Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 1092, 1955 |
| AccB1I | GGYRCC | 1 cut(s) | 3626 |
| AccB7I | CCANNNNNTGG | 3 cut(s) | 491, 2981, 3424 |
| AccI | GTMKAC | 1 cut(s) | 3780 |
| AccII | CGCG | 2 cut(s) | 25, 1150 |
| AccIII | TCCGGA | 1 cut(s) | 1195 |
| AciI | CCGC | 8 cut(s) | 241, 556, 788, 1148, 1971, 2492, 3261, 3699 |
| AclI | AACGTT | 2 cut(s) | 2687, 3500 |
| AcoI | YGGCCR | 2 cut(s) | 519, 3648 |
| AcsI | RAATTY | 7 cut(s) | 393, 544, 587, 1015, 2069, 2148, 2302 |
| AcuI | CTGAAG | 5 cut(s) | 51, 800, 2156, 2546, 3240 |
| AcyI | GRCGYC | 1 cut(s) | 1874 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 491, 1908, 2230, 2981, 3365, 3424, 3955, 3978, 4026 |
| AflIII | ACRYGT | 3 cut(s) | 88, 363, 708 |
| AgeI | ACCGGT | 2 cut(s) | 946, 2891 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 851 |
| AjiI | CACGTC | 1 cut(s) | 1268 |
| AjnI | CCWGG | 5 cut(s) | 1715, 2551, 2881, 3236, 4025 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 3951, 3983 |
| Alw21I | GWGCWC | 4 cut(s) | 334, 1164, 2869, 2940 |
| Alw26I | GTCTC | 9 cut(s) | 653, 800, 1066, 1266, 1689, 2032, 2083, 2145, 4056 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1082 |
| Ama87I | CYCGRG | 4 cut(s) | 1163, 1986, 2255, 3955 |
| Aor13HI | TCCGGA | 1 cut(s) | 1195 |
| AoxI | GGCC | 5 cut(s) | 519, 1144, 2549, 2983, 3648 |
| ApaI | GGGCCC | 1 cut(s) | 1148 |
| ApoI | RAATTY | 7 cut(s) | 393, 544, 587, 1015, 2069, 2148, 2302 |
| AsiGI | ACCGGT | 2 cut(s) | 946, 2891 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 3694, 3950 |
| AspLEI | GCGC | 2 cut(s) | 64, 2913 |
| AspS9I | GGNCC | 6 cut(s) | 479, 944, 1144, 1145, 3146, 4029 |
| AsuC2I | CCSGG | 2 cut(s) | 553, 3230 |
| AsuHPI | GGTGA | 6 cut(s) | 128, 202, 1243, 3140, 3406, 3838 |
| AsuII | TTCGAA | 1 cut(s) | 391 |
| AvaI | CYCGRG | 4 cut(s) | 1163, 1986, 2255, 3955 |
| AvaII | GGWCC | 4 cut(s) | 479, 944, 3146, 4029 |
| AxyI | CCTNAGG | 1 cut(s) | 2790 |
| BaeGI | GKGCMC | 2 cut(s) | 1148, 1267 |
| BamHI | GGATCC | 2 cut(s) | 1740, 1937 |
| BanI | GGYRCC | 1 cut(s) | 3626 |
| BanII | GRGCYC | 3 cut(s) | 1148, 2869, 2940 |
| BarI | GAAGNNNNNNTAC | 6 cut(s) | 576, 608, 1510, 1542, 3818, 3850 |
| BbsI | GAAGAC | 3 cut(s) | 12, 39, 532 |
| Bbv12I | GWGCWC | 4 cut(s) | 334, 1164, 2869, 2940 |
| BceAI | ACGGC | 2 cut(s) | 2017, 3635 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1340, 1374, 2934, 2968 |
| BciT130I | CCWGG | 5 cut(s) | 1717, 2553, 2883, 3238, 4027 |
| BciVI | GTATCC | 2 cut(s) | 2699, 4118 |
| BclI | TGATCA | 1 cut(s) | 3174 |
| BcnI | CCSGG | 2 cut(s) | 553, 3230 |
| BcoDI | GTCTC | 9 cut(s) | 653, 800, 1066, 1266, 1689, 2032, 2083, 2145, 4056 |
| BfaI | CTAG | 4 cut(s) | 129, 678, 794, 2994 |
| BfmI | CTRYAG | 4 cut(s) | 1053, 2109, 2237, 3774 |
| BfuI | GTATCC | 2 cut(s) | 2699, 4118 |
| BglII | AGATCT | 3 cut(s) | 2349, 3376, 3565 |
| BmcAI | AGTACT | 1 cut(s) | 1259 |
| Bme1390I | CCNGG | 7 cut(s) | 553, 1717, 2553, 2883, 3230, 3238, 4027 |
| Bme18I | GGWCC | 4 cut(s) | 479, 944, 3146, 4029 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 851 |
| BmeT110I | CYCGRG | 4 cut(s) | 1163, 1986, 2255, 3955 |
| BmgBI | CACGTC | 1 cut(s) | 1268 |
| BmgT120I | GGNCC | 6 cut(s) | 479, 944, 1144, 1145, 3146, 4029 |
| BmrFI | CCNGG | 7 cut(s) | 553, 1717, 2553, 2883, 3230, 3238, 4027 |
| BmrI | ACTGGG | 2 cut(s) | 1270, 2032 |
| BmuI | ACTGGG | 2 cut(s) | 1270, 2032 |
| BpiI | GAAGAC | 3 cut(s) | 12, 39, 532 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1686, 1718 |
| BpmI | CTGGAG | 3 cut(s) | 1055, 3266, 4100 |
| Bpu14I | TTCGAA | 1 cut(s) | 391 |
| BpuEI | CTTGAG | 3 cut(s) | 218, 818, 1730 |
| BpuMI | CCSGG | 2 cut(s) | 553, 3230 |
| Bsa29I | ATCGAT | 2 cut(s) | 1541, 2954 |
| BsaAI | YACGTR | 1 cut(s) | 89 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1754, 2819 |
| BsaHI | GRCGYC | 1 cut(s) | 1874 |
| BsaI | GGTCTC | 4 cut(s) | 800, 1066, 1689, 2032 |
| BsaJI | CCNNGG | 6 cut(s) | 1716, 1902, 2118, 3363, 3538, 3882 |
| BsaWI | WCCGGW | 4 cut(s) | 282, 946, 1195, 2891 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 2856, 2886, 3762, 3792, 4080, 4110 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 491, 1908, 2230, 2981, 3365, 3424, 3955, 3978, 4026 |
| Bse118I | RCCGGY | 5 cut(s) | 946, 980, 2891, 3143, 3640 |
| Bse1I | ACTGG | 6 cut(s) | 1265, 1321, 2027, 2635, 3452, 4083 |
| Bse21I | CCTNAGG | 1 cut(s) | 2790 |
| Bse3DI | GCAATG | 7 cut(s) | 202, 3160, 3342, 3504, 3585, 3906, 4175 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1754, 2819 |
| BseAI | TCCGGA | 1 cut(s) | 1195 |
| BseBI | CCWGG | 5 cut(s) | 1717, 2553, 2883, 3238, 4027 |
| BseCI | ATCGAT | 2 cut(s) | 1541, 2954 |
| BseDI | CCNNGG | 6 cut(s) | 1716, 1902, 2118, 3363, 3538, 3882 |
| BseGI | GGATG | 9 cut(s) | 585, 973, 1032, 1588, 1918, 2418, 2614, 3063, 3246 |
| BseJI | GATNNNNATC | 2 cut(s) | 1754, 2819 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 491, 1908, 2230, 2981, 3365, 3424, 3955, 3978, 4026 |
| BseMI | GCAATG | 7 cut(s) | 202, 3160, 3342, 3504, 3585, 3906, 4175 |
| BseMII | CTCAG | 7 cut(s) | 611, 1587, 2279, 2286, 2781, 2895, 3645 |
| BseNI | ACTGG | 6 cut(s) | 1265, 1321, 2027, 2635, 3452, 4083 |
| BseRI | GAGGAG | 2 cut(s) | 1058, 2210 |
| BseSI | GKGCMC | 2 cut(s) | 1148, 1267 |
| BsgI | GTGCAG | 2 cut(s) | 3498, 3656 |
| Bsh1236I | CGCG | 2 cut(s) | 25, 1150 |
| BshFI | GGCC | 5 cut(s) | 521, 1146, 2551, 2985, 3650 |
| BshNI | GGYRCC | 1 cut(s) | 3626 |
| BshTI | ACCGGT | 2 cut(s) | 946, 2891 |
| BshVI | ATCGAT | 2 cut(s) | 1541, 2954 |
| BsiHKAI | GWGCWC | 4 cut(s) | 334, 1164, 2869, 2940 |
| BsiHKCI | CYCGRG | 4 cut(s) | 1163, 1986, 2255, 3955 |
| BslFI | GGGAC | 2 cut(s) | 135, 465 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 491, 1908, 2230, 2981, 3365, 3424, 3955, 3978, 4026 |
| BsmAI | GTCTC | 9 cut(s) | 653, 800, 1066, 1266, 1689, 2032, 2083, 2145, 4056 |
| BsmBI | CGTCTC | 1 cut(s) | 4056 |
| BsmFI | GGGAC | 2 cut(s) | 135, 465 |
| BsmI | GAATGC | 1 cut(s) | 381 |
| BsnI | GGCC | 5 cut(s) | 521, 1146, 2551, 2985, 3650 |
| Bso31I | GGTCTC | 4 cut(s) | 800, 1066, 1689, 2032 |
| BsoBI | CYCGRG | 4 cut(s) | 1163, 1986, 2255, 3955 |
| Bsp119I | TTCGAA | 1 cut(s) | 391 |
| Bsp120I | GGGCCC | 1 cut(s) | 1144 |
| Bsp1286I | GDGCHC | 6 cut(s) | 334, 1148, 1164, 1267, 2869, 2940 |
| Bsp13I | TCCGGA | 1 cut(s) | 1195 |
| Bsp19I | CCATGG | 1 cut(s) | 3882 |
| Bsp68I | TCGCGA | 1 cut(s) | 25 |
| BspACI | CCGC | 8 cut(s) | 241, 556, 788, 1148, 1971, 2492, 3261, 3699 |
| BspANI | GGCC | 5 cut(s) | 521, 1146, 2551, 2985, 3650 |
| BspCNI | CTCAG | 7 cut(s) | 610, 1586, 2278, 2287, 2782, 2896, 3644 |
| BspDI | ATCGAT | 2 cut(s) | 1541, 2954 |
| BspEI | TCCGGA | 1 cut(s) | 1195 |
| BspFNI | CGCG | 2 cut(s) | 25, 1150 |
| BspHI | TCATGA | 1 cut(s) | 1470 |
| BspMAI | CTGCAG | 2 cut(s) | 1057, 2241 |
| BspT104I | TTCGAA | 1 cut(s) | 391 |
| BspT107I | GGYRCC | 1 cut(s) | 3626 |
| BspTNI | GGTCTC | 4 cut(s) | 800, 1066, 1689, 2032 |
| BsrDI | GCAATG | 7 cut(s) | 202, 3160, 3342, 3504, 3585, 3906, 4175 |
| BsrFI | RCCGGY | 5 cut(s) | 946, 980, 2891, 3143, 3640 |
| BsrI | ACTGG | 6 cut(s) | 1265, 1321, 2027, 2635, 3452, 4083 |
| BssAI | RCCGGY | 5 cut(s) | 946, 980, 2891, 3143, 3640 |
| BssECI | CCNNGG | 6 cut(s) | 1716, 1902, 2118, 3363, 3538, 3882 |
| BssNAI | GTATAC | 1 cut(s) | 3781 |
| BssNI | GRCGYC | 1 cut(s) | 1874 |
| BssT1I | CCWWGG | 5 cut(s) | 1902, 2118, 3363, 3538, 3882 |
| Bst1107I | GTATAC | 1 cut(s) | 3781 |
| Bst2UI | CCWGG | 5 cut(s) | 1717, 2553, 2883, 3238, 4027 |
| Bst6I | CTCTTC | 8 cut(s) | 91, 724, 965, 1469, 1709, 2359, 3575, 3956 |
| BstACI | GRCGYC | 1 cut(s) | 1874 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 376 |
| BstBAI | YACGTR | 1 cut(s) | 89 |
| BstBI | TTCGAA | 1 cut(s) | 391 |
| BstC8I | GCNNGC | 9 cut(s) | 27, 1077, 1148, 1359, 1447, 1927, 2590, 2695, 3430 |
| BstDSI | CCRYGG | 1 cut(s) | 3882 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1906 |
| BstF5I | GGATG | 9 cut(s) | 585, 973, 1032, 1588, 1918, 2418, 2614, 3063, 3246 |
| BstFNI | CGCG | 2 cut(s) | 25, 1150 |
| BstHHI | GCGC | 2 cut(s) | 64, 2913 |
| BstMAI | GTCTC | 9 cut(s) | 653, 800, 1066, 1266, 1689, 2032, 2083, 2145, 4056 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 70, 376, 674, 2917, 2999, 3162, 3269 |
| BstNI | CCWGG | 5 cut(s) | 1717, 2553, 2883, 3238, 4027 |
| BstNSI | RCATGY | 3 cut(s) | 367, 712, 1449 |
| BstSCI | CCNGG | 7 cut(s) | 551, 1715, 2551, 2881, 3228, 3236, 4025 |
| BstSFI | CTRYAG | 4 cut(s) | 1053, 2109, 2237, 3774 |
| BstSLI | GKGCMC | 2 cut(s) | 1148, 1267 |
| BstUI | CGCG | 2 cut(s) | 25, 1150 |
| BstV2I | GAAGAC | 3 cut(s) | 12, 39, 532 |
| BstX2I | RGATCY | 6 cut(s) | 1740, 1749, 1937, 2349, 3376, 3565 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 2007, 2605 |
| BstYI | RGATCY | 6 cut(s) | 1740, 1749, 1937, 2349, 3376, 3565 |
| BstZ17I | GTATAC | 1 cut(s) | 3781 |
| Bsu15I | ATCGAT | 2 cut(s) | 1541, 2954 |
| Bsu36I | CCTNAGG | 1 cut(s) | 2790 |
| BsuI | GTATCC | 2 cut(s) | 2699, 4118 |
| BsuRI | GGCC | 5 cut(s) | 521, 1146, 2551, 2985, 3650 |
| BsuTUI | ATCGAT | 2 cut(s) | 1541, 2954 |
| BtgI | CCRYGG | 1 cut(s) | 3882 |
| BtgZI | GCGATG | 1 cut(s) | 1643 |
| BtrI | CACGTC | 1 cut(s) | 1268 |
| BtsCI | GGATG | 9 cut(s) | 585, 973, 1032, 1588, 1918, 2418, 2614, 3063, 3246 |
| BtsI | GCAGTG | 3 cut(s) | 332, 1062, 1946 |
| BtuMI | TCGCGA | 1 cut(s) | 25 |
| Cac8I | GCNNGC | 9 cut(s) | 27, 1077, 1148, 1359, 1447, 1927, 2590, 2695, 3430 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1082 |
| CciI | TCATGA | 1 cut(s) | 1470 |
| CfoI | GCGC | 2 cut(s) | 64, 2913 |
| Cfr10I | RCCGGY | 5 cut(s) | 946, 980, 2891, 3143, 3640 |
| Cfr13I | GGNCC | 6 cut(s) | 479, 944, 1144, 1145, 3146, 4029 |
| ClaI | ATCGAT | 2 cut(s) | 1541, 2954 |
| CseI | GACGC | 2 cut(s) | 281, 1863 |
| CsiI | ACCWGGT | 1 cut(s) | 2881 |
| CspAI | ACCGGT | 2 cut(s) | 946, 2891 |
| DraI | TTTAAA | 1 cut(s) | 543 |
| DriI | GACNNNNNGTC | 1 cut(s) | 851 |
| EaeI | YGGCCR | 2 cut(s) | 519, 3648 |
| Eam1104I | CTCTTC | 8 cut(s) | 91, 724, 965, 1469, 1709, 2359, 3575, 3956 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 851 |
| EarI | CTCTTC | 8 cut(s) | 91, 724, 965, 1469, 1709, 2359, 3575, 3956 |
| EciI | GGCGGA | 2 cut(s) | 571, 1986 |
| Ecl136II | GAGCTC | 2 cut(s) | 2867, 2938 |
| Eco130I | CCWWGG | 5 cut(s) | 1902, 2118, 3363, 3538, 3882 |
| Eco24I | GRGCYC | 3 cut(s) | 1148, 2869, 2940 |
| Eco31I | GGTCTC | 4 cut(s) | 800, 1066, 1689, 2032 |
| Eco32I | GATATC | 1 cut(s) | 2725 |
| Eco47I | GGWCC | 4 cut(s) | 479, 944, 3146, 4029 |
| Eco53kI | GAGCTC | 2 cut(s) | 2867, 2938 |
| Eco57I | CTGAAG | 5 cut(s) | 51, 800, 2156, 2546, 3240 |
| Eco81I | CCTNAGG | 1 cut(s) | 2790 |
| Eco88I | CYCGRG | 4 cut(s) | 1163, 1986, 2255, 3955 |
| EcoICRI | GAGCTC | 2 cut(s) | 2867, 2938 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1906 |
| EcoO109I | RGGNCCY | 2 cut(s) | 479, 1144 |
| EcoRII | CCWGG | 5 cut(s) | 1715, 2551, 2881, 3236, 4025 |
| EcoRV | GATATC | 1 cut(s) | 2725 |
| EcoT14I | CCWWGG | 5 cut(s) | 1902, 2118, 3363, 3538, 3882 |
| EcoT22I | ATGCAT | 2 cut(s) | 1566, 3301 |
| EcoT38I | GRGCYC | 3 cut(s) | 1148, 2869, 2940 |
| ErhI | CCWWGG | 5 cut(s) | 1902, 2118, 3363, 3538, 3882 |
| Esp3I | CGTCTC | 1 cut(s) | 4056 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 2527, 2559 |
| FaqI | GGGAC | 2 cut(s) | 135, 465 |
| FauI | CCCGC | 1 cut(s) | 1155 |
| FauNDI | CATATG | 2 cut(s) | 1190, 1819 |
| FbaI | TGATCA | 1 cut(s) | 3174 |
| FblI | GTMKAC | 1 cut(s) | 3780 |
| FokI | GGATG | 9 cut(s) | 592, 980, 1019, 1595, 1925, 2425, 2621, 3050, 3253 |
| FriOI | GRGCYC | 3 cut(s) | 1148, 2869, 2940 |
| FspBI | CTAG | 4 cut(s) | 129, 678, 794, 2994 |
| GlaI | GCGC | 2 cut(s) | 63, 2912 |
| GsuI | CTGGAG | 3 cut(s) | 1055, 3266, 4100 |
| HaeIII | GGCC | 5 cut(s) | 521, 1146, 2551, 2985, 3650 |
| HgaI | GACGC | 2 cut(s) | 281, 1863 |
| HhaI | GCGC | 2 cut(s) | 64, 2913 |
| Hin1I | GRCGYC | 1 cut(s) | 1874 |
| Hin6I | GCGC | 2 cut(s) | 62, 2911 |
| HinP1I | GCGC | 2 cut(s) | 62, 2911 |
| HincII | GTYRAC | 2 cut(s) | 1423, 3286 |
| HindII | GTYRAC | 2 cut(s) | 1423, 3286 |
| HindIII | AAGCTT | 1 cut(s) | 1314 |
| HpaI | GTTAAC | 2 cut(s) | 1423, 3286 |
| HphI | GGTGA | 6 cut(s) | 128, 202, 1243, 3140, 3406, 3838 |
| Hpy166II | GTNNAC | 8 cut(s) | 86, 1423, 1594, 2636, 3037, 3286, 3495, 3781 |
| Hpy8I | GTNNAC | 8 cut(s) | 86, 1423, 1594, 2636, 3037, 3286, 3495, 3781 |
| Hpy99I | CGWCG | 2 cut(s) | 1272, 1355 |
| HpyCH4IV | ACGT | 8 cut(s) | 88, 341, 503, 1267, 1494, 2687, 3500, 4065 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 70, 376, 674, 2917, 2999, 3162, 3269 |
| HpySE526I | ACGT | 8 cut(s) | 88, 341, 503, 1267, 1494, 2687, 3500, 4065 |
| Hsp92I | GRCGYC | 1 cut(s) | 1874 |
| HspAI | GCGC | 2 cut(s) | 62, 2911 |
| Kpn2I | TCCGGA | 1 cut(s) | 1195 |
| Ksp22I | TGATCA | 1 cut(s) | 3174 |
| KspAI | GTTAAC | 2 cut(s) | 1423, 3286 |
| LmnI | GCTCC | 7 cut(s) | 1601, 2781, 2845, 2864, 3161, 3170, 3770 |
| MabI | ACCWGGT | 1 cut(s) | 2881 |
| MaeI | CTAG | 4 cut(s) | 129, 678, 794, 2994 |
| MaeII | ACGT | 8 cut(s) | 88, 341, 503, 1267, 1494, 2687, 3500, 4065 |
| MfeI | CAATTG | 2 cut(s) | 273, 1332 |
| MflI | RGATCY | 6 cut(s) | 1740, 1749, 1937, 2349, 3376, 3565 |
| MhlI | GDGCHC | 6 cut(s) | 334, 1148, 1164, 1267, 2869, 2940 |
| MlyI | GAGTC | 6 cut(s) | 460, 927, 1565, 2565, 2586, 4091 |
| MmeI | TCCRAC | 6 cut(s) | 73, 141, 159, 673, 922, 2066 |
| Mph1103I | ATGCAT | 2 cut(s) | 1566, 3301 |
| MroI | TCCGGA | 1 cut(s) | 1195 |
| MroXI | GAANNNNTTC | 2 cut(s) | 3694, 3950 |
| MslI | CAYNNNNRTG | 3 cut(s) | 713, 2471, 2603 |
| MspA1I | CMGCKG | 2 cut(s) | 788, 3686 |
| MspR9I | CCNGG | 7 cut(s) | 553, 1717, 2553, 2883, 3230, 3238, 4027 |
| MunI | CAATTG | 2 cut(s) | 273, 1332 |
| Mva1269I | GAATGC | 1 cut(s) | 381 |
| MvaI | CCWGG | 5 cut(s) | 1717, 2553, 2883, 3238, 4027 |
| MvnI | CGCG | 2 cut(s) | 25, 1150 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 70, 376, 674, 2917, 2999, 3162, 3269 |
| NciI | CCSGG | 2 cut(s) | 553, 3230 |
| NcoI | CCATGG | 1 cut(s) | 3882 |
| NdeI | CATATG | 2 cut(s) | 1190, 1819 |
| NmeAIII | GCCGAG | 2 cut(s) | 653, 1326 |
| NmuCI | GTSAC | 3 cut(s) | 919, 1231, 2740 |
| NruI | TCGCGA | 1 cut(s) | 25 |
| NsiI | ATGCAT | 2 cut(s) | 1566, 3301 |
| NspI | RCATGY | 3 cut(s) | 367, 712, 1449 |
| NspV | TTCGAA | 1 cut(s) | 391 |
| PaeI | GCATGC | 1 cut(s) | 1449 |
| PaeR7I | CTCGAG | 2 cut(s) | 1163, 1986 |
| PagI | TCATGA | 1 cut(s) | 1470 |
| PciI | ACATGT | 2 cut(s) | 363, 708 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 3400, 3983 |
| PctI | GAATGC | 1 cut(s) | 381 |
| PdmI | GAANNNNTTC | 2 cut(s) | 3694, 3950 |
| PflMI | CCANNNNNTGG | 3 cut(s) | 491, 2981, 3424 |
| PinAI | ACCGGT | 2 cut(s) | 946, 2891 |
| PleI | GAGTC | 6 cut(s) | 460, 926, 1565, 2564, 2585, 4090 |
| PpsI | GAGTC | 6 cut(s) | 460, 926, 1565, 2564, 2585, 4090 |
| Ppu21I | YACGTR | 1 cut(s) | 89 |
| PpuMI | RGGWCCY | 1 cut(s) | 479 |
| PscI | ACATGT | 2 cut(s) | 363, 708 |
| PsiI | TTATAA | 2 cut(s) | 1092, 1955 |
| Psp124BI | GAGCTC | 2 cut(s) | 2869, 2940 |
| Psp1406I | AACGTT | 2 cut(s) | 2687, 3500 |
| Psp5II | RGGWCCY | 1 cut(s) | 479 |
| Psp6I | CCWGG | 5 cut(s) | 1715, 2551, 2881, 3236, 4025 |
| PspGI | CCWGG | 5 cut(s) | 1715, 2551, 2881, 3236, 4025 |
| PspOMI | GGGCCC | 1 cut(s) | 1144 |
| PspPI | GGNCC | 6 cut(s) | 479, 944, 1144, 1145, 3146, 4029 |
| PspPPI | RGGWCCY | 1 cut(s) | 479 |
| PspXI | VCTCGAGB | 1 cut(s) | 1163 |
| PstI | CTGCAG | 2 cut(s) | 1057, 2241 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1082 |
| PsuI | RGATCY | 6 cut(s) | 1740, 1749, 1937, 2349, 3376, 3565 |
| PvuII | CAGCTG | 1 cut(s) | 3686 |
| RruI | TCGCGA | 1 cut(s) | 25 |
| RseI | CAYNNNNRTG | 3 cut(s) | 713, 2471, 2603 |
| SacI | GAGCTC | 2 cut(s) | 2869, 2940 |
| Sau96I | GGNCC | 6 cut(s) | 479, 944, 1144, 1145, 3146, 4029 |
| ScaI | AGTACT | 1 cut(s) | 1259 |
| SchI | GAGTC | 6 cut(s) | 460, 927, 1565, 2565, 2586, 4091 |
| ScrFI | CCNGG | 7 cut(s) | 553, 1717, 2553, 2883, 3230, 3238, 4027 |
| SduI | GDGCHC | 6 cut(s) | 334, 1148, 1164, 1267, 2869, 2940 |
| SexAI | ACCWGGT | 1 cut(s) | 2881 |
| SfcI | CTRYAG | 4 cut(s) | 1053, 2109, 2237, 3774 |
| Sfr274I | CTCGAG | 2 cut(s) | 1163, 1986 |
| SfuI | TTCGAA | 1 cut(s) | 391 |
| SinI | GGWCC | 4 cut(s) | 479, 944, 3146, 4029 |
| SlaI | CTCGAG | 2 cut(s) | 1163, 1986 |
| SmiI | ATTTAAAT | 1 cut(s) | 543 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 713, 2471, 2603 |
| SmlI | CTYRAG | 5 cut(s) | 233, 833, 1163, 1745, 1986 |
| SmoI | CTYRAG | 5 cut(s) | 233, 833, 1163, 1745, 1986 |
| SphI | GCATGC | 1 cut(s) | 1449 |
| SsiI | CCGC | 8 cut(s) | 241, 556, 788, 1148, 1971, 2492, 3261, 3699 |
| SspMI | CTAG | 4 cut(s) | 129, 678, 794, 2994 |
| SstI | GAGCTC | 2 cut(s) | 2869, 2940 |
| StyD4I | CCNGG | 7 cut(s) | 551, 1715, 2551, 2881, 3228, 3236, 4025 |
| StyI | CCWWGG | 5 cut(s) | 1902, 2118, 3363, 3538, 3882 |
| SwaI | ATTTAAAT | 1 cut(s) | 543 |
| TaiI | ACGT | 8 cut(s) | 91, 344, 506, 1270, 1497, 2690, 3503, 4068 |
| TatI | WGTACW | 5 cut(s) | 1257, 1842, 1849, 3840, 3855 |
| TauI | GCSGC | 2 cut(s) | 791, 3263 |
| TseFI | GTSAC | 3 cut(s) | 919, 1231, 2740 |
| Tsp45I | GTSAC | 3 cut(s) | 919, 1231, 2740 |
| TspGWI | ACGGA | 3 cut(s) | 585, 1653, 3783 |
| Van91I | CCANNNNNTGG | 3 cut(s) | 491, 2981, 3424 |
| VpaK11BI | GGWCC | 4 cut(s) | 479, 944, 3146, 4029 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1906 |
| XapI | RAATTY | 7 cut(s) | 393, 544, 587, 1015, 2069, 2148, 2302 |
| XbaI | TCTAGA | 1 cut(s) | 793 |
| XceI | RCATGY | 3 cut(s) | 367, 712, 1449 |
| XcmI | CCANNNNNNNNNTGG | 4 cut(s) | 508, 1043, 1874, 2287 |
| XhoI | CTCGAG | 2 cut(s) | 1163, 1986 |
| XmiI | GTMKAC | 1 cut(s) | 3780 |
| XmnI | GAANNNNTTC | 2 cut(s) | 3694, 3950 |
| XspI | CTAG | 4 cut(s) | 129, 678, 794, 2994 |
| ZrmI | AGTACT | 1 cut(s) | 1259 |
| Zsp2I | ATGCAT | 2 cut(s) | 1566, 3301 |