s) 772, 975, 2694, 2758
EcoT14I CCWWGG 4 cut(s) 151, 1893, 2118, 3903
EcoT22I ATGCAT 1 cut(s) 3458
EcoT38I GRGCYC 3 cut(s) 958, 3168, 3620
ErhI CCWWGG 4 cut(s) 151, 1893, 2118, 3903
FalI AAGNNNNNCTT 6 cut(s) 401, 433, 451, 483, 2560, 2592
FaqI GGGAC 3 cut(s) 465, 858, 1350
FauNDI CATATG 1 cut(s) 264
FbaI TGATCA 2 cut(s) 1785, 3372
FriOI GRGCYC 3 cut(s) 958, 3168, 3620
FspBI CTAG 7 cut(s) 129, 678, 726, 1362, 1455, 2841, 3108
GlaI GCGC 1 cut(s) 2945
GsuI CTGGAG 1 cut(s) 2337
HapII CCGG 4 cut(s) 492, 1878, 2652, 3177
HgaI GACGC 1 cut(s) 1863
HhaI GCGC 1 cut(s) 2946
Hin1I GRCGYC 1 cut(s) 1874
Hin6I GCGC 1 cut(s) 2944
HinP1I GCGC 1 cut(s) 2944
HincII GTYRAC 2 cut(s) 1423, 3319
HindII GTYRAC 2 cut(s) 1423, 3319
HindIII AAGCTT 2 cut(s) 2383, 3126
HinfI GANTC 9 cut(s) 918, 2114, 2181, 2589, 2786, 2831, 3519, 3816, 4098
HpaI GTTAAC 1 cut(s) 1423
HpaII CCGG 4 cut(s) 492, 1878, 2652, 3177
HphI GGTGA 8 cut(s) 41, 128, 202, 1243, 1361, 1795, 3173, 3859
Hpy166II GTNNAC 7 cut(s) 158, 1015, 1148, 1423, 3070, 3319, 3334
Hpy8I GTNNAC 7 cut(s) 158, 1015, 1148, 1423, 3070, 3319, 3334
HpyAV CCTTC 9 cut(s) 437, 730, 791, 1378, 1423, 1902, 2366, 2649, 3961
HpyCH4IV ACGT 4 cut(s) 341, 365, 563, 1150
HpyF10VI GCNNNNNNNGC 6 cut(s) 674, 1322, 1403, 2878, 3032, 3462
HpySE526I ACGT 4 cut(s) 341, 365, 563, 1150
Hsp92I GRCGYC 1 cut(s) 1874
HspAI GCGC 1 cut(s) 2944
Kpn2I TCCGGA 1 cut(s) 491
KpnI GGTACC 1 cut(s) 3850
Ksp22I TGATCA 2 cut(s) 1785, 3372
KspAI GTTAAC 1 cut(s) 1423
LmnI GCTCC 5 cut(s) 2088, 2869, 2878, 2897, 3585
LweI GCATC 6 cut(s) 301, 422, 903, 924, 2694, 3013
MabI ACCWGGT 2 cut(s) 2101, 2914
MaeI CTAG 7 cut(s) 129, 678, 726, 1362, 1455, 2841, 3108
MaeII ACGT 4 cut(s) 341, 365, 563, 1150
MfeI CAATTG 3 cut(s) 63, 273, 3675
MflI RGATCY 5 cut(s) 545, 1590, 1749, 3409, 3568
MhlI GDGCHC 7 cut(s) 334, 958, 1164, 1267, 2902, 3168, 3620
MlsI TGGCCA 3 cut(s) 1128, 1338, 1661
MluNI TGGCCA 3 cut(s) 1128, 1338, 1661
MlyI GAGTC 5 cut(s) 927, 2190, 2598, 3513, 4092
MmeI TCCRAC 7 cut(s) 159, 673, 922, 1560, 2066, 2176, 3258
Mox20I TGGCCA 3 cut(s) 1128, 1338, 1661
Mph1103I ATGCAT 1 cut(s) 3458
MroI TCCGGA 1 cut(s) 491
MroXI GAANNNNTTC 1 cut(s) 3971
MscI TGGCCA 3 cut(s) 1128, 1338, 1661
MslI CAYNNNNRTG 5 cut(s) 1781, 2060, 2504, 2636, 3329
Msp20I TGGCCA 3 cut(s) 1128, 1338, 1661
MspA1I CMGCKG 1 cut(s) 3719
MspI CCGG 4 cut(s) 492, 1878, 2652, 3177
MspR9I CCNGG 9 cut(s) 1240, 1322, 1734, 1879, 2103, 2586, 2916, 3028, 3271
MunI CAATTG 3 cut(s) 63, 273, 3675
Mva1269I GAATGC 2 cut(s) 1316, 3661
MvaI CCWGG 8 cut(s) 1240, 1322, 1734, 2103, 2586, 2916, 3028, 3271
MvnI CGCG 1 cut(s) 3542
MwoI GCNNNNNNNGC 6 cut(s) 674, 1322, 1403, 2878, 3032, 3462
NciI CCSGG 1 cut(s) 1879
NcoI CCATGG 2 cut(s) 1893, 3903
NdeI CATATG 1 cut(s) 264
NlaIV GGNNCC 8 cut(s) 481, 1146, 1832, 2090, 3650, 3771, 3848, 3968
NmuCI GTSAC 3 cut(s) 919, 1231, 1297
NsiI ATGCAT 1 cut(s) 3458
NspI RCATGY 4 cut(s) 712, 1823, 1849, 2509
OliI CACNNNNGTG 1 cut(s) 3329
PaeR7I CTCGAG 2 cut(s) 1986, 4100
PagI TCATGA 1 cut(s) 1470
PceI AGGCCT 1 cut(s) 3189
PciI ACATGT 3 cut(s) 708, 1819, 1845
PcsI WCGNNNNNNNCGW 1 cut(s) 4107
PctI GAATGC 2 cut(s) 1316, 3661
PdmI GAANNNNTTC 1 cut(s) 3971
PfeI GAWTC 4 cut(s) 2114, 2786, 2831, 3816
PflMI CCANNNNNTGG 2 cut(s) 3014, 3457
PfoI TCCNGGA 1 cut(s) 1238
PleI GAGTC 5 cut(s) 926, 2189, 2597, 3513, 4092
PmaCI CACGTG 1 cut(s) 1151
PmlI CACGTG 1 cut(s) 1151
PpsI GAGTC 5 cut(s) 926, 2189, 2597, 3513, 4092
Ppu21I YACGTR 2 cut(s) 564, 1151
PpuMI RGGWCCY 1 cut(s) 479
PscI ACATGT 3 cut(s) 708, 1819, 1845
PshBI ATTAAT 1 cut(s) 3228
PsiI TTATAA 2 cut(s) 1092, 1955
Psp124BI GAGCTC 1 cut(s) 958
Psp5II RGGWCCY 1 cut(s) 479
Psp6I CCWGG 8 cut(s) 1238, 1320, 1732, 2101, 2584, 2914, 3026, 3269
PspCI CACGTG 1 cut(s) 1151
PspGI CCWGG 8 cut(s) 1238, 1320, 1732, 2101, 2584, 2914, 3026, 3269
PspN4I GGNNCC 8 cut(s) 481, 1146, 1832, 2090, 3650, 3771, 3848, 3968
PspPI GGNCC 9 cut(s) 121, 479, 944, 1145, 1890, 2131, 3179, 3649, 4050
PspPPI RGGWCCY 1 cut(s) 479
PstI CTGCAG 1 cut(s) 1057
PstNI CAGNNNCTG 1 cut(s) 1082
PsuI RGATCY 5 cut(s) 545, 1590, 1749, 3409, 3568
PvuII CAGCTG 1 cut(s) 3719
RseI CAYNNNNRTG 5 cut(s) 1781, 2060, 2504, 2636, 3329
SacI GAGCTC 1 cut(s) 958
Sau96I GGNCC 9 cut(s) 121, 479, 944, 1145, 1890, 2131, 3179, 3649, 4050
ScaI AGTACT 2 cut(s) 2274, 3215
SchI GAGTC 5 cut(s) 927, 2190, 2598, 3513, 4092
ScrFI CCNGG 9 cut(s) 1240, 1322, 1734, 1879, 2103, 2586, 2916, 3028, 3271
SduI GDGCHC 7 cut(s) 334, 958, 1164, 1267, 2902, 3168, 3620
SexAI ACCWGGT 2 cut(s) 2101, 2914
SfaNI GCATC 6 cut(s) 301, 422, 903, 924, 2694, 3013
SfcI CTRYAG 3 cut(s) 1053, 1070, 2109
Sfr274I CTCGAG 2 cut(s) 1986, 4100
SinI GGWCC 8 cut(s) 479, 944, 1145, 1890, 2131, 3179, 3649, 4050
SlaI CTCGAG 2 cut(s) 1986, 4100
SmiMI CAYNNNNRTG 5 cut(s) 1781, 2060, 2504, 2636, 3329
SmlI CTYRAG 8 cut(s) 161, 233, 833, 1745, 1835, 1986, 2135, 4100
SmoI CTYRAG 8 cut(s) 161, 233, 833, 1745, 1835, 1986, 2135, 4100
SnaBI TACGTA 1 cut(s) 564
SseBI AGGCCT 1 cut(s) 3189
SsiI CCGC 2 cut(s) 2294, 4068
SspI AATATT 1 cut(s) 1545
SspMI CTAG 7 cut(s) 129, 678, 726, 1362, 1455, 2841, 3108
SstI GAGCTC 1 cut(s) 958
StuI AGGCCT 1 cut(s) 3189
StyD4I CCNGG 9 cut(s) 1238, 1320, 1732, 1877, 2101, 2584, 2914, 3026, 3269
StyI CCWWGG 4 cut(s) 151, 1893, 2118, 3903
TaiI ACGT 4 cut(s) 344, 368, 566, 1153
TatI WGTACW 7 cut(s) 1842, 2272, 3213, 3333, 3414, 3726, 3861
TauI GCSGC 1 cut(s) 4070
TfiI GAWTC 4 cut(s) 2114, 2786, 2831, 3816
TseFI GTSAC 3 cut(s) 919, 1231, 1297
Tsp45I GTSAC 3 cut(s) 919, 1231, 1297
TspGWI ACGGA 1 cut(s) 3598
Van91I CCANNNNNTGG 2 cut(s) 3014, 3457
VpaK11BI GGWCC 8 cut(s) 479, 944, 1145, 1890, 2131, 3179, 3649, 4050
VspI ATTAAT 1 cut(s) 3228
XagI CCTNNNNNAGG 2 cut(s) 1372, 3396
XapI RAATTY 3 cut(s) 587, 1175, 2069
XbaI TCTAGA 2 cut(s) 725, 1454
XceI RCATGY 4 cut(s) 712, 1823, 1849, 2509
XcmI CCANNNNNNNNNTGG 4 cut(s) 508, 868, 2099, 2163
XhoI CTCGAG 2 cut(s) 1986, 4100
XmnI GAANNNNTTC 1 cut(s) 3971
XspI CTAG 7 cut(s) 129, 678, 726, 1362, 1455, 2841, 3108
ZrmI AGTACT 2 cut(s) 2274, 3215
Zsp2I ATGCAT 1 cut(s) 3458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.