FvH4_4g21351

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000591
Physical Location & Seq
Forward (+)
24398674 .. 24399317
644 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g21351.t1

Sequence Viewer

Length: 531 bp
ATGATGATTATTTGGTCGGTTTGGAGGAACCGAAACGATCACATCTGGAATAATAATGCAAAGCAGGCTACTGAGGTAGTACCTCTTACCCTGGGTTGGTGGGAGGAGTTTAAATCTACAATTGTGAAAAGCAGAGGTCGCAATGTCTCTCCTCGGGACAGATGGATCACCCCTTCCTCGGGGTACATCAAACTGAATGTTGATGCAACCTACAACAATGCATCTGCAAGAGTGCAGGTAGCGATCCAGCATAATCTCTATCCTCTTACCATTGAGACAGATTGCTTGGTTTTGGTTTCGGCCCTTACCAGTGGCTCCCTTGATGATTCTGAACTTGGATTCCTAATTGAAGACTTGCGGGCAAGCTTGTCTACTATTACTGCTCTAGTTCGGTTTGTGAGAAGATCAGCAAACAGAGTTGCTCATGTTATAGCCAAAGAGGCTTTTAGTGGTCCTGCTAATTTAGTTTTTACTACCGATCCTTCTCGTGCTGTGAGAAGGATGCTTTCTTCTGATTATAATGATATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PfkB TB2_DP1_HVA22

Protein Analysis

177

Amino Acids

19.86

Weight (kDa)

9.24

Isoelectric Point (pI)

50.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 78 - 148 2.1e-08 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000591)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03566
fragaria_vesca FvH4_2g12622 FvH4_2g12642 FvH4_2g13902 FvH4_3g05011 FvH4_3g18961 FvH4_3g31940 FvH4_4g08421 FvH4_4g21351 FvH4_5g28071 FvH4_5g34871 FvH4_6g21812 FvH4_6g30441 FvH4_6g33631
rosa_chinensis RchiOBHm_Chr1g0320221 RchiOBHm_Chr1g0356521 RchiOBHm_Chr4g0389021 RchiOBHm_Chr5g0020651 RchiOBHm_Chr5g0027511
rosa_laevigata RLG00000002966 RLG00000019504
rosa_multiflora Rmu_co7963640.1_g000001 Rmu_co7988458.1_g000001 Rmu_co8109512.1_g000001 Rmu_co8136160.1_g000001 Rmu_co8253655.1_g000001 Rmu_sc0000388.1_g000040 Rmu_sc0000540.1_g000056 Rmu_sc0000574.1_g000013 Rmu_sc0000623.1_g000001 Rmu_sc0000795.1_g000005 Rmu_sc0000795.1_g000006 Rmu_sc0001063.1_g000001 Rmu_sc0002116.1_g000001 Rmu_sc0002192.1_g000012 Rmu_sc0002640.1_g000015 Rmu_sc0003337.1_g000039 Rmu_sc0004298.1_g000003 Rmu_sc0004771.1_g000001 Rmu_sc0006119.1_g000010 Rmu_sc0006633.1_g000007 Rmu_sc0007025.1_g000016 Rmu_sc0007221.1_g000002 Rmu_sc0007222.1_g000002 Rmu_sc0007806.1_g000016 Rmu_sc0008241.1_g000018 Rmu_sc0008348.1_g000001 Rmu_sc0009034.1_g000001 Rmu_sc0009955.1_g000004 Rmu_sc0018325.1_g000009 Rmu_sc0028328.1_g000002 Rmu_sc0030178.1_g000001 Rmu_sc0034381.1_g000001 Rmu_sc0041085.1_g000001 Rmu_sc0041085.1_g000002
rosa_roxburghii Rroxscaffold_3G00233230 Rroxscaffold_7G00193120 Rroxscaffold_7G00194820
rosa_rugosa Rorug01G0017000 Rorug01G0036400 Rorug01G0062600 Rorug01G0118500 Rorug02G0351500 Rorug02G0497600 Rorug03G0233200 Rorug03G0279500 Rorug03G0347600 Rorug04G0077100 Rorug04G0077200 Rorug04G0210900 Rorug05G0206900 Rorug07G0202900 Rorug07G0334000
rosa_samantha Rh2CG195700 Rh4DG035800
rosa_wichuraiana Rw0G005080 Rw0G013600 Rw2G020080 Rw2G024380 Rw5G002230 Rw5G036790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 519
Acc36I ACCTGC 1 cut(s) 226
AccI GTMKAC 1 cut(s) 371
AciI CCGC 1 cut(s) 358
AclWI GGATC 3 cut(s) 173, 238, 473
AfaI GTAC 2 cut(s) 81, 185
AfiI CCNNNNNNNGG 3 cut(s) 96, 178, 179
AgsI TTSAA 1 cut(s) 350
AjnI CCWGG 1 cut(s) 90
AloI GAACNNNNNNTCC 2 cut(s) 324, 356
AluBI AGCT 1 cut(s) 366
AluI AGCT 1 cut(s) 366
Alw26I GTCTC 2 cut(s) 151, 269
AlwI GGATC 3 cut(s) 173, 238, 473
Ama87I CYCGRG 2 cut(s) 153, 178
AoxI GGCC 1 cut(s) 300
AspS9I GGNCC 2 cut(s) 301, 452
AsuHPI GGTGA 1 cut(s) 160
AvaI CYCGRG 2 cut(s) 153, 178
AvaII GGWCC 1 cut(s) 452
BauI CACGAG 1 cut(s) 486
BbsI GAAGAC 1 cut(s) 357
BccI CCATC 1 cut(s) 156
BciT130I CCWGG 1 cut(s) 92
BcoDI GTCTC 2 cut(s) 151, 269
BfaI CTAG 1 cut(s) 386
BfuAI ACCTGC 1 cut(s) 226
BglI GCCNNNNNGGC 1 cut(s) 440
Bme1390I CCNGG 1 cut(s) 92
Bme18I GGWCC 1 cut(s) 452
BmeT110I CYCGRG 2 cut(s) 153, 178
BmgT120I GGNCC 2 cut(s) 301, 452
BmiI GGNNCC 2 cut(s) 29, 316
BmrFI CCNGG 1 cut(s) 92
BmsI GCATC 3 cut(s) 193, 230, 492
BpiI GAAGAC 1 cut(s) 357
BsaJI CCNNGG 4 cut(s) 90, 91, 152, 177
Bsc4I CCNNNNNNNGG 3 cut(s) 96, 178, 179
Bse1I ACTGG 1 cut(s) 309
Bse3DI GCAATG 1 cut(s) 148
BseBI CCWGG 1 cut(s) 92
BseDI CCNNGG 4 cut(s) 90, 91, 152, 177
BseGI GGATG 1 cut(s) 507
BseLI CCNNNNNNNGG 3 cut(s) 96, 178, 179
BseMI GCAATG 1 cut(s) 148
BseMII CTCAG 1 cut(s) 63
BseNI ACTGG 1 cut(s) 309
BseRI GAGGAG 2 cut(s) 119, 141
BsgI GTGCAG 1 cut(s) 254
BshFI GGCC 1 cut(s) 302
BsiHKCI CYCGRG 2 cut(s) 153, 178
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 3 cut(s) 96, 178, 179
BsmAI GTCTC 2 cut(s) 151, 269
BsmFI GGGAC 1 cut(s) 170
BsnI GGCC 1 cut(s) 302
BsoBI CYCGRG 2 cut(s) 153, 178
Bsp143I GATC 5 cut(s) 37, 165, 243, 404, 478
BspACI CCGC 1 cut(s) 358
BspANI GGCC 1 cut(s) 302
BspCNI CTCAG 1 cut(s) 64
BspLI GGNNCC 2 cut(s) 29, 316
BspMI ACCTGC 1 cut(s) 226
BspPI GGATC 3 cut(s) 173, 238, 473
BsrDI GCAATG 1 cut(s) 148
BsrI ACTGG 1 cut(s) 309
BssECI CCNNGG 4 cut(s) 90, 91, 152, 177
BssMI GATC 5 cut(s) 37, 165, 243, 404, 478
BssSI CACGAG 1 cut(s) 486
Bst2BI CACGAG 1 cut(s) 486
Bst2UI CCWGG 1 cut(s) 92
BstC8I GCNNGC 3 cut(s) 66, 360, 364
BstDEI CTNAG 1 cut(s) 72
BstF5I GGATG 1 cut(s) 507
BstKTI GATC 5 cut(s) 40, 168, 246, 407, 481
BstMAI GTCTC 2 cut(s) 151, 269
BstMBI GATC 5 cut(s) 37, 165, 243, 404, 478
BstMWI GCNNNNNNNGC 3 cut(s) 65, 138, 440
BstNI CCWGG 1 cut(s) 92
BstSCI CCNGG 1 cut(s) 90
BstV2I GAAGAC 1 cut(s) 357
BsuRI GGCC 1 cut(s) 302
BtsCI GGATG 1 cut(s) 507
BtsIMutI CAGTG 1 cut(s) 316
BveI ACCTGC 1 cut(s) 226
Cac8I GCNNGC 3 cut(s) 66, 360, 364
Cfr13I GGNCC 2 cut(s) 301, 452
Csp6I GTAC 2 cut(s) 80, 184
CviAII CATG 1 cut(s) 425
CviJI RGCY 6 cut(s) 68, 302, 315, 366, 434, 443
CviKI_1 RGCY 6 cut(s) 68, 302, 315, 366, 434, 443
CviQI GTAC 2 cut(s) 80, 184
DdeI CTNAG 1 cut(s) 72
DpnI GATC 5 cut(s) 39, 167, 245, 406, 480
DpnII GATC 5 cut(s) 37, 165, 243, 404, 478
DraI TTTAAA 1 cut(s) 112
Eco47I GGWCC 1 cut(s) 452
Eco88I CYCGRG 2 cut(s) 153, 178
EcoRII CCWGG 1 cut(s) 90
EcoT22I ATGCAT 1 cut(s) 223
FaeI CATG 1 cut(s) 428
FaiI YATR 4 cut(s) 252, 426, 431, 519
FaqI GGGAC 1 cut(s) 170
FatI CATG 1 cut(s) 424
FauI CCCGC 1 cut(s) 351
FblI GTMKAC 1 cut(s) 371
FokI GGATG 1 cut(s) 514
FspBI CTAG 1 cut(s) 386
HaeIII GGCC 1 cut(s) 302
Hin1II CATG 1 cut(s) 428
HindIII AAGCTT 1 cut(s) 364
HinfI GANTC 2 cut(s) 326, 339
HphI GGTGA 1 cut(s) 160
Hpy166II GTNNAC 1 cut(s) 372
Hpy188I TCNGA 2 cut(s) 331, 514
Hpy188III TCNNGA 2 cut(s) 46, 155
Hpy8I GTNNAC 1 cut(s) 372
HpyAV CCTTC 3 cut(s) 183, 492, 492
HpyCH4V TGCA 5 cut(s) 59, 206, 221, 227, 235
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 138, 440
HpyF3I CTNAG 1 cut(s) 72
Hsp92II CATG 1 cut(s) 428
Kzo9I GATC 5 cut(s) 37, 165, 243, 404, 478
LmnI GCTCC 1 cut(s) 320
LpnPI CCDG 8 cut(s) 31, 50, 77, 104, 221, 260, 322, 468
LweI GCATC 3 cut(s) 193, 230, 492
MaeI CTAG 1 cut(s) 386
MalI GATC 5 cut(s) 39, 167, 245, 406, 480
MboI GATC 5 cut(s) 37, 165, 243, 404, 478
MboII GAAGA 3 cut(s) 362, 414, 501
MfeI CAATTG 1 cut(s) 120
MluCI AATT 3 cut(s) 120, 345, 460
MnlI CCTC 9 cut(s) 18, 67, 93, 97, 128, 162, 187, 273, 433
Mph1103I ATGCAT 1 cut(s) 223
MseI TTAA 1 cut(s) 111
MspR9I CCNGG 1 cut(s) 92
MunI CAATTG 1 cut(s) 120
MvaI CCWGG 1 cut(s) 92
MwoI GCNNNNNNNGC 3 cut(s) 65, 138, 440
NdeII GATC 5 cut(s) 37, 165, 243, 404, 478
NlaIII CATG 1 cut(s) 428
NlaIV GGNNCC 2 cut(s) 29, 316
NsiI ATGCAT 1 cut(s) 223
PasI CCCWGGG 1 cut(s) 91
PfeI GAWTC 2 cut(s) 326, 339
PsiI TTATAA 1 cut(s) 519
Psp6I CCWGG 1 cut(s) 90
PspGI CCWGG 1 cut(s) 90
PspN4I GGNNCC 2 cut(s) 29, 316
PspPI GGNCC 2 cut(s) 301, 452
RsaI GTAC 2 cut(s) 81, 185
RsaNI GTAC 2 cut(s) 80, 184
SaqAI TTAA 1 cut(s) 111
Sau3AI GATC 5 cut(s) 37, 165, 243, 404, 478
Sau96I GGNCC 2 cut(s) 301, 452
ScrFI CCNGG 1 cut(s) 92
SetI ASST 6 cut(s) 78, 85, 139, 212, 240, 368
SfaNI GCATC 3 cut(s) 193, 230, 492
SinI GGWCC 1 cut(s) 452
Sse9I AATT 3 cut(s) 120, 345, 460
SsiI CCGC 1 cut(s) 358
SspMI CTAG 1 cut(s) 386
StyD4I CCNGG 1 cut(s) 90
TasI AATT 3 cut(s) 120, 345, 460
TfiI GAWTC 2 cut(s) 326, 339
Tru1I TTAA 1 cut(s) 111
Tru9I TTAA 1 cut(s) 111
TscAI CASTG 1 cut(s) 316
TspRI CASTG 1 cut(s) 316
VpaK11BI GGWCC 1 cut(s) 452
XmiI GTMKAC 1 cut(s) 371
XspI CTAG 1 cut(s) 386
Zsp2I ATGCAT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.