Prupe.1G471300_v2.0.a1
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000493
Physical Location & Seq
Reverse (-)
39219805 .. 39226716
6912 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G471300.2

Sequence Viewer

Length: 3777 bp
ATGACACTGCTTTTAGGCCCCCCGGGCTCTGGAAAGACCACATTACTATTGGCATTGGCTGGAAAACTTGGTAAAGATCTAAAACTTTCTGGGAGAGTTACATACAACGGACACGGGATGGAAGAGTTTATACCGGAGAGGACCTCAGCTTATATTAGCCAGCATGATCTCCACATACCAGAATTGACAGTCAGAGAAACGCTGGCTTTTTCTGCAAGATGTCAAGGGGTTGGACCGCGTTATGAAATGTTGGTAGAATTATCAAGAAGAGAGAAGGCTGCAAATATTAAGCCAGATCCTGATCTTGATGTTTACATGAAGGCAGCAGCACTGGAAGGACAGGAAACCAATGTGGTTACAGATTACATAATCAAGGTTTTGGGACTAGAAGTTTGTGCTGACACCATGGTAGGGGATCAAATGAGACGAGGTATATCTGGGGGCCAAAAAAAGCGACTCACCACAGGGGAGATGCTGGTTGGACCAGAAAAAGCACTTTTTATGGATGAGATATCAACTGGGTTGGACAGTTCAACAACATTTCACATTGTGAATTCACTCAGACAATCCATCCACATTCTCAATGGAACTGCCCTAATCTCTCTCCTGCAACCAGCACCAGAAACTTATGAACTCTTTGATGATATAATTCTCCTCTCAGATGGGCGCATTGTATATCAAGGTCCGCGCGAGAATGTGCTCGAGTTCTTTGAGCACAGGGGCTTCAAATGTCCAGAGAGGAAAGGAGTTGCTGATTTCCTACAAGAAGTGACATCAAGGAAAGATCAAGAGCAATACTGGGCTGATAAAGATAAGCCTTACAGCTTTGTAACTTCAAATGAATTTTCTGAAGCAATGCAATCATTTCGCATTGGTAGAGAACTTGGGGATGAGCTTGCCACACCATTTGACAAGTCTAAAGGGAACCCAGCAGCTTTAACAACTAACAAGTATGGAGTTAGCAAGAAGGAACTTTACAAAGCTTGTATGTCAAGACAAGTTTTGCTTATGAAGAGGAATTCATTTGTCTACATTTTCAAAATGACCCAGTTCATTATAATGGCTTTTACAACAATGACACTATTCCTACGAACTGAGATGCATCGACGTACCGTGGAAGATGGTGGGATTTACATGGGATCTTTGTTTTACACAATGATGATAATTATGTTCACTGGATTCTCAGAACTTGCTATGACTGTCATGAGACTTCCTGTGTTTTTCAAGCAAAGGGACCTTCTCTTCTTTCCTGCTTGGGCATACTCTTTGCCAACATGTCTGATAAGAATCCCTCTGACCTTTGTGGAAGCTTTCATTTGGGTGGCCATGACTTACTATGTTATAGGTTATGATCCGAGCATTGAAAGGTTCTTCAAACAGTTCATTTTACTACTGTGCATTAGTCAGATGGCAAATGGTCTGTTCCGATTGTTAGCGGCGGTAGGCAGGAGCCCAGTTGTTGCAAACACATTTGGGTCTGCTGCTTTACTTGTCCTTTTTGTTCTTGGTGGATTTATCTTATCACGAGAAAGTATGCAAGAATGGTTGTTGTGGGGATATTGGTTCTCACCGCTGACATATGGAATGAATGCATTAGCTGTCAATGAATTTCTTGGAAAAAGTTGGAGACATGTTCCTGCCAACTCAACAGAACCATTAGGAGTTATGGTCTTGAAGTCTCGAGGGGTATTACCAGAAGCACACTGGTACTGGATTGGAGTAGTAGCTTCACTTGGATTTGTTCTTCTGTTCAACTTCCTTTTTACTTTCGCACTCCAGTATCTTGATCCCATTGACAAGCCTCAGGCAGTAATGTCCAAAGAGGCCTTGGAAGAGAAACTTGCCAAGAAAAATGGAGAGACCGTTGAGCTACCGTCAATGGGAAAGAGCTCTGTTGGAGTAGGGAATGAAAGTTTGGAGAGTGTCTCTACCAATCATACCAGGCGGCGAGGAATGGTTCTTCCTTTTGAAACCCTCTCAATGACGTTCAATGAAATCAGATATGCAGTTGACATGCCACAGGAAATGAAAGCTGAAGGCATAACTGAGGATCGGCTAGAACTTCTGAAGGGTGTGAGTGGTGCTTTTAGGCCTGGTGTCCTAACAGCTCTAATGGGGGTTAGTGGTGCAGGGAAGACTACTTTAATGGATGTCTTGGCAGGACGGAAAACAGGTGGATATATCGAAGGAAGCATCATTGTATCTGGATATCCAAAAAATCAAGAAACATTTGCTCGCATATCAGGATATTGTGAGCAAACTGATATACACTCTCCTCATGTCACAGTCTATGAGTCTTTGGTGTATTCTGCATGGCTCCGATTGCCCCCAGGGGTTGATTCCCCAACCAGAAAGATGTTCATTGAGGAAGTCATGGAGCTTGTTGAACTGACCTCAATAAGGGAAGCACTTGTTGGATTGCCTGGAGTGAATGGTCTTTCAACTGAGCAGCGCAAAAGGCTAACGATTGCAGTAGAACTTGTTGCCAACCCATCCATAATATTTATGGATGAGCCGACCTCTGGCCTTGATGCAAGGGCGGCAGCAATTGTAATGAGAACAGTGAGGAATACAGTGGACACCGGGCGAACTGTAGTCTGCACCATCCACCAGCCAAGCATTGATATATTTGATGCTTTTGATGAGCTGTTTCTTCTGAAACGGGGAGGAGAAGAAATATATGTTGGTCCTTTAGGCCACCAGTCTTCCCAGTTGATCAATTACTTTGAGGGAATTAATGGAGTTTCTAAACTAAGAGATGGCTACAATCCTGCAACTTGGATGTTGGAGGTTACTTCAGCAGGACAAGAAGCGGCTCTTGGGGTTAATTTCACTGACATATATAAGAATTCAGAAGTATACAGGAGAAACAAGGCCTTGATCAAGGAACTAAGTACTCCTCCACCAAATTCAAGAGACTTGTTCTTCCCTACACAGTACTCACAGTCCTTCTTTACCCAGTGCATAGCTTGCCTATGGAAACAGCATTGGTCTTATTGGCGAAACCCGTCATACAGTGCAGTGAGACTTTTGTACACTGCTGTGATGGCTCTAGTATTTGGGATAATATTCTGGGATCTTGGCTCCAAAAGGCATAGGCAACAAGATCTTTTTAATGCAATGGGATCTATGTACTCTGCTGTTCTCTTCATTGGAATACAAAATGCCTCATCAGTGCAGCCAGTTGTAGGCATTGAGAGGGTAGTCTTTTACAGAGAAAGGGCTGCTGGAATGTACTCAGCTTTTCCATATGCCTTGGGACAGGTTCTGATTGAGCTTCCATACACTTCGATCCAAACAATCATCTATGGAGTTATAGTATACAGCATGATCGGATTTGAATGGACGGTCAGCAAGTTCTTGTGGCACATCTTCTTCATGTACTTCACCTTCTTATACTACATCTTGTATGGTATGATGATTGTGGGCATTACTCCAAACACAACCATTGCCGCTGTCGCTTCCTCAGCCTTCTACCCATTATGGAACGTCTTTTCAGGATTTATCATTCCGAAAACAAGAATTCCAATATGGTGGAGATGGTTCTACTGGGTGTGTCCAGTGTCTTGGACCTTGTATGGATTGTTTACTTCACAGTTTGGAGGCATCAAGGACACACTTGATTCGGGTGAAACTGTGGACGATTTTATAAGGGCTTATTTTGGATACACAAAAGATTTTTTAGGTGTTGTTGCAATTGTGCATGTTGGAATTTCAGGGCTCTTTGGTTTCATCTTTGCATTTTCAATCAAAGTATTTAACTTCCAAAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1259

Amino Acids

141.77

Weight (kDa)

7.02

Isoelectric Point (pI)

37.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000493)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29820 FvH4_2g29820 FvH4_2g29822 FvH4_2g29824 FvH4_2g29824 FvH4_2g29824 FvH4_2g29840 FvH4_2g29840 FvH4_2g29840 FvH4_5g00550 FvH4_5g00550
malus_domestica MD02G1032700.v1.1 MD08G1140900.v1.1 MD08G1141400.v1.1 MD15G1117800.v1.1 MD15G1117900.v1.1
prunus_persica Prupe.1G471200_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471300_v2.0.a1 Prupe.1G471400_v2.0.a1 Prupe.7G242900_v2.0.a1
pyrus_communis pycom02g02690 pycom08g11890
rosa_chinensis RchiOBHm_Chr2g0111741 RchiOBHm_Chr2g0145991 RchiOBHm_Chr6g0298851 RchiOBHm_Chr6g0298871 RchiOBHm_Chr6g0298891 RchiOBHm_Chr6g0298901 RchiOBHm_Chr6g0298911 RchiOBHm_Chr7g0201801 RchiOBHm_Chr7g0211531 RchiOBHm_Chr7g0214601
rosa_laevigata RLG00000002931 RLG00000003000 RLG00000003647 RLG00000011476 RLG00000011479
rosa_multiflora Rmu_sc0001030.1_g000012 Rmu_sc0001548.1_g000041 Rmu_ssc0000397.1_g000033 Rmu_ssc0000397.1_g000036 Rmu_ssc0000397.1_g000037 Rmu_ssc0000397.1_g000039 Rmu_ssc0000397.1_g000048
rosa_roxburghii Rroxscaffold_1G00003830 Rroxscaffold_3G00247810 Rroxscaffold_3G00255060 Rroxscaffold_7G00169220 Rroxscaffold_7G00169230 Rroxscaffold_7G00169280
rosa_rugosa Rorug02G0223600 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283100 Rorug06G0283200 Rorug06G0283300 Rorug06G0283400 Rorug06G0283500 Rorug07G0066000
rosa_samantha Rh2AG511300 Rh2CG235100 Rh2CG496400 Rh2DG330900 Rh6BG403100 Rh6BG403300 Rh6BG403400 Rh6BG403500 Rh6CG408700 Rh6CG408900 Rh6CG409000 Rh6CG409200 Rh6DG395800 Rh6DG395900 Rh6DG396000 Rh6DG396200 Rh7AG193400 Rh7BG195100 Rh7BG436600 Rh7DG199300 Rh7DG265500
rosa_wichuraiana Rw2G016900 Rw6G034570 Rw6G034580 Rw6G034590 Rw6G034600 Rw7G016900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1058, 3650
AccI GTMKAC 3 cut(s) 1029, 2859, 3321
AccII CGCG 3 cut(s) 238, 688, 690
AciI CCGC 9 cut(s) 236, 686, 1436, 1439, 1571, 1945, 2540, 2813, 3453
AclWI GGATC 9 cut(s) 290, 423, 1147, 1346, 1781, 2058, 3084, 3133, 3286
AcoI YGGCCR 1 cut(s) 1323
AcsI RAATTY 8 cut(s) 553, 842, 1018, 1607, 2848, 2908, 3522, 3711
AcuI CTGAAG 4 cut(s) 870, 2055, 2087, 2781
AdeI CACNNNGTG 1 cut(s) 550
AfaI GTAC 8 cut(s) 1111, 1709, 2896, 2939, 3035, 3134, 3236, 3383
AfiI CCNNNNNNNGG 6 cut(s) 29, 411, 1880, 2400, 2522, 3188
AflIII ACRYGT 2 cut(s) 1274, 1630
AjnI CCWGG 4 cut(s) 1940, 2092, 2329, 2422
AjuI GAANNNNNNNTTGG 8 cut(s) 1898, 1930, 2397, 2429, 2667, 2699, 2802, 2834
AleI CACNNNNGTG 1 cut(s) 3041
Alw21I GWGCWC 3 cut(s) 702, 717, 1892
Alw26I GTCTC 8 cut(s) 418, 1201, 1621, 1683, 1853, 1930, 2910, 3019
AlwI GGATC 9 cut(s) 290, 423, 1147, 1346, 1781, 2058, 3084, 3133, 3286
AlwNI CAGNNNCTG 2 cut(s) 299, 3268
Ama87I CYCGRG 3 cut(s) 22, 701, 1680
AoxI GGCC 8 cut(s) 16, 442, 1323, 1824, 2090, 2524, 2695, 2874
ApeKI GCWGC 9 cut(s) 278, 323, 326, 932, 1481, 2449, 2543, 3178, 3224
ApoI RAATTY 8 cut(s) 553, 842, 1018, 1607, 2848, 2908, 3522, 3711
ArsI GACNNNNNNTTYG 2 cut(s) 375, 407
AseI ATTAAT 1 cut(s) 2736
AspLEI GCGC 3 cut(s) 669, 690, 2454
AspS9I GGNCC 9 cut(s) 17, 141, 233, 442, 482, 683, 1234, 2687, 3570
AsuC2I CCSGG 3 cut(s) 23, 24, 2584
AsuHPI GGTGA 4 cut(s) 451, 1560, 3379, 3641
AvaI CYCGRG 3 cut(s) 22, 701, 1680
AvaII GGWCC 7 cut(s) 141, 233, 482, 683, 1234, 2687, 3570
AxyI CCTNAGG 1 cut(s) 1803
BaeI ACNNNNGTAYC 2 cut(s) 1763, 1796
BalI TGGCCA 1 cut(s) 1325
BanII GRGCYC 4 cut(s) 29, 1454, 1892, 3723
BarI GAAGNNNNNNTAC 6 cut(s) 114, 146, 959, 991, 3374, 3406
BauI CACGAG 1 cut(s) 1524
BbsI GAAGAC 2 cut(s) 2141, 2697
Bbv12I GWGCWC 3 cut(s) 702, 717, 1892
BbvCI CCTCAGC 2 cut(s) 145, 3466
BbvI GCAGC 9 cut(s) 265, 335, 338, 944, 1468, 2461, 2555, 3190, 3211
BcgI CGANNNNNNTGC 2 cut(s) 848, 882
BciT130I CCWGG 4 cut(s) 1942, 2094, 2331, 2424
BciVI GTATCC 1 cut(s) 3659
BclI TGATCA 2 cut(s) 2715, 2880
BcnI CCSGG 3 cut(s) 23, 24, 2584
BcoDI GTCTC 8 cut(s) 418, 1201, 1621, 1683, 1853, 1930, 2910, 3019
BfaI CTAG 3 cut(s) 386, 2057, 3053
BfmI CTRYAG 1 cut(s) 2592
BfuI GTATCC 1 cut(s) 3659
BglI GCCNNNNNGGC 1 cut(s) 24
BglII AGATCT 2 cut(s) 76, 3106
BmcAI AGTACT 2 cut(s) 2896, 2939
Bme1390I CCNGG 7 cut(s) 23, 24, 1942, 2094, 2331, 2424, 2584
Bme18I GGWCC 7 cut(s) 141, 233, 482, 683, 1234, 2687, 3570
BmeT110I CYCGRG 3 cut(s) 22, 701, 1680
BmgT120I GGNCC 9 cut(s) 17, 141, 233, 442, 482, 683, 1234, 2687, 3570
BmiI GGNNCC 7 cut(s) 19, 443, 926, 1235, 1451, 2318, 3085
BmrFI CCNGG 7 cut(s) 23, 24, 1942, 2094, 2331, 2424, 2584
BmrI ACTGGG 7 cut(s) 528, 808, 1042, 1448, 2704, 2953, 3559
BmsI GCATC 7 cut(s) 462, 1089, 1111, 2202, 2521, 2623, 3615
BmuI ACTGGG 7 cut(s) 528, 808, 1042, 1448, 2704, 2953, 3559
BpiI GAAGAC 2 cut(s) 2141, 2697
BplI GAGNNNNNCTC 6 cut(s) 128, 160, 1910, 1942, 2504, 2536
BpmI CTGGAG 2 cut(s) 1760, 2445
Bpu10I CCTNAGC 2 cut(s) 145, 3466
BpuMI CCSGG 3 cut(s) 23, 24, 2584
BsaBI GATNNNNATC 2 cut(s) 300, 1286
BsaI GGTCTC 1 cut(s) 1853
BsaJI CCNNGG 8 cut(s) 21, 22, 405, 1113, 1827, 2329, 2330, 3255
BsaWI WCCGGW 1 cut(s) 133
BsaXI ACNNNNNCTCC 6 cut(s) 1653, 1683, 1848, 1878, 3303, 3333
Bsc4I CCNNNNNNNGG 6 cut(s) 29, 411, 1880, 2400, 2522, 3188
Bse21I CCTNAGG 1 cut(s) 1803
Bse3DI GCAATG 3 cut(s) 861, 3126, 3447
Bse8I GATNNNNATC 2 cut(s) 300, 1286
BseBI CCWGG 4 cut(s) 1942, 2094, 2331, 2424
BseDI CCNNGG 8 cut(s) 21, 22, 405, 1113, 1827, 2329, 2330, 3255
BseGI GGATG 9 cut(s) 123, 511, 570, 895, 2155, 2492, 2515, 2604, 2787
BseJI GATNNNNATC 2 cut(s) 300, 1286
BseLI CCNNNNNNNGG 6 cut(s) 29, 411, 1880, 2400, 2522, 3188
BseMI GCAATG 3 cut(s) 861, 3126, 3447
BseRI GAGGAG 4 cut(s) 644, 2265, 2682, 2889
BseXI GCAGC 9 cut(s) 265, 335, 338, 944, 1468, 2461, 2555, 3190, 3211
BseYI CCCAGC 1 cut(s) 928
BsgI GTGCAG 4 cut(s) 2148, 2584, 3039, 3197
Bsh1236I CGCG 3 cut(s) 238, 688, 690
BshFI GGCC 8 cut(s) 18, 444, 1325, 1826, 2092, 2526, 2697, 2876
BsiHKAI GWGCWC 3 cut(s) 702, 717, 1892
BsiHKCI CYCGRG 3 cut(s) 22, 701, 1680
BsiSI CCGG 3 cut(s) 23, 134, 2583
BslFI GGGAC 3 cut(s) 396, 1247, 3273
BslI CCNNNNNNNGG 6 cut(s) 29, 411, 1880, 2400, 2522, 3188
BsmAI GTCTC 8 cut(s) 418, 1201, 1621, 1683, 1853, 1930, 2910, 3019
BsmBI CGTCTC 1 cut(s) 418
BsmFI GGGAC 3 cut(s) 396, 1247, 3273
BsmI GAATGC 1 cut(s) 1594
BsnI GGCC 8 cut(s) 18, 444, 1325, 1826, 2092, 2526, 2697, 2876
Bso31I GGTCTC 1 cut(s) 1853
BsoBI CYCGRG 3 cut(s) 22, 701, 1680
Bsp1286I GDGCHC 6 cut(s) 29, 702, 717, 1454, 1892, 3723
Bsp1407I TGTACA 1 cut(s) 3033
Bsp19I CCATGG 1 cut(s) 405
BspACI CCGC 9 cut(s) 236, 686, 1436, 1439, 1571, 1945, 2540, 2813, 3453
BspANI GGCC 8 cut(s) 18, 444, 1325, 1826, 2092, 2526, 2697, 2876
BspFNI CGCG 3 cut(s) 238, 688, 690
BspHI TCATGA 1 cut(s) 1203
BspLI GGNNCC 7 cut(s) 19, 443, 926, 1235, 1451, 2318, 3085
BspPI GGATC 9 cut(s) 290, 423, 1147, 1346, 1781, 2058, 3084, 3133, 3286
BspTNI GGTCTC 1 cut(s) 1853
BsrDI GCAATG 3 cut(s) 861, 3126, 3447
BsrGI TGTACA 1 cut(s) 3033
BssECI CCNNGG 8 cut(s) 21, 22, 405, 1113, 1827, 2329, 2330, 3255
BssNAI GTATAC 2 cut(s) 2860, 3322
BssSI CACGAG 1 cut(s) 1524
BssT1I CCWWGG 3 cut(s) 405, 1827, 3255
Bst1107I GTATAC 2 cut(s) 2860, 3322
Bst2BI CACGAG 1 cut(s) 1524
Bst2UI CCWGG 4 cut(s) 1942, 2094, 2331, 2424
Bst6I CTCTTC 6 cut(s) 117, 262, 1007, 1247, 1827, 3152
BstAPI GCANNNNNTGC 1 cut(s) 2970
BstAUI TGTACA 1 cut(s) 3033
BstC8I GCNNGC 5 cut(s) 161, 204, 897, 2236, 2971
BstDSI CCRYGG 2 cut(s) 405, 1113
BstENI CCTNNNNNAGG 1 cut(s) 2398
BstF5I GGATG 9 cut(s) 123, 511, 570, 895, 2155, 2492, 2515, 2604, 2787
BstFNI CGCG 3 cut(s) 238, 688, 690
BstHHI GCGC 3 cut(s) 669, 690, 2454
BstMAI GTCTC 8 cut(s) 418, 1201, 1621, 1683, 1853, 1930, 2910, 3019
BstMWI GCNNNNNNNGC 9 cut(s) 24, 212, 2323, 2458, 2540, 2970, 3047, 3458, 3467
BstNI CCWGG 4 cut(s) 1942, 2094, 2331, 2424
BstNSI RCATGY 4 cut(s) 1278, 1634, 2017, 3707
BstSCI CCNGG 7 cut(s) 21, 22, 1940, 2092, 2329, 2422, 2582
BstSFI CTRYAG 1 cut(s) 2592
BstUI CGCG 3 cut(s) 238, 688, 690
BstV1I GCAGC 9 cut(s) 265, 335, 338, 944, 1468, 2461, 2555, 3190, 3211
BstV2I GAAGAC 2 cut(s) 2141, 2697
BstX2I RGATCY 6 cut(s) 76, 295, 1139, 3076, 3106, 3125
BstXI CCANNNNNNTGG 2 cut(s) 3534, 3567
BstYI RGATCY 6 cut(s) 76, 295, 1139, 3076, 3106, 3125
BstZ17I GTATAC 2 cut(s) 2860, 3322
Bsu36I CCTNAGG 1 cut(s) 1803
BsuI GTATCC 1 cut(s) 3659
BsuRI GGCC 8 cut(s) 18, 444, 1325, 1826, 2092, 2526, 2697, 2876
BtgI CCRYGG 2 cut(s) 405, 1113
BtsCI GGATG 9 cut(s) 123, 511, 570, 895, 2155, 2492, 2515, 2604, 2787
BtsI GCAGTG 3 cut(s) 5, 3027, 3036
Cac8I GCNNGC 5 cut(s) 161, 204, 897, 2236, 2971
CaiI CAGNNNCTG 2 cut(s) 299, 3268
CciI TCATGA 1 cut(s) 1203
CfoI GCGC 3 cut(s) 669, 690, 2454
Cfr13I GGNCC 9 cut(s) 17, 141, 233, 442, 482, 683, 1234, 2687, 3570
Cfr9I CCCGGG 1 cut(s) 22
Csp6I GTAC 8 cut(s) 1110, 1708, 2895, 2938, 3034, 3133, 3235, 3382
CspCI CAANNNNNGTGG 2 cut(s) 889, 924
CviQI GTAC 8 cut(s) 1110, 1708, 2895, 2938, 3034, 3133, 3235, 3382
DraIII CACNNNGTG 1 cut(s) 550
EaeI YGGCCR 1 cut(s) 1323
Eam1104I CTCTTC 6 cut(s) 117, 262, 1007, 1247, 1827, 3152
EarI CTCTTC 6 cut(s) 117, 262, 1007, 1247, 1827, 3152
Ecl136II GAGCTC 1 cut(s) 1890
Eco130I CCWWGG 3 cut(s) 405, 1827, 3255
Eco147I AGGCCT 3 cut(s) 1826, 2092, 2876
Eco24I GRGCYC 4 cut(s) 29, 1454, 1892, 3723
Eco31I GGTCTC 1 cut(s) 1853
Eco32I GATATC 2 cut(s) 513, 2210
Eco47I GGWCC 7 cut(s) 141, 233, 482, 683, 1234, 2687, 3570
Eco53kI GAGCTC 1 cut(s) 1890
Eco57I CTGAAG 4 cut(s) 870, 2055, 2087, 2781
Eco81I CCTNAGG 1 cut(s) 1803
Eco88I CYCGRG 3 cut(s) 22, 701, 1680
EcoICRI GAGCTC 1 cut(s) 1890
EcoNI CCTNNNNNAGG 1 cut(s) 2398
EcoO109I RGGNCCY 3 cut(s) 17, 141, 1234
EcoRI GAATTC 4 cut(s) 553, 1018, 2848, 3522
EcoRII CCWGG 4 cut(s) 1940, 2092, 2329, 2422
EcoRV GATATC 2 cut(s) 513, 2210
EcoT14I CCWWGG 3 cut(s) 405, 1827, 3255
EcoT22I ATGCAT 2 cut(s) 1104, 1594
EcoT38I GRGCYC 4 cut(s) 29, 1454, 1892, 3723
ErhI CCWWGG 3 cut(s) 405, 1827, 3255
Esp3I CGTCTC 1 cut(s) 418
FalI AAGNNNNNCTT 6 cut(s) 990, 1022, 1824, 1856, 2802, 2834
FaqI GGGAC 3 cut(s) 396, 1247, 3273
FauNDI CATATG 2 cut(s) 1579, 3250
FbaI TGATCA 2 cut(s) 2715, 2880
FblI GTMKAC 3 cut(s) 1029, 2859, 3321
FokI GGATG 9 cut(s) 130, 518, 557, 902, 2162, 2479, 2522, 2591, 2794
FriOI GRGCYC 4 cut(s) 29, 1454, 1892, 3723
FspBI CTAG 3 cut(s) 386, 2057, 3053
GlaI GCGC 3 cut(s) 668, 689, 2453
GsaI CCCAGC 1 cut(s) 932
GsuI CTGGAG 2 cut(s) 1760, 2445
HaeIII GGCC 8 cut(s) 18, 444, 1325, 1826, 2092, 2526, 2697, 2876
HapII CCGG 3 cut(s) 23, 134, 2583
HhaI GCGC 3 cut(s) 669, 690, 2454
Hin6I GCGC 3 cut(s) 667, 688, 2452
HinP1I GCGC 3 cut(s) 667, 688, 2452
HincII GTYRAC 1 cut(s) 2011
HindII GTYRAC 1 cut(s) 2011
HindIII AAGCTT 2 cut(s) 981, 1308
HinfI GANTC 6 cut(s) 456, 1179, 1287, 2294, 2339, 3623
HpaII CCGG 3 cut(s) 23, 134, 2583
HphI GGTGA 4 cut(s) 451, 1560, 3379, 3641
Hpy99I CGWCG 1 cut(s) 1110
HpyCH4IV ACGT 3 cut(s) 1108, 1985, 3489
HpyF10VI GCNNNNNNNGC 9 cut(s) 24, 212, 2323, 2458, 2540, 2970, 3047, 3458, 3467
HpySE526I ACGT 3 cut(s) 1108, 1985, 3489
HspAI GCGC 3 cut(s) 667, 688, 2452
Ksp22I TGATCA 2 cut(s) 2715, 2880
LmnI GCTCC 4 cut(s) 1449, 2322, 2377, 3089
Lsp1109I GCAGC 9 cut(s) 265, 335, 338, 944, 1468, 2461, 2555, 3190, 3211
LweI GCATC 7 cut(s) 462, 1089, 1111, 2202, 2521, 2623, 3615
MaeI CTAG 3 cut(s) 386, 2057, 3053
MaeII ACGT 3 cut(s) 1108, 1985, 3489
MaeIII GTNAC 6 cut(s) 97, 355, 769, 829, 2281, 2791
MfeI CAATTG 2 cut(s) 2547, 3696
MflI RGATCY 6 cut(s) 76, 295, 1139, 3076, 3106, 3125
MhlI GDGCHC 6 cut(s) 29, 702, 717, 1454, 1892, 3723
MlsI TGGCCA 1 cut(s) 1325
MluNI TGGCCA 1 cut(s) 1325
MlyI GAGTC 2 cut(s) 450, 2303
MmeI TCCRAC 8 cut(s) 211, 460, 504, 1604, 1876, 2395, 2766, 3688
Mox20I TGGCCA 1 cut(s) 1325
Mph1103I ATGCAT 2 cut(s) 1104, 1594
MscI TGGCCA 1 cut(s) 1325
MseI TTAA 7 cut(s) 288, 938, 2144, 2736, 2826, 3114, 3759
MslI CAYNNNNRTG 4 cut(s) 1058, 1157, 1319, 3041
Msp20I TGGCCA 1 cut(s) 1325
MspA1I CMGCKG 2 cut(s) 1573, 3455
MspI CCGG 3 cut(s) 23, 134, 2583
MspR9I CCNGG 7 cut(s) 23, 24, 1942, 2094, 2331, 2424, 2584
MunI CAATTG 2 cut(s) 2547, 3696
Mva1269I GAATGC 1 cut(s) 1594
MvaI CCWGG 4 cut(s) 1942, 2094, 2331, 2424
MvnI CGCG 3 cut(s) 238, 688, 690
MwoI GCNNNNNNNGC 9 cut(s) 24, 212, 2323, 2458, 2540, 2970, 3047, 3458, 3467
NciI CCSGG 3 cut(s) 23, 24, 2584
NcoI CCATGG 1 cut(s) 405
NdeI CATATG 2 cut(s) 1579, 3250
NlaIV GGNNCC 7 cut(s) 19, 443, 926, 1235, 1451, 2318, 3085
NmuCI GTSAC 2 cut(s) 769, 2281
NsiI ATGCAT 2 cut(s) 1104, 1594
NspI RCATGY 4 cut(s) 1278, 1634, 2017, 3707
OliI CACNNNNGTG 1 cut(s) 3041
PaeR7I CTCGAG 2 cut(s) 701, 1680
PagI TCATGA 1 cut(s) 1203
PasI CCCWGGG 1 cut(s) 2330
PceI AGGCCT 3 cut(s) 1826, 2092, 2876
PciI ACATGT 2 cut(s) 1274, 1630
PctI GAATGC 1 cut(s) 1594
PfeI GAWTC 4 cut(s) 1179, 1287, 2339, 3623
PleI GAGTC 2 cut(s) 450, 2302
PpsI GAGTC 2 cut(s) 450, 2302
PpuMI RGGWCCY 2 cut(s) 141, 1234
PscI ACATGT 2 cut(s) 1274, 1630
PshBI ATTAAT 1 cut(s) 2736
PsiI TTATAA 2 cut(s) 1058, 3650
Psp124BI GAGCTC 1 cut(s) 1892
Psp5II RGGWCCY 2 cut(s) 141, 1234
Psp6I CCWGG 4 cut(s) 1940, 2092, 2329, 2422
PspFI CCCAGC 1 cut(s) 928
PspGI CCWGG 4 cut(s) 1940, 2092, 2329, 2422
PspN4I GGNNCC 7 cut(s) 19, 443, 926, 1235, 1451, 2318, 3085
PspPI GGNCC 9 cut(s) 17, 141, 233, 442, 482, 683, 1234, 2687, 3570
PspPPI RGGWCCY 2 cut(s) 141, 1234
PspXI VCTCGAGB 1 cut(s) 701
PstNI CAGNNNCTG 2 cut(s) 299, 3268
PsuI RGATCY 6 cut(s) 76, 295, 1139, 3076, 3106, 3125
RsaI GTAC 8 cut(s) 1111, 1709, 2896, 2939, 3035, 3134, 3236, 3383
RsaNI GTAC 8 cut(s) 1110, 1708, 2895, 2938, 3034, 3133, 3235, 3382
RseI CAYNNNNRTG 4 cut(s) 1058, 1157, 1319, 3041
SacI GAGCTC 1 cut(s) 1892
SaqAI TTAA 7 cut(s) 288, 938, 2144, 2736, 2826, 3114, 3759
Sau96I GGNCC 9 cut(s) 17, 141, 233, 442, 482, 683, 1234, 2687, 3570
ScaI AGTACT 2 cut(s) 2896, 2939
SchI GAGTC 2 cut(s) 450, 2303
ScrFI CCNGG 7 cut(s) 23, 24, 1942, 2094, 2331, 2424, 2584
SduI GDGCHC 6 cut(s) 29, 702, 717, 1454, 1892, 3723
SfaNI GCATC 7 cut(s) 462, 1089, 1111, 2202, 2521, 2623, 3615
SfcI CTRYAG 1 cut(s) 2592
Sfr274I CTCGAG 2 cut(s) 701, 1680
SinI GGWCC 7 cut(s) 141, 233, 482, 683, 1234, 2687, 3570
SlaI CTCGAG 2 cut(s) 701, 1680
SmaI CCCGGG 1 cut(s) 24
SmiMI CAYNNNNRTG 4 cut(s) 1058, 1157, 1319, 3041
SmlI CTYRAG 2 cut(s) 701, 1680
SmoI CTYRAG 2 cut(s) 701, 1680
SseBI AGGCCT 3 cut(s) 1826, 2092, 2876
SsiI CCGC 9 cut(s) 236, 686, 1436, 1439, 1571, 1945, 2540, 2813, 3453
SspI AATATT 3 cut(s) 286, 2502, 3069
SspMI CTAG 3 cut(s) 386, 2057, 3053
SstI GAGCTC 1 cut(s) 1892
StuI AGGCCT 3 cut(s) 1826, 2092, 2876
StyD4I CCNGG 7 cut(s) 21, 22, 1940, 2092, 2329, 2422, 2582
StyI CCWWGG 3 cut(s) 405, 1827, 3255
TaiI ACGT 3 cut(s) 1111, 1988, 3492
TaqI TCGA 5 cut(s) 702, 1105, 1681, 2184, 3290
TatI WGTACW 6 cut(s) 2894, 2937, 3033, 3132, 3234, 3381
TauI GCSGC 5 cut(s) 1439, 1948, 2543, 2816, 3455
TfiI GAWTC 4 cut(s) 1179, 1287, 2339, 3623
Tru1I TTAA 7 cut(s) 288, 938, 2144, 2736, 2826, 3114, 3759
Tru9I TTAA 7 cut(s) 288, 938, 2144, 2736, 2826, 3114, 3759
TseFI GTSAC 2 cut(s) 769, 2281
TseI GCWGC 9 cut(s) 278, 323, 326, 932, 1481, 2449, 2543, 3178, 3224
Tsp45I GTSAC 2 cut(s) 769, 2281
TspGWI ACGGA 2 cut(s) 123, 2179
TspMI CCCGGG 1 cut(s) 22
VpaK11BI GGWCC 7 cut(s) 141, 233, 482, 683, 1234, 2687, 3570
VspI ATTAAT 1 cut(s) 2736
XagI CCTNNNNNAGG 1 cut(s) 2398
XapI RAATTY 8 cut(s) 553, 842, 1018, 1607, 2848, 2908, 3522, 3711
XceI RCATGY 4 cut(s) 1278, 1634, 2017, 3707
XcmI CCANNNNNNNNNTGG 5 cut(s) 46, 581, 1701, 1825, 2503
XhoI CTCGAG 2 cut(s) 701, 1680
XmaI CCCGGG 1 cut(s) 22
XmiI GTMKAC 3 cut(s) 1029, 2859, 3321
XspI CTAG 3 cut(s) 386, 2057, 3053
ZrmI AGTACT 2 cut(s) 2896, 2939
Zsp2I ATGCAT 2 cut(s) 1104, 1594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.