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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AgsI | TTSAA | 2 cut(s) | 38, 89 |
| AluBI | AGCT | 2 cut(s) | 13, 59 |
| AluI | AGCT | 2 cut(s) | 13, 59 |
| BanII | GRGCYC | 1 cut(s) | 30 |
| BbsI | GAAGAC | 1 cut(s) | 132 |
| BmiI | GGNNCC | 1 cut(s) | 29 |
| BmsI | GCATC | 1 cut(s) | 9 |
| BpiI | GAAGAC | 1 cut(s) | 132 |
| BseRI | GAGGAG | 1 cut(s) | 20 |
| Bsp1286I | GDGCHC | 1 cut(s) | 30 |
| BspLI | GGNNCC | 1 cut(s) | 29 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 19 |
| BstV2I | GAAGAC | 1 cut(s) | 132 |
| CviAII | CATG | 1 cut(s) | 130 |
| CviJI | RGCY | 3 cut(s) | 13, 28, 59 |
| CviKI_1 | RGCY | 3 cut(s) | 13, 28, 59 |
| Eco24I | GRGCYC | 1 cut(s) | 30 |
| EcoT38I | GRGCYC | 1 cut(s) | 30 |
| FaeI | CATG | 1 cut(s) | 133 |
| FaiI | YATR | 3 cut(s) | 8, 131, 137 |
| FatI | CATG | 1 cut(s) | 129 |
| FriOI | GRGCYC | 1 cut(s) | 30 |
| Hin1II | CATG | 1 cut(s) | 133 |
| HindIII | AAGCTT | 1 cut(s) | 57 |
| HinfI | GANTC | 1 cut(s) | 38 |
| Hpy188III | TCNNGA | 1 cut(s) | 16 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 19 |
| Hsp92II | CATG | 1 cut(s) | 133 |
| LmnI | GCTCC | 1 cut(s) | 33 |
| LweI | GCATC | 1 cut(s) | 9 |
| MboII | GAAGA | 1 cut(s) | 137 |
| MhlI | GDGCHC | 1 cut(s) | 30 |
| MluCI | AATT | 1 cut(s) | 48 |
| MmeI | TCCRAC | 1 cut(s) | 78 |
| MnlI | CCTC | 3 cut(s) | 41, 94, 115 |
| MslI | CAYNNNNRTG | 1 cut(s) | 134 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 19 |
| NlaIII | CATG | 1 cut(s) | 133 |
| NlaIV | GGNNCC | 1 cut(s) | 29 |
| PfeI | GAWTC | 1 cut(s) | 38 |
| PspN4I | GGNNCC | 1 cut(s) | 29 |
| RseI | CAYNNNNRTG | 1 cut(s) | 134 |
| SduI | GDGCHC | 1 cut(s) | 30 |
| SetI | ASST | 3 cut(s) | 15, 61, 105 |
| SfaNI | GCATC | 1 cut(s) | 9 |
| SgeI | CNNG | 4 cut(s) | 28, 35, 54, 142 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 134 |
| Sse9I | AATT | 1 cut(s) | 48 |
| SspI | AATATT | 1 cut(s) | 85 |
| TasI | AATT | 1 cut(s) | 48 |
| TfiI | GAWTC | 1 cut(s) | 38 |
| TspDTI | ATGAA | 3 cut(s) | 23, 69, 146 |